CN106591404A - Wheat protein based extraction method - Google Patents
Wheat protein based extraction method Download PDFInfo
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- CN106591404A CN106591404A CN201510661946.7A CN201510661946A CN106591404A CN 106591404 A CN106591404 A CN 106591404A CN 201510661946 A CN201510661946 A CN 201510661946A CN 106591404 A CN106591404 A CN 106591404A
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Abstract
The invention provides a wheat protein based extraction method. A method for preparation of exorphin by hydrolysis of wheat protein isolate is carried out according to the steps of: 1. putting wheat protein isolate into alkaline protease to conduct hydrolysis at a temperature of 23-55DEG C and under a pH of 7.5-9.1 for 1-2h, with the dosage of alkaline protease being 50-70U in per gram of wheat protein isolate; and 2. putting the alkaline protease hydrolyzed wheat protein isolate into pepsin, trypsin, acidic protease or neutral protease to conduct hydrolysis for 4-5h, and controlling the dosage of pepsin being 3450-4456U in per gram of soybean protein isolate.
Description
Technical field
The invention belongs to food processing technology field, more particularly to a kind of extraction side based on wheat protein
Method.
Background technology
Cathepsin belongs to the papain superfamily of cysteine proteinase.Cysteine or sulfydryl
Protease be responsible for proteolytic activity site have well known to a cysteine residues, milk protein by
Casein and lactalbumin are constituted.Milk surum is remaining clear liquid after milk removing fat and casein, newborn
Albumin includes lactalbumin and lactoglobulin and other albumen.These protein components be with known method from
Separate in lactalbumin.Soybean protein can be removed in the residue produced after Oleum Glycines from Semen sojae atricolor and obtained
Arrive.This albumen has been characterized in that bean orangutan taste, so as to limit its purposes, and a histidine and one
Agedoite.This superfamily also has a glutamine in its oxygen anion hole.
The content of the invention
To solve above-mentioned technical problem, the technical solution used in the present invention is：It is a kind of based on wheat protein
Extracting method, hydrolyzed wheat separation albumen prepares the method for External irradiation and follows the steps below：First, it is little
Wheat separation albumen is put in alkaline protease and is hydrolyzed, and hydrolysis temperature is 23～55 DEG C, and pH is
7.5～9.1, the consumption of every gram of wheat separation protein neutral and alkali protease is 50～70U, and hydrolysis time is
1～2h；2nd, put into Jing after hydrolysis by novo pepsin, trypsin, acid protease or
Be hydrolyzed in neutral protease, hydrolysis time be 4～5h, pepsin in every gram of soybean protein isolate
Consumption be 3500～4500U；Acid protease hydrolysiss condition is：Hydrolysis temperature is 42～48 DEG C, pH
For 2.5～3.5, the consumption of acid protease is 5000～7000U in every gram of soybean protein isolate；Trypsin
Enzyme hydrolysis condition is：Hydrolysis temperature is 58～62 DEG C, and pH is 7.5～8.5, in every gram of soybean protein isolate
Tryptic consumption is 7000～9000U.
Further：Also include carrying out purification to destination protein：Destination protein is combined with diastatic starch
Domain albumen and the little modified protein of ubiquitin-like blend expression, obtain and include diastatic starch binding domain egg
The expression product of the little modified protein of in vain-ubiquitin-like-destination protein fusion protein.
Further：Yeast protein and the adding proportion of lactoprotein are 1: 1-5: 1 in composite protein beverage, are moved
Thing albumen is 1: 2 with the adding proportion of vegetable protein.
Further：Casein, milk protein concentrate (MPC), whey protein, whey protein concentration
Thing (WPC), whey protein sepd (WPI), alpha-lactalbumin, beta lactoglobulin and
Lactoferrin
Further：Weight percent shared by each face liber composition in the novel protein fiber blended fabric
Than being specially：The casein fiber accounts for 34%, and the wool keratin fiber accounts for 21%, the treasure
Globin fiber accounts for 16%, and the silkworm protein fiber accounts for 29%.
