CN106596233A - Dyeing and decoloration method of two-dimensional electrophoresis gel Coomassie brilliant blue for screening saline-alkaline resistant plant material - Google Patents
Dyeing and decoloration method of two-dimensional electrophoresis gel Coomassie brilliant blue for screening saline-alkaline resistant plant material Download PDFInfo
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- CN106596233A CN106596233A CN201611217165.XA CN201611217165A CN106596233A CN 106596233 A CN106596233 A CN 106596233A CN 201611217165 A CN201611217165 A CN 201611217165A CN 106596233 A CN106596233 A CN 106596233A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 33
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 title claims abstract description 24
- 238000012216 screening Methods 0.000 title claims abstract description 14
- 239000000463 material Substances 0.000 title abstract 2
- 238000000539 two dimensional gel electrophoresis Methods 0.000 title 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 56
- 239000012153 distilled water Substances 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 41
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 239000003755 preservative agent Substances 0.000 claims abstract description 7
- 230000002335 preservative effect Effects 0.000 claims abstract description 7
- 238000011010 flushing procedure Methods 0.000 claims description 21
- 238000004720 dielectrophoresis Methods 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 13
- 239000003513 alkali Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 13
- 239000011780 sodium chloride Substances 0.000 claims description 13
- 239000005418 vegetable material Substances 0.000 claims description 13
- 238000010186 staining Methods 0.000 claims description 12
- 238000002845 discoloration Methods 0.000 claims description 9
- 238000012505 colouration Methods 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 3
- 241000218922 Magnoliophyta Species 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 239000012192 staining solution Substances 0.000 abstract 7
- 239000000243 solution Substances 0.000 abstract 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 31
- 108090000623 proteins and genes Proteins 0.000 description 8
- 239000000975 dye Substances 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 6
- 238000001704 evaporation Methods 0.000 description 4
- 230000008020 evaporation Effects 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 3
- 208000002109 Argyria Diseases 0.000 description 2
- 241000967218 Selaginella tamariscina Species 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000003351 Brassica cretica Nutrition 0.000 description 1
- 235000003343 Brassica rupestris Nutrition 0.000 description 1
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 235000010460 mustard Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a dyeing and decoloration method of two-dimensinal electrophoresis gel Coomassie brilliant blue for screening a saline-alkaline resistant plant material. The method comprises the first step of preparing a staining solution containing Coomassie brilliant blueR250, methyl alcohol, acetic acid and distilled water with a constant volume, uniformly blending the staining solution and still standing for 0.5-1.5 hours, filtering the staining solution and then storing the staining solution at a normal temperature for use; the second step of preparing a destaining solution containing ethyl alcohol, acetic acid and distilled water with a constant volume; the third step of using distilled water to wash two-dimensional SDS gel for 2-3 times, putting the two-dimensional SDS gel in a dyeing box, adding the staining solution into the dyeing box, using a preservative film to seal the dyeing box, and conducting dyeing on the two-dimensional SDS gel on a table concentrator for 2-3 hours at the normal temperature; the fourth step of conducting decoloration, wherein the decoloration comprises the procedures of pouring out the staining solution, using the distilled water to wash the gel for 2-4 times, adding the destaining solution, wherein the dosage of the destaining solution is 1-2 times that of the staining solution, conducting decoloration on the gel on the table concentrator for 2-3 hours at the normal temperature, and using the distilled water to wash the gel for 2-4 times. According to the dyeing and decoloration method, the method is further optimized on the basis of a routine technique method, procedures of pre-immobilization and the like are omitted, the speed of the dyeing and the decoloration is fast, and the effects of the dyeing and the decoloration are good.
Description
Technical field
The invention belongs to technical field of molecular biology, is related to a kind of using the two-way electricity of coomassie brilliant blue R250 rapid dyeing
Swimming gel and the method for decolouring.
Background technology
Because the function of gene is mainly realized by the protein of its coding, using proteomic techniques gene work(is annotated
It can be considered as the main part in post-genomic study.At present, classical proteomics research method is to prepare albumen
Sample, carries out dielectrophoresis, then obtains clearly protein profiling by dyeing, then by cutting compared with, digestion, Mass Spectrometric Identification etc.
Technology, obtains target protein.In the process, according to the property of destination protein, using the inspection that different sds gels is suitable for
Survey method in order to reduce loss of proteins as far as possible, improves the sensitivity of detection detecting two-way gel, and colouring method is always in quilt
Constantly explore, consider the conditioned basics such as sensitivity, the range of linearity, comfort level, cost and imaging device type enterprising
Row is selected.At present conventional dielectrophoresis colouring method is coomassie brilliant blue staining, silver staining and fluorescent staining.Silver staining it is sensitive
Degree is high, but easily produces very high background, causes albumen resolution loss, and waste time and energy, it is costly and need more to contact
Toxic chemical substance.Although fluorescent staining has high sensitivity, background is low match with mass spectrum, need special scanner with
Fluorescent dye, so there is a problem of that dyeing cost is higher.
