CN106526189A - Lincomycin detection kit - Google Patents
Lincomycin detection kit Download PDFInfo
- Publication number
- CN106526189A CN106526189A CN201610983619.8A CN201610983619A CN106526189A CN 106526189 A CN106526189 A CN 106526189A CN 201610983619 A CN201610983619 A CN 201610983619A CN 106526189 A CN106526189 A CN 106526189A
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- China
- Prior art keywords
- lincomycin
- absorption pad
- reagent
- hapten
- film
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- Cell Biology (AREA)
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- Food Science & Technology (AREA)
- Biotechnology (AREA)
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- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a lincomycin detection kit. The lincomycin detection kit comprises test paper and a micro-porous reagent. The test paper comprises a reaction film, a sample absorption pad, a water absorption pad, a protection film and a bottom board and is formed by adhering the ample absorption pad, the reaction film, the water absorption pad and the protection film on the bottom borad sequentially, wherein the reaction film is provided with a detection area and a quality control area, a lincomycin hapten-carrier protein conjugate wraps the detection area, and an antiantibody wraps the quality control area. Lincomycin specificity antibody-colloidal gold marker is freeze-dried on the micro-porous reagent, and the micro-porous reagent is provided with micro-porous plugs. The lincomycin detection kit has the advantages of wide linear dynamics range, long optical signal duration time, simplicity and rapidity in analysis method, stable results, small errors, high safety and long service life. Through chemiluminescence immunoassay, the lincomycin detection kit succeeds in detecting substances which cannot be detected by the methods such as radioimmunoassay and enzyme-linked immunoassay, thereby being of great significance to early clinical diagnosis.
Description
Technical field
The present invention relates to a kind of test kit, specifically a kind of lincomycin assay kit.
Background technology
In detection Lac Bovis seu Bubali, the technology of lincomycin antibiotic remainss mainly has following several method at present：（1）Microorganism is received
Resistance detection method, tests (micmorganisminhibitorytest, abbreviation MIT) also known as Antimicrobial, according to antibiotic to spy
The physiological function of different microorganism, the inhibitory action of breeding metabolism are qualitatively or quantitatively determining antimicrobial agents residual in sample
Amount, is that antibiotic is more classical and using wide method in detection breast.Antibiotic remainss touchstone in China's sweet milk
（GB4789.27-2003）Detection method, the 1970s and 1980s external inhibition zone test for generally adopting, also turbidity test, mesh
Front color changing type detection kit on the market belongs to such.Variety classes indicator bacteria is to variety classes antibiotic sensitive degree
Difference, selects appropriate detection indicator bacteria reduce test limit, improves detection sensitivity.But, microorganism detection operating cost
When it is laborious, be not suitable for doing large-scale production.（2）It is after the nineties 20th century of Physico-chemical tests method, most to determine in food
The Physico-chemical tests method of lincomycin class drug residue relies primarily on liquid chromatography technology and is separated, next to that color/mass spectrometry
The detection methods such as technology, gas chromatography, high performance thin-layer chromatography, because of its respective distinctive performance, in antibiotics leftover detection side
Slightly apply in face.Drug residue in chromatography detection Lac Bovis seu Bubali will through sample treatment (include the extraction of sample, deproteinization, from
The heart, chromatography column purification, the step such as derivatization), the separation of left drug and the detection of left drug.Physico-chemical tests method utilizes antibiosis
Specific response that group in plain molecule has or property can carry out qualitative and quantitative analysis and medicine mirror determining its content
It is fixed, can be used as the confirmation method of antibiotic detection in breast.The method detection sensitivity is higher, but instrument and testing cost height, detection
Program is complicated, time-consuming etc., and these unfavorable factors make the method be only limitted to laboratory milk sample measure.（3）Immune analysis method is exempted from
Epidemiology analytic process is the analysis method based on the specificity of antigen and antibody, reversibility association reaction, current drug residue
Immuno analytical method mainly divides three major types：Relatively independent analysis method, immuno analytical method and conventional physical and chemical analysis technology connection
Method, immunity receptor method.Holland vanweeman, schurrs in 1973 and Sweden Engvall, Perlmann are proposed respectively
Enzyme linked immunosorbent assay, over more than 30 years, Chinese scholars have carried out numerous studies to antibiotic in immunological method detection food.
