CN106520660A - Method for measuring pollen viability of bougainvillea spectabilis willd - Google Patents
Method for measuring pollen viability of bougainvillea spectabilis willd Download PDFInfo
- Publication number
- CN106520660A CN106520660A CN201610884890.6A CN201610884890A CN106520660A CN 106520660 A CN106520660 A CN 106520660A CN 201610884890 A CN201610884890 A CN 201610884890A CN 106520660 A CN106520660 A CN 106520660A
- Authority
- CN
- China
- Prior art keywords
- pollen
- bougainvillea
- filter paper
- fluid nutrient
- nutrient medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5097—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving plant cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for measuring pollen viability of bougainvillea spectabilis willd. The method comprises the following steps of 1, preparing a liquid culture medium: preparing the aqueous solution-based liquid culture medium containing 20-30g/L of sucrose, 15-25mg/L of boric acid, 20-40mg/L of Ca(NO3)2 and 2-4mg/L of GA3; 2, collecting pollen; 3, performing pollen culture; 4, observing a count; and 5, calculating a pollen germination rate, wherein the germination rate equals a germinated pollen particle number divided by a total pollen particle number multiplied by 100%. The technical scheme has the following beneficial effects: the pollen of the bougainvillea spectabilis willd is subjected to in vitro germination culture by using the liquid culture medium, so that the pollen viability can be observed more intuitively and accurately and the obtained data is more reliable. Through the method, it is discovered that the proper addition of plant hormones and nutritional elements during pollen viability measurement better facilitates pollen germination.
Description
Technical field
The invention belongs to plant technology field, more particularly to a kind of method for determining bougainvillea Pollen Activity.
Background technology
Bougainvillea（Bougainvillea spectabilia Willa）It is that Nyctaginaceae bougainvillea is evergreen to climb up by holding on to or drape over one's shoulders
Scattered shrub, originates in Brazil, also known as precious applique, Bougainvillea spectabilis, and bougainvillea is numerous in variety, and strong adaptability is easily cultivated, can floral leaf it is all
Reward, and pattern is more, the florescence is long, with important afforestation, environmental protection and medical value, wide market.The whole world
The original seed of bougainvillea, cultigen, mutation, cenospecies quantity have as many as 300 kinds.Bougainvillea modes of reproduction is mainly cuttage, grafting
Deng vegetative propagation, rearing new variety is mainly by screening mutant.As bougainvillea pollen viability is low, aborted embryo phenomenon is obvious,
Cause setting percentage low, the increase of generative propagation difficulty cultivates new varieties by sexual hybridization technology difficult.
It is because the size of Pollen Activity directly affects pollination, fertilization process, closely related with the acquisition of new variety of plant, lead to
Cross the measure of Pollen Activity, it may be appreciated that the fertility of pollen.At present, the research about bougainvillea Pollen Activity measure is little, though
Have a document report, but its primary study be the impact of sucrose and boric acid to bougainvillea Pollen Activity, be not directed to hormone and its
His impact of the nutrient to its vigor, and culture medium be solid medium；Flower is determined with TTC methods, IKI method
Can, although operation is simple for powder vigor, TTC methods can only identify the power of Pollen Activity, sprout unclear；Iodo- iodine
Potassium method is for not amyloid pollen and does not apply to for change；In liquid medium within of the present invention, cultured in vitro bougainvillea pollen, passes through
Bougainvillea Pollen Activity is determined, it is found that germination rate is up to 25%, therefore, great-hearted kind can be quickly selected as Parent
Cross experiment is carried out, can not only mitigate workload, breeding cycle can also be shortened, breeding efficiency is improved.
The content of the invention
Based on above the deficiencies in the prior art, technical problem solved by the invention is to provide a kind of measure bougainvillea
The method of powder vigor, determines the Pollen Activity of different cultivars bougainvillea using germination in vitro method, with TTC methods, IKI method phase
Than this kind of method is more accurate, reliable.