The content of the invention
It is an object of the invention to provide a kind of dielectrophoresis gel Coomassie brilliant blue dye of screening Saline alkali tolerance vegetable material
Color and discoloration method.
The purpose of the present invention is achieved through the following technical solutions：
The dielectrophoresis gel coomassie brilliant blue staining and discoloration method of a kind of screening Saline alkali tolerance vegetable material, including such as
Lower step：
The preparation of step one, dyeing liquor：Prepare and contain 0.1～0.15wt% coomassie brilliant blue R250s, 40～50vol% first
Alcohol, 10～20vol% acetic acid, the dyeing liquor of distilled water constant volume, after mixing 0.5～1.5 hour is stood, and room temperature preservation is standby after filtration
With.According to gel size, dyeing liquor consumption is adjusted.
The preparation of step 2, destainer：Prepare fixed containing 20～30vol% ethanol, 10～15vol% acetic acid, distilled water
The destainer of appearance.
Step 3, dyeing：Two-way sds gel distilled water flushing 2～3 times, in being put into colouration box, adds dyeing liquor, dyeing
Liquid did not had gel；Sealed using preservative film, under room temperature condition, dyeed 2～3 hours on shaking table.
Step 4, decolouring：Pour out dyeing liquor, distilled water flushing gel 3～4 times；Destainer, destainer consumption is added to be dye
1～2 times of color liquid consumption, under room temperature condition, decolourizes 2～3 hours on shaking table, distilled water flushing 3～4 times.
The invention has the advantages that：
1st, the method scope on probation is wide, for most angiosperms such as salt mustard, arabidopsis, crudefiber crop and cryptogam
Such as Selaginella tamariscina all has preferable effect.
2nd, in the enterprising one-step optimization of convenient technical process, the program such as fixed, dyes and decolorization rate the present invention before dispensing
Hurry up, effect it is good.
3rd, the method operates more simple, low cost, gel clear background, with higher sensitivity.
Description of the drawings
Fig. 1 is Color figure.
Specific embodiment
Technical scheme is further described below in conjunction with the accompanying drawings, but is not limited thereto, it is every to this
Inventive technique scheme is modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention, all should be covered
In protection scope of the present invention.
Specific embodiment one：Present embodiments provide for a kind of dielectrophoresis gel of screening Saline alkali tolerance vegetable material
Coomassie brilliant blue staining and discoloration method, specific implementation step is as follows：
The preparation of step one, dyeing liquor：Prepare and contain 0.1～0.15wt% coomassie brilliant blue R250s, 40～50vol% first
Alcohol, 10～20vol% acetic acid, the dyeing liquor of distilled water constant volume, after mixing 0.5～1.5 hour is stood, and room temperature preservation is standby after filtration
With.According to gel size, dyeing liquor consumption is can adjust.
The preparation of step 2, destainer：Prepare fixed containing 20～30vol% ethanol, 10～15vol% acetic acid, distilled water
The destainer of appearance.
Step 3, dyeing：Two-way sds gel distilled water flushing 2～3 times, is gently put in colouration box, is slowly added into dyeing
Liquid, dyeing liquor did not had gel；Solution evaporation is prevented using preservative film sealing, under room temperature condition, slow shake dye on shaking table
Color 2～3 hours.
Step 4, decolouring：Pour out dyeing liquor, distilled water flushing gel 3～4 times；It is slowly added into destainer, destainer consumption
For 1～2 times of dyeing liquor consumption, under room temperature condition, slow shake on shaking table is decolourized 2～3 hours, distilled water flushing 3～4
It is secondary.
Specific embodiment two：From unlike specific embodiment one, step one is replaced with present embodiment：Preparation contains
There are 0.1～0.15wt% coomassie brilliant blue R250s, 30～40vol% ethanol, 10～15vol% acetic acid, the dye of distilled water constant volume
Color liquid, after mixing 0.5～1.5 hour is stood, and room temperature is saved backup after filtration.
Specific embodiment three：From unlike specific embodiment one, step 4 is replaced with present embodiment：Pour out dye
Color liquid, distilled water flushing gel 3～4 times is slowly added into destainer, and destainer consumption is 1～2 times of dyeing liquor consumption,
Under room temperature condition, slow shake on shaking table is decolourized 1～2 hour.After distilled water flushing 3～4 times, according to gel color throw, plus
Enter the destainer containing 5～8vol% ethanol, 8～10vol% acetic acid, carry out second decolouring, slow shake decolouring 1 on shaking table
～2 hours.
