CN106501439A - A kind of red ganoderma extract reference substance and its preparation method and application - Google Patents
A kind of red ganoderma extract reference substance and its preparation method and application Download PDFInfo
- Publication number
- CN106501439A CN106501439A CN201610821305.8A CN201610821305A CN106501439A CN 106501439 A CN106501439 A CN 106501439A CN 201610821305 A CN201610821305 A CN 201610821305A CN 106501439 A CN106501439 A CN 106501439A
- Authority
- CN
- China
- Prior art keywords
- red ganoderma
- acid
- red
- reference substance
- medicinal material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of red ganoderma extract reference substance and its preparation method and application, the preparation method of the red ganoderma extract reference substance includes the step of red ganoderma being extracted to obtain red ganoderma extract, also include the step of different red ganoderma extracts mutually being blent to obtain red ganoderma extract reference substance, each component containing following weight/mass percentage composition in the red ganoderma extract reference substance：Ganoderic acid A is 0.3% 0.5%, and ganoderic acid B is 0.15% 0.4%, and ganoderic acid C 2 is 0.3% 0.5%, and Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- is 0.25% 0.5%.The red ganoderma extract reference substance that the present invention is provided, can in effectively solving red ganoderma quality evaluation reference substance high cost, and cannot accurate evaluation red ganoderma quality comprehensively problem.
Description
Technical field
The invention belongs to glossy ganoderma Quality Control Technology field, more particularly to a kind of red ganoderma reference substance extract and its preparation
Methods and applications.
Background technology
History of the glossy ganoderma in the existing more than one thousand years of China, early in《Sheng Nong's herbal classic》In just on the books, glossy ganoderma reinvigoration, long
Food is not old, macrobiosis of making light of one's life by commiting suicide.
However, glossy ganoderma is used as a kind of Mycophyta medicinal material, various in style, the glossy ganoderma chemical component difference of different cultivars is huge,
Even if being all red ganoderma, it is divided into a lot of subspecies again, the different parts fructification of glossy ganoderma is also entirely different with spore meal component, mycelia
Body also has very big difference on composition with fructification.Effect of Chinese medicine is built upon the composition of the chemical substance contained by which and contains
On the basis of amount is stable, if chemical composition and content difference are very big, then its effect will necessarily produce very big uncertain
Property.Meanwhile, also cannot necessarily be accomplished with the ganoderma lucidum product that the huge glossy ganoderma medicinal material of chemical composition and content difference is produced as raw material
Steady quality reliability, determined curative effect.Therefore, glossy ganoderma medicinal material and ganoderma lucidum product are differentiated and quality evaluation is extremely important.
At present, the active ingredient of glossy ganoderma medicinal material is ganodenic acid and polysaccharide.Detection ganodenic acid main method is purple at present
Outer method, but the method specificity difference cannot be distinguished by ganodenic acid and sterol.There is scholar's liquid chromatography in the art, with glossy ganoderma
Middle different ganodenic acid monomer compound control product are reference, ganodenic acid content in detection glossy ganoderma medicinal material.This method exclusive
Property strong, but due to the excessively rare costliness of ganodenic acid reference substance, and a kind of content of single ganodenic acid monomer compound cannot
Represent the content of whole ganoderic acids in glossy ganoderma.Therefore the method is difficult to apply to production and the field of circulation.
Exploitation is a kind of effectively reduces cost and can be conducive to the reference substance of comprehensive accurate evaluation red ganoderma quality have very much must
Will.
Content of the invention
The present invention is to make up defect present in prior art, there is provided a kind of red ganoderma extract reference substance and red ganoderma are carried
Take the preparation method of thing reference substance, can in effectively solving red ganoderma quality evaluation reference substance high cost, and cannot be comprehensively accurate
Evaluate the good and bad problem of red ganoderma.
, for reaching its purpose, the technical scheme of employing is as follows for the present invention：
A kind of preparation method of red ganoderma extract reference substance, including being extracted to obtain red ganoderma extraction to red ganoderma
The step of thing, also include the step of different red ganoderma extracts mutually being blent to obtain red ganoderma extract reference substance, institute
State each component containing following weight/mass percentage composition in red ganoderma extract reference substance：Ganoderic acid A be 0.3%-0.5%, glossy ganoderma
Sour B is 0.15%-0.4%, and ganoderic acid C 2 is 0.3%-0.5%, and Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- is 0.25%-0.5%.
Preferably, for prepare red ganoderma extract reference substance red ganoderma raw medicinal material be at least two red ganoderma medicines
Material.
Further, also include to the ganoderic acid A in red ganoderma extract reference substance, ganoderic acid B, ganoderic acid C 2, glossy ganoderma
The step of content of sour G is demarcated.
Preferably, the preparation of the red ganoderma extract comprises the steps：Red ganoderma is crushed, solvent extraction is added,
Extract is filtered, filtrate is collected；Preferably, described extract using heating and refluxing extraction, ultrasonic extraction, Microwave Extraction,
Seepage pressure effects or homogenate extraction.
Preferably, also include carrying out filtrate reduced pressure concentration, add in concentrate auxiliary material, continue to be evaporated to dry
Step；Preferably, the auxiliary material is dextrin, superfine silica gel powder, starch, at least one in carboxymethylcellulose calcium；It is furthermore preferred that institute
The mass ratio for stating auxiliary material and the concentrate is 0.5: 1～3: 1.
