CN106520719B - A kind of ω of S type-transaminase ATA-W12 and its gene and application - Google Patents
A kind of ω of S type-transaminase ATA-W12 and its gene and application Download PDFInfo
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Abstract
The present invention provides ω-transaminase ATA-W12 and its gene and the application of a kind of S type.The ω of the S type-transaminase ATA-W12 amino acid sequence is as shown in SEQ ID NO:2.Amplification has obtained a kind of ω-aminotransferase gene ata-w12 of S type to the present invention from cotton field soil metagenome for the first time, and construct the one plant of ω containing the S type-aminotransferase gene ata-w12 engineering strain, to realize ω-aminotransferase gene ata-w12 heterogenous expression of the S type, ω obtained-transaminase ATA-W12 has excellent enzymatic property.Above-mentioned ω-transaminase ATA-W12 and its engineering strain are also applied in the production of biological enzyme synthesis of chiral amine (S)-N-Boc-3- amino piperidine and other chiral amine products by the present invention, have the characteristics that concentration of substrate is high, reaction rate is fast, stereoselectivity is good.
Description
Technical field
The present invention relates to technical field of bioengineering, relate more specifically to a kind of S type ω-transaminase ATA-W12 and its
Gene and application.
Background technique
It is known as PLP enzyme using phosphopyridoxal pyridoxal phosphate (PLP) as the enzyme of coenzyme.It is tied according to known protein sequence and space
Structure, PLP enzyme can be divided into five major class.Wherein most belongs to the first kind, i.e. aspartate transaminase family, including aromatic amine turns ammonia
Enzyme, glycine acetyltransferase.The representative of other a few class PLP enzymes is successively are as follows: the tryptophan synthetase β race of the second class, third
The eucaryote ornithine decarboxylase of class, the D- glycine transaminase of the 4th class, the glycogen phosphorylase of the 5th class.All this
Five fermentoids are formed with the oligomer of homodimer or higher level.The activated centre of enzyme is located at the surface of subunit, and
The amino acid residue in each subunit contribution activated centre.In the bond area PLP, these enzymes have 2 conservative regions: one is used to
The lysine residue and a loop rich in glycine for being used to be associated with coenzyme phosphate group of schiff bases are formed with PLP.
Transaminase generally refers to the first kind or the 4th class PLP enzyme.In transamination reaction, amino group donor is total with PLP first
Valence is combined and is formed phosphoric acid Pyridoxamine (PMP), and subsequent PMP gives the amino acceptor containing carbonyl transamination, thus completes to turn ammonia
The circulation (as shown in Figure 1) of reaction and coenzyme.The characteristics of according to protein structure and sequence, first kind PLP enzyme can be divided into again
Five major class, i.e. transaminase are always divided into six major class.Wherein the transaminase of third class is most paid close attention to by people, the native amino of these enzymes
Donor substrate has a common feature: amino is located on the non alpha position of carboxyl.These enzymes are known as ω-transaminase.ω-transaminase
Constitute the overwhelming majority in third class transaminase.
Many biologically active substances or in which mesosome are all Chiral Amine, such as (R) -2- amino-4-phenyl butane
It is the precursor of depressor dilevalol, amphetamine is the chiral building block of potent antidepressants, (S) -2- amino -1- phenyl propyl ether
It is the chiral building block of herbicide O utlook.However, these Chiral Amines prepare most of or traditional chemical method, although changing
The reaction system of method is clear, but disadvantage is also very much, and environmental pollution is serious, and reaction chain is tediously long, and stereoselectivity is poor, special
It is not that the use of transiting state metal and the end processing sequences of complexity have increased considerably production cost.ω-transaminase enzyme process
Synthesis is because of its mild reaction condition, and good stereoselectivity, higher conversion ratio, the characteristics such as simple post-processing can be very
Good solves the above problems.2010, Christopher K.Savile et al. was turned using the ω-that protein engineering means obtain
Adnosine deaminase saltant type ATA-117 is used successfully in the enzymatic clarification of the main component sitagliptin of Novel antidiabetic Januvia
In technique, this technique obtains the Presidential Green Chemistry Challenge Awards of current year, this also indicates ω-transaminase in Chiral Amine
It is with important application prospects in synthesis.
