CN106556694A - A kind of immune colloid gold detection card of carbendazim - Google Patents
A kind of immune colloid gold detection card of carbendazim Download PDFInfo
- Publication number
- CN106556694A CN106556694A CN201611097812.8A CN201611097812A CN106556694A CN 106556694 A CN106556694 A CN 106556694A CN 201611097812 A CN201611097812 A CN 201611097812A CN 106556694 A CN106556694 A CN 106556694A
- Authority
- CN
- China
- Prior art keywords
- detection
- carbendazim
- pvb
- sample pad
- nitrocellulose filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
The invention discloses a kind of immune colloid gold detection card of carbendazim, including detection card shell, the upper surface of the detection card shell is provided with well and detection window, the detection card inside the shell is provided with test strip, the test strip is thereon successively to paste sample pad by adhesive sticker by PVB offset plates, gold conjugation pad, nitrocellulose filter and adsorptive pads are constituted, the middle part of the PVB offset plates is stacked to be pasted with nitrocellulose filter, it is parallel on the nitrocellulose filter to be provided with detection line and nature controlling line, one end of the PVB offset plates is stacked to paste sample pad, and the stacked stickup adsorptive pads of the other end of PVB offset plates, one section of gold conjugation pad of sandwiched stickup between the sample pad and the clinch of nitrocellulose filter.The present invention is adapted in basic unit's popularization and application by the residual quantity of carbendazim in food can be fast and effectively detected with immunologic method, also has the advantages that low manufacture cost, simple to operate and accurately and reliably, with application value.
Description
Technical field
The present invention relates to cereal foods Pesticide Residues detection technique field, specially a kind of immune colloid gold of carbendazim
Detection card.
Background technology
Carbendazim also known as carbendazol, carbendazol, are a kind of broad-spectrum germicides, to various crop by funguses（Such as
Fungi Imperfecti, many ascomycetess）The disease for causing has prevention effect.At present, carbendazim has been widely used in wheat and barley disease, Oryza sativa L. disease
In the preventing and treating of evil, cotton disease, Brassica campestris L disease, vegetable disease, fruit diseases and floral crops diseases.Can be used for foliar spray, seed
Process and soil treatment etc..As carbendazim has low toxicity to people, animal, Fish and Apiss etc., accordingly, it would be desirable to remaining in food
Carbendazim is detected.European Union specifies MRL of the carbendazim in food（MRL）For 0.1mg/kg.
It is fixed carbendazim residue detection mainly to be carried out with high performance liquid chromatography and LC-MS etc. in prior art
Property and quantitative analysis.But, these methods are had high demands to experimental instrument and equipment, cumbersome, and the specialty of operator is known
Knowledge has high demands, it is difficult to promote on a large scale.Therefore, how to design a kind of simple to operate, accurately and reliably and quickly and easily detection food
In product, the immune colloid gold detection card of carbendazim residual is very important.
The content of the invention
It is an object of the invention to provide a kind of immune colloid gold detection card of carbendazim, by with immunologic method
The residual quantity of carbendazim in food can be fast and effectively detected, is adapted in basic unit's popularization and application, also with low manufacture cost, behaviour
Make it is simple and accurately and reliably the advantages of, solve the problems, such as proposed in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme：A kind of immune colloid gold detection card of carbendazim, bag
Detection card shell is included, the upper surface of the detection card shell is provided with well and detection window, and the detection card inside the shell is provided with
Test strip, the test strip are thereon successively to paste sample pad, gold conjugation pad, nitre by adhesive sticker by PVB offset plates
Acid cellulose film and adsorptive pads are constituted, and the middle part of the PVB offset plates is stacked to be pasted with nitrocellulose filter, the cellulose nitrate
Parallel on plain film to be provided with detection line and nature controlling line, one end of the PVB offset plates is stacked to paste sample pad, and PVB offset plates is another
Stickup adsorptive pads are put in endlap, and between the sample pad and the clinch of nitrocellulose filter, one section of gold colloidal of sandwiched stickup is combined
Pad.
Preferably, the nitrocellulose filter is just to detection window.
Preferably, have at regular intervals between the detection line and nature controlling line.
Preferably, the inner of the inner of the sample pad and adsorptive pads is each mutually overlapped with nitrocellulose filter respectively.
Preferably, the sample pad is made by heat absorbing glass fibrous membrane.