Preferably, the solvent is methyl alcohol, ethanol, the methanol aqueous solution that volumetric concentration is more than 70% or volumetric concentration
At least one in more than 70% ethanol water.The ethanol water of methyl alcohol or volumetric concentration 95% is wherein most preferably.
Preferably, also include to being used for preparing the step screened by the red ganoderma raw medicinal material of red ganoderma extract reference substance
Suddenly, the screening step comprises the steps：
Red ganoderma standard diagram is set up, glossy ganoderma medicinal material to be selected is carried out liquid-phase chromatographic analysis and by the collection of illustrative plates for being obtained and institute
State red ganoderma standard diagram to be compareed, filter out medicinal material of the similarity more than 90%；
It is further preferred that the medicinal material by the similarity more than 90% carries out strain idenfication, red ganoderma is confirmed as in screening
Medicinal material；Still more preferably, the medicinal material to filtering out carries out content analysis, filters out ganoderic acid A mass contents and is more than
0.02%th, ganoderic acid B mass content is more than 0.015%, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- mass content more than 0.01%, 2 mass content of ganoderic acid C
Medicinal material more than 0.015%.
Used as a kind of specific embodiment, the red ganoderma standard diagram is set up in accordance with the following steps：
Glossy ganoderma medicinal material from different sources is carried out thin layer collection of illustrative plates discriminating, and the thin layer collection of illustrative plates with red ganoderma control medicinal material
Contrasted, selected the medicinal material with the thin layer collection of illustrative plates of red ganoderma control medicinal material fluorescence spot of aobvious same color on relevant position
For preferred medicinal material；
Preferred medicinal material is obtained finger-print by liquid-phase chromatographic analysis, and gained finger-print input Chinese medicine chromatogram is referred to
Line collection of illustrative plates similarity evaluation system carries out fingerprint map analyzing and generates red ganoderma standard diagram.
Used as a kind of preferred version, the chromatographic condition of the liquid chromatogram includes：Chromatographic column selects reverse-phase chromatographic column, flowing
Mutually include mobile phase A and Mobile phase B, mobile phase A is trifluoroacetic acid, formic acid, acetic acid, at least one in phosphoric acid and methyl alcohol, second
At least one mixed solution in nitrile, Mobile phase B are trifluoroacetic acid, formic acid, acetic acid, at least one water-soluble in phosphoric acid
Liquid；Preferably, the mobile phase A be 0.05～0.15% (quality) trifluoroacetic acid acetonitrile solution, Mobile phase B be 0.05～
0.15% (quality) trifluoroacetic acid aqueous solution；It is further preferred that the gradient elution program of mobile phase is：A+B=100% (bodies
Product), in 0-35min, mobile phase A maintains 20-30% (volume), and Mobile phase B maintains 70-80% (volume)；In 35-
90min, mobile phase A maintain 60-70% (volume), and Mobile phase B maintains 30-40% (volume)；In 90-120min, flowing
Phase A maintains 100% (volume), and Mobile phase B maintains 0% (volume).
Used as a kind of preferred version, the thin layer collection of illustrative plates differentiates to carry out according to following parameter：
Lamellae is silica gel thin-layer plate, and solvent is the toluene of volume ratio 12～17: 12～17: 0.5～1.5: 0.1, second
Acetoacetic ester, methyl alcohol, formic acid, developer are mass fraction 10%-50% ethanol solution of sulfuric acid.
Second aspect present invention provides a kind of red ganoderma extract reference substance, including each group of following weight/mass percentage composition
Point：Ganoderic acid A is 0.3%-0.5%, and ganoderic acid B is 0.15%-0.4%, and ganoderic acid C 2 is 0.3%-0.5%, and Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- is
0.25%-0.5%.；And/or, the red ganoderma extract reference substance is obtained using preparation method as described above.
Red ganoderma extract reference substance mentioned above can be applicable to the quality evaluation of red ganoderma medicinal material and contain red ganoderma
Application in terms of the quality evaluation of component product
The technical scheme that the present invention is provided has the advantages that：
The present invention is prepared for a kind of red ganoderma extract reference substance as the tester of red ganoderma quality evaluation.The present invention is carried
For red ganoderma extract reference substance in the following areas have notable superiority：
1st, compared with ganodenic acid chemical reference substance
1) with the use of single compound (or several compounds combine) is reference substance ratio, carried using the red ganoderma of the present invention
Take thing reference substance to differentiate medicinal material, the profile information for more enriching can be assumed on thin-layer chromatography and liquid chromatogram, be easy to not
With the discriminating between lucidum variety.
2) existing ganodenic acid chemical reference substance extraction and purification process is complicated, with high costs, it is difficult in production practices
Promote.And the red ganoderma extract reference substance preparation process is simple of the present invention, reproducible, disclosure satisfy that qualitative and quantitative analysis
The requirement of ganoderic acid in glossy ganoderma.
3) can be realized with a red ganoderma extract reference substance while to red spirit using the red ganoderma extract reference substance
Multiple ganoderic acid compounds contents in sesame sample are demarcated.And use ganodenic acid chemical reference substance then can only demarcate every time
A ganoderic acid composition in red ganoderma sample.Therefore, the time of detection is greatlyd save using red ganoderma extract reference substance
And cost.
