CN106513069A - A micro-fluidic chip - Google Patents
A micro-fluidic chip Download PDFInfo
- Publication number
- CN106513069A CN106513069A CN201610980770.6A CN201610980770A CN106513069A CN 106513069 A CN106513069 A CN 106513069A CN 201610980770 A CN201610980770 A CN 201610980770A CN 106513069 A CN106513069 A CN 106513069A
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- CN
- China
- Prior art keywords
- array
- cuboid
- entrance
- sample
- outlet
- Prior art date
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
A micro-fluidic chip is formed by bonding a substrate and a glass sheet and comprises a sample inlet, a sample detecting unit and a sample outlet. The sample detecting unit comprises a detecting unit inlet, a detecting unit array segment, a detecting unit outlet and an array formed by a plurality of cuboids. The detecting unit inlet is formed by an inlet straight tube segment and an inlet horn-shaped segment. The detecting unit outlet is formed by an outlet straight tube segment and an outlet horn-shaped segment. According to the dimension of the detecting unit array segment, the length is not more than 2750 [mu]m and not less than 1000 [mu]m, the width is not more than 1500 [mu]m and not less than 1000 [mu]m and the height is not more than 50 [mu]m and not less than 30 [mu]m. The cuboid array is fixed in the detecting unit array segment. In the cuboid array, center lines of the cuboids in adjacent rows are parallel, the cuboids in adjacent rows are staggered, and center straight lines of the cuboids in adjacent lines are parallel. The detecting unit array segment can be included in the visual field of a minimum quadruple object lens, and is not liable to block, and a compound to be detected in a sample is liable to deposit.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip, particular with the micro-fluidic chip of detector unit.
Background technology
Micro-fluidic chip on market includes sample entrance port, pattern detection unit and sample exit port, pattern detection unit
Size typically will not be included by the visual field of four times of minimum object lens completely；Flow additionally, due to during sample Jing pattern detection units
Speed will not become mitigation pattern detection unit and be not provided with rectangular volume array, and the material to be checked in sample is not easy precipitation；Can also be due to
The height dimension of pattern detection unit is too small to cause pattern detection unit to block.
The content of the invention
It is an object of the invention to provide a kind of pattern detection unit can by the visual field of four times of minimum object lens include completely into
Go, be not easy the micro-fluidic chip that blocks and the material to be checked in sample is easily precipitated.
To achieve these goals, the technical scheme is that：
A kind of micro-fluidic chip, is made up of substrate and sheet glass bonding, is gone out including sample entrance port, pattern detection unit and sample
Mouthful, the sample entrance port is sequentially communicated with pattern detection unit, sample exit port, and its innovative point is：
A, the pattern detection unit are by detector unit entrance, array of detection units section, detector unit outlet and multiple cuboids
The array of composition is constituted；
B, the detector unit entrance are made up of entrance straight length and entrance shape section, and entrance straight length is connected with sample entrance port
Logical, the width of entrance straight length is 500 ± 10 μm, and entrance shape section is connected with array of detection units section, entrance shape section
Inclination angle be 45 ± 5 degree；
C, detector unit outlet are made up of outlet straight length and outlet loudspeaker shape section, and outlet straight length is connected with sample exit port
Logical, the width for exporting straight length is 500 ± 5 μm, and outlet loudspeaker shape section is connected with array of detection units section, outlet loudspeaker shape section
Inclination angle is 45 ± 5 degree；
, in cuboid, its size is for d, the array of detection units section：1000 μm≤length≤2750 μm, 1000 μm≤wide
≤ 1500 μm of degree, 30 μm≤height≤50 μm；
E, the array of the plurality of cuboid composition are fixed in array of detection units section, and the boundary line of rectangular volume array is in square
Shape, the centerline parallel of adjacent two row cuboid in rectangular volume array, adjacent two row cuboid are interspersed, and adjacent rows are rectangular
The central line of body is parallel.
Cuboid in the array of above-mentioned multiple cuboid compositions, its a length of 40 ± 5 μm, a width of 10 ± 5 μm, a height of 30
Between～50 μm；The minimum interval of the edge line of adjacent two row cuboid is 40 μm；The edge line of adjacent rows cuboid is located
The minimum interval of straight line is 15 μm.
Above-mentioned substrate adopts dimethyl silicone polymer substrate, the array of detection units section bottom of the pattern detection unit
Corresponding glass panel region is the sheet glass that Jing silanization treatments or Jing plasma oxygens are processed, the thickness of sheet glass≤
0.17mm。
In above-mentioned rectangular volume array, the length direction of each cuboid is parallel with the length direction of array of detection units section.
