CN106590020B - Utilize the method and its application of macroporous absorbent resin separation water-soluble monascus pigment - Google Patents
Utilize the method and its application of macroporous absorbent resin separation water-soluble monascus pigment Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
Abstract
The invention discloses a kind of method and its application using macroporous absorbent resin separation water-soluble monascus pigment.This method is that the monascus ruber fermenation raw liquid that will be fermented in liquid state fermentation culture medium is separated by solid-liquid separation, take liquid, obtain extracellular water-soluble monascus pigment fermentation liquid, then it is added and is adsorbed by pretreated macroporous absorbent resin, it is eluted again with ethyl alcohol, vacuum freezedrying is carried out after obtained eluent removal eluant, eluent, finally obtains water-soluble monascus pigment.This method is that monascorubin is carried out purification & isolation using macroporous absorbent resin, solve the problems, such as that water colo(u)r is unstable and fermentation liquid in impurity it is more；It simultaneously by removing the impurity such as polysaccharide, inorganic salts in monascorubin fermentation liquid, realizes that extracellular monascorubin efficiently separates, has a good application prospect and market value.
Description
Technical field
It is the present invention relates to the separation method of monascorubin, in particular to a kind of water-soluble red using macroporous absorbent resin separation
The method and its application of bent pigment.
Background technique
Monascorubin is a kind of natural pigment, by Monascus fermenting and producing, is proposed from the red yeast rice of Fermentation Condition of Monascus spp
Pigment is mainly para chrome, and it is all non-water-soluble that these pigments, which are soluble in ethyl alcohol, the biggish organic solution of acetone isopolarity,
Pigment is subject to certain restrictions in terms of food applications.With going deep into for monascorubin research, produced in recent years with liquid fermentation
Monascorubin then there is good water solubility.Thermal stability, light shine, antioxidant and the resistance to reduction of water-soluble monascus pigment
The performance of agent is good, and developmental research has broad prospects and great economic benefit.But at present to water-soluble in Monas cuspurpureus Went fermentation liquid
Property pigment isolate and purify research report it is relatively fewer, efficiency is lower.Such as have been reported that spent ion exchange resin (D380,
D301R, 110H etc.) separation monascorubin, there is the problems such as being difficult to elution or not high eluting rate (Lian Xi army etc., food industry section
Skill, 2003,24 (8): 81-83)；It has been reported that and separates water-soluble monascus pigment with anion exchange resin D301, obtain purity very
High monascorubin, there are problems that all recycling pigment (what strong etc., Southwest University's journal natural science edition of bear,
2009,31(9):20-24)；It has been reported that with macroporous absorbent resin (X-5, AB-8, D14、D16Deng) separation monascorubin, exist back
The problems such as yield is relatively low (Tong Qunyi, Sichuan Light Chemical Engineering College's journal, 1999,12 (4): 9-12)；It has been reported that with macroreticular resin NKA
Deng processing monascorubin waste liquid, there is a problem of adsorption rate and eluting rate it is all relatively low (hold gorgeous skin of bamboo etc., Food Additives Used in China,
2012,01:154-157)。
Usually increase the generation that soluble protein, peptide, amino acid promote water-soluble monascus pigment in the medium,
Hassan Hajjaj etc. (International Journal of Food Science and Technology, 2015,50:
1731-1736) by optimizing carbon nitrogen source ratio and the extracellular monascorubin of fermentation condition increase in red koji fermentation culture medium,
But fermentation broth contents are become more complicated, red, orange, yellow three classes pigment and protein, polypeptide, amino acid, Glucosamine
Equal substances, which combine, generates a variety of derivatives.In addition, Chen gong etc. (BMC Biotechnology, 2015,15 (1): 72-80)
By single carbon source (glucose) progress high density fermentation of adding the extracellular uranidin of monascus liquid fermentation is increased significantly, but
Residual sugar is higher than 25g/L in finally obtained Monas cuspurpureus Went fermentation liquid, increases what later period water-soluble monascus pigment product was separated and purified
Therefore difficulty needs a kind of method for efficiently separating water-soluble monascus pigment.
Summary of the invention
The primary purpose of the present invention is that the shortcomings that overcoming the prior art and insufficient, provides and a kind of utilize macroporous absorbent resin
The method for separating water-soluble monascus pigment.
Water-soluble monascus pigment method is separated using macroporous absorbent resin another object of the present invention is to provide described
Using.
