CN106596498A - Device for quickly detecting air microorganisms - Google Patents
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- CN106596498A CN106596498A CN201710035782.6A CN201710035782A CN106596498A CN 106596498 A CN106596498 A CN 106596498A CN 201710035782 A CN201710035782 A CN 201710035782A CN 106596498 A CN106596498 A CN 106596498A
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- 244000005700 microbiome Species 0.000 title claims abstract description 14
- 238000001514 detection method Methods 0.000 claims abstract description 27
- 238000012545 processing Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 5
- 239000000523 sample Substances 0.000 claims description 24
- 230000003287 optical effect Effects 0.000 claims description 12
- 239000007850 fluorescent dye Substances 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 230000010365 information processing Effects 0.000 claims description 3
- 239000013618 particulate matter Substances 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 abstract description 6
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 230000003416 augmentation Effects 0.000 abstract 1
- 230000003321 amplification Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000001917 fluorescence detection Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000638935 Senecio crassissimus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000010276 collecting duct carcinoma Diseases 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
- G01N15/0211—Investigating a scatter or diffraction pattern
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/075—Investigating concentration of particle suspensions by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
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- General Health & Medical Sciences (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a device for quickly detecting air microorganisms, and belongs to the technical field of environmental monitoring. A laser light source, a convergent lens, a pinhole plate, a collimating lens, a diaphragm, a Fourier lens, a sample cell and a center detector are located on the same central axis and are sequentially arranged from left to right. The center detector is located on a focal plane of the right side of the Fourier lens and formed by the arrangement of photoelectric detection planes in a hemi-toroidal shape. The circular ring widths of the hemi-toroidal-shaped photoelectric detection planes are gradually increased from the center of a circle to outside and the center is hollow. A right-angle detector is located in the direction perpendicular to the central axis. An annular detector is located on an arc formed by the center detector and the right-angle detector and formed by a series of rectangular photoelectric detection planes which are archwise arranged at equal intervals. The right-angle detector, the annular detector, the center detector and a fluorescent detector are connected with a data processing unit through cables. According to the device, complicated operation processes, such as augmentation, culture and marking are avoided, and the device has real-time and efficient advantages.
Description
Technical field
The invention belongs to environmental monitoring technology field, and in particular to a kind of air microbe device for fast detecting.
Background technology
Air microbe detection technique environmental monitoring, the monitoring of pharmaceutical industry environmental standard etc. in anti-terrorism safety, the building
Field has wide application.But traditional air microbe detection technique, such as solid impact formula air microbe detection method
(Lu Yanying, Wang Jin, Zhu Rong, He Xingrong. indoor public places air microbe detects and method for disinfection and sterilization that 2008 years are national
The science meeting of wind and atmospheric environment, on October 21st, 2008, Beijing, pp.272-276), the detection of natural subsidence formula air microbe
Method (Chen E, Wan Dong, Chu Kecheng, Xu Shuqing, Zhang Ning. the monitoring of air microbial contamination and progress, development fields of environmental monitoring in china,
Volume 2014,30, the 4th phase, pp.171-178) and membrance separation detection method (Xu Dongming, tension and relaxation, Zhang Xiquan, Zhao Hongxia. micro-filtration membrane
Application of the isolation technics in terms of CDCs microorganism detection, Chinese countryside health, the 2015, the 12nd phase, pp.41-42) etc.,
The complex operations process such as sample collecting, culture, amplification, labelling is needed generally, required time is long, it is difficult to realize quick inspection in real time
Survey.Therefore, research and develop quick air microbe detection means to be of great significance and value.
The content of the invention
The sampling for being directed to the presence of conventional air microbial detection device of the invention, culture, amplification, labelling etc. are wasted time and energy,
The problem of quick real-time detection is difficult to, a kind of quick air microbe detection means is proposed.
