CN106512080A - Preparation method for sericin protein hydrogel - Google Patents
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- CN106512080A CN106512080A CN201611044903.5A CN201611044903A CN106512080A CN 106512080 A CN106512080 A CN 106512080A CN 201611044903 A CN201611044903 A CN 201611044903A CN 106512080 A CN106512080 A CN 106512080A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0033—Collagen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0085—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/009—Materials resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
- A61L2300/604—Biodegradation
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Abstract
The invention provides a preparation method for sericin protein hydrogel. The preparation method for the sericin protein hydrogel includes the steps that firstly, silkworm cocoons are obtained, crushed, cleaned and air-dried, fragments are added into a water solution containing compound enzymes, high-pressure homogenization is carried out, and enzymatic hydrolysate is obtained; then, a micro-filtration membrane is used for filtering the enzymatic hydrolysate so as to remove impurities, obtained clear liquid is guided into a chromatographic column to conduct impurity removal, and a sericin protein solution is obtained; and finally, the obtained sericin protein solution is subjected to reduced pressure concentration until the volume is reduced to 15-20%, glutaraldehyde and citric acid are added, stirring and standing are carried out, and then the sericin protein hydrogel is obtained. According to the preparation method for the sericin protein hydrogel, sericin protein is extracted through the compound enzymes in combination with the process of high-pressure homogenization, the sericin protein extraction efficiency of the process can reach 90%, and the prepared sericin protein hydrogel not only has porosity and degradability but also has good biocompatibility.
Description
Technical field
The invention belongs to pharmaceuticss technical field, and in particular to a kind of preparation method of sericin hydrogel.
Background technology
Hydrogel（Hydrogel）It is the gel with water as disperse medium, the water soluble polymer with cross-linked network
It is middle to introduce a part of hydrophobic group and hydrophilic residue, hydrophilic residue and water molecules, hydrone is connected to into netted inside, and
The cross linked polymer of hydrophobic residue water-swellable.It is a kind of macromolecule network system, property is soft, can keep certain shape,
Substantial amounts of water can be absorbed.One class adopts natural biologic material（Such as collagen, alginate and shitosan etc.）The water-setting for preparing
Glue, because possessing the physicochemical property similar to body height and superior biology performance, has been widely used in biological doctor
Medicine and field of tissue engineering technology.
Sericin（SS）It is a kind of excellent natural macromolecular material of biocompatibility.Sericin rich in serine,
Aspartic acid, glutamic acid, threonine isopolarity aminoacid, account for the 70% of sericin total amino acid content, with human body skin angle
In matter layer, the aminoacid of nature moisturizing factor is constituted and is in similar proportion.As sericin has good biodegradability,
Promote the functional characteristics such as cell adhesion differentiation and wound healing, be a kind of excellent bio-medical material.
For a long time due to the people deficiency recognized to sericin and the limitation studied, cause sericin in filature industry
In be taken as waste to process, waste the natural resources of a large amount of preciousnesses.In recent years it is found that sericin has moisturizing, resists
Bacterium, antioxidation, anticoagulation and the promotion biological nature such as cell adhesion and propagation.
The content of the invention
The technical problem of solution：The purpose of the present invention is to overcome the deficiencies in the prior art and provide a kind of sericin water-setting
The preparation method of glue, made sericin hydrogel not only have porous, degradability, also with good biofacies
Capacitive.
Technical scheme：A kind of preparation method of sericin hydrogel, comprises the following steps：
Step 1, takes Bombyx bombycis and crushes, dry, obtain fragment after cleaning；
Step 2, fragment is added in the aqueous solution containing 1-4wt.% compound enzymes, and high pressure homogenize obtains enzymolysis solution；
Step 3, carries out filtering and impurity removing to enzymolysis solution using micro-filtration membrane, obtains clear liquid；
Step 4, clear liquid is imported in chromatographic column carries out remove impurity, obtains sericin solution；
Gained sericin solution decompression is concentrated into volume-diminished for 15-20% by step 5, add 0.5-1.2wt.% glutaraldehydes,
0.2-0.7wt.% citric acids, stirring stand, obtain final product.
Further, in step 1 chip size in 0.5-1cm.
Further, in step 2, compound enzyme is neutral protease and liquid cellulase by weight 2：The mixing of 3 compositions
Thing.
Further, step 2 mesohigh processing condition is 0.8-1.2MPa, circulation 5 times.
Further, in step 4, chromatographic column is polyamide column.
Further, also need to add 0.1-0.5wt.% sodium alginates in step 5.
Beneficial effect：The present invention extracts sericin, the technique sericin egg using the technique that compound enzyme combines high pressure homogenize
Up to 90%, made sericin hydrogel not only has porous, degradability to white extraction efficiency, also with good
Biocompatibility.