The purpose of the invention is achieved by the following technical solution: a kind of to separate water-soluble monascus color using macroporous absorbent resin
The method of element, includes the following steps:
(1) pretreatment of fermentation liquid: the good monascus ruber fermenation raw liquid of liquid state fermentation being separated by solid-liquid separation, liquid is taken,
Obtain extracellular water-soluble monascus pigment fermentation liquid；
(2) pretreatment of resin: macroporous absorbent resin is pre-processed, wet resin is obtained；
(3) it adsorbs: wet resin obtained in step (2) is added to extracellular water-soluble monascus color obtained in step (1)
It is adsorbed in plain fermentation liquid, is separated by solid-liquid separation, takes solid, obtain filter cake；
(4) it desorbs: filter cake obtained in step (3) is eluted with ethyl alcohol, be separated by solid-liquid separation, collect liquid, washed
De- liquid；
(5) it removes eluant, eluent: eluent obtained in step (4) being removed into eluant, eluent, obtains concentrate；
(6) it is dried in vacuo: concentrate obtained in step (5) being subjected to vacuum freezedrying, obtains water-soluble monascus color
Element.
The culture medium of liquid state fermentation described in step (1) is preferred are as follows: in every 100mL liquid state fermentation culture medium, contains grape
Sugar 10~30g, (NH4)2SO4 0.5g、KH2PO4 0.5g、MgSO4·7H2O 0.05g、FeSO4·7H2O 1.0mg、ZnSO4·
7H2O 1.0mg、MnSO4·H2O 3.0mg is settled to 100ml with water, and pH is natural.
The water is distilled water or deionized water.
Monascus ruber described in step (1) is preferably the monascus ruber that deposit number is CGMCC No.10910
(Monascus ruber)。
The color value of extracellular water-soluble monascus pigment fermentation liquid described in step (1) is 40~250AU350/ mL, preferably
99.13~135.88AU350/mL。
The method of separation of solid and liquid described in step (1), (3) and (4) preferably filters.
Macroporous absorbent resin described in step (2) is preferably macroporous absorbent resin DA201, more preferably macroporous absorption tree
Rouge DA201-B or DA201-C.
Macroporous absorbent resin pretreated method is carried out described in step (2) to be preferably achieved by the steps of:
(a) macroporous absorbent resin is impregnated with ethyl alcohol, then is rinsed with ethyl alcohol, be washed till the ethyl alcohol of outflow without muddiness, then use
It is washed to no alcohol taste；
(b) it is then impregnated, is washed with water to neutrality with HCl solution, then impregnated with NaOH solution, be washed with water to neutrality, obtain
Treated macroporous absorbent resin (wet resin)；
(c) to this termination；Or repeat step (b) 2~3 times.
The concentration of ethyl alcohol described in step (a) is preferably percent by volume 95%.
Described in step (a) with ethyl alcohol impregnate time be 8~for 24 hours, preferably 12h.
Water described in step (a) and step (b) is preferably distilled water or deionized water.
The concentration of HCl solution described in step (b) is preferably percent by volume 2~8%, preferably 4%.
Described in step (b) with HCl solution impregnate time be preferably 2~8h, preferably 4h.
The concentration of NaOH solution described in step (b) is preferably percent by volume 2~8%, preferably 4%.
Described in step (b) with NaOH solution impregnate time be preferably 2~8h, preferably 4h.
Obtaining that treated described in step (b), macroporous absorbent resin is saved with wet process.
Macroporous absorbent resin additive amount described in step (3) is to match by the extracellular water-soluble monascus pigment fermentation liquid of every mL
0.2~0.5g macroporous absorbent resin calculates, and preferably matches 0.43~0.5g by the extracellular water-soluble monascus pigment fermentation liquid of every mL
Macroporous absorbent resin calculates.
Absorption includes carrying out Static Adsorption by standing mode and being inhaled by way of oscillation described in step (3)
It is attached.
The time of the absorption is 0.15~2.5h, preferably 0.25~0.5h.
Elution described in step (4) is static elution or dynamic gradient elution.
The method of the static elution is preferably achieved by the steps of: the filter cake that will be obtained is fitted into container, is added
Ethyl alcohol, standing are eluted.
The container is preferably glass container, more preferably beaker.
The concentration of the ethyl alcohol is preferably percent by volume 95%.
The method of the dynamic gradient elution is preferably achieved by the steps of: obtained filter cake is packed into normal pressure chromatography
Column carries out gradient elution with the ethyl alcohol of the various concentration of 2 times of column volumes.