In order to solve above-mentioned technical problem, the present invention provides a kind of method for determining bougainvillea Pollen Activity, including as follows
Step：
Step one, configuration fluid nutrient medium：Prepare sucrose 20-30g/L, boric acid 15-25mg/L, Ca (NO3)220-40mg/L、
GA3The aqueous liquid culture medium of 2-4mg/L, the bottle after the culture medium for preparing is sterilized are installed to be put in 4 °C of refrigerators and are protected
Deposit；
Step 2, collection pollen：The flower of whole bougainvillea is plucked, three flower pesticide of bougainvillea is all cut with scalpel,
It is layered on clean filter paper；
Step 3, pollen cultures：The groove of slide is dropped in fluid nutrient medium obtained in a small amount of step one of glue head dropper suction
On, pollen is exposed in flower pesticide strip off obtained in step 2 with dissecting needle then, the groove of the flower pesticide in slide of pollen will be exposed
On fluid nutrient medium on dip in it is several under, pollen is fallen in fluid nutrient medium, spread two layers of filter paper in culture dish, use distilled water
Filter paper is drenched, polliniferous slide will be put and be placed in culture dish and be placed on the filter paper for drenching, be subsequently placed in incubated
Light culture 3-5 hours in case；
Step 4, observation are counted：Polliniferous slide of putting after step 3 is processed takes out, and puts under the microscope with 10 times of things
Sem observation, respectively process counterpoise is multiple 3 times, takes its mean value, and each processes 3 visuals field of observation, and each visual field counts pollen grain number not
Less than 30；
Step 5, pollen germination rate are calculated：The pollen grain number of germination rate=sprouted/pollen grain sum × 100%.
As the preferred embodiment of above-mentioned technical proposal, measure bougainvillea Pollen Activity provided in an embodiment of the present invention
Method further includes the part or all of of following technical characteristic：
Used as the improvement of above-mentioned technical proposal, in one embodiment of the invention, the time for collecting pollen in step 2 is chosen
Bougainvillea opens 8 points to 9 points of morning on the same day completely.
Used as the improvement of above-mentioned technical proposal, in one embodiment of the invention, the flower pesticide after processing in step 2 is put
Store under the conditions of -5-5 DEG C in drier.
As the improvement of above-mentioned technical proposal, in one embodiment of the invention, in step 3, the training of constant incubator
Foster temperature is 25-30 °C.
As the improvement of above-mentioned technical proposal, in one embodiment of the invention, in step 5, pollen germination standard is
Diameter of the pollen tube length more than or equal to germinal aperature.
Compared with prior art, technical scheme has the advantages that：
1. germination in vitro culture is carried out to bougainvillea pollen with fluid nutrient medium, can more intuitive and accurate observation pollen work
Power, the data of acquisition are relatively reliable.
2. found by the method, when Pollen Activity is determined, be properly added plant hormone and nutrient is more beneficial for
The sprouting of pollen.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, and in order to allow the present invention above and other objects, features and advantages can
Become apparent, below in conjunction with preferred embodiment, describe in detail as follows.
Specific embodiment
The specific embodiment of the present invention is described in detail with reference to embodiment, its part as this specification is led to
The principle of the embodiment explanation present invention is crossed, other aspects of the present invention, feature and its advantage will become by the detailed description
It is very clear.
