CN106620878A - Collagen-chitosan composite material capable of slowly releasing icariin and preparation method thereof - Google Patents
Collagen-chitosan composite material capable of slowly releasing icariin and preparation method thereof Download PDFInfo
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Abstract
The invention provides a collagen-chitosan composite material capable of slowly releasing icariin and a preparation method thereof. The preparation method comprises the following steps: S1, mixing 0.336 percent of collagen solution and 2 percent of chitosan solution in a volume ratio of 5:5 to 6:4, pouring the mixture into a scaffold mold, performing freeze drying, and soaking in alcohol of different concentrations in sequence for gradient deacidification; S2, adding 0.5 percent of genipin to crosslink for 24 hours, and cleaning to obtain a collagen-chitosan scaffold material; S3, uniformly dripping a 0.005 to 0.5mg/mL icariin solution into the collagen-chitosan scaffold material, and performing freeze drying to obtain the collagen-chitosan composite material capable of slowly releasing the icariin. The preparation method of the scaffold material is simple and convenient, and the material cost is low; the collagen-chitosan composite material capable of slowly releasing the icariin prepared by the method can effectively release icariin for a long time; the scaffold material has a high medicament slow release feature and higher biosecurity.
Description
Technical field
The present invention relates to biomedical material applied technical field, more particularly, to a kind of slow release icariin
Collagen-chitin composite and its preparation method and application.
Background technology
Periodontitis are the chronic inflammatory diseases of periodontal tissue, often result in the damage of periodontal tissue, cause frontal resorption finally to lead
Cause absence of tooth commonly encountered diseases because.There is some defects and deficiency, such as collagen-chitosan in the material for being applied to Alveolar Bone Defect reparation
The good biocompatibility of sugared timbering material, beneficial to cell adhesion and propagation but its skeletonization effect on driving birds is not good.Such as by hydroxyapatite or
The materials such as tricalcium phosphate combine to form composite with collagen-chitin support, due to hydroxyapatite or tricalcium phosphate granule
After contacting or swallowed with immunocyte such as mononuclear cell, there is the risk for causing local organization aseptic inflammation.Limit these
The clinical practice of material.Icariin（C33H40O15, molecular weight is 676.67）It is a kind of very effective thin for treating sclerotin
The Chinese medicine ingredients of pine.Many researchs confirm that it can promote the expression of osteoblastic proliferation and Bone formation-related gene, while can
Suppress the activity of osteoclast, be thus advantageous to the healing of sufferers of osteoporosis face osseous tissue.In addition icariin also has and extracts
Process is simple, stable chemical nature, it is easily stored the advantages of.Icariin and calcium phosphate etc. are mixed, biological support is prepared
Material, improves the activity of biomaterial.
Add in collagen-chitin and there is the Chinese medicine preparation for promoting ossification to be comparatively safe method, overcome
The potential untoward reaction of timbering material.So far, there is no collagen-chitin support slow release icariin and form a kind of relative
Safer Alveolar Bone Defect renovating bracket material.
The content of the invention
The technical problem to be solved is the drawbacks described above for overcoming prior art to exist, there is provided a kind of slow release
The collagen-chitin composite of icariin.
Second object of the present invention is to provide the system of the collagen-chitin composite of above-mentioned slow release icariin
Preparation Method.
Third object of the present invention is to provide answering for the collagen-chitin composite of above-mentioned slow release icariin
With.
The purpose of the present invention is achieved by the following technical programs：
A kind of preparation method of the collagen-chitin composite of slow release icariin, comprises the following steps：
S1. 0.336% collagen solution and 2% chitosan solution by volume 5：5～6：4 mixing, in pouring bracket mould into,
After lyophilization, being immersed in successively in the ethanol of variable concentrations carries out gradient deacidification；
S2. add 0.5% genipin to be crosslinked 24h, collagen-chitin timbering material is obtained after cleaning；
S3. the icariin solution of 0.005～0.5mg/mL is uniformly instilled in collagen-chitin timbering material, lyophilization
The collagen-chitin composite of slow release icariin is obtained final product afterwards.
Preferably, gradient deacidification described in S1 be immersed in successively in 100%, 75%, 50%, 25%, 0% ethanol carry out gradient take off
Acid.
Specifically, the time being immersed in 100% ethanol is 2h, and the time being immersed in 75% ethanol is 1.5h, is immersed in
Time in 50% ethanol is 1h, and the time being immersed in 25% ethanol is 30min.
Preferably, 0.336% collagen solution and 2% chitosan solution by volume 5：The timbering material that 5 mixing are obtained
It is void-size distribution uniform.