The concentration of the ethyl alcohol is percent by volume 25%, 50%, 75% and 100%.
The speed of the gradient elution is preferably 1~10BV/h；Preferably 5BV/h.
The time of the elution is 0.15~2.5h, preferably 0.25~0.5h.
The method that eluant, eluent is removed described in step (5) is preferably removed by reduction vaporization mode, while be can be recycled and be washed
De- agent, resource are reused.
The reduction vaporization carries out under the conditions of 45 DEG C of water-baths, vacuum degree≤- 0.085Mpa.
The method using macroporous absorbent resin separation water-soluble monascus pigment is in separation water-soluble monascus pigment
Application.
The present invention has the following advantages and effects with respect to the prior art:
1, the present invention can be obtained under Dynamic Adsorption elution using the method for macroporous absorbent resin separation water-soluble monascus pigment
It is real to high-recovery (95% or more) and the extracellular water-soluble monascus pigment of desalination (95% or more), desugar (98% or more)
Isolating and purifying for water-soluble monascorubin is showed, while the macroreticular resin DA201 that the present invention uses is to water-soluble monascus color
Rapid 0.25h~the 0.5h of adsorption-desorption process of element substantially can reach adsorption-desorption balance, this has industrial production wide
Application prospect.
2, the present invention is according to inorganic salts, polysaccharide, protein etc. between water-soluble monascus pigment and in Monas cuspurpureus Went fermentation liquid
Monascorubin is carried out purification & isolation using macroporous absorbent resin, solved water-soluble by the difference of each component molecules size in impurity
Pigment is unstable and fermentation liquid in problem more than impurity；It is miscellaneous by removing monascorubin polysaccharide of fermentation broth, inorganic salts etc. simultaneously
Matter realizes that extracellular monascorubin efficiently separates, and has a good application prospect and market value.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Embodiment 1 separates water-soluble monascus pigment using macroporous absorbent resin DA201-C
(1) pretreatment of fermentation liquid: the monascus ruber Monascusruber that will be fermented in liquid state fermentation culture medium
(CGMCC No.10910) fermentation liquid is filtered, and filtrate is taken, and is obtained extracellular water-soluble monascus pigment fermentation liquid, is measured extracellular water
The color value of soluble red rice red pigment is 99.13AU350/ mL is (with the extinction at ultraviolet specrophotometer measurement characteristic wavelength 350nm
Degree), salinity 2.15ms/cm (conductivity), residual sugar content 30g/L (DNS method), wherein the composition of liquid state fermentation culture medium is as follows:
Glucose 7.5g, (NH4)2SO40.25g, KH2PO40.25g, MgSO4·7H2O 0.025g, KCl 0.025g, FeSO4·
7H2O 0.5mg, ZnSO4·7H2O 0.5mg, MnSO4·H2O 1.5mg is settled to 50mL with distilled water, and pH is natural.
(2) pretreatment of resin: by the DA201-C macroreticular resin (Zhengzhou Qin Shi Science and Technology Ltd.) of purchase with 95%
(v/v) alcohol solution dipping 12h is rinsed with 95% (v/v) ethyl alcohol first, washes the ethyl alcohol of outflow without muddiness, then use distilled water
It is washed till no alcohol taste；Then 4h is impregnated with 4% (v/v) HCl solution, is washed to neutrality with distillation, then with 4% (v/v) NaOH solution
4h is impregnated, neutrality is washed to distillation, is repeated 2 times, wet process saves backup.
(3) adsorb: the extracellular water-soluble monascus pigment for taking 5mL step (1) to obtain is added treated in step (2)
DA201-C wet resin 2.5g, stirs evenly, standing adsorption 0.25h, filters, obtains filter cake, while the color value for measuring filtrate is
3.37AU350/mL。
(4) it desorbs: the filter cake that step (3) obtains is placed in a beaker, the elution of 95% (v/v) ethanol solution static state is added
0.25h, filter, collect eluent, measure its salinity be 0.25ms/cm (conductivity), residual sugar amount 3.37g/L (DNS method) and
Color value is 76.24AU350/ mL (with the absorbance at ultraviolet specrophotometer measurement characteristic wavelength 350nm).
(5) recycle eluant, eluent: the eluent that step (4) is obtained using reduction vaporization mode (45 DEG C of bath temperature, vacuum
Degree≤- 0.085MPa) recycling eluant, eluent, the concentrate for the eluant, eluent that is removed.
