CN106596488A - Method for quick determination of sulfonamide residues in food - Google Patents
Method for quick determination of sulfonamide residues in food Download PDFInfo
- Publication number
- CN106596488A CN106596488A CN201611151342.9A CN201611151342A CN106596488A CN 106596488 A CN106596488 A CN 106596488A CN 201611151342 A CN201611151342 A CN 201611151342A CN 106596488 A CN106596488 A CN 106596488A
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- Prior art keywords
- sulfanilamide
- food
- enrichment phase
- phase
- sulfonamide
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
Abstract
The invention discloses a method for quick determination of sulfonamide residues in food. The method includes the technological steps of a, weighing the weight of the food with the sulfonamide residues accurately, adding the weighed food with the sulfonamide residues into distilled water, mixing the food evenly prior to centrifugation, and taking out liquid supernatant; b, preparing a sulfonamide fluorescent derivative to obtain a standard substance enrichment phase; c, preparing a sample sulfonamide fluorescent derivative to obtain a sample enrichment phase; d, spotting the standard substance enrichment phase obtained in the step b and the sample enrichment phase obtained in the step c on a silica gel plate, observing fluorescence intensity of the standard substance enrichment phase and the sample enrichment phase under an ultraviolet lamp with ultraviolet light wavelength of 365 nm, and judging the sulfonamide residues in the food through comparison between the fluorescence intensity of the standard substance enrichment phase and that of the sample enrichment phase. The method is capable of detecting the sulfonamide residues in the food rapidly and effectively according to the above technological step design.
Description
Technical field
The present invention relates to residual amount of sulfanilamide quick detection side in food detection method technical field, more particularly to a kind of food
Method.
Background technology
The animal-derived foods such as milk, interior product are generally abundant containing protein, fat, amino acid, carbohydrate, calcium, phosphorus, iron etc.
Nutriment, as people's living standard is constantly improved, milk, the meat products proportion in the diet structure of resident
It is increasing.
Wherein, sulfa drugs because its have the advantages that inexpensively, curative effect is high, short treating period, and is widely used in herding
In industry production, for preventing and treating livestock and poultry pestilence.The a large amount of use of sulfa drugs causes it in most animals source food
In have a residual, after the exceeded animal-derived food of the edible residual quantity of sulfonamide of people, health can be worked the mischief.
So, residual amount of sulfanilamide in food is detected and is just seemed and is highly desirable to.
The content of the invention
It is an object of the invention to provide residual amount of sulfanilamide method for quick in a kind of food, sulfanilamide (SN) residual in the food
Amount method for quick can be detected fast and effeciently to residual amount of sulfanilamide in food.
To reach above-mentioned purpose, the present invention is achieved through the following technical solutions.
Residual amount of sulfanilamide method for quick in a kind of food, includes following processing step, specially：
A, the weight for accurately weighing sulfanilamide (SN) residual food product, and the sulfanilamide (SN) residual food product for weighing is added into distilled water, distill
The consumption of water is 3-6ml/g, and mixing is centrifuged and takes out supernatant, standby；
B, prepare sulfanilamide (SN) fluorescent derivative：Configuration sulfanilamide (SN) concentration is the standard aqueous solution of 0.001-50mg/l, and toward above-mentioned standard
Add quality percent by volume for the fluorescamine methanol solution of 0.05-0.1% in the aqueous solution, reaction 30-40 minutes stood under normal temperature,
Then remove upper strata aqueous phase by the way that split-phase mode is centrifuged, to obtain standard items enrichment phase；
C, prepare sample sulfanilamide (SN) fluorescent derivative：The supernatant that step a is obtained is taken, and matter is added toward above-mentioned standard aqueous solution
Amount percent by volume is the fluorescamine methanol solution of 0.05-0.1%, reaction 30-40 minutes is stood under normal temperature, then by centrifugation point
Phase mode removes upper strata aqueous phase, to obtain example enrichment phase；
The example enrichment phase point sample that d, the standard items enrichment phase that step b is obtained and step c are obtained on silica gel plate, and
Violet wavelength is observation caliber product enrichment phase, the fluorescence intensity of example enrichment phase under the ultraviolet light of 365nm, by comparing standard items
The fluorescence intensity of the fluorescence intensity of enrichment phase and example enrichment phase to judge food in residual amount of sulfanilamide.
