CN106591150B - Selenium-rich cat Isaria culture and its cultural method - Google Patents
Selenium-rich cat Isaria culture and its cultural method Download PDFInfo
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Abstract
The present invention relates to a kind of selenium-rich cat Isaria culture and its cultural method, solve the problems, such as to add organic selenium in cat Isaria.The cultural method is with millet 800-900 parts, 35-50 parts of glucose, 0.06-0.14 parts of sodium selenite are made culture medium, cat Isaria strain are then inoculated on the culture medium again, after the completion of culture, mycelia and culture medium are placed in together to be dried in container, dries into mycelia block in two times.Inorganic selenium is added in culture medium during fungal culture by the present invention, by the fermentation of microorganism, inorganic selenium is made to become the organic selenium for being easier to be absorbed by organisms, the physiological activity and absorptivity of selenium not only can be improved, but also can reduce its toxicity.People increase the supplement of selenium element while taking artificial aweto, so that manually culture cordyceps sinensis increases the supplementary functions of selenium.
Description
Technical field
The present invention relates to cat Isaria culture and its cultural methods, especially in the cat stick for being wherein added to trace elements of selenium
Beam spore culture and its cultural method.
Background technique
Cordyceps sinensis is nourishing rare traditional Chinese medicine, nature and flavor sweet, warm.Function invigorating the lung and the kidney, preventing phlegm from forming and stopping coughing.Chronic cough can be used for
The illness of " void " such as void are breathed heavily, and puerperal asthenia, impotence are sombre.Cordyceps sinensis mainly contains Cordycepin, cordycepic acid, adenosine and more
The ingredients such as sugar；Cordycepin can inhibit the growth of the germs such as streptococcus, Actinobacillus mallei bacillus anthracis, and be the active matter of anticancer
Matter, the adjustment effect that endocrine system and nervous system to human body have had；Cordycepic acid can change human body cordyceps sinensis microcirculation,
With apparent reducing blood lipid and Antitussive and Expectorant Effect；Cordyceps sinensis polysaccharide is immunomodulator, can enhance body to virus and helminth
Resistance.
Cat Isaria is isolated bacterial strain from natural cordyceps cordycep, can be used as cordyceps sinensis after culture
Artificial substituting product, by China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation (deposit number: CGMCC
NO.0706), deuteromycetes, Moniliales, Isaria category are belonged in classification.
Selenium element is the essential trace elements of the human body, it plays important adjustment effect to the eubolism of human body, can be clear
Except free radical, anti-oxidant, strength inhibit the generation, enhancing immune function (20 times to 30 times), protection biology of lipid peroxide thin
After birth, prevents blood clot, removes cholesterol enhancing prostate regulatory function, has effect identical with insulin, can obviously promote
The cancer for luring carcinous elimination vivo mutations of skin aging, reduction carcinogen is prevented into the ability of cellular uptake sugar, blood circulation
Cell, the division for preventing cancer cell and growth etc..Selenium deficiency will lead to body immunity decline, cancer, cardiovascular disease, hepatopathy, white
Cataract or glaucoma, pancreatic disease, diabetes, genital system diseases etc. are all related with selenium deficiency.But selenium excess intake can produce body anxious, slow
Property toxic effect, while various toxic side effects such as genetoxic for selenium (sodium selenite) occur, inorganic selenium sodium selenite
Toxicity is bigger.
Summary of the invention
The present invention provides a kind of selenium-rich cat Isaria culture and its cultural method, at least to solve to add in cat Isaria
The problem of adding organic selenium.