Biocatalysis preparation Chiral Amine has 2 kinds of approach: 1. Enzymatic Resolution of Several Racemic amine (as shown in Figure 2)；2. enzymatic
Asymmetric syntheses Chiral Amine (as shown in Figure 3).The method of the first chiral resolution racemic amines is simple and easy, but its maximum conversion
Rate can only achieve 50%, and the reduction amination of precursor ketone increases production cost, this undoubtedly limits its industrial applications
Process.Second of asymmetry catalysis synthetic method is the hot spot studied at present, because its theoretical yield can reach 100%, but
It is the presence of serious balance inhibition.
Optically pure 3- amino piperidine (3APi) is a kind of very important medicine intermediate, is used to synthesize a series of
Biologically active drug, such as tosufloxacin, Clinafloxacin and cephalosporin analog derivative.Other contain optical voidness 3- amino piperidine
The compound of group be used to treat obesity and I type or type II diabetes, and for synthesizing treatment depression and spirit
The psychotropic agent of Split disease etc..The document report about synthesis 3- amino piperidine has much at present, and there are mainly three types of different roads
Line: replacing, and cyclisation, resolving agent is split.These methods have the shortcomings that one or more mostly: needing to use when azide substitution high
The tosylated ethyl alcohol of expensive 1-N protection, will pass through many and diverse step synthesis.Since substitution process is related to structure
The reversion of type, the S of no stereoselectivityN1 substitution process must then exclude, and avoid generating the opposite product of stereoselectivity.This
The method that a little documents provide, does not all use chromatographic technique to go the optical purity of detection product.As for cyclization, 1-N, 3-N
The 3- amino piperidine of protection can be cyclized to obtain by optically pure aspartic acid or glutamic acid, or the dione compounds by being cyclized
It restores obtained amino acid and is cyclized generation again.However, the obtained yield of all cyclizations is all relatively low, optical purity
It is very low.For example, by available (the S) -3- amino piperidine of two steps, but yield is very low by (S) -2,4-diamino-butanoic.
The problem of cyclization process maximum is that yield can only achieve 50%, and the processing in later period include resolving agent recycling and mapping
The recycling of body is time-consuming and laborious.The method of synthesis 3- amino piperidine is mainly chemical method at present, so prepared by Biocatalytic Conversion method
(S) -3- amino piperidine has good application value.
Summary of the invention
The object of the present invention is to provide a kind of ω of S type-transaminase ATA-W12 and its gene and applications, to solve existing
There is the missing for the biological enzyme that Chiral Amine is prepared in technology.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
According to an aspect of the present invention, a kind of ω-transaminase ATA-W12 of S type, amino acid sequence such as SEQ are provided
Shown in ID NO:2.
The ω-transaminase ATA-W12 derives from cotton field soil metagenome.
According to another aspect of the present invention, a kind of ω-aminotransferase gene ata-w12 of S type is also provided, base sequence is such as
Shown in SEQ ID NO:1.
A kind of recombinant plasmid, ω-aminotransferase gene ata-w12 and expression of the recombinant plasmid by the S type are also provided
Vector plasmid connection is built-up.
Preferably, which is pET-28a, can construction recombination plasmid ata-w12-pET-28a.
According to another aspect of the invention, a kind of ω-transaminase ATA-W12 engineering strain of S type is provided, described in
The ω of S type-transaminase ATA-W12 engineering strain is the genetic engineering bacterium for obtaining the recombinant plasmid transformed host strain
Strain.
The present invention also provides a kind of ω-transaminase ATA-W12 of S type and ω-transaminase ATA- of the S type
Application of the W12 engineering strain in synthesis of chiral amine product.
The ω of the S type-transaminase ATA-W12 catalytic temperature is 20~60 DEG C, most preferably 40 DEG C.
It is 8.0~10.0, most preferably 8.5 that the ω of the S type-transaminase ATA-W12 catalysis, which restores pH,.
