CN106596675A - Temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor - Google Patents
Temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor Download PDFInfo
- Publication number
- CN106596675A CN106596675A CN201611174258.9A CN201611174258A CN106596675A CN 106596675 A CN106596675 A CN 106596675A CN 201611174258 A CN201611174258 A CN 201611174258A CN 106596675 A CN106596675 A CN 106596675A
- Authority
- CN
- China
- Prior art keywords
- hydrogen peroxide
- temperature
- electrode
- controllable
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor. The temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor comprises a gold wire heat electrode, a thiolated single-chain DNA (deoxyribonucleic acid) probe marked with biotin, and streptavidin marked with horse radish peroxidase; one segment of thiolated single-chain DNA marked with the biotin is fixedly arranged at the surface of the gold wire heat electrode and is used as a capturing probe; the biotin and the streptavidin marked on the horse radish peroxidase are specifically bonded; the horse radish peroxidase is fixedly arranged at the surface of the electrode. When the process of horse radish peroxidase enzymatically catalyzing the hydrogen peroxide is electrochemically detected, the temperature of the electrode is adjusted and controlled to improve the enzymatic catalysis activity of the horse radish peroxidase and promote the quicker and more thorough implementing of catalyzing process, thereby realizing the high-sensitivity detection on the hydrogen peroxide. The temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor has the advantages that by adopting the detection method, the detection cost of the hydrogen peroxide is low, the detection sensitivity is high, the detection line is low, and the anti-interference property is good.
Description
Technical field
The invention belongs to analytical chemistry field, and in particular to a kind of temperature-controllable is based on enzymatic electrochemistry hydrogen peroxide
Sensor.
Background technology
Hydrogen peroxide is both a kind of excellent oxidant and a kind of important chemical products, is commonly used as oxidant, disappears
Toxic agent, has a wide range of applications in daily life, food processing and medicine and other fields.During many enzymatic reactions, mistake
Hydrogen oxide is its mesostate, and the metabolic processes internal with many biologies are associated.Because many cells all may be used
To produce hydrogen peroxide, its cross-film transport process is relatively stable, has important adjustment effect to many bioprocesss.So
And, for most of biology, if the content of hydrogen peroxide is too high will to produce toxic action to cell.Hydrogen peroxide
To daily life, environmental pollution and it is healthy and safe etc. have significant impact, therefore research is carried out soon to hydrogen peroxide
Fast, sensitive, accurate detection method has a very big significance.At this stage, the detection method of hydrogen peroxide mainly has light splitting light
Degree method, chemoluminescence method, electrochemical method and chromatography.Wherein electrochemical analysis method, that what is especially wherein studied is most
Be biosensor based on various peroxidase, because of its low cost, operate fairly simple, sensitivity higher and selectivity
Height, receives extensive concern.
Horseradish peroxidase is the complex by iron-protoporphyrin and Glycoprotein binding, main next in nature
Source is Radix Cochleariae officinalises.Horseradish peroxidase property is relatively stable, with special catalytic oxidation performance.
Horseradish peroxidase is a kind of protein very sensitive to temperature change, when temperature is less than optimum temperature,
Enzyme it is active poor, but when temperature is too high, easily there is irreversible degeneration in the structure of enzyme molecule, and cause the mistake of enzyme
It is living.Previously reported current mode Hydrogen Peroxide Biosensor, is that the bulk temperature of Control release system changes mostly, required device
Complexity, operation is difficult；In thermode surface direct construction enzyme sensor, can only change the temperature of electrode surface makes enzyme in most
Suitable condition and overall heating is not carried out to solution, so can improve horseradish peroxidase enzyme catalytic activity so that catalytic reaction
Faster more thoroughly carry out, so that the increase of electrode response signal.