A kind of method for determining bougainvillea Pollen Activity, it is characterised in that comprise the steps：
Step one, configuration fluid nutrient medium：Prepare sucrose 20-30g/L, boric acid 15-25mg/L, Ca (NO3)220-40mg/L、
GA3The aqueous liquid culture medium of 2-4mg/L, the bottle after the culture medium for preparing is sterilized are installed to be put in 4 °C of refrigerators and are protected
Deposit；
Step 2, collection pollen：The flower of whole bougainvillea is plucked, three flower pesticide of bougainvillea is all cut with scalpel,
It is layered on clean filter paper；
Step 3, pollen cultures：The groove of slide is dropped in fluid nutrient medium obtained in a small amount of step one of glue head dropper suction
On, pollen is exposed in flower pesticide strip off obtained in step 2 with dissecting needle then, the groove of the flower pesticide in slide of pollen will be exposed
On fluid nutrient medium on dip in it is several under, pollen is fallen in fluid nutrient medium, spread two layers of filter paper in culture dish, use distilled water
Filter paper is drenched, polliniferous slide will be put and be placed in culture dish and be placed on the filter paper for drenching, be subsequently placed in incubated
Light culture 3-5 hours in case；
Step 4, observation are counted：Polliniferous slide of putting after step 3 is processed takes out, and puts under the microscope with 10 times of things
Sem observation, respectively process counterpoise is multiple 3 times, takes its mean value, and each processes 3 visuals field of observation, and each visual field counts pollen grain number not
Less than 30；
Step 5, pollen germination rate are calculated：The pollen grain number of germination rate=sprouted/pollen grain sum × 100%.
The time selection bougainvillea for collecting pollen in step 2 opens 8 points to 9 points of morning on the same day completely.
Flower pesticide after processing in step 2 is placed in drier and stores under the conditions of -5-5 DEG C.
In step 3, the cultivation temperature of constant incubator is 25-30 °C.
In step 5, pollen germination standard is diameter of the pollen tube length more than or equal to germinal aperature.
Embodiment 1
Bougainvillea pollen viability measuring method of the present invention, comprises the steps：
Step one, configuration fluid nutrient medium, fluid nutrient medium include following components：Sucrose 20g/L, boric acid 25mg/L, Ca
(NO3)220mg/L, GA32mg/L, the culture medium bottle for preparing are installed to be put in 4 °C of refrigerators and are preserved.
Step 2, collection pollen：The whole flower of 8 points of harvestings of morning on the same day is opened completely in bougainvillea, is brought laboratory into, is used
Scalpel all cuts per colored three flower pesticide, is layered on clean filter paper.
Step 3, pollen cultures：A small amount of fluid nutrient medium is sucked with glue head dropper to drop on the groove of slide, Ran Houyong
Flower pesticide strip off is exposed pollen by dissecting needle, dip on culture medium it is several under（Bougainvillea pollen not only less but also difficult took out）, fall into pollen
In fluid nutrient medium, two layers of filter paper is spread in culture dish, filter paper is drenched with distilled water, will put polliniferous slide and be put into
On the filter paper for drenching, light culture in constant incubator is subsequently placed in, cultivation temperature is 25 °C.
Step 4, observation are counted：Pollen cultures were taken out after 3 hours, were put and were observed with 10 times of object lens under the microscope, each to process
It is repeated 3 times, takes its mean value, each processes 3 visuals field of observation, each visual field counts pollen grain 30.
Step 5, pollen germination rate are calculated：Pollen germination standard is diameter of the pollen tube length more than or equal to germinal aperature, is sprouted
Send out pollen grain number/pollen grain sum × 100% for rate=sprouted.
Embodiment 2
Step one, configuration fluid nutrient medium, fluid nutrient medium include following components：Sucrose 25g/L, boric acid 20mg/L, Ca
(NO3)230mg/L, GA33mg/L, the culture medium bottle for preparing are installed to be put in 4 °C of refrigerators and are preserved.
Step 2, collection pollen：The whole flower of morning on the same day 8 thirty harvesting is opened completely in bougainvillea, brings laboratory into,
Per colored three flower pesticide is all cut with scalpel, be layered on clean filter paper.
Step 3, pollen cultures：A small amount of fluid nutrient medium is sucked with glue head dropper to drop on the groove of slide, Ran Houyong
Flower pesticide strip off is exposed pollen by dissecting needle, dip on culture medium it is several under（Bougainvillea pollen not only less but also difficult took out）, fall into pollen
In fluid nutrient medium, two layers of filter paper is spread in culture dish, filter paper is drenched with distilled water, will put polliniferous slide and be put into
On the filter paper for drenching, light culture in constant incubator is subsequently placed in, cultivation temperature is 28 °C.
