CN106596541A - Method for detecting alkali-doped cow milk - Google Patents
Method for detecting alkali-doped cow milk Download PDFInfo
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- CN106596541A CN106596541A CN201611181789.0A CN201611181789A CN106596541A CN 106596541 A CN106596541 A CN 106596541A CN 201611181789 A CN201611181789 A CN 201611181789A CN 106596541 A CN106596541 A CN 106596541A
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- milk
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- rhodizonic
- alkali
- ethanol solution
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a method for detecting alkali-doped cow milk. The method is characterized by comprising the following steps: preparing an ethanol solution with the mass percentage of 68 to 78 percent; preparing a rhodizonic acid liquid: adding sodium rhodizmate into the ethanol liquid prepared in the step A, wherein the concentration of the sodium rhodizmate is 0.4 to 0.6 g/L; and sucking 5 ml of a cow milk sample, injecting the sample into a test tube, adding 5 drops of the rhodizonic acid solution prepared in the step B, uniformly shaking, and observing the color of the milk.
Description
Technical field
The present invention relates to a kind of technical field of biological, more particularly to a kind of method that alkali Lac Bovis seu Bubali is mixed in detection.
Background technology
Milk is the necessary nutriment in many family daily lifes, containing rich in protein, fat and ammonia in milk
The nutritional labelings such as base acid, are the liquid food for easily being digested and being absorbed by human body.Milk is fragrant and sweet good to eat, nutritious, easy with its
The features such as digesting and assimilating and suiting the taste of both old and young, huge numbers of families are come into rapidly.But saying for most consumers may only know cattle
Milk is a kind of good nutriment, and but do not necessarily know the health hazard for having drunk adulterated milk to people, not to mention how to differentiate
Adulterated milk.Therefore, opportunity is caused to some illegal retailers.
The market potential of China's Dairy Consumption is huge, and milk and milk productses industrial expansion prospect is also very optimistic
's.At present the adulterated raw milk quality that improves of strike raw material milk is a problem for meriting attention.It is a kind of common that alkali is mixed in breast
Adulterated means.Plus alkali is, in order to shelter becoming sour for Lac Bovis seu Bubali, to reduce the acidity of Lac Bovis seu Bubali, prevents Lac Bovis seu Bubali from condensing because of souring.Cause
It is sodium carbonate (Na that this some illegal milk family adds the conventional alkali of a small amount of alkali in the Lac Bovis seu Bubali for becoming sour2CO3), sodium bicarbonate
(NaHCO3), sodium hydroxide (NaOH) etc., breast inferior is changed into into lattice breast.But not only flavour is not good to add the Lac Bovis seu Bubali after alkali, Er Qieyi
Corrupt bacteria growing is made, while some vitamin are also destroyed, it is unfavorable to drinking person health, therefore add the detection of alkali to have necessarily to sweet milk
Meaning.
The present invention is combined in ethanol experimental applications rose-red method detection raw milk and mixes alkali, by testing with reference to ethanol, really
The optimal concentration of ethanol is made, rosy optimum addition will set up a kind of simple, quick, practical qualitative detection milk
In mix the method for alkali.
The content of the invention
Based on the technical problem that background technology is present, the present invention proposes a kind of simple, quick, practical qualitative detection cattle
The method that alkali is mixed in milk.
Technical scheme is as follows：
It is a kind of to detect the method for mixing alkali Lac Bovis seu Bubali, comprise the following steps：
A, the ethanol solution that preparation mass fraction is 68-78%；
B, preparation rhodizonic acid solution：Rhodizonic acid, wherein rose-red are added in the ethanol solution prepared in step
The concentration of sour sodium is 0.4-0.6g/L；
In C, absorption 5ml milk sample injecting tubes, the rhodizonic acid ethanol solution 5 prepared in step B is added to drip,
Shake up, observe the color of milk.
Alternatively, the ethanol solution mass fraction described in step A be 70%, rhodizonic acid described in step B it is dense
Spend for 0.4g/L.
Alternatively, the ethanol solution mass fraction described in step A be 70%, rhodizonic acid described in step B it is dense
Spend for 0.6g/L.
Preferably, the ethanol solution mass fraction described in step A be 70%, rhodizonic acid described in step B it is dense
Spend for 0.5g/L.
