CN106565448B - A method of isolating and purifying 7- hydroxy tropolone from bacterial supernatant - Google Patents
A method of isolating and purifying 7- hydroxy tropolone from bacterial supernatant Download PDFInfo
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- CN106565448B CN106565448B CN201510662595.1A CN201510662595A CN106565448B CN 106565448 B CN106565448 B CN 106565448B CN 201510662595 A CN201510662595 A CN 201510662595A CN 106565448 B CN106565448 B CN 106565448B
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- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
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Abstract
The method that the invention discloses a kind of to isolate and purify 7- hydroxy tropolone from bacterial supernatant.The method of the present invention is the following steps are included: it is CCTCC NO:M 2015481 that (1), which collects East Lake pseudomonad HT(deposit number) fermentation liquid, supernatant is filtered, obtain supernatant of bacteria solution after centrifugation；(2) supernatant of bacteria solution is extracted with ethyl acetate, retains the water phase of lower layer；The pH of water phase is adjusted to acidity, water phase is extracted with ethyl acetate again, collects upper organic phase；(3) by the ethyl acetate evaporated under reduced pressure in organic phase, remaining substance is dissolved in dehydrated alcohol；(4) chromatogram purification is carried out using Sephadex LH-20, mobile phase is dehydrated alcohol, and collection has the active component of chela iron, and dehydrated alcohol evaporated under reduced pressure is obtained 7- hydroxy tropolone.Purity can be obtained through the invention up to 90% or more 7- hydroxy tropolone, the rate of recovery is greater than 60%.
Description
Technical field
The invention belongs to bioengineering, technological field of biochemistry, and in particular to one kind is isolated and purified from bacterial supernatant
The method of 7- hydroxy tropolone.
Background technique
7- hydroxy tropolone class compound is a kind of natural products for belonging to troponoid, very
Many aspects show polynary bioactivity.Due to a hydroxyl more than the ortho position in ketone group, so that 7- hydroxyl cycloheptatriene
Phenolic ketone shows stronger inhibitory activity relative to tropolone.It is some researches show that 7- hydroxy tropolone and its
Derivative β-ramie thujaplicinol (β-thujaplicinol), 3,7- dihydroxy tropolone, puberulic acid (puberulic
Acid), puberulonic acid (puberulonic acid) can be used as the potential treatment drug of a variety of diseases.7- hydroxyl cycloheptyl three
Alkene phenolic ketone shows stronger inhibitory activity for gram-positive bacterium, as staphylococcus aureus (Staphylococcus aureus) and micrococcus luteus (Micrococcus luteus) MIC value be 90 μM.7- hydroxy tropolone for
The IC of B16 melanoma cells50Value is 2.2 μM.7- hydroxy tropolone has anti-malarial activity, for chlorine
Quinoline resistance plasmodium falciparum (Plasmodium falciparum) K1 bacterial strain IC50Value is 6.4 μM.Alpha-hydroxy cycloheptatriene
Tropolones compound has inhibitory activity for diatomic metalloenzyme, and 7- hydroxy tropolone is for diatomic metalloenzyme inositol
Monophosphate enzyme (IMPase), alkaline phosphatase (APase), dopamine tryptophan side-chain alpha (DBM) IC50Value is respectively 8,60,3 μM.
7- hydroxy tropolone can also inhibit 2 "-O- adenine transferase of aminoglycoside in bacterium, can be used as aminoglycoside
The enhancement of antibiotic is total to drug.
In nineteen fifty-three, Japanese Scientists Nozoe is precursor using tropolone, synthesizes 7- by potassium persulfate oxidation
Hydroxy tropolone.Nineteen eighty-two American scientist Kirst synthesizes 7- hydroxyl cycloheptatriene phenol using identical synthetic method
Ketone, separation method are that reaction mixture is adjusted to pH 4, ether extraction removal unreacted tropolone, and water phase is adjusted to pH
1.9, steam bath heating is extracted after being adjusted to pH 4.2 with sodium hydroxide with ether.After ethereal extract is evaporated, product is with to flow point
Match system isolates and purifies, and solvent system is chloroform-toluene-methanol-water (15:15:23:7).Final 25 g tropolone can
Receive to obtain 3.8 g7- hydroxy tropolones.7- hydroxy tropolone is by American scientist at first in microorganism
Allen bedroom room river streptomycete (Streptomyces neyagawaensis) fermentation liquid in that there is wide spectrum to resist is micro- as a kind of
What the Antibiotics separation of bioactivity was purified.They are chromatographed using HP-20 and Sephadex G-10 column in bedroom room river strepto-
Bacterium (Streptomyces neyagawaensis) 7- hydroxy tropolone is extracted in fermentation liquid, purity is about 25%.It
Also isolated 7- hydroxy tropolone, separation method used are Germany scientist Korth from a pseudomonas afterwards
Culture supernatant is adjusted to pH 3, is extracted with dichloromethane three times, residue liter will be made at 100 DEG C after organic phase solvent evaporated
China's crystallization obtains 7- hydroxy tropolone product.