But as antibiotic is hapten, antigen constructing technology difficulty is big, immunologic opsonin is strong, testing cost is high, current IDEXX experiments
The researchs of the company in terms of analytic process is exempted from such as room company, Cha coral Sciences are more ripe, and the domestic technology is sent out still in research
Zhan Zhong.Be currently mostly with elisa, radioimmunoassay luminescence method, fluorescent immune method, chemoluminescence method etc., than
Enzyme knowable to more several immuno-labelling technique sensitivity is exempted from compared with luminous, and it is relatively low that its enzyme exempts from sensitivity.
The content of the invention
It is an object of the invention to provide a kind of lincomycin assay kit, to solve what is proposed in above-mentioned background technology
Problem.
For achieving the above object, the present invention provides following technical scheme：
A kind of lincomycin assay kit, including reagent paper and micropore reagent, the reagent paper include reaction film, sample absorption pad,
Adsorptive pads, protecting film, base plate, have detection zone and quality control region on the reaction film, detection zone is coated with lincomycin hapten-load
Body protein conjugate, quality control region are coated with anti antibody, and lyophilizing on the micropore reagent has lincomycin specific antibody-gold colloidal
Label.
As further scheme of the invention：The reagent paper by sample absorption pad, reaction film, adsorptive pads, protecting film successively
It is pasted onto on base plate and constitutes, there is on the micropore reagent micropore plug.
As further scheme of the invention：Protecting film is pasted with the sample absorption pad, is test side, is had above
MAX mark lines.
As further scheme of the invention：The detection zone is located at the one end for the protecting film for being bordering on MAX labellings, described
Quality control region is located remotely from one end of the protecting film of MAX labellings.
As further scheme of the invention：The lincomycin hapten-carrier protein conjugate is by lincomycin half
Antigen is obtained with carrier protein couplet, and the carrier protein can be bovine serum albumin, ovalbumin, hemocyanin, thyroid
Albumen, human serum albumin.
As further scheme of the invention：Woods in the lincomycin specific antibody-colloid gold label thing can
Mycin specific antibody is prepared using lincomycin hapten-carrier protein conjugate as immunogen, and the woods can be mould
Plain specific antibody can be lincomycin monoclonal antibody or lincomycin polyclonal antibody.
Compared with prior art, the invention has the beneficial effects as follows：Lincomycin assay kit of the present invention has wide line
Property dynamics range, optical signal persistent period length, analysis method is easy quick, result is stable, error is little, safety good and use
Phase is long, and chemiluminescence immune assay can detect the material that the methods such as radioimmunoassay, RIA and enzyme immunoassay cannot be detected,
Early diagnosiss tool clinically is of great significance.
Specific embodiment
Below the technical scheme in the embodiment of the present invention is clearly and completely described.
In the embodiment of the present invention, a kind of lincomycin assay kit, including reagent paper and micropore reagent, the reagent paper include
Reaction film, sample absorption pad, adsorptive pads, protecting film, base plate, have detection zone and quality control region, detection zone coating on the reaction film
There is lincomycin hapten-carrier protein conjugate, quality control region is coated with anti antibody, and lyophilizing on the micropore reagent has woods mould
Plain specific antibody-colloid gold label thing.The reagent paper is pasted onto successively by sample absorption pad, reaction film, adsorptive pads, protecting film
Constitute on base plate, there is on the micropore reagent micropore plug.Protecting film is pasted with the sample absorption pad, is test side, on
There are MAX mark lines in face.The detection zone is located at the one end for the protecting film for being bordering on MAX labellings, and the quality control region has been located remotely from
One end of the protecting film of MAX labellings.Lincomycin hapten-carrier protein couplet the thing is by lincomycin hapten and load
Body protein is coupled and obtains, and the carrier protein can be bovine serum albumin, ovalbumin, hemocyanin, thyroprotein, human blood
Pure albumen.Lincomycin specific antibody in the lincomycin specific antibody-colloid gold label thing is can be mould with woods
Plain hapten-carrier protein conjugate is prepared as immunogen, and the lincomycin specific antibody can be lincomycin
Monoclonal antibody or lincomycin polyclonal antibody.