2nd, compared with control medicinal material：
1) the extract reference substance using the present invention differentiates that medicinal material has more preferable stability and higher versatility.Control
Medicinal material can be affected to produce larger batch wise differences by natural environment, and extract reference substance is due to realizing production technology
Regulation and control and raw material blend, and demarcate through further strict, can preferably ensure the homogeneity between batch.
2) in addition, extract reference substance need not be processed before complexity when using, and directly can be used after dissolving.Than
Control medicinal material convenience ease in use.
3) control medicinal material is only used for the Qualitive test of red ganoderma medicinal material or red ganoderma product, it is impossible to for red ganoderma medicinal material
The quantitative determination of middle ganodenic acid.And red ganoderma extract reference substance both can be used for determining for red ganoderma medicinal material or red ganoderma product
Property differentiate, it is also possible to for the quantitative determination of ganodenic acid in red ganoderma medicinal material.
In sum, red ganoderma extract reference substance can realize glossy ganoderma control medicinal material and glossy ganoderma chemical reference substance simultaneously
Function, and a red ganoderma extract reference substance can be realized to demarcating while multiple glossy ganoderma compositions, easy to use, cost
Low.It is a kind of reference substance for more conforming to international standard.
Description of the drawings
Fig. 1 is the thin-layer chromatogram of sample 1- samples 18；
Fig. 2 is the thin-layer chromatogram of sample 19- samples 30；
Fig. 3 is the thin-layer chromatogram of sample 31- samples 39；
Fig. 4 is the thin-layer chromatogram of sample 40- samples 45；
Fig. 5 is the thin-layer chromatogram of sample 46- samples 50；
Fig. 6 is the liquid-phase chromatograph finger print atlas analysis chart of 30-50 glossy ganoderma medicinal materials；
Fig. 7 is red ganoderma standard diagram；
Fig. 8 is to differentiate different cultivars glossy ganoderma with red ganoderma extract reference substance thin layer；
Fig. 9 is red ganoderma medicinal material assay figure.
Specific embodiment
The present invention provides a kind of red ganoderma extract reference substance, the preparation of the red ganoderma extract reference substance mainly include as
Lower step：The step of red ganoderma being extracted to obtain red ganoderma extract, different red ganoderma extracts is mutually blent
With obtain red ganoderma extract reference substance the step of, one of purpose that blends be in order to allow red ganoderma extract reference substance reach as
Lower quality standard, i.e. each component containing following weight/mass percentage composition：Ganoderic acid A is 0.3%-0.5%, and ganoderic acid B is
0.15%-0.4%, ganoderic acid C 2 are 0.3%-0.5%, and Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- is 0.25%-0.5%.The quality standard is that the application sends out
After person of good sense's red ganoderma medicinal material multiple batches of to different sources carries out quality research and with reference to extraction process and detection method, comprehensively comment
The result that valency draws.When red ganoderma extract reference substance meets the standard, the items in red ganoderma extract reference substance are main
Composition can embody the feature of red ganoderma medicinal material active ingredient, and when checking for liquid chromatogram and thin-layer chromatography, its each group
The separating degree of part disclosure satisfy that the requirement that qualitative, quantitative is determined.
Described blending is to blend the red ganoderma extract of different batches extraction or two or more red ganoderma medicinal materials.Red
Ganodenna Lucidum P.E can be a kind of red ganoderma medicinal material or two or more red ganoderma medicinal material extract gained；More preferably scheme is,
Prepared using two or more red ganoderma medicinal materials, such that it is able to make the obtained band for extracting reference substance more rich, heterogeneity
Content also more uniform, so as to obtain the extract reference substance of more high-quality.
The present invention provides a kind of red ganoderma extract reference substance, and its preparation is still further comprised to ganoderic acid A therein, spirit
The step of sesame acid B, the content of ganoderic acid C 2, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- are demarcated.
The preparation of red ganoderma extract is preferably carried out according to the method for comprising the steps：Red ganoderma is crushed, is added molten
Agent is extracted, and extract is filtered, and collects filtrate.Preferably, described extract using heating and refluxing extraction, ultrasonic extraction, micro-
Ripple extraction, seepage pressure effects or homogenate extraction, are more highly preferred to, and described extraction adopts heating and refluxing extraction or ultrasonic extraction.It is preferred that
, also include the step of reduced pressure concentration is carried out to filtrate；It is further preferred that adding auxiliary material in also including to concentrate and continuing
It is evaporated to dry step.It is further preferred that auxiliary material be dextrin, superfine silica gel powder, starch, in carboxymethylcellulose calcium at least
One kind, is more highly preferred to, and auxiliary material adopts superfine silica gel powder, as superfine silica gel powder is water insoluble and organic solvent, will not be to reference substance
Interference is brought for subsequent detection；Preferably, the mass ratio of auxiliary material and concentrate is 0.5: 1～3: 1, and concrete addition is according to dense
The quality of contracting thing is adjusted, and makes the quality of final extract reference substance reach standard.Extract solvent used preferred
For at least one in methyl alcohol, ethanol, the concentration range of solvent is preferably 70～100%, more preferably 85-95%, most preferably
For 95% ethanol and pure methyl alcohol.