Wherein, in step b, the PH for adding the standard aqueous solution of fluorescamine methanol solution is 5-6.
Wherein, in step c, the PH for adding the supernatant of fluorescamine methanol solution is 5-6.
Beneficial effects of the present invention are：Residual amount of sulfanilamide method for quick in a kind of food of the present invention, its bag
Following processing step is included：A, the weight for accurately weighing sulfanilamide (SN) residual food product, and by the sulfanilamide (SN) residual food product for weighing add to
In distilled water, the consumption of distilled water is 3-6ml/g, and mixing is centrifuged and takes out supernatant, standby；B, prepare sulfanilamide (SN) fluorescent derivatization
Thing：Configuration sulfanilamide (SN) concentration is the standard aqueous solution of 0.001-50mg/l, and quality volume hundred is added toward above-mentioned standard aqueous solution
Divide the fluorescamine methanol solution than being 0.05-0.1%, reaction 30-40 minutes are stood under normal temperature, then gone by centrifugation split-phase mode
Fall upper strata aqueous phase, to obtain standard items enrichment phase；C, prepare sample sulfanilamide (SN) fluorescent derivative：The supernatant that step a is obtained is taken,
And add quality percent by volume for the fluorescamine methanol solution of 0.05-0.1% toward above-mentioned standard aqueous solution, stand under normal temperature anti-
30-40 minutes are answered, then removes upper strata aqueous phase by the way that split-phase mode is centrifuged, to obtain example enrichment phase；D, step b is obtained
Standard items enrichment phase and step c obtain example enrichment phase point sample on silica gel plate, and violet wavelength for 365nm purple
Observation caliber product enrichment phase, the fluorescence intensity of example enrichment phase under light lamp, by compare standard items enrichment phase fluorescence intensity with
The fluorescence intensity of example enrichment phase to judge food in residual amount of sulfanilamide.Designed by above-mentioned processing step, the present invention can
Fast and effeciently residual amount of sulfanilamide in food is detected.
Specific embodiment
With reference to specific embodiment, the present invention will be described.
Residual amount of sulfanilamide method for quick in a kind of food, includes following processing step, specially：
A, the weight for accurately weighing sulfanilamide (SN) residual food product, and the sulfanilamide (SN) residual food product for weighing is added into distilled water, distill
The consumption of water is 3-6ml/g, and mixing is centrifuged and takes out supernatant, standby；
B, prepare sulfanilamide (SN) fluorescent derivative：Configuration sulfanilamide (SN) concentration is the standard aqueous solution of 0.001-50mg/l, and toward above-mentioned standard
Add quality percent by volume for the fluorescamine methanol solution of 0.05-0.1% in the aqueous solution, reaction 30-40 minutes stood under normal temperature,
Then remove upper strata aqueous phase by the way that split-phase mode is centrifuged, to obtain standard items enrichment phase；
C, prepare sample sulfanilamide (SN) fluorescent derivative：The supernatant that step a is obtained is taken, and matter is added toward above-mentioned standard aqueous solution
Amount percent by volume is the fluorescamine methanol solution of 0.05-0.1%, reaction 30-40 minutes is stood under normal temperature, then by centrifugation point
Phase mode removes upper strata aqueous phase, to obtain example enrichment phase；
The example enrichment phase point sample that d, the standard items enrichment phase that step b is obtained and step c are obtained on silica gel plate, and
Violet wavelength is observation caliber product enrichment phase, the fluorescence intensity of example enrichment phase under the ultraviolet light of 365nm, by comparing standard items
The fluorescence intensity of the fluorescence intensity of enrichment phase and example enrichment phase to judge food in residual amount of sulfanilamide.
Wherein, in step b, the PH for adding the standard aqueous solution of fluorescamine methanol solution is 5-6；Likewise, the step
In rapid c, the PH for adding the supernatant of fluorescamine methanol solution is 5-6.
Designed by above-mentioned processing step, the present invention can be detected fast and effeciently to residual amount of sulfanilamide in food.
Above content is only presently preferred embodiments of the present invention, for one of ordinary skill in the art, according to the present invention's
Thought, will change in specific embodiments and applications, and this specification content should not be construed as to the present invention
Restriction.