Especially described ω-transaminase ATA-W12 and the engineering strain be used to be catalyzed N-Boc-3- piperidines
The application of ketogenesis (S)-N-Boc-3- amino piperidine.
Specifically, it is used for ω-transaminase ATA-W12 engineering strain to be catalyzed N-Boc- the present invention also provides a kind of
The application of the ketogenesis of 3- piperidines (S)-N-Boc-3- amino piperidine, the application include: by the ω of S type-transaminase ATA-W12 gene
The resulting ATA-W12 wet thallus of engineered strain culture carries out catalysis reaction: pH as catalyst in following reaction system
8.5Tris-HCl, ATA-W12 wet thallus, 500mM N-Boc-3- piperidones, 2mL DMSO, 1.5M are acidified isopropylamine, 0.2mM
PLP is in 40 DEG C of water-baths, and stirring, reaction terminates, and reaction solution is extracted with ethyl acetate, and collects extraction phase, and centrifugation takes organic phase
Vacuum rotary steam recycles ethyl acetate, obtains the crude product that pale yellow oily liquid is (S)-N-Boc-3- amino piperidine.
In short, amplification has obtained a kind of ω-aminotransferase gene of S type to the present invention from cotton field soil metagenome for the first time
Ata-w12, and the one plant of ω containing the S type-aminotransferase gene ata-w12 engineering strain is constructed, to realize
The ω of the S type-aminotransferase gene ata-w12 heterogenous expression, ω obtained-transaminase ATA-W12 have excellent zymetology
Characteristic, the temperature range of Study on Catalytic Amination of Alcohols are 20-60 DEG C, and Study on Catalytic Amination of Alcohols pH value is 8.0-10.0, saves 164h under the conditions of 40 DEG C
Enzyme activity not anti-reflection increases afterwards.In addition, the present invention also provides a kind of ω-transaminase ATA-W12 and the engineering strains
Application in synthesis of chiral amine product, especially ω-transaminase ATA-W12 and the engineering strain be used to urge
Change the application of the ketogenesis of N-Boc-3- piperidines (S)-N-Boc-3- amino piperidine.Biological catalysis list water phase of the invention prepares hand
Property amine (S)-N-Boc-3- amino piperidine, yield are up to 500mM/L (100g/L).Compared with traditional chemical method, which turns ammonia
Enzyme ATA-W12 genetic engineering bacterium have the characteristics that reaction rate it is fast, conveniently, concentration of substrate it is high.
Detailed description of the invention
Fig. 1 is ω-transaminase action principle figure；
Fig. 2 is the schematic diagram of Enzymatic Resolution of Several Racemic organic amine；
Fig. 3 is the schematic diagram of enzymatic asymmetric syntheses Chiral Amine；
Fig. 4 is ω-transaminase ATA-W12 SDS-PAGE figure that the present invention purifies, wherein M is albumen Marker, and 1 is
Destination protein ω-transaminase ATA-W12 after purification；
Fig. 5 is ω of the invention-transaminase ATA-W12 optimal reaction pH schematic diagram；
Fig. 6 is ω of the invention-transaminase ATA-W12 optimal reactive temperature schematic diagram；
Fig. 7 is ω of the invention-transaminase ATA-W12 thermal stability schematic diagram；
Fig. 8 is that ω of the invention-transaminase ATA-W12 engineering strain catalysis generates (S)-N-Boc-3- amino piperidine
Schematic diagram.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be further described.It should be understood that following embodiment is merely to illustrate this
The range of invention and is not intended to limit the present invention.
1. deriving from ω-aminotransferase gene ata-w12 amplification of soil metagenome
ω of the invention-aminotransferase gene ata-w12 derives from cotton field soil metagenome, and the soil metagenome is just
It is the genome that the pedotheque of the general cotton field acquisition in Shandong Province extracts, then obtained by macro gene order-checking.Based on NCBI
The homologous comparison of (https: //blast.ncbi.nlm.nih.gov/Blast.cgi), the inventor of the present application discovered that one with
Its similitude is up to 89% ω-transaminase from Caulobacter sp.K31.
