CN106554987A - The test kit and its detection method of antibiotic in a kind of detection food-borne animal tissue - Google Patents
The test kit and its detection method of antibiotic in a kind of detection food-borne animal tissue Download PDFInfo
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- CN106554987A CN106554987A CN201510638698.4A CN201510638698A CN106554987A CN 106554987 A CN106554987 A CN 106554987A CN 201510638698 A CN201510638698 A CN 201510638698A CN 106554987 A CN106554987 A CN 106554987A
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Abstract
The invention discloses a kind of test kit and its detection method for detecting antibiotic in food-borne animal tissue, belongs to field of detection of food safety.Detection kit is constituted by detecting main body, xx and xx.Detection main body is homodisperse detection thalline, acid-base indicator and the solid medium for thalli growth desired nutritional material.Detection kit is divided into two kinds of forms of 96 hole detection plates or single detection pipe.Using the test kit of the present invention, operator need to only be directed to the tissue sample of pretest when in use and carry out once simple pretreatment, it is incubated the detection that 3h is just capable of achieving the antibiotic general status to remaining in animal tissue again within specified temperatures, detectable multiclass antibiotic, up to more than tens kinds of detection project is minimum up to 1-2ppb to the detection limit of part antibiotic.Therefore, the present invention can realize carrying out the general status of antibiotic remainss in food-borne animal tissue the higher half-quantitative detection of simple, quick, accuracy.
Description
Technical field
The present invention relates to a kind of test kit and its detection method for detecting antibiotic in food-borne animal tissue, category
In field of detection of food safety.
Background technology
Antibiotic is most common and most important polluter in animal food safety.People's intake for a long time is containing antibiosis
After the food of element, constantly in people's body accumulation, after accumulating to a certain extent, toxicity will be produced to human body
Effect, causes all many-sided adverse effects such as anaphylaxiss, teratogenesis, mutagenesis and the hormone-like effect of people.
In recent years, the safety of animal food has become the problem of social common concern.
At present, be used for both at home and abroad detecting the method for drug residue have microbial method, immunoassay, gas phase,
Liquid chromatography, high performance liquid chromatography (HPLC), liquid chromatography mass combination method (LC-MS) etc..Also have very
Multiplex biochemical reagents box is detecting antibiotic remainss.HPLC method pre-treatments are more complicated, required instrument also costly,
But the features such as having efficient, sensitive, accurate, is that such drug residue detects most common method；LC-MS methods
Analyst coverage is wide, is not necessary to derivatization step but required instrument requirements are high, expensive to be used for residual confirmation；
Immunoassay specificity is stronger than microbial method, but due to there is certain cross reaction, is only applicable to quick sieve
Choosing.Nutrition code committee for the definition of rapid screening method, i.e.,：(1) these methods can be detected most
The sample concentration of high residue limitation concentration level；(2) detection of batch samples can be carried out, it is quickly, sensitive
Property it is high；(3) it is convenient to operation in a non-laboratory environment.Compared with additive method, microbial method has
The advantages of sample pre-treatments are simple and convenient to operate, possess certain sensitivity and specificity, can be used as a kind of sieve
Choosing method is detected simultaneously to gross sample.
It is many in the method for antibiotic remainss in microorganism detection animal tissue at present, such as German three dish method, Europe
Four dish method of alliance, seven dish methods, STOP methods, FAST methods, CAST methods etc..But it is by inhibition zone size
Observation carry out interpretation so that the judgement to result is not bright and clear enough, causes detection limit higher, and exists artificial
Error caused by factor.The present invention suppresses method based on growth of microorganism, by the suppression situation and face of thalli growth
The change of color is to combination, so as to indirectly reflect the situation of antibiotic remainss in animal tissue and so that right
As a result interpretation is more bright and clear；The heat-preserving equipment of two kind different temperatures is used in detection process only, without the need for specific
More accurate instrument；It is easy to operate without the need for it is professional operation and sensitivity is higher, minimum detectability is reachable
1-2ppb.Therefore the present invention can realize carrying out letter to the residual condition of antibiotic in food-borne animal tissue
Just, fast, efficiently, accurately detect.
The content of the invention
Present invention aims to the deficiencies in the prior art, there is provided a kind of easy, quick, efficient, accurate
The residual condition of antibiotic in true detection food-borne animal tissue.
The detection kit of antibiotic in a kind of food-borne animal tissue, is made up of detection main body, xx and xx.