(6) be dried in vacuo: the concentrate that step (5) is obtained carry out vacuum freezedrying (- 20 DEG C of cryogenic temperature, vacuum
≤ -0.037Mpa), obtain water-soluble monascus pigment product.
As a result: to the adsorption rate of water-soluble monascus pigment in fermentation liquid up to 96.60%, pigment returns DA201-C macroreticular resin
Yield is 76.91%, and salt removal rate is 88.37%, and sugared removal rate is 88.77%.Show to utilize macroporous absorbent resin DA201-C
The high-purity water soluble red rice red pigment of available desalination, desugar.
Embodiment 2 separates water-soluble monascus pigment using macroporous absorbent resin DA201-B
(1) pretreatment of fermentation liquid: with step (1) in embodiment 1.
(2) pretreatment of resin: identical as embodiment 1 step (2) in sheet, difference is only that the present embodiment using DA201-
B (Zhengzhou Qin Shi Science and Technology Ltd.) macroreticular resin is instead of DA201-C macroreticular resin in embodiment 1.
(3) adsorb: the extracellular water-soluble monascus pigment for taking 5mL step (1) to obtain is added treated in step (2)
DA201-B wet resin 2.5g, stirs evenly, standing adsorption 30min, filters, obtains filter cake, while the color value for measuring filtrate is
0.88AU350/mL。
(4) it desorbs: the filter cake that step (3) obtains is placed in a beaker, the elution of 95% (v/v) ethanol solution static state is added
0.5h is filtered, and collects eluent, and measuring its salinity is 0.35ms/cm (conductivity), residual sugar amount 4.30g/L (DNS method) and color
Valence is 75.21AU350/ mL (with the absorbance at ultraviolet specrophotometer measurement characteristic wavelength 350nm).
(5) eluant, eluent is recycled: with 1 step of embodiment (5).
(6) it is dried in vacuo: with 1 step of embodiment (6).
As a result: DA201-B macroreticular resin can reach the adsorption rate of water-soluble monascus pigment in fermentation liquid after 0.5h
99.11%, pigment recovery rate is 75.87%, and salt removal rate is 83.72%, and sugared removal rate is 85.67%.Show to utilize macropore
Adsorb the water-soluble monascus pigment of the available desalination desugar of resin DA201-B.
Embodiment 3 separates water-soluble monascus pigment using macroporous absorbent resin DA201-C
(1) pretreatment of fermentation liquid: the monascus ruber Monascusruber that will be fermented in liquid state fermentation culture medium
(CGMCC No.10910) fermentation liquid is filtered, and supernatant is taken, and obtains extracellular water-soluble monascus pigment fermentation liquid, is measured extracellular
The color value of water-soluble monascus pigment is 135.88AU350/ mL is (with the extinction at ultraviolet specrophotometer measurement characteristic wavelength 350nm
Degree), salinity 2.37ms/cm (conductivity), residual sugar content 137.01g/L (DNS method), wherein the composition of liquid state fermentation culture medium
It is as follows: glucose 15g, (NH4)2SO40.25g, KH2PO40.25g, MgSO4·7H2O 0.025g, KCl 0.025g,
FeSO4·7H2O0.5mg, ZnSO4·7H2O 0.5mg, MnSO4·H2O 1.5mg is settled to 50mL with distilled water, and pH is natural.
(2) pretreatment of resin: with 1 step of embodiment (2).
(3) it adsorbs: taking treated DA201-C wet resin in 25g step (2), the born of the same parents that 58mL step (1) obtains are added
Outer water-soluble monascus pigment adsorbs 0.25h with 180rpm speed oscillation, filters, be washed to the clarification of water of outflow, obtain filter cake,
Color value (the 1.43AU of filtrate is measured simultaneously350/mL)。
(4) desorb: the filter cake that step (3) is obtained is packed into normal pressure chromatography glass column (Φ 15mm × 305mm), respectively with 2
The different concentration ethanol (25%, 50%, 75%, 100%, v/v) of times column volume, totally 8 times of column volume eluant, eluents (are successively used
25%, 50%, 75%, 100% ethanol elution) gradient elution is carried out, gradient elution speed is 5BV/h.Eluent is collected, is surveyed
Its fixed salinity is 0.1ms/cm (conductivity), residual sugar amount (DNS method) 2.36g/L and color value 129.55AU350/ mL is (with ultraviolet point
Absorbance at light photometric determination characteristic wavelength 350nm).
(5) eluant, eluent is recycled: with 1 step of embodiment (5).