Specific embodiment four：Present embodiments provide for a kind of dielectrophoresis gel of screening Saline alkali tolerance vegetable material
Coomassie brilliant blue staining and discoloration method, specific implementation step is as follows：
The preparation of step one, dyeing liquor：Prepare containing 0.125wt% coomassie brilliant blue R250s, 45vol% methyl alcohol,
The dyeing liquor of 15vol% acetic acid, distilled water constant volume, after mixing 1 hour is stood, and room temperature is saved backup after filtration.
The preparation of step 2, destainer：Prepare containing 25vol% ethanol, 12.5vol% acetic acid, distilled water constant volume it is de-
Color liquid.
Step 3, dyeing：Two-way sds gel distilled water flushing 3 times, is gently put in colouration box, is slowly added into dyeing liquor,
Dyeing liquor did not had gel；Solution evaporation is prevented using preservative film sealing, under room temperature condition, slow shake on shaking table is dyeed about
3 hours.
Step 4, decolouring：Pour out dyeing liquor, distilled water flushing gel 3 times；Destainer is slowly added into, decolouring liquid measure is dye
1～2 times of color liquid consumption, slow shake is decolourized 2.5 hours on shaking table under room temperature, distilled water flushing 3 times.
When detecting Selaginella tamariscina plant using the method for present embodiment, Color is as shown in Figure 1.
Specific embodiment five：Present embodiments provide for a kind of dielectrophoresis gel of screening Saline alkali tolerance vegetable material
Coomassie brilliant blue staining and discoloration method, specific implementation step is as follows：
The preparation of step one, dyeing liquor：Prepare and contain 0.1wt% coomassie brilliant blue R250s, 40vol% methyl alcohol, 20vol%
The dyeing liquor of acetic acid, distilled water constant volume, after mixing 1.5 hours are stood, and room temperature is saved backup after filtration.
The preparation of step 2, destainer：Prepare containing 30vol% ethanol, 10vol% acetic acid, distilled water constant volume decolouring
Liquid.
Step 3, dyeing：Two-way sds gel distilled water flushing 2～3 times, is gently put in colouration box, is slowly added into dyeing
Liquid, dyeing liquor did not had gel；Solution evaporation is prevented using preservative film sealing, under room temperature condition, slow shake dye on shaking table
Color 3 hours.
Step 4, decolouring：Dyeing liquor is poured out, distilled water flushing gel 4 times is slowly added into destainer, and destainer consumption is
1～2 times of dyeing liquor consumption, under room temperature condition, slow shake on shaking table is decolourized 2 hours, after distilled water flushing 3～4 times,
According to gel protein burl dyeing and background color throw, the destainer containing 6vol% ethanol, 9vol% acetic acid is added, carried out
Decolourize for second, slow shake on shaking table is decolourized 1.5 hours.
Specific embodiment six：Present embodiments provide for a kind of dielectrophoresis gel of screening Saline alkali tolerance vegetable material
Coomassie brilliant blue staining and discoloration method, specific implementation step is as follows：
The preparation of step one, dyeing liquor：Prepare containing 0.15wt% coomassie brilliant blue R250s, 30vol% ethanol,
The dyeing liquor of 10vol% acetic acid, distilled water constant volume, after mixing 0.5 hour is stood, and room temperature is saved backup after filtration.
The preparation of step 2, destainer：Prepare containing 20vol% ethanol, 15vol% acetic acid, distilled water constant volume decolouring
Liquid.
Step 3, dyeing：Two-way sds gel distilled water flushing 3 times, is gently put in colouration box, is slowly added into dyeing liquor,
Dyeing liquor did not had gel；Solution evaporation is prevented using preservative film sealing, under room temperature condition, slow shake dyeing 2 on shaking table
Hour.
Step 4, decolouring：Pour out dyeing liquor, distilled water flushing gel 3～4 times；It is slowly added into destainer, destainer consumption
For 1～2 times of dyeing liquor consumption, under room temperature condition, slow shake on shaking table is decolourized 3 hours, distilled water flushing 3～4 times.
Claims (4)
1. the dielectrophoresis gel coomassie brilliant blue staining and discoloration method of a kind of screening Saline alkali tolerance vegetable material, its feature exists
It is as follows in the dielectrophoresis gel coomassie brilliant blue staining and discoloration method step of the screening Saline alkali tolerance vegetable material：
The preparation of step one, dyeing liquor：Prepare containing 0.1～0.15wt% coomassie brilliant blue R250s, 40～50vol% methyl alcohol,
The dyeing liquor of 10～20vol% acetic acid, distilled water constant volume, after mixing 0.5～1.5 hour is stood, and room temperature is saved backup after filtration；
The preparation of step 2, destainer：Prepare containing 20～30vol% ethanol, 10～15vol% acetic acid, distilled water constant volume
Destainer；
Step 3, dyeing：Two-way sds gel distilled water flushing 2～3 times, in being put into colouration box, adds dyeing liquor, dyeing liquor not to have
Cross gel；Sealed using preservative film, under room temperature condition, dyeed 2～3 hours on shaking table；
Step 4, decolouring：Pour out dyeing liquor, distilled water flushing gel 2～4 times；Destainer is added, destainer consumption is dyeing liquor
1～2 times of consumption, under room temperature condition, decolourizes 2～3 hours on shaking table, distilled water flushing 2～4 times.
