CN106596607A - Diffractometer capable of measuring protein crystal data at high precision - Google Patents
Diffractometer capable of measuring protein crystal data at high precision Download PDFInfo
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- CN106596607A CN106596607A CN201611249140.8A CN201611249140A CN106596607A CN 106596607 A CN106596607 A CN 106596607A CN 201611249140 A CN201611249140 A CN 201611249140A CN 106596607 A CN106596607 A CN 106596607A
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- diffractometer
- induction pieces
- diffractometer according
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- sensor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/20—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials
- G01N23/20008—Constructional details of analysers, e.g. characterised by X-ray source, detector or optical system; Accessories therefor; Preparing specimens therefor
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- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Abstract
The invention discloses a diffractometer capable of measuring protein crystal data at high precision. The diffractometer comprises an X-ray generation device, an X-ray detection device and a control device, wherein the X-ray detection device comprises a measuring head provided with a sample. The diffractometer is characterized in that a pressure sensing unit used for sensing changes of pressure applied to a to-be-measured sample is arranged on the measuring head. The stability of the diffractometer can be quantificationally monitored, influence of the stability of the diffractometer on sample analysis data is eliminated, and the diffractometer is simple in structure and high in sensitivity and more accurately measures data.
Description
Technical field
The invention belongs to X-ray diffractometer field, can accurately measure albumin crystal data more particularly, to a kind of
Diffractometer.
Background technology
X-ray diffraction measurement is to identify the important means of biomacromolecule phase structure.Generally high-precision experimental data is obtained
The diffractometer of the light source, high-quality albumin crystal sample and high stability of high stability must be needed, if there is diffraction data
Undesirable situation, it may be possible to which light there occurs shake, it may be possible to which albumin crystal sample property is not good, it is also possible to be sample
There occurs and rock, sample is rocked it could also be possible that effect of jitter its stability for causing diffractometer is rocked on ground.In order to arrange
Except the impact that the unstability of diffractometer is brought to diffraction data, the unstability of quantitative observation diffractometer, judgement sample are needed
Situation on diffractometer, it is undesirable to attempt solving the data brought of diffractometer stability.And current diffractometer on the market
Lack the Method and kit for of quantitative its stability of monitor in real time.
The content of the invention
In order to overcome the deficiencies in the prior art, the present invention to provide a kind of introducing and carry out real-time quantitative to the stability of diffractometer
Monitoring, measurement data more accurately can accurately measure the diffractometer of albumin crystal data.
The technical solution adopted for the present invention to solve the technical problems is：One kind can accurately measure albumin crystal data
Diffractometer, including X-ray generator, X-ray detection device and control device, the X-ray detection device include installing
There is the Angle measurement of sample, the Angle measurement is provided with the pressure sensing list of the pressure change put on for perception on testing sample
Unit.The stability of sample can quantitatively be monitored by the setting of pressure sensitive unit, and then Quantitative Monitoring diffractometer is stablized
Property, exclude the unstable impact brought to diffraction patterns of diffractometer so that more accurate to the data analysiss of albumin crystal.
Further, the pressure sensitive unit is included the connecting seat that can be connected with detection device, treats test sample for placing
The placement portion of product, for sensing deformation produced by the change of placement portion upward pressure and deformation be converted into the inductive component of the signal of telecommunication
And for converting the electrical signal to the display device of digital signal.
Further, the inductive component includes the induction pieces, sensor on induction pieces, upper with what placement portion was connected
Connector and the lower connector being connected with connecting seat.
Further, the induction pieces are provided with for lifting the thinner wall section of its adaptability to changes.The setting of thinner wall section causes sense
Answering the strain property of part more preferably, larger deformation also being produced when by less power, sensitivity is higher, and final data is more
Precisely.
Further, the sensor is resistance strain gage.
Further, positioned at sensor just to position, thinner wall section is provided with and sets through induction pieces side wall the thinner wall section
The through hole put.
Further, the through hole is made up of the round hole that three horizontal cross are overlapped, wherein middle circle
Round hole area of the perforated area less than both sides.The ingenious setting of three round holes allows induction pieces preferably to sense
Stress changes, and after the thickness of induction pieces is reduced, pressure varies slightly just to pass through the present display device of sensor body well
On, and the superposition of round hole susceptiveness for other shapes is more preferable, pressure slight change just passes through round hole side
The distortion of edge allows sensor to embody more rapidly, more accurately deformation quantity.