Claims (3)
1. residual amount of sulfanilamide method for quick in a kind of food, it is characterised in that include following processing step, specially：
A, the weight for accurately weighing sulfanilamide (SN) residual food product, and the sulfanilamide (SN) residual food product for weighing is added into distilled water, distill
The consumption of water is 3-6ml/g, and mixing is centrifuged and takes out supernatant, standby；
B, prepare sulfanilamide (SN) fluorescent derivative：Configuration sulfanilamide (SN) concentration is the standard aqueous solution of 0.001-50mg/l, and toward above-mentioned standard
Add quality percent by volume for the fluorescamine methanol solution of 0.05-0.1% in the aqueous solution, reaction 30-40 minutes stood under normal temperature,
Then remove upper strata aqueous phase by the way that split-phase mode is centrifuged, to obtain standard items enrichment phase；
C, prepare sample sulfanilamide (SN) fluorescent derivative：The supernatant that step a is obtained is taken, and matter is added toward above-mentioned standard aqueous solution
Amount percent by volume is the fluorescamine methanol solution of 0.05-0.1%, reaction 30-40 minutes is stood under normal temperature, then by centrifugation point
Phase mode removes upper strata aqueous phase, to obtain example enrichment phase；
The example enrichment phase point sample that d, the standard items enrichment phase that step b is obtained and step c are obtained on silica gel plate, and
Violet wavelength is observation caliber product enrichment phase, the fluorescence intensity of example enrichment phase under the ultraviolet light of 365nm, by comparing standard items
The fluorescence intensity of the fluorescence intensity of enrichment phase and example enrichment phase to judge food in residual amount of sulfanilamide.
2. residual amount of sulfanilamide method for quick in a kind of food according to claim 1, it is characterised in that：The step
In b, the PH for adding the standard aqueous solution of fluorescamine methanol solution is 5-6.
3. residual amount of sulfanilamide method for quick in a kind of food according to claim 2, it is characterised in that：The step
In c, the PH for adding the supernatant of fluorescamine methanol solution is 5-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611151342.9A CN106596488A (en) | 2016-12-14 | 2016-12-14 | Method for quick determination of sulfonamide residues in food |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611151342.9A CN106596488A (en) | 2016-12-14 | 2016-12-14 | Method for quick determination of sulfonamide residues in food |
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| CN106596488A true CN106596488A (en) | 2017-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1858232A (en) * | 2006-03-27 | 2006-11-08 | 华中农业大学 | Method for screening sulfanilamide medicine residue in edible animal tissue and reagent kit |
| US20090029349A1 (en) * | 2002-09-27 | 2009-01-29 | Siemens Medical Solutions Diagnostics | Novel Applications of Acridinium Compounds and Derivatives in Homogeneous Assays |
| CN103760141A (en) * | 2014-01-14 | 2014-04-30 | 昆明理工大学 | Method for rapid detection of residual amount of sulfanilamide in food |
| CN105510428A (en) * | 2016-01-27 | 2016-04-20 | 武汉矽感科技有限公司 | Method for testing sulfamethoxazole in water by means of ion mobility spectrometer |
| WO2016151051A1 (en) * | 2015-03-23 | 2016-09-29 | Algipharma As | Use of alginate oligomers and cftr modulators in the treatment of conditions associated with cftr dysfunction |
-
2016
- 2016-12-14 CN CN201611151342.9A patent/CN106596488A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090029349A1 (en) * | 2002-09-27 | 2009-01-29 | Siemens Medical Solutions Diagnostics | Novel Applications of Acridinium Compounds and Derivatives in Homogeneous Assays |
| CN1858232A (en) * | 2006-03-27 | 2006-11-08 | 华中农业大学 | Method for screening sulfanilamide medicine residue in edible animal tissue and reagent kit |
| CN103760141A (en) * | 2014-01-14 | 2014-04-30 | 昆明理工大学 | Method for rapid detection of residual amount of sulfanilamide in food |
| WO2016151051A1 (en) * | 2015-03-23 | 2016-09-29 | Algipharma As | Use of alginate oligomers and cftr modulators in the treatment of conditions associated with cftr dysfunction |
| CN105510428A (en) * | 2016-01-27 | 2016-04-20 | 武汉矽感科技有限公司 | Method for testing sulfamethoxazole in water by means of ion mobility spectrometer |
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