7- hydroxy tropolone has very big pharmaceutical potential, and main source is chemical synthesis at present, but chemical synthesis
The problems such as there are inflammable, explosive, harmful reagents in method.Existing microbial fermentation extracts 7- hydroxy tropolone and also deposits
It is not high in yield, purity, the problems such as rate of recovery is low.
Summary of the invention
The method that the purpose of the present invention is to provide a kind of to isolate and purify 7- hydroxy tropolone from bacterial supernatant,
This method is suitble to industrial production high-purity 7- hydroxy tropolone.
The purpose of the invention is achieved by the following technical solution:
A method of isolating and purifying 7- hydroxy tropolone from bacterial supernatant, comprising the following steps:
(1) collect East Lake pseudomonad (Pseudomonas donghuensis) HT fermentation liquid, supernatant is filtered after centrifugation
Remaining thallus and insoluble matter are removed, supernatant of bacteria solution is obtained.East Lake pseudomonad HT is preserved in Chinese Typical Representative culture guarantor
Hiding center (preservation date: on August 6th, 2015, preservation address: the Chinese Wuhan Wuhan University), classification naming isPseudomonas donghuensisHT, deposit number are CCTCC NO:M 2015481.
(2) supernatant of bacteria solution is extracted with ethyl acetate, to remove hydrophobic impurity, retains the water phase of lower layer.By water
The pH of phase is adjusted to acidity, and water phase is extracted with ethyl acetate again, collects upper organic phase.
(3) by the ethyl acetate evaporated under reduced pressure in organic phase, remaining substance is dissolved in dehydrated alcohol.
(4) chromatogram purification is carried out using Sephadex LH-20, mobile phase is dehydrated alcohol.Collection has active group of chela iron
Point, the dehydrated alcohol evaporated under reduced pressure of collection having in the active component of chela iron is crystallized to obtain 7- hydroxy tropolone crystalline substance
Body.
Centrifugal condition as described in step (1) is preferred are as follows: 8000-10000 rpm is centrifuged 25-30 min.
Acidity described in step (2) is preferably pH=2-3.
The pH of water phase is preferably adjusted to pH=2-3 with hydrochloric acid in step (2).
The amount of the ethyl acetate of extraction supernatant of bacteria solution is preferably the 1/2 of supernatant of bacteria solution volume in step (2), preferably extracts two
It is secondary.
The amount of the ethyl acetate of aqueous phase extracted is preferably the 1/2 of water phase volume in step (2), is preferably extracted twice.
Step (2) is preferred are as follows: is extracted twice supernatant of bacteria solution with the ethyl acetate of its 1/2 volume, retains the water phase of lower layer.
The pH of water phase is adjusted to pH=2-3 with hydrochloric acid, water phase is extracted twice with the ethyl acetate of its 1/2 volume again, collects upper layer
Organic phase.
Evaporated under reduced pressure described in step (3) and (4) preferably uses Rotary Evaporators to depressurize steaming in 40-42 DEG C of water-bath
It is dry.
The invention has the following advantages that
The present invention is starting strain using East Lake pseudomonad HT, is gone out by microbial fermentation and contains 7- hydroxyl cycloheptatriene phenol
The fermentation liquid of ketone, the problems such as avoiding inflammable, explosive, harmful reagent in chemical synthesis process.Fermentation liquid is passed through into centrifugal filtration
Supernatant, ethyl acetate extraction are taken, purity can be obtained up to 90% or more 7- hydroxy tropolone in the simple procedure of column chromatography
Crystalline product.Be related to instrument reagent simply easily to realize, avoid the participation of some cumbersome techniques and toxic reagent, more economically with
Environmental protection.
The amount that East Lake pseudomonad HT produces 7- hydroxy tropolone is about 80 mg/L.Every liter of bacterium solution through the invention
50-60 mg 7- hydroxy tropolone can be obtained in supernatant, and the rate of recovery is greater than 60%.
Detailed description of the invention
Fig. 1 is the ultraviolet-visible spectrogram of the 7- hydroxy tropolone of purification.
Fig. 2 is the high-efficient liquid phase chromatogram of the 7- hydroxy tropolone of purification, and A: Detection wavelength is 258 nm, B:
Detection wavelength is 330 nm, C: Detection wavelength is 375 nm.
Specific embodiment
Further detailed description is done to the present invention below with reference to examples and drawings, but embodiments of the present invention are unlimited
In this.