Further, be provided with the first pad between the upper connector and induction pieces, the induction pieces and lower connector it
Between be provided with the second pad, first pad and the second pad cross-sectional area it is equal.The shape of the first pad and the second pad
Size is identical so that the lifting surface area of induction pieces is concentrated on the first pad and the second pad such that it is able to which preferably sensing is applied
The pressure size of induction pieces both sides is added in, and pressure size is embodied a concentrated reflection of on induction pieces, display is sent to by sensor
The data of device are more accurate.
Further, the upper connector is provided with the storage tank for being offset for induction pieces within the specific limits.It is prevented effectively from
Induction pieces are contacted with upper connector causes the inaccurate of test data.
Further, the upper connector is provided with for housing the accommodating chamber of sensor.Upper connector is avoided with sensing
Part produces the contact surface thought except the first pad.
The invention has the beneficial effects as follows：Traditional Thinking is broken through, increases a dimension to the analysis of diffraction image, in diffractometer
Detection device on be provided for perceiving the pressure sensitive unit of sample stability, and then quantitatively monitor diffractometer and stablize
Property, impact of the stability of diffractometer to sample analysis data is excluded, simple structure, sensitivity are high, and measurement data is more accurate.
Description of the drawings
Fig. 1 is the structural representation one of pressure sensitive unit in the present invention.
Fig. 2 is the structural representation two of pressure sensitive unit in the present invention.
Fig. 3 is the structural representation three of pressure sensitive unit in the present invention.
Fig. 4 is the fit structure schematic diagram of lower connector and induction pieces.
Stressing conditions schematic diagrams of the Fig. 5 for induction pieces.
Fig. 6 is the fit structure schematic diagram of the part-structure with light source of the present invention.
Part-structure rough schematics of the Fig. 7 for diffractometer.
Specific embodiment
In order that those skilled in the art are better understood from the present invention program, below in conjunction with the embodiment of the present invention
Accompanying drawing, carries out clear, complete description to the technical scheme in inventive embodiments, it is clear that described embodiment is only this
A part of embodiment of invention, rather than the embodiment of whole.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, should all belong to the scope of protection of the invention.
Shown in reference picture 1-7, a kind of diffractometer that can accurately measure albumin crystal data, including X-ray generation dress
4, control device and X-ray detection device 5 are put, X-ray detection device 5 includes the Angle measurement 51 for being provided with sample, Angle measurement 51
It is provided with pressure sensitive unit.Pressure sensitive unit includes the connecting seat 1 being connected with X-ray detection device successively, for putting
The placement portion 3 of testing protein crystal prototype is put, is changed produced deformation and deformation is changed for sensing 3 upward pressure of placement portion
Into the inductive component of the signal of telecommunication and for converting the electrical signal to digital signal and being displayed in the display device on screen.
Placement portion 3 includes needle tubing 31 for adhesion testing protein crystal prototype, needle tubing 31 is adsorbed company by magnetic part
The upper segment body 32 for connecing and the lower segment body 34 being detachably connected on by knob 33 on upper connector 23.Knob 33 is provided with interior spiral shell
Stricture of vagina, upper connector 23 are provided with the projection being adapted to knob 33, and projection is provided with external screw thread, first places lower segment body 34 during assembling
Above projection, knob 33 is set in rotation threaded connection on the outside of projection through lower segment body 34.In order to mitigate overall weight, peace
Put portion 3 to be made up of aluminum, improve overall sensitivity.Upper segment body 32 can be hollow structure, on the side of lower segment body 34
Offer the groove 341 passed through for optical fiber.