Step 4, observation are counted：Pollen cultures were taken out after 4 hours, were put and were observed with 10 times of object lens under the microscope, each to process
It is repeated 3 times, takes its mean value, each processes 3 visuals field of observation, each visual field counts pollen grain 35.
Step 5, pollen germination rate are calculated：Pollen germination standard is diameter of the pollen tube length more than or equal to germinal aperature, is sprouted
Send out pollen grain number/pollen grain sum × 100% for rate=sprouted.
Embodiment 3
Step one, configuration fluid nutrient medium, fluid nutrient medium include following components：Sucrose 30g/L, boric acid 15mg/L, Ca
(NO3)240mg/L, GA34mg/L, the culture medium bottle for preparing are installed to be put in 4 °C of refrigerators and are preserved.
Step 2, collection pollen：The whole flower of 9 points of harvestings of morning on the same day is opened completely in bougainvillea, is brought laboratory into, is used
Scalpel all cuts per colored three flower pesticide, is layered on clean filter paper.
Step 3, pollen cultures：A small amount of fluid nutrient medium is sucked with glue head dropper to drop on the groove of slide, Ran Houyong
Flower pesticide strip off is exposed pollen by dissecting needle, dip on culture medium it is several under（Bougainvillea pollen not only less but also difficult took out）, fall into pollen
In fluid nutrient medium, two layers of filter paper is spread in culture dish, filter paper is drenched with distilled water, will put polliniferous slide and be put into
On the filter paper for drenching, light culture in constant incubator is subsequently placed in, cultivation temperature is 30 °C.
Step 4, observation are counted：Take out after pollen cultures 3-5 hours, put and observed with 10 times of object lens under the microscope, everywhere
Reason is repeated 3 times, and takes its mean value, and each processes 3 visuals field of observation, and each visual field counts pollen grain 40.
Step 5, pollen germination rate are calculated：Pollen germination standard is diameter of the pollen tube length more than or equal to germinal aperature, is sprouted
Send out pollen grain number/pollen grain sum × 100% for rate=sprouted.
Table one, the pollen germination rate of embodiment 1-3 bougainvillea
Compared with prior art, technical scheme has the advantages that：
1. germination in vitro culture is carried out to bougainvillea pollen with fluid nutrient medium, can more intuitive and accurate observation pollen work
Power, the data of acquisition are relatively reliable.
2. found by the method, when Pollen Activity is determined, be properly added plant hormone and nutrient is more beneficial for
The sprouting of pollen.
The above is the preferred embodiment of the present invention, can not limit certainly the right model of the present invention with this
Enclose, it is noted that for those skilled in the art, under the premise without departing from the principles of the invention, may be used also
To make some improvement and variation, these improve and variation is also considered as protection scope of the present invention.
Claims (5)
1. it is a kind of determine bougainvillea Pollen Activity method, it is characterised in that comprise the steps：
Step one, configuration fluid nutrient medium：Prepare sucrose 20-30g/L, boric acid 15-25mg/L, Ca (NO3) 220-40mg/L,
The aqueous liquid culture medium of GA32-4mg/L, the bottle after the culture medium for preparing is sterilized are installed to be put in 4 °C of refrigerators and are protected
Deposit；
Step 2, collection pollen：The flower of whole bougainvillea is plucked, three flower pesticide of bougainvillea is all cut with scalpel,
It is layered on clean filter paper；
Step 3, pollen cultures：The groove of slide is dropped in fluid nutrient medium obtained in a small amount of step one of glue head dropper suction
On, pollen is exposed in flower pesticide strip off obtained in step 2 with dissecting needle then, the groove of the flower pesticide in slide of pollen will be exposed
On fluid nutrient medium on dip in it is several under, pollen is fallen in fluid nutrient medium, spread two layers of filter paper in culture dish, use distilled water
Filter paper is drenched, polliniferous slide will be put and be placed in culture dish and be placed on the filter paper for drenching, be subsequently placed in incubated
Light culture 3-5 hours in case；
Step 4, observation are counted：Polliniferous slide of putting after step 3 is processed takes out, and puts under the microscope with 10 times of things
Sem observation, respectively process counterpoise is multiple 3 times, takes its mean value, and each processes 3 visuals field of observation, and each visual field counts pollen grain number not
Less than 30；
Step 5, pollen germination rate are calculated：The pollen grain number of germination rate=sprouted/pollen grain sum × 100%.