(6) it is dried in vacuo: with 1 step of embodiment (6).
As a result: to the adsorption rate of water-soluble monascus pigment in fermentation liquid up to 98.95%, pigment returns DA201-C macroreticular resin
Yield is 95.34%, and salt removal rate is 95.78%, and sugared removal rate is 98.28%, shows to utilize macroporous absorbent resin DA201-C
The high-purity water soluble red rice red pigment product of available desalination, desugar.
Comparative example 1 separates water-soluble monascus pigment using macroporous absorbent resin D150
(1) pretreatment of fermentation liquid: with step (1) in embodiment 1.
(2) pretreatment of resin: identical as embodiment 1 step (2) in sheet, difference is only that the present embodiment using D150
(Zhengzhou Qin Shi Science and Technology Ltd.) macroreticular resin is instead of DA201-C macroreticular resin in embodiment 1.
(3) adsorb: the extracellular water-soluble monascus pigment for taking 5mL step (1) to obtain is added treated in step (2)
D150 wet resin 2.5g, stirs evenly, standing adsorption 30min, filters, obtains filter cake, while the color value for measuring filtrate is
38.30AU350/mL。
(4) it desorbs: the filter cake that step (3) obtains is placed in a beaker, the elution of 95% (v/v) ethanol solution static state is added
0.5h is filtered, and collects eluent, and measuring its salinity is 0.42ms/cm (conductivity), residual sugar amount 9.31g/L (DNS method) and color
Valence is 11.01AU350/ mL (with the absorbance at ultraviolet specrophotometer measurement characteristic wavelength 350nm).
(5) eluant, eluent is recycled: with 1 step of embodiment (5).
(6) it is dried in vacuo: with 1 step of embodiment (6).
As a result: D150 macroreticular resin can reach the adsorption rate of water-soluble monascus pigment in fermentation liquid after 0.5h
61.36%, pigment recovery rate is 11.11%, and salt removal rate is 80.47%, and sugared removal rate is 68.97%.Show to utilize macropore
It adsorbs resin D150 and separates water-soluble monascus pigment, although can achieve certain desalting effect, but obtained water solubility is red
Bent pigment recovery rate is extremely low.
Comparative example 2 separates water-soluble monascus pigment using macroporous absorbent resin LX-38
(1) pretreatment of fermentation liquid: with step (1) in embodiment 1.
(2) pretreatment of resin: identical as embodiment 1 step (2) in sheet, difference is only that the present embodiment using LX-38
(Zhengzhou Qin Shi Science and Technology Ltd.) macroreticular resin is instead of DA201-C macroreticular resin in embodiment 1.
(3) adsorb: the extracellular water-soluble monascus pigment for taking 5mL step (1) to obtain is added treated in step (2)
LX-38 wet resin 2.5g, stirs evenly, standing adsorption 30min, filters, obtains filter cake, while the color value for measuring filtrate is
8.61AU350/mL。
(4) it desorbs: the filter cake that step (3) obtains is placed in a beaker, the elution of 95% (v/v) ethanol solution static state is added
0.5h is filtered, and collects eluent, and measuring its salinity is 0.72ms/cm (conductivity), residual sugar amount 6.64g/L (DNS method) and color
Valence is 59.79AU350/ mL (with the absorbance at ultraviolet specrophotometer measurement characteristic wavelength 350nm).
(5) eluant, eluent is recycled: with 1 step of embodiment (5).
(6) it is dried in vacuo: with 1 step of embodiment (6).
As a result: LX-38 macroreticular resin can reach the adsorption rate of water-soluble monascus pigment in fermentation liquid after 0.5h
91.31%, pigment recovery rate is 60.31%, and salt removal rate is 66.51%, and sugared removal rate is 77.87%.Show to utilize macropore
Separation of the resin LX-38 to water-soluble monascus pigment, the water-soluble monascus pigment of available certain desugar are adsorbed, but is recycled
Rate and salt rejection rate be not high.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (9)
1. a kind of method using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that include the following steps:
(1) pretreatment of fermentation liquid: the good monascus ruber fermenation raw liquid of liquid state fermentation is separated by solid-liquid separation, liquid is taken, obtains
Extracellular water-soluble monascus pigment fermentation liquid；
(2) pretreatment of resin: macroporous absorbent resin is pre-processed, wet resin is obtained；
(3) it adsorbs: wet resin obtained in step (2) being added to extracellular water-soluble monascus pigment obtained in step (1) and is sent out
It is adsorbed in zymotic fluid, is separated by solid-liquid separation, takes solid, obtain filter cake；
(4) it desorbs: filter cake obtained in step (3) is eluted with ethyl alcohol, be separated by solid-liquid separation, collect liquid, obtain eluent；
(5) it removes eluant, eluent: eluent obtained in step (4) being removed into eluant, eluent, obtains concentrate；
(6) it is dried in vacuo: concentrate obtained in step (5) being subjected to vacuum freezedrying, obtains water-soluble monascus pigment；Step
Suddenly macroporous absorbent resin described in (2) is macroporous absorbent resin DA201.