Inductive component is included the lower connector 24 being connected with connecting seat 1, the induction pieces 21 being connected with lower connector 24, is arranged
In the sensor 22 on induction pieces 21 and the upper connector 23 being arranged on above induction pieces 21.Induction pieces 21 are in rectangular structure,
Sensor 22 is fixedly connected with induction pieces 21, is offered on the upper connector 23 in cylinder for housing the appearance of sensor 22
Receive chamber 232, specifically, accommodating chamber 232 is the circular groove for being opened in 23 lower surface of upper connector, the center of circle of the circular groove with
The center of upper connector 23 essentially coincides.In the present embodiment, sensor 22 includes resistance strain gage and for by resistance-strain
Piece is fixedly connected on the silica gel on induction pieces 21, and the upper surface of induction pieces 21 is centrally through the connected resistance strain gage of silica gel, resistance
The lead of foil gauge is connected with display device by wire 221.In order to improve the adaptability to changes of sensor, induction pieces 21 and sensing
Device 22 is just provided with thinner wall section to position, specifically, opens up through hole 211 in the center position of induction pieces 21, and through hole 211 is passed through
The side wall for wearing induction pieces 21 is arranged, and through hole 211 is made up of three round holes on same straight line, three round holes
Juxtaposition is arranged, and the round hole cross-sectional area positioned at both sides is equal and more than positioned at middle round hole cross-sectional area.
The left end of 21 upper surface of induction pieces is contacted with upper connector 23 by the first pad 251 and is connected, induction pieces
The right-hand end of 21 lower surfaces is contacted with lower connector 24 by the second pad 252 and is connected, in order to ensure 23 He of upper connector
The conduction surface that lower connector 24 is applied to the power on induction pieces 21 is equal, the cross section of the first pad 251 and the second pad 252
Shape, size are identical, i.e. the lifting surface area of induction pieces both sides about 21 is equal.In order to avoid the right-hand end of 21 upper surface of induction pieces
Portion is contacted with upper connector 23, forms a storage tank 231 at the lower surface edge of upper connector 23, even if so as to induction pieces 21
Shift within the specific limits, also can guarantee that the right side upper surface of induction pieces 21 will not contact upper connector 23, to induction pieces
21 deformation will not produce impact, and final data measurement is more accurate.Kidney-shaped structure of the lower connector 24 in bending, which passes through to pass through
The installing hole for putting on lower wall is connected on connecting seat 1, and X-ray detection device other are supplied at the intermediate recess of lower connector 24
Part passes through.
Display device（Not shown in figure）It is electrically connected by wire 221 and inductive component, display device will be produced on induction pieces 21
Raw deformation is converted into the signal of telecommunication by sensor, and the signal of telecommunication is converted into digital signal by converter, and digital signal can lead to
Cross display screen to show, be easy to intuitively read the pressure being applied in placement portion 3.Digital signal passes through human-computer interaction interface simultaneously
Show in real time, all data storages on computers, are called after being easy to.
The present invention operation principle be：Pressure sensitive unit is connected to into the angle measurement of X-ray detection device by connecting seat
Head, as action of gravity, placement portion and upper connector produce level power to the right, lower company by the first pad to induction pieces
Fitting produces level power to the left by the second pad to induction pieces, and induction pieces produce certain deformation and are embodied in display dress
Put, original state is reset to display device, when testing sample is placed in placement portion, due to being applied to the first pad and second
Power on pad changes, and induction pieces produce more deformation, and deformation is converted into the signal of telecommunication and passes through wire transmission extremely by sensor
Display device, display device convert electrical signals into digital signal, and experimenter conveniently, intuitively can be read on inductive component
Pressure change data.
When sample to be placed placement portion, the instantaneous pressure value read in display device relative to original state occur compared with
Big to change, when sample is removed placement portion, there is larger changing relative to original state in the instantaneous pressure value read in display device
Become, when being analyzed to sample, the pressure value read in display device is in more stable state, under normal condition, will not produce
The larger fluctuation of life.
If it was found that diffraction image is not good, transfer the data stored on computer, if it find that pressure value except setting-out and
There is fluctuation during sampling, be constantly in more stable state, illustrate the stability of sample preferably, further relate to stablizing for diffractometer
Property preferably, eliminate the problem of diffractometer；If data display diffraction image is not good, pressure value also produces larger ripple
It is dynamic, illustrate that the stability of sample is not good, it may be possible to the de-stabilising effect of diffractometer diffraction image, asking for light source etc. can be excluded
Topic causes diffraction image not good.Such that it is able to judge associating between the stability of diffractometer and sample diffraction image.
Above-mentioned specific embodiment is used for illustrating the present invention, rather than limits the invention, the present invention's
In spirit and scope of the claims, any modifications and changes made to the present invention both fall within the protection model of the present invention
Enclose.