Embodiment 1
First, reagent paper（Referred to as test strips）The reagent paper is made up of base plate, sample absorption pad, reaction film, adsorptive pads, protecting film；
The sample absorption pad, reaction film, adsorptive pads and protecting film are pasted onto on base plate successively in order, the end of sample absorption pad with
Reaction film is connected, and the end of reaction film is connected with adsorptive pads, and the top of sample absorption pad is alignd with the top of base plate, adsorptive pads
Align with the end of base plate end；The reagent paper is pasted with protecting film, and protecting film is covered on sample absorption pad, is test side,
MAX printed words are printed on above；There are detection zone and quality control region on the reaction film, in the band perpendicular with the length of the test strips
Shape, detection zone are located at the one end for the protecting film for being bordering on MAX labellings, and quality control region is located remotely from the one of the protecting film of MAX labellings
End, detection zone are coated with lincomycin hapten-carrier protein conjugate（The coupling of lincomycin hapten-ovalbumin
Thing）, quality control region is coated with sheep anti mouse anti antibody；The base plate is PVC base plates；The sample absorption pad is suction strainer paper；The suction
Water cushion is absorbent paper；The reaction film is nitrocellulose filter；The protecting film is PE material protecting film.2nd, micropore reagent, institute
Stating lyophilizing on micropore reagent has lincomycin monoclonal antibody-colloid gold label thing；The micropore reagent has micropore plug.Will be upper
State micropore reagent and test kit is assembled into reagent paper, preserve, 12 months effect duration in 2 ~ 8 DEG C of environment.
The preparation method of the test kit is mainly included the following steps that：1）Preparing lyophilizing has lincomycin monoclonal antibody-glue
The micropore reagent of body gold label；2）Prepare the detection zone with coating lincomycin hapten-carrier protein conjugate and bag
By the reaction film of the quality control region of sheep anti mouse anti antibody；3）By 2）The reaction film for preparing and sample absorption pad, adsorptive pads, protection
Film, base plate are assembled into reagent paper；4）By 1）With 3）The lyophilizing for preparing has the micro- of lincomycin monoclonal antibody-colloid gold label thing
Hole reagent and reagent paper are assembled into test kit.
Lincomycin assay kit of the present invention has wide linear kineticss scope, optical signal persistent period length, analysis
Method is easy quickly, result is stable, error is little, safety good and validity period is long, and chemiluminescence immune assay can detect radiation
Early diagnosiss clinically are had highly important meaning by the material that the method such as immunoassay and enzyme immunoassay cannot be detected
Justice.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of spirit or essential attributes without departing substantially from the present invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each
Embodiment only includes an independent technical scheme, and this narrating mode of description is only this area for clarity
Technical staff should using description as an entirety, the technical scheme in each embodiment can also Jing it is appropriately combined, form this
Art personnel may be appreciated other embodiment.
Claims (6)
1. a kind of lincomycin assay kit, it is characterised in that including reagent paper and micropore reagent, the reagent paper include reaction
Film, sample absorption pad, adsorptive pads, protecting film, base plate, have detection zone and quality control region on the reaction film, detection zone is coated with woods
Can mycin hapten-carrier protein conjugate, quality control region is coated with anti antibody, and lyophilizing on the micropore reagent has lincomycin special
Heterogenetic antibody-colloid gold label thing.
2. lincomycin assay kit according to claim 1, it is characterised in that the reagent paper by sample absorption pad,
Reaction film, adsorptive pads, protecting film are pasted onto on base plate successively and constitute, and have micropore plug on the micropore reagent.
3. lincomycin assay kit according to claim 1, it is characterised in that be pasted with the sample absorption pad
Protecting film, is test side, has MAX mark lines above.
4. lincomycin assay kit according to claim 1, it is characterised in that the detection zone is located at and has been bordering on
One end of the protecting film of MAX labellings, the quality control region are located remotely from one end of the protecting film of MAX labellings.