As shown in SEQ ID NO:1, size is ω of the invention-aminotransferase gene ata-w12 nucleotide sequence
1407bp。
Amplification ω-aminotransferase gene ata-w12 primer is designed according to the sequencing results:
ata-w12F:5'AACCGGAATTCATGACCGCCCCCCTCCGCA 3'
ata-w12R:5'AACCCAAGCTTTCAGTCCTCGCCCTCCTTA 3'
Using above-mentioned cotton field soil metagenome as template, PCR amplification is carried out using the primer designed above, obtains ω-
Aminotransferase gene ata-w12.
2. the building containing ω-aminotransferase gene ata-w12 recombinant plasmid (ata-w12-pET-28a)
Of the invention containing ω-aminotransferase gene ata-w12 recombinant plasmid is by by ω-aminotransferase gene ata-
W12 connect built-up with optional plasmid pET-28a (as expression vector).The ω-aminotransferase gene ata-w12 and plasmid
The binding site of pET-28a is EcoR I and HindIII.Using soil metagenome as template, to design drawing containing restriction enzyme site
Object ata-w12F/ata-w12R obtains ω-aminotransferase gene ata-w12 by PCR amplification.
PCR amplification system is following (50 μ L): 2 × Taq Plus Master PCR Mix 25 μ L, primer 2 μ L, 2 μ of template
L adds ddH2O to complement to 25 μ L.PCR amplification condition: 94 DEG C of 5min；94 DEG C of 30sec, 55 DEG C of 30sec, 72 DEG C of 3min, 30 are followed
Ring；72℃5min.PCR product verifies size and glue recovery purifying by agarose gel electrophoresis.Specific steps according to
FAVORGEN DNA Ago-Gel QIAquick Gel Extraction Kit carries out double digestion at the genetic fragment of recycling and 37 DEG C of pET-28a plasmid.
It recycles endonuclease bamhi and is stayed overnight in 16 DEG C of connections, connection product is transferred to bacillus coli DH 5 alpha competent cell, is uniformly coated on
On kalamycin resistance plate, 16h is cultivated in 37 DEG C of inversions, and picking single colonie is verified as after positive colony after LB culture medium culture
Plasmid is extracted, the recombinant plasmid ata-w12-pET-28a of ω-aminotransferase gene ata-w12 and plasmid pET-28a are obtained.
3. the building containing ω-aminotransferase gene ata-w12 recombination engineered strain
The recombinant plasmid ata-w12-pET-28a that above-described embodiment 2 obtains is transferred to e. coli bl21 (DE3) competence
Cell (Tiangeng biology Co., Ltd), is uniformly coated on kalamycin resistance plate, and 16h, picking single bacterium are cultivated in 37 DEG C of inversions
It falls after being verified as positive colony and obtains recombination engineered strain through LB culture medium culture.
4. ω-transaminase ATA-W12 albumen expression and purifying
4.1 are inoculated in the recombination engineered strain of above-mentioned building containing LB (+50 μ g/mL kanamycin) culture medium
In test tube, 37 DEG C of shaking table culture 12h.
4.2 are inoculated into fresh liquid LB (+100 μ g/mL kanamycin) culture medium with 1% inoculum concentration, and 200
Rev/min lower 37 DEG C of revolving speed shake to OD [600]=0.6, add IPTG to 100 μM/mL, 20 DEG C of induction 20h.
4.3 bacterium solutions are centrifuged 5min in 4 DEG C, 8000rpm/min, collect thallus.Physiological saline (0.85%g/L NaCl) is clear
Wash primary, 4 DEG C, 8000rpm/min centrifugation 5min, collection thallus.By Tris-HCL (pH of the thallus containing 0.1mM PLP
8.5) it is resuspended, the ultrasonication in low temperature water-bath, after broken crude enzyme liquid 8000rpm is centrifuged 10min, draws supernatant Ni
NTA Beads (Smart-Lifesciences) purifying, desalination and concentration by ultrafiltration obtain destination protein, i.e. ω-transaminase ATA-
W12。
The destination protein of 4.4 pairs of acquisitions carries out SDS-PAGE, as a result as shown in figure 4, ω-transaminase ATA-W12 is in large intestine
It is expressed in bacillus, is after purification single band under TPI-250 (PLP containing 0.1mM) elution requirement.
