CN106591389B - A method of fumidil is produced using aspergillus fumigatus - Google Patents
A method of fumidil is produced using aspergillus fumigatus Download PDFInfo
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- CN106591389B CN106591389B CN201510667060.3A CN201510667060A CN106591389B CN 106591389 B CN106591389 B CN 106591389B CN 201510667060 A CN201510667060 A CN 201510667060A CN 106591389 B CN106591389 B CN 106591389B
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Abstract
The present invention relates to a kind of method using aspergillus fumigatus production fumidil (fumagillin), include the following steps: that the aspergillus fumigatus is inoculated in GMM solid medium carries out actication of culture, the GMM Medium's PH Value is 5-7；CYA solid medium, CXMA solid medium or LMM fluid nutrient medium are inoculated in after strain spore after activation is prepared spore suspension, the CYA solid medium, the CXMA solid medium pH value are natural, the LMM fluid nutrient medium pH value is adjusted to 5-7, cultivates under the conditions of 25 DEG C to 37 DEG C；The aspergillus fumigatus for collecting culture extracts fumidil and fumagillin derivatives.The present invention is screened for the carbon nitrogen source and microelement for influencing aspergillus fumigatus growth and fumidil, and is optimized to corresponding microelement concentration, and higher fumidil yield is obtained.
Description
Technical field
The present invention relates to field of microbial fermentation, and in particular to a kind of to produce fumidil using aspergillus fumigatus
(fumagillin) method.
Background technique
Aspergillus fumigatus (Aspergillus fumigatus) is a kind of important fungi, widely distributed in nature, is existed
In the fur that soil and animal are rotten.Aspergillus fumigatus is conditioned pathogen and important cause of disease, the conidium of generation in air,
Enter human body by the respiratory tract of people, the special states patient such as immunologic hypofunction or flora imbalance is caused to infect.On the other hand,
Such as gliotoxin (gliotoxin), fumidil (fumagillin) a variety of mycotoxins of aspergillus fumigatus generation are tight in feed
The health of grievous hurt animal simultaneously reduces production capacity, threatens the food safety and health of the mankind.In recent years, aspergillus fumigatus full genome
The completion of group sequencing provides strong tool for the mycotoxin research in aspergillus fumigatus source.The study found that fumidil can also
Using the biological control being used for as drug in the treatment and agricultural production of human diseases.
Fumidil can be used as a kind of angiogenesis inhibitors (angiogenesis), have compared with powerful antitumor angiogenesis
Effect.Angiogenesis is closely related with tumour growth, is of great significance for tumour growth, infiltration and transfer, prevents tumour
The formation of blood vessel may also inhibit the growth of tumour.People researched and developed can destroy and inhibit angiogenesis, effectively
Ground prevent growth and metastasis of tumours drug, wherein fumidil can specificity inhibit vascular endothelial cell proliferation, effect
Target is Methionine Aminopeptidase 2 (MetAP-2).The study found that fumidil can completely inhibit basic fibroblast life
The growth of vascular endothelial cell under long factor existence and mouse tumor angiogenesis, but have and cause weight loss etc. bright
Aobvious toxic side effect.Fumidil basic hydrolysis generates aspergillus fumigatus cedrol and derivative artificial synthesized on this basis can be used as one kind
Vasoinhibitor can play Antineoplastic angiogenesis, increase to liver fibrosis and liver cancer, oral squamous cell carcinoma, endothelial cell
It grows and migrates, the growth of colon cancer nude mice ascites tumor LOVO cell, osteosarcoma (osteosarcoma) etc. play inhibiting effect.Synthesis
Fumagillin derivatives (TNP-470, PPI-2458, CKD-732) have been used for treatment human cancer clinical test, by with
Methionine aminopeptidase 2 is that action target destroys tumor vessel；TNP-410 is for treating prostate cancer (prostate
Cancer), the cancers such as non-small cell lung cancer (NSCLC) and melanoma (melanoma).The neurotoxicity of fumidil is certain
Limit its application in clinical diagnosis in degree, and newfound fumagillin derivatives PPI-2458 and CKD-732 table
Lighter neurotoxicity is revealed.Fumidil and its derivative TNP-470 and CKD-732 can be reduced food absorption, weight, rouge
The size of fat amount and fat cell, thus the potential drug as treatment obesity.Fumidil can also effectively inhibit Ah meter
Bar disease (amebiasis) and other protozoans parasitize, and it is a variety of to can be used as treatment rheumatic arthritis and obesity etc.