Specific embodiment
Embodiment 1
A kind of preparation method of sericin hydrogel, comprises the following steps：
Step 1, takes Bombyx bombycis and crushes, dry, obtain fragment after cleaning；
Step 2, fragment is added in the aqueous solution containing 1wt.% compound enzymes, and high pressure homogenize obtains enzymolysis solution；
Step 3, carries out filtering and impurity removing to enzymolysis solution using micro-filtration membrane, obtains clear liquid；
Step 4, clear liquid is imported in chromatographic column carries out remove impurity, obtains sericin solution；
Step 5, it is 15% that gained sericin solution decompression is concentrated into volume-diminished, adds 0.5wt.% glutaraldehydes, 0.2wt.%
Citric acid, stirring stand, obtain final product.
Wherein, in step 1 chip size in 0.5-1cm；In step 2, compound enzyme is neutral protease and liquid cellulase
By weight 2：The mixture of 3 compositions, high pressure homogenize condition are 0.8MPa, circulation 5 times；In step 4, chromatographic column is polyamide column.
Embodiment 2
A kind of preparation method of sericin hydrogel, comprises the following steps：
Step 1, takes Bombyx bombycis and crushes, dry, obtain fragment after cleaning；
Step 2, fragment is added in the aqueous solution containing 2wt.% compound enzymes, and high pressure homogenize obtains enzymolysis solution；
Step 3, carries out filtering and impurity removing to enzymolysis solution using micro-filtration membrane, obtains clear liquid；
Step 4, clear liquid is imported in chromatographic column carries out remove impurity, obtains sericin solution；
Step 5, it is 17% that gained sericin solution decompression is concentrated into volume-diminished, adds 0.6wt.% glutaraldehydes, 0.5wt.%
Citric acid, stirring stand, obtain final product.
Wherein, in step 1 chip size in 0.5-1cm；In step 2, compound enzyme is neutral protease and liquid cellulase
By weight 2：The mixture of 3 compositions, high pressure homogenize condition are 0.9MPa, circulation 5 times；In step 4, chromatographic column is polyamide column.
Embodiment 3
A kind of preparation method of sericin hydrogel, comprises the following steps：
Step 1, takes Bombyx bombycis and crushes, dry, obtain fragment after cleaning；
Step 2, fragment is added in the aqueous solution containing 1wt.% compound enzymes, and high pressure homogenize obtains enzymolysis solution；
Step 3, carries out filtering and impurity removing to enzymolysis solution using micro-filtration membrane, obtains clear liquid；
Step 4, clear liquid is imported in chromatographic column carries out remove impurity, obtains sericin solution；
Step 5, it is 18% that gained sericin solution decompression is concentrated into volume-diminished, adds 0.6wt.% glutaraldehydes, 0.4wt.%
Citric acid, stirring stand, obtain final product.
Wherein, in step 1 chip size in 0.5-1cm；In step 2, compound enzyme is neutral protease and liquid cellulase
By weight 2：The mixture of 3 compositions, high pressure homogenize condition are 1MPa, circulation 5 times；In step 4, chromatographic column is polyamide column.
Embodiment 4
A kind of preparation method of sericin hydrogel, comprises the following steps：
Step 1, takes Bombyx bombycis and crushes, dry, obtain fragment after cleaning；
Step 2, fragment is added in the aqueous solution containing 4wt.% compound enzymes, and high pressure homogenize obtains enzymolysis solution；
Step 3, carries out filtering and impurity removing to enzymolysis solution using micro-filtration membrane, obtains clear liquid；
Step 4, clear liquid is imported in chromatographic column carries out remove impurity, obtains sericin solution；
Step 5, it is 20% that gained sericin solution decompression is concentrated into volume-diminished, adds 1.2wt.% glutaraldehydes, 0.7wt.%
Citric acid, stirring stand, obtain final product.
Wherein, in step 1 chip size in 0.5-1cm；In step 2, compound enzyme is neutral protease and liquid cellulase
By weight 2：The mixture of 3 compositions, high pressure homogenize condition are 1.2MPa, circulation 5 times；In step 4, chromatographic column is polyamide column.
Embodiment 5
The present embodiment with the difference of embodiment 3 is：Also need to add 0.1-0.5wt.% sodium alginates in step 5.
A kind of preparation method of sericin hydrogel, comprises the following steps：
Step 1, takes Bombyx bombycis and crushes, dry, obtain fragment after cleaning；
Step 2, fragment is added in the aqueous solution containing 1wt.% compound enzymes, and high pressure homogenize obtains enzymolysis solution；
Step 3, carries out filtering and impurity removing to enzymolysis solution using micro-filtration membrane, obtains clear liquid；
Step 4, clear liquid is imported in chromatographic column carries out remove impurity, obtains sericin solution；
Step 5, it is 18% that gained sericin solution decompression is concentrated into volume-diminished, adds 0.6wt.% glutaraldehydes, 0.4wt.%
Citric acid, stirring stand, obtain final product.