In order to solve the above technical problems, according to an aspect of the invention, there is provided a kind of training of selenium-rich cat Isaria
The method of supporting, comprising steps of
Preparing culture medium, culture medium prescription component includes 800-900 parts of millet, and 35-50 parts of glucose, sodium selenite
0.06-0.14 parts；First be heated to boil by glucose is soluble in water, add millet and sodium selenite, continue heating boil to
Millet dries in the air to room temperature, sterilizes spare at stopping heating when solid-state；
Inoculation, cat Isaria mycelia are placed in 20 ± 2 DEG C as strain, cultivate in the ventilated environment of relative humidity 45-50%,
Wait cultivate completely after take out it is spare, cat Isaria select deposit number be CGMCC NO.0706 bacterial strain；
It dries, the mycelia after cultivating completely and culture medium is placed in dries in container together, dries into mycelia block in two times,
First day 95-110 DEG C of roasting 5h；The 2d days 80-90 DEG C of roasting 6h.
Further, culture medium prescription component includes 840 parts of millet, 42 parts of glucose, 0.07-0.14 parts of sodium selenite.
Further, the mycelia block dried, which is crushed into powder, last is made capsule.
Further, the method for the invention comprising steps of
Add water in casserole and glucose is added, is heated to boil, millet and sodium selenite aqueous solution is added, is covered after stirring evenly
Pot cover is stirred with scoop after 3min, is continued heating and is boiled 15-20 minutes, and constantly stir, closes when millet is at solid posture
Fire is placed into room temperature；
Culture medium is divided into fritter with scoop, is sub-packed in bottle, is sealed with brown paper block, and tightly wrapped with cotton cord；
Culture medium is placed in pressure cooker, totally 2 layers；Transfer needle is placed in Boiling tube and seals, and brown paper is fitted into envelope and seals
Mouthful, twined with cotton cord；It keeps test tube mouth upward, is placed in the culture medium gap put well；121 DEG C, 40min high pressure sterilization, go out
It is put in super-clean bench after bacterium, it is spare.
Inoculated and cultured 10-20 days, the good cat Isaria mycelia of growth conditions are complete to culture medium as strain for selection
It is cooling, it is inoculated with after ultraviolet disinfection 30min；
After inoculation, it is overlapped one layer of apertured plastic film in the culture medium brown paper side of covering, and tightly wrapped with cotton cord, will be inoculated with
Good culture medium is placed in 20 ± 2 DEG C, cultivates in the ventilated environment of humidity 45-50%；
Entire culture medium is covered with to mycelia, it is complete to be considered as culture；
After culture completely, mycelia in bottle and culture medium are taken out together and stirred evenly, rickle is uniformly layered on stainless steel disc；
Mycelia and culture medium are placed in drying box and dry into mycelia block, the 1st day 95-110 DEG C of roasting 5h；2nd day 80-90
DEG C roasting 6h；
It removes and dries mycelia block, break into medicinal powder with pulverizer；
It is packed into capsule, bottling sealing, shady place storage.
According to another aspect of the present invention, a kind of selenium-rich cat Isaria culture being prepared according to above method is provided
Object.
Inorganic selenium is added in culture medium during fungal culture by the present invention, by the fermentation of microorganism, makes inorganic selenium
Become the organic selenium for being easier to be absorbed by organisms, the physiological activity and absorptivity of selenium not only can be improved, but also it can be reduced
Toxicity.People increase the supplement of selenium element while taking artificial aweto, so that manually culture cordyceps sinensis increases the benefit of selenium
Fill function.
Detailed description of the invention
Fig. 1 is HPLC-ICP-MS testing result.
Specific embodiment
Organic selenium is converted by inorganic selenium using biological method, the physiological activity and absorptivity of selenium not only can be improved, and
And its toxicity can also be reduced.For this purpose, the cultural method of a kind of selenium-rich cat Isaria of the present invention and according to the cultural method
Preparation selenium-rich cat Isaria obtained.