Compared with prior art, beneficial effects of the present invention are as follows：
Residual quantity by carbendazim in food can be fast and effectively detected with immunologic method of the invention, is adapted in base
Layer popularization and application, also have the advantages that low manufacture cost, simple to operate and accurately and reliably, with application value.
Description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the cross section structure diagram of test strips in the present invention.
In figure：1- detects card shell, 2- wells, 3- detection windows, 4- test strip, 5-PVB offset plates, 6- sample pad, 7-
Gold conjugation pad, 8- nitrocellulose filters, 9- adsorptive pads, 10- detection lines, 11- nature controlling lines.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1, Fig. 2 are referred to, the present invention provides a kind of immune colloid gold detection card technique scheme of carbendazim：A kind of many bacterium
The immune colloid gold detection card of spirit, including detection card shell 1, the upper surface of the detection card shell 1 is provided with well 2 and detection
Window 3, the detection window 3 are easy to testing staff to check testing result, are provided with test strip 4, the survey in the detection card shell 1
Strip 4 is thereon successively to paste sample pad 6, gold conjugation pad 7, nitrocellulose filter by adhesive sticker by PVB offset plates 5
8 and adsorptive pads 9 constituted, the middle part of the PVB offset plates 5 is stacked to be pasted with nitrocellulose filter 8, the nitrocellulose filter 8
It is above parallel to be provided with detection line 10 and nature controlling line 11, have at regular intervals between the detection line 10 and nature controlling line 11, the nitric acid
Just to detection window 3, one end of the PVB offset plates 5 is stacked to paste sample pad 6, and another endlap of PVB offset plates 5 to cellulose membrane 8
Stickup adsorptive pads 9 are put, the inner of the sample pad 6 and the inner of adsorptive pads 9 are each mutually overlapped with nitrocellulose filter 8 respectively,
Between the clinch of the sample pad 6 and nitrocellulose filter 8 it is sandwiched stickup one section of gold conjugation pad 7, the sample pad 6 by
Heat absorbing glass fibrous membrane is made.
Operation principle：First by making food to be detected into sample liquid, then sample liquid to be detected is dripped from well 2
In the sample pad 6 of the immune colloid gold detection card of injection carbendazim, then, using siphonage principle, can drive to be detected
Resisting carbendazim colloid gold label thing contained by sample liquid and gold conjugation pad 7 is spread to nitrocellulose filter 8 together, most
Afterwards, bear with and just testing result can be observed by detection window 3 in eight minutes or so：If detection line 10 and nature controlling line 11 are not
Show Color, then show that the immune colloid gold detection card of the carbendazim has failed；If detection line 10 and nature controlling line 11 are simultaneously
Show brownish red trace, then it represents that testing result is feminine gender, so as to the carbendazim content for illustrating to be detected in sample is not exceeded；Such as
The not Show Color of fruit detection line 10, and nature controlling line 11 shows brownish red trace, then it represents that testing result is the positive, so as to illustrate quilt
Carbendazim content overproof in detection sample.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (5)
1. the immune colloid gold detection of a kind of carbendazim blocks, including detection card shell（1）, it is characterised in that：The detection card is outer
Shell（1）Upper surface be provided with well（2）And detection window（3）, the detection card shell（1）Test strip is provided with inside（4）, it is described
Test strip（4）It is by PVB offset plates（5）It is upper that sample pad is pasted thereon successively by adhesive sticker（6）, gold conjugation pad（7）, nitre
Acid cellulose film（8）And adsorptive pads（9）Constituted, the PVB offset plates（5）Middle part stacked be pasted with nitrocellulose filter（8）,
The nitrocellulose filter（8）It is above parallel to be provided with detection line（10）And nature controlling line（11）, the PVB offset plates（5）One end be stacked
Paste sample pad（6）, and PVB offset plates（5）The other end stacked paste adsorptive pads（9）, the sample pad（6）With celluloid
Film（8）Clinch between it is sandwiched stickup one section of gold conjugation pad（7）.
2. the immune colloid gold detection of a kind of carbendazim according to claim 1 blocks, it is characterised in that：The cellulose nitrate
Plain film（8）Just to detection window（3）.
3. the immune colloid gold detection of a kind of carbendazim according to claim 1 blocks, it is characterised in that：The detection line
（10）And nature controlling line（11）Between have at regular intervals.
4. the immune colloid gold detection of a kind of carbendazim according to claim 1 blocks, it is characterised in that：The sample pad
（6）Inner and adsorptive pads（9）The inner each respectively with nitrocellulose filter（8）Mutually overlap.