The content of the invention
Present invention aims to prior art is not enough, there is provided a kind of temperature-controllable is based on enzymatic electrochemistry mistake
Oxidation hydrogen sensor.Band of the present invention using the Streptavidin of horseradish peroxidase-labeled with modification on spun gold thermode
There is the sulfhydrylation capture probe specific binding of biotin, Radix Cochleariae officinalises peroxide is turned to into enzyme and is fixed on electrode surface, by regulating and controlling gold
Silk thermode temperature is active to improve horseradish peroxidase enzyme catalytic, realizes the high-sensitivity detection to hydrogen peroxide.This
Bright sensor construction is simple, preparation process is simple, sensitivity is high, test limit is low, anti-interference is good.
To achieve these goals, the present invention is adopted the following technical scheme that：
A kind of preparation method of temperature-controllable based on enzymatic electrochemical hydrogen peroxide detector, specifically includes following steps：
（1）One capture probe DNA of design, the DNA 5 ' holds sulfhydrylation, 3 ' ends to marked biotin；Wherein, capture is visited
The DNA sequence of pin is：5’-HS-(CH2)6-TTTCG TTACT CCCTT CCTCC CCGCA CGGCC-biotin-3’；
（2）By spun gold thermode sanding and polishing into minute surface, Jing redistilled waters are cleaned by ultrasonic, and are dried, the spun gold heat after must processing
Electrode；
（3）Step will be contained（1）3 ' ends of design marked the buffer Deca of the sulfhydrylation capture probe of biotin in step
（2）In modified on the spun gold thermode handled well；Afterwards electric level is immersed with sulfydryl hexanol, electrode surface survivor bits point is carried out
Closing；Again electric level is immersed with bovine serum albumin, electrode active surface site is further closed, obtain the gold of capture probe modification
Silk thermode；
（4）In step（3）In obtain capture probe modification the μ L horseradish peroxidase-labeled of spun gold thermode surface Deca 5
Streptavidin, reaction terminate after obtain electrochemical sensor.
Step（2）In, the polishing of spun gold thermode is on chamois leather and adds alumina powder and is polished；It is cleaned by ultrasonic
Time is 40～60s, and drying is dried up for nitrogen.
Step（3）In, the buffer is 10mM PBS, and its pH is 7.2～7.6.
Step（3）In, it is 1～2 μM that 3 ' ends marked the sulfhydrylation capture probe concentration of biotin；Modification temperature is 37
℃；The modification time is 1.5～2.5h；
Step（3）In, sulfydryl hexanol concentration is 0.1～4mM, and the electrode immersion time is 1h；
Step（3）In, bovine serum albumin concentration is 0.1～2wt%, and the electrode immersion time is 1h；
Step（4）In, the Streptavidin concentration of horseradish peroxidase-labeled is 8～12 μ g/ml, reaction temperature 20～25
DEG C, the response time is 0.2～1h.
Present invention also offers a kind of application of the sensor in detection hydrogen peroxide, detection process is as follows：
Above-mentioned electrochemical sensor and silver-colored silver chloride are participated in into electrode and platinum filament comparison electrode constitutes three-electrode system, removed in logical nitrogen
Voltammetric detection is circulated in the buffer of the hydrogen peroxide of oxygen and 1 mM Pyrogentisinic Acids of addition and variable concentrations, the catalysis for obtaining
Hydrogen peroxide response signal is linear with concentration of hydrogen peroxide, realizes to hydrogen peroxide Sensitive Detection；
In above-mentioned steps, the buffer is 0.1M PBS, and its pH is 7.2～7.6.In electrochemical detection, by regulation and control
Electrode temperature is active to improve horseradish peroxidase enzyme catalytic, promotes catalytic process faster more thoroughly to carry out, and realizes to mistake
The high-sensitivity detection of hydrogen oxide.
The present invention has the advantages that relative to prior art：
（1）Horseradish peroxidase is fixed on electrode by the present invention by the specific binding using Streptavidin biotin
Surface, realizes the high-sensitivity detection to hydrogen peroxide；
（2）In spun gold thermode surface construction biosensor, can only change electrode surface temperature makes Radix Cochleariae officinalises peroxide to the present invention
Compound enzyme does not carry out overall heating in optimal condition to solution, enhances solution convection current, improves mass transfer rate, enhances
Horseradish peroxidase enzyme catalytic activity, promotes the carrying out of catalytic reaction, and increases electrode response signal, improves detection sensitivity
（Test limit reaches 1.3 μM）.
