CN106566277A - Preparation method of all-purple potato anthocyanin monomer - Google Patents
Preparation method of all-purple potato anthocyanin monomer Download PDFInfo
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- CN106566277A CN106566277A CN201610124625.8A CN201610124625A CN106566277A CN 106566277 A CN106566277 A CN 106566277A CN 201610124625 A CN201610124625 A CN 201610124625A CN 106566277 A CN106566277 A CN 106566277A
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- Prior art keywords
- anthocyanin
- ncc
- rhizoma solani
- solani tuber
- tuber osi
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
Abstract
The invention discloses a preparation method of an all-purple potato anthocyanin monomer. The method includes the steps of: raw material pretreatment, coarse extraction of all-purple potato anthocyanin, refining of all-purple potato anthocyanin, and preparation of the all-purple potato anthocyanin monomer, etc. The method provided by the invention adopts the "macroporous resin crude purification, solvent extraction and high performance liquid chromatography" three-step approach to realize separation of the all-purple potato anthocyanin monomer, and has the advantages of simple process, simple equipment, controllable operation and low cost, thus being convenient for large-scale production in grassroots units. The invention relates to the high performance liquid chromatography preparation method of the high purity anthocyanin monomer, is especially suitable for providing a standard substance for accurate quantitation of all-purple potato pigment, and solves the high price problem of imported standard substances.
Description
【Technical field】
The present invention relates to the preparation of edible natural pigment, more particularly to a kind of preparation method of NCC Rhizoma Solani tuber osi anthocyanin monomer.
【Background technology】
Color is an important symbol of sensory quality of food, and attracts the key factor of consumer, so while color in food
Plain addition is very few, but the impact to food quality and quality is very big.Food coloring is broadly divided into natural pigment and combination color
Element, wherein, after synthetic dyestuff comes across in the middle of the 19th century, with its unique advantage, including beautiful in colour, good stability, dye
Color power is strong, odorless, tasteless, low price the advantages of, be used widely in food color field quickly.But from Britain (1967
Year), the U.S. (1973), the country such as World Health Organization (WHO) (1984) or mechanism be in succession to synthetic dyestuff (tar colorant) to human body
Safety query, the research of the hazard of contaminant such as arsenic lead that may be brought in building-up process report day by day increases, Yi Jihe
Happened occasionally into pigment by the situation of Use overrun and Use out of range.Therefore, the safe natural pigment of development and application will
It is a kind of inexorable trend.
Natural pigment typically refers to the secondary generation that the material (such as animals and plants material) existed using nature or cultural method production are obtained
Thank to material, carry out certain processing and made by pigment.Due to its have it is safe and reliable, have no toxic side effect, tone it is natural and many
The advantages of feature, widely should obtain along with the development of food industry, medical industry, daily chemical industry and aquaculture etc.
With.And research both domestic and external finds that natural pigment is not only safe, and tone is soft, and some have specific physiology
Activity and pharmacological function, belong to Functional Edible Natural Pigment.Therefore, what the exploitation of natural pigment became at this stage is important
Task.
It is reported that, Japan allows the natural colour for using to have 97 kinds, occupies 90% market share.China's food colour exploitation
It is later and main based on synthetic dyestuff (more than 90%), but with expanding economy and the reinforcement of people's Consciousness of food security, day
So pigment replaces synthetic dyestuff also become main flow.However, the bottleneck problem that the researches and exploitation of natural food colour faces
One of be a lack of a large amount of natural pigment resources with commercial exploitation.Which results in natural food colour price partially expensive,
Also its popularization in application is limited to a great extent.Vast territory and abundant resources for China, edible plants resource very abundant, therefore,
It will solve the key that natural pigment carrys out source problem to find suitable edible plants development of resources natural pigment.
NCC Rhizoma Solani tuber osi is a kind of special Rhizoma Solani tuber osi rare variety of integration of edible and medicinal herbs, its present black purple the reason for be to be rich in
Delphindin (purple) anthocyanidin.Anthocyanidin is the disease preventing and treating having now been found that, safeguard human health it is most direct, most effective,
One of safest free radical scavenger, with anticancer, prevent and treat hypertension, cardiovascular and cerebrovascular disease, defying age, skin maintenance and
Improve the plurality of health care functions such as body immunity.For NCC Rhizoma Solani tuber osi anthocyanin, which to be studied activity occurs in human body
The situation of effect, the acquisition of monomeric substance is crucial, so monomer not only serves as natural food pigment, it is also possible to as research
Purposes；NCC Rhizoma Solani tuber osi pigment is quantitative at present, does not have unified standard substance, after having monomer, can quantitatively develop one kind
Standard method；Meanwhile, the monomer anthocyanin for obtaining can be passed through, its relation with health is more in depth studied.So,
The preparation of NCC Rhizoma Solani tuber osi anthocyanin monomer, for food processing industry and health care industry, all with highly important meaning
Justice.
