CN106609242A - Cell bearing apparatus and cell culture system - Google Patents
Cell bearing apparatus and cell culture system Download PDFInfo
- Publication number
- CN106609242A CN106609242A CN201510699990.7A CN201510699990A CN106609242A CN 106609242 A CN106609242 A CN 106609242A CN 201510699990 A CN201510699990 A CN 201510699990A CN 106609242 A CN106609242 A CN 106609242A
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- China
- Prior art keywords
- cell
- elastica
- bogey
- attached
- culture system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
Abstract
A cell bearing apparatus comprises a piece of elastic membrane, a bearing structure and at least one channel. The elastic membrane is made from polyurethane and used for bearing cells. The bearing structure is fixed at the periphery of the elastic membrane. The at least one channel communicates with the bottom of the elastic membrane and applies stress to the elastic membrane. The invention further provides a cell culture system including the cell bearing apparatus and adherent cells attached to the elastic membrane of the cell bearing apparatus. The cell bearing apparatus is free of extra chemical modification or protein coating, can provide excellent environment for cell growth and cell adherent effect, and is suitable for observation of adherent cells.
Description
Technical field
The present invention relates to a kind of cell bogey, more particularly to a kind of to include a piece of polyurethane
The cell bogey of elastica, a supporting construction and at least one duct, and comprising this
The cell culture system of cell bogey.
Background technology
Because the position that many cells in organism are grown is to be in dynamic environment, for example：The heart,
The organ walls such as lung or stomach can expand with pressure and reduce, and muscle or skin surface can be with pressures
Power and stretch, therefore, when observation in vitro is carried out for cell, need to simulate one and meet
The dynamic environment of organism, with carrying and cultured cells.
It is existing that to provide dynamic environment with the device of observation of cell be to use the resilient silicon of tool
Glued membrane, and external force is applied by machinery or fluid, receive the cell being carried on the pellosil
To dynamic stimulus.However, under life-time service, the pellosil is often because of elastic fatigue
Reduce the robustness of device.
Additionally, observation attached type cell (adherent cell) device do not need only provide for it is good
Cell growth environment, in addition it is also necessary to possess good cell attachment effect, it is thus typically necessary to volume
It is chemically modified outward or protein coating, to promote cell attachment effect.
The content of the invention
It is an object of the invention to provide a kind of cell bogey, it is adaptable to observe attached type thin
Born of the same parents.
The cell bogey of the present invention, comprising a piece of elastica, a supporting construction and at least
One duct, the material of the elastica is polyurethane.The elastica is for carrying cell.Should
Supporting construction is integrally fixed at the elastica periphery.At least one duct is to be communicated to the elastica
Bottom, for applying the elastica is stressed in.
Another object of the present invention is to a kind of cell culture system is provided, comprising as above
Cell bogey, and the attached type cell being attached in the elastica of the cell bogey.
The beneficial effects of the present invention is, the elastic polyurethane film of the cell bogey possesses
Good elastic and durability, and need not additionally be chemically modified or protein coating, you can
Promote cell attachment effect.
Hereinafter will be described in detail with regard to present invention：
It is preferred that the supporting construction is integrally fixed at two opposition sides of the elastica periphery.
In the first embodiment of the present invention, the cell bogey also includes a base,
Every being arranged on the elastica bottom, and at least one duct is through the base.
In the second embodiment of the present invention, the cell bogey also includes a bolster stake,
Be arranged on the elastica bottom, and with the supporting construction interval setting, and the elastica is part
It is attached at the bolster stake.
It is preferred that the polyurethane is polyether polyol is reacted with aliphatic polyisocyante, connect
And react and obtain with chain elongation agent.More preferably, the polyether polyol is selected from Polyethylene Glycol
(PEG), polypropylene glycol (PPG) or its combination.More preferably, the molar ratio of PEG and PPG
For 3：More than 7.The aliphatic polyisocyante can avoid what aromatic compound may have
Risk of toxicity.More preferably, the aliphatic polyisocyante is selected from isophorone diisocyanate
Ester (isophorone diisocyanate, IPDI), hexamethylene diisocyanate (hexamethylene
Diisocyanate, HDI) or its combination.The chain elongation agent is selected from 1,4- butanediols
(1,4-BD), ethylene glycol or its combination.