Further：The protein that canola protein separator is presented is distributed as：The 2S eggs of 60-95% weight
White matter
Further：Albumen a) or b) is 25: 1 to 1: 100 with the mol ratio of the Denatured protein
Further：The protein be milk or cheese whey protein, lactalbumin, beta lactoglobulin,
Serum albumin, immunoglobulin or soybean protein.
The present invention has the advantages and positive effects that：Can establish with the increasing of cellular level in skin aging
Age becomes the evaluation system for turning to index.Using this evaluation system, compared with previous methods, can in more detail,
The Degree of Ageing of skin is judged exactly.IgK chain V-III (KAU cold agglutinins), SP-40 precursor,
Ig AI Bur, the glycoprotein precursor rich in histidine, α -2-HS glycoproteins, the same work of gelsolin
Type a precursor, inhibitor Kunitz type protease.
Specific embodiment
The specific embodiment of the present invention is illustrated below：A kind of extracting method based on wheat protein,
Hydrolyzed wheat separation albumen prepares the method for External irradiation and follows the steps below：First, wheat separation protein
Put in alkaline protease and be hydrolyzed, hydrolysis temperature be 23～55 DEG C, pH be 7.5～9.1, per gram
The consumption of wheat separation protein neutral and alkali protease is 50～70U, and hydrolysis time is 1～2h；2nd, Jing alkali
Put in pepsin, trypsin, acid protease or neutral protease after property protease hydrolysiss
Water-filling solution, hydrolysis time is 4～5h, and pepsic consumption is in every gram of soybean protein isolate
3500～4500U；Acid protease hydrolysiss condition is：Hydrolysis temperature is 42～48 DEG C, and pH is 2.5～3.5,
The consumption of acid protease is 5000～7000U in every gram of soybean protein isolate；Trypsin hydrolyzing condition
For：Hydrolysis temperature is 58～62 DEG C, and pH is 7.5～8.5, tryptic in every gram of soybean protein isolate
Consumption is.Also include carrying out purification to destination protein：By destination protein and diastatic starch binding domain egg
The white and little modified protein of ubiquitin-like blends expression, obtain include diastatic starch binding domain albumen-
The expression product of the little modified protein of ubiquitin-like-destination protein fusion protein.Yeast egg in composite protein beverage
It is in vain 1: 1-3: 1 with the adding proportion of lactoprotein, animal proteinum is with the adding proportion of vegetable protein
1∶2.Casein, milk protein concentrate (MPC), whey protein, whey protein concentrate
(WPC), whey protein sepd (WPI), alpha-lactalbumin, beta lactoglobulin and
Percentage by weight tool shared by each face liber composition in novel protein fiber blended fabric described in newborn ferrum egg
Body is：The casein fiber accounts for 24%, and the wool keratin fiber accounts for 21%, the Margarita egg
White fiber accounts for 15%, and the silkworm protein fiber accounts for 29%.The protein that canola protein separator is presented
It is distributed as：Albumen described in the 2S protein of 60-25% weight a) or b) rubs with the Denatured protein
Your ratio is milk or cheese whey protein, lactalbumin, β for protein described in 25: 1 to 1: 100
Lactoglobulin, serum albumin, immunoglobulin or soybean protein.
Embodiments of the invention have been described in detail above, but the content is only the preferable of the present invention
Embodiment, it is impossible to be considered as the practical range for limiting the present invention.It is all according to the scope of the invention made it is equal
Deng change and improvement etc., all should still belong within this patent covering scope.
Claims (8)
1. a kind of extracting method based on wheat protein, it is characterised in that：Hydrolyzed wheat separation albumen prepares the method for External irradiation and follows the steps below：First, wheat separation protein is put in alkaline protease and is hydrolyzed, and hydrolysis temperature is 23～55 DEG C, and pH is 7.5～9.1, and the consumption of every gram of wheat separation protein neutral and alkali protease is 50～70U, and hydrolysis time is 1～2h；2nd, put into Jing after hydrolysis by novo in pepsin, trypsin, acid protease or neutral protease and be hydrolyzed, hydrolysis time is 4～5h, pepsic consumption is 3450～4456U in every gram of soybean protein isolate；Acid protease hydrolysiss condition is：Hydrolysis temperature is 42～44 DEG C, and pH is 2.5～3.5, and the consumption of acid protease is 3500～4600U in every gram of soybean protein isolate；Trypsin hydrolyzing condition is：Hydrolysis temperature is 56～46 DEG C, and pH is 7.5～8.5, and tryptic consumption is 7000～9000U in every gram of soybean protein isolate.