Separation, purifying and the identification of 1 7- hydroxy tropolone of embodiment
(1) extraction and purification of 7- hydroxy tropolone
Cultivating East Lake pseudomonad HT and purifying the culture medium of 7- hydroxy tropolone is MKB fluid nutrient medium.Often
It rises and contains ingredient: 15 mL glycerol in MKB fluid nutrient medium, 3.28 g, tri- water dipotassium hydrogen phosphate, 5 g acid hydrolyzed caseins, 2.5
G epsom salt, pH 7-7.2；Iron ion and ferrous ion total content are not higher than 1 μM in MKB fluid nutrient medium.East Lake is false single
Born of the same parents bacterium HT is preserved in China typical culture collection center (preservation date: on August 6th, 2015, preservation address: the Chinese Wuhan
Wuhan University), classification naming isPseudomonas donghuensisHT, deposit number are CCTCC NO:M
2015481。
1) East Lake pseudomonad HT is seeded in the test tube containing 5 mL liquid MKB culture mediums, 30 DEG C of shaking table concussion trainings
It supports and is activated overnight.
2) bacterium solution after overnight incubation is pressed into 1%(V/V) inoculum concentration switching in containing 50 mL liquid MKB culture mediums
In 250mL triangle shake bottle, 30 DEG C of 12 h of shaking table shake culture.
3) large-scale freezing 10000 rpm of Centrifuge Room temperature of the bacterium solution after 12 h of culture is centrifuged 30 min, collection supernatant.
Supernatant, which is filtered, by 0.22 μm of millipore millipore filter removes remaining thallus and insoluble matter.
4) 1/2 ethyl acetate of filtered supernatant supernatant volume is extracted twice, it is hydrophobic miscellaneous to remove
Matter retains the water phase of lower layer.The pH of water phase is adjusted to pH=2 with concentrated hydrochloric acid, the pH water phase for being adjusted to 2 is used into water phase volume again
1/2 ethyl acetate is extracted twice, and 7- hydroxy tropolone enters in ethyl acetate at this time, retains upper organic phase.
By the ethyl acetate Rotary Evaporators in organic phase in 40 DEG C of water-baths evaporated under reduced pressure, bottom of bottle will appear faint yellow needle at this time
Shape crystal.Faint yellow acicular crystal is dissolved in 10 ml dehydrated alcohols and carries out chromatogram purification.
5) chromatogram purification is carried out using Sephadex LH-20, mobile phase is dehydrated alcohol.Sephadex LH-20 used
13 cm of column pillar height, 1 cm of column diameter.Each applied sample amount is 200 μ L.The chela iron activity of component is collected using CAS detection liquid measurement,
Having the active component of chela iron is the component containing 7- hydroxy tropolone, will have the active Fraction collection of chela iron to get off.
6) by collection have the active component Rotary Evaporators of chela iron in 40 DEG C of water-baths evaporated under reduced pressure crystallization to get
To 7- hydroxy tropolone crystal after purification.
(2) identification of 7- hydroxy tropolone
The 7- hydroxy tropolone isolated and purified is subjected to ultraviolet-visible spectrum and high performance liquid chromatography detection, knot
Fruit shows that report is consistent in separated purifying 7- hydroxy tropolone ultraviolet-visible spectral characteristic and document, and purity reaches
90% or more, 50-60 mg 7- hydroxy tropolone can be obtained through above method extraction and purification in every liter of supernatant of bacteria solution.7-
The testing result of hydroxy tropolone is as follows:
1) ultraviolet-visible spectral detection, spectrophotometer model shimadzu UV 2550 used.Testing result
As shown in Figure 1, the ultraviolet-visible spectrum of purification of samples has absorption peak in 258,330,375,365 nm, the absorption at 258 nm
Peak represents the absorption of unsaturated bond, and the absorption peak and conjugated structure at 330,375,365 nm are related.The ultraviolet of purification of samples can
Light-exposed spectrum and chemically synthesized 7- hydroxy tropolone ultraviolet-visible spectrum and document (Yoshinaga Oka,
Shigeki Matsuo. 1956. Spectrophotometric Studies on Organometallic Complexes
Used in Analytical Chemistry on the Germanium Complex with 3-
hydroxytropolone. Tohoku University. Ser. A, Physics, chemistry and
Metallurgy. 8,532-539.) in report it is consistent.According to the research of Yoshinaga Oka, 1 × 10-4The 7- hydroxyl ring of M
Absorption value of the heptantriene phenolic ketone in a neutral environment at 330 nm is about 0.9 or so, and in the above-mentioned training of East Lake pseudomonad HT
Supporting absorption maximum of the supernatant of 12h at 330 nm is about 5-6, by 7- hydroxy tropolone is calculated in East Lake vacation
The yield of monad HT bacterial strain supernatant is about 80 mg/L, i.e., the rate of recovery of above-mentioned isolation and purification method is 60% or more.