The ω-transaminase ATA-W12 amino acid sequence is as shown in SEQ ID NO:2, and totally 468 amino acid, theoretical
Molecular weight is 51kDa.
5. ω-transaminase ATA-W12 optimal reaction pH value is analyzed
Recombination ω-transaminase ATA-W12 optimal reaction pH of the invention is measured within the scope of 6.0-10.5.That detects is anti-
Answer system (200 μ L) are as follows: different pH value buffer solutions, 2.5mM pyruvic acid and 2.5mM (S)-Alpha-Methyl benzylamine, 3.92 × 10-7g
Pure protein (recombinates ω-transaminase ATA-W12), reacts 5min under the conditions of 37 DEG C, measures absorption value under 245nm wavelength.It surveys
Buffer used in fixed are as follows: 100mM sodium phosphate buffer (pH 6.0-8.0), 100mM Tris-HCl buffer (pH 8.0-
9.0), 100mM glycine-NaOH buffer (pH 9.0-10.0).Measurement result is as shown in figure 5, show: recombination ω-transaminase
The optimal pH of ATA-W12 is the Tris-HCl buffer solution of pH 8.5, and greater activity is all had within the scope of pH8.0-10.0.
6. ω-transaminase ATA-W12 optimal reactive temperature is analyzed
Recombination ω-transaminase ATA-W12 optimal reactive temperature of the invention measures within the scope of 4-70 DEG C.That detects is anti-
Answer system (200 μ L) are as follows: 100mMTris-HCl buffer solution (pH 8.5), 2.5mM pyruvic acid and 2.5mM (S)-Alpha-Methyl benzyl
Amine, 3.92 × 10-7G pure protein (i.e. recombination ω-transaminase ATA-W12), respectively at 4,20,30,40,50,60,65,70 DEG C
5min is reacted, measurement result is as shown in fig. 6, find ω-transaminase ATA-W12 most to ATA-W12 optimal reactive temperature analysis
Suitable reaction temperature is that enzyme activity is almost the same at 40 DEG C, 40 DEG C and 50 DEG C, and enzymatic activity will not be with the change in reaction time at 40 DEG C
And reduce, and begun to decline greater than enzyme activity in 50 DEG C of reaction system, enzyme almost loses activity at 70 DEG C.In conclusion weight
Group ω-transaminase ATA-W12 optimal reactive temperature is 40 DEG C.
7. recombinating ω-transaminase ATA-W12 zymetology stability analysis
Recombination ω-transaminase ATA-W12 thermal stability measures within the scope of 40-70 DEG C, detection method are as follows: by purifying
ω-transaminase ATA-W12 enzyme solution is kept the temperature respectively under the conditions of 40,50,60,70 DEG C, and it is residual to be then spaced same time sampling measurement
Remaining enzyme activity, the reaction system (200 μ l) of detection are as follows: 100mMTris-HCl buffer solution (pH 8.5), 2.5mM pyruvic acid and
2.5mM (S)-Alpha-Methyl benzylamine, 3.92 × 10-7G pure protein (i.e. recombination ω-transaminase ATA-W12), is surveyed under 245nm wavelength
Determine absorption value, react 5min at 40 DEG C respectively, measurement result is as shown in fig. 7, show: at 40 DEG C, 168h enzyme activity not anti-reflection increases；
At 50 DEG C, 68h enzyme activity drops to 34.46%, 168h and is down to 5.54%；3h complete deactivation at 60 DEG C, 1h just loses completely at 70 DEG C
It is living.
In conclusion the ω-transaminase ATA-W12 has excellent zymologic property, catalytic temperature range is 20-60 DEG C,
Catalysis reduction pH value is 8.0-10.0.
