CN106587343A - Method for removing water total N from surface water source by means of embedding biological active filler in wide-spectrum denitrifying bacteria - Google Patents
Method for removing water total N from surface water source by means of embedding biological active filler in wide-spectrum denitrifying bacteria Download PDFInfo
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- CN106587343A CN106587343A CN201611261468.1A CN201611261468A CN106587343A CN 106587343 A CN106587343 A CN 106587343A CN 201611261468 A CN201611261468 A CN 201611261468A CN 106587343 A CN106587343 A CN 106587343A
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- denitrifying bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2806—Anaerobic processes using solid supports for microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The invention provides a method for removing water total N from a surface water source by means of embedding biological active filler in wide-spectrum denitrifying bacteria and belongs to the field of water treatment. The adopted denitrifying bacteria are screened out and separated from nitrified sludge in a urban sewage treatment plant, and a wide-spectrum maintaining method with hydrolysate of remaining sludge in the urban sewage treatment plant as denitrifying carbon source supply is adopted; the nitrogen removal capacity of the denitrifying bacteria obtained through enrichment culture is converted into the fact that the nitrogen removal capacity of a denitrifying mixed solution needs to reach 300 mg/L.h or above under the conditions that the concentration of the denitrifying bacteria is 6000 mg/L, the temperature is 25 DEG C, and CODCr/ nitrate nitrogen is 4=7; bacterium embedding is conducted; the filler is placed in water with one end fixed to a base and the other end in a free state; and the surrounding area of a water intake opening is used as a feeding area, the C/N ratio in the filler feeding area is kept being 4 or above. By the adoption of the method for removing the water total N from the surface water source by means of embedding the biological active filler in the wide-spectrum denitrifying bacteria, the water quality is stable in a water intake area, the water intake area does not need to be replaced within several years, and the method is not influenced by temperature.
Description
Technical field
The invention belongs to water treatment field, more particularly to a kind of to embed biological active filling material pin with broad spectrum activity denitrifying bacteria
The technical method that N total to source water is removed.
Background technology
Source water N pollutions speculate it is with NH by traditional theory4 +- N is principal mode presence, investigates but more
Research Literature shows, source water (river, lake, reservoir) is acted on due to water surface hydraulic jump reoxygenation, the N of low-intensity pollution it is many with
NO3 -- N forms are present.NO in Rhodobacter Spheroides surface water3 -- N concentration change scope is 0.62-23.47mg/L, and mean concentration is
17.07mg/L, C/N=9.52；Huanghe valley Dongying Duan Lisan ditch water systems nitrate is 9-11mg/L, C/N=
5.56-7.27；The Tianjin surface water NO of Haihe River water system3 -- N inspection ranges are 0.01-16.51mg/L, partly more than 11.29mg/
L.The United Nations's health organization (WHO) specifies drinking water NO3 -The standard of-N mass concentrations is less than 10mg/L.
Conventional Water purification unit such as coagulation, precipitation, filter the nitrate nitrogen content that can not effectively reduce in raw water,
Treating nature water technology (chemistry, physics, biology etc.) then becomes the first-selection that water treatment plant removes nitrate nitrogen.Compare it is more chemical and
Physical method, because its repair time is short, environmental effect is little etc., advantage is increasingly subject to favor to biologic pretreatment method.In biological treatment
It is effect on environment minimum, nothing two for the nitrate nitrogen in source water and nitrite nitrogen denitrification biochemical reaction in technology
The best technological approaches of secondary pollution, the core antibacterial of the method is denitrifying bacterium.But, for the vast water body of surface water, adopt
Which kind of mode denitrifying bacterium is kept to keep higher concentration in water source intake region and be not lost in, always the technical method reality
The key technology obstacle applied.The fact that another is more acute be water source surface water especially in northern area, water temperature in winter is low
To 4 DEG C or so, summer water temperature high level can reach more than 20 DEG C.This temperature change is even more biologic treating technique and is difficult to fine reality
The another obstacle applied.
The content of the invention：
For the problems referred to above, by the further investigation to denitrifying bacteria, the present invention adopt broad spectrum activity denitrifying bacteria for
Bacterium source, using antibacterial embedding techniqueses the problems referred to above are relatively efficiently solved, and have createed the skill for being suitable for source water N removal
Art method.
