CN106596376A - Test method of aperture distribution of hemodialysis membrane - Google Patents
Test method of aperture distribution of hemodialysis membrane Download PDFInfo
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- 238000001631 haemodialysis Methods 0.000 title claims abstract description 58
- 230000000322 hemodialysis Effects 0.000 title claims abstract description 58
- 239000012528 membrane Substances 0.000 title claims abstract description 55
- 238000010998 test method Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 34
- 238000005315 distribution function Methods 0.000 claims abstract description 18
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 9
- 239000012498 ultrapure water Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 14
- 239000011148 porous material Substances 0.000 claims description 12
- 239000002202 Polyethylene glycol Substances 0.000 claims description 7
- 230000001186 cumulative effect Effects 0.000 claims description 7
- 229920001223 polyethylene glycol Polymers 0.000 claims description 7
- 238000005227 gel permeation chromatography Methods 0.000 claims description 5
- 229920001917 Ficoll Polymers 0.000 claims description 4
- 229920001503 Glucan Polymers 0.000 claims description 4
- 230000009194 climbing Effects 0.000 claims description 4
- 238000009792 diffusion process Methods 0.000 claims description 3
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 claims description 3
- 238000012956 testing procedure Methods 0.000 claims description 3
- 238000000149 argon plasma sintering Methods 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 238000005374 membrane filtration Methods 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 33
- 238000001914 filtration Methods 0.000 abstract description 5
- 238000004088 simulation Methods 0.000 abstract 3
- 239000000706 filtrate Substances 0.000 abstract 1
- 229920002492 poly(sulfone) Polymers 0.000 description 10
- 238000011549 displacement method Methods 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 4
- 239000004744 fabric Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 238000001493 electron microscopy Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 208000009304 Acute Kidney Injury Diseases 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 101100004286 Caenorhabditis elegans best-5 gene Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 208000033626 Renal failure acute Diseases 0.000 description 1
- 231100000643 Substance intoxication Toxicity 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 206010070863 Toxicity to various agents Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 201000011040 acute kidney failure Diseases 0.000 description 1
- 208000012998 acute renal failure Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000020832 chronic kidney disease Diseases 0.000 description 1
- 208000022831 chronic renal failure syndrome Diseases 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007485 conventional hemodialysis Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 235000012489 doughnuts Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229920006335 epoxy glue Polymers 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920001195 polyisoprene Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
- G01N15/088—Investigating volume, surface area, size or distribution of pores; Porosimetry
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- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a test method of the aperture distribution of a hemodialysis membrane. The method comprises the following steps: dissolving one or more solutes in ultrapure water to form a simulation solution, and filtering the simulation solution through the hemodialysis membrane; testing the concentration of corresponding solute molecular weight in the simulation solution and the concentration of corresponding solute molecular weight in a filtrate; calculating a curve of the molecular diameter of the corresponding solute molecular weight and the interception rate of the hemodialysis membrane; establishing the aperture distribution function and the accumulated distribution function of the hemodialysis membrane; and carrying out nonlinear fitting on the accumulated distribution function on practically measured interception rate curve data through least squares fitting analysis software, determining the aperture distribution parameter, and substituting the aperture distribution parameter into the aperture distribution function to obtain the aperture distribution of the hemodialysis membrane. The method is used to rapidly detect the aperture distribution of the hemodialysis membrane., and has the advantages of high accuracy of measured data, simplicity and high practicality.
Description
Technical field
The present invention relates to hemodialysis membrane characterization technique field, and in particular to a kind of test of hemodialysis membrane pore-size distribution
Method.
Background technology
Hemodialyzer is commonly called as artificial kidney, is a kind of conventional hemodialysis utensil of blood purification, is mainly used in treatment
The symptoms such as chronic renal failure, acute renal failure and drug intoxication.Hemodialyzer mainly utilizes the principles such as disperse, convection current, absorption,
Small molecular material poisonous in blood is discharged, the beneficial macromole blood constituent such as erythrocyte, albumin is retained, so as to
Reach the purpose of purification blood.
In dialysis procedure, dialyser selects separating property mainly to be affected by hemodialysis membrane aperture and pore-size distribution.