2. the method according to claim 1 using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that:
Absorption includes carrying out Static Adsorption by standing mode and being adsorbed by way of oscillation described in step (3)；
Elution described in step (4) is static elution or dynamic gradient elution.
3. the method according to claim 2 using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that:
The method of the static elution is achieved by the steps of: the filter cake that will be obtained is fitted into container, and ethyl alcohol is added, and is stood
It is eluted；
The method of the dynamic gradient elution is achieved by the steps of: obtained filter cake being packed into normal pressure chromatographic column, with 2 times
The ethyl alcohol of the various concentration of column volume carries out gradient elution.
4. the method according to claim 2 using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that:
The time of the absorption is 0.15~2.5 h；
The time of the elution is 0.15~2.5 h.
5. the method according to claim 1 using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that:
Macroporous absorbent resin pretreated method is carried out described in step (2) to be achieved by the steps of:
(a) macroporous absorbent resin is impregnated with ethyl alcohol, then is rinsed with ethyl alcohol, be washed till the ethyl alcohol of outflow without muddiness, be then washed with water
To no alcohol taste；
(b) it is then impregnated, is washed with water to neutrality with HCl solution, then impregnated with NaOH solution, be washed with water to neutrality, handled
Macroporous absorbent resin afterwards；
(c) to this termination；Or repeat step (b) 2~3 times.
6. the method according to claim 5 using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that:
The concentration of ethyl alcohol described in step (a) is percent by volume 95%；
Described in step (a) with ethyl alcohol impregnate time be 8~24 h；
Water described in step (a) and step (b) is distilled water or deionized water；
The concentration of HCl solution described in step (b) is percent by volume 2~8%；
Described in step (b) with HCl solution impregnate time be 2~8 h；
The concentration of NaOH solution described in step (b) is percent by volume 2~8%；
Described in step (b) with NaOH solution impregnate time be 2~8 h.
7. the method according to claim 1 using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that:
Macroporous absorbent resin additive amount described in step (3) be by every mL extracellular water-soluble monascus pigment fermentation liquid proportion 0.2~
0.5g macroporous absorbent resin calculates.
8. the method according to claim 1 using macroporous absorbent resin separation water-soluble monascus pigment, it is characterised in that:
Monascus ruber as described in step (1) be monascus ruber that deposit number is CGMCC No. 10910 (Monascus
Ruber)；
The composition of the culture medium of liquid state fermentation as described in step (1) is as follows: in every 100 mL liquid state fermentation culture medium, containing grape
Sugar 10~30 g, (NH4)2SO4 0.5 g、KH2PO4 0.5 g、MgSO4·7H2O 0.05 g、FeSO4·7H2O 1.0 mg、
ZnSO4·7H2O 1.0 mg、MnSO4·H23.0 mg of O is settled to 100 ml with water, and pH is natural.
9. the method according to any one of claims 1 to 8 using macroporous absorbent resin separation water-soluble monascus pigment is separating
Application in water-soluble monascus pigment.
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| CN107189490B (en) * | 2017-07-07 | 2019-06-14 | 福建正味生物科技有限公司 | A kind of preparation method of high water solubility color value monascorubin |
| CN108047345A (en) * | 2017-12-21 | 2018-05-18 | 嘉兴学院 | The method of enriching and purifying black nightshade polysaccharide and the black nightshade polysaccharide obtained using this method |
| CN111748585A (en) * | 2019-03-26 | 2020-10-09 | 长江大学 | Method for increasing yield of liquid fermentation monascus pigment |
| CN110437644B (en) * | 2019-08-19 | 2020-08-07 | 长江大学 | Separation and purification method of monascus orange pigment |
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| CN105734091A (en) * | 2016-04-19 | 2016-07-06 | 天津科技大学 | Method for producing high-purity monascine and ankafaflavin through monascus liquid-state fermentation |
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