Description of the drawings
Fig. 1 is that a kind of preparation process of the temperature-controllable of the invention based on enzymatic electrochemical hydrogen peroxide detector is illustrated
Figure；
Fig. 2 is the cyclic voltammogram of different modifying electrode；
A is naked spun gold thermode；B is capture probe/spun gold thermode；C is sulfydryl hexanol/capture probe/spun gold thermode；d
For bovine serum albumin/sulfydryl hexanol/capture probe/spun gold thermode；E be horseradish peroxidase/bovine serum albumin/sulfydryl oneself
Alcohol/capture probe/spun gold thermode；
Fig. 3 is experimental condition optimization figure, and catalytic reaction temperature optimizes；
Fig. 4 is electrochemical response of the electrochemical sensor of the present invention to the hydrogen peroxide of variable concentrations；
Fig. 5 is electrochemical response of the electrochemical sensor of the present invention to different detection objects.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, those skilled in the art will manage
Solution, following examples are only the preferred embodiments of the present invention, to more fully understand the present invention, thus should not be regarded as limiting this
Bright scope.
Embodiment 1
Preparation method of a kind of temperature-controllable based on enzymatic electrochemical hydrogen peroxide detector, as shown in figure 1, including following
Step：
（1）One capture probe DNA of design, the DNA 5 ' holds sulfhydrylation, 3 ' ends to marked biotin；Wherein, capture is visited
The DNA sequence of pin is：5’-HS-(CH2)6-TTTCG TTACT CCCTT CCTCC CCGCA CGGCC-biotin-3’；
（2）Spun gold thermode uses 0.05mm Al on chamois leather2O3Minute surface is polished into, with redistilled water 50s is cleaned by ultrasonic；It
Cyclic voltammetric in 0.5M sulfuric acid solutions again afterwards, sweeps fast 0.1V/s and rinses well to secondary water after stably, and nitrogen is dried up；
（3）The 10mM PBS that the pH that 5mL contains the sulfhydrylation capture probe that the ends of 1mM 3 ' marked biotin is 7.4 are buffered molten
Drop is added in step（2）In on the spun gold thermode handled well, time 2h is modified at 37 DEG C；Afterwards with concentration be 2mM sulfydryls oneself
Alcohol is closed to electrode surface survivor bits point, and room temperature lower off-period is 1h, then is 1wt% bovine serum albumins to electricity with concentration
Pole Adsorption point is further closed, and room temperature lower off-period is 1h, obtains the spun gold thermode of capture probe modification；
（4）In step（3）In the L concentration of electrode surface Deca 5 that obtains be 10 μ g/ml horseradish peroxidase-labeled strepto-
Avidin, reacts 0.5h at 25 DEG C, and reaction obtains electrochemical sensor after terminating.
Embodiment 2
Preparation method of a kind of temperature-controllable based on enzymatic electrochemical hydrogen peroxide detector, as shown in figure 1, including following
Step：
（1）One capture probe DNA of design, the DNA 5 ' holds sulfhydrylation, 3 ' ends to marked biotin；Wherein, capture is visited
The DNA sequence of pin is：5’-HS-(CH2)6-TTTCG TTACT CCCTT CCTCC CCGCA CGGCC-biotin-3’；
（2）Spun gold thermode uses 0.05mm Al on chamois leather2O3Minute surface is polished into, with redistilled water 40s is cleaned by ultrasonic；It
Cyclic voltammetric in 0.5M sulfuric acid solutions again afterwards, sweeps fast 0.1V/s and rinses well to secondary water after stably, and nitrogen is dried up；
（3）By the 10mM PBS bufferings that the pH that 5mL contains the sulfhydrylation capture probe that the ends of 1.5mM 3 ' marked biotin is 7.2
Solution Deca is in step（2）In on the spun gold thermode handled well, time 1.5h is modified at 37 DEG C；It is afterwards 0.1mM with concentration
Sulfydryl hexanol is closed to electrode surface survivor bits point, and room temperature lower off-period is 1h, then is 0.1wt% Ox blood serums with concentration
Albumen is further closed to electrode active surface site, and room temperature lower off-period is 1h, obtains the spun gold heat of capture probe modification
Electrode；；
（4）In step（3）In the L concentration of electrode surface Deca 5 that obtains be Radix Cochleariae officinalises peroxide that 8 μ g/ml marked Streptavidin
Enzyme is turned to, 0.2h is reacted at 22 DEG C, reaction obtains electrochemical sensor after terminating.