As a kind of preferred version, for the red ganoderma raw medicinal material for being used for preparing red ganoderma extract reference substance is sieved
Choosing, it is preferred to use pass through the red ganoderma of screening as raw medicinal material.
Can be used to prepare red ganoderma extract reference substance through medicinal material of the strain idenfication for red ganoderma；But, excellent as one kind
Scheme is selected, the red ganoderma medicinal material for being used for preparing red ganoderma extract reference substance is preferably sieved using the method for comprising the steps
Choosing：
Red ganoderma standard diagram is set up, red ganoderma medicinal material to be selected is carried out liquid-phase chromatographic analysis and by the collection of illustrative plates for being obtained
Compareed with the red ganoderma standard diagram, filtered out medicinal material of the similarity more than 90%.
Further, screening step can also include, carry out strain idenfication to medicinal material of the similarity more than 90%, filter out
It is defined as the medicinal material of red ganoderma.Further, the medicinal material of preferred pair screening gained carries out content analysis, filters out ganoderic acid A
Mass content is more than 0.02%, ganoderic acid B mass content more than 0.01%, 2 mass content of ganoderic acid C more than 0.015%, glossy ganoderma
Medicinal material of the sour G mass contents more than 0.015%.The quality standard is present inventor's red ganoderma multiple batches of to different sources
After medicinal material carries out quality research, the result that overall merit draws.When red ganoderma medicinal material meets the standard, with the red ganoderma medicinal material
Prepared extract reference substance is more easy to the quality standard for meeting red ganoderma extract reference substance.
As a kind of specific embodiment, for the foundation of above red ganoderma standard diagram can be entered in accordance with the following steps
OK：The collected glossy ganoderma medicinal material from different sources is carried out thin layer collection of illustrative plates discriminating, and the thin layer with red ganoderma control medicinal material
Collection of illustrative plates is contrasted, and selects the fluorescence spot for showing same color with the thin layer collection of illustrative plates of red ganoderma control medicinal material on relevant position
Medicinal material is preferred medicinal material；Preferred medicinal material is obtained finger-print by liquid-phase chromatographic analysis, and during gained finger-print is input into
Medicine chromatographic fingerprinting similarity evaluation system (2012.1 editions) carries out fingerprint map analyzing and generates red ganoderma standard diagram.For
Make red ganoderma standard diagram representative, city should be able to be represented for generating the red ganoderma medicinal material sample of red ganoderma standard diagram
The quality of red ganoderma class product on field, in specific embodiment below, specifically have chosen 20 from 50 glossy ganoderma samples
Red ganoderma sample is used for generating red ganoderma standard diagram, but does not indicate that the standard diagram that more or less of sample is generated just does not have
Representative.
As preferred, in order to allow ganoderic acid A, B, C2, G all preferably to be separated, carry out being adopted during liquid-phase chromatographic analysis
Chromatographic condition is preferably included：
The preferred reverse-phase chromatographic column of chromatographic column, more preferably C18 ODS reverse-phase chromatographic columns.
Preferably, mobile phase includes mobile phase A and Mobile phase B, mobile phase A be trifluoroacetic acid, formic acid, acetic acid, in phosphoric acid
At least one at least one mixed solution with methyl alcohol, in acetonitrile, Mobile phase B is trifluoroacetic acid, formic acid, acetic acid, phosphoric acid
In at least one aqueous solution；It is furthermore preferred that the mobile phase A is that 0.05～0.15% (quality) trifluoroacetic acid acetonitrile is molten
Liquid, Mobile phase B are 0.05～0.15% (quality) trifluoroacetic acid aqueous solution；
It is further preferred that the gradient elution program of mobile phase is：Following percentage is percent by volume, A+B=
100%, in 0-35min, mobile phase A maintains 20-30%, and Mobile phase B maintains 70-80%；In 35-90min, mobile phase A
60-70% is maintained, Mobile phase B maintains 30-40%；In 90-120min, mobile phase A maintains 100%, and Mobile phase B is maintained
0%.
It is further preferred that thin layer collection of illustrative plates differentiates to carry out according to following parameter：Lamellae adopts silica gel thin-layer plate, solvent
For the toluene of volume ratio 12～17: 12～17: 0.5～1.5: 0.1, ethyl acetate, methyl alcohol, formic acid, developer is mass fraction
10%-50% ethanol solution of sulfuric acid.Using preferred thin layer collection of illustrative plates discrimination condition, differentiate compared to the thin layer collection of illustrative plates of Chinese Pharmacopoeia
, can there is more multi-ribbon in method, it can be clearly seen that the difference between different glossy ganodermas, so as to preferably differentiate glossy ganoderma medicinal material.