Detection main body is homodisperse detection thalline, acid-base indicator and consolidating for thalli growth desired nutritional material
Body culture medium.Detection kit is divided into two kinds of forms of 96 hole detection plates or single detection pipe.
The detection thalline is bacstearothermophilus suspension；
The acid-base indicator is bromocresol purple；
Further, the preparation process of spore bacteria suspension can be divided into Plating, concentration Plating and shake bacterium method,
Comprise the following steps that：
1. Plating：
1) colony inoculation is taken to containing sterile liquid training from the bacterium colony group of the bacstearothermophilus for having activated
In the test tube of foster base, it is placed under 56-65 DEG C of environment, shaking bacterium 24h or so；
2) next day bacterium solution is spread evenly across in the culture dish containing solid medium with certain inoculum concentration, be placed in
2-3d is cultivated under 56-65 DEG C of environment；
3) plate is placed in into 37 DEG C or 70 DEG C and cultivates 3-4d again；
4) thalline is eluted from culture medium with PBS, is placed in centrifuge tube and is placed in 80-85 DEG C
Under the conditions of 15-20min obtaining purer spore bacteria suspension.
2. concentration Plating：
1) take a colony inoculation from the bacterium colony group of the bacstearothermophilus for having activated to train to 200ml liquid
Bacterium 24h or so is shaken in foster base and under 56-65 DEG C of environment；
2) next day by bacterium solution centrifugation, with PBS by bacterium solution concentration be original volume 1/20-1/5；
3) again the bacterium solution coating of concentration is seeded on the plate containing solid medium, is placed in 56-65 DEG C of ring
1d or so is cultivated under border；
4) thalline is eluted from culture medium with PBS, is placed in centrifuge tube and is placed in 80-85 DEG C of bar
Under part, 15-20min is obtaining purer spore bacteria suspension.
3. bacterium method is shaken：
1) take a colony inoculation from the bacterium colony group of the bacstearothermophilus for having activated to train to 200ml liquid
Bacterium 24h or so is shaken in foster base and under 56-65 DEG C of environment；
2) bacterium solution is centrifuged, with PBS by the 1/20-1/5 that bacterium solution concentration is original volume, and in 64 DEG C
Culture 24-36h；
3) next day 15-20min under the conditions of 80-85 DEG C (is noted with obtaining purer spore bacteria suspension：This method
Operation it is relatively simple, but spore output capacity two methods are low earlier above).
Further, the component for preparing the solid medium of spore bacteria suspension is peptone 8%-12%, yeast extract
2%-5%, Sodium Chloride 3%-10%, agar 10%-17%, ultrapure appropriate amount of water；Fluid medium consists of albumen
Peptone 8%-11%, yeast extract 1%-8%, Sodium Chloride 2%-10%, ultrapure appropriate amount of water.
Further, the preparation of main body is detected, is comprised the steps：The detection culture medium for preparing is existed
High temperature sterilize 30min under the conditions of 110-120 DEG C, when culture medium temperature is down to 60-70 DEG C, adds a certain amount of
Spore bacteria suspension cause spore total concentration in the medium in 6*105-4*106In the range of CFU/ml,
During culture medium to be added after even mixing the ELISA Plate of 96 orifice plates, sealed with heat-sealing aluminium film, be placed in 4-8 DEG C
Deposit under environment；
Further, detect that nutrient media components is：：Peptone 5%-15%, glucose 2%-10%, Sodium Chloride 2%-10%,
Agar 2%-15%, sodium carboxymethyl cellulose 2%-10%, guar gum 2%-10%, bromocresol purple 1%-5%, ethanol
Solution 0.1%-1%.
Using antibiotic in mentioned reagent box detection food-borne animal tissue, comprise the following steps that：
The first step, by animal tissue's homogenizing to be checked, taking a certain amount of homogeneous structure carries out quick-freezing, then by group
Rapid defrosting (making tissue fluid discharge from cell) is knitted, is centrifuged under the speed higher than 10000r/min
10-15min。
Second step, takes during a certain amount of supernatant adds to detection micropore or detection pipe and seals, be placed in 64 DEG C
3h or so is incubated under environment.If in test serum, certain antibiotic content is more than detection limit, thalli growth
It is suppressed, so that result keeps blue, bluish violet or taupe；If conversely, not containing in test serum
Antibiotic or content below detection limit, then growth of microorganism produce acid so that testing result present yellow or
Yellow green.
