CN106589476A - Biological 3D printing planting shell wire rod and formula and manufacturing process thereof - Google Patents
Biological 3D printing planting shell wire rod and formula and manufacturing process thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y80/00—Products made by additive manufacturing
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/12—Agar or agar-agar, i.e. mixture of agarose and agaropectin; Derivatives thereof
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- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
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- C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
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Abstract
The invention discloses a biological 3D printing planting shell wire rod and a formula and manufacturing process thereof and belongs to the technical field of biological 3D printing, the biological 3D printing planting shell wire rod is prepared from phenazine derivative gel, natural gel, collagen, cell nutrients, hydrophilic additives, sterilized natural silk powder, ethyl alcohol and water. The manufacturing process includes the following steps: firstly heating and dissolving the natural gel and the collagen, then adding the chitosan, dissolving the chitosan while stirring the mixture, adding the phenazine derivative gel, the hydrophilic additives, the sterilized natural silk powder, the cell nutrients and the ethyl alcohol in the mixed system, mixing the mixture evenly and fully, evaporating a solvent, and finally conducting extrusion molding to obtain the shell wire rod. The biological 3D printing planting shell wire rod has the advantages that the content is measurable, the measurement is convenient, sterilization of the system can be conducted on the premise that normal cells are not damaged, and the safety is high.
Description
Technical field
The present invention relates to biological 3D printing technique field, more particularly to a kind of biological 3D printing plantation shell wire rod and
Its formula and processing technology.
Background technology
3D printing technique also known as increases material manufacturing technology, actually a kind of new rapid shaping technique, it be it is a kind of with
Based on mathematical model file, with powder membranous metal or plastics etc. can jointing material, come structure by way of successively printing
The technology of divine force that created the universe body, principle is, by layered manufacturing, successively to increase material to generate the technology of 3D solid.
Biological 3D printing is that one kind can be biological according to the positioning assembling of increasing material manufacturing principle in the case where digital three-dimensional model drives
Material or cell factory, the method for manufacturing medical apparatus and instruments, tissue engineering bracket and organizer official rank product.Biometric print machine is profit
With biocomponents block from level to level, go to manufacture real biological tissue, 3D biometric prints machine there can be two printheads, and one puts
For up to 80,000 human body cells are put, is referred to as " biological ink "；Another printable " biochargeable paper ", so-called biochargeable paper is in fact main
The gel for wanting composition to be water, can be used as the cell seeding shell of cell growth.Certainly, when the situation that cell seeding shell has been present
Under, 3D biometric prints machine can also only containing " biological ink " printhead.
A kind of Chinese patent " biological 3D printing cell seeding shell wire rod and its formula of Publication No. CN 105238690A
And processing technology " a kind of 3D printing cell seeding shell wire rod with dissolvable, degradable is disclosed, it is conducive to the life of cell
It is long, and stablize easy to maintain, but it cannot be determined after " plasticity ", the content of " shell " in plantation organ, and due to absorbing
Nutrition, not only promotes the growth of cell, additionally aids the growth of antibacterial, there is larger potential safety hazard.
The content of the invention
For in biological 3D printing cell seeding shell wire rod in prior art cannot really content of the shell in organ is planted with
And the defect of easy breed bacteria, a kind of biological 3D printing plantation shell wire rod of present invention offer and its formula and processing technology, it
Residual quantity of the plantation shell in organ can be measured, and security performance is high.
The formula of shell wire rod is planted in a kind of biological 3D printing, comprising following material：Phenazene derivative gel, Native Gel,
Collagen protein, cytotrophy material, hydrophilic additive, sterilizing natural silk powder, ethanol, water.
The processing technology of shell wire rod is planted in the biological 3D printing, is comprised the following steps：
(1) Native Gel and collagen protein are added in heating stirrer, water, heated and stirred is then added in agitator
To dissolving, 30～60min is incubated at 95～100 DEG C, is then progressively cooled to 75～85 DEG C；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and it is incubated 5～
10min；
(3) by the phenazene derivative factor, in confined conditions heating for dissolving in organic solvent, forms 6～10mg/ of concentration
The solution of mL, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and add hydrophilic additive, go out
Bacterium natural silk powder, cytotrophy material and ethanol, stir, sealing stirring 5～10min, Ran Houzheng at 80～90 DEG C
Excess of solvent is sent, mixed gel is obtained；
(5) mixed gel is put in extruder, the extrusion molding at 85～90 DEG C.