2. the method for determining bougainvillea Pollen Activity as claimed in claim 1, it is characterised in that：Pollen is collected in step 2
Time is chosen bougainvillea and opens 8 points to 9 points of morning on the same day completely.
3. the method for determining bougainvillea Pollen Activity as claimed in claim 1, it is characterised in that：Flower after processing in step 2
Medicine is placed in drier and stores under the conditions of -5-5 DEG C.
4. the method for determining bougainvillea Pollen Activity as claimed in claim 1, it is characterised in that：It is in step 3, incubated
The cultivation temperature of case is 25-30 °C.
5. the method for determining bougainvillea Pollen Activity as claimed in claim 1, it is characterised in that：In step 5, pollen germination
Standard is diameter of the pollen tube length more than or equal to germinal aperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610884890.6A CN106520660A (en) | 2016-10-11 | 2016-10-11 | Method for measuring pollen viability of bougainvillea spectabilis willd |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610884890.6A CN106520660A (en) | 2016-10-11 | 2016-10-11 | Method for measuring pollen viability of bougainvillea spectabilis willd |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106520660A true CN106520660A (en) | 2017-03-22 |
Family
ID=58331599
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610884890.6A Pending CN106520660A (en) | 2016-10-11 | 2016-10-11 | Method for measuring pollen viability of bougainvillea spectabilis willd |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106520660A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114667922A (en) * | 2022-03-11 | 2022-06-28 | 云南为君开园林工程有限公司 | Bougainvillea spectabilis crossbreeding method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876768A (en) * | 2012-09-20 | 2013-01-16 | 浙江大学 | Method for measuring pollen vitality of lycoris plants using in-vitro germination method |
| CN103451147A (en) * | 2013-09-17 | 2013-12-18 | 武汉市蔬菜科学研究所 | Lactuca sativa L. pollen in vitro germination culture medium and method for measuring pollen activity |
| CN104357532A (en) * | 2014-10-11 | 2015-02-18 | 河北双星种业有限公司 | Method for rapidly and effectively measuring melon pollen viability |
| CN104694455A (en) * | 2015-04-01 | 2015-06-10 | 哈尔滨工业大学 | Sugar beet pollen germination cultivation method |
-
2016
- 2016-10-11 CN CN201610884890.6A patent/CN106520660A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876768A (en) * | 2012-09-20 | 2013-01-16 | 浙江大学 | Method for measuring pollen vitality of lycoris plants using in-vitro germination method |
| CN103451147A (en) * | 2013-09-17 | 2013-12-18 | 武汉市蔬菜科学研究所 | Lactuca sativa L. pollen in vitro germination culture medium and method for measuring pollen activity |
| CN104357532A (en) * | 2014-10-11 | 2015-02-18 | 河北双星种业有限公司 | Method for rapidly and effectively measuring melon pollen viability |
| CN104694455A (en) * | 2015-04-01 | 2015-06-10 | 哈尔滨工业大学 | Sugar beet pollen germination cultivation method |
Non-Patent Citations (8)
| Title |
|---|
| 刘雪莲等: "不同培养基对紫丁香花粉离体萌发的影响", 《东北林业大学学报》 * |
| 周群等: "18个品种三角梅花粉育性与花粉形态的观察", 《江西农业学报》 * |
| 夏瑾华等: "硼酸与蔗糖对三角梅花粉生活力的影响", 《江苏农业科学》 * |
| 李春牛等: "叶子花花期调控研究进展", 《河南农业科学》 * |
| 李颖颖等: "硝酸钙对荔枝花粉萌发和花粉管生长的影响", 《热带作物学报》 * |
| 王媛等: "不同浓度赤霉素对地被菊‘紫重楼’开花特性的影响", 《中国农学通报》 * |
| 王昌陵等: "响应面法对大豆花粉离体萌发主要影响因子的优化", 《江苏农业科学》 * |