The invention has benefit that：
The method is simple and practical, and simultaneously alkali is mixed in detection to meet ethanol experiment in raw material, improves work efficiency, is raw material
The alkali of mixing of breast is checked, there is provided a kind of new method.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein is not used to only to explain the present invention
Limit the present invention.
The description of specific distinct unless the context otherwise, although the step in the present invention is arranged with label, and
The precedence for limiting step is not used in, the order of step or the execution of certain step unless expressly stated needs other steps
Based on, the relative rank of otherwise step is adjustable.It is appreciated that term "and/or" used herein is related to
And and cover one of associated Listed Items or one or more of any and all possible combination.
Embodiment one
It is a kind of to detect the method for mixing alkali Lac Bovis seu Bubali, comprise the following steps：
A, respectively prepare mass fraction be 68%, 70%, 72%, 75%, 78% ethanol it is standby；
The preparation of B, rhodizonic acid solution：Weigh 0.04g (being accurate to 0.0001g) rhodizonic acid and be dissolved in 100ml respectively
The step of A prepare ethanol solution in be made into rhodizonic acid solution；
In C, absorption 5ml test sample injecting tubes, it is separately added into the rhodizonic acid ethanol solution 5 prepared in step B and drips,
Shake up, observe the color of milk, while doing blank experiment.
0.04g rhodizonic acids are dissolved in testing result in different ethanol concentration：
Detection in 68%, 70%, 72%, 75%, 78% concentration of alcohol is dissolved in respectively with 0.04g rhodizonic acids to mix not
With the milk of concentration NaOH, while doing blank experiment.As a result it is as shown in table 1 below:
The 0.04g rhodizonic acids of table 1 are dissolved in testing result in different ethanol concentration
It is dissolved in 68%, 70%, 72%, 75%, 78% concentration of alcohol respectively with 0.04g rhodizonic acids as shown in Table 1
Middle to detect that the milk minimum detectability for mixing variable concentrations NaOH color change occurs when being 0.08g/100ml, ethanol now is dense
Spend for 68%, 70% and 75%, but 70% ethanol is dissolved in judging 0.04g rhodizonic acids according to the sensitivity of color change
Color change is most obvious during concentration, therefore as shown in Table 1.Under the conditions of 0.04g rhodizonic acids, 70% concentration of alcohol detection is dissolved in
Milk mixes alkali best results.
Embodiment two
Embodiment two is in stepb, to weigh 0.05g (being accurate to 0.0001g) rose with the difference of embodiment one
Rhodizonic acid solution is made in the ethanol solution that the step of rare red acid sodium is dissolved in respectively 100ml A is prepared.
0.05g rhodizonic acids are dissolved in testing result in different ethanol concentration：
Detection in 68%, 70%, 72%, 75%, 78% concentration of alcohol is dissolved in respectively with 0.05g rhodizonic acids to mix not
With the milk of concentration NaOH, while doing blank experiment.As a result it is as shown in table 2 below:
The 0.05g rhodizonic acids of table 2 are dissolved in testing result in different ethanol concentration
It is dissolved in 68%, 70%, 72%, 75%, 78% concentration of alcohol respectively with 0.05g rhodizonic acids as shown in Table 2
Middle to detect that the milk minimum detectability for mixing variable concentrations NaOH color change occurs when being 0.06g/100ml, ethanol now is dense
Spend for 70% and 75%, but 70% concentration of alcohol is dissolved in judging 0.05g rhodizonic acids according to the sensitivity of color change
When color change it is most obvious, therefore as shown in Table 2, under the conditions of 0.05g rhodizonic acids, be dissolved in 70% concentration of alcohol detection milk
Mix alkali best results.
Embodiment three
Embodiment three is in stepb, to weigh 0.06g (being accurate to 0.0001g) rose with the difference of embodiment one
Rhodizonic acid solution is made in the ethanol solution that the step of rare red acid sodium is dissolved in respectively 100ml A is prepared.
0.06g rhodizonic acids are dissolved in testing result in different ethanol concentration
Detection in 68%, 70%, 72%, 75%, 78% concentration of alcohol is dissolved in respectively with 0.06g rhodizonic acids to mix not
With the milk of concentration NaOH, while doing blank experiment.As a result it is as shown in table 3 below:
The 0.06g rhodizonic acids of table 3 are dissolved in testing result in different ethanol concentration
It is dissolved in 68%, 70%, 72%, 75%, 78% concentration of alcohol respectively with 0.06g rhodizonic acids as shown in Table 3
Middle to detect that the milk minimum detectability for mixing variable concentrations NaOH color change occurs when being 0.08g/100ml, ethanol now is dense
Spend for 70% and 75%, but 70% concentration of alcohol is dissolved in judging 0.06g rhodizonic acids according to the sensitivity of color change
When color change it is most obvious, therefore as shown in Table 3, under the conditions of 0.06g rhodizonic acids, be dissolved in 70% concentration of alcohol detection milk
Mix alkali best results.
