CN106520560A - Isochrysis galbana 3010 culture medium composition - Google Patents
Isochrysis galbana 3010 culture medium composition Download PDFInfo
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- CN106520560A CN106520560A CN201611261840.9A CN201611261840A CN106520560A CN 106520560 A CN106520560 A CN 106520560A CN 201611261840 A CN201611261840 A CN 201611261840A CN 106520560 A CN106520560 A CN 106520560A
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- chrysophyceae
- culture medium
- isochrysis galbana
- culture
- medium composition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The invention discloses an isochrysis galbana 3010 culture medium composition. The isochrysis galbana 3010 culture medium composition comprises the following components: 75mg/L of NaNO3, 5mg/L of NaH2PO4, 3.16mg/L of FeCl3.6H2O, 4.36mg/L of Na2EDTA, 0.23g/L of ZnSO4.7H2O, 0.18g/L of MnCl2.4H2O, 0.01g/L of CuSO4.5H2O, 0.007g/L of Na2MoO4.2H2O, 0.12g/L of CoCl2.6H2O, an additive prepared from glucose and glycine, and a plant growth regulator prepared from gibberellins and 6-BA. The isochrysis galbana 3010 culture medium composition prepared with the technical scheme is adopted for performing culture discovery on isochrysis galbana 3010; and the isochrysis galbana 3010 has the characteristics of high growth rate and high cell density.
Description
Technical field
The present invention relates to culture medium technical field, more particularly to a kind of 3010 culture medium of chrysophyceae.
Background technology
Isochrysis galbana (Isochrysis galbana) is with individual little, acellular wall, digestible feature, rich in many
The high energy lipid material such as sugar, carrotene, is the bait micro-algae commonly used in shellfish culture.Chrysophyceae has higher medicinal valency simultaneously
Value, its beta carotene and high unsaturated fatty acid have anti-aging, anti-stress and promote the functions such as nervous system development.In profit
There is important DEVELOPMENT PROSPECT in terms of anti-ageing bioactivator of waiting for a long time is produced with algae.However, with chlorella (Chlorella
Vulgaris) compare with spirulina (Spirulina platensis) etc., the equilateral chrysophyceae of ball lacks the culture medium of characteristic and strong
Contamination resistance, the speed of growth and culture density are also relatively low in addition, more difficult to its large-scale industrial production.
How application come the speed of growth and culture density for improving chrysophyceae 3010 by 3010 culture media composition of chrysophyceae
People is devoted to the direction studied always.
The content of the invention
In order to solve the deficiency of prior art presence, the present invention provides one kind can make chrysophyceae 3010 have growth rate
Hurry up, big 3010 culture media composition of chrysophyceae of cell density.
For achieving the above object, the technical solution adopted in the present invention is：A kind of 3010 culture media composition of chrysophyceae, including
Following component：NaNO3：75mg/L；NaH2PO4：5mg/L；FeCl3·6H2O：3.16mg/L；Na2EDTA：4.36mg/L；
ZnSO4·7H2O：0.23g/L；MnCl2·4H2O：0.18g/L；CuSO4·5H2O：0.01g/L；Na2MoO4·2H2O：
0.007g/L；CoCl2·6H2O：0.12g/L；Plant made by additive made by glucose and glycine and gibberellin and 6-BA
Thing growth regulator.
It is further to improve, the additive：Glucose：1-2g/L；Glycine：0.5-3g/L.
It is further to improve, the plant growth regulator：Gibberellin：0.1-1mg/L；6-BA：0.1-1mg/L.
3010 culture medium of chrysophyceae of the present invention is beneficial to frustule fast-growth, 3010 cell density of chrysophyceae turned out
Greatly, especially because with the addition of plant growth regulator：Gibberellin 0.1-1mg/L and 6-BA 0.1-1mg/L；Additive：Portugal
Grape sugar 1-2g/L and glycine 0.5-3g/L, formula are reasonable, promote the fast-growth breeding of chrysophyceae 3010, and algae cell density increases
Greatly, it is suitable to large-scale production.
Description of the drawings
Fig. 1 is the comparative test result figure of embodiments of the invention 1, embodiment 2, embodiment 3 and seawater f/2 culture mediums.
Specific embodiment
With reference to specific embodiment, 3010 culture media composition of chrysophyceae that the present invention is provided is described in detail.
Embodiment one
A kind of 3010 culture media composition of chrysophyceae, including following component：NaNO3：75mg/L；NaH2PO4：5mg/L；
FeCl3·6H2O：3.16mg/L；Na2EDTA：4.36mg/L；ZnSO4·7H2O：0.23g/L；MnCl2·4H2O：0.18g/
L；CuSO4·5H2O：0.01g/L；Na2MoO4·2H2O：0.007g/L；CoCl2·6H2O：0.12g/L；Glucose：2g/L；
Glycine：3g/L；Gibberellin：1mg/L；6-BA：1mg/L.
Seawater after being filtered with 1000ml configures 3010 culture media composition of chrysophyceae of said ratio, takes the logarithm growth period
Chrysophyceae 3010 be inoculated into the culture medium in cultivated, daily illumination 12 hours, intensity of illumination be about 50 μm of ol/ (m2 s),
Training case temperature control detects algae cell density daily at 25 DEG C.