8. recombinating the experiment of ω-transaminase ATA-W12 substrate spectrum analysis
The purpose of the experiment is detected to active according to the ω obtained of above-described embodiment 4-transaminase ATA-W12
Substrate.The reaction system (200 μ l) of detection are as follows: 100mMTris-HCl buffer solution (pH8.5), 2.5mM amino acceptor and
2.5mM (S)-Alpha-Methyl benzylamine, 3.92 × 10-7G pure protein (i.e. recombination ω-transaminase ATA-W12), is surveyed under 245nm wavelength
Determine absorption value, react 5min at 40 DEG C respectively, measurement result is as shown in table 1, and the enzyme activity of amino acceptor pyruvic acid is made in mode
It is 100%.ω-transaminase ATA-W12 is recombinated to the active highest of pyruvic acid, there is preferable activity to ketone ester substance,
In, higher to the vigor of methyl pyruvate and ethyl pyruvate, the opposite enzyme activity of methyl pyruvate reaches 94.6%, pyruvic acid second
The relative activity of ester is up to 94.09%, and lower to aromatic ketone and alkanones species activity.
Table 1
9. ω-transaminase ATA-W12 catalysis generates (S)-N-Boc-3- amino piperidine
By above-described embodiment 3 obtain containing obtained by ω-aminotransferase gene ata-w12 recombination engineered strain culture
Wet thallus ATA-W12 carries out automatic sample as catalyst in the three-necked flask of 100mL, and 2M isopropylamine solution controls pH
8.5.Reaction system (50mL): pH 8.5Tris-HCl, ATA-W12 wet thallus (1g/10mL), 500mM N-Boc-3- piperidines
Ketone, 2mL DMSO, 1.5M acidification isopropylamine, 0.2mM PLP are stirred in 40 DEG C of water-baths with the speed of 300rpm/min, every
1h sampling, takes Duplicate Samples three times, with high performance liquid chromatography detection (ZORBAX Extend-C18 column, 4.6 × 250mm × 5 μm, stream
Dynamic phase: 28% acetonitrile: 72% water (0.1%TFA), flow velocity 0.8mL/min, 37 DEG C of column temperature, 5 μ L, UV 210nm of sample volume detection,
Substrate N-Boc-3- piperidones appearance time 13min, product N-Boc-3- amino piperidine appearance time 3.8min), reaction process
Curve is as shown in Figure 8.500mM N-Boc-3- piperidones is catalyzed 8h by ω-transaminase ATA-W12 and obtains (S)-N-Boc-3-
Amino piperidine, conversion ratio 100%.After reaction, it takes sample to be extracted twice with the ethyl acetate of pentaploid product and adds anhydrous sulphur
Sour sodium is dried overnight, after be dissolved in isopropanol, utilize high performance liquid chromatography (Chiralcel AD-H, mobile phase: n-hexane/second
Alcohol=90:10v/v, 1mL/min, 30 DEG C of column temperature, UV 210nm detection) measure ee value > 99.95%.Enantiomeric excess value: ee
(%)=(R-S)/(R+S) × 100%, wherein R, S are respectively two kinds of Enantiomeric excess.Conversion ratio (C)=(product amount/substrate
Amount) × 100%.Reaction solution three times, merges extraction phase with isometric ethyl acetate extraction, and 12000rpm/min is centrifuged 10min,
30 DEG C of vacuum distillation recycling ethyl acetate of organic phase are taken, obtaining pale yellow oily liquid is the thick of (S)-N-Boc-3- amino piperidine
Product.
Above-described, only presently preferred embodiments of the present invention, the range being not intended to limit the invention, of the invention is upper
Stating embodiment can also make a variety of changes.Made by i.e. all claims applied according to the present invention and description
Simply, equivalent changes and modifications fall within the claims of the invention patent.The not detailed description of the present invention is
Routine techniques content.
