CN106520722A - Application of casein kinase PPK related to growth and development of plants - Google Patents

Application of casein kinase PPK related to growth and development of plants Download PDF

Info

Publication number
CN106520722A
CN106520722A CN201610945892.1A CN201610945892A CN106520722A CN 106520722 A CN106520722 A CN 106520722A CN 201610945892 A CN201610945892 A CN 201610945892A CN 106520722 A CN106520722 A CN 106520722A
Authority
CN
China
Prior art keywords
casein kinase
growth
ppk
development
plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610945892.1A
Other languages
Chinese (zh)
Inventor
柳青
蔡大伟
黄颖
王雪君
陈芷馨
高红
孙世城
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujian Agriculture and Forestry University
Original Assignee
Fujian Agriculture and Forestry University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujian Agriculture and Forestry University filed Critical Fujian Agriculture and Forestry University
Priority to CN201610945892.1A priority Critical patent/CN106520722A/en
Publication of CN106520722A publication Critical patent/CN106520722A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11001—Non-specific serine/threonine protein kinase (2.7.11.1), i.e. casein kinase or checkpoint kinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention provides an application of a casein kinase PPK related to the growth and development of plants. The casein kinase PPK is PPK1, PPK2, PPK3 or PPK4, and the sequence registration numbers thereof are At3G13670, At5G18190, At3G03940 and At2G25760 respectively. The casein kinase PPK is used for improving the growth and development of the plants, and then is applied to crop production.