Embodiment two
A kind of 3010 culture media composition of chrysophyceae, including following component：NaNO3：75mg/L；NaH2PO4：5mg/L；
FeCl3·6H2O：3.16mg/L；Na2EDTA：4.36mg/L；ZnSO4·7H2O：0.23g/L；MnCl2·4H2O：0.18g/
L；CuSO4·5H2O：0.01g/L；Na2MoO4·2H2O：0.007g/L；CoCl2·6H2O：0.12g/L；Glucose：1.5g/
L；Glycine：1.5g/L；Gibberellin：0.5mg/L；6-BA：0.5mg/L.
Seawater after being filtered with 1000ml configures 3010 culture media composition of chrysophyceae of said ratio, takes the logarithm growth period
Chrysophyceae 3010 be inoculated into the culture medium in cultivated, daily illumination 12 hours, intensity of illumination be about 50 μm of ol/ (m2 s),
Training case temperature control detects algae cell density daily at 25 DEG C.
Embodiment three
A kind of 3010 culture media composition of chrysophyceae, including following component：NaNO3：75mg/L；NaH2PO4：5mg/L；
FeCl3·6H2O：3.16mg/L；Na2EDTA：4.36mg/L；ZnSO4·7H2O：0.23g/L；MnCl2·4H2O：0.18g/
L；CuSO4·5H2O：0.01g/L；Na2MoO4·2H2O：0.007g/L；CoCl2·6H2O：0.12g/L；Glucose：1g/L；
Glycine：0.5g/L；Gibberellin：0.1mg/L；6-BA：0.1mg/L.
Using seawater f/2 culture mediums as control medium, the chrysophyceae 3010 in growth period of taking the logarithm is inoculated into the culture medium
In cultivated, daily illumination 12 hours, intensity of illumination be about 50 μm of ol/ (m2 s), training case temperature control at 25 DEG C, daily
Detection algae cell density.
Following table is：The cell concentration contrast table of the chrysophyceae 3010 of different culture media culture
Can be seen that by the experimental data of upper table can using f/2 culture mediums using culture medium ratio provided by the present invention
To make 3010 growth and breeding of chrysophyceae more quickly, from optimization wild Oryza species culture chrysophyceae 3010, its highest cell concentration is far high
It is in control f/2 culture mediums, about high by 22%.Thus illustrate that 3010 culture medium of chrysophyceae of present invention offer has and significantly cultivate excellent
Gesture, is conducive to the fast-growth of chrysophyceae 3010.
Schematically specific embodiment of the invention is the foregoing is only, the scope of the present invention is not limited to.It is any
Those skilled in the art, done equivalent variations and modification on the premise of the design without departing from the present invention and principle,
The scope of protection of the invention should be belonged to.
Claims (3)
1. 3010 culture media composition of a kind of chrysophyceae, it is characterised in that：Including following component：NaNO3：75mg/L；NaH2PO4：
5mg/L；FeCl3·6H2O：3.16mg/L；Na2EDTA：4.36mg/L；ZnSO4·7H2O：0.23g/L；MnCl2·4H2O：
0.18g/L；CuSO4·5H2O：0.01g/L；Na2MoO4·2H2O：0.007g/L；CoCl2·6H2O：0.12g/L；Glucose
With additive made by glycine and plant growth regulator made by gibberellin and 6-BA.
2. 3010 culture media composition of chrysophyceae according to claim 1, it is characterised in that：The additive：Glucose：1-
2g/L；Glycine：0.5-3g/L.
3. 3010 culture media composition of chrysophyceae according to claim 1, it is characterised in that：The plant growth regulator：
Gibberellin：0.1-1mg/L；6-BA：0.1-1mg/L.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611261840.9A CN106520560A (en) | 2016-12-30 | 2016-12-30 | Isochrysis galbana 3010 culture medium composition |
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| CN201611261840.9A CN106520560A (en) | 2016-12-30 | 2016-12-30 | Isochrysis galbana 3010 culture medium composition |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251359A (en) * | 2020-12-21 | 2021-01-22 | 中国科学院烟台海岸带研究所 | Isochrysis for seawater seedling or cultivation in summer and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102281756A (en) * | 2009-01-13 | 2011-12-14 | α-J研究有限合伙公司 | Use of plant growth regulators to enhance algae growth |
| CN103805515A (en) * | 2014-03-04 | 2014-05-21 | 临沂大学 | Culture medium and culture method for Dicrateria zhanjiangensis |
-
2016
- 2016-12-30 CN CN201611261840.9A patent/CN106520560A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102281756A (en) * | 2009-01-13 | 2011-12-14 | α-J研究有限合伙公司 | Use of plant growth regulators to enhance algae growth |
| CN103805515A (en) * | 2014-03-04 | 2014-05-21 | 临沂大学 | Culture medium and culture method for Dicrateria zhanjiangensis |
Non-Patent Citations (1)
| Title |
|---|
| 黄振华: "湛江等鞭金藻有机碳营养生态位研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251359A (en) * | 2020-12-21 | 2021-01-22 | 中国科学院烟台海岸带研究所 | Isochrysis for seawater seedling or cultivation in summer and application thereof |
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