The present invention also provides a kind of biological 3D printing plantation shell wire rod prepared by above-mentioned processing technology.
The present invention has an advantageous effect in that compared with prior art：
1st, the phenazene derivative gel in the present invention can occur fluorescence reaction with many kinds of metal ions, when organ plastic forming
Afterwards, the residual quantity of organ cells of superficial layer shell can directly be tested；
2nd, on the one hand Native Gel has the effect for softening, and can reduce the hardness of gel, improves its plasticity, on the other hand
Can also be cells with nutrient, promote the growth of cell, collagen protein to improve the viscosity and hardness of gel rubber system, be easy to modeling
Type；
3rd, ethanol, and the high temperature storage under sealing condition are added in gel, mixed gel system is sterilized, then
It is again at high temperature that ethanol volatilization is complete, it is to avoid injury of the ethanol to cell.
There is content can survey and convenient advantage is measured for the biological 3D printing plantation shell wire rod of the present invention, moreover it is possible to not hinder
System is sterilized on the premise of evil normal cell, it is safe.
Description of the drawings
Fig. 1：The fluorescent emission figure of the azophenlyene biogel factor in the present invention.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, it is right below in conjunction with drawings and Examples
The present invention is further elaborated.It should be appreciated that specific embodiment described herein is only to explain the present invention, and
It is not used in the restriction present invention.
The invention provides the formula of shell wire rod is planted in a kind of biological 3D printing, comprising following material：Phenazene derivative coagulates
Glue, Native Gel, collagen protein, cytotrophy material, hydrophilic additive, sterilizing natural silk powder, ethanol, wherein water, azophenlyene
Derivative gel can occur fluorescence reaction with many kinds of metal ions, after organ plastic forming, can directly test organ top layer thin
The residual quantity of born of the same parents' shell；On the one hand Native Gel has the effect for softening, and can reduce the hardness of gel, improves its plasticity, another
Aspect can also be cells with nutrient, promote the growth of cell, collagen protein to improve the viscosity and hardness of gel rubber system, be easy to
Plastotype；Ethanol, and the high temperature storage under sealing condition are added in gel, mixed gel system is sterilized, then existed again
It is under high temperature that ethanol volatilization is complete, it is to avoid injury of the ethanol to cell.
According to the present invention, in order that the performance of gel be optimal it is different, further, phenazene derivative gelator 5～
30 parts, 30～80 parts of Native Gel, 5～18 parts of collagen protein, 12～20 parts of cytotrophy material, 8～15 parts of hydrophilic additive,
3～15 parts of sterilizing natural silk powder, 10～15 parts of ethanol, 30～50 parts of water, the content of each material can be aforementioned in the present invention
Arbitrary value in numerical value, it is furthermore preferred that 10～18 parts of phenazene derivative gelator, 42～60 parts of Native Gel, collagen protein 8
～10 parts, 15～20 parts of cytotrophy material, 10～15 parts of hydrophilic additive, 8～12 parts of sterilizing natural silk powder, ethanol 10～
15 parts, 30～40 parts of water.
According to the present invention, in order to improve the susceptiveness of the detection of gel, the further phenazene derivative gel
The structural formula of the factor is to be designated as (G5)
Wherein, the metal ion of fluorescence reaction can be produced to the phenazene derivative gel:Mg2+、Ca2+、Cr3+、Fe3+、
Co2+、Ni2+、Cu2+、Zn2+、Ag+、Cd2+、Hg2+、Pb2+、Ba2+、Sr2+、Al3+, therefore can be by detection gel and metal ion
Reacted fluorescence is strong and weak, tests its content, accurately content of the record cell shell in organ.
According to the present invention, the Native Gel is the Native Gel well known to the art personnel, including but do not limit
One or more in pectin, gelatin, chitosan gel rubber, agar, sodium alginate gel.