In a kind of typical embodiment of the invention, a kind of cultural method of selenium-rich cat Isaria, including step system
Standby culture medium is inoculated with and dries, wherein
Culture medium, culture medium prescription component include 800-900 parts of millet, and 35-50 parts of glucose, sodium selenite 0.07-
0.14 part；First be heated to boil by glucose is soluble in water, add millet and sodium selenite, continue heating boil to millet at
Stop heating when solid-state, dries in the air to room temperature, sterilize spare；
Inoculation, cat Isaria mycelia are placed in 20 ± 2 DEG C as strain, cultivate in the ventilated environment of relative humidity 45-50%,
Wait cultivate completely after take out it is spare, cat Isaria select deposit number be CGMCC NO.0706 bacterial strain；
It dries, the mycelia after cultivating completely and culture medium is placed in dries in container together, dries into mycelia block in two times,
First day 95-110 DEG C of roasting 5h；The 2d days 80-90 DEG C of roasting 6h.
Culture medium is made by the above method for sodium selenite, using cat Isaria as Spawn incubation,
Inorganic selenium sodium selenite is converted to organic selenium with biological method.Experiment shows: the conversion ratio of selenium element can achieve 20% with
On.The physiological activity and absorptivity of selenium element are all improved, and the toxicity of selenium further decreases.Organic selenium and cat in culture
Isaria not only remains respective active constituent, more crucially has the effect of synergy, further enhances machine
Body immunity.
Preferably, culture medium prescription component includes 840 parts of millet, 42 parts of glucose, 0.06-0.14 parts of sodium selenite, can
More effectively to realize desired effect.
Preferably, the mycelia block dried be crushed into powder it is last capsule is made, convenient for taking.Instructions of taking: once a day, often
Secondary 0.3-1.0 grams, one after each meal.
In a kind of opposite specific embodiment, comprising steps of
Add water in casserole and glucose is added, is heated to boil, millet and sodium selenite aqueous solution is added, is covered after stirring evenly
Pot cover is stirred with scoop after 3min, prevents viscous pot, is continued heating and is boiled 15-20 minutes, and constantly stir, prevents from being burned, to
Millet is placed into room temperature at solid posture Shi Guanhuo.
Culture medium is divided into fritter with scoop, is sub-packed in bottle, bottling amount is 3/4.This process should be noted: try not to touch
To bottleneck, if any encountering, it is necessary to clean out.Then it is sealed with brown paper block, and is tightly wrapped with cotton cord.This process is it is noted that ox-hide
Paper quality, no leakage is without broken.
Culture medium is placed in pressure cooker, totally 2 layers；Transfer needle is placed in Boiling tube and seals, and brown paper is fitted into envelope and seals
Mouthful, twined with cotton cord；It keeps test tube mouth upward, is placed in the culture medium gap put well；121 DEG C, 40min high pressure sterilization, go out
It is put in super-clean bench after bacterium, it is spare.
Choosing inoculated and cultured 10-20 days, the inoculated and cultured is carried out in culture medium of the present invention, growth conditions
Good cat Isaria mycelia cools down completely as strain, to culture medium, is inoculated with after ultraviolet disinfection 30min.
Both hands are cleaned with cotton ball soaked in alcohol, light alcolhol burner, then dismantle the cotton cord of culture medium bottle mouth position winding, alcolhol burner flame
Open strain bottle cap and the test tube cap equipped with transfer needle in place.One people is semi-open by culture medium ox-hide paper cap, 45 ° of bottleneck inclinations, and one
People holds transfer needle and takes strain, after taking-up, is inoculated on culture medium with the method many places turn-taked immediately, covers culture base lid.One by one
Inoculation.
After inoculation, it is overlapped one layer of apertured plastic film in the culture medium brown paper side of covering, and tightly wrapped with cotton cord.It will inoculation
Good culture medium is put in large, indicates the inoculation date.20 ± 2 DEG C are placed in, is cultivated in the ventilated environment of humidity 45-50%；
Entire culture medium is covered with to mycelia, is considered as culture completely, this process needs 60-70 days.
After culture completely, mycelia in bottle and culture medium are taken out together and stirred evenly, rickle is uniformly layered on stainless steel disc.