2) high performance liquid chromatography detection, high performance liquid chromatograph type number used are Agilent 1100.Chromatography used
Column is 4.6 × 250 mm of Eclipse plus C18 reverse-phase chromatographic column, and mobile phase is methanol: water=4:6(V/V), flow phase velocity
Rate is set as 1 mL/min, and each applied sample amount is 10 μ L, 258,330,375 nm of Detection wavelength.Have as the result is shown one it is bright
Aobvious main elution peak (Fig. 2), illustrates that the composition in purification of samples is single, and 7- hydroxy tropolone purity is up to 90% or more.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (8)
1. a kind of method for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, it is characterised in that including following step
It is rapid:
(1) East Lake pseudomonad HT fermentation liquid is collected, supernatant is filtered after centrifugation and removes remaining thallus and insoluble matter, obtains bacterium solution
Supernatant；The deposit number of East Lake pseudomonad HT is CCTCC NO:M 2015481；
(2) supernatant of bacteria solution is extracted with ethyl acetate, retains the water phase of lower layer；The pH of water phase is adjusted to acidity, again by water phase
It is extracted with ethyl acetate, collects upper organic phase；
(3) by the ethyl acetate evaporated under reduced pressure in organic phase, remaining substance is dissolved in dehydrated alcohol；
(4) chromatogram purification being carried out using Sephadex LH-20, mobile phase is dehydrated alcohol, and collection has the active component of chela iron,
Dehydrated alcohol evaporated under reduced pressure is obtained into 7- hydroxy tropolone.
2. the method according to claim 1 for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, feature
It is: centrifugal condition as described in step (1) are as follows: 8000-10000 rpm is centrifuged 25-30 min.
3. the method according to claim 1 for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, feature
Be: acidity described in step (2) is pH=2-3.
4. the method according to claim 1 for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, feature
Be: the pH of water phase is adjusted to pH=2-3 with hydrochloric acid in step (2).
5. the method according to claim 1 for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, feature
Be: the amount of the ethyl acetate of extraction supernatant of bacteria solution is the 1/2 of supernatant of bacteria solution volume in step (2).
6. the method according to claim 1 for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, feature
Be: the amount of the ethyl acetate of aqueous phase extracted is the 1/2 of water phase volume in step (2).
7. the method according to claim 1 for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, feature
It is: step (2) are as follows: supernatant of bacteria solution is extracted twice with the ethyl acetate of its 1/2 volume, retains the water phase of lower layer；By water phase
PH be adjusted to pH=2-3 with hydrochloric acid, water phase is extracted twice with the ethyl acetate of its 1/2 volume again, collect upper layer it is organic
Phase.
8. the method according to claim 1 for isolating and purifying 7- hydroxy tropolone from bacterial supernatant, feature
Be: evaporated under reduced pressure described in step (3) and (4) be with Rotary Evaporators in 40-42 DEG C of water-bath evaporated under reduced pressure.
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| CA3083236A1 (en) * | 2017-12-05 | 2019-06-13 | Lonza Ltd | Methods of assaying tropolone |
| CN111893053B (en) * | 2019-10-15 | 2021-12-28 | 浙江大学 | Pseudomonas fluorescens 22G5 and application of active ingredient 7-hydroxy tropolone thereof in preventing and treating crop verticillium wilt |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3149608A1 (en) * | 1981-12-15 | 1983-08-04 | Dr. Madaus & Co, 5000 Köln | Substituted tropolones, process for the preparation thereof and pharmaceutical compositions containing these |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3149608A1 (en) * | 1981-12-15 | 1983-08-04 | Dr. Madaus & Co, 5000 Köln | Substituted tropolones, process for the preparation thereof and pharmaceutical compositions containing these |
Non-Patent Citations (4)
| Title |
|---|
| 7-Hydroxy Tropolone from Pseudomonas sp.[1];H. Korth等;《Zeitschrift Für Naturforschung C》;19811231;第36卷;第728-729页 * |
| Effect of Iron Limitation on"Pseudomonas plantarii"Growth and Tropolone and Protein Production;KOJI AZEGAMI等;《Applied & Environmental Microbiology》;19881231;第54卷(第3期);第844-847页 * |
| Production of tropoline by a pseudomonas;G.D.Lindberg等;《Journal of Natural Products》;20041231;第43卷(第5期);第592-594页 * |
| Tropolone as a Root Growth-Inhibitor Produced by a Plant Pathogenic Pseudomonas sp. Causing Seedling Blight of Rice;Koji Azegami等;《Ann.Phytopath.Soc.Japan》;19851231;第51卷;第315-317页 * |
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