In sum, draw：The concentration of alcohol detection milk for being dissolved in 70% with 0.05g rhodizonic acids mixes alkali sensitivity most
It is good, it is most highly preferred embodiment of the invention.
The present invention technique effect be：The method is simple and practical, and simultaneously alkali is mixed in detection to meet ethanol experiment in raw material, carries
High work efficiency, is that raw milk mixes alkali inspection, there is provided a kind of new method.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any those familiar with the art the invention discloses technical scope in, technology according to the present invention scheme and its
Inventive concept equivalent or change in addition, all should be included within the scope of the present invention.
Claims (4)
1. it is a kind of to detect the method for mixing alkali Lac Bovis seu Bubali, it is characterised in that to comprise the following steps：
A, the ethanol solution that preparation mass fraction is 68-78%；
B, preparation rhodizonic acid solution：Rhodizonic acid, wherein rhodizonic acid are added in the ethanol solution prepared in step
Concentration be 0.4-0.6g/L；
In C, absorption 5ml milk sample injecting tubes, add the rhodizonic acid ethanol solution 5 prepared in step B to drip, shake
It is even, observe the color of milk.
2. the method for mixing alkali Lac Bovis seu Bubali is detected as claimed in claim 1, it is characterised in that the ethanol solution matter described in step A
Amount fraction is 70%, and the concentration of rhodizonic acid described in step B is 0.5g/L.
3. the method for mixing alkali Lac Bovis seu Bubali is detected as claimed in claim 1, it is characterised in that the ethanol solution matter described in step A
Amount fraction is 70%, and the concentration of rhodizonic acid described in step B is 0.4g/L.
4. the method for mixing alkali Lac Bovis seu Bubali is detected as claimed in claim 1, it is characterised in that the ethanol solution matter described in step A
Amount fraction is 70%, and the concentration of rhodizonic acid described in step B is 0.6g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611181789.0A CN106596541A (en) | 2016-12-20 | 2016-12-20 | Method for detecting alkali-doped cow milk |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611181789.0A CN106596541A (en) | 2016-12-20 | 2016-12-20 | Method for detecting alkali-doped cow milk |
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| CN201611181789.0A Pending CN106596541A (en) | 2016-12-20 | 2016-12-20 | Method for detecting alkali-doped cow milk |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065141A (en) * | 1992-01-26 | 1992-10-07 | 肇州县乳制品厂 | The preparation of fresh-milk testing agent and purposes |
| CN101029875A (en) * | 2007-04-10 | 2007-09-05 | 澳诺（中国）制药有限公司 | Card assembly for fastly inspecting lead content and its production |
| US20100029004A1 (en) * | 2008-08-01 | 2010-02-04 | Ribi Hans O | Duration and environmental monitoring compositions, devices methods for preparation and systems |
-
2016
- 2016-12-20 CN CN201611181789.0A patent/CN106596541A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065141A (en) * | 1992-01-26 | 1992-10-07 | 肇州县乳制品厂 | The preparation of fresh-milk testing agent and purposes |
| CN101029875A (en) * | 2007-04-10 | 2007-09-05 | 澳诺（中国）制药有限公司 | Card assembly for fastly inspecting lead content and its production |
| US20100029004A1 (en) * | 2008-08-01 | 2010-02-04 | Ribi Hans O | Duration and environmental monitoring compositions, devices methods for preparation and systems |
Non-Patent Citations (4)
| Title |
|---|
| 万萍: "《食品分析与实验》", 30 September 2015, 中国纺织出版社 * |
| 张彦明 等: "《动物性食品安全生产与检验技术》", 28 February 2009, 中国农业出版社 * |
| 朱丹丹: "《乳品加工技术》", 31 July 2013, 中国农业大学出版社 * |
| 李中林 等: "《有机试剂合成与应用》", 31 January 1988, 湖南省科学技术出版社 * |
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