According to the present invention, in order to improve the hydrophilic of gel, hydrophilic additive is added in gel, the further parent
Water additive includes but is not limited to one or more in monosaccharide, polysaccharide, polyhydric alcohol, polysaccharide alcohol, polyethers, polyamide, preferably
Glucose, xylitol, Sorbitol, mannitol, cyclodextrin, cellulose, hemicellulose, shitosan, dextran, carapace
The copolymer of element, amylose, amylopectin, Polyethylene Glycol, polypropylene glycol, polypropylene glycol and Polyethylene Glycol, polyglycereol, poly- third
Olefin(e) acid, copolymer-maleic anhydride, hydroxymethyl cellulose, methylcellulose, hydroxymethyl-propyl cellulose, PSS,
One or more compositions in hexenoic acid etc..
According to the present invention, in order to improve cell shell and the intercellular compatibility, cytotrophy material is added in gel, entered
The cytotrophy material of one step is included but is not limited in 0.25wt% glucoses, 0.9wt% sodium chloride solutions, lump sugar in small crystal
One or more.
The present invention also provides the processing technology that shell wire rod is planted in a kind of biological 3D printing, comprises the following steps：
(1) Native Gel and collagen protein are added in heating stirrer, water, heated and stirred is then added in agitator
To dissolving, 30～60min is incubated at 95～100 DEG C, is then progressively cooled to 75～85 DEG C, by the way that slowly progressively cooling is avoided
Solution solidifies；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and it is incubated 5～
10min, promotes the dissolving of shitosan；
(3) by the phenazene derivative factor, in confined conditions heating for dissolving in organic solvent, forms 6～10mg/ of concentration
The solution of mL, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and add hydrophilic additive, go out
Bacterium natural silk powder, cytotrophy material and ethanol, stir, sealing stirring 5～10min, Ran Houzheng at 80～90 DEG C
Excess of solvent is sent, mixed gel is obtained；
(5) mixed gel is put in extruder, extrusion molding.
According to the present invention, in order to improve the solvent degree of the azophenlyene biogel factor, promote its gelation reaction, further
Organic solvent in the step (3) is one or more combination in ethanol, isopropanol, acetone, normal propyl alcohol, normal hexane, DMF
Thing, for the ease of the volatilization of the solvent in subsequent step, further, the organic solvent is preferably ethanol, isopropanol, acetone
In one or two compositionss.
According to the present invention, the plasticity to make gel is optimal state, and further, mixing is solidifying in the step (4)
The total content of water and organic solvent is 0.05～0.28% in glue.
According to the present invention, according to the physical property of gel, further, the temperature of extrusion molding is in the step (5)
75～90 DEG C, extruded velocity is 20～40rpm.
The present invention also provides a kind of making work that shell wire rod is planted by the arbitrary described biological 3D printing of claim 8～11
Biological 3D printing plantation shell wire rod prepared by skill.
Embodiment 1：
The formula of shell wire rod, the material comprising following weight portion are planted in a kind of biological 3D printing：Phenazene derivative gel because
12 parts of son, 20 parts of pectin, 30 parts of chitosan gel rubber, 10 parts of collagen protein, 18 parts of 0.9wt% sodium chloride solutions, amylose 8
Part, 5 parts of PSS, 10 parts of sterilizing natural silk powder, 12 parts of ethanol, 40 parts of water.
The processing technology of shell wire rod is planted in the biological 3D printing, is comprised the following steps：
(1) pectin, chitosan gel rubber and collagen protein are added in heating stirrer, then add water in agitator,
Heated and stirred is incubated 30～60min to dissolving at 95～100 DEG C, is then progressively cooled to 75～85 DEG C；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and it is incubated 5～
10min；
(3) by the phenazene derivative factor, in confined conditions heating for dissolving in ethanol, forms 6～10mg/mL's of concentration
Solution, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and amylose, polyphenyl are added
Vinyl sulfonic acid ester, sterilizing natural silk powder, 0.9wt% sodium chloride solutions and ethanol, stir, and seal at 80～90 DEG C and stir
5～10min is mixed, excess of solvent is then evaporated, mixed gel is obtained, the content of liquid is 0.12% in mixed gel；
(5) mixed gel is put in extruder, the extrusion molding at 80～85 DEG C, extruded velocity is 25～30rpm.