More preferably, the aliphatic polyisocyante, the polyether polyol and the chain elongation agent rubs
Your proportion is 2：1：1～3：2：1, it is elastic good (percentage elongation is high) and durable (strong to obtain
Degree is high) polyurethane.
According to the cell culture system of the present invention, the attached type cell includes those to be familiar with this
The readily available attached type cell strain of those skilled in the art (is purchased from depositing mechanism in home or overseas),
Or it is separated pure from natural origin using cell isolation method usual in this skill
The attached type cell strain for dissolving.It is preferred that the attached type cell is to be selected to be made up of following
Group：Interstital stem cell (mesenchymal stem cell), fibroblast (fibroblast),
Epithelial cell (epithelial cell), endotheliocyte (endothelial cell), sternzellen
(astrocyte), kidney cell (kidney cell), hepatocyte (hepatocyte), epidermis cell
(epidermal cell), keratocyte (corneal cell), and combinations thereof.More preferably,
The attached type cell is selected from interstital stem cell (placenta-derived derived from Placento-
Mesenchymal stem cell, such as human placenta-derivative interstital stem cell), embryo's kidney
Cell (embryonic kidney cell, such as Human embryonic kidney's cell strain HEK293), embryo
Tire fibroblast (embryonic fibroblast, such as mouse embryo fibroblast cell strain
3T3) or its combination.
Description of the drawings
Other features and effect of the present invention, will be clear in specific embodiment referring to the drawings
Chu ground is presented, wherein：
Fig. 1 is sectional view, illustrates the structure of the cell bogey of first embodiment of the invention；
Fig. 2 is sectional view, and the cell bogey for illustrating the first embodiment is applying vertical side
To vibration stress under structure；
Fig. 3 is sectional view, illustrates the structure of the cell bogey of second embodiment of the invention；
And
Fig. 4 is sectional view, illustrates the cell bogey of the second embodiment in applying level side
To tensile stress under structure.
Specific embodiment
Before the present invention is described in detail, it shall be noted that in the following description content, it is similar to
Element be to be represented with being identically numbered.
The present invention will be described further with regard to following examples, however, it should be noted that the reality
It is only to illustrate use to apply example, and is not necessarily to be construed as the restriction of present invention enforcement.
The preparation ＞ of ＜ elasticas
By IPDI, PEG1000 (weight average molecular weight is 1,000) and PPG1000, (weight is put down
1,000) average molecular weight is with 2：0.5：0.5 molar ratio mixing, at 70 DEG C 2 are reacted
Hour obtain after prepolymer, add Isosorbide-5-Nitrae-BD (as chain elongation agent, its with IPDI mole
Ratio is 1：2), react 24 hours at 110 DEG C, it is molten with the ratio of solid content 20% afterwards
In dimethyl acetylamide (DMAC), and it is coated in a release paper, finally drying is removed
DMAC simultaneously removes the release paper, obtains the elastic polyurethane film that thickness is about 0.025mm.
The test ＞ of ＜ elasticas
Elastic polyurethane film obtained above is carried out into percentage elongation with ASTM D5034
(elongation) test, be as a result 800%, show that elastic polyurethane film obtained above has
At a relatively high percentage elongation.
Elastic polyurethane film obtained above is cultivated human placenta-derivative interstital stem cell
(experimental group), and, at 37 DEG C, 5%CO. with commercially available culture dish as a control group2Environment in
After culture 7 days, the viable cell density (viablecelldensity) measured by experimental group and matched group
Respectively 8.48 × 104/cm2And 5.76 × 104/cm2, the viable cell density of experimental group compared to
Matched group is added significantly to 47%, shows that elastic polyurethane film obtained above can effectively promote
Enter the growth of attached type cell.