The present invention relates in food-borne animal tissue antibiotic overall content detection.Using the reagent of the present invention
Box, operator carry out multiple pretreatment without the need for the tissue sample to required detection when in use, only need to be for pre-
The tissue sample of test carries out once simple pretreatment, then be incubated within specified temperatures 3h be just capable of achieving it is right
The detection of the antibiotic general status remained in animal tissue, can detect multiclass antibiotic, and detection project is up to
It is more than tens kinds, minimum up to 1-2ppb to the detection limit of part antibiotic.Therefore, the present invention can be realized
Higher half of simple, quick, accuracy is carried out to the general status of antibiotic remainss in food-borne animal tissue
Detection by quantitative.
It is an advantage of the current invention that the suppression situation of thalli growth is combined with the change of color so that right
As a result interpretation is more bright and clear, and improves detection sensitivity；Two kind different temperatures are used in detection process only
Heat-preserving equipment, without the need for specific more accurate instrument；It is easy to operate without the need for professional operation.
Specific embodiment
First, detect the preparation of the test kit of antibiotic in food-borne animal tissue
The preparation process of spore bacteria suspension can be divided into Plating, concentration Plating and shake bacterium method, concrete steps
It is as follows：
1. Plating：
1) colony inoculation is taken to containing sterile liquid training from the bacterium colony group of the bacstearothermophilus for having activated
In the test tube of foster base, it is placed under 56-65 DEG C of environment, shaking bacterium 24h or so；
2) next day bacterium solution is spread evenly across in the culture dish containing solid medium with certain inoculum concentration, be placed in
2-3d is cultivated under 56-65 DEG C of environment；
3) plate is placed in into 37 DEG C or 70 DEG C and cultivates 3-4d again；
4) thalline is eluted from culture medium with PBS, is placed in centrifuge tube and is placed in 80-85 DEG C
Under the conditions of 15-20min obtaining purer spore bacteria suspension.
2. concentration Plating：
1) take a colony inoculation from the bacterium colony group of the bacstearothermophilus for having activated to train to 200ml liquid
Bacterium 24h or so is shaken in foster base and under 56-65 DEG C of environment；
2) next day by bacterium solution centrifugation, with PBS by bacterium solution concentration be original volume 1/20-1/5；
3) again the bacterium solution coating of concentration is seeded on the plate containing solid medium, is placed in 56-65 DEG C of ring
1d or so is cultivated under border；
4) thalline is eluted from culture medium with PBS, is placed in centrifuge tube and is placed in 80-85 DEG C of bar
Under part, 15-20min is obtaining purer spore bacteria suspension.
3. bacterium method is shaken：
1) take a colony inoculation from the bacterium colony group of the bacstearothermophilus for having activated to train to 200ml liquid
Bacterium 24h or so is shaken in foster base and under 56-65 DEG C of environment；
2) bacterium solution is centrifuged, with PBS by the 1/20-1/5 that bacterium solution concentration is original volume, and in 64 DEG C
Culture 24-36h；
3) next day 15-20min under the conditions of 80-85 DEG C (is noted with obtaining purer spore bacteria suspension：This method
Operation it is relatively simple, but spore output capacity two methods are low earlier above).
Further, the component for preparing the solid medium of spore bacteria suspension is peptone 8%-12%, yeast extract
2%-5%, Sodium Chloride 3%-10%, agar 10%-17%, ultrapure appropriate amount of water；Fluid medium consists of albumen
Peptone 8%-11%, yeast extract 1%-8%, Sodium Chloride 2%-10%, ultrapure appropriate amount of water.
Further, the preparation of main body is detected, is comprised the following steps that：The preparation of detection main body comprises the steps：
By the detection culture medium for preparing under the conditions of 110-120 DEG C high temperature sterilize 30min, treat that culture medium temperature is down to
When 60-70 DEG C, add a certain amount of spore bacteria suspension that spore total concentration in the medium is existed
6*105-4*106In the range of CFU/ml, culture medium is added in the ELISA Plate of 96 orifice plates after uniform mixing, used
Heat-sealing aluminium film is sealed, and is placed in depositing under 4-8 DEG C of environment.
Further, detect that nutrient media components is：：Peptone 5%-15%, glucose 2%-10%, Sodium Chloride 2%-10%,
Agar 2%-15%, sodium carboxymethyl cellulose 2%-10%, guar gum 2%-10%, bromocresol purple 1%-5%, ethanol
Solution 0.1%-1%.