Embodiment 3
Preparation method of a kind of temperature-controllable based on enzymatic electrochemical hydrogen peroxide detector, as shown in figure 1, including following
Step：
（1）One capture probe DNA of design, the DNA 5 ' holds sulfhydrylation, 3 ' ends to marked biotin；Wherein, capture is visited
The DNA sequence of pin is：5’-HS-(CH2)6-TTTCG TTACT CCCTT CCTCC CCGCA CGGCC-biotin-3’；
（2）Spun gold thermode uses 0.05mm Al on chamois leather2O3Minute surface is polished into, with redistilled water 60s is cleaned by ultrasonic；It
Cyclic voltammetric in 0.5M sulfuric acid solutions again afterwards, sweeps fast 0.1V/s and rinses well to secondary water after stably, and nitrogen is dried up；
（3）The 10mM PBS that the pH that 5mL contains the sulfhydrylation capture probe that the ends of 2mM 3 ' marked biotin is 7.6 are buffered molten
Drop is added in step（2）In on the spun gold thermode handled well, time 2.5h is modified at 37 DEG C；It is afterwards 4mM sulfydryls with concentration
Hexanol is closed to electrode surface survivor bits point, and room temperature lower off-period is 1h, then is 2wt% bovine serum albumins pair with concentration
Electrode active surface site is further closed, and room temperature lower off-period is 1h, obtains the spun gold thermode of capture probe modification；；
（4）In step（3）In the L concentration of electrode surface Deca 5 that obtains be Radix Cochleariae officinalises mistake that 12 μ g/ml marked Streptavidin
Enzyme is oxidized to, 1h is reacted at 20 DEG C, reaction obtains electrochemical sensor after terminating.
Electrochemical Detection：
Electrochemical sensor obtained in embodiment 1 and silver-colored silver chloride are participated in into electrode and platinum filament comparison electrode constitutes three electrode systems
System, is that the PBS that 7.4 concentration are 0.1M delays in the pH of letting nitrogen in and deoxidizing and the hydrogen peroxide for adding 1 mM Pyrogentisinic Acids and variable concentrations
Rush in liquid and be circulated Voltammetric detection, in electrochemical detection, by regulating and controlling electrode temperature horseradish peroxidase is improved
Catalysis activity, the electrochemical catalysis hydrogen peroxide response signal for obtaining is linear with concentration of hydrogen peroxide, realizes to peroxide
Change hydrogen Sensitive Detection.
Electrochemical detection electrode temperature is arranged to, from 0～55 DEG C, to be tested respectively successively, other reaction conditions
It is constant；The cyclic voltammetric peak current for obtaining is mapped to temperature, as shown in figure 3, electrode temperature from be increased to 40 DEG C for 0 DEG C when, also
Parent peak electric current is significantly increased, to 40 DEG C again toward rise to 55 DEG C when, reduction peak current reduce, illustrate that electrode temperature is raised to 40 DEG C
When, the catalysis activity of horseradish peroxidase is maximum.Therefore this experiment selects 40 C as Optimal Temperature, under Optimal Temperature
To realize the highly sensitive detection of hydrogen peroxide.
Electrochemical response characteristic of the electrochemical sensor of the present invention to the hydrogen peroxide of variable concentrations：
Only change concentration of hydrogen peroxide in the liquid of Electrochemical Detection bottom, tested respectively successively, other reaction conditions are constant；As schemed
Shown in 4, the cyclic voltammetric peak current for obtaining is made into equation of linear regression to concentration of hydrogen peroxide, obtain working curve.
