CN106525948B - A kind of preparation method and application of molecular engram detection 2,4-d sensor - Google Patents
A kind of preparation method and application of molecular engram detection 2,4-d sensor Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 68
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 34
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 18
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000011259 mixed solution Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 150000004780 naphthols Chemical class 0.000 claims abstract description 11
- 238000001548 drop coating Methods 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 230000008021 deposition Effects 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 6
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000008367 deionised water Substances 0.000 claims abstract description 4
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 4
- 238000005137 deposition process Methods 0.000 claims abstract description 4
- 229910021397 glassy carbon Inorganic materials 0.000 claims abstract description 4
- 238000002604 ultrasonography Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 14
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 13
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 claims description 13
- -1 2,4- Dichlorophenoxy Chemical group 0.000 claims description 11
- 239000000276 potassium ferrocyanide Substances 0.000 claims description 11
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 claims description 11
- 239000002270 dispersing agent Substances 0.000 claims description 10
- 239000001103 potassium chloride Substances 0.000 claims description 10
- 235000011164 potassium chloride Nutrition 0.000 claims description 10
- 238000001903 differential pulse voltammetry Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 2
- XEYINGFRHZCVKK-UHFFFAOYSA-N 2,2-dichloro-2-phenoxyacetic acid Chemical compound OC(=O)C(Cl)(Cl)OC1=CC=CC=C1 XEYINGFRHZCVKK-UHFFFAOYSA-N 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical group [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 238000000083 pulse voltammetry Methods 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000002203 pretreatment Methods 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical group [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- VDGJOQCBCPGFFD-UHFFFAOYSA-N oxygen(2-) silicon(4+) titanium(4+) Chemical compound [Si+4].[O-2].[O-2].[Ti+4] VDGJOQCBCPGFFD-UHFFFAOYSA-N 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920000128 polypyrrole Polymers 0.000 description 1
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
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- Health & Medical Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
A kind of preparation method and application of molecular engram detection 2,4-d sensor, is related to preparation and the applied technical field of molecular engram sensor.It disperses classifying porous silica spheres in deionized water under ultrasound condition, then drop coating is in clean glassy carbon electrode surface, after drying drop coating naphthols again, obtains the classifying porous silica ball electrode of naphthols protection；The classifying porous silica ball electrode that naphthols is protected is placed in by 2; it is placed in the mixed solution being made of methanol and acetic acid again after carrying out deposition processes in the deposition liquid of 4-d and o-phenylenediamine composition, elutes 2,4- dichlorphenoxyacetic acid; it obtains molecular engram and detects 2,4-d sensor.The present invention can more 2, the 4-d molecular locus of trace, to improve the sensitivity of detection, in addition, classifying porous silica spheres have preferable biocompatibility, raw material is easy to get, it is low in cost the features such as.
Description
Technical field
The present invention relates to the preparation of molecular engram sensor and applied technical fields.
Background technique
2,4-d(2,4- dichlorphenoxyacetic acid) it is a kind of pesticide wide using region, application time is long, once as grinding
Study carefully the model compound of microbial degradation chlorinated aromatic compound, and a kind of artificial synthesized plant hormone.2,4-d is similar to
Auxin or other plant growth regulator, can be absorbable with stimulating growth, rejuvenation cell, the root of plant, leaf, passes through stimulation
Nucleic acid and protein synthesis etc. influence plant metabolism.But 2,4-d be environment incretion interferent, thanks to intermediate production containing chloro
Object is easy to accumulate in vivo, and residual minim medicament will cause certain harm, so the safety in order to determine food, leads to
Often need to test and analyze the 2,4-d content in plant.
Mainly there are gas-chromatography, high performance liquid chromatography, an immunization to the method for the detection of 2,4-d at present, gas chromatography mass spectrometry, this
A little methods are pre-processed using organic solvent extracting and enriching, so should must have the sample pre-treatments of complexity, using toxic solvent,
And need the instrument of valuableness and the talent Jing Guo special training that can operate.
Summary of the invention
Object of the present invention is to propose a kind of preparation method of the sensor of molecular engram that can be used for facilitating detection 2,4-d.