The existing report with regard to NCC Rhizoma Solani tuber osi pigment, essentially consists in its pigment crude extract extracting method of concern and monomer component composition.
But from precisely quantitative to which and play bioactive substance and be accurately positioned angle to consider, its high-purity monomer obtained, is downstream
The exploitation of Related product is originated there is provided direct material, with more realistic meaning.At present, for NCC Rhizoma Solani tuber osi anthocyanin list
The preparation of body, has no report.And with regard to the preparation of other natural pigment monomers, now mainly adopt high speed adverse current chromatogram and preparation solution
The separation means that phase chromatograph combines.But this set of system price is expensive, not all scientific research institutions and enterprise can buy
This set of system.Simultaneously for the closely similar pigment monomer of property, on preparative liquid chromatograph, due to sample size it is big, and
Preparative hplc post post effect is relatively low, often cannot be separated well, and then cannot obtain monomeric substance.The present invention can be with
The deficiency of existing isolation technics is made up, by the thick extraction and extraction of early stage, it is ensured that the sample purity before upper prop is higher, Jin Ertong
Cross common efficient liquid phase and realize the separation of anthocyanin monomer.This technological means is all easy in general scientific research institutions and enterprise
Implement.
【The content of the invention】
It is an object of the invention to the above-mentioned deficiency of customer service prior art, there is provided a kind of good separating effect, the NCC soil of low cost
The preparation method of bean anthocyanin monomer.
For achieving the above object, the present invention provide NCC Rhizoma Solani tuber osi anthocyanin monomer preparation method, it is characterised in that include as
Lower step：
(1) pretreatment of raw material：Taking and peeling being cleaned without the Rhizoma Solani tuber osi for rotting, 40-50 DEG C of drying is beaten powder, cross 40-80 mesh sieves,
It is standby；
(2) the thick extraction of NCC Rhizoma Solani tuber osi anthocyanin：The mealy potato extracting solution extraction that step (1) is obtained is taken, lixiviating solution is taken
10-15min is centrifuged in 4000-8000r/min, thing and supernatant is precipitated, the same terms is pressed in precipitate and adds said extracted
Liquid repeats to extract 1 time, merges 2 supernatant and is NCC Rhizoma Solani tuber osi anthocyanin crude extract；
(3) NCC Rhizoma Solani tuber osi anthocyanin is refined：The NCC Rhizoma Solani tuber osi anthocyanin crude extract that step (2) is obtained is with 1-3mL/min
Flow velocity through the adsorption column filled with macroporous resin, NCC Rhizoma Solani tuber osi anthocyanin by macroporous resin adsorption saturation, with water or
The HCl solution of 1.2mmol/L is with the flow velocity washing macroporous resin column of 1-3mL/min, then is 60%-80% with mass percent concentration
Ethanol solution with the flow velocity eluting of 1-3mL/min by the NCC Rhizoma Solani tuber osi anthocyanin of macroporous resin adsorption, collect eluent；
The 1/3 of original volume is evaporated under the conditions of 50 DEG C, NCC Rhizoma Solani tuber osi anthocyanin concentrated solution is obtained；Concentrated solution lyophilization is obtained
Aubergine NCC Rhizoma Solani tuber osi anthocyanin powder.
(4) preparation of NCC Rhizoma Solani tuber osi anthocyanin monomer：With the formic acid solution that percent by volume is 0.3%:Acetonitrile is according to volume ratio
Equal to 9:Anthocyanin corase meal is dissolved by 1 solvent, is added the ethyl acetate of 1-2 times of volume to extract 3 times, is abandoned ethyl acetate layer,
Pars pigmentosa is separated using high performance liquid chromatography, collects monomer, collection liquid is evaporated under the conditions of 50 DEG C the 1/3 of original volume,
Lyophilization, obtains final product NCC Rhizoma Solani tuber osi anthocyanin monomer；
Extracting solution described in step (2) is the aqueous solution of the aqueous solution or acidified with citric acid of hydrochloric acid acidifying, the wherein concentration of HCl
For 0.1mol/L-0.3mol/L, or the mass percent concentration of citric acid is 2%-4%；
Preferably, when the extracting solution is added in step (2), solid-liquid ratio is 1g:60mL-1g:80mL, extraction temperature are 50-70 DEG C,
Extraction time is 1-2h.