Its testing result as shown in figure 4, when electrochemical detection electrode temperature is 0 C, its response current value and mistake
Hydrogen peroxide concentration is 5.0 × 10-5 ~ 1.2 × 10-3 mol L–1Linearly, linear equation is：I (μA) = 0.61215
× c (mM)+0.04335, R2 = 0.9986；Detection is limited to 1.5 × 10–5 mol L–1.When electrode is warming up to 40 C,
Response current value is with concentration of hydrogen peroxide 3.0 × 10–6~4×10–4 mol L–1In the range of it is linear, linear equation is：
I (μ A)=1.63893 × c (mM)+0.03525, R2= 0.98589；Detection is limited to 1.3 × 10–6 mol L–1.Phase
Than in 0 C, its test limit reduces 1 order of magnitude, show a liter high-temperature, the catalysis activity of horseradish peroxidase strengthens, real
The highly sensitive detection to hydrogen peroxide is showed.
Electrochemical response characteristic of the electrochemical sensor of the present invention to different detection objects：
Only change Electrochemical Detection object, respectively：Blank solution, 0.05 mM hydrogen peroxide, 0.05 mM hydrogen peroxide
With 0.5 mM citric acid mixed liquors, 0.05 mM hydrogen peroxide and 0.5 mM glucose mixed liquors, 0.05 mM hydrogen peroxide and
0.5 mM sucrose mixed liquors, 0.05 mM hydrogen peroxide and 0.5 mM TYR mixed liquors.Tested respectively successively, other are anti-
Answer condition constant；As shown in figure 5, dotted line is blank solution, other five kinds of bottom curve liquid overlap, and say the material of electricity addition to electrode
Without impact, the bright anti-interference of electrochemical sensor is good.
Claims (10)
1. a kind of temperature-controllable is based on the preparation method of enzymatic electrochemical hydrogen peroxide detector, it is characterised in that：Including
Following steps：
（1）One capture probe DNA of design, the DNA 5 ' holds sulfhydrylation, 3 ' ends to marked biotin；Wherein, capture is visited
The DNA sequence of pin is：5’-HS-(CH2)6-TTTCG TTACT CCCTT CCTCC CCGCA CGGCC-biotin-3’；
（2）By spun gold thermode sanding and polishing into minute surface, Jing redistilled waters are cleaned by ultrasonic, and are dried, the spun gold heat after must processing
Electrode；
（3）Step will be contained（1）3 ' ends of design marked the buffer Deca of the sulfhydrylation capture probe of biotin in step
（2）In modified on the spun gold thermode handled well；Afterwards electric level is immersed with sulfydryl hexanol, electrode surface survivor bits point is carried out
Closing；Again electric level is immersed with bovine serum albumin, electrode active surface site is further closed, obtain the gold of capture probe modification
Silk thermode；
（4）In step（3）In obtain capture probe modification the μ L horseradish peroxidase-labeled of spun gold thermode surface Deca 5
Streptavidin, reaction terminate after obtain electrochemical sensor.
2. a kind of temperature-controllable as claimed in claim 1 is based on the preparation side of enzymatic electrochemical hydrogen peroxide detector
Method, it is characterised in that：Step（2）In, the polishing of spun gold thermode is on chamois leather and adds alumina powder and is polished；Ultrasound
The time of cleaning is 20～60s, and drying is dried up for nitrogen.
3. a kind of temperature-controllable as claimed in claim 1 is based on the preparation side of enzymatic electrochemical hydrogen peroxide detector
Method, it is characterised in that：Step（3）In, the buffer is 10mM PBS, and its pH is 7.2～7.6.
4. a kind of temperature-controllable as claimed in claim 1 is based on the preparation side of enzymatic electrochemical hydrogen peroxide detector
Method, it is characterised in that：Step（3）In, it is 1～2 μM that 3 ' ends marked the sulfhydrylation capture probe concentration of biotin；Modification temperature
For 37 DEG C；The modification time is 1.5～2.5h.
5. a kind of temperature-controllable as claimed in claim 1 is based on the preparation side of enzymatic electrochemical hydrogen peroxide detector
Method, it is characterised in that：Step（3）In, sulfydryl hexanol concentration is 0.1～4mM, and the electrode immersion time is 1h；Bovine serum albumin is dense
Spend for 0.1～2wt%, the electrode immersion time is 1h.
