CN106596212A - Dopamine solid-phase extraction consumable material and preparation method thereof - Google Patents
Dopamine solid-phase extraction consumable material and preparation method thereof Download PDFInfo
- Publication number
- CN106596212A CN106596212A CN201610671683.2A CN201610671683A CN106596212A CN 106596212 A CN106596212 A CN 106596212A CN 201610671683 A CN201610671683 A CN 201610671683A CN 106596212 A CN106596212 A CN 106596212A
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- Prior art keywords
- extraction
- dopamine
- solid
- consumable material
- phase
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
Abstract
Solid-phase extraction (SPE) is a multipurpose sample pretreatment technology, can be used for the purification and concentration of samples, can improve the detection sensitivity of analytes, effectively eliminates interference components, and has broad application prospects. In recent years, a solid phase microextraction (SPME) method is also greatly developed, and the SPME method is less in extraction phase dosage and higher in analyte selectivity, and a solute is easier to elute. The invention discloses a preparation method of an extraction consumable material with dopamine as a bridge. In the method, the natural binder dopamine is used as a medium to form self polymerization in certain pH value environment, a poly dopamine layer is formed on the surface of an extraction material, and the extraction material is fixed on the extraction consumable material. The obtained equipment can keep the physical and chemical activity of the extraction material, and the extraction consumable material shows good extraction performances, and can be widely used in the development and production of ion exchange adsorption extraction, hydrophilic and oleophilic extraction and special functional affinity adsorption equipment. The method is simple, economical, non-toxic and environmentally-friendly.
Description
Technical field
Patent of the present invention is related to prepare Solid-Phase Extraction consumptive material.
Technical background
Solid-Phase Extraction is to analyze the focus of Sample Pretreatment Technique Used development, can be used for the separation of analyte in sample, pure
Change and be enriched with.Solid-Phase Extraction can be used for the process of gas, liquid and solid sample, be widely applied to medical treatment, food safety, ring
The fields such as border detection, commodity inspection, chemical industry Quality Control.In recent years, micro-extracting method has obtained significant progress, large-scale with traditional
Extraction is compared, and the micro-extraction response rate is higher, separates more effective, and speed is faster, extraction phase consumption is less, selectivity is higher, pre-
Processing procedure is simpler, more convenient operation.And with automatization and may with associated with the detection meanss such as chromatograph, electrophoresis.So
And, solidification of the solid phase material on extraction consumptive material is a problem all the time.Traditional method carrys out fixed extractor phase using chemical adhesive,
But gained extract will such as be applied to medicine food field and there is potential safety hazard.The method of another kind of fixed extractor material makes
Single or multiple lift filter membrane is used, preparation technology is cumbersome for the technology, porous membrane nature is different from fiber material, so as to artificial
Multilamellar of coordinating heterogeneity medium and the absorption to analyte and eluting produce interference.It is many that the present invention successfully uses biological adhesive
Bar amine solidifies fiber material, without filter membranous layer, reduces dead volume, and accurately control analysis elution time, reduces sample loss.
Dopamine is a kind of natural glue, in being widely present in the secretions of seashells animal.The chemistry of dopamine
Formula is C6H3 (OH) 2-CH2-CH2-NH2, is possess phenyl ring, the small-molecule substance of bis-phenol base and amino.Research it has been proved that
Under the conditions of suitable pH, dopamine can carry out auto polymerization in various material surfaces, form poly-dopamine layer.This is namely assorted
Shell-fish can remain to being attached on hard rock tightly under the repeat impact of stormy waves.Adhesion process is related to difference
The strong and weak interaction of level, including hydrogen bond action, chelation, interaction and covalent effect.Its research is become
Biological adhesive application and an important directions of development.
The content of the invention
The present invention carrys out fixed extractor phase using the auto polymerization effect of dopamine, prepares Solid-Phase Extraction and solid-phase microextraction consumption
Material.Dopamine is dissolved in solution during bonding, and solution used can be pure water, sulfate, hydrochlorate, nitrate, phosphate, boric acid
Salt, carbonate, trishydroxymethylaminomethane, ammonium salt and glycine, TRICINE, BICINE, TES, CHES, HEPES, MES,
The both sexes buffer solution such as MOPs.
Dopamine solution concentration is more than 1 mg/ml before bonding.PH value between 5-12, preferred range 6-9.5.
The extraction consumptive material of preparation includes liquid-transfering gun suction nozzle, porous sample process plate, syringe, disk like extracting disk, other can
The container and micron or Nano grade granule of energy shape.
In extraction consumptive material preparation process, first the little sky for only flowing for liquid is manufactured in extraction container with physical method
Between, physical method includes using filter membrane, forms the methods such as aperture, pressure initiation gap.After adding appropriate extraction solid phase, subsequently add
Enter dopamine solution, after stopping appropriate time, unnecessary dopamine solution pressure or gravity are discharged.
Said method can be used for forming short filter membranous layer and filter element layer, and more extractions of the same race subsequently can be superimposed thereon
Material, or other kinds of fiber material formation compound extracting equipment.
After fiber material solidification, original gap structure for only flowing for liquid can cut off in extraction container, it is also possible to
Retain.
Description of the drawings
Technical scheme in order to be illustrated more clearly that the embodiment of the present invention, below will be to making needed for embodiment description
Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present invention.Its
In：
1st, Fig. 1 is glucosan ladder of the example 1 obtained by extraction；
2nd, Fig. 2 is glucosan ladder of the example 2 obtained by extraction;
3rd, specific protein of the example 3 obtained by extraction in Fig. 3.
