CN106576481A - Method for screening sorghum variety suitable for cadmium polluted soil repair, and new application of sorghum variety - Google Patents
Method for screening sorghum variety suitable for cadmium polluted soil repair, and new application of sorghum variety Download PDFInfo
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- CN106576481A CN106576481A CN201611117569.1A CN201611117569A CN106576481A CN 106576481 A CN106576481 A CN 106576481A CN 201611117569 A CN201611117569 A CN 201611117569A CN 106576481 A CN106576481 A CN 106576481A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention relates to a method for screening sorghum variety suitable for cadmium polluted soil repair as well as identification of the available variety. The method comprises the steps: germinating sorghum seeds, cultivating seedlings in a cadmium-containing nutritional solution, harvesting roots and overground parts and drying until constant weight; according to the dry weight of the roots and the overground parts, calculating the corresponding subordinative function value by a fuzzy mathematic subordinate function method, calculating the tolerance coefficient of the sorghum strains, performing cadmium tolerance evaluation and dividing the sorghum strains into multiple grades; taking the dry weight of the roots as the reference index on the cadmium absorption quantity of the sorghum strains, screening the strain with high cadmium absorption quantity from the sorghum strains with relatively high tolerance so as to screening the sorghum variety suitable for cadmium polluted soil repair. The invention provides a method for quickly searching a sorghum variety with high cadmium absorption quantity and a spare sorghum variety resource applicable to soil repair, and provides beneficial reference for utilization of the energy plant sorghum on cadmium polluted soil repair.
Description
Technical field
The invention belongs to technical field of soil remediation, and in particular to a kind of Sorghum Germplasm repaired suitable for cadmium pollution soil
The new application of screening technique and Sorghum Germplasm.
Background technology
With mining, the development of metallurgical industry, phosphate fertilizer and insecticide are excessively used, and cadmium pollution becomes increasingly severe
(Zhang et al.,2015).Cadmium has a strong impact on the growth promoter of plant as one of the heavy metal of most toxicity, causes to make
The thing underproduction, quality decline.More seriously, cadmium can be accumulated by food chain in human body, cause pulmonary carcinoma and skeletal abnormality etc. many
Kind disease (Bertin＆Averbeck, 2006；Dias et al.,2013).Therefore, administer cadmium pollution and seem particularly urgent.Plant
Thing reparation is a kind of eco-friendly, economic recovery technique, is gradually paid close attention to by people in recent years.But currently used for plant
That what is repaired mostly is super enriching plant, and not only Biomass is little, poor growth, and economic benefit is low, it is difficult to extensive in practice
Using (Liu et al., 2011).Therefore it has been proposed that can with the general plant of high-biomass especially energy-source plant (such as Ramulus Salicis Babylonicae
Millet, Sorghum vulgare Pers. and Miscanthus etc.) repairing heavy metal pollution soil (Chen et al., 2011；Metwali et al.,2013；
Zhang et al.,2014).Although the amount of these plant absorption heavy metals is less, because of Biomass height, the huge sum of money for finally absorbing
Category total amount may be more.Yang Yongs etc. (2009) compare super enriching plant Thalaspi arvense L. and 3 kinds of high-biomass plant Indian mustards
(Brassica juncea), Nicotiana tabacum L. (Nicotiana tabacum L.) and Helianthi (Helianthus annuus L.) are carried
The difference of Cd efficiency is taken, as a result shows that Nicotiana tabacum L. is most to the extracted amount of Cd, be 4 times of super enriching plant Thalaspi arvense L. to Cd extracted amounts
Left and right, is 13 times of Helianthi to Cd extracted amounts, and so as to demonstrate some high-biomass plants heavy metal polluted soil is can be used for
The reparation of earth.
Sorghum vulgare Pers. is a kind of C4Plant, with very high photosynthetic efficiency, is classified as by USDOE and can be used to meet latent
One of important draft energy-source plant in biomass Production requirement (Gill et al., 2014；Gnansounou et al.,
2005).The seed of Sorghum vulgare Pers., sugar (mainly sweet height in rich cellulose, the stem of hemicellulose and leaf, and aerial partss stalk
Fine strain of millet) it is used equally to alcohol production.There are some researches show that Sorghum vulgare Pers. has good absorbability to lead, cadmium, nickel, chromium, zinc and copper etc.