The potential drug of disease.
Fumidil can be used for anti-microsporidian infection, have prevention and treatment Nosema apis parasitosis especially in apiculture
Effect, is also used for Microspora Infecting Fish treatment of infection.Honeybee sporidiosis has generation in developed country of respectively raising bees, the world, be by
Honeybee sporozoite colonizes in a kind of honeybee adult parasitic disease caused by honeybee enterocyte.Intestines intestines in microsporidian destruction
Wall opens road for the invasion of other microorganisms, microsporidiosis it is long along with Malpighian tube amoebiasis, chronic paralysis,
Black queen bee virus, spirochetosis and chalk disease etc., form so-called " Pafeng disease " mixed infection.Worker bee, queen bee and drone are all
Honeybee sporidiosis can be infected, causes body function weak, gathering honey ability reduces, the lost of life.Nosema apis parasitosis may
Delaying just makes entire bee colony dead for several years.Fumidil be can be successfully applied to prevention and treatment Nosema apis parasitosis, though it is not killed
Microsporidian spore, but the generation of Nosema apis can be greatly reduced, the infection rate that honeybee is total in bee colony is reduced, to reduce bee
The loss of group, increases the quantity of honeybee and larva, increases production honey.
The optimization culture method of secondary metabolite fumidil and its derivative is generated about aspergillus fumigatus, only seldom
Report (Wen Yang, Carbon and nitrogen source nutrition of fumagillin
Biosynthesis by Aspergillus fumigatus, Current Microbiology, 2003,275-279), but should
Fumidil its low output in report, is difficult to industrialized production or application.
Summary of the invention
The present invention provides a kind of methods using aspergillus fumigatus production fumidil (fumagillin), for influence cigarette
The carbon nitrogen source and microelement of aspergillus growth and fumidil are screened, and are carried out to corresponding microelement concentration excellent
Change, obtains higher fumidil yield.
The present invention provides a kind of method using aspergillus fumigatus production fumidil, which comprises the steps of:
The aspergillus fumigatus is inoculated in GMM solid medium and carries out actication of culture, the GMM Medium's PH Value is 6.5；
It is prepared by the strain spore after activation be inoculated in after spore suspension CYA solid medium, CXMA solid medium or
LMM fluid nutrient medium, the CYA solid medium, the CXMA solid medium pH value are naturally, the LMM fluid nutrient medium
PH value is adjusted to 5-7, cultivates under the conditions of 25 DEG C to 37 DEG C；
The aspergillus fumigatus and culture medium for collecting culture extract fumidil and fumagillin derivatives.
In the above-mentioned technical solutions, described every liter of GMM solid medium includes: glucose 10g, nitrate mother liquor 50mL,
Microelement mother liquor 1mL, yeast extract 1g, agar 15-20g, surplus is distilled water.
In the above-mentioned technical solutions, described every liter of CYA solid medium includes: NaNO32-5g, K2HPO4·3H2O
0.5-2g, MgSO4·7H2O 0.3-1g, KCl 0.3-1g, FeSO4·7H2O 0.01-0.03g, sucrose 20.0-40.0g, ferment
Female extract 3-8g, agar 15-20g, surplus are distilled water.
In the above-mentioned technical solutions, described every liter of CXMA solid medium includes: MgSO4·7H2O 0.3-0.5g, KCl
0.3-0.8g, FeSO4·7H2O 0.01-0.02g, xylan 20-40g, mannose 30-80g, glutamic acid 5-12g, agar 15-
20g, surplus are distilled water.
In the above-mentioned technical solutions, described every liter of LMM fluid nutrient medium includes: glucose 10g, yeast extract 10g, micro-
Secondary element mother liquor 1mL, nitrate mother liquor 50mL, surplus is distilled water.
In the above-mentioned technical solutions, described every liter of nitrate mother liquor includes: NaNO3120g, KCl 10.4g, MgSO4·
7H2O 10.4g, K2HPO4·3H2O 30.4g, surplus are distilled water.
In the above-mentioned technical solutions, the every 100mL of microelement mother liquor includes ZnSO4·7H2O 2.2g, H3BO3
1.1g, MnCl2·4H2O 0.5g, FeSO4·7H2O 0.16g, CoCl2·5H2O 0.16g, CuSO4·5H2O 0.16g,
(NH4)6Mo7O24·4H2O 0.11g, Na2EDTA 5.0g, surplus are distilled water.