＜ cells bogey and cell culture system ＞
Refering to Fig. 1 and Fig. 2, the first embodiment of cell bogey 1 of the present invention is comprising a piece of
The duct 4 of supporting construction 3, four of elastica 2, and a base 5.The elastica 2
To be attached with attached type cell 9 on elastic polyurethane film obtained above, and the elastica 2,
Analog cell is available in the dynamic growth of the organ walls such as meeting frequent expansion and the heart of diminution, lung or stomach
Environment.
The supporting construction 3 is integrally fixed at two opposition sides of the periphery of elastica 2, so that the bullet
Property film 2 is fixed and not moved.
The duct 4 is to be communicated to the bottom of elastica 2, and using an external vacuum equipment
(not shown) is evacuated by the duct 4, so that the elastica 2 carries the attached type
The part of cell 9 be subject to vertical direction stress and to lower recess；When pumping is stopped, the bullet
Property film 2 can be returned to pumping before nonpitting state.By the pressure and frequency that change pumping,
The elastica 2 can be vibrated between etat lacunaire and non-etat lacunaire, so as to the elastica 2
On attached type cell 9 provide vertical direction vibration stress.
The base 5 is disposed on the bottom of elastica 2, and the duct 4 is through the bottom
Seat 5, uniform vertical direction stress is subject to control the elastica 2 in pumping.
Refering to Fig. 3 and Fig. 4, the second embodiment of cell bogey 1 of the present invention is comprising a piece of
The duct 4 of supporting construction 3, of elastica 2, and a bolster stake 6.The elastica 2
To be attached with attached type cell 9 on elastic polyurethane film obtained above, and the elastica 2,
Analog cell is available in the dynamic growth environment of the muscle or skin surface that can frequently stretch.
The supporting construction 3 is integrally fixed at two opposition sides of the periphery of elastica 2, so that the bullet
Property film 2 is fixed and not moved.
The duct 4 is to be communicated to the bottom of elastica 2 and around the bolster stake 6, and utilizes one
External vacuum equipment (not shown) is evacuated by the duct 4, so that the elastica 2 is held
The part for being loaded with the attached type cell 9 is subject to the stress of horizontal direction and flexes outward；Work as stopping
During pumping, the elastica 2 can be returned to the state of non-stretched before pumping.By changing pumping
Pressure and frequency, the elastica 2 can be stretched between extended configuration and non-stretched state, so as to
The tensile stress of horizontal direction is provided to the attached type cell 9 in the elastica 2.
The bolster stake 6 is arranged on the bottom of elastica 2, and by the duct 4 and the support knot
The interval setting of structure 3, and the elastica 2 is that part is attached at the bolster stake 6, to control the patch
Attached part is in pumping by the stress of horizontal direction.
In sum, cell bogey 1 of the present invention is by the good and durable elastica of the elasticity
2, without the need for being additionally chemically modified or protein coating, you can provide good cell growth
Environment and cell attachment effect, with suitable for observation attached type cell 9, so can reach really
The purpose of the present invention.
The above, only embodiments of the invention are of the invention real when being limited with this
The scope applied, i.e., it is all according to claims of the present invention and description made it is simple equivalent
Change and modification, all still belong to the scope of the present invention.
Claims (10)
1. a kind of cell bogey, it is characterised in that it is included：
A piece of elastica, for carrying cell；
One supporting construction, is fixed on the elastica periphery；And
At least one duct, is communicated to the elastica bottom, and for applying the elasticity is stressed in
Film,
Wherein, the material of the elastica is polyurethane.
2. cell bogey according to claim 1, it is characterised in that：This
Support structure is integrally fixed at two opposition sides of the elastica periphery.
3. cell bogey according to claim 2, it is characterised in that：This is thin
Born of the same parents' bogey also includes a base, is disposed on the elastica bottom, and this is at least
One duct is through the base.
4. cell bogey according to claim 2, it is characterised in that：This is thin
Born of the same parents' bogey also includes a bolster stake, is arranged on the elastica bottom, and with the support
Spacing structure is arranged, and the elastica is that part is attached at the bolster stake.
5. cell bogey according to claim 1, it is characterised in that：This gathers
Urethane is polyether polyol is reacted with aliphatic polyisocyante, then anti-with chain elongation agent
Answer and obtain.