Embodiment 2：The formula of shell wire rod, the material comprising following weight portion are planted in a kind of biological 3D printing：Azophenlyene derives
10 parts of thing gelator, 20 parts of sodium alginate gel, 40 parts of gelatin, 8 parts of collagen protein, 15 parts of lump sugar in small crystal, 10 parts of mannitol,
8 parts of sterilizing natural silk powder, 15 parts of ethanol, 40 parts of water.
The processing technology of shell wire rod is planted in the biological 3D printing, is comprised the following steps：
(1) 20 parts of sodium alginate gel, gelatin and collagen protein are added in heating stirrer, is then added in agitator
Enter water, heated and stirred is incubated 30～60min at 95～100 DEG C, is then progressively cooled to 75～85 DEG C to dissolving；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and it is incubated 5～
10min；
(3) by the phenazene derivative factor in confined conditions heating for dissolving in the mixed solution of isopropanol and ethanol,
The solution of 6～10mg/mL of concentration is formed, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and mannitol, sterilizing are added
Natural silk powder, lump sugar in small crystal and ethanol, stir, 5～10min of sealing stirring at 80～90 DEG C, and it is unnecessary then to evaporate
Solvent, obtains mixed gel, and the content of liquid is 0.22% in mixed gel；
(5) mixed gel is put in extruder, the extrusion molding at 80～85 DEG C, extruded velocity is 30～35rpm.
Embodiment 3：The formula of shell wire rod, the material comprising following weight portion are planted in a kind of biological 3D printing：Azophenlyene derives
18 parts of thing gelator, 42 parts of agar, 10 parts of collagen protein, 15 parts of 0.9wt% sodium chloride solutions, 5 parts of lump sugar in small crystal, chitin 15
Part, 12 parts of sterilizing natural silk powder, 10 parts of ethanol, 50 parts of water.
The processing technology of shell wire rod is planted in the biological 3D printing, is comprised the following steps：
(1) agar and collagen protein are added in heating stirrer, then adds water in agitator, heated and stirred is to molten
Solution, is incubated 30～60min at 95～100 DEG C, is then progressively cooled to 75～85 DEG C；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and it is incubated 5～
10min；
(3) by the phenazene derivative factor, in confined conditions heating for dissolving in acetone, forms 6～10mg/mL's of concentration
Solution, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and chitin, sterilizing day are added
So Silk Powder, lump sugar in small crystal and ethanol, stir, and 5～10min of sealing stirring, then evaporates unnecessary molten at 80～90 DEG C
Agent, obtains mixed gel, and the content of liquid is 0.18% in mixed gel；
(5) mixed gel is put in extruder, the extrusion molding at 80～85 DEG C, extruded velocity is 25～30rpm.
Embodiment 4：The formula of shell wire rod, the material comprising following weight portion are planted in a kind of biological 3D printing：Azophenlyene derives
5 parts of thing gelator, 50 parts of agar, 30 parts of sodium alginate gel, 18 parts of collagen protein, 12 parts of 0.25wt% glucoses, dextrorotation
8 parts of sugared acid anhydride, 15 parts of sterilizing natural silk powder, 10 parts of ethanol, 30 parts of water.
The processing technology of shell wire rod is planted in the biological 3D printing, is comprised the following steps：
(1) agar, sodium alginate gel and collagen protein are added in heating stirrer, is then added in agitator
Water, heated and stirred is incubated 30～60min to dissolving at 95～100 DEG C, is then progressively cooled to 75～85 DEG C；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and it is incubated 5～
10min；
(3) by the phenazene derivative factor, in confined conditions heating for dissolving in DMF, forms the molten of 6～10mg/mL of concentration
Liquid, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and dextran, sterilizing are added
Natural silk powder, 0.25wt% glucoses and ethanol, stir, sealing stirring 5～10min, Ran Houzheng at 80～90 DEG C
Excess of solvent is sent, mixed gel is obtained, the content of liquid is 0.28 in mixed gel；
(5) mixed gel is put in extruder, the extrusion molding at 75～80 DEG C, extruded velocity is 20～25rpm.