In the above-mentioned technical solutions, the aspergillus fumigatus is that Aspergillus fumigatus af293 (can pass through business
Approach is commercially available).
In the above-mentioned technical solutions, the structural formula of the fumidil is as shown in Equation 1.
In the above-mentioned technical solutions, the fumagillin derivatives include fumagiringillin, described
The structural formula of fumagiringillin is as shown in Equation 2, further includes RK-95113, the structural formula of the RK-95113 such as 3 institute of formula
Show.
The present invention is using the method for aspergillus fumigatus production fumidil by selecting suitable culture medium prescription and culture item
Part substantially increases the yield of fumidil and obtains part fumagillin derivatives, is the industrialized production of fumidil
And application is laid a good foundation, and is also fumidil and its derivative before preparing neovascularization inhibitor, neovascularization inhibitor
Body compound, anti-tumor drug or its precursor compound, treatment amcbiasis and the drug that parasitizes of other protozoans or
Application in terms of the drug of preparation treatment rheumatic arthritis and obesity provides precondition.
Detailed description of the invention
Fig. 1 is the HPLC analysis detection figure of fumidil.
Specific embodiment
Below in conjunction with drawings and examples, a specific embodiment of the invention is described in more details, so as to energy
The advantages of enough more fully understanding the solution of the present invention and its various aspects.However, specific embodiments described below and reality
It applies example to be for illustrative purposes only, rather than limiting the invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1
Strain activation and culture base used in the present invention is GMM culture medium, and solid medium described in every 1L is matched as follows
System: glucose 10g, nitrate mother liquor 50mL, microelement mother liquor 1mL, yeast extract 1g, agar 15-20g, mentioned component
It successively adds, finally adds distilled water final volume to 1L, pH value is adjusted to 6.5,121 DEG C high pressure moist heat sterilization 20-30 minutes.?
90mm plate, streak inoculation, 37 DEG C stationary culture 3 days to 4 days.Aqua sterilisa collects spore, and end is made in each flat panel collector spore
Volume is the spore suspension of 2mL, and 4 DEG C save for cultivating inoculation.
Wherein, nitrate mother liquor (1L) method is as follows: sodium nitrate NaNO3120g, potassium chloride (KCl) 10.4g, sulfuric acid
Magnesium MgSO4·7H2O 10.4g, dipotassium hydrogen phosphate K2HPO4·3H2O 30.4g, mentioned component successively add, and finally add distillation
Water final volume to 1L, pH value naturally, 121 DEG C high pressure moist heat sterilization 20-30 minutes, room temperature preservation；
Microelement mother liquor (100mL) method is as follows: zinc sulfate ZnSO4·7H2O 2.2g, boric acid H3BO31.1g
Manganese chloride MnCl2·4H2O 0.5g, ferrous sulfate FeSO4·7H2O 0.16g, cobalt chloride CoCl2·5H2O 0.16g, copper sulphate
CuSO4·5H2O 0.16g, ammonium molybdate (NH4)6Mo7O24·4H2O 0.11g, EDETATE SODIUM salt Na2EDTA 5.0g, mentioned component according to
Secondary addition, finally adds distilled water final volume to 100mL, 121 DEG C high pressure moist heat sterilization 20-30 minutes, room temperature preservation.
Embodiment 2
Fermentation medium used is CYA culture medium, and CYA solid medium described in every 1L is prepared as follows: nitric acid
Sodium NaNO32-5g, dipotassium hydrogen phosphate K2HPO4·3H2O 0.5-2g, magnesium sulfate MgSO4·7H2O 0.3-1g, potassium chloride (KCl)
0.3-1g, ferrous sulfate FeSO4·7H2O 0.01-0.03g, sucrose 20.0-40.0g, yeast extract 3-8g, agar 15-
20g, mentioned component successively add, and finally add distilled water final volume to 1L, pH value is natural.121 DEG C of high pressure moist heat sterilizations 30 divide
Clock, the plate of falling 90mm, streak inoculation.The cultivation temperature is 25 to 37 DEG C, and culture medium is solid medium, stationary culture, training
Supporting the time is 4 days to 6 days.
It detects calculated by peak area method combination actual separation calculation method by HPLC fingerprint map analyzing to measure: every liter
It is about 34-50mg that CYA culture medium, which obtains fumidil yield,.