Description

The application of the one class casein kinase PPK related to growth and development of plants
Technical field
The invention belongs to biological technical field, and in particular to the class casein kinase PPK related to growth and development of plants Application.
Background technology
Protein kinase be a class using GTP or ATP as phosphodonor, γ-phosphate group is transferred to into serine, threonine Or on tyrosine, phosphorylating protein, so as to adjust coherent signal approach.Eukaryotic protein kinase is one very big same Source protein superfamily, comprising A-C-G groups, CaMK groups, C-M-G-C groups, routine protein-casein kinase group and other Protein kinase family (Hanks and Hunter 1995).
In the middle period after 19th century, researcher has one kind in first time finding rat liver being capable of the caseic egg of phosphorylation White kinases, is named as casein kinase (Burnett and Kennedy 1954).Since then, casein kinase causes people's Extensive concern.At present, relative to the research of casein kinase in animal, in plant, the research of casein kinase is more preliminary.
Evolution of the casein kinase in eucaryote is more guarded, many to adjust by combination or phosphorylated protein substrate Cell processes are planted, such as film transhipment, biological clock, cancer, DNA- repairs approach (Dhillon and Hoekstra 1994), Wnt (the Knippschild et al.2005 such as signal, cytoskeleton maintenance, RNA metabolism, parasitic infection;Liu et al.2009; Liu et al.2003;Venerando et al.2014).But the function in plant also studies less, Liu etc.（2003）Send out Existing paddy rice Casein kinase 1 has effect (Liu et al. 2003) in terms of root development and hormone response.
The content of the invention
It is an object of the invention to provide the application of the class casein kinase PPK related to growth and development of plants, probes into A class homologous casein kinase in arabidopsis（At3G13670, At5G18190, At3G03940, At2G25760）Physiology work( Can, whereby improving the growth and development of plant, and then application is into crop production.
For achieving the above object, the present invention is adopted the following technical scheme that：
（1）In order to realize the purpose of the present invention, the present invention constructs a recombinant plant expression vector first, described recombinant expressed Carrier isACT2::2*Flag-GFP-linker-PPK。
Wherein, linker is formed by 5 amino acid tandems, and its sequence is：PAPAP；GFP is green fluorescent protein.
The construction method of the carrier is as follows：
A. find At3G13670 in tair databases, At5G18190, At3G03940 and At2G25760 this four genes, because It is that this laboratory is obtained when arabidopsis blue light receptor CRY2 screens yeast cDNA library for such protein kinase, therefore is ordered Entitled PPK1, PPK2, PPK3 and PPK4.Abbreviations of the PPK for Photoregulatory Protein Kinases.According to its sequence Row design pcr amplification primer thing pair, as shown in table 1；
B. with the total cDNA of wild Col-0 arabidopsis as template, enter the complete sequence that performing PCR obtains 4 casein kinases；
C. PCR fragment is connected in plant expression vector pFGFP, collection of illustrative plates such as Fig. 1.Jing sequencing identifications are obtained and genes of interest Identical sequence；
D. agriculture bacillus mediated method is adopted, to arabidopsisrdr6-11(Peragine et al. 2004) plant carries out dipping in flower Infect.The seed of plant after collection conversion, and carry out antiweed（Basta）Screening, and then verified by western blot Obtain transgenic positive plant.
E. phenotype analytical, phenotype such as Fig. 4, shown in Fig. 6 are carried out to positive transgenic plant.
（2）In order to preferably illustrate the function of PPKs, the present invention also constructs polygenes interference carrier, the interference carrier For：Act2::amiRPPK1 /Ubq10::amiRPPK4(Bar) andAct2::amiRPPK2 /Ubq10::amiRPPK3 (Hygromycin)。
A. according to At3G13670, the sequence of At5G18190, At3G03940 and At2G25760 is designed for which Artificial microRNA, its nucleotide sequence such as table 2.With reference to method (the Li et al. in Jen sheen laboratories 2013) artificial microRNA are cloned（amiR）Skeleton.And willamiRPPK1WithamiRPPK4Connect into bivalent carrier PDT1-Bar, willamiRPPK2WithamiRPPK3Connect into bivalent carrier pDT1-Hyg.PDT1-Bar Vector maps such as Fig. 2. PDT1-Hyg Vector maps such as Fig. 3.