2nd, sample detection
1. take animal tissue to be checked appropriate, homogenizing will be first organized with homogenizer；
2. by the tissue quick-freezing of homogenizing, then which is thawed under conditions of less than 30 DEG C rapidly, to be not less than
The centrifugation 10-15min of 10000r/min；
3. the detection micropore or detection pipe of respective numbers are taken (if detection micropore according to the quantity of tissue to be detected
The aluminium film destroyed when micropore is taken on other micropores need to be avoided)；
4. take the umbrella organisations' liquid after centrifugation appropriate (depending on Specific amounts is by the form of product is detected) and add to detection product
In, sealed with glued membrane and carry out labelling；
5. the detection micropore or detection pipe of good seal are placed under 64+0.5 DEG C of environment and are incubated, such as water-bath or incubator.
Can observe between incubation 3-3.5h, if detection hole is changed into yellow or yellow green, testing result is judged to negative；
If detection hole keeps purple, bluish violet or in taupe, testing result is judged to the positive.
3rd, lowest detection line is tested (by taking benzylpenicillin as an example)
Take benzylpenicillin dry powder 1mg；The mother solution of 1mg/ml is made into the dissolving of 1ml pure water；It is this is female with pure water
Liquid dilutes 1000 times of benzylpenicillin solution for being configured to 1ug/ml；Take from the benzylpenicillin solution of 1ug/ml
1ul is added in 1ml animal tissues extracting solution, and the content for making benzylpenicillin in tissue fluid is 1ng/ml；According to
The detecting step of test kit is detected that its testing result is the positive.
Claims (5)
1. a kind of test kit for detecting antibiotic in food-borne animal tissue, it is characterised in that test kit is by examining
Survey main body and colorimetric card composition；Detection main body is for homodisperse detection thalline, acid-base indicator and supplies thalline
The solid medium of growth desired nutritional material.
2. the test kit for detecting antibiotic in food-borne animal tissue according to claim 1, its feature
It is that the test kit is divided into two kinds of forms of 96 hole detection plates or single detection pipe.
3. the test kit for detecting antibiotic in food-borne animal tissue according to claim 1, its feature
It is that the detection thalline is bacstearothermophilus suspension.
4. the test kit for detecting antibiotic in food-borne animal tissue according to right 1, it is characterised in that
The preparation of detection main body comprises the steps：By the detection culture medium for preparing under the conditions of 110-120 DEG C high temperature
Sterilizing 30min, when culture medium temperature is down to 60-70 DEG C, adds a certain amount of spore bacteria suspension to cause spore
Total concentration in the medium is in 6*105-4*106In the range of CFU/ml, culture medium is added after uniform mixing
In the ELISA Plate of 96 orifice plates, sealed with heat-sealing aluminium film, be placed in depositing under 4-8 DEG C of environment；
Wherein detect that nutrient media components is：Peptone 5%-15%, glucose 2%-10%, Sodium Chloride 2%-10%, agar
2%-15%, sodium carboxymethyl cellulose 2%-10%, guar gum 2%-10%, bromocresol purple 1%-5%, ethanol solution
0.1%-1%.
5. a kind of method that usage right requires antibiotic in the test kit detection food-borne animal tissue described in 1, tool
Body step includes：
The first step, by animal tissue's homogenizing to be checked, taking a certain amount of homogeneous structure carries out quick-freezing, then will organize fast
Speed is thawed (tissue fluid is discharged from cell), is centrifuged under the speed higher than 10000r/min
10-15min；
Second step, takes during a certain amount of supernatant adds to detection micropore or detection pipe and seals, be placed in 64 DEG C of environment
Lower incubation 3h or so.If more than detection limit, thalli growth is subject to certain antibiotic content in test serum
Suppress, so that result keeps blue, bluish violet or taupe；If conversely, antibiosis is not contained in test serum