The core of the technology is：
Using antibacterial embedding techniqueses, (portion of techniques is using invention disclosed patent before the applicant
(CN103951083B) technical method), realize the reservation of antibacterial, antibacterial is solved in big, even current water
Antibacterial losing issue；
Using broad spectrum activity denitrifying bacteria, source water is solved due to the big and caused bacterial adaptation of temperature change
Problem.Using the broad spectrum activity denitrifying bacteria of present invention culture, the different bacterium class of 20 DEG C of high temperature to 4 DEG C of low temperature ranges has been adapted to
Group, these different types of flora antibacterials, can be with the change gradually shape of temperature during water body environment temperature is gradually changed
Into the flora advantage for adapting to water temperature, so that certain denitrification biochemistry ability can be protected with the change of temperature
Hold, despite certain fluctuation but to water source surface water 30mg/L or so and following nitrate nitrogen (nitrate nitrogen and nitrous acid
Salt nitrogen) removal have stablize excellent performance.
The present invention embeds the side that biological active filling material N total to source water is removed with broad spectrum activity denitrifying bacteria
Method, it is characterised in that comprise the following steps：
(1) enrichment culture of broad spectrum activity denitrifying bacteria：In embedding denitrifying bacteria bacterium source incubation, first have to protect
Hold the broad spectrum activity of denitrifying bacteria.The denitrifying bacteria that the present invention is adopted is the screening point from municipal sewage plant's nitrifying sludge
Separate out what is come, broad spectrum activity keeping method is supplied using sewage treatment plant residual sludge hydrolytic liquid as denitrifying carbon source, to keep
Broad spectrum activity flora characteristic in culture sludge；So that the denitrification ability of the denitrifying bacteria of enrichment culture：Convert for denitrification it is thin
Bacteria concentration 6000mg/L concentration, 25 DEG C, CODCrUnder the conditions of/nitrate nitrogen=4-7 (preferably 6), denitrification mixed liquor denitrification ability
Reach more than 300mg/L.h；
(2) antibacterial embedding is carried out to the broad spectrum activity denitrifying bacteria of step (1) enrichment culture, antibacterial embedding adopts boric acid two
Secondary crosslinking technological, wherein filler adopt composite construction：With polyethylene, polrvinyl chloride, polypropylene, ABS resin Polymer material
Based on, add 6wt%-9wt% polyvinyl alcohol (PVA) water wetted material, by strand hot melt or sheet material hot pressing pore-forming mode system
Into the space network carrier of lengthy motion picture shape；A length of 0.1-3m of the lengthy motion picture shape, a width of 15-20mm；Net in network structure
Kong Kewei circles, rhombus, square, rectangle；Cancellated string diameter is 0.4-2mm；According to polyvinyl alcohol-boric acid secondary cross-linking
Method, the concentrated solution of the broad spectrum activity denitrifying bacteria of step (1) enrichment culture and poly-vinyl alcohol solution are mixed, and add carbonic acid
Calcium is prepared into embedding liquid, and polyvinyl alcohol mass concentration is 80-100g/L in embedding liquid, and Calcium Carbonate mass concentration is 10-40g/L,
The weight/mass percentage composition of broad spectrum activity denitrifying bacteria is not less than 5%；Above-mentioned embedding liquid is uniformly coated on mesh carrier, is put into
In saturation boric acid solution after 1-3h, boric acid solution pH to 8-10 is adjusted, be crosslinked 3-24h；It is drawn off, cleans surface residue
Matter, obtains broad spectrum activity denitrifying bacteria immobilized biological activated filler of the strip based on mesh carrier.
The embedding amount of broad spectrum activity denitrifying bacteria will reach embedding liquid mass percent more than 5%.The part core is
The embedding basic crack of various denitrifying bacteriums is kept, is also to realize stability and durothermic basis.
(3) dosing method：Pedestal is added as fixation by the use of heavy water solid material, by strip based on the wide of mesh carrier
The immobilized biological activated filler one end of spectrality denitrifying bacteria is fixed on pedestal, and the other end is in upwards free state, makes whole
In fixed floating state in water, pack length can determine bar filler according to the fixed location depth of water, keep upper free ends
Flooding below the water surface just can be with；The broad spectrum activity denitrifying bacteria immobilization biological of pedestal and strip based on mesh carrier is lived
Property filler is put into together in source water；
(4) position and density are added：, as region is added, regional extent will be added around source water intake region
More than the corresponding regional extent of water withdrawal on the two, broad spectrum activity denitrifying bacteria immobilization biological of the strip based on mesh carrier is lived
Property filler dosage be to add (broad spectrum activity denitrification of the every strip based on mesh carrier that the 5 ‰ of region water body volume be advisable
The volume of bacteria adhension biological active filling material is calculated with the length and width of lengthy motion picture shape and cancellated product).
(5) C/N ratios：Denitrification biological respinse needs Organic substance as electron acceptor, for the denitrification effect realized, fills out
Material adds area will keep C/N to be advisable than more than 4.