Wherein, in step 1 chip size in 0.5-1cm；In step 2, compound enzyme is neutral protease and liquid cellulase
By weight 2：The mixture of 3 compositions, high pressure homogenize condition are 1MPa, circulation 5 times；In step 4, chromatographic column is polyamide column.
The present invention extracts sericin using the technique that compound enzyme combines high pressure homogenize, and the extraction efficiency of the technique sericin can
Up to 90%, made sericin hydrogel not only has porous, degradability, also with good biocompatibility.
Claims (6)
1. a kind of preparation method of sericin hydrogel, it is characterised in that：Comprise the following steps：
Step 1, takes Bombyx bombycis and crushes, dry, obtain fragment after cleaning；
Step 2, fragment is added in the aqueous solution containing 1-4wt.% compound enzymes, and high pressure homogenize obtains enzymolysis solution；
Step 3, carries out filtering and impurity removing to enzymolysis solution using micro-filtration membrane, obtains clear liquid；
Step 4, clear liquid is imported in chromatographic column carries out remove impurity, obtains sericin solution；
Gained sericin solution decompression is concentrated into volume-diminished for 15-20% by step 5, add 0.5-1.2wt.% glutaraldehydes,
0.2-0.7wt.% citric acids, stirring stand, obtain final product.
2. the preparation method of sericin hydrogel according to claim 1, it is characterised in that：Chip size in step 1
In 0.5-1cm.
3. the preparation method of sericin hydrogel according to claim 1, it is characterised in that：In step 2, compound enzyme is
Neutral protease and liquid cellulase by weight 2：The mixture of 3 compositions.
4. the preparation method of sericin hydrogel according to claim 1, it is characterised in that：Step 2 mesohigh homogenizing
Condition is 0.8-1.2MPa, circulation 5 times.
5. the preparation method of sericin hydrogel according to claim 1, it is characterised in that：In step 4, chromatographic column is
Polyamide column.
6. the preparation method of sericin hydrogel according to claim 1, it is characterised in that：Also need to add in step 5
Enter 0.1-0.5wt.% sodium alginates.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611044903.5A CN106512080A (en) | 2016-11-24 | 2016-11-24 | Preparation method for sericin protein hydrogel |
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| CN201611044903.5A CN106512080A (en) | 2016-11-24 | 2016-11-24 | Preparation method for sericin protein hydrogel |
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| CN106512080A true CN106512080A (en) | 2017-03-22 |
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| CN201611044903.5A Pending CN106512080A (en) | 2016-11-24 | 2016-11-24 | Preparation method for sericin protein hydrogel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115772376A (en) * | 2022-11-30 | 2023-03-10 | 浙江理工大学 | Preparation method of environment-friendly glue based on sericin-WPU-polyamide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228438A (en) * | 1998-12-01 | 1999-09-15 | 浙江丝绸工学院 | Sericin polypeptide extracted from frison, husks and waste silk and its extracting method |
| CN101810881A (en) * | 2010-04-27 | 2010-08-25 | 浙江大学 | Preparation method of sericin/hydroxyapatite composite porous bracket |
| CN103951831A (en) * | 2014-02-28 | 2014-07-30 | 华中科技大学同济医学院附属协和医院 | Preparation method and application of sericin hydrogel |
| CN105233336A (en) * | 2015-07-15 | 2016-01-13 | 华中科技大学同济医学院附属协和医院 | Sericin nerve guidance conduit as well as preparation method and application thereof |
-
2016
- 2016-11-24 CN CN201611044903.5A patent/CN106512080A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228438A (en) * | 1998-12-01 | 1999-09-15 | 浙江丝绸工学院 | Sericin polypeptide extracted from frison, husks and waste silk and its extracting method |
| CN101810881A (en) * | 2010-04-27 | 2010-08-25 | 浙江大学 | Preparation method of sericin/hydroxyapatite composite porous bracket |
| CN103951831A (en) * | 2014-02-28 | 2014-07-30 | 华中科技大学同济医学院附属协和医院 | Preparation method and application of sericin hydrogel |
| CN105233336A (en) * | 2015-07-15 | 2016-01-13 | 华中科技大学同济医学院附属协和医院 | Sericin nerve guidance conduit as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 叶崇军等: "全天然丝胶蛋白的提取及酶解条件的筛选", 《中国蚕业》 * |
| 张业顺: "丝胶蛋白水凝胶的制备及应用研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115772376A (en) * | 2022-11-30 | 2023-03-10 | 浙江理工大学 | Preparation method of environment-friendly glue based on sericin-WPU-polyamide |
| CN115772376B (en) * | 2022-11-30 | 2024-04-05 | 浙江理工大学 | Preparation method of environment-friendly glue based on sericin-WPU-polyamide |
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