Membrane aperture size refers to the channel diameter at most narrow place in the hole path for running through film surfaces externally and internally, and hemodialysis membrane aperture size
For nanometer scale.The membrane aperture assay method for adopting at present is numerous, such as electron microscopy, gas-liquid displacement method, liquid-liquid displacement method.Electricity
Mirror method is more directly perceived, but can only obtain local message, and have ignored blind hole and the impact of tortuosity;Needed for gas-liquid displacement method
Test pressure is larger, and required pressure is far above the use pressure of film, test result weight when testing for the dialyzer of small-bore
Existing property is not high, and accuracy is low;Liquid-liquid displacement method needs two kinds of immiscible liquid, and different system liquid interfacial tensions are with temperature
Change is obvious, and after liquid and hemodialysis membrane infiltration, often produces swelling, and the pore-size distribution fluctuation ratio measured is larger.
United States Patent (USP) (US2002/0060187 A1) discloses a kind of side of the pore-size distribution of test organic membrane or inoranic membrane
Method and device.The method is only capable of obtaining pore-size distribution of the tested film for different average molecular quantity of material, it is impossible to obtain tested
The pore-size distribution parameter of film, test result cannot accurately, intuitively characterize the pore-size distribution in effective hole of tested film.
The content of the invention
The present invention provides a kind of method of testing of hemodialysis membrane pore-size distribution, it is intended to solve above-mentioned pore-size distribution test side
The deficiency of method.The hemodialysis membrane pore-size distribution method of testing provided using the present invention, the pore-size distribution of acquisition has result accurate
Really, the features such as favorable reproducibility, high precision.
The present invention is as follows to solve the technical scheme that above-mentioned technical problem is adopted:
A kind of hemodialysis membrane pore-size distribution method, comprises the steps:
Step 1. is dissolved in ultra-pure water as simulated solution using one or several solutes, is filtered by hemodialysis membrane
Filtered solution is obtained afterwards;
Step 2. is divided using the molecular weight of the simulated solution and filtered solution obtained in molecular weight distribution method of testing testing procedure 1
Cloth, obtains respectively the concentration of correspondence molecular weight solute;
Step 3. calculates the molecular diameter d of correspondence molecular weight solute according to relational expression 1;
Relational expression 1
K is Boltzmann constant in relational expression 1, and T is the absolute temperature of solution, and η is the viscosity of solution, and D is solute molecule
Diffusion coefficient in the solution;
Step 4. obtains the rejection curve of correspondence molecular weight solute according to relational expression 2;
Relational expression 2
In relational expression 2 R be rejection, CpAnd CfThe respectively concentration of filtered solution and simulated solution;
Step 5. sets up the pore size distribution function of hemodialysis membrane and its corresponding cumulative distribution function, sees the He of relational expression 3
4;
Relational expression 3
D in relational expression 3pFor hemodialysis film hole diameter, μ and σ is pore-size distribution parameter, respectively meansigma methodss and standard
Difference;
Relational expression 4
Wherein
D in relational expression 4pFor hemodialysis film hole diameter, μ and σ is pore-size distribution parameter, respectively meansigma methodss and standard
Difference;
Step 6. is entered by least square fitting analysis software by rejection curve data is surveyed to cumulative distribution function
Row nonlinear fitting, determines pore-size distribution parameter μ and σ;
Step 7. brings pore-size distribution parameter μ and σ in hemodialysis membrane pore size distribution function formula into, that is, obtain its aperture point
Cloth.
Heretofore described solute includes that glucosan, ficoll, Polyethylene Glycol, polyethylene glycol oxide etc. have polydispersity
Polymer substance.
Heretofore described molecular weight solute distribution is 100~10000000Da.
Heretofore described simulated solution concentration range is 0.01~10g/L.
Heretofore described ultrapure water conductivity is less than 0.1 μ S/cm, and temperature is maintained at 20~25 DEG C.
Heretofore described simulated solution needs matching while using, the finished product that configuration is completed to use in best 5 hours at normal temperatures,
Otherwise larger is affected on test result.
The operating pressure scope filtered by hemodialysis membrane in heretofore described second step is 4~65kPa;It is preferred that
For 4~40kPa.
The heretofore described filtration stable experiment time is 10~30 minutes.
Heretofore described molecular weight distribution method of testing is gel permeation chromatography, light scattering method etc..
The analysis software of heretofore described carried out method of least square be Origin, Matlab, Lingo, 1stopt,
SPSS etc., can carry out nonlinear fitting.