Sequence table
<110>East China University of Science
<120>a kind of ω of S type-transaminase ATA-W12 and its gene and application
<160> 4
<210> 1
<211> 1407
<212> DNA
<213>cotton field soil metagenome
<220>
<221> gene
<222>
<223>ω-transaminase ATA-W12 gene
<400> 1
atgaccgccc ccctccgcaa ccacgacatc gccgagctca agcgcctgga cctggcccac 60
cacctgccgg cccaggccga tcacaaggtc atcgccgagc agggcggaag ccggatcatc 120
acccgcgccg agggcgtcta catccatgac ggcgagggcc atcagatcct cgacggcatg 180
gccgggctgt ggtgcgtgaa cgtcggctac ggtcgcgagg aactggccaa ggccgcctac 240
gaccagatgc tggagctgcc ctactacaat acgttcttca agaccgcgac gccgccgccg 300
atcgagctgg cggccaagat cgcgcagaag atgggcgggc atctttccca cgtcttctac 360
aattcgtcgg ggtcggaggc gaacgacacg gtcttccgcc tggtgcgcca cttttggaag 420
ttgaaggggg agccaagccg cacggtcttc atcagccgct ggaacgccta tcacggctcg 480
acggtggcgg gcgtcagcct gggcggcatg aagcacatgc acaagcaggg cgacctgccg 540
atcgccggcg tcgagcatgt gatgcagccc taccagttcg gcgacggctt cggcgaggat 600
ccggccgcct tccgcgaccg ggcggtgcag gccatcgagg acaagatcct ggaagtcggg 660
cccgagaacg tcgcggcctt catcggcgag ccggtgcagg gcgcgggcgg ggtgatcatc 720
ccgccggacg gctattggcc ggcggtcgag gccctgtgcc gcaagtacgg catcctgctg 780
gtctgcgacg aggtgatctg cggctttggg cggctgggcc agtggttcgg ccaccagcac 840
tatggcatca agcccgacct gatcgccatg gccaagggcc tgtcgtccgg ctatctgccg 900
atcagcgccg tgggcgtggc cgaccacatc gtcgccgagc tgcgcgagaa gggcggcgac 960
ttcatccacg gctttacgta ctcgggccac ccgacggcgg cggccgtggc gctgaagaac 1020
atcgagatca tggagcgcga gggcctggtc gagcgcaccc gcgacgagac cggcccctat 1080
ctggcgcagg ccctggccag cctcaacgac cacccgctgg tgggtgaagt tcgctcgctg 1140
ggcctgatcg gcgcggtcga gatcgtgcgc gagaagggga ccaaccaccg cttcctcgac 1200
aaggagggcg aggccgggcc gatcgtgcgc gacctgtgca tcaagaacgg cctgatggtt 1260
cgcgccatcc gcgacagcat cgtctgctgc ccgccgctga tcatcaccaa ggcgcagatc 1320
gacgagctgg tcggcatcat ccgaaagtcg ctcgacgaag ccgagccggt gctgcgggcg 1380
ctgaagccta aggagggcga ggactga 1407
<210> 2
<211> 468
<212> PRT
<213>cotton field soil metagenome
<400> 2
MTAPLRNHDIAELKRLDLAHHLPAQADHKVIAEQGGSRIITRAEGVYIHDGEGHQILDGMAGLWCVNVG
YGREELAKAAYDQMLELPYYNTFFKTATPPPIELAAKIAQKMGGHLSHVFYNSSGSEANDTVFRLVRHFWKLKGEPS
RTVFISRWNAYHGSTVAGVSLGGMKHMHKQGDLPIAGVEHVMQPYQFGDGFGEDPAAFRDRAVQAIEDKILEVGPEN
VAAFIGEPVQGAGGVIIPPDGYWPAVEALCRKYGILLVCDEVICGFGRLGQWFGHQHYGIKPDLIAMAKGLSSGYLP
ISAVGVADHIVAELREKGGDFIHGFTYSGHPTAAAVALKNIEIMEREGLVERTRDETGPYLAQALASLNDHPLVGEV
RSLGLIGAVEIVREKGTNHRFLDKEGEAGPIVRDLCIKNGLMVRAIRDSIVCCPPLIITKAQIDELVGIIRKSLDEA
EPVLRALKPKEGED
<210> 3
<211> 30
<212> DNA
<213>artificial sequence
<220>
<223>Shanghai Jierui Biology Engineering Co., Ltd
<400> 3
aaccggaatt catgaccgcc cccctccgca 30
<210> 4
<211> 30
<212> DNA
<213>artificial sequence
<220>
<223>Shanghai Jierui Biology Engineering Co., Ltd
<400> 4
aacccaagct ttcagtcctc gccctcctta 30
Claims (9)
1. a kind of ω of S type-transaminase ATA-W12, which is characterized in that its amino acid sequence is as shown in SEQ ID NO:2.