Embodiment 3
Fermentation medium used is CXMA culture medium, and solid medium described in every 1L is prepared as follows: magnesium sulfate
MgSO4·7H2O 0.3-0.5g, potassium chloride (KCl) 0.3-0.8g, ferrous sulfate FeSO4·7H2O 0.01-0.02g, xylan
20-40g, mannose 30-80g, glutamic acid 5-12g, agar 15-20g.Mentioned component successively adds, and finally adds distilled water end
For volume to 1L, pH value is natural.121 DEG C high pressure moist heat sterilization 30 minutes, the plate of falling 90mm, streak inoculation.The cultivation temperature is
25 DEG C to 37 DEG C, culture medium is solid medium, and stationary culture, incubation time is 4 days to 6 days.
It detects calculated by peak area method combination actual separation calculation method by HPLC fingerprint map analyzing to measure: every liter
It is about 25-35mg that CXMA culture medium, which obtains fumidil yield,.
Embodiment 4
Fermentation medium used is LMM culture medium, and fluid nutrient medium described in every 1L is prepared as follows: glucose
10g, yeast extract 10g, microelement mother liquor 1mL, nitrate mother liquor 50mL.Mentioned component successively adds, and finally adds steaming
Distilled water final volume to 1L, pH value is adjusted to 6.5.The wherein ingredient and preparation method of microelement mother liquor and nitrate mother liquor and implementation
It is identical in example 1.121 DEG C high pressure moist heat sterilization 30 minutes, the culture medium of falling 20mL to 90mm plate, every plate is inoculated with 0.2-0.5mL spore
Sub- suspension, shakes up.The cultivation temperature is 25 DEG C to 37 DEG C, and culture medium is fluid nutrient medium, stationary culture, incubation time 4
It was to 6 days.
It detects calculated by peak area method combination actual separation calculation method by HPLC fingerprint map analyzing to measure: every liter
It is about 50-60mg that LMM culture medium, which obtains fumidil yield,.
Embodiment 5
The HPLC analyzing detecting method of fermented and cultured product in above-described embodiment are as follows: be divided into the culture of 1 plate small
Block is placed in triangular flask, and it is (super that 20-40mL MEA (ethyl acetate: methanol: glacial acetic acid=89:10:1) progress ultrasonic extraction is added
Acoustic frequency 80Hz, time are 1 hour)；Collection extracting solution progress rotary evaporation (30 DEG C of temperature, revolving speed 100rpm, vacuum degree <
3mmHg)；It being dissolved after being evaporated with 0.30-0.60mL methanol, dilution debita spissitudo sample introduction 20-40ul, 20-100% methanol rushes column,
It carries out HPLC to analyze and identify, qualification result is as shown in Fig. 1, wavelength 350nm, and 15 minutes compounds of appearance time are that cigarette is bent
Mycin (fumagillin).
Embodiment 6
The HPLC isolation and purification method of fermented and cultured product in above-described embodiment are as follows: by multiple plate (3L of mass propgation
Culture medium) culture be divided into fritter and be placed in larger glass container, be added 3-5L MEA (ethyl acetate: methanol: glacial acetic acid=
Ultrasonic extraction (supersonic frequency 80Hz, time are 1 hour) 89:10:1) is carried out, extracting solution is collected, repeats aforesaid operations 3 times；By 3
Secondary extract liquor merges, and carries out rotary evaporation (30 DEG C of temperature, revolving speed 100rpm, vacuum degree < 3mmHg), obtains 3g coarse extract；By 3g
Coarse extract dissolved with methanol carry out HPLC prepare (RP-18, YMC-Pack, 250 × 10mm Column, 5 μm, flow velocity 3ml/
Min), chromatographic column addition reverse phase silica gel (ODS) in selection, mobile phase are first alcohol and water, and volume ratio is 20:80-100:0 methanol ladder
Degree elution.
Product through separation preparation is the compound fumidil (fumagillin) that formula 1 indicates:
The bacterium and the cultural method also can produce 3 table of fumagillin derivatives fumagiringillin and formula of the expression of formula 2
The fumagillin derivatives RK-95113 shown；
It finally, it should be noted that the above embodiment is merely an example for clearly illustrating the present invention, and is not pair
The restriction of embodiment.For those of ordinary skill in the art, it can also be made on the basis of the above description
Its various forms of variation or variation.There is no necessity and possibility to exhaust all the enbodiments.And it thus amplifies out
Obvious changes or variations be still in the protection scope of this invention.