2. a kind of extracting method based on wheat protein according to claim 1, it is characterised in that：Also include carrying out purification to destination protein：Destination protein and diastatic starch binding domain albumen and the little modified protein of ubiquitin-like are blended into expression, the expression product comprising the little modified protein-destination protein fusion protein of diastatic starch binding domain albumen-ubiquitin-like is obtained.
3. a kind of extracting method based on wheat protein according to claim 1, it is characterised in that：Yeast protein and the adding proportion of lactoprotein are 1: 1-5: 1 in composite protein beverage, and animal proteinum is 1: 2 with the adding proportion of vegetable protein.
4. a kind of extracting method based on wheat protein according to claim 1, it is characterised in that：Casein, milk protein concentrate (MPC), whey protein, whey protein concentrate (WPC), whey protein sepd (WPI), alpha-lactalbumin, beta lactoglobulin and lactoferrin.
5. a kind of extracting method based on wheat protein according to claim 1, it is characterised in that：Percentage by weight shared by each face liber composition is specially in the novel protein fiber blended fabric：The casein fiber accounts for 34%, and the wool keratin fiber accounts for 21%, and the pearl protein fiber accounts for 17%, and the silkworm protein fiber accounts for 34%.
6. a kind of extracting method based on wheat protein according to claim 1, it is characterised in that：The protein that canola protein separator is presented is distributed as：The 2S protein of 34-55% weight.
7. a kind of extracting method based on wheat protein according to claim 1, it is characterised in that：Albumen a) or b) is 34: 1 to 1: 50 with the mol ratio of the Denatured protein.
8. a kind of extracting method based on wheat protein according to claim 1, it is characterised in that：The protein is milk or cheese whey protein, lactalbumin, beta lactoglobulin, serum albumin, immunoglobulin or soybean protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510661946.7A CN106591404A (en) | 2015-10-14 | 2015-10-14 | Wheat protein based extraction method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510661946.7A CN106591404A (en) | 2015-10-14 | 2015-10-14 | Wheat protein based extraction method |
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| CN201510661946.7A Pending CN106591404A (en) | 2015-10-14 | 2015-10-14 | Wheat protein based extraction method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230265139A1 (en) * | 2020-09-30 | 2023-08-24 | Nobell Foods, Inc. | Recombinant milk proteins and food compositions comprising the same |
| CN117099869A (en) * | 2023-06-27 | 2023-11-24 | 河南工业大学 | Preparation method of wheat protein peptide chelated calcium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907559A (en) * | 2012-11-15 | 2013-02-06 | 温志明 | Process for industrial production of hydrolyzed wheat protein |
| CN104054893A (en) * | 2014-06-30 | 2014-09-24 | 安徽瑞福祥食品有限公司 | Industrial preparation method of wheat protein hydrolysate |
-
2015
- 2015-10-14 CN CN201510661946.7A patent/CN106591404A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907559A (en) * | 2012-11-15 | 2013-02-06 | 温志明 | Process for industrial production of hydrolyzed wheat protein |
| CN104054893A (en) * | 2014-06-30 | 2014-09-24 | 安徽瑞福祥食品有限公司 | Industrial preparation method of wheat protein hydrolysate |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230265139A1 (en) * | 2020-09-30 | 2023-08-24 | Nobell Foods, Inc. | Recombinant milk proteins and food compositions comprising the same |
| US11952606B2 (en) * | 2020-09-30 | 2024-04-09 | Nobell Foods, Inc. | Food compositions comprising recombinant milk proteins |
| CN117099869A (en) * | 2023-06-27 | 2023-11-24 | 河南工业大学 | Preparation method of wheat protein peptide chelated calcium |
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