Specific embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention, rather than the embodiment of whole.Based on the embodiment in the present invention, this area is common
All other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
Example 1 prepares porous graphite suction nozzle and carries out oligosaccharide purification
1. porous graphite mixed solution forms aaerosol solution.Liquid-transfering gun suction nozzle exports to form aperture.Appropriate graphite suspension is added,
Remove liquid.The Dopamine Hydrochloride solution that addition is prepared in right amount.
2. static five minutes, solution was removed.Then wait for fiber material solidification in suction nozzle.
3. oligosaccharide uses respectively before purification appropriate NaOH solution, fiber material in acetum and the fine cleaning suction nozzle of second.
4. plus containing oligosaccharide sample, make sample be in close contact carbon-coating, wait five minutes and slowly release sample with vacuum or gravity again
Solution.
5. using fiber material in pure water and acetum cleaning suction nozzle.
6., using the precipitation liquid cleaning suction nozzle of 50 μ L, collecting outflow solution carries out chromatography.
Example 2 prepares ion exchange resin suction nozzle and carries out oligosaccharide purification
1. ion exchange resin forms aaerosol solution.Liquid-transfering gun suction nozzle exports to form aperture.Quantity of resin suspension is rapidly added,
Remove liquid.The Dopamine Hydrochloride solution that addition is prepared in right amount.
2. static a period of time, solution is removed.Then wait for fiber material solidification in suction nozzle.
3. more resin aaerosol solutions are added, expands adsorption layer volume.
4. oligosaccharide uses respectively before purification appropriate NaOH solution, resin in hydrochloric acid solution and pure water cleaning suction nozzle.
5. plus containing oligosaccharide sample, make sample be in close contact resin bed, wait five minutes and slowly released with vacuum or gravity again
Sample solution.
6. the warm and fine water mixed liquid of second cleans fiber material in suction nozzle.
7., using the precipitation liquid cleaning suction nozzle of 50 μ L, collecting outflow solution carries out chromatography.
Example 3 prepares protein extraction suction nozzle and carries out the purification of specific protein
1. resin particle forms aaerosol solution.Liquid-transfering gun suction nozzle exports to form aperture.Quantity of resin suspension is rapidly added, is removed
Liquid.The Dopamine Hydrochloride solution that addition is prepared in right amount.
2. static a period of time, solution is removed using air or oxygen.Then wait for 1-30 minutes.
3. the IgG solution for certain specific protein is added, some hours are waited.
4. add pure water to remove IgG solution, and continue cleaning three times.
5. add and be intended to purified protein samples, make sample be in close contact resin bed, it is slow with vacuum or gravity again to wait five minutes
Release sample solution.
6. using fiber material in PBS solution cleaning suction nozzle.
7., using the precipitation liquid cleaning suction nozzle of 50 μ L, collecting outflow solution carries out chromatography.
Embodiments of the invention are the foregoing is only, the scope of the claims of the present invention is not thereby limited, it is every using this
Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, is included within the scope of the present invention.
Claims (3)
1. one kind uses dopamine to prepare at Solid-Phase Extraction consumptive material, including liquid-transfering gun suction nozzle, porous sample as binding agent production
Reason plate, syringe, disk like extracting disk and micron or Nano grade granule.
2. take off the method that a kind of production of use dopamine prepares Solid-Phase Extraction consumptive material, its extraction step include the loading of consumptive material sample,
Chaff interference is washed out and object is washed.
3. as claimed in claim 1 or 2 to extract consumptive material, its filling fiber material includes porous material, granular materialss, porous
Property material include porous graphite, granular materialss include different-grain diameter resin and surface porosity granular materialss.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671683.2A CN106596212A (en) | 2016-08-16 | 2016-08-16 | Dopamine solid-phase extraction consumable material and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671683.2A CN106596212A (en) | 2016-08-16 | 2016-08-16 | Dopamine solid-phase extraction consumable material and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106596212A true CN106596212A (en) | 2017-04-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610671683.2A Pending CN106596212A (en) | 2016-08-16 | 2016-08-16 | Dopamine solid-phase extraction consumable material and preparation method thereof |
Country Status (1)
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014069853A1 (en) * | 2012-10-29 | 2014-05-08 | Kolon Industries, Inc. | Aramid fiber product with excellent conductivity and method of manufacturing the same |
| CN104098156A (en) * | 2014-07-30 | 2014-10-15 | 中国科学院新疆理化技术研究所 | Application of poly-dopamine composite magnetic micro-nano particles in oily sewage treatment |
-
2016
- 2016-08-16 CN CN201610671683.2A patent/CN106596212A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014069853A1 (en) * | 2012-10-29 | 2014-05-08 | Kolon Industries, Inc. | Aramid fiber product with excellent conductivity and method of manufacturing the same |
| CN104098156A (en) * | 2014-07-30 | 2014-10-15 | 中国科学院新疆理化技术研究所 | Application of poly-dopamine composite magnetic micro-nano particles in oily sewage treatment |
Non-Patent Citations (2)
| Title |
|---|
| JUAN ZHANG ET AL.: "Polydopamine-based immobilization of zeolitic imidazolate framework-8 for in-tube solid-phase microextraction", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| WENPENG ZHANG ET AL.: "Universal Multilayer Assemblies of Graphene in Chemically Resistant Microtubes for Microextraction", 《ANALYTICAL CHEMISTRY》 * |
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Application publication date: 20170426 |
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