Benefit of the invention is that：1）, due to the size of array of detection units section be：1000 μm≤length≤2750 μm,
1000 μm≤width≤1500 μm, 30 μm≤height≤50 μm, thus can by the visual field of four times of minimum object lens include completely into
Go；2）, be made up of entrance straight length and entrance shape section due to the detector unit entrance, thus sample is by entrance shape
During section flow detection cell array section, flow velocity slows down, and in making sample, material to be checked is easily precipitated；3）, due to the detector unit
Rectangular volume array is fixed with array segment, the central line of adjacent two row cuboid is parallel, and rectangular volume array is interspersed, phase
The central line of adjacent two row cuboids is parallel, thus the material to be checked in sample is easily precipitated；4）, due to array of detection units
Section height dimension be：30 μm≤height≤50 μm, thus pattern detection unit is not easy blocking.
Description of the drawings
Below in conjunction with accompanying drawing and the embodiment for being given, the present invention is further illustrated.
Fig. 1 is the structural representation of the micro-fluidic chip of the present invention；
Fig. 2 is the enlarged diagram of pattern detection unit；
Fig. 3 is the schematic top plan view of pattern detection unit；
Fig. 4 is the schematic perspective view of pattern detection unit.
Specific embodiment
As shown in figures 1-4, a kind of micro-fluidic chip, is made up of substrate and sheet glass bonding, including sample entrance port 1, sample
Detector unit 2 and sample exit port 3, the sample entrance port 1 are sequentially communicated with pattern detection unit 2, sample exit port 3, its innovative point
It is：
The pattern detection unit 2 is by detector unit entrance 2-1, array of detection units section 2-2, detector unit outlet 2-3 and many
The array of individual cuboid 2-4 compositions is constituted；
The detector unit entrance 2-1 is made up of entrance straight length 2-1-1 and entrance shape section 2-1-2, entrance straight length 2-
1-1 is connected with sample entrance port 1, and the width of entrance straight length 2-1-1 is 500 ± 5 μm, and entrance shape section 2-1-2 is single with detection
Element array section 2-2 is connected, and the inclination angle of entrance shape section 2-1-2 is 45 ± 5 degree；
Detector unit outlet 2-3 exports straight length 2- by straight length 2-3-1 is exported and outlet loudspeaker shape section 2-3-2 is constituted
3-1 is connected with sample exit port 3, and the width for exporting straight length 2-3-1 is 500 ± 5 μm, and outlet loudspeaker shape section 2-3-2 is single with detection
Element array section 2-2 is connected, and the inclination angle of outlet loudspeaker shape section 2-3-2 is 45 ± 5 degree；
In cuboid, its size is array of detection units section 2-2：1000 μm≤length≤2750 μm, 1000 μm≤wide
≤ 1500 μm of degree, 30 μm≤height≤50 μm；
The array of the plurality of cuboid 2-4 compositions is fixed in array of detection units section 2-2, the border of cuboid 2-4 arrays
Line is rectangular, the centerline parallel of adjacent two row cuboid 2-4 in cuboid 2-4 arrays, and adjacent two row cuboid 2-4 staggeredly divides
Cloth, the central line of adjacent rows cuboid 2-4 are parallel.
Cuboid 2-4 in the array of above-mentioned multiple cuboid 2-4 compositions is its a length of 40 ± 5 μm, a width of 10 ± 5 μm, high
Between 30～50 μm；The minimum interval of the edge line of adjacent two row cuboid 2-4 is 40 μm；Adjacent rows cuboid 2-4
Edge line place straight line minimum interval be 15 μm.
Above-mentioned substrate adopts dimethyl silicone polymer substrate, array of detection units section 2-2 of the pattern detection unit 2
The corresponding glass panel region in bottom is the sheet glass that Jing silanization treatments or Jing plasma oxygens are processed, the thickness of sheet glass≤
0.17mm。
The length side of the length direction of each cuboid 2-4 and array of detection units section 2-2 in above-mentioned cuboid 2-4 arrays
To parallel.
The pattern detection unit that the micro-fluidic chip of the present invention is carried is as the size of array of detection units section can be minimum
The visual field of four times of object lens include completely；Detector unit entrance includes entrance straight length and entrance shape section, makes sample
Slowed down by the flow velocity of entrance shape section flow detection cell array section, and material to be checked is easily precipitated in sample；Detection is single
The cuboid array layout arranged in first chamber is suitable, thus the material to be checked in sample is easily precipitated；Detector unit chamber
Height dimension it is suitable, thus pattern detection unit is not easy blocking.Therefore be used to treat test sample using the micro-fluidic chip of the present invention
During this detection, the data for being obtained are more accurate.With practical application effect.