Mycelia and culture medium are placed in drying box and dry into mycelia block, the 1st day 95-110 DEG C of roasting 5h；2nd day 80-90
DEG C roasting 6h, it has unexpectedly been found that, using drying twice, it is more advantageous to the pharmaceutical activity for playing cat Isaria and improves the absorption of selenium
Rate.
It removes and dries mycelia block, break into medicinal powder with pulverizer；
It is packed into capsule, bottling sealing, shady place storage.
In an exemplary embodiment of the invention, the selenium-rich cat Isaria being prepared using the above method is also provided
Culture.The culture is used to supplement the selenium element of needed by human body, while having the function of cat Isaria, i.e. those skilled in the art
The function of cordyceps sinensis known to member.
Preparation method and its having in terms of converting inorganic selenium of the invention are further illustrated below in conjunction with specific embodiment
Beneficial effect, it is the bacterial strain of CGMCC NO.0706 that cat Isaria, which selects deposit number, in embodiment.
Embodiment 1
(1) culture medium
Formula: 840 parts of millet, 42 parts of glucose, 0.10 part of sodium selenite.It is water-soluble that sodium selenite adds water 100ml to be made into
Liquid.
Add water 2000ml in casserole and glucose is added, is heated to boil, millet and sodium selenite aqueous solution is added, stirs evenly
After cover pot cover, stirred after 3min with scoop, continue heating and boil 20 minutes, and constantly stir, to millet at solid posture
Shi Guanhuo is placed into room temperature.Culture medium is divided into fritter with scoop, is sub-packed in bottle, is sealed with brown paper block, and twined with cotton cord
Tightly.Culture medium is placed in pressure cooker, totally 2 layers；Transfer needle is placed in Boiling tube and seals, and brown paper is fitted into envelope and seals,
It is twined with cotton cord；It keeps test tube mouth upward, is placed in the culture medium gap put well；121 DEG C, 40min high pressure sterilization, put after sterilizing
It is spare in super-clean bench.
(2) it is inoculated with
Inoculated and cultured 15 days, the good cat Isaria mycelia of growth conditions are completely cold to culture medium as strain for selection
But, it is inoculated with after ultraviolet disinfection 30min.Both hands are cleaned with cotton ball soaked in alcohol, light alcolhol burner, then dismantle the winding of culture medium bottle mouth position
Cotton cord opens strain bottle cap and the test tube cap equipped with transfer needle at alcolhol burner flame.One people is semi-open by culture medium ox-hide paper cap,
45 ° of bottleneck inclinations, a people holds transfer needle and takes strain, after taking-up, is inoculated on culture medium, is covered with the method many places turn-taked immediately
Cultivate base lid.It is inoculated with one by one.After inoculation, it is overlapped one layer of apertured plastic film in the culture medium brown paper side of covering, and use cotton cord
It tightly wraps.Inoculated culture medium is put in large, indicates the inoculation date.22 DEG C are placed in, is trained in the ventilated environment of humidity 50%
It supports.Entire culture medium is covered with to mycelia, is considered as culture completely, this process needs 70 days.
(3) it dries
After culture completely, mycelia in bottle and culture medium are taken out together and stirred evenly, rickle is uniformly layered on stainless steel disc.By bacterium
Silk and culture medium, which are placed in drying box, dries into mycelia block, the 1st day 100 DEG C of roasting 5h；2nd day 85 DEG C of roasting 6h.
(4) powder and glue capsule are played
It removes and dries mycelia block, break into medicinal powder with pulverizer.It is packed into capsule, bottling sealing, shady place storage.
Embodiment 2
(1) culture medium
Formula: 800 parts of millet, 35 parts of glucose, 0.07 part of sodium selenite.It is water-soluble that sodium selenite adds water 100ml to be made into
Liquid.
First glucose is dissolved in 2000ml water and is heated to boil, adds millet and sodium selenite, continues heating and boils
To millet at stopping heating when solid-state, dries in the air to room temperature, sterilize spare.