5. the immune colloid gold detection of a kind of carbendazim according to claim 1 blocks, it is characterised in that：The sample pad
（6）It is made by heat absorbing glass fibrous membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611097812.8A CN106556694A (en) | 2016-12-03 | 2016-12-03 | A kind of immune colloid gold detection card of carbendazim |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611097812.8A CN106556694A (en) | 2016-12-03 | 2016-12-03 | A kind of immune colloid gold detection card of carbendazim |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106556694A true CN106556694A (en) | 2017-04-05 |
Family
ID=58445690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611097812.8A Pending CN106556694A (en) | 2016-12-03 | 2016-12-03 | A kind of immune colloid gold detection card of carbendazim |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106556694A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203037655U (en) * | 2012-07-27 | 2013-07-03 | 长沙安迪生物科技有限公司 | Rapid detection card for carbendazim |
| CN105424939A (en) * | 2015-12-23 | 2016-03-23 | 中国烟草总公司郑州烟草研究院 | Test paper strip for detecting carbendazim, preparation method and application thereof |
| CN105572373A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card |
-
2016
- 2016-12-03 CN CN201611097812.8A patent/CN106556694A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203037655U (en) * | 2012-07-27 | 2013-07-03 | 长沙安迪生物科技有限公司 | Rapid detection card for carbendazim |
| CN105572373A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card |
| CN105424939A (en) * | 2015-12-23 | 2016-03-23 | 中国烟草总公司郑州烟草研究院 | Test paper strip for detecting carbendazim, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lu et al. | Nondestructive evaluation of quality changes and the optimum time for harvesting during jujube (Zizyphus jujuba Mill. cv. Changhong) fruits development | |
| CN104251904B (en) | A kind of test paper of Quantitative detection pyrethrin pesticide | |
| CN102798719B (en) | Test paper strip for rapidly detecting traces of chlorothalonil and preparation method thereof | |
| Cole et al. | Dermatitis in Ecuadorean farm workers | |
| Dövényi-Nagy et al. | Pre-harvest modelling and mitigation of aflatoxins in maize in a changing climatic environment—A review | |
| CN108535249A (en) | Fast Determination of Pesticide Residue point card | |
| CN105044101A (en) | Quick detection card for pesticide residues based on naked eye visual colorimetric determination | |
| DE202015100836U1 (en) | Multifunctional urine pad for pets | |
| CN106556694A (en) | A kind of immune colloid gold detection card of carbendazim | |
| CN102507931A (en) | Colloidal gold immunochromatographic assay test strip for pyrethroid pesticides and preparation and use methods | |
| CN204719028U (en) | Detect the synchronous test card of five kinds of aminoglycoside antibioticss | |
| CN2833595Y (en) | Colloid gold test paper for quick detection of metalaxyl residue | |
| CN105004863A (en) | Colloidal gold immunochromatography test paper for rapidly detecting soybean sensitizing protein beta-conglycinin, and production method thereof | |
| Lu et al. | Research on biomechanical properties of laver (Porphyra yezoensis Ueda) for mechanical harvesting and postharvest transportation | |
| WO2010078868A1 (en) | Method and device for measuring the activity of enzymes after inhibitor removal | |
| CN110095580A (en) | A kind of food pesticide residue detection device | |
| CN202794181U (en) | Test paper card for rapid detection of aflatoxin B1 residue | |
| Kadam et al. | Mechanical characterization of brown and green coconut husk | |
| DE102020100180B4 (en) | Immunochemical device and lateral flow immunoassay method for the determination of pharmaceutical residues and impurities in breast milk | |
| An et al. | Exposure risks to pesticide applicators posed by the use of electric backpack sprayers and stretcher-mounted sprayers in orchards | |
| CN204496549U (en) | A kind of crop pest identification and condition assessment device | |
| CN205015344U (en) | Short -term test soybean sensitization albumen glycinin's colloidal gold immunoassay chromatography test paper | |
| CN103439504B (en) | Immune chromatography test paper of quick detection fleraxacin and preparation method thereof | |
| CN206594064U (en) | A kind of residues of pesticides chemical fast determining method detects instrumentation | |
| CN109342721A (en) | Water suction test paper and preparation method thereof for immunoreagent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170405 |
|
| RJ01 | Rejection of invention patent application after publication |