Solve that earth surface water source water volume is big using technical scheme, flowing water it is unstable caused by denitrifying bacteria not
Can stably fix and the uncontrollable problem for adding, while also solving source water because season difference causes the temperature difference to cause greatly
Denitrifying bacteria can not be well adapted for and cause the low problem of biodegradability.Using technical scheme so that
The water stabilization in source water intake region, being not required to change in several years can also obtain effluent quality well, also not by temperature
The impact of degree.
Description of the drawings：
Fig. 1 fillers fix schematic diagram
(1) filler, (2) fixed pedestal；
Fig. 2 fillers add area schematic
(3) intake, (4) are fixed on pedestal filler, (5) water intaking bank, (6) add region.
Specific embodiment
With reference to embodiment, the invention will be further described, but the present invention is not limited to following examples.
Embodiment 1：
(1) denitrifying bacteria enrichment, using municipal sewage plant's nitrifying sludge, excess sludge hydrolyzed solution as carbon source,
Screening and enriching denitrifying bacteria obtain broad spectrum activity denitrifying bacteria bacterium source；Laboratory is tested：Denitrifying bacteria concentration exists
6000mg/L, 25 DEG C, CODCrUnder the conditions of/nitrate nitrogen ≈ 6, denitrification denitrogenation efficiency can reach 300mg/ (Lh).
(2) filler makes：Filler adopts composite construction：With macromolecules such as polyethylene, polrvinyl chloride, polypropylene, ABS resin
Based on material, add 8wt% polyvinyl alcohol (PVA) water wetted material, made by strand hot melt or sheet material hot pressing pore-forming mode
The space network carrier of lengthy motion picture shape, by the concentrated solution and polyethylene of the broad spectrum activity denitrifying bacteria of step (1) enrichment culture
Alcoholic solution mixes, and adds Calcium Carbonate and be prepared into embedding liquid, and polyvinyl alcohol mass concentration is 80g/L in embedding liquid, and carbonic acid is calcareous
Amount concentration is 20g/L, and the weight/mass percentage composition of broad spectrum activity denitrifying bacteria is not less than 6%；Above-mentioned embedding liquid is uniformly coated on
On mesh carrier, it is put in saturation boric acid solution after 1-3h, adjusts boric acid solution pH to 8-10, is crosslinked 3-24h；It is drawn off,
Clean remained on surface material, obtains broad spectrum activity denitrifying bacteria immobilized biological activated filler of the strip based on mesh carrier.
Pack length 3m, width 15mm, thickness (string diameter) 2.5mm.
(4) surface water raw water test：
Filler one end is fixed on pedestal, and the other end is in upwards free state, by source water intake region week
Enclose as region is added, add regional extent for the corresponding regional extent of water withdrawal on the two, filler dosage is to add regional water
The 5 ‰ of body volume；Raw water nitrate nitrogen 30mg/L, CODCr/ nitrate nitrogen ≈ 4, ambient temperature be respectively 4 ± 1 DEG C, 15 ± 2 DEG C, 29
Under conditions of ± 1 DEG C, nitric efficiency is respectively：4.8mg/ (Lh), 4.3mg/ (Lh), 8.7mg/ (Lh), the denitrogenation effect
Rate can realize the stable denitrogenation processing of source water under source water N pollution intensities.The research for comparing foreign countries has substantially
Advantage (Robertson WD et al., at T=7.7 DEG C, nitrate nitrogen removal rate be 0.142mg/ (Lh)；Van Driel
PW et al., at T=13 DEG C, nitrate nitrogen removal rate is 0.154mg/ (Lh)；Robertson WD et al., at T=10 DEG C
When, nitrate nitrogen removal rate is 0.417mg/ (Lh)).