(Al Chami et al.,2015；Bonfranceschi et al.,2009；Salman et al., 2013), and with it is little
Wheat is compared with Semen Maydiss, Sorghum vulgare Pers. cadmium and copper are had higher toleration (Metwali et al., 2013).In cadmium pollution soil plantation
Sorghum vulgare Pers. (sugar grass) can not only provide raw material for alcohol production, and can reach the purpose of soil remediation.Additionally, alcohol production
The cellulose residue for producing afterwards, can be used for combustion power generation, further can from the ashes reclaim heavy metal (Woods 2001；
Li 2013).Through this process, cadmium is from food chain transport to Energy Chain, it is to avoid harm to human body.
The different cultivars or strain of same plant has very big difference in terms of cadmium tolerance and absorption.Barzanti etc. (2011)
The different sweetalyssums (Alyssum) of report have notable difference in terms of the toleration of cadmium and accumulation.Yang etc. (2015) compares 24
Performance of individual willow (Salix spp.) strain under manganese process, as a result shows different lines in terms of the toleration of manganese and accumulation
There is very big difference.Arsenic in the seed and aerial partss stalk of the lower 12 wheat cultivation kinds of Shi etc. (2015) research discovery same treatments
With cadmium concentration significant difference.Therefore, the high kind of heavy metal absorbtivity or strain are found to planting by the method for germplasm screening
Thing reparation puts into practice significant.
It is fewer with regard to the research of different Sorghum Germplasm heavy metal absorbability differences both at home and abroad, and be with phytoremediation
The Sorghum Germplasm screening of purpose is even more and is rarely reported.Soudek etc. (2013) is by 6 Sorghum vulgare Pers. (sweet height under the conditions of measure water planting
Fine strain of millet) root and aerial partss cadmium content, compare their differences in terms of Cd uptake ability.Nationality etc. (2014) is tried by field
Test, determine hydrargyrum (Hg), cadmium (Cd), manganese (Mn), zinc (Zn), cobalt (Co), chromium (Cr), lead in 11 Sorghum vulgare Pers. (sugar grass) kinds
(Pb) and 8 heavy metal species such as copper (Cu) content, to evaluate their differences to different Metal uptake abilities.Existing technology
Generally require and evaluate absorbability of the strain to cadmium by determining the cadmium content of Sorghum vulgare Pers., although results contrast reliability, but it is right
In large number of germ plasm resource, the method seems time-consuming, effort, high cost.And if directly carrying out field test, there is soil again
The problems such as earth condition is difficult to control to.
The content of the invention
It is an object of the invention to provide a kind of Sorghum Germplasm screening technique repaired suitable for cadmium pollution soil.
The Sorghum Germplasm screening technique repaired suitable for cadmium pollution soil provided by the present invention, comprises the steps：
1) sorghum seeds are sprouted, the seedling for obtaining are cultivated in the nutritional solution containing cadmium, point root and aerial partss are harvested,
The root and aerial partss of the sorghum seedling collected are dried to constant weight respectively, is then weighed；
2) root dry weight is calculated using fuzzy mathematics Subordinate Function and is subordinate to according to the root and the dry weight of aerial partss of Sorghum vulgare Pers. respectively
Membership fuction value and aerial partss dry weight membership function value, further according to the root dry weight membership function value and overground part of a certain Sorghum vulgare Pers. strain
Dry weight membership function value is divided to calculate the tolerance coefficient of the Sorghum vulgare Pers. strainSo as to carry out the cadmium toleration of the strain Sorghum vulgare Pers.
Evaluate, and Sorghum vulgare Pers. strain is divided into into multiple grades；
3) correlation analysiss and path analysiss are carried out, determines the important references that root dry weight is Sorghum vulgare Pers. strain Cd uptake amount size
Index, cadmium is filtered out according to the root dry weight of each strain from the relatively strong Sorghum vulgare Pers. strain of toleration (I and II grade strains)
The high strain of absorbtivity, that is, filter out the Sorghum Germplasm repaired suitable for cadmium pollution soil.
Said method step 1) in, the nutritional solution concretely 1/2Hoagland nutritional solutions.
The composition of the 1/2Hoagland is：2.5mM Ca(NO3)2,2.5mM KNO3,0.5mM KH2PO4,1mM
MgSO4, 0.01mM Fe-EDTA and trace element.