Embodiment 5：The formula of shell wire rod, the material comprising following weight portion are planted in a kind of biological 3D printing：Azophenlyene derives
30 parts of thing gelator, 30 parts of chitosan gel rubber, 18 parts of collagen protein, 20 parts of 0.25wt% glucoses, polypropylene glycol and poly- second
5 parts of the copolymer 1 of glycol, 3 parts of sterilizing natural silk powder, 15 parts of ethanol, 50 parts of water.
The processing technology of shell wire rod is planted in the biological 3D printing, is comprised the following steps：
(1) chitosan gel rubber and collagen protein are added in heating stirrer, then adds water, heating to stir in agitator
Mix to dissolving, 30～60min is incubated at 95～100 DEG C, be then progressively cooled to 75～85 DEG C；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and it is incubated 5～
10min；
(3) by the phenazene derivative factor, in confined conditions heating for dissolving in normal hexane, forms 6～10mg/mL of concentration
Solution, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and add polypropylene glycol and gather
The copolymer of ethylene glycol, sterilizing natural silk powder, 0.25wt% glucoses and ethanol, stir, and seal at 80～90 DEG C
5～10min of stirring, then evaporates excess of solvent, obtains mixed gel, and the content of liquid is 0.15% in mixed gel；
(5) mixed gel is put in extruder, extrusion molding extruded velocity is 35～40rpm at 85～90 DEG C.
Bacteriostatic activity test
Staphylococcus aureus (Staphyloccocus aureus) are a kind of important pathogens of the mankind, are gram sun
Property bacterium, is under the jurisdiction of staphylococcus, can cause various severe infections, and they are also experimental strain common in laboratory, therefore are borrowed
Its power to evaluate compound Antibacterial activity.
Specifically, experiment bacteria culture media used is LB culture medium, bacteria suspension is leached with aseptic cotton carrier, be uniformly applied to training
On foster base, test sample is dissolved in DMSO, and concentration is 6.0 mg/mls, takes the nothing that 5 microlitres of samples are added to a diameter of 5 millimeters
(per the microgram of piece 30) on bacterium filter paper, dry；And with added with same volume DMSO and the filter paper that dries does negative control, it is mould with chlorine
Element is used as antibacterial positive control.37 DEG C of quiescent cultures are placed in added with the plating medium of sample 24 hours, observation experiment knot
Really, there is the measurement of inhibition zone its antibacterial circle diameter.
Table 1：Histamine result of each shell wire rod to antibacterial in embodiment 1～5
Test result indicate that, the shell wire rod of the present invention has obvious fungistatic effect.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., all should be contained within protection scope of the present invention.
Claims (12)
1. the formula of shell wire rod is planted in a kind of biological 3D printing, it is characterised in that comprising following material：Phenazene derivative gel,
Native Gel, collagen protein, cytotrophy material, hydrophilic additive, sterilizing natural silk powder, ethanol, water.
2. shell wire rod is planted in a kind of biological 3D printing as claimed in claim 1, it is characterised in that the thing comprising following weight portion
Matter：5～30 parts of phenazene derivative gelator, 30～80 parts of Native Gel, 5～18 parts of collagen protein, cytotrophy material 12
～20 parts, 8～15 parts of hydrophilic additive, 3～15 parts of sterilizing natural silk powder, 10～15 parts of ethanol, 30～50 parts of water.
3. shell wire rod is planted in a kind of biological 3D printing as claimed in claim 2, it is characterised in that the thing comprising following weight portion
Matter：10～18 parts of phenazene derivative gelator, 42～60 parts of Native Gel, 8～10 parts of collagen protein, cytotrophy material 15
～20 parts, 10～15 parts of hydrophilic additive, 8～12 parts of sterilizing natural silk powder, 10～15 parts of ethanol, 30～40 parts of water.