Claims (3)
1. the method that biological active filling material N total to source water is removed, its feature are embedded with broad spectrum activity denitrifying bacteria
It is to comprise the following steps：
(1) enrichment culture of broad spectrum activity denitrifying bacteria：In embedding denitrifying bacteria bacterium source incubation, first have to keep anti-
The broad spectrum activity of nitrobacteria；Using denitrifying bacteria be from screening and separating in municipal sewage plant's nitrifying sludge out,
Broad spectrum activity keeping method using sewage treatment plant residual sludge hydrolytic liquid as denitrifying carbon source supply, to keep culture sludge in
Broad spectrum activity flora characteristic；So that the denitrification ability of the denitrifying bacteria of enrichment culture：Convert and exist for denitrifying bacteria concentration
6000mg/L, 25 DEG C, CODCrUnder the conditions of/nitrate nitrogen=4-7, denitrification mixed liquor denitrification ability will reach more than 300mg/L.h；
(2) antibacterial embedding is carried out to the broad spectrum activity denitrifying bacteria of step (1) enrichment culture, antibacterial embedding adopts the secondary friendship of boric acid
Connection technology, wherein filler adopt composite construction：Based on polyethylene, polrvinyl chloride, polypropylene or ABS resin macromolecular material,
Addition 6wt%-9wt% polyvinyl alcohol (PVA) water wetted material, by strand hot melt or sheet material hot pressing pore-forming mode lengthy motion picture is made
The space network carrier of shape；According to polyvinyl alcohol-boric acid secondary cross-linking method, by the broad spectrum activity of step (1) enrichment culture
The concentrated solution of denitrifying bacteria and poly-vinyl alcohol solution mix, and add Calcium Carbonate and be prepared into embedding liquid, polyethylene in embedding liquid
Alcohol mass concentration be 80-100g/L, Calcium Carbonate mass concentration be 10-40g/L, the weight/mass percentage composition of broad spectrum activity denitrifying bacteria
It is not less than 5%；Above-mentioned embedding liquid is uniformly coated on mesh carrier, is put in saturation boric acid solution after 1-3h, adjust boric acid
PH value of solution is crosslinked 3-24h to 8-10；It is drawn off, cleans remained on surface material, obtains wide spectrum of the strip based on mesh carrier
The property immobilized biological activated filler of denitrifying bacteria；
(3) dosing method：Pedestal is added as fixation by the use of heavy water solid material, by broad spectrum activity of the strip based on mesh carrier
The immobilized biological activated filler one end of denitrifying bacteria is fixed on pedestal, and the other end is in upwards free state, fills out whole piece
In fixed floating state in water, pack length determines material according to the fixed location depth of water, keep upper free ends the water surface with
Under flood；Pedestal and strip are put into together based on the immobilized biological activated filler of broad spectrum activity denitrifying bacteria of mesh carrier
To in source water；
(4) position and density are added：, as region is added, regional extent will be added and be more than around source water intake region
The corresponding regional extent of water withdrawal on the two, broad spectrum activity denitrifying bacteria of the strip based on mesh carrier is immobilized biological activated to fill out
Material dosage is add region water body volume 5 ‰；
(5) C/N ratios：Denitrification biological respinse needs Organic substance as electron acceptor, and filler adds area and to keep C/N than more than 4.
2. entered with broad spectrum activity denitrifying bacteria embedding biological active filling material N total to source water according to described in claim 1
The method that row is removed, it is characterised in that CODCr/ nitrate nitrogen=6.
3. entered with broad spectrum activity denitrifying bacteria embedding biological active filling material N total to source water according to described in claim 1
The method that row is removed, it is characterised in that a length of 0.1-3m of step (2) lengthy motion picture shape, a width of 15-20mm；Net in network structure
Hole is circle, rhombus, square, rectangle；Cancellated string diameter is 0.4-2mm.
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| CN201611261468.1A CN106587343B (en) | 2016-12-30 | 2016-12-30 | method for removing total N in surface water source water by embedding bioactive filler with broad-spectrum denitrifying bacteria |
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| CN201611261468.1A CN106587343B (en) | 2016-12-30 | 2016-12-30 | method for removing total N in surface water source water by embedding bioactive filler with broad-spectrum denitrifying bacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109455824A (en) * | 2018-12-17 | 2019-03-12 | 上海同瑞环保科技有限公司 | A kind of preparation method of immobilized microorganism filler |
| CN111484123A (en) * | 2020-05-06 | 2020-08-04 | 北京工业大学 | Efficient nitrifying embedded bioactive filler and preparation method thereof |
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| CN103896407A (en) * | 2013-12-19 | 2014-07-02 | 北京京阳环保工程有限公司 | Quick start biofilm culturing carbon microorganism combined water purification method |
| CN103951031A (en) * | 2014-04-04 | 2014-07-30 | 北京工业大学 | Preparation and application of denitrifying bacteria immobilized fixed-type bioactive fillers based on reticulate carriers |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103896407A (en) * | 2013-12-19 | 2014-07-02 | 北京京阳环保工程有限公司 | Quick start biofilm culturing carbon microorganism combined water purification method |
| CN103951031A (en) * | 2014-04-04 | 2014-07-30 | 北京工业大学 | Preparation and application of denitrifying bacteria immobilized fixed-type bioactive fillers based on reticulate carriers |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109455824A (en) * | 2018-12-17 | 2019-03-12 | 上海同瑞环保科技有限公司 | A kind of preparation method of immobilized microorganism filler |
| CN111484123A (en) * | 2020-05-06 | 2020-08-04 | 北京工业大学 | Efficient nitrifying embedded bioactive filler and preparation method thereof |
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| CN106587343B (en) | 2019-12-13 |
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