Preferably, let slip night at -80 DEG C before lyophilization described in S1, cryodesiccated temperature is -30 DEG C, cooling time is
48h。
Preferably, before the collagen-chitin composite of slow release icariin is obtained, repeat step S3；Can
So that timbering material loads more medicines.
The present invention also provides the collagen-chitin composite of the slow release icariin that said method is obtained.
The collagen-chitin composite of slow release icariin of the present invention can be used for Alveolar Bone Defect reparation
Material, therefore the present invention also protects the collagen-chitin composite of slow release icariin repairing as Alveolar Bone Defect
The application of multiple material.
Compared with prior art, the invention has the advantages that：
The invention provides a kind of preparation method of the collagen-chitin composite of slow release icariin, including it is following
Step：S1. 0.336% collagen solution and 2% chitosan solution by volume 5：5～6：4 mixing, in pouring bracket mould into,
After lyophilization, being immersed in successively in the ethanol of variable concentrations carries out gradient deacidification；S2. 0.5% genipin is added to be crosslinked 24h,
Collagen-chitin timbering material is obtained after cleaning；S3. by the icariin solution of 0.005～0.5mg/mL uniformly instill collagen-
In chitosan stent material, the collagen-chitin composite of slow release icariin is obtained final product after lyophilization；The support material
The preparation method is simple of material, material therefor low cost, the collagen-chitin of the slow release icariin that the method is obtained is combined
Material can effectively and for a long time discharge icariin, and timbering material has good controlled drug release, and biological safety is more preferable.
Description of the drawings
Fig. 1 is slow release icariin-collagen-chitin composite preparation flow figure.
Fig. 2 is the collagen-chitin timbering material scanning electron microscope (SEM) photograph that 1% glutaraldehyde cross-linking is obtained；Wherein, Fig. 2A, 2B, 2C
Respectively collagen：Shitosan is 7：Timbering material when 3 amplifies respectively 100/200/500 times；Fig. 2 D, 2E, 2F are respectively collagen：
Shitosan is 6：Timbering material when 4 amplifies respectively 100/200/500 times；Fig. 2 G, 2H, 2I are respectively collagen：Shitosan is 5：5
When timbering material amplify 100/200/500 times respectively.
Fig. 3 is the different collagen-chitin timbering material scanning electron microscope (SEM) photographs of 0.5% genipin crosslinking；Wherein, Fig. 3 A, 3B,
3C is respectively collagen：Shitosan is 7：Timbering material when 3 amplifies respectively 100/200/500 times；Fig. 3 D, 3E, 3F are respectively glue
It is former：Shitosan is 6：Timbering material when 4 amplifies respectively 100/200/500 times；Fig. 3 G, 3H, 3I are respectively collagen：Shitosan is
5：Timbering material when 5 amplifies respectively 100/200/500 times.
Fig. 4 is the collagen-chitin timbering material scanning electron microscope (SEM) photograph of the acquisition of 0.5% genipin crosslinking；Wherein Fig. 4 A, 4B,
4C is respectively collagen：Shitosan is 8：2 timbering material amplifies respectively 100/200/500 times；Fig. 4 D, 4E, 4F are respectively collagen：
Shitosan is 4：6 timbering material amplifies respectively 100/200/500 times.
Fig. 5 is slow release icariin-collagen-chitin compound support frame material cell adhesion result, wherein, Fig. 5 A are do not have
The collagen-chitin support of carrying medicament, has no cell adhesion；Fig. 5 B are the collagen-shell for loading 0.005mg/mL icariin
Polysaccharide support, has no obvious cell adhesion；Fig. 5 C are the collagen-chitin support for loading 0.05mg/mL icariin, it is seen that thin
Born of the same parents adhere to；Fig. 5 D are the collagen-chitin support for loading 0.5mg/mL icariin, it is seen that multiple cell adhesions.
Specific embodiment
Further illustrate present disclosure below in conjunction with Figure of description and specific embodiment, but should not be construed as it is right
The restriction of the present invention.In the case of spirit of the invention and essence, the modification that the inventive method, step, condition are made
Or replace, belong to the scope of the present invention.Unless otherwise noted, experimental technique used in embodiment is people in the art
Conventional method and technology known to member, reagent or material are and are obtained by commercial sources.
The preparation method of the collagen-chitin timbering material of embodiment 1
The method for preparing collagen-chitin timbering material, comprises the steps：
Shitosan is purchased from Guangzhou Qi Yun biotech companies, takes the vinegar that a certain amount of Chitosan powder is dissolved in the 1% of ultra-pure water preparation
In acid solution, blender stirs well even 1h room temperatures（20℃）, prepare 2% chitosan solution.Collagen is purchased from sigma companies, and collagen is molten
Swollen liquid concentration is 0.336%.