Climbed the mountain from Lay Weinberg-wheat quart method, quasi- Newton method, simple face body in heretofore described nonlinear fitting
The optimized algorithms such as method, Differential evolution;It is preferred that Lay Weinberg-wheat quart method and simple face body climbing method.
Compared with prior art, technical advantage of the invention is:
1st, the method for testing of the hemodialysis membrane pore-size distribution of present invention design, based on spy of the membrane aperture in normal distribution
Point, for measured data analysiss, builds brand-new model to obtain pore-size distribution, compares gas-liquid displacement method, the displacement of liquid liquid
The prior arts such as method, can determine the pore-size distribution of the dialyzer in nanometer scale aperture, and little with data fluctuations, high precision
Advantage;
2nd, method of testing of the invention determines basis when the pore-size distribution performance of dialyzer is tested based on solute filtration,
Electron microscopy is compared, impact of the blind hole to test result can be avoided;
3rd, the present invention solves the characterization problems of hemodialysis membrane filtering accuracy, realizes the effective mistake to hemodialysis membrane
The measurement of the pore-size distribution of filter through hole, the research and use for hemodialysis membrane provides a kind of practical and convenient performance evaluation side
Method.
Description of the drawings
Fig. 1 is the polysulfones high pass hemodialysis membrane pore-size distribution measurement result schematic diagram of the embodiment of the present invention 1;
Fig. 2 is the polysulfones low pass hemodialysis membrane pore-size distribution measurement result schematic diagram of the embodiment of the present invention 2;
Fig. 3 is the polysulfones hemodiafiltration membrane aperture measure of spread result schematic diagram of the embodiment of the present invention 3.
Specific embodiment
Below in conjunction with specific embodiment, the technical scheme in the embodiment of the present invention is further elaborated.It is aobvious
So, described embodiment is only, to explain the present invention, to be not intended to limit the present invention.
The present invention devises a kind of hemodialysis membrane pore size distribution determination method, comprises the steps:
Step 1. is dissolved in ultra-pure water as simulated solution using one or several solutes, is filtered by hemodialysis membrane
Filtered solution is obtained afterwards;
Step 2. is divided using the molecular weight of the simulated solution and filtered solution obtained in molecular weight distribution method of testing testing procedure 1
Cloth, obtains respectively the concentration of correspondence molecular weight solute;
Step 3. calculates the molecular diameter d (nm) of correspondence molecular weight solute according to relational expression 1;
Relational expression 1
K (erg/K) is Boltzmann constant in relational expression 1, and T (K) is the absolute temperature of solution, and η (Pas) is solution
Viscosity, D (cm2/ s) it is solute molecule diffusion coefficient in the solution;
Step 4. obtains the rejection curve of correspondence molecular weight solute according to relational expression 2;
Relational expression 2
In relational expression 2 R be rejection, CpAnd C (g/L)f(g/L) it is respectively the concentration of filtered solution and simulated solution;
Step 5. sets up the pore size distribution function of hemodialysis membrane and its corresponding cumulative distribution function, sees the He of relational expression 3
4;
Relational expression 3
D in relational expression 3p(nm) it is hemodialysis film hole diameter, μ and σ is pore-size distribution parameter, and μ is hemodialysis fenestra
Footpath distribution average, σ are hemodialysis membrane pore-size distribution hole standard deviation;
Relational expression 4
Wherein
D in relational expression 4p(nm) it is hemodialysis film hole diameter, μ and σ is pore-size distribution parameter, and μ is hemodialysis fenestra
Footpath distribution average, σ are hemodialysis membrane pore-size distribution hole standard deviation;
Step 6. is entered by least square fitting analysis software by rejection curve data is surveyed to cumulative distribution function
Row nonlinear fitting, determines pore-size distribution parameter μ and σ;
Step 7. brings pore-size distribution parameter μ and σ in hemodialysis membrane pore size distribution function formula into, that is, obtain its aperture point
Cloth.
Heretofore described relational expression 3 is the pore size distribution function of hemodialysis membrane, and relational expression 4 is hemodialysis membrane
The cumulative distribution function in aperture.