Technical scheme is described further with specific embodiment below in conjunction with the accompanying drawings：
The foundation of 1 red ganoderma standard diagram of embodiment
1.1st, General description of experiments：
Thin layer collection of illustrative plates discriminating is carried out to the 50 kinds of glossy ganoderma medicinal materials (numbering is No. 1-50 respectively) from different sources that collects,
Filter out on its thin-layer chromatography position corresponding with red ganoderma control medicinal material chromatogram, show the glossy ganoderma medicine of the fluorescence spot of same color
Material, 20 kinds altogether.Fingerprint map analyzing is carried out with liquid chromatography to 20 kinds of red ganodermas, gained collection of illustrative plates is input into Chinese medicine chromatogram
Fingerprint similarity evaluation system (2012.1 editions) generates red ganoderma standard diagram (see Fig. 7)
1.2nd, 50 kinds of glossy ganoderma medicinal material indentification by TLC
1.2.1, the sample preparation of 50 kinds of glossy ganoderma medicinal materials
2g glossy ganoderma medicinal materials are weighed, 30ml methyl alcohol ultrasound (150w, 44khz) 30min are added, is filtered, filter residue adds 30ml first
Alcohol ultrasound 30min, filters, and filter paper is washed with a small amount of methyl alcohol, and 2ml volumetric flasks are dissolved and be settled to water bath method filtrate with methyl alcohol
In.Obtain final product.
Red ganoderma control medicinal material is purchased from middle inspection institute.
1.2.2, thin layer differentiates chromatographic condition：
1.2.3, experimental result：
Indentification by TLC is carried out to 50 glossy ganoderma medicinal materials, Fig. 1 to Fig. 5 is shown in by thin layer collection of illustrative plates.1- can be drawn by thin layer collection of illustrative plates
The thin layer collection of illustrative plates of No. 30 samples is inconsistent with red ganoderma control medicinal material thin layer collection of illustrative plates, the thin layer collection of illustrative plates of 31-50 samples and red spirit
Sesame control medicinal material thin layer collection of illustrative plates is consistent.Therefore, 31-50 samples are chosen as the sample for generating red ganoderma standard diagram.
In Fig. 1-Fig. 5, the sample representated by label is respectively：1. the black sesame in Hebei, 2. Jilin purple sesame 1,3. Jilin purple sesame 2,
4. Jilin purple sesame 3,5. Dalian glossy ganoderma 1,6. Dalian glossy ganoderma 2, the 7. black sesame 1 of Nankunshan, the 8. black sesame 2 of Nankunshan, the 9. black sesame of Nankunshan
3rd, 10. Yunnan Wild purple sesame 1,11. Yunnan Wild purple sesames 2, the black sesame in 12. Fujian, the black sesame in 13. Sichuan, the black sesame 1,15. in 14. Jiangxi
The black sesame 2 in Jiangxi, the wild purple sesame in 16. Hubei, the wild purple sesame in 17. Anhui, 18. Hainan ganoderma lipsienses, 19. Yunnan honeycomb glossy ganodermas 20.
Yunnan red fungus sesame, 21. U.S. sheet, the black sesame 1 in 22. Yunnan, the black sesame in 23. Tibet, the black sesame 1 in 24. Hubei, the black sesame in 25. Hubei
2nd, the black sesame 3 in 26. Hubei, the black sesame 2 in 27. Yunnan, the black sesame 1 of 28. Yunnan Wilds, the black sesame 2 of 29. Yunnan Wilds, 30. Shandong river sesames, 31.
Jilin red ganoderma 1,32. Jilin red ganodermas 2,33. Zhejiang red ganodermas 1,34. Zhejiang red ganodermas 2,35. Shanxi red ganodermas 1,36. mountains
Western red ganoderma 2,37. Fujian red ganodermas, 38. Yunnan red ganodermas 1,39. Yunnan red ganodermas 2,40. Hainan red ganodermas, 41. Jilin are red
Glossy ganoderma, 42. Shandong red ganodermas, 43. Lishui of Zhejiang red ganodermas, 44. Anhui red ganodermas 1,45. Anhui red ganodermas 2,46. Jilin dragon
Well red ganoderma 1,47. Longjing County of Jilin, Northeast China red ganodermas 2,48. Shandong red ganodermas 1,49. Shandong red ganodermas 2,50. Shandong red ganodermas 3.
1.3rd, red ganoderma standard diagram is generated
1.3.1, the sample preparation of 20 kinds of red ganoderma medicinal materials
2g red ganoderma medicinal materials are weighed, 30ml methyl alcohol ultrasound (150w, 44khz) 30min are added, is filtered, filter residue adds 30ml
Methyl alcohol ultrasound 30min, filters, and filter paper is washed with a small amount of methyl alcohol, and 2ml capacity is dissolved and be settled to water bath method filtrate with methyl alcohol
In bottle.0.22 μm of miillpore filter crossed by sample, obtains final product.
1.3.2, liquid phase chromatogram condition
Chromatographic column：TSK gel ODS-80Tm(5μm 150*4.6mm)
Mobile phase：According to the form below ratio, gradient elution
| Step | Time (min) | 0.1% trifluoroacetic acid acetonitrile (%) | 0.1% trifluoroacetic acid water (%) |
| 1 | 0 | 26 | 74 |
| 2 | 35 | 26 | 74 |
| 3 | 90 | 65 | 35 |
| 4 | 120 | 100 | 0 |
Flow velocity：1.2mL/min；Column temperature：25℃；Detection wavelength：256nm sample sizes：10μl
1.3.3, experimental result：
Liquid-phase chromatograph finger print atlas input chromatographic fingerprints of Chinese materia medica similarity evaluation system by 30-50 red ganoderma samples
System (2012.1 editions), analysis result is as shown in fig. 6, the red ganoderma standard diagram for generating is as shown in Figure 7.