0.336% collagen solution and 2% chitosan solution respectively by volume 7：3 uniform mixing, pour bracket mould into
In, after being placed on -80 DEG C overnight, -30 DEG C of freezing 48h in freezer dryer, freezing after-poppet is separately immersed in 100%, 75%, 50%,
25%th, gradient deacidification is carried out in 0% graded ethanol.The deacidification time is deacidification 2h, deacidification 1.5h in 80% ethanol, 50% in 100% ethanol
Deacidification 1h in ethanol, deacidification 30min in 30% ethanol, deacidification 30min in 0% ethanol.After the deacidification step, by collagen-shell
Polysaccharide support is immersed in 24h in cell culture medium, and the pH value of cell culture medium is detected after 24h.It is as effectively de- in do not occurred
Acid, affects cell culture medium pH value, affects cells survival state, or even causes cytotoxicity.
From two kinds of cross-linking agent, 1% glutaraldehyde adds the collagen-chitin support after deacidification to carry out crosslinking 24h, ddH2O is clear
Wash 3 times, each 15min.The surface topography of scanning electron microscopic observation difference cross-linking agents after-poppet material.
The preparation method of the collagen-chitin timbering material of embodiment 2
Experimental technique with embodiment 1, it is unique unlike：0.336% collagen solution and 2% chitosan solution press respectively volume
Than 6：4 uniform mixing.
The preparation method of the collagen-chitin timbering material of embodiment 3
Experimental technique with embodiment 1, it is unique unlike：0.336% collagen solution and 2% chitosan solution press respectively volume
Than 5：5 uniform mixing.
The preparation method of the collagen-chitin timbering material of embodiment 4
Experimental technique with embodiment 1, it is unique unlike：Cross-linking agent is 0.5% genipin.
The preparation method of the collagen-chitin timbering material of embodiment 5
Experimental technique with embodiment 1, it is unique unlike：0.336% collagen solution and 2% chitosan solution press respectively volume
Than 6：4 uniform mixing, and cross-linking agent is 0.5% genipin.
The preparation method of the collagen-chitin timbering material of embodiment 6
Experimental technique with embodiment 1, it is unique unlike：0.336% collagen solution and 2% chitosan solution press respectively volume
Than 5：5 uniform mixing, and cross-linking agent is 0.5% genipin.
Carry out the observation of surface topography to the collagen-chitin material that embodiment 1 to embodiment 6 is obtained first, as a result table
It is bright：Collagen-chitin support loose structure after 1% glutaraldehyde cross-linking is not obvious, and 0.5% genipin crosslinking after-poppet material is in
Loose structure, in addition, early-stage Study result shows：When collagen large percentage（Collagen-chitin ratio is 8：2）, support material
Material hole is larger, and timbering material low intensity, fragility is big.And when shitosan ratio is excessive（Collagen-chitosan ratio is 4：6）,
Frame aperture gap is too small, and timbering material intensity is excessive, and support is not easy to decompose（Fig. 4）, final collagen-chitin ratio is 5：Prop up when 5
Frame pore size distribution is the most uniform, and form is good（Fig. 2, Fig. 3）.
The preparation of the slow release icariin of embodiment 7-collagen-chitin compound support frame material
Icariin（ICA）Standard substance（Purity>99.9%）Purchased from Guangzhou institute for drug control.A certain amount of ICA is weighed, is dissolved in
Dehydrated alcohol/DMSO (DMSO<1% vol%), compound concentration is respectively 0.005mg/mL, 0.05mg/mL and 0.5mg/mL.Configuration
After carry out ultrasonic vibration 30min, ultrasonic vibration frequency is 20KHz, icariin is fully mixed in the solution.
In the collagen-chitin timbering material for adding embodiment 4 to prepare the icariin solution of 0.005mg/mL,
Carry out secondary freeze drying.Comprise the following steps that：The icariin solution of variable concentrations is first configured, ultrasonic vibration 30min makes excessive
Sheep icariin is fully mixed, and uses syringe draw solution, in uniformly dropping in collagen-chitin support.Herba Epimedii will subsequently be immersed
The timbering material of glycosides is placed on ventilation natural drying, and Deca medicine is repeated once after being dried, and after being dried different proportion is obtained
Icariin-collagen-chitin three-dimensional stent material, then carry out secondary freeze drying.