Cd ultimate densities are 5-15 μM in the nutritional solution.
The culture is hot-house culture.
The condition of hot-house culture is：25 DEG C of daytime, 20 DEG C of evening, illumination 16h, relative humidity 60%.
The drying can be carried out in 70 DEG C of baking oven.
Said method step 2) in, the tolerance coefficient of a certain Sorghum vulgare Pers. strainFor its root dry weight membership function value and
The meansigma methodss of aerial partss dry weight membership function value.
Said method step 2) in, Sorghum vulgare Pers. strain is divided into by five grades according to tolerance coefficient magnitude, I grades are that cadmium is resistance to
By the most strong strain of propertyIi grade is the stronger strain of cadmium tolerationIi I etc.
Level is the medium strain of cadmium tolerationIv grade is the sensitive strain of cadmium The
V grades are most sensitive strain
Application of the Sorghum Germplasm filtered out by said method in cadmium pollution soil reparation falls within the guarantor of the present invention
Shield scope.
The Sorghum Germplasm is CHEDOMBA, and Dhurra No.7, MN 4138, Juar (are numbered " 52 "), MALNAL,
NYTWAL, UMM EL TEIMAN, Co.1, MN 2089 or ETS 3080.
Preferably, the Sorghum Germplasm is CHEDOMBA, Dhurra No.7 or MN 4138.
The present invention root dry weight, cadmium content and ground in aerial partss dry weight, root after to 96 Sorghum vulgare Pers. strain Cadmium treateds
Part cadmium content carries out correlation analysiss and path analysiss with individual plant cadmium total amount, as a result shows that root dry weight is straight with individual plant cadmium total amount
Connect interaction most strong, therefore root dry weight can more reflect extractability of the Sorghum vulgare Pers. to cadmium, can be as prediction Sorghum vulgare Pers. to Cd uptake
The emphasis reference index of amount.In actual applications, the different Sorghum vulgare Pers. strains to growing under the same terms can be big by root dry weight
It is little strong and weak to judge its absorption cadmium ability.Workload is thus considerably reduced, cost is reduced, the time is saved.
The invention provides a kind of method of the high Sorghum Germplasm of fast searching Cd uptake amount, is using energy-source plant Sorghum vulgare Pers.
(sugar grass) cadmium pollution soil repair provides beneficial reference.
The innovation of the inventive method is：
A. the evaluation methodology of energy-source plant Sorghum vulgare Pers. cadmium toleration is established；
B. according to the dry weight of 96 Sorghum vulgare Pers. strain basic data proposition roots as preliminary assessment Sorghum vulgare Pers. absorbability weight
Want reference index；
C. the standard that screening is adapted to the Sorghum vulgare Pers. strain of phytoremediation is established.
Description of the drawings
Fig. 1 is the tolerance coefficient level scattergram of different Sorghum vulgare Pers. strains in embodiment.
Fig. 2 roots and aerial partss each dry weight and cadmium concentration and individual plant cadmium total amount correlation analysiss.
Fig. 3 is the root dry weight scattergram of different Sorghum vulgare Pers. strains.
Fig. 4 is different Sorghum vulgare Pers. strain individual plant cadmium total amounts.
Specific embodiment
Below by specific embodiment, the present invention will be described, but the invention is not limited in this.
Experimental technique used in following embodiments if no special instructions, is conventional method；Institute in following embodiments
Reagent, material etc., if no special instructions, commercially obtain.
Embodiment
1st, test material
96 parts of sorghum seeds, come from germ plasm resource depositary institution of United States Department of Agriculture (USDA) (Plant Genetic Resources
Conservation Unit of the United States Department of Agriculture).Specifying information is shown in
Table 1.
Table 1 test Sorghum vulgare Pers. material number, United States Department of Agriculture's germ plasm resource accession number and title
2nd, EXPERIMENTAL DESIGN
Seed is soaked in deionized water 12h under room temperature, is then placed on to be covered with the pallet of a metafiltration paper and is sprouted, 3 days
Select afterwards normally to sprout and grow consistent seedling and move on on 96 orifice plates (cutting off bottom), cultivate one week in tap water, then
Tap water is changed to into 1/2Hoagland nutritional solutions, treatment group adds corresponding CdCl2Cd ultimate densities are made to be 10 μM, matched group
Then it is not added with.In hot-house culture 2 weeks, one time of nutrition liquid was changed in centre, divided root and aerial partss to harvest afterwards.3 repetitions are often processed, often
Repeat 5 plants.Hot-house culture condition：25 DEG C of daytime, 20 DEG C of evening, illumination 16h, relative humidity 60%.The composition of 1/2Hoagland
For 2.5mM Ca (NO3)2,2.5mM KNO3,0.5mM KH2PO4,1mM MgSO4, 0.01mM Fe-EDTA and trace element.