Claims (10)
1. a kind of diffractometer that can accurately measure albumin crystal data, including X-ray generator (4), X-ray detection dress
(5) and control device are put, the X-ray detection device (5) includes the Angle measurement (51) for being provided with sample, it is characterised in that：Institute
State the pressure sensitive unit that Angle measurement (51) is provided with the pressure change put on for perception on testing sample.
2. diffractometer according to claim 1, it is characterised in that：The pressure sensitive unit includes can be with detection device phase
Connecting seat (1) even, the placement portion (3) for placing testing sample, for sensing produced by the change of placement portion (3) upward pressure
Deformation is converted into the inductive component and display device for converting the electrical signal to digital signal of the signal of telecommunication simultaneously for deformation.
3. diffractometer according to claim 2, it is characterised in that：The inductive component includes induction pieces (21), located at sense
Answer the sensor (22) on part (21), the upper connector (23) being connected with placement portion (3) and the lower connection being connected with connecting seat (1)
Part (24).
4. diffractometer according to claim 3, it is characterised in that：The induction pieces (21) are provided with for lifting its strain
The thinner wall section of power.
5. diffractometer according to claim 3, it is characterised in that：The sensor (22) is resistance strain gage.
6. diffractometer according to claim 4, it is characterised in that：The thinner wall section is located at the positive para-position of sensor (22)
Put, thinner wall section is provided with the through hole (211) arranged through induction pieces (21) side wall.
7. diffractometer according to claim 6, it is characterised in that：The through hole (211) is overlapped by three horizontal cross and is set
The round hole composition put, wherein round hole area of the middle round hole area less than both sides.
8. diffractometer according to claim 2, it is characterised in that：Set between the upper connector (23) and induction pieces (21)
There is the first pad (251), between the induction pieces (21) and lower connector (24), be provided with the second pad (252), first pad
The cross-sectional area of piece (251) and the second pad (252) is equal.
9. diffractometer according to claim 8, it is characterised in that：The upper connector (23) is provided with for induction pieces (21)
The storage tank (231) for offseting within the specific limits.
10. diffractometer according to claim 8, it is characterised in that：The upper connector (23) is provided with for accommodating biography
The accommodating chamber (232) of sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611249140.8A CN106596607B (en) | 2016-12-29 | 2016-12-29 | A kind of diffractometer of energy accurately measure albumin crystal data |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611249140.8A CN106596607B (en) | 2016-12-29 | 2016-12-29 | A kind of diffractometer of energy accurately measure albumin crystal data |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106596607A true CN106596607A (en) | 2017-04-26 |
| CN106596607B CN106596607B (en) | 2019-02-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611249140.8A Active CN106596607B (en) | 2016-12-29 | 2016-12-29 | A kind of diffractometer of energy accurately measure albumin crystal data |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106596607B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0875678A (en) * | 1994-09-01 | 1996-03-22 | Mac Sci:Kk | Quadruple shaft-type automatic diffraction apparatus |
| US7796726B1 (en) * | 2006-02-14 | 2010-09-14 | University Of Maryland, Baltimore County | Instrument and method for X-ray diffraction, fluorescence, and crystal texture analysis without sample preparation |
| CN102435626A (en) * | 2011-09-13 | 2012-05-02 | 丹东通达科技有限公司 | Table type X-ray diffractometer |
| CN205229075U (en) * | 2015-11-12 | 2016-05-11 | 中国科学院上海应用物理研究所 | Response recognition device of protein crystal sample acquisition loop base |
-
2016
- 2016-12-29 CN CN201611249140.8A patent/CN106596607B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0875678A (en) * | 1994-09-01 | 1996-03-22 | Mac Sci:Kk | Quadruple shaft-type automatic diffraction apparatus |
| US7796726B1 (en) * | 2006-02-14 | 2010-09-14 | University Of Maryland, Baltimore County | Instrument and method for X-ray diffraction, fluorescence, and crystal texture analysis without sample preparation |
| CN102435626A (en) * | 2011-09-13 | 2012-05-02 | 丹东通达科技有限公司 | Table type X-ray diffractometer |
| CN205229075U (en) * | 2015-11-12 | 2016-05-11 | 中国科学院上海应用物理研究所 | Response recognition device of protein crystal sample acquisition loop base |
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| Publication number | Publication date |
|---|---|
| CN106596607B (en) | 2019-02-12 |
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