2. the dielectrophoresis gel coomassie brilliant blue staining of screening Saline alkali tolerance vegetable material according to claim 1 and de-
Color method, it is characterised in that the step one is replaced with：Prepare containing 0.1～0.15wt% coomassie brilliant blue R250s, 30～
40vol% ethanol, 10～15vol% acetic acid, the dyeing liquor of distilled water constant volume, stand 0.5～1.5 hour, after filtration after mixing
Room temperature preservation is standby.
3. the dielectrophoresis gel coomassie brilliant blue staining of screening Saline alkali tolerance vegetable material according to claim 1 and de-
Color method, it is characterised in that the step 4 is replaced with：Dyeing liquor is poured out, distilled water flushing gel 2～4 times is slowly added into de-
Color liquid, destainer consumption is 1～2 times of dyeing liquor consumption, under room temperature condition, slowly shakes on shaking table and decolourizes 1～2 hour, steams
After distilled water rinses 2～4 times, according to gel color throw, the decolouring containing 5～8vol% ethanol, 8～10vol% acetic acid is added
Liquid, carries out second decolouring, and slow shake on shaking table is decolourized 1～2 hour.
4. the dielectrophoresis gel coomassie brilliant blue staining of screening Saline alkali tolerance vegetable material according to claim 1 and de-
Color method, it is characterised in that the Saline alkali tolerance vegetable material is angiosperm or cryptogam.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611217165.XA CN106596233A (en) | 2016-12-26 | 2016-12-26 | Dyeing and decoloration method of two-dimensional electrophoresis gel Coomassie brilliant blue for screening saline-alkaline resistant plant material |
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|---|---|---|---|
| CN201611217165.XA CN106596233A (en) | 2016-12-26 | 2016-12-26 | Dyeing and decoloration method of two-dimensional electrophoresis gel Coomassie brilliant blue for screening saline-alkaline resistant plant material |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109632434A (en) * | 2018-12-25 | 2019-04-16 | 苏州译酶生物科技有限公司 | The Coomassie brilliant blue rapid dye liquor and dyeing-decolorzing method of a kind of low stimulation of low toxicity |
| CN113049345A (en) * | 2019-12-26 | 2021-06-29 | 常州天地人和生物科技有限公司 | Electrophoresis gel dyeing and decoloring method |
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| CN103267791A (en) * | 2013-05-25 | 2013-08-28 | 杭州市农业科学研究院 | Method for acquiring two-dimensional strawberry electrophoresis differential protein map |
| CN103884765A (en) * | 2014-02-21 | 2014-06-25 | 杭州市农业科学研究院 | Method for obtaining two-dimensional electrophoresis difference protein map of chili pepper anther |
| CN104049026A (en) * | 2014-06-23 | 2014-09-17 | 天津商业大学 | Zymogram method for screening microbial extracellular collagenase |
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2016
- 2016-12-26 CN CN201611217165.XA patent/CN106596233A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103267791A (en) * | 2013-05-25 | 2013-08-28 | 杭州市农业科学研究院 | Method for acquiring two-dimensional strawberry electrophoresis differential protein map |
| CN103884765A (en) * | 2014-02-21 | 2014-06-25 | 杭州市农业科学研究院 | Method for obtaining two-dimensional electrophoresis difference protein map of chili pepper anther |
| CN104049026A (en) * | 2014-06-23 | 2014-09-17 | 天津商业大学 | Zymogram method for screening microbial extracellular collagenase |
| CN104049026B (en) * | 2014-06-23 | 2016-04-27 | 天津商业大学 | A kind of zymogram method for screening the outer clostridiopetidase A of extracellular microbial |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109632434A (en) * | 2018-12-25 | 2019-04-16 | 苏州译酶生物科技有限公司 | The Coomassie brilliant blue rapid dye liquor and dyeing-decolorzing method of a kind of low stimulation of low toxicity |
| CN109632434B (en) * | 2018-12-25 | 2021-03-26 | 苏州译酶生物科技有限公司 | Low-toxicity and low-irritation Coomassie brilliant blue rapid dyeing liquid and dyeing and decoloring method |
| CN113049345A (en) * | 2019-12-26 | 2021-06-29 | 常州天地人和生物科技有限公司 | Electrophoresis gel dyeing and decoloring method |
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