3rd, index determining and calculating
Dry weight is determined：Then the root and aerial partss of the sorghum seedling collected are weighed in 70 DEG C of oven for drying to constant weight；
Cadmium content is determined：The root and aerial partss of the Sorghum vulgare Pers. after drying are ground into powder, 0.2g or so is taken, are added
6ml nitric acid overnight, is subsequently adding 2ml hydrogen peroxide, is stored at room temperature 20min, with microwave digestion system (MARS；CEM
Corporation, Matthews, NC, USA) cleared up.Inductive coupling plasma emission spectrograph is used after clearing up
(6300) Thermo Electron Corporation determine cadmium content；
Tolerance coefficient is calculated：The cadmium toleration of Sorghum vulgare Pers. is evaluated using fuzzy mathematics Subordinate Function.Membership function
Value X (μ)=(X-Xmin)/(Xmax-Xmin), in formula X be index determining value, Xmin、XmaxFor a certain index of all materials of participating in the experiment most
Little value and maximum.Respectively its corresponding membership function value, a certain Sorghum vulgare Pers. strain are calculated according to Radix Sorghum vulgare Pers. and aerial partss dry weight
Tolerance coefficientAs meansigma methodss of its root dry weight membership function value and aerial partss dry weight membership function value.According to resistance to
Sorghum vulgare Pers. strain is divided into into five grades by coefficient magnitude, I grades are the most strong strain of cadmium tolerationIi
Grade is the stronger strain of cadmium tolerationIi I grade is the medium strain of cadmium tolerationIv grade is the sensitive strain of cadmiumV grades are most sensitive strain(Liu 2005；Ci et al.,2011).
4th, evaluate and screening technique
Toleration evaluation of 4.1 Sorghum vulgare Pers. to cadmium
In order to evaluate toleration of the Sorghum vulgare Pers. to cadmium, we introduce the tolerance coefficient of cadmium.Calculate different index membership functions
Value, and its meansigma methods is taken as tolerance coefficient, to show that different Sorghum vulgare Pers. strains tolerate the power of Cd stress ability.According to tolerance system
Number size, is divided into 5 grades, wherein I and II grades belong to the strong strain of toleration.Have 1 and 5 respectively in 96 Sorghum vulgare Pers. strains
Individual strain belongs to the two grades, accounts for the 6.25% of all Sorghum vulgare Pers. strains.It is ' Dhurra wherein to the most strong strain of cadmium toleration
No.7 ' (numbering ' 31 '), is secondly ' CHEDOMBA ' (numbering ' 38 '), ' IS 2352 ' (numbering ' 60 '), ' Juar ' (numbering
' 51 '), ' MN 4138 ' (numbering ' 62 '), ' ETS 3080 ' (numbering ' 72 ').In addition have 18,35 corresponding with 37 strains respectively
Ii I, IV and V grades (see Fig. 1).
4.2 Sorghum vulgare Pers. absorb the merit rating of cadmium
Root and aerial partss dry weight and root and aerial partss cadmium content have together decided on absorbtivity of the Sorghum vulgare Pers. to cadmium.It is related
Property analysis show cadmium content and aerial partss cadmium content correlation coefficient in individual plant cadmium total amount and aerial partss dry weight, root dry weight, root
Respectively 0.649,0.621,0.436 and 0.263, and all there is extremely significantly correlated (see Fig. 2).Path analysiss can reflect not
With factor interaction relationship directly or indirectly, so as to clearly illustrate relative importance of these factors to a certain index
(Dewey and Lu,1959；Akanda and Mundit,1996).We are further with root and aerial partss dry weight and root
It is independent variable with aerial partss cadmium content, path analysiss has been carried out as Dependent variable, to the absorbtivity of cadmium with Sorghum vulgare Pers..As a result root is shown
Dry weight has most strong direct effect to individual plant cadmium total amount (direct path coefficient is 0.660).Although aerial partss dry weight and list
Strain cadmium total amount has extremely significant positive correlation, and correlation coefficient reaches 0.649, but wherein aerial partss dry weight is to individual plant cadmium total amount
Direct function coefficient be only 0.253, major part is the indirect action (path coefficient produced to individual plant cadmium total amount by root dry weight
0.535) (to be shown in Table 2).Therefore, compared with other factors, root dry weight can more reflect extractability of the Sorghum vulgare Pers. to cadmium.Root dry weight can
Using the important references index as prediction Sorghum vulgare Pers. to Cd uptake amount.