Preferably, the macroporous resin described in step (3) is AB-8, D101, DM130 or X-5.
Preferably, the method for separating liquid phase chromatography described in step (4) is：Mobile phase A is formic acid that mass percent is 0.3%
Solution, Mobile phase B are acetonitrile；According to time 0min, 5min, 13min, 17min, 20min, 21min, 25min,
Correspondence mobile phase A accounts for 90%, 88%, 79%, 76%, 65%, 90%, 90% gradient elution by volume respectively；Chromatograph
Post is C18Chromatographic column, specification be 4.6 × 150mm, 5 μm；Flow velocity 1mL/min；100 μ L of sampling volume.
The inventive method has the advantage that：
(1) present invention realizes NCC using the three-step approach of " the thick purification+solvent extraction+high performance liquid chromatography separation of macroporous resin "
The separation of Rhizoma Solani tuber osi anthocyanin monomer.Simple process, equipment are simple, operate controllable, low cost, are easy in establishment size
Production.
(2) the present invention relates to high performance liquid chromatography prepares high-purity anthocyanin monomer, it is particularly suitable for as NCC Rhizoma Solani tuber osi pigment
Precisely quantitative to provide standard substance, this will solve the expensive problem of purchase import standard substance.
【Description of the drawings】
Fig. 1 is the preparation technology flow process of the NCC Rhizoma Solani tuber osi anthocyanin monomer of the present invention.
【Specific embodiment】
Technical scheme is described in detail in conjunction with drawings and Examples.It should be understood that following examples are merely to illustrate this
Bright rather than restriction the scope of the present invention.Without departing from the spirit and substance of the case in the present invention, to step of the present invention or condition
The modification made or replacement, belong to the scope of the present invention.
If not specializing, the conventional meanses that technological means used are well known to those skilled in the art in embodiment.
Embodiment 1
Referring to Fig. 1, taking 10g and peeling being cleaned without rotten NCC Rhizoma Solani tuber osi, 50 DEG C of drying are beaten powder, cross 50 mesh sieves, obtain 2g Rhizoma Solani tuber osis
Powder.NCC mealy potato 2g is taken, is 1g by solid-liquid ratio:60mL adds the HCl solution of 0.3mol/L, extracts 1.5h in 70 DEG C,
4000r/min is centrifuged 10min.Repeat said extracted 1 time, merge 2 supernatant and be NCC Rhizoma Solani tuber osi anthocyanin crude extract.
By NCC Rhizoma Solani tuber osi anthocyanin crude extract with the flow velocity of 1mL/min through the adsorption column filled with macroporous resin AB-8, NCC soil
Bean anthocyanin is washed macroporous resin column with the flow velocity of 1mL/min with the HCl solution of 1.2mmol/L by macroporous resin adsorption saturation,
Afterwards with the ethanol solution that mass percent concentration is 60% with the flow velocity eluting of 1mL/min by the NCC Rhizoma Solani tuber osi of macroporous resin adsorption
Anthocyanin, collects eluent；Eluent is evaporated to into the 1/3 of original volume under the conditions of 50 DEG C, NCC Rhizoma Solani tuber osi flower is obtained
Blue or green glycosides concentrated solution；Concentrated solution lyophilization is obtained into aubergine anthocyanin corase meal 13.8mg.With 4mL solvents (wherein volume hundred
Divide than the formic acid solution for 0.3%:Acetonitrile is equal to 9 according to volume ratio:1) 13.8mg anthocyanins corase meal is dissolved, with 1 times of volume
Ethyl acetate extract 3 times, abandon ethyl acetate layer, pars pigmentosa is separated using high performance liquid chromatography, collects monomer, will collection
Liquid is evaporated to the 1/3 of original volume under the conditions of 50 DEG C, after lyophilization, obtains anthocyanin monomer 1 and monomer 2 is respectively 2.8mg
And 0.7mg, purity respectively 99.78% and 99.71%.Above-mentioned method for separating liquid phase chromatography is：Mobile phase A is mass percent
It is 0.3% formic acid solution, Mobile phase B is acetonitrile；According to time 0min, 5min, 13min, 17min, 20min, 21min,
25min, correspondence mobile phase A account for 90%, 88%, 79%, 76%, 65%, 90%, 90% gradient elution by volume respectively；
Chromatographic column is C18Chromatographic column, specification be 4.6 × 150mm, 5 μm；Flow velocity 1mL/min；100 μ L of sampling volume.