Embodiment 2 is used for the preferred of red ganoderma raw material prepared by red ganoderma extract reference substance
2.1st, General description of experiments
The red ganoderma raw material prepared for red ganoderma extract reference substance need to meet following quality standard：
(1) its collection of illustrative plates of medicinal material liquid chromatography analysis is contrasted with red ganoderma standard diagram, and similarity should be greater than 90%
(using similarity evaluation 2012.1 editions)
(2) ganoderic acid A contents be more than 0.02%, ganoderic acid B content be more than 0.01%, 2 content of ganoderic acid C be more than
0.015%, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- content is more than 0.015%.
According to above-mentioned quality standard, from preferred feedstock medicinal material in 6 kinds of red ganoderma samples, method is as follows：
2.2nd, raw medicinal material chromatograms and red ganoderma standard diagram similarity-rough set
2.2.1, the preparation of 6 kinds of red ganoderma samples
2g red ganoderma medicinal materials are weighed, 30ml methyl alcohol ultrasound (150w, 44khz) 30min are added, is filtered, filter residue adds 30ml
Methyl alcohol ultrasound 30min, filters, and filter paper is washed with a small amount of methyl alcohol, and 2ml capacity is dissolved and be settled to water bath method filtrate with methyl alcohol
In bottle.0.22 μm of miillpore filter crossed by sample, obtains final product.
2.2.2, liquid phase chromatogram condition
Chromatographic column：TSK gel ODS-80Tm(5μm 150*4.6mm)
Mobile phase：According to the form below ratio, gradient elution
Flow velocity：1.2mL/min；Column temperature：25℃；Detection wavelength：256nm sample sizes：10μl
2.2.3, experimental result：
The liquid chromatogram collection of illustrative plates of 6 kinds of red ganoderma medicinal materials is contrasted with red ganoderma standard diagram, similarity result such as table 1 below institute
Show.Therefore 1-2 Sample Similarities cannot function as the raw material of red ganoderma extract reference substance less than 90%.3-6 samples are similar
Degree carries out strain idenfication through delivering to Institute of Microorganism, Academia Sinica, is defined as red ganoderma more than 90%.
Table 1
| Sample | Similarity |
| Red ganoderma 1 | 86.9% |
| Red ganoderma 2 | 88.5% |
| Red ganoderma 3 | 92.5% |
| Red ganoderma 4 | 95.2% |
| Red ganoderma 5 | 91.2% |
| Red ganoderma 6 | 94.3% |
2.3rd, raw medicinal material ganodenic acid assay
2.3.1, chemical reagent：
Ganoderic acid A, ganoderic acid B, ganoderic acid C 2, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- are purchased from Shanghai Yuan Ye Bioisystech Co., Ltd
2.3.2, experimental technique：
The preparation of 4 kinds of red ganoderma samples and liquid-phase chromatography method are ibid.
Preparing for ganoderic acid reference substance laboratory sample is as follows：Weigh ganoderic acid A, B, C2, G reference substance 5mg respectively, use methyl alcohol
Dissolving is settled in 5ml volumetric flasks.0.22 μm of miillpore filter is crossed, is obtained final product.
2.3.3, experimental result：
The ganodenic acid assay result of 3-6 samples is as shown in table 2 below, draws from data in table 2：No. 3 sample spirits
Sesame acid B content is less than standard, and No. 42 contents of sample ganoderic acid C are less than standard.5-6 sample ganoderic acid contents meet standard, because
This, chooses No. 5 and No. 6 samples as the raw medicinal material for preparing red ganoderma extract reference substance.
Table 2
Prepared by 3 red ganoderma extract reference substance of embodiment
3.1st, General description of experiments
Red ganoderma extract prepares solvent for use for methyl alcohol, and extracting mode is to be heated to reflux.
3.2nd, experimental technique
Preferably go out in embodiment 22 kinds of red ganoderma medicinal materials are prepared into red ganoderma extract respectively, preparation method is as follows：
Weigh red ganoderma medicinal material 2kg crushing, add the methyl alcohol 16L of 8 times of amounts, heating and refluxing extraction twice, 1 hour (liquid boiling every time
Start timing), filtration is collected filtrate, is evaporated to 5L at 65 DEG C, each addition auxiliary material superfine silica gel powder 120g, continues concentration
To dry, each 200g of red ganoderma extract is obtained, numbering is No. 1 red ganoderma extract, No. 2 red ganoderma extracts respectively.
No. 1 red ganoderma extract 200g, No. 2 red ganoderma extract 200g are weighed, is well mixed, add methyl alcohol 4L, stirring
Mix fully dissolving, be concentrated to dryness, obtain blending rear red ganoderma extract.
Prepared by 4. red ganoderma extract reference substance of embodiment
4.1 General description of experiments
Red ganoderma extract prepares solvent for use for methyl alcohol, and extracting mode is ultrasonic extraction
4.2 experimental technique
Preferably go out in embodiment 22 kinds of red ganoderma medicinal materials are prepared into red ganoderma extract respectively, preparation method is as follows：
Red ganoderma medicinal material 2kg crushing is weighed, the methyl alcohol 16L of 8 times of amounts is added, twice, 1 hour every time, filter was collected in filtration to ultrasonic extraction
Liquid, is evaporated to 5L at 65 DEG C, each addition auxiliary material superfine silica gel powder 120g, continues to be concentrated to dryness, obtains red ganoderma extract
Each 200g, numbering is No. 1 red ganoderma extract, No. 2 red ganoderma extracts respectively.