Below detection limit, then growth of microorganism produces acid for element or content so that the yellow or yellowish green that testing result is presented
Color.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107314980A (en) * | 2017-06-08 | 2017-11-03 | 中国肉类食品综合研究中心 | Multiple antibiotic residues detection kit and detection method in a kind of animal derived food |
| CN112063683A (en) * | 2020-11-16 | 2020-12-11 | 广州智汇生物科技有限公司 | Method and kit for detecting antibiotic residues in food and production and circulation processes thereof |
| CN113249431A (en) * | 2021-05-13 | 2021-08-13 | 吉林农业大学 | Test piece for penicillin G residue in milk, preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0755456B1 (en) * | 1995-02-01 | 2000-11-08 | Dsm N.V. | A rapid microbiological test for the detection of antibacterial compounds |
| WO2001025795A2 (en) * | 1999-10-04 | 2001-04-12 | Dsm N.V. | One step test to detect antimicrobial residues in eggs |
| CN1816633A (en) * | 2003-07-02 | 2006-08-09 | 帝斯曼知识产权资产管理有限公司 | Improved test system for the determination of the presence of an antibiotic in a fluid |
| CN101131362A (en) * | 2007-09-29 | 2008-02-27 | 中国农业科学院上海兽医研究所 | Method for fast filtering antibacterial medicine residue in milk-like liquid sample |
| CN101633948A (en) * | 2009-08-20 | 2010-01-27 | 华南理工大学 | Kit test method of beta-lactam antibiotics residue in milk |
| CN201581078U (en) * | 2009-08-27 | 2010-09-15 | 北京智云达科技有限公司 | Detecting plate |
| KR20140073879A (en) * | 2012-12-07 | 2014-06-17 | (주)나비바이오텍 | Qualitative antibiotics residue detection media |
-
2015
- 2015-09-29 CN CN201510638698.4A patent/CN106554987A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0755456B1 (en) * | 1995-02-01 | 2000-11-08 | Dsm N.V. | A rapid microbiological test for the detection of antibacterial compounds |
| WO2001025795A2 (en) * | 1999-10-04 | 2001-04-12 | Dsm N.V. | One step test to detect antimicrobial residues in eggs |
| CN1816633A (en) * | 2003-07-02 | 2006-08-09 | 帝斯曼知识产权资产管理有限公司 | Improved test system for the determination of the presence of an antibiotic in a fluid |
| CN101131362A (en) * | 2007-09-29 | 2008-02-27 | 中国农业科学院上海兽医研究所 | Method for fast filtering antibacterial medicine residue in milk-like liquid sample |
| CN101633948A (en) * | 2009-08-20 | 2010-01-27 | 华南理工大学 | Kit test method of beta-lactam antibiotics residue in milk |
| CN201581078U (en) * | 2009-08-27 | 2010-09-15 | 北京智云达科技有限公司 | Detecting plate |
| KR20140073879A (en) * | 2012-12-07 | 2014-06-17 | (주)나비바이오텍 | Qualitative antibiotics residue detection media |
Non-Patent Citations (7)
| Title |
|---|
| EKENE VIVIENNE EZENDUKA等: "Rapid detection of antimicrobial residues in poultry: A consequence of non-prudent use of antimicrobials", 《HEALTH》 * |
| H. CANTWELL等: "Evaluation of the Premi Test and comparison with the One-Plate Test", 《FOOD ADDITIVES & CONTAMINANTS》 * |
| KILINC BERNA等: "comparison of the European economic community four-olate test，premi test and enzyme-linked immunosorbent assay for oxytetracycline residue detection in rainbow trout", 《JOURNAL OF MUSCLE FOODS》 * |
| MARILYN J SCHNEIDER等: "A comparison of the FAST, Premi and KIS tests for screening antibiotic residues in beef kidney juice and serum", 《ANAL BIOANAL CHEM》 * |
| 何礼贤等: "《临床微生物与感染》", 31 July 2010, 中国医药科技出版社 * |
| 罗红霞等: "《乳制品加工技术》", 31 January 2015, 中国轻工业出版社 * |
| 胡昭庚等: "《食用菌制种技术》", 31 January 1999, 北京农业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107314980A (en) * | 2017-06-08 | 2017-11-03 | 中国肉类食品综合研究中心 | Multiple antibiotic residues detection kit and detection method in a kind of animal derived food |
| CN107314980B (en) * | 2017-06-08 | 2020-02-18 | 中国肉类食品综合研究中心 | Detection kit and detection method for multiple antibiotic residues in animal-derived food |
| CN112063683A (en) * | 2020-11-16 | 2020-12-11 | 广州智汇生物科技有限公司 | Method and kit for detecting antibiotic residues in food and production and circulation processes thereof |
| CN112063683B (en) * | 2020-11-16 | 2021-02-12 | 广州智汇生物科技有限公司 | Method and kit for detecting antibiotic residues in food and production and circulation processes thereof |
| CN113249431A (en) * | 2021-05-13 | 2021-08-13 | 吉林农业大学 | Test piece for penicillin G residue in milk, preparation method and application thereof |
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