B., after being sequenced correctly, carrier is proceeded in Agrobacterium Agl0 respectively.And will containAct2::amiRPPK1/ Ubq10::amiRPPK4(Bar) andAct2::amiRPPK2/Ubq10::amiRPPK3(Hygromycin) agriculture of interference carrier Bacillus cotransformation arabidopsis wild type Col-0.The seed of plant after collection conversion, and carry out antiweed（Basta）And tide The Double screening of mycin, so by RT-PCR method checking obtain transgenic positive plant, and will this at the same disturb four The transfer-gen plant of gene is named asamiR 4k 。
C. to disturbing plant to carry out phenotype analytical, phenotype such as Fig. 5, shown in Fig. 7.
Table 1.PPK1,PPK2,PPK3WithPPK4For the primer sequence of clone
Table 2.PPK1,PPK2,PPK3WithPPK4The sequence of the artificaial microRNA of design
It is an advantage of the current invention that：The important LCK of a class is studied in the present invention, protein kinase is different by adjusting Substrate function, and during these substrates are likely to be at different plant signaling transduction approach and affect plant growth send out Educate.Conventional research is simply started with from these single substrates, studies its impact to growth and development of plants, and plant trait changes May be not notable.And the present invention starts with from protein kinase, change its expression and can reach while changing its numerous substrate Activity further has influence on the different aspect of growth and development of plants, and concrete phenotype this patent is enumerated out, such as changes under plant Plumular axis length, blade shape and flowering time.
Description of the drawings
Fig. 1 is pFGFP Vector maps in the present invention.
Fig. 2 is pDT1-Bar Vector maps in the present invention.
Fig. 3 is pDT1-Hyg Vector maps in the present invention.
Fig. 4 is the comparison of transgenic line and wild type hypocotyl length in the present invention.Wherein WT is arabidopsisrdr6- 11,cry1For blue light receptor cryptochrome1 deletion mutants,cry2For blue light receptor cryptochrome2 deletion mutations Body,cry1cry2For double-mutant,Actin2::PPK1For transgenic line.
Fig. 5 is the comparison of interference strain and wild type hypocotyl length in the present invention.Wherein WT is arabidopsis Col-4,cry1cry2For cryptochrome double-mutant,phyAphyBFor phytochrome double-mutant, amiR4kFor PPK1, PPK2, PPK3 and PPK4 polygenes disturbs strain.
Fig. 6 is the comparison of transgenic line and wild-type leaves shape in the present invention.Wherein control is carried on the back for transfer-gen plant Scape strainrdr6-11, Actin2::PPK1,Actin2::PPK2, Actin2::PPK3, Actin2::PPK4For transgenic line System.
Fig. 7 is the comparison of interference strain and wild type flowering time in the present invention.Wherein WT is arabidopsis Col-4, amiR4k For PPK1, PPK2, PPK3 and PPK4 polygenes interference strain.
Specific embodiment
Embodiment 1
First, according toPPK1Sequences Design pcr amplification primer thing pair, as shown in table 1.With the total cDNA of wild Col-0 arabidopsis as mould Plate, enters performing PCR acquisitionPPK1Complete sequence.PCR fragment is connected in plant expression vector pFGFP, collection of illustrative plates such as Fig. 1.Jing is sequenced Identification is obtained and the identical sequence of genes of interest.Then, using agriculture bacillus mediated method, to arabidopsisrdr6-11 (Peragine et al. 2004)Plant carries out dipping in colored infecting.The seed of plant after collection conversion, and carry out antiweed （Basta）Screening, and then transgenic positive plant is obtained by western blot checkings, finally obtaining 3 has turning for expression Gene strain.With wild type and PPK transgenic arabidopsis as material, light process is carried out（Blue light：30 μm of olm-2s-1, ruddiness： 30, far-red light：2.5, it is dark）, after growing 5 days, hypocotyl is measured.Measurement data is shown in Table 3.
Impacts of 3. PPK1 of table to arabidopsis hypocotyl growth（mm）
Note：Colleague's data shoulder note same letter represents that difference is not notable（P ＞ 0.05）, the different letter representation significant differences of shoulder note（p ＜ 0.05）.