(2) it is inoculated with
Cat Isaria mycelia is placed in 22 DEG C as strain, cultivates in the ventilated environment of relative humidity 45%, after cultivating completely
It takes out spare.
(3) it dries
By after culture completely mycelia and culture medium be placed in dry in container together, dry into mycelia block in two times, first
Its 110 DEG C of roasting 5h；The 2d days 80 DEG C of roasting 6h.
Embodiment 3
(1) culture medium
Formula: 900 parts of millet, 50 parts of glucose, 0.14 part of sodium selenite.It is water-soluble that sodium selenite adds water 100ml to be made into
Liquid.
First glucose is dissolved in 2000ml water and is heated to boil, adds millet and sodium selenite, continues heating and boils
To millet at stopping heating when solid-state, dries in the air to room temperature, sterilize spare.
(2) it is inoculated with
Cat Isaria mycelia is placed in 18 DEG C as strain, cultivates in the ventilated environment of relative humidity 50%, after cultivating completely
It takes out spare.
(3) it dries
By after culture completely mycelia and culture medium be placed in dry in container together, dry into mycelia block in two times, first
Its 95 DEG C of roasting 5h；The 2d days 90 DEG C of roasting 6h.
Embodiment 4
(1) culture medium
Formula: 840 parts of millet, 42 parts of glucose, 0.10 part of sodium selenite.It is water-soluble that sodium selenite adds water 100ml to be made into
Liquid.
Add water 2000ml in casserole and glucose is added, is heated to boil, millet and sodium selenite aqueous solution is added, stirs evenly
After cover pot cover, stirred after 3min with scoop, continue heating and boil 15 minutes, and constantly stir, to millet at solid posture
Shi Guanhuo is placed into room temperature.Culture medium is divided into fritter with scoop, is sub-packed in bottle, is sealed with brown paper block, and twined with cotton cord
Tightly.Culture medium is placed in pressure cooker, totally 2 layers；Transfer needle is placed in Boiling tube and seals, and brown paper is fitted into envelope and seals,
It is twined with cotton cord；It keeps test tube mouth upward, is placed in the culture medium gap put well；121 DEG C, 40min high pressure sterilization, put after sterilizing
It is spare in super-clean bench.
(2) it is inoculated with
Inoculated and cultured 20 days, the good cat Isaria mycelia of growth conditions are completely cold to culture medium as strain for selection
But, it is inoculated with after ultraviolet disinfection 30min.Both hands are cleaned with cotton ball soaked in alcohol, light alcolhol burner, then dismantle the winding of culture medium bottle mouth position
Cotton cord opens strain bottle cap and the test tube cap equipped with transfer needle at alcolhol burner flame.One people is semi-open by culture medium ox-hide paper cap,
45 ° of bottleneck inclinations, a people holds transfer needle and takes strain, after taking-up, is inoculated on culture medium, is covered with the method many places turn-taked immediately
Cultivate base lid.It is inoculated with one by one.After inoculation, it is overlapped one layer of apertured plastic film in the culture medium brown paper side of covering, and use cotton cord
It tightly wraps.Inoculated culture medium is put in large, indicates the inoculation date.20 DEG C are placed in, is trained in the ventilated environment of humidity 45%
It supports.Entire culture medium is covered with to mycelia, is considered as culture completely, this process needs 60 days.
(3) it dries
After culture completely, mycelia in bottle and culture medium are taken out together and stirred evenly, rickle is uniformly layered on stainless steel disc.By bacterium
Silk and culture medium, which are placed in drying box, dries into mycelia block, the 1st day 95 DEG C of roasting 5h；2nd day 80 DEG C of roasting 6h.
(4) powder and glue capsule are played
It removes and dries mycelia block, break into medicinal powder with pulverizer.It is packed into capsule, bottling sealing, shady place storage.
Embodiment 5
(1) culture medium
Formula: 870 parts of millet, 40 parts of glucose, 0.12 part of sodium selenite.It is water-soluble that sodium selenite adds water 100ml to be made into
Liquid.