Path analysiss of the table 2 with regard to total cadmium content1
1Individual plant cadmium total amount is Dependent variable, and root and aerial partss dry weight and root and aerial partss cadmium content are independent variable；2Slightly
The numerical value that body shows acts on the direct path coefficient of individual plant cadmium total amount for relevant parameter
The 4.3 Sorghum Germplasm Integrated Selection methods for being used for phytoremediation
The evaluation methodology of comprehensive above Sorghum vulgare Pers. cadmium toleration and absorbtivity, it is proposed that the Sorghum vulgare Pers. of suitable phytoremediation below
Germplasm screening method.Carry out toleration evaluation (as described in 4.1) to candidate's Sorghum Germplasm first, as select tolerance coefficient high as possible
Strain so as to complete normal life cycle, and ensure economic benefit.While the effect in order to improve phytoremediation, needs
The strain for selecting Cd uptake amount high.According to 4.2, root dry weight can refer to as the important references of Sorghum vulgare Pers. strain Cd uptake amount size
Mark.We further from the high strain of the tolerance property coefficient of screening, select the high strain of root dry weight as final target product
System.
By taking this screening as an example, 6 strains of toleration strong (tolerance coefficient is high) are followed successively by ' Dhurra No.7 ' (numberings
' 31 '), ' CHEDOMBA ' (numbering ' 38 '), ' ' MN 4138 ' (is compiled for IS 2352 ' (numbering ' 60 '), ' Juar ' (numbering ' 51 ')
Numbers ' 62 '), ' ETS 3080 ' (numbering ' 72 ').Further this 6 strain root dry weights sort be followed successively by from big to small：‘Dhurra
No.7’>‘CHEDOMBA’>‘MN 4138’>‘Juar’>‘IS 2352’>' ETS 3080 ', and this 6 strains are dry in root
In high front 10 strains of weight (see Fig. 3), thus it is presumed that ' Dhurra No.7 ', ' CHEDOMBA ' and ' MN may be selected
4138 ' used as the strong target strain of Cd uptake ability.In order to verify this supposition, we are to 96 Sorghum vulgare Pers. strain individual plant cadmium total amounts
Counted, high first 10 are followed successively by individual plant cadmium total amount：' CHEDOMBA ' (numbering ' 38 '), ' Dhurra No.7 ' (numberings
' 31 '), ' MN 4138 ' (numbering ' 62 '), ' Juar ' (numbering ' 52 '), ' MALNAL ' (numbering ' 21 '), ' NYTWAL ' (numbering
' 13 '), ' UMM EL TEIMAN ' (numbering ' 18 '), ' Co.1 ' (numbering ' 66 '), ' MN 2089 ' (numbering ' 36 '), ' ETS
3080 ' (numberings ' 72 ').And ' Dhurra No.7 ', ' CHEDOMBA ' and ' MN 4138 ' these three strain individual plant cadmium total amounts are
96 Sorghum vulgare Pers. strain individual plant cadmium total amount highest front three (see Fig. 4), this illustrates the feasibility of this kind of method, in the future can be
Popularization is further confirmed that in field experiment.