2. a kind of ω of S type-aminotransferase gene ata-w12, which is characterized in that its base sequence is as shown in SEQ ID NO:1.
3. a kind of recombinant plasmid, which is characterized in that the recombinant plasmid by S type according to claim 2 ω-transaminase
Gene ata-w12 connect built-up with expression vector plasmid.
4. a kind of ω of S type-transaminase ATA-W12 engineering strain, which is characterized in that the ω of the S type-transaminase ATA-
W12 engineering strain is the engineering strain for obtaining recombinant plasmid transformed host strain according to claim 3.
5. ω-transaminase ATA-W12 and the S type according to claim 4 of a kind of S type according to claim 1
Application of the ω-transaminase ATA-W12 engineering strain in synthesis of chiral amine product, wherein when amino group donor is
(S)-Alpha-Methyl benzylamine when, amino acceptor be selected from pyruvic acid, 3- trimethyl pyruvic acid, methyl pyruvate, ethyl pyruvate, 3- bromine
Prephenic acid ethyl ester, 4- chloro methyl acetoacetate, 4- chloroacetyl acetacetic ester, 4,4,4-3- acetyl fluoride ethyl acetate, propionyl second
Acetoacetic ester, 2- methyl-ethyl acetoacetate, 2- ethyl acetoacetic ester, 2- chloroacetyl acetacetic ester, HPA, propionic aldehyde, hexanal,
Aldehyde C-9, glyoxalic acid, α-ketoglutaric acid, N-Boc-3- pyrrolidones, N-Boc-3- piperidones, benzylacetone, methyln-hexyl ketone, acetone,
3-hydroxy-2-butanone, dihydroxyacetone or 4- hydroxy-2-butanone；When amino group donor is isopropylamine, amino acceptor includes N-Boc-3- piperidines
Ketone.
6. application according to claim 5, which is characterized in that the ω of the S type-transaminase ATA-W12 catalytic temperature
It is 20~60 DEG C.
7. application according to claim 5, which is characterized in that the ω of the S type-transaminase ATA-W12 catalysis reduction
PH is 8.0~10.0.
8. application according to claim 5, which is characterized in that the ω of the S type-transaminase ATA-W12 and the base
Because engineered strain be used to be catalyzed the ketogenesis of N-Boc-3- piperidines (S)-N-Boc-3- amino piperidine.
9. application according to claim 8 characterized by comprising by the ω of S type-transaminase ATA-W12 genetic engineering
The resulting ATA-W12 wet thallus of strain culturing carries out catalysis reaction: pH in 50mL reaction system below as catalyst
8.5 Tris-HCl, ATA-W12 wet thallus, 500mM N-Boc-3- piperidones, 2mL DMSO, 1.5M are acidified isopropylamine, 0.2mM
PLP is in 40 DEG C of water-baths, and stirring, reaction terminates, and reaction solution is extracted with ethyl acetate, and collects extraction phase, and centrifugation takes organic phase
Vacuum rotary steam recycles ethyl acetate, obtains the crude product that pale yellow oily liquid is (S)-N-Boc-3- amino piperidine.
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| CN109486784B (en) * | 2018-11-30 | 2020-06-09 | 江南大学 | Omega-transaminase mutant capable of catalyzing sitafloxacin five-membered ring key intermediate |
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| CN111647634A (en) * | 2020-07-09 | 2020-09-11 | 华东理工大学 | Method for asymmetric synthesis of (S) -1-Boc-3-aminopiperidine by continuous flow of packed bed |
| CN112481229B (en) * | 2020-11-25 | 2022-12-30 | 华东理工大学 | Omega transaminase and mutant, recombinant plasmid, genetic engineering bacteria and application thereof |
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