Embodiment 2
Referring to Fig. 1, taking 10g and peeling being cleaned without rotten NCC Rhizoma Solani tuber osi, 50 DEG C of drying are beaten powder, cross 60 mesh sieves, obtain 2g native
Semen Glycines powder, takes NCC mealy potato 2g, by solid-liquid ratio 1g:80mL adds the aqueous citric acid solution that mass percent concentration is 4%,
2h, 4000r/min centrifugation 15min are extracted in 60 DEG C.Repeatedly extract 1 time as stated above, merge 2 supernatant as black
Buddha's warrior attendant Rhizoma Solani tuber osi anthocyanin crude extract, by NCC Rhizoma Solani tuber osi anthocyanin crude extract with the flow velocity of 3mL/min through filled with macroporous resin
The adsorption column of AB-8, NCC Rhizoma Solani tuber osi anthocyanin are washed macropore with the flow velocity of 1mL/min with water by macroporous resin adsorption saturation
Resin column, after with the ethanol solution that mass percent concentration is 80% with the flow velocity eluting of 2mL/min by the black of macroporous resin adsorption
Buddha's warrior attendant Rhizoma Solani tuber osi anthocyanin, collects eluent；Eluent is evaporated to into the 1/3 of original volume under the conditions of 50 DEG C, dark fund is obtained
Firm Rhizoma Solani tuber osi anthocyanin concentrated solution；Concentrated solution lyophilization is obtained into aubergine anthocyanin corase meal 12.6mg.With 3mL solvent (its
Middle percent by volume is 0.3% formic acid solution:Acetonitrile is equal to 9 according to volume ratio:1) 12.6mg anthocyanins corase meal is dissolved,
Extracted 3 times with 2 times of volume of ethylacetate, abandon ethyl acetate layer, pars pigmentosa is separated using high performance liquid chromatography.Collect monomer,
Collection liquid is evaporated to into the 1/3 of original volume, after lyophilization under the conditions of 50 DEG C, 2 points of anthocyanin monomer 1 and monomer is obtained
Not Wei 2.7mg and 0.9mg, purity be respectively 99.85% and 99.79%.Above-mentioned method for separating liquid phase chromatography is：Mobile phase A
It is 0.3% formic acid solution for mass percent, Mobile phase B is acetonitrile；According to 0min, 5min, 13min, 17min,
20min, 21min, 25min mobile phase A accounts for 90%, 88%, 79%, 76%, 65%, 90%, 90% by volume respectively
Gradient elution；Chromatographic column be C18 chromatographic columns, specification be 4.6 × 150mm, 5 μm；Flow velocity 1mL/min；Sampling volume
100μL。
Embodiment 3
Referring to Fig. 1, taking 100g and peeling being cleaned without rotten NCC Rhizoma Solani tuber osi, 50 DEG C of drying are beaten powder, cross 80 mesh sieves, obtain 20g
Mealy potato.NCC mealy potato 20g is taken, by solid-liquid ratio 1g:70mL adds the HCl solution of 0.3mol/L, extracts 2h in 50 DEG C,
8000r/min is centrifuged 10min.Repeat to extract 1 time, merge 2 supernatant and be NCC Rhizoma Solani tuber osi anthocyanin crude extract, will be black
Buddha's warrior attendant Rhizoma Solani tuber osi anthocyanin crude extract is with the flow velocity of 3mL/min through the adsorption column filled with macroporous resin D101, NCC Rhizoma Solani tuber osi flower
Blue or green glycosides is washed macroporous resin column with the flow velocity of 3mL/min with the HCl solution of 1.2mmol/L, is used afterwards by macroporous resin adsorption saturation
Mass percent concentration be 70% ethanol solution with the flow velocity eluting of 2mL/min by the NCC Rhizoma Solani tuber osi cyanine of macroporous resin adsorption
Glycosides, collects eluent；Eluent is evaporated to into the 1/3 of original volume under the conditions of 50 DEG C, NCC Rhizoma Solani tuber osi anthocyanin is obtained
Concentrated solution；Concentrated solution lyophilization is obtained into aubergine anthocyanin corase meal 135.6mg.With 10mL solvents (wherein volume hundred
Divide than the formic acid solution for 0.3%:Acetonitrile is equal to 9 according to volume ratio:1) 135.6mg anthocyanins corase meal is dissolved, with 1 times of body
Product ethyl acetate is extracted 3 times, abandons ethyl acetate layer, and pars pigmentosa is separated using high performance liquid chromatography.Monomer is collected, will be collected
Liquid is evaporated to the 1/3 of original volume under the conditions of 50 DEG C, after lyophilization, obtains anthocyanin monomer 1 and monomer 2 is respectively
32.5mg and 12.2mg, purity difference 99.69% and 99.77%.Above-mentioned method for separating liquid phase chromatography is：Mobile phase A is quality
Percentage ratio is 0.3% formic acid solution, and Mobile phase B is acetonitrile；According to time 0min, 5min, 13min, 17min, 20min,
21min, 25min, correspondence mobile phase A account for 90%, 88%, 79%, 76%, 65%, 90%, 90% by volume respectively
Gradient elution；Chromatographic column is C18Chromatographic column, specification be 4.6 × 150mm, 5 μm；Flow velocity 1mL/min；100 μ L of sampling volume.