Claims (7)
1. a kind of screening technique of the Sorghum Germplasm repaired for cadmium pollution soil, comprises the steps：
1) sorghum seeds are sprouted, the seedling for obtaining is cultivated in the nutritional solution containing cadmium, point root and aerial partss are harvested, respectively
The root and aerial partss of the sorghum seedling collected are dried to constant weight, is then weighed；
2) root dry weight is calculated using fuzzy mathematics Subordinate Function and is subordinate to letter according to the root and the dry weight of aerial partss of Sorghum vulgare Pers. respectively
Numerical value and aerial partss dry weight membership function value, do further according to the root dry weight membership function value and aerial partss of a certain Sorghum vulgare Pers. strain
Weight membership function value calculates the tolerance coefficient of the Sorghum vulgare Pers. strainSo as to carry out the cadmium toleration evaluation of the strain Sorghum vulgare Pers.,
And Sorghum vulgare Pers. strain is divided into into multiple grades；
4) correlation analysiss and path analysiss are carried out, determines that the important references that root dry weight is Sorghum vulgare Pers. strain Cd uptake amount size refer to
Mark, the high strain of Cd uptake amount is filtered out according to the root dry weight of each strain from the relatively strong Sorghum vulgare Pers. strain of toleration, that is, sieve
Select the Sorghum Germplasm that can be used for cadmium pollution soil reparation.
2. method according to claim 1, it is characterised in that：Step 1) in, the nutritional solution is 1/2Hoagland nutrition
Liquid；
Cd ultimate densities are 5-15 μM in the nutritional solution；
The culture is hot-house culture；
The condition of hot-house culture is：25 DEG C of daytime, 20 DEG C of evening, illumination 16h, relative humidity 60%；
The drying is carried out in 70 DEG C of baking oven.
3. method according to claim 1 and 2, it is characterised in that：Step 2) in, the tolerance system of a certain Sorghum vulgare Pers. strain
NumberFor its root dry weight membership function value and the meansigma methodss of aerial partss dry weight membership function value.
4. the method according to any one of claim 1-3, it is characterised in that：Step 2) in, according to tolerance coefficient magnitude
Sorghum vulgare Pers. strain is divided into into five grades, I grades are the most strong strain of cadmium toleration,Ii grade is cadmium tolerance
The stronger strain of property,Ii I grade is the medium strain of cadmium toleration,Iv etc.
Level is the sensitive strain of cadmium,V grades are most sensitive strain,
5. the method according to any one of claim 1-4, it is characterised in that：Step 3) in, the toleration is relatively strong
Sorghum vulgare Pers. strain be I and II grade strains.
6. Sorghum Germplasm the answering in cadmium pollution soil reparation for being filtered out by method any one of claim 1-5
With.
7. application according to claim 6, it is characterised in that：The Sorghum Germplasm be ' CHEDOMBA ', ' Dhurra
No.7 ', ' MN 4138 ', ' Juar ', ' MALNAL ', ' NYTWAL ', ' UMM EL TEIMAN ', ' Co.1 ', ' MN 2089 ' or
‘ETS 3080’。
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108921415A (en) * | 2018-06-25 | 2018-11-30 | 西南石油大学 | A kind of analysis method of oil reservoir stimulation influential effect factor |
| CN113866370A (en) * | 2021-11-16 | 2021-12-31 | 青海师范大学 | Method for identifying heavy metal tolerant plants through morphological indexes |
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| CN1875690A (en) * | 2006-06-30 | 2006-12-13 | 浙江大学 | A method for selecting cadium toxicity-resistant genotype barley |
| CN102172132A (en) * | 2011-01-25 | 2011-09-07 | 湖南农业大学 | Method for screening ramie varieties and method for restoring cadmium contaminated soil by using ramie |
| CN104488430A (en) * | 2014-12-10 | 2015-04-08 | 浙江工商大学 | Application method of nitrogen fertilizer suitable for plantation of vegetable crops in cadmium contaminated field |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1875690A (en) * | 2006-06-30 | 2006-12-13 | 浙江大学 | A method for selecting cadium toxicity-resistant genotype barley |
| CN102172132A (en) * | 2011-01-25 | 2011-09-07 | 湖南农业大学 | Method for screening ramie varieties and method for restoring cadmium contaminated soil by using ramie |
| CN104488430A (en) * | 2014-12-10 | 2015-04-08 | 浙江工商大学 | Application method of nitrogen fertilizer suitable for plantation of vegetable crops in cadmium contaminated field |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108921415A (en) * | 2018-06-25 | 2018-11-30 | 西南石油大学 | A kind of analysis method of oil reservoir stimulation influential effect factor |
| CN108921415B (en) * | 2018-06-25 | 2022-07-08 | 西南石油大学 | Method for analyzing influence factors of oil increasing effect of oil reservoir measures |
| CN113866370A (en) * | 2021-11-16 | 2021-12-31 | 青海师范大学 | Method for identifying heavy metal tolerant plants through morphological indexes |
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