6. a kind of temperature-controllable as claimed in claim 1 is based on the preparation side of enzymatic electrochemical hydrogen peroxide detector
Method, it is characterised in that：Step（4）In, the concentration of the Streptavidin of horseradish peroxidase-labeled is 8～12 μ g/ml, instead
20～25 DEG C of temperature is answered, the response time is 0.2～1h.
7. a kind of temperature-controllable that prepared by the method as described in any one of claim 1-6 is based on enzymatic electrochemistry peroxidating
Hydrogen sensor.
8. application of the electrochemical sensor as described in right 7 in detection hydrogen peroxide, it is characterised in that：Detection process is such as
Under：
The electrochemical sensor and silver-colored silver chloride are participated in into electrode and platinum filament comparison electrode constitutes three-electrode system, removed in logical nitrogen
Voltammetric detection is circulated in the buffer of the hydrogen peroxide of oxygen and 1 mM Pyrogentisinic Acids of addition and variable concentrations, with hydroquinone
For electron mediator, the electro-catalysis hydrogen peroxide response signal for obtaining is linear with concentration of hydrogen peroxide, realizes to peroxidating
Hydrogen Sensitive Detection.
9. application as claimed in claim 8, it is characterised in that：The buffer is 0.1M PBS, and its pH is 7.2～7.6.
10. application as claimed in claim 8, it is characterised in that：In electrochemical detection, carried by regulating and controlling electrode temperature
High horseradish peroxidase catalysis activity, promotes catalytic process faster more thoroughly to carry out, and realizes the Gao Ling to hydrogen peroxide
Sensitivity is detected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611174258.9A CN106596675A (en) | 2016-12-19 | 2016-12-19 | Temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611174258.9A CN106596675A (en) | 2016-12-19 | 2016-12-19 | Temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106596675A true CN106596675A (en) | 2017-04-26 |
Family
ID=58601300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611174258.9A Pending CN106596675A (en) | 2016-12-19 | 2016-12-19 | Temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106596675A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107144619A (en) * | 2017-06-21 | 2017-09-08 | 福州大学 | The electrochemical DNA biosensor of a kind of temperature-controllable based on enzymatic and preparation method thereof |
| CN107228892A (en) * | 2017-05-19 | 2017-10-03 | 福州大学 | Electrochemistry mercury ion sensor of temperature-controllable and preparation method thereof |
| CN110484601A (en) * | 2019-08-31 | 2019-11-22 | 福州大学 | Electrochemistry p53 gene sensor of temperature-controllable and its preparation method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063715A (en) * | 2012-11-03 | 2013-04-24 | 福建医科大学 | Method for detecting surviving gene based on graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor |
| CN103175873A (en) * | 2013-01-27 | 2013-06-26 | 福州市第二医院 | DNA electrochemical sensor based on target DNA repetitive sequence self enhancement and amplification signal |
| CN104614429A (en) * | 2015-02-09 | 2015-05-13 | 福州大学 | Manufacture of gold plated copper thermode and application of thermode on temperature-controllable H2O2 sensor |
-
2016
- 2016-12-19 CN CN201611174258.9A patent/CN106596675A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063715A (en) * | 2012-11-03 | 2013-04-24 | 福建医科大学 | Method for detecting surviving gene based on graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor |
| CN103175873A (en) * | 2013-01-27 | 2013-06-26 | 福州市第二医院 | DNA electrochemical sensor based on target DNA repetitive sequence self enhancement and amplification signal |
| CN104614429A (en) * | 2015-02-09 | 2015-05-13 | 福州大学 | Manufacture of gold plated copper thermode and application of thermode on temperature-controllable H2O2 sensor |
Non-Patent Citations (1)
| Title |
|---|
| CAN ZHANG ET AL.: "Endonuclease cleavage combined with horseradish peroxidase-assisted signal amplification for electrochemical monitoring of DNA", 《ELECTROCHEMISTRY COMMUNICATIONS》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107228892A (en) * | 2017-05-19 | 2017-10-03 | 福州大学 | Electrochemistry mercury ion sensor of temperature-controllable and preparation method thereof |