Embodiment 4
Referring to Fig. 1, taking 100g and peeling being cleaned without rotten NCC Rhizoma Solani tuber osi, 50 DEG C of drying are beaten powder, cross 40 mesh sieves, obtain 20g
Mealy potato.NCC mealy potato 20g is taken, by solid-liquid ratio 1g:60mL adds the aqueous citric acid solution that mass percent concentration is 2%,
1.5h, 4000r/min centrifugation 10min are extracted in 60 DEG C.Repeat to extract 1 time, merge 2 supernatant and be NCC Rhizoma Solani tuber osi flower
Blue or green glycosides crude extract.By NCC Rhizoma Solani tuber osi anthocyanin crude extract with the flow velocity of 1mL/min through the absorption filled with macroporous resin D101
Post, NCC Rhizoma Solani tuber osi anthocyanin are washed macroporous resin column with the flow velocity of 1mL/min with water, are used afterwards by macroporous resin adsorption saturation
Mass percent concentration be 80% ethanol solution with the flow velocity eluting of 1mL/min by the NCC Rhizoma Solani tuber osi cyanine of macroporous resin adsorption
Glycosides, collects eluent；Eluent is evaporated under the conditions of temperature 50 C the 1/3 of original volume, NCC Rhizoma Solani tuber osi flower is obtained
Blue or green glycosides concentrated solution；Concentrated solution lyophilization is obtained into aubergine anthocyanin corase meal 127.3mg, with 8mL solvents (wherein body
Product percentage ratio is 0.3% formic acid solution:Acetonitrile is equal to 9 according to volume ratio:1) 127.3mg anthocyanins corase meal is dissolved, uses 2
Times volume of ethylacetate is extracted 3 times, abandons ethyl acetate layer, and pars pigmentosa is using high performance liquid chromatography separation.Monomer is collected, will
Collection liquid is evaporated to the 1/3 of original volume under the conditions of 50 DEG C, after lyophilization, obtains anthocyanin monomer 1 and monomer 2 is distinguished
For 31.3mg and 11.2mg, purity respectively 99.75% and 99.77%.Above-mentioned method for separating liquid phase chromatography is：Mobile phase A is
Mass percent is 0.3% formic acid solution, and Mobile phase B is acetonitrile；According to time 0min, 5min, 13min, 17min,
20min, 21min, 25min, correspondence mobile phase A accounts for 90% by volume respectively, 88%, 79%, 76%, 65%, 90%,
90% gradient elution；Chromatographic column is C18Chromatographic column, specification be 4.6 × 150mm, 5 μm；Flow velocity 1mL/min；Sample introduction body
100 μ L of product.
Claims (4)
1. a kind of preparation method of NCC Rhizoma Solani tuber osi anthocyanin monomer, it is characterised in that comprise the steps：
(1) pretreatment of raw material：Taking and peeling being cleaned without the Rhizoma Solani tuber osi for rotting, 40-50 DEG C of drying is beaten powder, cross 40-80 mesh sieves, standby；
(2) the thick extraction of NCC Rhizoma Solani tuber osi anthocyanin：Take the mealy potato that step (1) obtains to be extracted with extracting solution, take lixiviating solution and 10-15min is centrifuged in 4000-8000r/min, it is precipitated thing and supernatant, the same terms are pressed in precipitate adds said extracted liquid to repeat to extract 1 time, merges 2 supernatant and is NCC Rhizoma Solani tuber osi anthocyanin crude extract；
(3) NCC Rhizoma Solani tuber osi anthocyanin is refined：The NCC Rhizoma Solani tuber osi anthocyanin crude extract that step (2) is obtained is with the flow velocity of 1-3mL/min through the adsorption column filled with macroporous resin, NCC Rhizoma Solani tuber osi anthocyanin is by macroporous resin adsorption saturation, macroporous resin column is washed with the flow velocity of 1-3mL/min with the HCl solution of water or 1.2mmol/L, again with the ethanol solution that mass percent concentration is 60%-80% with the flow velocity eluting of 1-3mL/min by the NCC Rhizoma Solani tuber osi anthocyanin of macroporous resin adsorption, collect eluent；The 1/3 of original volume is evaporated under the conditions of 50 DEG C, NCC Rhizoma Solani tuber osi anthocyanin concentrated solution is obtained；Concentrated solution lyophilization is obtained into aubergine NCC Rhizoma Solani tuber osi anthocyanin corase meal.