Embodiment 1
Take certain brand polysulfones high pass hemodialyzer on market.Using various glucosans as test solute, its is heavy respectively
Son amount is respectively 1kDa, 10kDa, 50kDa and 100kDa, is dissolved in ultra-pure water as simulated solution.Simulated solution Jing polysulfones high passes
Hemodialyzer is filtered, and obtains filtered solution after experiment operation after stable 30min, and operating pressure is 10~20kPa.By simulated solution and
Filtered solution carries out molecular weight distribution test using gel permeation chromatography, obtains the corresponding glucosan of each molecular weight in two kinds of solution
Concentration.Using relational expression 1 and 2, cutoff performance curve of this dialyzer to each molecular weight dextran is obtained.By cutoff performance data
Nonlinear fitting is carried out to relational expression 4 by SPSS softwares, fitting adopts Lay Weinberg-wheat quart method, determine that pore-size distribution is joined
Number μ and σ, in bringing relational expression 3 into, you can obtain its pore size distribution curve.Implement 5 parallel tests and obtain pore-size distribution parameter μ
And σ, as shown in table 1 is affected on result repeatability by pore-size distribution parameter relative standard deviation analysis test method.Fig. 1 is
The high pass hemodialysis membrane pore size distribution curve that 1 parallel test is measured, its pore-size distribution it is interval for 1.2~15.4nm it
Between.
The parallel test test result of 1 embodiment of table 1 and repeatability
Embodiment 2
Take certain brand polysulfones low pass hemodialyzer on market.Using various Polyethylene Glycol as test solute, its is heavy
Molecular weight is 1kDa, 6kDa, 12kDa and 35kDa, is dissolved in ultra-pure water as simulated solution.Simulated solution Jing polysulfones low pass is dialysed
Device is filtered, and obtains filtered solution after experiment operation after stable 30min, and operating pressure is 10~20kPa.By simulated solution and filtered solution profit
Molecular weight distribution test is carried out with gel permeation chromatography, the corresponding Polyethylene glycol of each molecular weight in two kinds of solution is obtained.
Using relational expression 1 and 2, cutoff performance curve of this dialyzer to each molecular weight polyethylene glycol is obtained.Cutoff performance data are led to
Crossing 1stopt softwares carries out nonlinear fitting to relational expression 4, fitting using simple face body climbing method, determine pore-size distribution parameter μ and
σ, in bringing relational expression 3 into, you can obtain its pore size distribution curve, as shown in Fig. 2 the low pass hemodialysis membrane pore-size distribution is interval
Between 0.2~9nm.
Embodiment 3
Take certain brand polysulfones filter for blood dialysis on market.Using various ficolls as test solute, its is heavy respectively
Son amount is respectively 1kDa, 20kDa, 50kDa and 100kDa, is dissolved in ultra-pure water as simulated solution.Simulated solution Jing polysulfones blood
Dialysis filter is filtered, and obtains filtered solution after experiment operation after stable 30min, and operating pressure is 10~20kPa.By simulated solution and
Filtered solution carries out molecular weight distribution test using gel permeation chromatography, obtains the corresponding ficoll of each molecular weight in two kinds of solution
Concentration.Using relational expression 1 and 2, cutoff performance curve of this dialyzer to each molecular weight polyisoprene sucrose is obtained.Cutoff performance data are led to
Crossing 1stopt softwares carries out nonlinear fitting to relational expression 4, fitting using simple face body climbing method, determine pore-size distribution parameter μ and
σ, in bringing relational expression 3 into, you can obtain its pore size distribution curve, as shown in figure 3, the hemodiafiltration membrane aperture distributed area
Between 1.4~11.2nm.
The test number of aperture distributed constant in the embodiment 2 and 3 of table 2
Embodiment is numbered | μ | σ |
2 | 1.03 | 1.96 |
3 | 2.84 | 1.54 |
Comparative example 1
From with the same polysulfones high pass hemodialyzer of embodiment 1, its pore-size distribution is tested using liquid-liquid displacement method.It is real
Middle employing isopropanol and pure water are tested as test fluid and wetting liquid, test pressure is 0~250kPa, respectively obtains hemodialysis
The main line of film and wet line.Its pore-size distribution is obtained according to dry and wet line, it is 5~21nm that the high pass hemodialysis membrane pore-size distribution is interval
Between.
Can draw from embodiment 1 and comparative example 1:Liquid-liquid displacement method needs with epoxy glue seal film to be measured when testing
Silk, experimentation needs respectively pressurization successively to obtain main line and wet line, and the method for testing of the present invention directly tests hemodialyzer,
Test can be completed under a transmembrane pressure, method of testing is simple to operation.