6. cell bogey according to claim 5, it is characterised in that：The fat
Fat race polyisocyanates be selected from isophorone diisocyanate, hexamethylene diisocyanate or
Its combination.
7. cell bogey according to claim 5, it is characterised in that：The chain
It is selected from 1,4- butanediols, ethylene glycol or its combination to extend agent.
8. a kind of cell culture system, it is characterised in that it includes according to claim
Cell bogey described in 1, and be attached to attached in the elastica of the cell bogey
Type cell.
9. cell culture system according to claim 8, it is characterised in that：This is attached
The type cell of wearing is selected from by following constituted group：Interstital stem cell, fibroblast,
Epithelial cell, endotheliocyte, sternzellen, kidney cell, hepatocyte, epidermis cell,
Keratocyte, and combinations thereof.
10. cell culture system according to claim 9, it is characterised in that：This is attached
The type cell of wearing is to be selected from interstital stem cell derived from Placento-, Embryonic Kidney cells, embryo's fibre
Dimension blast cell or its combination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510699990.7A CN106609242B (en) | 2015-10-26 | 2015-10-26 | Cell bearing device and cell culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510699990.7A CN106609242B (en) | 2015-10-26 | 2015-10-26 | Cell bearing device and cell culture system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106609242A true CN106609242A (en) | 2017-05-03 |
| CN106609242B CN106609242B (en) | 2020-01-21 |
Family
ID=58612271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510699990.7A Active CN106609242B (en) | 2015-10-26 | 2015-10-26 | Cell bearing device and cell culture system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106609242B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484444A (en) * | 2019-08-15 | 2019-11-22 | 广州迈普再生医学科技股份有限公司 | Biological culture component and biological culture system |
| CN113348240A (en) * | 2019-01-04 | 2021-09-03 | 生物技术食品有限公司 | Bioreactor and method for producing adherent cell culture using the same |
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| CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
| CN101475908A (en) * | 2009-01-16 | 2009-07-08 | 中国人民解放军第三军医大学 | Cultivation apparatus and method for implementing continuous tension stress action to cell |
| CN101842474A (en) * | 2007-08-30 | 2010-09-22 | 哈佛学院董事会 | Compliant surface multi-well culture plate |
| CN102676446A (en) * | 2012-05-04 | 2012-09-19 | 华中科技大学 | Method and device for loading cell fluid stress on deformable curved surface and experimental platform |
| CN103146572A (en) * | 2011-12-07 | 2013-06-12 | 清华大学 | Apparatus and method for realizing homogeneous growth of cell colony |
| CN103477222A (en) * | 2010-09-29 | 2013-12-25 | 麻省理工学院 | Device for high throughput investigations of cellular interactions |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
| CN101842474A (en) * | 2007-08-30 | 2010-09-22 | 哈佛学院董事会 | Compliant surface multi-well culture plate |
| CN101475908A (en) * | 2009-01-16 | 2009-07-08 | 中国人民解放军第三军医大学 | Cultivation apparatus and method for implementing continuous tension stress action to cell |
| CN103477222A (en) * | 2010-09-29 | 2013-12-25 | 麻省理工学院 | Device for high throughput investigations of cellular interactions |
| CN103146572A (en) * | 2011-12-07 | 2013-06-12 | 清华大学 | Apparatus and method for realizing homogeneous growth of cell colony |
| CN102676446A (en) * | 2012-05-04 | 2012-09-19 | 华中科技大学 | Method and device for loading cell fluid stress on deformable curved surface and experimental platform |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113348240A (en) * | 2019-01-04 | 2021-09-03 | 生物技术食品有限公司 | Bioreactor and method for producing adherent cell culture using the same |
| CN110484444A (en) * | 2019-08-15 | 2019-11-22 | 广州迈普再生医学科技股份有限公司 | Biological culture component and biological culture system |
| CN110484444B (en) * | 2019-08-15 | 2022-04-01 | 广州迈普再生医学科技股份有限公司 | Biological culture assembly and biological culture system |
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| Publication number | Publication date |
|---|---|
| CN106609242B (en) | 2020-01-21 |
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