The present invention the following steps are included:
1) it under ultrasound condition, disperses classifying porous silica spheres in deionized water, obtains classifying porous titanium dioxide
Silicon ball water dispersant；
2) by classifying porous silica spheres water dispersant drop coating in clean glassy carbon electrode surface, drop coating again after drying
Naphthols obtains the classifying porous silica ball electrode of naphthols protection；
3) the classifying porous silica ball electrode that naphthols is protected is placed in by 2,4-d (2,4 dichlorophenoxyacetic acid) and
It is placed in the mixed solution being made of methanol and acetic acid again after carrying out deposition processes in the deposition liquid of o-phenylenediamine composition, elution 2,
4- dichlorphenoxyacetic acid obtains molecular engram and detects 2,4-d sensor.
The present invention wraps up naphthalene in classifying porous silica ball surface using classifying porous silica spheres as matrix
Phenol can prevent classifying porous silica spheres from falling off from the surface of electrode.
The classifying porous silica spheres of the present invention have high specific surface area, can more 2, the 4-d molecular locus of trace,
To improve the sensitivity of detection, in addition, classifying porous silica spheres have preferable biocompatibility, raw material is easy to get, at
The features such as this is cheap.
Molecular engram sensor is due to easy to operate, the features such as pre-treatment that sample should not be complicated is quick, and stability is good,
It is receive more and more attention, study (Xie C, Gao S, Guo Q, et al. Electrochemical sensor
for 2,4-dichlorophenoxy acetic acid using molecularly imprinted polypyrrole
Membrane as recognition element. Microchimica Acta, 2010,169 (1): 145-152.) table
Bright, molecular engram can be used for detecting 2,4-d, and classifying porous silica spheres binding molecule trace is detected 2,4-d can be very
Detection limit is improved in big degree, therefore is of great significance.
Further, the partial size of classifying porous silica spheres of the present invention is 150~200nm.Replant the institute of type
There is silicon ball to have the 3D porous structure of interconnection, the presence in a large amount of nanometer sites provides big surface to the absorption of object
Product, this kind of design feature are conducive to raising 2, the sensitivity of 4-d measurement.
The concentration of classifying porous silica spheres is 8mg/mL in the classifying porous silica spheres water dispersant.Selection
The silicon ball of the concentration can provide enough areas for molecular engram site, while will not hinder the transmitting of electronics, to improve
Detection sensitivity.The concentration too low energy of classifying porous silica spheres enough prints such as in classifying porous silica spheres water dispersant
The molecule of mark is with regard to fewer, but excessive concentration can then hinder the transmitting of electronics.Therefore, classifying porous titanium dioxide of the present invention
The concentration of classifying porous silica spheres is 8mg/mL in silicon ball water dispersant.
In addition, 2,4-d amount determine molecular engram site amount, such as selection concentration cross urine cannot be formed it is enough
Site brings unnecessary waste if the excessive concentration selected.Therefore, the present invention in the deposition liquid 2,4-d it is dense
Degree is 10 mmol/L.
In addition, the application the invention also provides the above sensor in 2,4- dichlorphenoxyacetic acid concentration determination, that is, examine
Survey method.
Detection method includes the following steps:
1) sensor is placed in the mixed solution being made of the potassium ferricyanide and potassium ferrocyanide of potassium chloride, utilizes differential
Pulse voltammetry measures initial peak point current (I when no 2,4-d0)；
2) sensor is respectively placed in again at least five groups of 2,4-d (2,4 dichlorophenoxyacetic acid) solution of various concentration
It takes out, then is placed in the mixed solution being made of the potassium ferricyanide and potassium ferrocyanide of potassium chloride after impregnating 4min, utilize differential
Pulse voltammetry measures the initial peak point current (I) corresponding to different 2,4-d (2,4 dichlorophenoxyacetic acid) concentration；
3) current differential (I is made0- I) and corresponding 2,4 dichlorophenoxyacetic acid log concentration linear relationship chart；
4) sensor is placed in concentration to be measured 2, is taken out after impregnating 4min in 4-d solution, then be placed in the iron by potassium chloride
In the mixed solution of potassium cyanide and potassium ferrocyanide composition, concentration 2,4-d solution to be measured is measured using Differential Pulse Voltammetry
Initial peak point current；
5) it is conducive to current differential (I0- I) and the linear relationship chart of corresponding 2,4 dichlorophenoxyacetic acid log concentration check in
The concentration value of 2,4-d in concentration 2,4-d solution to be measured corresponding to the initial peak point current of concentration 2,4-d solution to be measured.