No. 1 red ganoderma extract 200g, No. 2 red ganoderma extract 200g are weighed, is well mixed, add methyl alcohol 4L, stirring
Mix fully dissolving, be concentrated to dryness, obtain blending rear red ganoderma extract.
Prepared by 5. red ganoderma extract reference substance of embodiment
5.1 General description of experiments
Red ganoderma extract prepares solvent for use for 95% ethanol, and extracting mode is to be heated to reflux
5.2 experimental technique
Preferably go out in embodiment 22 kinds of red ganoderma medicinal materials are prepared into red ganoderma extract respectively, preparation method is as follows：
Weigh red ganoderma medicinal material 2kg crushing, add 95% ethanol 16L of 8 times of amounts, heating and refluxing extraction twice, 1 hour (liquid every time
Boiling starts timing), filtration is collected filtrate, is evaporated to 5L at 65 DEG C, respectively adds auxiliary material micro mist 120g silica gel, continues
It is concentrated to dryness, obtains each 200g of red ganoderma extract, numbering is No. 1 red ganoderma extract, No. 2 red ganoderma extracts respectively.
No. 1 red ganoderma extract 200g, No. 2 red ganoderma extract 200g are weighed, are well mixed, add 95% ethanol 4L,
Stirring mixes fully dissolving, is concentrated to dryness, obtains blending rear red ganoderma extract.
Prepared by 6. red ganoderma extract reference substance of embodiment
6.1 General description of experiments
Red ganoderma extract prepares solvent for use for 95% ethanol, and extracting mode is ultrasonic extraction
6.2 experimental technique
Preferably go out in embodiment 22 kinds of red ganoderma medicinal materials are prepared into red ganoderma extract respectively, preparation method is as follows：
Red ganoderma medicinal material 2kg crushing is weighed, 95% ethanol 16L of 8 times of amounts is added, ultrasonic extraction twice, 1 hour every time, collected by filtration
Filtrate, is evaporated to 5L at 65 DEG C, each addition auxiliary material micro mist 120g silica gel, continues to be concentrated to dryness, obtains red ganoderma extraction
The each 200g of thing, numbering is No. 1 red ganoderma extract, No. 2 red ganoderma extracts respectively.
No. 1 red ganoderma extract 200g, No. 2 red ganoderma extract 200g are weighed, are well mixed, add 95% ethanol 4L,
Stirring mixes fully dissolving, is concentrated to dryness, obtains blending rear red ganoderma extract.
Embodiment 7 demarcates red ganoderma extract reference substance
7.1. General description of experiments
With ganoderic acid A, ganoderic acid B, ganoderic acid C 2, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- as reference substance, red ganoderma is demarcated with liquid chromatography and extracted
Ganoderic acid A, ganoderic acid B, the content of ganoderic acid C 2, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- in thing reference substance.
7.2. reagent
Ganoderic acid A, ganoderic acid B, ganoderic acid C 2, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- are purchased from Shanghai Yuan Ye Bioisystech Co., Ltd；
Red ganoderma extract reference substance prepared by embodiment 3.
7.2.1. the sample preparation of red ganoderma extract reference substance and ganoderic acid reference substance
0.2g red ganoderma extract reference substances are weighed, is dissolved with methyl alcohol and is settled in 2ml volumetric flasks.Sample crosses 0.22 μm
Miillpore filter, obtains final product.
Weigh ganoderic acid A, B, C2, G reference substance 5mg respectively, be settled in 5ml volumetric flasks with methyl alcohol dissolving.Cross 0.22 μm
Miillpore filter, obtains final product.
7.2.2. liquid phase chromatogram condition
Chromatographic column：TSK gel ODS-80Tm(5μm 150*4.6mm)
Mobile phase：According to the form below ratio, gradient elution
Flow velocity：1.2mL/min；Column temperature：25℃；Detection wavelength：256nm sample sizes：10μl
7.2.3. experimental result：
The ganoderic acid content that is demarcated in red ganoderma extract reference substance with ganoderic acid chemical reference substance, content data such as following table
Shown in 3.In the method, the separating degree of ganoderic acid A, B, C2, G is all higher than 0.9, and theoretical cam curve is all higher than 6000, RSD and is respectively less than
1%, therefore the method can be used for demarcate red ganoderma extract reference substance.Red ganoderma is demarcated with ganoderic acid chemical reference substance to extract
Thing reference substance content, ganoderic acid A, B, C2, G content of red ganoderma extract reference substance is：Ganoderic acid A：0.4108%, ganoderic acid
B：0.2286%, ganoderic acid C 2：0.3402%, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)-：0.3168%.The red ganoderma extract reference substance meets red ganoderma
(ganoderic acid A is 0.3%-0.5% to the quality standard of extract reference substance, and ganoderic acid B is 0.15%-0.4%, and ganoderic acid C 2 is
0.3%-0.5%, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- are 0.25%-0.5%).