Comparative example 2
From with the same high pass hemodialyzer of embodiment 1, its hole is tested using field emission scanning electron microscope method
Footpath is distributed.One section of film silk is intercepted in experiment, is fixed on the sample stage of ultramicroscope, metal spraying after being dried shoots doughnut
The electron microscopic picture of film inner surface.The analysis of electron microscopic picture Jing graphics softwares obtains its pore-size distribution, and distribution is 10~95nm.
Can draw from embodiment 1 and comparative example 2, electron microscopy test pore-size distribution only tests the aperture of inner surface,
The impact of tortuosity and blind hole is have ignored, test result is bigger than normal.Based on filtration experiment, test is effectively to filter logical to the present invention
The pore-size distribution in hole, test result high precision.
The above instantiation is only explanation of the invention, is not limitation of the present invention;Simultaneously for this
The those skilled in the art in field, under the premise without departing from the principles of the invention, can also make some improvements and modifications, and these change
Enter and retouch also to should be regarded as protection scope of the present invention.
Claims (8)
1. a kind of method of testing of hemodialysis membrane pore-size distribution, comprises the steps:
Step 1. is dissolved in ultra-pure water as simulated solution using one or several solutes, after being filtered by hemodialysis membrane
Obtain filtered solution;
Step 2. using the simulated solution obtained in molecular weight distribution method of testing testing procedure 1 and the molecular weight distribution of filtered solution,
The concentration of correspondence molecular weight solute is obtained respectively;
Step 3. calculates the molecular diameter d of correspondence molecular weight solute according to relational expression 1;
Relational expression 1
K is Boltzmann constant in relational expression 1, and T is the absolute temperature of solution, and η is the viscosity of solution, and D is solute molecule molten
Diffusion coefficient in liquid;
Step 4. obtains the rejection curve of correspondence molecular weight solute according to relational expression 2;
Relational expression 2
In relational expression 2 R be rejection, CpAnd CfThe respectively concentration of filtered solution and simulated solution;
Step 5. sets up the pore size distribution function of hemodialysis membrane and its corresponding cumulative distribution function, sees relational expression 3 and 4;
Relational expression 3
D in relational expression 3pFor hemodialysis film hole diameter, μ and σ is pore-size distribution parameter, respectively meansigma methodss and standard deviation;
Relational expression 4
Wherein
D in relational expression 4pFor hemodialysis film hole diameter, μ and σ is pore-size distribution parameter, respectively meansigma methodss and standard deviation;
Step 6. will survey rejection curve data cumulative distribution function is carried out by least square fitting analysis software it is non-
Linear fit, determines pore-size distribution parameter μ and σ;
Step 7. brings pore-size distribution parameter μ and σ in hemodialysis membrane pore size distribution function formula into, that is, obtain its pore-size distribution.
2. a kind of hemodialysis membrane pore-size distribution method of testing according to claim 1, it is characterised in that the solute is
The polymer substance with polydispersity such as glucosan, ficoll, Polyethylene Glycol, polyethylene glycol oxide.
3. hemodialysis membrane pore-size distribution method of testing according to claim 1, it is characterised in that the molecular weight solute
Distribution is 100~10000000Da.
4. hemodialysis membrane pore-size distribution method of testing according to claim 1, it is characterised in that the ultra-pure water conductance
Rate is less than 0.1 μ S/cm, and temperature is maintained at 20~25 DEG C.
5. hemodialysis membrane pore-size distribution method of testing according to claim 1, it is characterised in that lead to described in second step
The operating pressure scope for crossing hemodialysis membrane filtration is 4~65kPa.
6. hemodialysis membrane pore-size distribution method of testing according to claim 1, it is characterised in that the molecular weight distribution
Method of testing is gel permeation chromatography, light scattering method etc..
7. hemodialysis membrane pore-size distribution method of testing according to claim 1, is characterised by described carried out minimum
The analysis software of square law is Origin, Matlab, Lingo, 1stopt, SPSS etc., can carry out nonlinear fitting.
8. hemodialysis membrane pore-size distribution method of testing according to claim 1, is characterised by described nonlinear fitting
Any one in middle selection Lay Weinberg-wheat quart method, quasi- Newton method, simple face body climbing method, Differential evolution.
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日本膜学会: "《膜分离过程设计法》", 30 September 1988, 科学技术文献出版社 * |
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CN109224867B (en) * | 2018-09-21 | 2021-08-03 | 湖州师范学院求真学院 | Characterization method of microporous membrane aperture |
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