Claims (4)
1. a kind of method using aspergillus fumigatus production fumidil, which comprises the steps of:
The aspergillus fumigatus is inoculated in GMM solid medium and carries out actication of culture, adjusting the GMM Medium's PH Value is 5-7；
CYA solid medium, CXMA solid medium or LMM liquid are inoculated in after strain spore after activation is prepared spore suspension
Body culture medium, the CYA solid medium, the CXMA solid medium pH value are naturally, the LMM fluid nutrient medium pH value tune
Section is 5-7, is cultivated under the conditions of 25 DEG C to 37 DEG C；
The aspergillus fumigatus and its culture medium for collecting culture extract fumidil and fumagillin derivatives；
Described every liter of GMM solid medium includes: glucose 10g, nitrate mother liquor 50mL, microelement mother liquor 1mL, and yeast is taken out
Extract 1g, agar 15-20g, surplus are distilled water,
Described every liter of CYA solid medium includes: NaNO32-5g, K2HPO4·3H2O 0.5-2g, MgSO4·7H2O 0.3-
1g, KCl 0.3-1g, FeSO4·7H2O 0.01-0.03g, sucrose 20.0-40.0g, yeast extract 3-8g, agar 15-20g,
Surplus is distilled water,
Described every liter of CXMA solid medium includes: MgSO4·7H2O 0.3-0.5g, KCl 0.3-0.8g, FeSO4·7H2O
0.01-0.02g, xylan 20-40g, mannose 30-80g, glutamic acid 5-12g, agar 15-20g, surplus are distilled water,
It includes: glucose 10g, yeast extract 10g, microelement mother liquor 1mL, nitric acid that the LMM fluid nutrient medium, which is every liter,
Salt mother liquor 50mL, surplus are distilled water,
Described every liter of nitrate mother liquor includes: NaNO3120g, KCl 10.4g, MgSO4·7H2O 10.4g, K2HPO4·3H2O
30.4g, surplus are distilled water,
The every 100mL of microelement mother liquor includes ZnSO4·7H2O 2.2g, H3BO31.1g, MnCl2·4H2O 0.5g,
FeSO4·7H2O 0.16g, CoCl2·5H2O 0.16g, CuSO4·5H2O 0.16g, (NH4)6Mo7O24·4H2O 0.11g,
Na2EDTA 5.0g, surplus are distilled water.
2. the method as described in claim 1, which is characterized in that the aspergillus fumigatus is Aspergillus fumigatus
af293。
3. the method as described in claim 1, which is characterized in that the structural formula of the fumidil is as shown in Equation 1.
4. the method as described in claim 1, which is characterized in that the fumagillin derivatives include fumagiringillin,
The structural formula of the fumagiringillin is as shown in Equation 2, further includes RK-95113, the structural formula of the RK-95113 such as formula 3
It is shown.
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| CN109722389B (en) * | 2017-10-31 | 2021-05-14 | 鲁南新时代生物技术有限公司 | Aspergillus fumigatus liquid culture medium |
| CN108165590B (en) * | 2017-12-20 | 2021-11-23 | 河南科技学院 | Culture medium and culture method for producing fumagillin by fermenting aspergillus fumigatus |
| CN109053638B (en) * | 2018-10-13 | 2020-07-31 | 浙江海正药业股份有限公司 | Extraction and purification method of fumagillin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1179715A (en) * | 1995-03-27 | 1998-04-22 | 萨诺费公司 | Use of fumagillol and derivatives thereof for preparing medicaments against intestinal infections |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1179715A (en) * | 1995-03-27 | 1998-04-22 | 萨诺费公司 | Use of fumagillol and derivatives thereof for preparing medicaments against intestinal infections |
Non-Patent Citations (3)
| Title |
|---|
| Carbon and Nitrogen Source Nutrition of Fumagillin Biosynthesis by Aspergillus fumigatus;Wen Yang;《Current Microbiology》;20030624;第46卷;第275-279 * |
| 三峡库区烟曲霉IMB-SX28次级代谢产物的分离及活性初步研究;王胤;《中国优秀硕士学位论文全文数据库》;20110915(第9期);第E079-13页 * |
| 烟曲霉素抑制小鼠结直肠癌生长及转移;崔晓楠;《中国癌症杂志》;20100228;第20卷(第2期);摘要 * |
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