| 王昌陵等: "应用Plackett-Burman设计法筛选大豆花粉萌发的主要影响因素", 《大豆科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114667922A (en) * | 2022-03-11 | 2022-06-28 | 云南为君开园林工程有限公司 | Bougainvillea spectabilis crossbreeding method and application |
| CN114667922B (en) * | 2022-03-11 | 2023-01-13 | 云南为君开园林工程有限公司 | Bougainvillea spectabilis crossbreeding method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Eng et al. | Polyploidization using colchicine in horticultural plants: A review | |
| Sajjad et al. | Effect of colchicine on in vitro polyploidy induction in African marigold (Tagetes erecta) | |
| Pinto et al. | Ploidy stability of somatic embryogenesis-derived Passiflora cincinnata Mast. plants as assessed by flow cytometry | |
| Chen et al. | Ploidy doubling by in vitro culture of excised protocorms or protocorm-like bodies in Phalaenopsis species | |
| Cui et al. | In vitro induction of tetraploid Ziziphus jujuba Mill. var. spinosa plants from leaf explants | |
| Gallone et al. | Polyploid induction in vitro using colchicine and oryzalin on Hebe ‘Oratia Beauty’: Production and characterization of the vegetative traits | |
| Kanchanapoom et al. | In vitro induction of tetraploid plants from callus cultures of diploid bananas (Musa acuminata, AA group)‘Kluai Leb Mu Nang’and ‘Kluai Sa’ | |
| Takahashi et al. | Effects of genotypes and culture conditions on microspore embryogenesis and plant regeneration in several subspecies of Brassica rapa L. | |
| Cai et al. | Induction, regeneration and characterization of tetraploids and variants in ‘Tapestry’caladium | |
| Chen et al. | Recent advances in anther culture of Hevea brasiliensis (Muell.-Arg.) | |
| Domblides et al. | Production of doubled haploid plants of Cucurbitaceae family crops through unpollinated ovule culture in vitro | |
| Hashim et al. | Oil palm (Elaeis guineensis Jacq.) somatic embryogenesis | |
| CN102972296B (en) | Acacia rachii pollen in-vitro culture activity detection technology | |
| Głowacka et al. | Impact of colchicine application during callus induction and shoot regeneration on micropropagation and polyploidisation rates in two Miscanthus species | |
| Gu et al. | Initiation and development of microspore embryogenesis and plant regeneration of Brassica nigra | |
| Caglar et al. | Progress in the production of haploid embryos, plants and doubled haploids in cucumber (C. sativus L.) by gamma irradiated pollen, in Turkey | |
| CN106879445B (en) | A kind of method of Rapid identification and screening sorghum germination period salt tolerance material | |
| CN106520660A (en) | Method for measuring pollen viability of bougainvillea spectabilis willd | |
| CN1799332A (en) | Method for preparing plant haploid embryo and haploid plant | |
| Yenchon et al. | Polyploidy induction of Dendrobium formosum by colchicine treatment in vitro | |
| Wongprichachan et al. | Induction of Polyploid Phalaenopsis amabilis by N_2O Treatment | |
| CN114766353A (en) | Rapid screening method and application of male pollination plants in actinidia arguta orchard | |
| Güneş et al. | Determination of pollen quantity, pollen viability and pollen germination in some dogrose species (Rosa section Caninae) | |
| Temjensangba et al. | Regeneration and mass multiplication of Arachnis labrosa (Lindl. ex Paxt.) Reichb: a rare and threatened orchid | |
| CN107155874A (en) | A kind of utilization Anther Culture obtains the method and its culture medium of Dihaploid Potato plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170322 |
|
| RJ01 | Rejection of invention patent application after publication |