5. lincomycin assay kit according to claim 1, it is characterised in that the lincomycin hapten-load
Body protein conjugate is obtained by lincomycin hapten and carrier protein couplet, the carrier protein can for bovine serum albumin,
Ovalbumin, hemocyanin, thyroprotein, human serum albumin.
6. lincomycin assay kit according to claim 1, it is characterised in that the lincomycin specificity resists
Lincomycin specific antibody in body-colloid gold label thing is using lincomycin hapten-carrier protein conjugate as exempting from
Epidemic focus is prepared, and the lincomycin specific antibody can be lincomycin monoclonal antibody or lincomycin Anti-TNF-α
Body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610983619.8A CN106526189A (en) | 2016-11-09 | 2016-11-09 | Lincomycin detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610983619.8A CN106526189A (en) | 2016-11-09 | 2016-11-09 | Lincomycin detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106526189A true CN106526189A (en) | 2017-03-22 |
Family
ID=58350906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610983619.8A Pending CN106526189A (en) | 2016-11-09 | 2016-11-09 | Lincomycin detection kit |
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| Country | Link |
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| CN (1) | CN106526189A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107037216A (en) * | 2017-04-17 | 2017-08-11 | 新疆维吾尔自治区疾病预防控制中心 | The method of lincomycin in immunochromatographic measurement milk is quenched based on background fluorescence |
| CN108165532A (en) * | 2017-12-27 | 2018-06-15 | 江南大学 | One plant of woods can amine versatility monoclonal antibody hybridoma cell strain and its application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202710567U (en) * | 2012-06-15 | 2013-01-30 | 北京中检葆泰生物技术有限公司 | Colloidal gold test paper strip and kit both capable of rapidly detecting lincomycin |
| CN203069594U (en) * | 2012-10-22 | 2013-07-17 | 北京勤邦生物技术有限公司 | Colloidal gold test strip for detecting erythrocin |
| CN103995107A (en) * | 2013-02-19 | 2014-08-20 | 北京勤邦生物技术有限公司 | Detection method for lincomycin and special test paper therefor |
| CN104678069A (en) * | 2013-11-27 | 2015-06-03 | 中国农业大学 | Kanamycin, erythrocin and lincomycin three-in-one micro-hole gold-labeled test strip |
| CN105116148A (en) * | 2014-09-17 | 2015-12-02 | 北京勤邦生物技术有限公司 | Test strip and method for detecting sodium pentachlorophenate |
-
2016
- 2016-11-09 CN CN201610983619.8A patent/CN106526189A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202710567U (en) * | 2012-06-15 | 2013-01-30 | 北京中检葆泰生物技术有限公司 | Colloidal gold test paper strip and kit both capable of rapidly detecting lincomycin |
| CN203069594U (en) * | 2012-10-22 | 2013-07-17 | 北京勤邦生物技术有限公司 | Colloidal gold test strip for detecting erythrocin |
| CN103995107A (en) * | 2013-02-19 | 2014-08-20 | 北京勤邦生物技术有限公司 | Detection method for lincomycin and special test paper therefor |
| CN104678069A (en) * | 2013-11-27 | 2015-06-03 | 中国农业大学 | Kanamycin, erythrocin and lincomycin three-in-one micro-hole gold-labeled test strip |
| CN105116148A (en) * | 2014-09-17 | 2015-12-02 | 北京勤邦生物技术有限公司 | Test strip and method for detecting sodium pentachlorophenate |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107037216A (en) * | 2017-04-17 | 2017-08-11 | 新疆维吾尔自治区疾病预防控制中心 | The method of lincomycin in immunochromatographic measurement milk is quenched based on background fluorescence |
| CN108165532A (en) * | 2017-12-27 | 2018-06-15 | 江南大学 | One plant of woods can amine versatility monoclonal antibody hybridoma cell strain and its application |
| CN108165532B (en) * | 2017-12-27 | 2020-08-04 | 江南大学 | Lincosamine universal monoclonal antibody hybridoma cell strain and application thereof |
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Application publication date: 20170322 |