Claims (4)
1. a kind of micro-fluidic chip, is made up of substrate and sheet glass bonding, including sample entrance port（1）, pattern detection unit（2）With
Sample exit port（3）, the sample entrance port（1）With pattern detection unit（2）, sample exit port（3）It is sequentially communicated, it is characterised in that：
A, the pattern detection unit（2）By detector unit entrance（2-1）, array of detection units section（2-2）, detector unit outlet
（2-3）With multiple cuboids（2-4）The array of composition is constituted；
B, the detector unit entrance（2-1）By entrance straight length（2-1-1）With entrance shape section（2-1-2）Constitute, entrance
Straight length（2-1-1）With sample entrance port（1）Connection, entrance straight length（2-1-1）Width be 500 ± 10 μm, entrance shape
Section（2-1-2）With array of detection units section（2-2）Connection, entrance shape section（2-1-2）Inclination angle be 45 ± 5 degree；
C, detector unit outlet（2-3）By outlet straight length（2-3-1）With outlet loudspeaker shape section（2-3-2）Constitute, outlet
Straight length（2-3-1）With sample exit port（3）Connection, exports straight length（2-3-1）Width be 500 ± 10 μm, outlet loudspeaker shape
Section（2-3-2）With array of detection units section（2-2）Connection, outlet loudspeaker shape section（2-3-2）Inclination angle be 45 ± 5 degree；
D, the array of detection units section（2-2）In cuboid, its size is：1000 μm≤length≤2750 μm, 1000 μm
≤ width≤1500 μm, 30 μm≤height≤50 μm；
E, the plurality of cuboid（2-4）The array of composition is fixed on array of detection units section（2-2）In, cuboid（2-4）Battle array
The boundary line of row is rectangular, cuboid（2-4）Adjacent two row cuboid in array（2-4）Centerline parallel, it is adjacent two row length
Cube（2-4）It is interspersed, adjacent rows cuboid（2-4）Central line it is parallel.
2. micro-fluidic chip according to claim 1, it is characterised in that：The plurality of cuboid（2-4）The array of composition
In cuboid（2-4）, its a length of 40 ± 5 μm, a width of 10 ± 5 μm, between a height of 30～50 μm；Adjacent two row cuboid（2-
4）Edge line minimum interval be 40 μm；Adjacent rows cuboid（2-4）The minimum interval by edge line place straight line be
15μm。
3. micro-fluidic chip according to claim 1, it is characterised in that：The substrate adopts dimethyl silicone polymer base
Piece, the pattern detection unit（2）Array of detection units section（2-2）The corresponding glass panel region in bottom is at Jing silanizations
The sheet glass that reason or Jing plasma oxygens are processed, the thickness≤0.17mm of sheet glass.
4. micro-fluidic chip according to claim 1, it is characterised in that：The cuboid（2-4）In array, each is rectangular
Body（2-4）Length direction and array of detection units section（2-2）Length direction it is parallel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610980770.6A CN106513069A (en) | 2016-11-08 | 2016-11-08 | A micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610980770.6A CN106513069A (en) | 2016-11-08 | 2016-11-08 | A micro-fluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106513069A true CN106513069A (en) | 2017-03-22 |
Family
ID=58350192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610980770.6A Pending CN106513069A (en) | 2016-11-08 | 2016-11-08 | A micro-fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106513069A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018184382A1 (en) * | 2017-04-06 | 2018-10-11 | 美康生物科技股份有限公司 | Microfluidic chip for separating and detecting whole blood sample and detection method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101102847A (en) * | 2005-01-18 | 2008-01-09 | 生物概念股份有限公司 | Cell separation using microchannel having patterned posts |
| CN103341372A (en) * | 2013-07-05 | 2013-10-09 | 西北工业大学 | Micro-fluidic chip structure for flow cytometer, and preparation method of micro-fluidic chip |
| CN105381824A (en) * | 2014-08-26 | 2016-03-09 | 中央研究院 | Collector architecture layout design |
| CN105848782A (en) * | 2013-10-25 | 2016-08-10 | 三叶虫创新股份有限公司 | Fluid filtering device and assembly |
-
2016
- 2016-11-08 CN CN201610980770.6A patent/CN106513069A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101102847A (en) * | 2005-01-18 | 2008-01-09 | 生物概念股份有限公司 | Cell separation using microchannel having patterned posts |
| CN103341372A (en) * | 2013-07-05 | 2013-10-09 | 西北工业大学 | Micro-fluidic chip structure for flow cytometer, and preparation method of micro-fluidic chip |
| CN105848782A (en) * | 2013-10-25 | 2016-08-10 | 三叶虫创新股份有限公司 | Fluid filtering device and assembly |
| CN105381824A (en) * | 2014-08-26 | 2016-03-09 | 中央研究院 | Collector architecture layout design |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018184382A1 (en) * | 2017-04-06 | 2018-10-11 | 美康生物科技股份有限公司 | Microfluidic chip for separating and detecting whole blood sample and detection method thereof |
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| C06 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180402 Address after: Tianning District 213000 Jiangsu city of Changzhou province Zheng Lu Feng River Road No. 8 Applicant after: Sharp microfluidic medical technology (Changzhou) Co., Ltd. Address before: Zheng Lu Tianning District 213115 Jiangsu city of Changzhou Province River Estuary three Feng Road No. 8 Applicant before: Changzhou Rui De medical science and technology company limited |
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| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170322 |
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| RJ01 | Rejection of invention patent application after publication |