Add water 2000ml in casserole and glucose is added, is heated to boil, millet and sodium selenite aqueous solution is added, stirs evenly
After cover pot cover, stirred after 3min with scoop, continue heating and boil 18 minutes, and constantly stir, to millet at solid posture
Shi Guanhuo is placed into room temperature.Culture medium is divided into fritter with scoop, is sub-packed in bottle, is sealed with brown paper block, and twined with cotton cord
Tightly.Culture medium is placed in pressure cooker, totally 2 layers；Transfer needle is placed in Boiling tube and seals, and brown paper is fitted into envelope and seals,
It is twined with cotton cord；It keeps test tube mouth upward, is placed in the culture medium gap put well；121 DEG C, 40min high pressure sterilization, put after sterilizing
It is spare in super-clean bench.
(2) it is inoculated with
Inoculated and cultured 10 days, the good cat Isaria mycelia of growth conditions are completely cold to culture medium as strain for selection
But, it is inoculated with after ultraviolet disinfection 30min.Both hands are cleaned with cotton ball soaked in alcohol, light alcolhol burner, then dismantle the winding of culture medium bottle mouth position
Cotton cord opens strain bottle cap and the test tube cap equipped with transfer needle at alcolhol burner flame.One people is semi-open by culture medium ox-hide paper cap,
45 ° of bottleneck inclinations, a people holds transfer needle and takes strain, after taking-up, is inoculated on culture medium, is covered with the method many places turn-taked immediately
Cultivate base lid.It is inoculated with one by one.After inoculation, it is overlapped one layer of apertured plastic film in the culture medium brown paper side of covering, and use cotton cord
It tightly wraps.Inoculated culture medium is put in large, indicates the inoculation date.20 DEG C are placed in, is trained in the ventilated environment of humidity 50%
It supports.Entire culture medium is covered with to mycelia, is considered as culture completely, this process needs 60 days.
(3) it dries
After culture completely, mycelia in bottle and culture medium are taken out together and stirred evenly, rickle is uniformly layered on stainless steel disc.By bacterium
Silk and culture medium, which are placed in drying box, dries into mycelia block, the 1st day 110 DEG C of roasting 5h；2nd day 90 DEG C of roasting 6h.
(4) powder and glue capsule are played
It removes and dries mycelia block, break into medicinal powder with pulverizer.It is packed into capsule, bottling sealing, shady place storage.
It is the methods and results of organic selenium detection below.
Purpose: compare the difference of organic selenium content between each sample
Experimental material: 1 selenium-rich cat Isaria hypha capsule of sample to be tested 1(embodiment), the non-selenium-rich worm of sample to be tested 2(
Grass), centrifuge tube, lipase, protease, ice cube, the organic filter membrane of 0.45um etc.
Instrument and equipment:
Reciprocating water-bath constant temperature oscillator: SHZ-88A type, granary experimental facilities factory, China.
Centrifuge: II type of CF15RX, Hitachi, Ltd, Japan.
High performance liquid chromatograph: 1200 types, agilent company, the U.S..
Icp ms: 2 series of X matches Mo Feishier company, the U.S..
Experimental method: weigh sample+enzyme → dissolution → water bath with thermostatic control oscillation → ice water rapidly cool down → centrifugation →
Supernatant is taken to cross film → HPLC-ICP-MS detection
Experimental result Fig. 1 and table 1.
Conversion ratio are as follows: (21.72+23.3+3.76)/228=21.39%.Calculation method: by every 840 grams of millets plus Asia selenium
Sour sodium 140mg is calculated, can output medicinal powder be 280 grams, i.e. the content of sodium selenite is 140 280=0.5mg of ÷ in every gram of product, sub-
The content of selenium is 45.6% in sodium selenate, is 0.5 × 0.456=0.228mg by this content for calculating inorganic selenium, has in institute's sample
The total content of machine selenium is 21.72+23.30+3.76=48.78, then the content of organic selenium are as follows: 48.78 ÷ 228=21.39%.