4. shell wire rod is planted in a kind of biological 3D printing as claimed in claim 1, it is characterised in that the phenazene derivative gel
The structural formula of the factor is
5. shell wire rod is planted in a kind of biological 3D printing as claimed in claim 1, it is characterised in that the Native Gel include but
One or more be not limited in pectin, gelatin, chitosan gel rubber, agar, sodium alginate gel.
6. shell wire rod is planted in a kind of biological 3D printing as claimed in claim 1, it is characterised in that the hydrophilic additive includes
But one or more be not limited in monosaccharide, polysaccharide, polyhydric alcohol, polysaccharide alcohol, polyethers, polyamide.
7. as shell wire rod is planted in a kind of biological 3D printing as claimed in claim 1, it is characterised in that the cytotrophy material
One or more including but not limited in glucose, sodium chloride solution, lump sugar in small crystal.
8. the processing technology of shell wire rod is planted in a kind of biological 3D printing, it is characterised in that comprised the following steps：
(1) Native Gel and collagen protein are added in heating stirrer, then adds water in agitator, heated and stirred is to molten
Solution, is incubated 30～60min at 95～100 DEG C, is then progressively cooled to 75～85 DEG C；
(2) during shitosan to be added the mixed system of step (1), stir to dissolving at 75～85 DEG C, and be incubated 5～10min；
(3) by the phenazene derivative factor, in confined conditions heating for dissolving in organic solvent, forms 6～10mg/mL's of concentration
Solution, standing is cooled to room temperature, forms azophenlyene biogel；
(4) during the azophenlyene biogel of step (3) to be added the mixed system of step (2), and hydrophilic additive, sterilizing day are added
So Silk Powder, cytotrophy material and ethanol, stir, and 5～10min of sealing stirring, then evaporates at 80～90 DEG C
Excess of solvent, obtains mixed gel；
(5) mixed gel is put in extruder, extrusion molding.
9. the processing technology of shell wire rod is planted in a kind of biological 3D printing as claimed in claim 8, it is characterised in that the step
(3) organic solvent in is one or more compositions in ethanol, isopropanol, acetone, normal propyl alcohol, normal hexane, DMF, preferably
For one or two compositionss in ethanol, isopropanol, acetone.
10. the processing technology of shell wire rod is planted in a kind of biological 3D printing as claimed in claim 8, it is characterised in that the step
Suddenly the total content of water and organic solvent is 0.05～0.28% in mixed gel in (4).
The processing technology of shell wire rod is planted in a kind of 11. biological 3D printings as claimed in claim 8, it is characterised in that the step
Suddenly the temperature of extrusion molding is 75～90 DEG C in (5), and extruded velocity is 20～40rpm.
12. present invention also provide a kind of making work that shell wire rod is planted by the arbitrary described biological 3D printing of claim 8～11
Biological 3D printing plantation shell wire rod prepared by skill.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108567992A (en) * | 2018-03-21 | 2018-09-25 | 暨南大学 | A kind of 3D printing bio-ink and preparation method thereof quickly repaired for spinal injury blood vessel |
| CN113677742A (en) * | 2019-04-03 | 2021-11-19 | 泰森德洛集团公司 | Gelatin and use thereof |
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| CN105238690A (en) * | 2015-10-31 | 2016-01-13 | 林新颖 | Biological 3D cell planting shell printing wire as well as formula and manufacturing process thereof |
| CN105936636A (en) * | 2016-05-06 | 2016-09-14 | 西北师范大学 | Preparation methods and application of long chain-containing phenazine derivative gel factor and metal gel thereof |
| CN106084689A (en) * | 2016-05-31 | 2016-11-09 | 广西医科大学 | A kind of luminous PLA wire rod for 3D printing and preparation method thereof |
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| CN105238690A (en) * | 2015-10-31 | 2016-01-13 | 林新颖 | Biological 3D cell planting shell printing wire as well as formula and manufacturing process thereof |
| CN105936636A (en) * | 2016-05-06 | 2016-09-14 | 西北师范大学 | Preparation methods and application of long chain-containing phenazine derivative gel factor and metal gel thereof |
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