| CN107228892B (en) * | 2017-05-19 | 2019-08-09 | 福州大学 | Electrochemistry mercury ion sensor of temperature-controllable and preparation method thereof |
| CN107144619A (en) * | 2017-06-21 | 2017-09-08 | 福州大学 | The electrochemical DNA biosensor of a kind of temperature-controllable based on enzymatic and preparation method thereof |
| CN110484601A (en) * | 2019-08-31 | 2019-11-22 | 福州大学 | Electrochemistry p53 gene sensor of temperature-controllable and its preparation method and application |
| CN110484601B (en) * | 2019-08-31 | 2022-12-09 | 福州大学 | Temperature-controllable electrochemical p53 gene sensor and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10605761B2 (en) | Electrochemical biosensor based on aptamer/nano silver probe and EXO I enzyme | |
| Karadag et al. | Modified gold surfaces by 6-(ferrocenyl) hexanethiol/dendrimer/gold nanoparticles as a platform for the mediated biosensing applications | |
| US5653862A (en) | Biochemical sensor device and method | |
| CN105784796B (en) | A kind of sensitive determination method of the aptamer sensor based on gold/molybdenum disulfide/graphene nanocomposite material to lysozyme | |
| CN106596675A (en) | Temperature-controllable enzymatic catalysis-based type electrochemical hydrogen peroxide sensor | |
| CN101140257B (en) | Biologic sensor enzyme functional susceptivity film containing nickel and aluminum hydrotalcite nano piece and method of producing the same | |
| Milardović et al. | Glucose determination in blood samples using flow injection analysis and an amperometric biosensor based on glucose oxidase immobilized on hexacyanoferrate modified nickel electrode | |
| Zhu et al. | Fabrication of magneto-controlled moveable architecture to develop reusable electrochemical biosensors | |
| CN106568820B (en) | The preparation method and applications of the electrochemica biological sensor of silver nanoclusters are synthesized based on DNA signal amplification techniques | |
| CN108051492B (en) | A method of based on controllable self assembly forceps constructions Electrochemical Detection single-stranded target DNA | |
| CN104764784A (en) | Biosensor for detection of mercury ions based on aptamer and preparation method thereof | |
| CN111007122A (en) | Three-dimensional flower-like nano composite material and preparation method and application thereof | |
| CN109856206B (en) | Method for detecting concentration of dopamine | |
| CN102654475B (en) | Bioelectrochemical sensor used for detecting hydrogen peroxide and manufacturing method thereof | |
| CN105044179A (en) | Three-dimensional graphene-modified electrode used for detecting tumor markers and preparation method of three-dimensional graphene-modified electrode | |
| Ikariyama et al. | High performance micro-enzyme sensor using platinized microelectrode. | |
| CN105403606A (en) | Preparation method for carbon cloth electrode based on cobalt phosphide/hemoglobin modification | |
| CN107144619B (en) | Temperature-controllable enzyme catalysis-based electrochemical DNA sensor and preparation method thereof | |
| JP4654411B2 (en) | Hydrogen peroxide concentration measurement method, enzyme activity measurement method and immunochemical measurement method | |
| Zhang et al. | Miniaturised electrochemical analyser for glucose determination based on Chitosan/GOD/Electroreduced graphene oxide sensor | |
| Li et al. | Amperometric nonenzymatic determination of glucose free of interference based on poly (sulfosalicylic acid) modified nickel microelectrode | |
| CN114019004B (en) | Electrochemical detection method of gram-negative bacteria, sensor and preparation method of sensor | |
| JPH04279854A (en) | Platinum coated carbon fiber electrode and enzymatic film sensor using same | |
| Rad et al. | A new electrochemical biosensor for hydrogen peroxide using HRP/AgNPs/cysteamine/p-ABSA/GCE self-assembly modified electrode | |
| CN102539480A (en) | Preparation method of amperometric glucose sensor based on highly-ordered titanium dioxide nanotube array |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170426 |