(4) preparation of NCC Rhizoma Solani tuber osi anthocyanin monomer：With the formic acid solution that percent by volume is 0.3%:Acetonitrile is equal to 9 according to volume ratio:The anthocyanin corase meal dissolving that step (3) is obtained by 1 solvent, the ethyl acetate of 1-2 times of volume is added to extract 3 times, abandon ethyl acetate layer, pars pigmentosa is separated using high performance liquid chromatography, collect monomer, collection liquid is evaporated to into the 1/3 of original volume under the conditions of 50 DEG C, lyophilization obtains final product NCC Rhizoma Solani tuber osi anthocyanin monomer；
Extracting solution described in step (2) be hydrochloric acid acidifying aqueous solution or acidified with citric acid aqueous solution, wherein the concentration of HCl be 0.1mol/L-0.3mol/L, or the mass percent concentration of citric acid be 2%-4%.
2. the preparation method of a kind of NCC Rhizoma Solani tuber osi anthocyanin monomer according to claim 1, it is characterised in that：When the extracting solution is added in step (2), solid-liquid ratio is 1g:60mL-1g:80mL, extraction temperature are 50-70 DEG C, and extraction time is 1-2h.
3. the preparation method of a kind of NCC Rhizoma Solani tuber osi anthocyanin monomer according to claim 1, it is characterised in that：Macroporous resin described in step (3) is AB-8, D101, DM130 or X-5.
4. the preparation method of a kind of NCC Rhizoma Solani tuber osi anthocyanin monomer according to claim 1, it is characterised in that：Method for separating liquid phase chromatography described in step (4) is：Mobile phase A is the formic acid solution that mass percent is 0.3%, and Mobile phase B is acetonitrile；According to time 0min, 5min, 13min, 17min, 20min, 21min, 25min, correspondence mobile phase A accounts for 90%, 88%, 79%, 76%, 65%, 90%, 90% gradient elution by volume respectively；Chromatographic column is C18Chromatographic column, specification be 4.6 × 150mm, 5 μm；Flow velocity 1mL/min；100 μ L of sampling volume.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870685A (en) * | 2010-06-29 | 2010-10-27 | 湖南农业大学 | Method for extracting anthocyanin from purple potatoes |
| CN103145681A (en) * | 2013-03-15 | 2013-06-12 | 中国农业科学院农产品加工研究所 | Method for extracting anthocyanin |
| CN103626814A (en) * | 2013-12-09 | 2014-03-12 | 中国科学院西北高原生物研究所 | Method for separating anthocyanins monomer from lycium ruthenicum fruits |
| CN104356106A (en) * | 2014-10-21 | 2015-02-18 | 山东省科学院生物研究所 | Extraction and purification method of purple potato anthocyanin |
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2016
- 2016-03-04 CN CN201610124625.8A patent/CN106566277A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870685A (en) * | 2010-06-29 | 2010-10-27 | 湖南农业大学 | Method for extracting anthocyanin from purple potatoes |
| CN103145681A (en) * | 2013-03-15 | 2013-06-12 | 中国农业科学院农产品加工研究所 | Method for extracting anthocyanin |
| CN103626814A (en) * | 2013-12-09 | 2014-03-12 | 中国科学院西北高原生物研究所 | Method for separating anthocyanins monomer from lycium ruthenicum fruits |
| CN104356106A (en) * | 2014-10-21 | 2015-02-18 | 山东省科学院生物研究所 | Extraction and purification method of purple potato anthocyanin |
Non-Patent Citations (1)
| Title |
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| 王仁雷 等: "紫马铃薯花色苷的提取纯化与鉴定", 《食品科学》 * |
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Application publication date: 20170419 |