Icariin after preparation-collagen-chitin material is placed in 24 orifice plates, 1mL PBSs, sealing is added
Film closely seals 24 orifice plates, and masking foil covers 24 orifice plate lucifuges, is positioned over 100rpm concussions in 37 DEG C of shaking tables, respectively at 12h,
1d, 3d, 6d take out PBS solution is used for the detection of Icariin content, while adding fresh 1mL PBS.The PBS liquid that will be collected
It is stored in 4 DEG C of refrigerators and keeps in dark place.The sustained concentration of icariin is detected using high performance liquid chromatograph.Concrete steps are such as
Under：The icariin solution of 0.5,1,2.5,5,10 μ g/mL is respectively with hplc grade methanol compound concentration gradient, Qi Feng faces are determined
Product, for making icariin standard curve.The sample of collection is filtered, supernatant is taken and is detected.Testing conditions are
25 DEG C of temperature, flow velocity is 1mL/min, water：Acetonitrile is 65：35, sample-adding amount is 20 μ L, and wavelength 270nm, the time is 8min.According to
Standard curve calculates the content of icariin.
The preparation of the slow release icariin of embodiment 8-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 4 is added to be prepared into the icariin solution of 0.05mg/mL
To collagen-chitin timbering material in.
The preparation of the slow release icariin of embodiment 9-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 4 is added to be prepared into the icariin solution of 0. 5mg/mL
To collagen-chitin timbering material in.
The preparation of the slow release icariin of embodiment 10-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 5 is added to prepare the icariin solution of 0.005mg/mL
In the collagen-chitin timbering material for obtaining.
The preparation of the slow release icariin of embodiment 11-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 5 is added to be prepared into the icariin solution of 0.05mg/mL
To collagen-chitin timbering material in.
The preparation of the slow release icariin of embodiment 12-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 5 is added to be prepared into the icariin solution of 0. 5mg/mL
To collagen-chitin timbering material in.
The preparation of the slow release icariin of embodiment 13-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 6 is added to prepare the icariin solution of 0.005mg/mL
In the collagen-chitin timbering material for obtaining.
The preparation of the slow release icariin of embodiment 14-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 6 is added to be prepared into the icariin solution of 0.05mg/mL
To collagen-chitin timbering material in.
The preparation of the slow release icariin of embodiment 15-collagen-chitin compound support frame material
Experimental technique with embodiment 7, it is unique unlike：Embodiment 6 is added to be prepared into the icariin solution of 0. 5mg/mL
To collagen-chitin timbering material in.
Investigate slow release icariin-collagen-chitin compound support frame material that embodiment 7 is prepared to embodiment 15
Performance, the results are shown in Table 1.
As a result visible collagen-chitin ratio is 7：3,0.005mg/mL, 0.05mg/mL, 0.5mg/mL icariin is dense
When spending, the drug release of 6d is all close to 0 μ g/ mL.Collagen-chitin ratio is 5：5, icariin concentration is 0.5mg/ mL
When more than medicine energy sustained release 6d, and drug release concentration ratio collagen-chitin ratio be 6:It is high when 4.So optimal release
Formula is collagen-chitin ratio 5：5, icariin concentration 0.5mg/ mL.
The adhesive attraction on the slow release icariin of embodiment 16-collagen-chitin compound support frame material surface
Original cuiture Marrow Mesenchymal Stem Cells, slow release icariin-collagen-chitin that embodiment 15 is prepared
24h sterilizations are irradiated under timbering material ultraviolet, is dried.Material faces up and is placed in 6 orifice plates.By cell with containing 0.1% EDTA
0.25% pancreatin digestion, collect, centrifugation, with 2 × 104The density in/hole is seeded on timbering material.After cell culture 7d, will be multiple
Condensation material takes out, and with PBS the cell without adhesion is removed, and the glutaraldehyde room temperatures of composite Jing 3% are fixed into 3h, ddH2O is clear
Wash, then with each 15min of 50%, 70%, 90%, 100% concentration dehydration of alcohol, air-dry overnight, scanning electric mirror observing cell adhesion
Situation, as a result such as Fig. 5, Fig. 5 A are the collagen-chitin support without carrying medicament, have no cell adhesion；Fig. 5 B are load
The collagen-chitin support of 0.005mg/mL icariin, has no obvious cell adhesion；Fig. 5 C are the excessive sheep of load 0.05mg/mL
The collagen-chitin support of icariin, it is seen that cell adhesion；Fig. 5 D are the collagen-chitin for loading 0.5mg/mL icariin
Frame, it is seen that multiple cell adhesions.