After the white kinases overexpression of its excess-three hatching egg, hypocotyl phenotype is basically identical with PPK1, and the lower hypocotyl of light process is higher than Control group.There is functional redundancy phenomenon in this four protein kinases, therefore not herein by its overexpression strain hypocotyl phenotype Data all show, and illustrate the phenotype of polygenes interference strain as described below to further elucidate such protein kinase pair The impact of hypocotyl growth.
The present invention also constructs polygenes interference carrier, according to At3G13670, At5G18190, At3G03940 and The sequence of At2G25760, designs artificial microRNA for which, its nucleotide sequence such as table 2.With reference to Jen sheen realities Test method (Li et al. 2013) the clone artificial microRNA of room（amiR）Skeleton.And willamiRPPK1WithamiRPPK4Connect into bivalent carrier pDT1-Bar, willamiRPPK2WithamiRPPK3Connect into bivalent carrier pDT1-Hyg. PDT1-Bar Vector maps such as Fig. 2.PDT1-Hyg Vector maps such as Fig. 3.
After sequencing is correct, carrier is proceeded in Agrobacterium Agl0 respectively.And will containAct2::amiRPPK1/ Ubq10:: amiRPPK4(Bar) andAct2::amiRPPK2/Ubq10::amiRPPK3(Hygromycin) Agrobacterium of interference carrier is common With arabidopsis thaliana transformation wild type Col-0.The seed of plant after collection conversion, and carry out antiweed（Basta）It is double with hygromycin Resistance screening, and then transgenic positive plant is obtained by RT-PCR method checking, and will be this while disturbing four genes Transfer-gen plant is named asamiR 4k 。
Disturb strain as material with wild type and ppk, carry out light process（Blue light：20, ruddiness：20, far-red light：2.5, it is black Secretly）, after growing 5 days, hypocotyl is measured.Measurement data is shown in Table 4. with PPK overexpression phenotype conversely, ppk interference strains Show hypocotyl under light is processed and be shorter than wild type.
Table 4.PPK interference strain hypocotyl growth changes（mm）
Note：Colleague's data shoulder note same letter represents that difference is not notable（P ＞ 0.05）, the different letter representation significant differences of shoulder note（p ＜ 0.05）.
Strain is disturbed as material with wild type and ppk, under long-day conditions（16h illumination/8h is dark）When being bloomed Between count.Statistics is shown in Table flowering time under the conditions of 5. ppk interference strain shows to grow day and postpones.
Table 5.PPK interference strain flowering time changes
Note：Colleague's data shoulder note same letter represents that difference is not notable（P ＞ 0.05）, the different letter representation significant differences of shoulder note（p ＜ 0.05）.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with Modification, should all belong to the covering scope of the present invention.
SEQUENCE LISTING
<110>University Of Agriculture and Forestry In Fujian
<120>The application of the one class casein kinase PPK related to growth and development of plants
<130> 12
<160> 12
<170> PatentIn version 3.3
<210> 1
<211> 32
<212> DNA
<213>Artificial sequence
<400> 1
tccagctcca ggatccatgc cggagcttcg cc 32
<210> 2
<211> 38
<212> DNA
<213>Artificial sequence
<400> 2
gagaaagctt ggatcctcaa gatacagttc ggccatag 38
<210> 3
<211> 39
<212> DNA
<213>Artificial sequence
<400> 3
tccagctcca ggatccatgc cagagttaag aagtggagc 39
<210> 4
<211> 36
<212> DNA
<213>Artificial sequence
<400> 4
gagaaagctt ggatcctcag caaactgtcc tcccaa 36
<210> 5
<211> 39
<212> DNA
<213>Artificial sequence
<400> 5
tccagctcca ggatccatgc cagagttaag aagtggagc 39
<210> 6
<211> 38
<212> DNA
<213>Artificial sequence
<400> 6
gagaaagctt ggatcctcag caaactgtcc gaccatag 38
<210> 7
<211> 34
<212> DNA
<213>Artificial sequence
<400> 7
tccagctcca ggatccatgc ctgagctgcg tagc 34
<210> 8
<211> 39
<212> DNA
<213>Artificial sequence
<400> 8
gagaaagctt ggatcctcat gacacagttc gaccataac 39
<210> 9
<211> 21
<212> DNA
<213>Artificial sequence
<400> 9
acgcaatgga tatcgattta c 21
<210> 10
<211> 21
<212> DNA
<213>Artificial sequence
<400> 10
aagtgggtat aggataacat c 21
<210> 11
<211> 21
<212> DNA
<213>Artificial sequence
<400> 11
tcgagccatg tgctatatct a 21
<210> 12
<211> 21
<212> DNA
<213>Artificial sequence
<400> 12
cagggcgatt tttacgtgat g 21