Claims (5)
1. a kind of preparation method of selenium-rich cat Isaria culture, it is characterised in that comprising steps of
Preparing culture medium, culture medium prescription component includes 800-900 parts of millet, and 35-50 parts of glucose, sodium selenite 0.06-
0.14 part；First be heated to boil by glucose is soluble in water, add millet and sodium selenite, continue heating boil to millet at
Stop heating when solid-state, dries in the air to room temperature, sterilize spare；
Inoculation, cat Isaria mycelia are placed in 20 ± 2 DEG C as strain, cultivate in the ventilated environment of relative humidity 45-50%, wait train
Taking-up is spare after supporting completely, and it is the bacterial strain of CGMCC NO.0706 that cat Isaria, which selects deposit number,；
Dry, by after culture completely mycelia and culture medium be placed in dry in container together, dry into mycelia block in two times, first
It 95-110 DEG C roasting 5h；2nd day 80-90 DEG C of roasting 6h obtains the selenium-rich cat Isaria culture.
2. the preparation method of selenium-rich cat Isaria culture according to claim 1, it is characterised in that: culture medium prescription group
Divide includes 840 parts of millet, 42 parts of glucose, 0.06-0.14 parts of sodium selenite.
3. the preparation method of selenium-rich cat Isaria culture according to claim 1 or 2, it is characterised in that: the bacterium dried
Silk block, which is crushed into powder, last is made capsule.
4. the preparation method of selenium-rich cat Isaria culture according to claim 3, it is characterised in that comprising steps of
Add water in casserole and glucose is added, is heated to boil, millet and sodium selenite aqueous solution is added, covers pot after stirring evenly
It covers, is stirred after 3min with scoop, continue heating and boil 15-20 minutes, and constantly stir, closed when millet is at solid posture
Fire is placed into room temperature；
Culture medium is divided into fritter with scoop, is sub-packed in bottle, is sealed with brown paper block, and tightly wrapped with cotton cord；
Culture medium is placed in pressure cooker, totally 2 layers；Transfer needle is placed in Boiling tube and seals, and brown paper is fitted into envelope and seals,
It is twined with cotton cord；It keeps test tube mouth upward, is placed in the culture medium gap put well；121 DEG C, 40min high pressure sterilization, put after sterilizing
It is spare in super-clean bench；
Inoculated and cultured 10-20 days, the good cat Isaria mycelia of growth conditions are completely cold to culture medium as strain for selection
But, it is inoculated with after ultraviolet disinfection 30min；
After inoculation, it is overlapped one layer of apertured plastic film in the culture medium brown paper side of covering, and tightly wrapped with cotton cord, it will be inoculated
Culture medium is placed in 20 ± 2 DEG C, cultivates in the ventilated environment of humidity 45-50%；
Entire culture medium is covered with to mycelia, it is complete to be considered as culture；After culture completely, mycelia in bottle and culture medium are taken out together and stirred
Even, rickle is uniformly layered on stainless steel disc；
Mycelia and culture medium are placed in drying box and dry into mycelia block, the 1st day 95-110 DEG C of roasting 5h；2nd day 80-90 DEG C it is roasting
6h；
It removes and dries mycelia block, break into medicinal powder with pulverizer；
It is packed into capsule, bottling sealing, shady place storage.
5. a kind of selenium-rich cat Isaria culture, it is characterised in that: method according to claim 1 or 2 or 4 is prepared into
It arrives.
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| CN201611163895.6A CN106591150B (en) | 2016-12-16 | 2016-12-16 | Selenium-rich cat Isaria culture and its cultural method |
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| CN201611163895.6A CN106591150B (en) | 2016-12-16 | 2016-12-16 | Selenium-rich cat Isaria culture and its cultural method |
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| CN106591150B true CN106591150B (en) | 2019-09-03 |
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