Claims (7)
1. it is a kind of it is slow release icariin collagen-chitin composite preparation method, it is characterised in that including following
Step：
S1. 0.336% collagen solution and 2% chitosan solution by volume 5：5～6：4 mixing, in pouring bracket mould into,
After lyophilization, being immersed in successively in the ethanol of variable concentrations carries out gradient deacidification；
S2. add 0.5% genipin to be crosslinked 24h, collagen-chitin timbering material is obtained after cleaning；
S3. the icariin solution of 0.005～0.5mg/mL is uniformly instilled in collagen-chitin timbering material, lyophilization
The collagen-chitin composite of slow release icariin is obtained final product afterwards.
2. the preparation method of the collagen-chitin composite of slow release icariin according to claim 1, it is special
Levy and be, gradient deacidification described in S1 is to be immersed in successively in 100%, 75%, 50%, 25%, 0% ethanol to carry out gradient deacidification.
3. the preparation method of the collagen-chitin composite of slow release icariin according to claim 1, it is special
Levy and be, the time being immersed in 100% ethanol is 2h, the time being immersed in 75% ethanol is 1.5h, in being immersed in 50% ethanol
Time be 1h, the time in 25% ethanol of being immersed in is 30min.
4. the preparation method of the collagen-chitin composite of slow release icariin according to claim 1, it is special
Levy and be, let slip night at -80 DEG C before lyophilization described in S1, cryodesiccated temperature is -30 DEG C, and cooling time is 48h.
5. the preparation method of the collagen-chitin composite of slow release icariin according to claim 1, it is special
Levy and be, before the collagen-chitin composite of slow release icariin is obtained, repeat step S3.
6. any one of claim 1 to 5 preparation method obtain slow release icariin collagen-chitin composite.
7. the collagen-chitin composite of slow release icariin described in claim 6 is as Alveolar Bone Defect reparation
Material application.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109897073A (en) * | 2019-03-21 | 2019-06-18 | 四川大学 | Sulfhydrylation two derivatives from icariin and investigation and its preparation method and application |
| CN115105533A (en) * | 2022-08-16 | 2022-09-27 | 牡丹江医学院 | Medicine for treating gingivitis |
| CN115105533B (en) * | 2022-08-16 | 2024-05-24 | 牡丹江医学院 | Medicine for treating gingivitis |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103495210A (en) * | 2013-09-26 | 2014-01-08 | 福州大学 | Chitosan-hydroxylapatite in-situ loaded icariin composite microspheres |
| CN104368045A (en) * | 2014-11-06 | 2015-02-25 | 四川大学 | Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof |
| CN104436311A (en) * | 2014-11-13 | 2015-03-25 | 吉林医药学院 | Pilose antler polypeptide-collagen-chitosan sustained-release material and preparation method thereof |
| CN104826172A (en) * | 2015-03-06 | 2015-08-12 | 赵红斌 | Scaffold used for bone injury recovery and having tissue inductive function |
| CN105412986A (en) * | 2015-12-14 | 2016-03-23 | 宁波大学 | SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof |
-
2016
- 2016-11-11 CN CN201610994296.2A patent/CN106620878B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103495210A (en) * | 2013-09-26 | 2014-01-08 | 福州大学 | Chitosan-hydroxylapatite in-situ loaded icariin composite microspheres |
| CN104368045A (en) * | 2014-11-06 | 2015-02-25 | 四川大学 | Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof |
| CN104436311A (en) * | 2014-11-13 | 2015-03-25 | 吉林医药学院 | Pilose antler polypeptide-collagen-chitosan sustained-release material and preparation method thereof |
| CN104826172A (en) * | 2015-03-06 | 2015-08-12 | 赵红斌 | Scaffold used for bone injury recovery and having tissue inductive function |
| CN105412986A (en) * | 2015-12-14 | 2016-03-23 | 宁波大学 | SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| CHUN RONG YANG等: "Preparation and biological evaluation of chitosan-collagen-icariin composite scaffolds for neuronal regeneration", 《NEUROL SCI》 * |
| 吴涛等: "仿生组装淫羊藿苷-壳聚糖/羟基磷灰石骨修复材料的体外释药行为", 《中国组织工程研究》 * |
| 唐俊杰等: "骨组织工程诱导性支架材料修复骨缺损", 《中国组织工程研究》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109897073A (en) * | 2019-03-21 | 2019-06-18 | 四川大学 | Sulfhydrylation two derivatives from icariin and investigation and its preparation method and application |
| CN115105533A (en) * | 2022-08-16 | 2022-09-27 | 牡丹江医学院 | Medicine for treating gingivitis |
| CN115105533B (en) * | 2022-08-16 | 2024-05-24 | 牡丹江医学院 | Medicine for treating gingivitis |
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| CN106620878B (en) | 2019-12-31 |
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