Claims (2)

1. the class casein kinase PPK related to growth and development of plants is changing plant hypocotyl length, blade shape and opens Application in taking time.
2. application according to claim 1, it is characterised in that：Described casein kinase PPK is PPK1, PPK2, PPK3 Or PPK4, its Sequence accession number is respectively At3G13670, At5G18190, At3G03940 and At2G25760.
CN201610945892.1A 2016-10-26 2016-10-26 Application of casein kinase PPK related to growth and development of plants Pending CN106520722A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610945892.1A CN106520722A (en) 2016-10-26 2016-10-26 Application of casein kinase PPK related to growth and development of plants

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610945892.1A CN106520722A (en) 2016-10-26 2016-10-26 Application of casein kinase PPK related to growth and development of plants

Publications (1)

Publication Number Publication Date
CN106520722A true CN106520722A (en) 2017-03-22

Family

ID=58292287

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610945892.1A Pending CN106520722A (en) 2016-10-26 2016-10-26 Application of casein kinase PPK related to growth and development of plants

Country Status (1)

Country Link
CN (1) CN106520722A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235129A (en) * 2020-01-21 2020-06-05 天津市职业大学 PPK2 protein and application thereof in polyacrylamide gel electrophoresis 30kd standard substance

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110179519A1 (en) * 2007-03-23 2011-07-21 Gloria Coruzzi Methods of Affecting Nitrogen Assimilation In Plants
CN105440116A (en) * 2015-12-28 2016-03-30 上海交通大学 Eggplant cryptochrome gene SmCRY2 and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110179519A1 (en) * 2007-03-23 2011-07-21 Gloria Coruzzi Methods of Affecting Nitrogen Assimilation In Plants
CN105440116A (en) * 2015-12-28 2016-03-30 上海交通大学 Eggplant cryptochrome gene SmCRY2 and application thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HE HUANG,ET AL: "Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry", 《MOLECULAR & CELLULAR PROTEOMICS》 *
SHU-TANG TAN,ET AL: "Arabidopsis Casein Kinase1 Proteins CK1.3 and CK1.4 Phosphorylate Cryptochrome2 to Regulate Blue Light Signaling", 《THE PLANT CELL》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235129A (en) * 2020-01-21 2020-06-05 天津市职业大学 PPK2 protein and application thereof in polyacrylamide gel electrophoresis 30kd standard substance

Similar Documents

Publication Publication Date Title
Dai et al. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis
Onai et al. PHYTOCLOCK 1 encoding a novel GARP protein essential for the Arabidopsis circadian clock
Liu et al. Overexpression of maize SDD1 (ZmSDD1) improves drought resistance in Zea mays L. by reducing stomatal density
Moussian et al. Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis
Sano et al. KNOX homeobox genes potentially have similar function in both diploid unicellular and multicellular meristems, but not in haploid meristems
Xu et al. EMBRYONIC FACTOR 1 encodes an AMP deaminase and is essential for the zygote to embryo transition in Arabidopsis
Cai et al. Acid phosphatase gene GmHAD1 linked to low phosphorus tolerance in soybean, through fine mapping
Lian et al. Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene
Wang et al. Receptor-like cytoplasmic kinases PBL34/35/36 are required for CLE peptide-mediated signaling to maintain shoot apical meristem and root apical meristem homeostasis in Arabidopsis
Ren et al. Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis
Zhang et al. Knockout of the VPS22 component of the ESCRT-II complex in rice (Oryza sativa L.) causes chalky endosperm and early seedling lethality
Fang et al. Modulation of evening complex activity enables north-to-south adaptation of soybean
CN103613649A (en) Paddy rice leaf color control gene OscpSRP54 and protein encoded by same
Zhang et al. GmRAV1 regulates regeneration of roots and adventitious buds by the cytokinin signaling pathway in Arabidopsis and soybean
ES2343536T3 (en) PLANTS THAT HAVE IMPROVED GROWTH CHARACTERISTICS AND METHOD FOR THEIR DEVELOPMENT.
Lv et al. Expression profiling and functional characterization of a CBL-interacting protein kinase gene from Populus euphratica
CN111087457B (en) Protein NGR5 for improving nitrogen utilization rate and crop yield, and coding gene and application thereof
Qin et al. Q Dtbn1, an F‐box gene affecting maize tassel branch number by a dominant model
Zhang et al. Molecular characterization and expression analysis of small heat shock protein 17.3 gene from Sorbus pohuashanensis (Hance) Hedl. in response to abiotic stress
Wu et al. Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1. 1 and TaNAK1. 2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis
Wang et al. Natural variation of ZmLNG1 alters organ shapes in maize
Kwak et al. Diverse roles of glycine-rich RNA-binding protein 7 in the response of camelina (Camelina sativa) to abiotic stress
CN106520722A (en) Application of casein kinase PPK related to growth and development of plants
Yang et al. Melon yellow-green plant (Cmygp) encodes a Golden2-like transcription factor regulating chlorophyll synthesis and chloroplast development
Li et al. Functional characterization of a grape heat stress transcription factor VvHsfA9 in transgenic Arabidopsis

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170322

RJ01 Rejection of invention patent application after publication