Technical scheme：
A kind of air microbe device for fast detecting, including LASER Light Source, collecting lenses, pinhole plate, collimating lens, light
Door screen, fourier transform lenses, sample cell, right angle detector, annular detector, central detector, fluorescent probe, signal processing unit
And air pump；At LASER Light Source, collecting lenses, pinhole plate, collimating lens, diaphragm, fourier transform lenses, sample cell and central detector
On same central axis, from left to right arrange successively；Central detector is located on the focal plane on the right side of fourier transform lenses, center
Detector is rearranged by semi-circular shape photodetection face, the annular width in semi-circular shape photodetection face by justify center outwards by
Cumulative to add, center is hollow out；Right angle detector is located on central axis upright direction；Annular detector be located at central detector with
On the circular arc that right angle detector is constituted, annular detector is made up of a series of rectangle photodetection face of arc shootings at equal intervals；
Right angle detector, annular detector, central detector, fluorescent probe are connected with data processing unit by cable；
Detected that step is as follows with above-mentioned air microbe device for fast detecting：
A. the laser of LASER Light Source output is converged on focal plane through collecting lenses, and focal plane places pinhole plate, Jing
Pinhole plate incides collimating lens after filtering, and is expanded by collimating lens and incides diaphragm for directional light, the light of Jing diaphragms output by
Fourier transform lenses focusing illumination is on sample cell；
B. air pump is by air sample suction sample cell to be measured, sky of the laser Jing fourier transform lenses focusing illuminations in sample cell
Gas sample product；Particulate matter in air causes scattering of light, and scattered light is by right angle detector, annular detector and central detector
Detection；Light irradiation transmitting fluorescence is excited in microorganism in air, and fluorescence is detected by fluorescent probe；
C. right angle detector, annular detector and central detector detect scattering optical information, the fluorescent probe spy for obtaining
The fluorescence information for measuring distinguishes Jing cable transmission to signal processing unit；Signal processing unit is carried out according to optical scattering principle
Scattering optical information processing and analysis, obtain the size and number information of particulate in air；Signal processing unit is shone according to laser
The wavelength of fluorescence for penetrating microorganism generation analyzes the type and quantity for obtaining microorganism with strength information；The micro- life of air is realized finally
The species of thing, the quick detection of size and number information.
Beneficial effects of the present invention：
(1) present invention greatly reduces detection time without the need for operations such as amplification, culture and labellings, realizes to air sample
Quick detection.
(2) present invention is detected the size and number for obtaining particulate in air by signal processing unit using optical scattering,
The type and quantity of microorganism are obtained using fluorescence detection, it is to avoid the artificial observation of traditional detection method, with real-time high-efficiency
Advantage.
Description of the drawings
Fig. 1 is air microbe device for fast detecting figure of the present invention.
Fig. 2 is air microbe device for fast detecting top view of the present invention.
Fig. 3 is central detector structure chart of the present invention.
In figure：1 LASER Light Source；2 collecting lenses；3 pinhole plates；4 collimating lens；5 diaphragms；
6 fourier transform lenses；7 sample cells；81 right angle detectors；82 annular detectors；83 central detectors；
9 fluorescent probes；10 data processing units；11 air pumps.
Specific embodiment
Below in conjunction with accompanying drawing and technical scheme, the specific embodiment of the present invention is further illustrated.
Air microbe device for fast detecting of the present invention using optical scattering detect obtain particulate in air size and
Quantity, obtains the type and quantity of microorganism using fluorescence detection, realizes the quick detection of microbes in air.
Embodiment
Fig. 1 is air microbe device for fast detecting schematic diagram of the present invention.The device include LASER Light Source, collecting lenses,
Pinhole plate, collimating lens, diaphragm, fourier transform lenses, sample cell, right angle detector, annular detector, central detector, fluorescence
Detector, signal processing unit, air pump.
Fig. 2 is air microbe device for fast detecting top view of the present invention, described LASER Light Source, collecting lenses, pin hole
Plate, collimating lens, diaphragm, fourier transform lenses, sample cell, central detector are on same central axis.
The central detector is located on the focal plane on the right side of the fourier transform lenses of central axis.As shown in figure 3, center is visited
Survey device to be rearranged by semi-circular shape photodetection face, the annular width in semi-circular shape photodetection face is outside gradually by center is justified
Increase, circle center is hollow out.
The right angle detector is located on central axis upright direction.
The annular detector is located on the circular arc that central detector is constituted with right angle detector, and annular detector by one is
Arrange the rectangle photodetection face composition of arc shooting at equal intervals.
The scattering photo detecting unit, fluorescent probe are connected with data processing unit by cable.
Air microbe device for fast detecting follows the steps below detection：
The laser of LASER Light Source output is converged on focal plane through collecting lenses, and focal plane places pinhole plate, Jing pins
Orifice plate incides collimating lens after filtering, and is expanded by collimating lens and incides diaphragm for directional light, and the light of Jing diaphragms output is by Fu
Vertical leaf lens focuss are irradiated on sample cell.