Electrode made of above method of the present invention is placed on after being impregnated in 2,4-d solution, since imprinted sites are occupied,
It is that peak current reduces, as 2,4-d concentration increases, site is occupied more, and electric current is smaller, utilizes current differential and 2,4-d
The linear relationship of log concentration is, it can be achieved that 2, the detection of 4-d.The present invention is detected more convenient using electrochemical method.
Carrying out the detection of 2,4- dichlorphenoxyacetic acid using the present invention can be improved detection limit, and instrument price is cheap, no
Complicated sample pre-treatments are needed, testing cost can be largely reduced, save detection time.
Detailed description of the invention
Fig. 1 is the linear relationship chart of the current differential and 2,4-d log concentration that are made of inventive sensor.
Specific embodiment
Below with reference to the embodiments and with reference to the accompanying drawing the technical solutions of the present invention will be further described.
One, sensor is prepared:
1, it under ultrasound condition, disperses the classifying porous silica spheres of 8mg that partial size is 150~200nm in 1 mL water,
The water dispersant of classifying porous silica spheres is obtained, wherein the concentration of classifying porous silica spheres is 8mg/mL.
2, take the water dispersant drop coating of the 5 classifying porous silica spheres of μ L in clean glassy carbon electrode surface, natural airing
One layer of naphthols of drop coating again afterwards obtains the classifying porous silica ball electrode of naphthols protection.
3, the classifying porous silica ball electrode that naphthols is protected is placed in by 2,4-d (2,4 dichlorophenoxyacetic acid) and
It is placed in the mixed solution being made of methanol and acetic acid again after carrying out deposition processes in the deposition liquid of o-phenylenediamine composition, elution 2,
4- dichlorphenoxyacetic acid obtains molecular engram and detects 2,4-d sensor.
The concentration of 2,4-d is 10mol/L in the above deposition liquid.
Methanol and the acetic acid mixing that the mixed solution being made of above methanol and acetic acid is 8: 1 by volume ratio form.Similarly hereinafter.
Two, the linear relationship chart of current differential and 2,4-d log concentration is made:
1, the mixed solution being made of the potassium ferricyanide and potassium ferrocyanide of potassium chloride is prepared:
Potassium chloride, the potassium ferricyanide and potassium ferrocyanide are mixed with the ratio that volume ratio is 2: 1: 1.
2, different 2,4-d concentration liquids are configured:
Not same amount 2 are taken, 4-d is dissolved in deionized water, and being respectively formed concentration is 1.0 × 10-10, 2.5 × 10-10, 5.0 ×
10-10, 7.5 × 10-10, 1.0 × 10-9, 2.5 × 10-9, 5.0 × 10-9, 7.5 × 10-9, 1.0 × 10-8, 2.5 × 10-8With 5.0
×10-8The 2,4-d solution of mol/L.
3, sensor obtained is placed in the mixed solution being made of the potassium ferricyanide and potassium ferrocyanide of potassium chloride, benefit
Initial peak point current I when 2,4-d concentration is 0 is measured with Differential Pulse Voltammetry0。
4, by sensor obtained in a kind of above ten 2,4-d of various concentration (2,4 dichlorophenoxyacetic acid) solution
It takes out, then is placed in the mixed solution being made of the potassium ferricyanide and potassium ferrocyanide of potassium chloride after impregnating 4min, utilize differential
Pulse voltammetry measures the initial peak point current I corresponding to different 2,4-d (2,4 dichlorophenoxyacetic acid) concentration1、I2、I3、I4、
I5、I6、I7、I8、I9、I10、I11。
5, each current differential: I is acquired respectively0- I1、I0- I2、I0- I3、I0- I4、I0- I5、I0- I6、I0- I7、I0?
I8、I0- I9、I0- I10、I0- I11.And by each current differential and corresponding 2,4-d (2,4 dichlorophenoxyacetic acid) log concentration
Linear relationship chart is made in value, as shown in Figure 1.
Three, sample detects:
In order to investigate the achieved reliability of this method, the detection of tall scrawny person's sample is carried out, due to through in detection tall scrawny person
Without 2,4-d, so being detected using standard samples recovery.