Air pump is by air sample suction sample cell, air sample of the laser Jing fourier transform lenses focusing illuminations in sample cell
Product.Particulate matter in air causes scattering of light, and scattered light is by the right angle detector, annular detection scattered in photo detecting unit
Device and central detector detection.Light irradiation transmitting fluorescence is excited in microorganism in air, and fluorescence is detected by fluorescent probe.
Right angle detector, annular detector and central detector detect scattering optical information, the fluorescent probe detection for obtaining
The fluorescence information for obtaining distinguishes Jing cable transmission to signal processing unit.Signal processing unit is dissipated according to optical scattering principle
Optical information processing and analysis are penetrated, the size and number information of particulate in air is obtained.Signal processing unit is irradiated according to laser
The wavelength of fluorescence that microorganism is produced analyzes the type and quantity for obtaining microorganism with strength information.Air microbe is realized finally
Species, the quick detection of size and number information.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto.
Any those familiar with the art the present invention illustrate technical scope in, technology according to the present invention scheme and its
Inventive concept is replaced on an equal basis or is changed, and should all cover the row in protection scope of the present invention.
Claims (2)
1. a kind of air microbe device for fast detecting, it is characterised in that the air microbe device for fast detecting includes laser
It is light source (1), collecting lenses (2), pinhole plate (3), collimating lens (4), diaphragm (5), fourier transform lenses (6), sample cell (7), straight
Angle detector (81), annular detector (82), central detector (83), fluorescent probe (9), signal processing unit (10) are gentle
Pump (11)；LASER Light Source (1), collecting lenses (2), pinhole plate (3), collimating lens (4), diaphragm (5), fourier transform lenses (6), sample
Product pond (7) and central detector (83) are on same central axis, are from left to right arranged successively；Central detector (83) is located at
On focal plane on the right side of fourier transform lenses (6), central detector (83) is rearranged by semi-circular shape photodetection face, semicircular ring
The annular width in shape photodetection face is outwards gradually increased by center is justified, and center is hollow out；Right angle detector (81) is positioned at center
In axis vertical direction；Annular detector (82) on the circular arc that central detector (83) and right angle detector (81) are constituted,
Annular detector (82) is made up of a series of rectangle photodetection face of arc shootings at equal intervals；Right angle detector (81), annular
Detector (82), central detector (83), fluorescent probe (9) are connected with data processing unit (10) by cable.
2. the method for being detected with the air microbe device for fast detecting described in claim 1, it is characterised in that step is such as
Under：
A. the laser that LASER Light Source (1) is exported is converged on focal plane through collecting lenses (2), and focal plane places pinhole plate
(3) collimating lens (4) are incided Jing after pinhole plate (3) optical filtering, are expanded by collimating lens (4) and diaphragm are incided for directional light
(5) light that, Jing diaphragms (5) are exported is by fourier transform lenses (6) focusing illumination to sample cell (7)；
B., by air sample suction sample cell (7) to be measured, laser Jing fourier transform lenses (6) focusing illumination is to sample cell for air pump (11)
(7) air sample in；Particulate matter in air causes scattering of light, and scattered light is by right angle detector (81), annular detection
Device (82) and central detector (83) detection；Light irradiation transmitting fluorescence is excited in microorganism in air, and fluorescence is by fluorescent probe
(9) detect；
C. right angle detector (81), annular detector (82) and central detector (83) detect scattering optical information, the fluorescence for obtaining
The fluorescence information that detector (9) detection is obtained distinguishes Jing cable transmission to signal processing unit (10)；Signal processing unit (10)
Optical information processing and analysis are scattered according to optical scattering principle, the size and number information of particulate in air is obtained；Letter
Number processing unit (10) irradiates the wavelength of fluorescence that microorganism produces according to laser analyzes the kind for obtaining microorganism with strength information
Class and quantity, finally realize species, the quick detection of size and number information of air microbe.
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| CN201710035782.6A CN106596498B (en) | 2017-01-19 | 2017-01-19 | A kind of air microbe device for fast detecting |
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| CN201710035782.6A CN106596498B (en) | 2017-01-19 | 2017-01-19 | A kind of air microbe device for fast detecting |
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| CN106596498B CN106596498B (en) | 2018-09-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108645770A (en) * | 2018-07-24 | 2018-10-12 | 安徽尼古拉电子科技有限公司 | A kind of integrated laser haze detecting system based on computer vision |
| CN108844866A (en) * | 2018-07-06 | 2018-11-20 | 北京世纪朝阳科技发展有限公司 | Nano particle follow-up mechanism |
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| CN106596498B (en) | 2018-09-04 |
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