Adding 2,4-d respectively in tall scrawny person and being formed containing concentration is 1 × 10-9mol/L、5×10-9Mol/L and 1 ×
10-8Three groups of samples containing 2,4-d of mol/L.
By sensor obtained respectively in three above 2, the sample of 4-d concentration impregnate 4min after take out, then be placed in by
In the mixed solution of the potassium ferricyanide and the potassium ferrocyanide composition of potassium chloride, initial peak current is measured using Differential Pulse Voltammetry
Value.
By the linear relationship chart of Fig. 1,2, the 4-d concentration value for obtaining sample to be tested, 2,4-d, mono- concentration of three samples are searched
Detected value is respectively 9.44 × 10-10mol/L、5.39×10-9Mol/L and 9.53 × 10-9Mol/L, as a result as shown in the table:
As seen from the above table: it can be convenient using inventive sensor, rapidly and accurately measure, 2,4-d concentration.
The present invention can overcome traditional detection 2,4-d thus have complex sample pre-treatment, toxic solvent, and need
The disadvantages of instrument of valuableness, testing cost is greatly reduced, the efficiency of detection is improved.
Claims (3)
1. a kind of preparation method of molecular engram detection 2,4- dichlorphenoxyacetic acid sensor, it is characterised in that including following step
It is rapid:
1) it under ultrasound condition, disperses classifying porous silica spheres in deionized water, obtains classifying porous silica spheres
Water dispersant；The concentration of classifying porous silica spheres is 8mg/mL in the classifying porous silica spheres water dispersant；
2) by classifying porous silica spheres water dispersant drop coating in clean glassy carbon electrode surface, drop coating naphthalene again after drying
Phenol obtains the classifying porous silica ball electrode of naphthols protection；
3) the classifying porous silica ball electrode that naphthols is protected is placed in and is made of 2,4 dichlorophenoxyacetic acid and o-phenylenediamine
Deposition liquid in carry out deposition processes after be placed in the mixed solution being made of methanol and acetic acid again, elute 2,4- Dichlorophenoxy second
Acid obtains molecular engram and detects 2,4- dichlorphenoxyacetic acid sensor；2,4 dichlorophenoxyacetic acid is dense in the deposition liquid
Degree is 10 mmol/L.
2. the preparation method of molecular engram detection 2,4- dichlorphenoxyacetic acid sensor according to claim 1, feature
The partial size for being the classifying porous silica spheres is 150~200 nm.
3. the sensor of method preparation as described in claim 1 is used for the detection method of 2,4- dichlorphenoxyacetic acid concentration, special
Sign be the following steps are included:
1) sensor is placed in the mixed solution being made of the potassium ferricyanide and potassium ferrocyanide of potassium chloride, utilizes differentiated pulse
Voltammetry measures initial peak point current when no 2,4 dichlorophenoxyacetic acid；
2) sensor is respectively placed in after impregnating 4min at least five groups of 2,4 dichlorophenoxyacetic acid solution of various concentration again and is taken
Out, it then is placed in the mixed solution being made of the potassium ferricyanide and potassium ferrocyanide of potassium chloride, is surveyed using Differential Pulse Voltammetry
Obtain the initial peak point current corresponding to different 2,4 dichlorophenoxyacetic acid concentration；
3) linear relationship chart of current differential and corresponding 2,4 dichlorophenoxyacetic acid log concentration is made；
4) sensor is placed in concentration to be measured 2, is taken out after impregnating 4min in 4- dichlorphenoxyacetic acid solution, then be placed in by chlorination
In the mixed solution of the potassium ferricyanide and the potassium ferrocyanide composition of potassium, concentration to be measured 2,4- is measured using Differential Pulse Voltammetry
The initial peak point current of dichlorphenoxyacetic acid solution；
5) it is to be measured to check in concentration for the linear relationship chart conducive to current differential and corresponding 2,4 dichlorophenoxyacetic acid log concentration
2 in the 2,4 dichlorophenoxyacetic acid solution to be measured of concentration corresponding to the initial peak point current of 2,4 dichlorophenoxyacetic acid solution,
The concentration value of 4- dichlorphenoxyacetic acid.
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