Table 3
8 red ganoderma extract reference substance stability of embodiment
8.1. General description of experiments
Accelerated stability investigation is carried out to red ganoderma extract reference substance with accelerated stability experiment.
8.2. accelerated stability experimental technique
The red ganoderma extract reference substance for preparing is placed in：Temperature：40 DEG C, humidity：Under the conditions of 90% ± 5%, place
0th, 1,2,3,6 months, red ganoderma extract reference substance was taken out at 0,1,2,3,6 months, carried with liquid chromatography for measuring red ganoderma
Take ganoderic acid content in thing reference substance.
8.3. reagent
Ganoderic acid A, ganoderic acid B, ganoderic acid C 2, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- are purchased from Shanghai Yuan Ye Bioisystech Co., Ltd, accelerate steady
Qualitative experiment 0-6 month sample
8.3.1. the sample preparation of red ganoderma extract reference substance
0.2g red ganoderma extract reference substances are weighed, is dissolved with methyl alcohol and is settled in 2ml volumetric flasks.Sample crosses 0.22 μm
Miillpore filter, obtains final product.
Weigh ganoderic acid A, B, C2, G reference substance 5mg respectively, be settled in 5ml volumetric flasks with methyl alcohol dissolving.Cross 0.22 μm
Miillpore filter, obtains final product.
8.3.2. liquid phase chromatogram condition
Chromatographic column：TSK gel ODS-80Tm(5μm 150*4.6mm)
Mobile phase：According to the form below ratio, gradient elution
| Step | Time (min) | 0.1% trifluoroacetic acid acetonitrile (%) | 0.1% trifluoroacetic acid water (%) |
| 1 | 0 | 26 | 74 |
| 2 | 35 | 26 | 74 |
| 3 | 90 | 65 | 35 |
| 4 | 120 | 100 | 0 |
Flow velocity：1.2mL/min；Column temperature：25℃；Detection wavelength：256nm sample sizes：10μl
8.3.3. experimental result
Accelerated stability experimental data such as table 4 below, ganoderic acid in accelerated stability 0-6 month red ganoderma extract reference substance
The RSD values of A, B, c2, G are less than 1%, reproducible.Therefore the shelf-life of the red ganoderma extract reference substance is tentative 24 months.Table
4 stability datas for being specially reference substance obtained in embodiment 3, the experimental result of embodiment 4-6 and 3 basic simlarity of embodiment,
Repeat no more.
Table 4：Red ganoderma extract reference substance accelerated stability data
9 red ganoderma extract thin layer of embodiment differentiates different cultivars glossy ganoderma
9.1. General description of experiments
Differentiate lucidum variety with red ganoderma extract reference substance and thin layer chromatography, discrimination method is as follows：
9.2. the preparation of laboratory sample
0.2g red ganoderma extract reference substances (3 product of embodiment) are weighed, and 2ml volumetric flasks are dissolved and are settled to methyl alcohol
In.0.22 μm of miillpore filter crossed by sample, obtains final product.
2g red ganodermas medicinal material and lucidum spore powder is weighed, 30ml methyl alcohol ultrasound (150w, 44khz) 30min are added, is filtered,
Filter residue adds 30ml methyl alcohol ultrasound 30min, filters, and filter paper is washed with a small amount of methyl alcohol, and water bath method filtrate is dissolved with methyl alcohol and determined
In holding to 2ml volumetric flasks.Obtain final product.
9.2.1. thin-layer chromatography condition：
9.2.2. experimental result
Fig. 8 is shown in by the collection of illustrative plates of thin-layer chromatography detection, as can be seen from the figure No. 2 and No. 3 its thin-layer chromatography of sample and comparison medicine
Wood color composes the fluorescence spot for showing same color on corresponding position, and collection of illustrative plates spectral pattern is consistent, and therefore confirmatory sample No. 2 and No. 3 is red
Glossy ganoderma.As can be seen from the figure, on No. 4 to No. 7 sample its thin-layer chromatographys position corresponding with control medicinal material chromatogram, show phase
With the fluorescence spot of color, collection of illustrative plates spectral pattern is inconsistent, and therefore confirmatory sample No. 4 to No. 7 is not red ganoderma medicinal material.Therefore, use
Red ganoderma extract reference substance is that tester can distinguish different cultivars glossy ganoderma in thin-layer chromatography detection.
In Fig. 8, each label is represented respectively：1. red ganoderma extract reference substance, 2. red ganoderma 1,3. red ganoderma 2,4. shoulder four spirit
Sesame, 5. ganoderma lipsiense, 6. purple sesame, 7. lucidum spore powder.
Embodiment 10 demarcates same red ganoderma medicinal material glossy ganoderma with red ganoderma extract reference substance and ganoderic acid chemical reference substance
Acid content
10.1. General description of experiments
Same red spirit is demarcated with red ganoderma extract reference substance and ganoderic acid chemical reference substance and with liquid-phase chromatography method
Sesame medicinal material, its method are as follows：
10.2. reagent
Red ganoderma extract reference substance (3 product of embodiment), red ganoderma medicinal material, ganoderic acid A, ganoderic acid B, ganoderic acid C 2,
Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- is purchased from Shanghai Yuan Ye Bioisystech Co., Ltd
10.2.1. sample preparation
2g red ganoderma medicinal materials are weighed, 30ml methyl alcohol ultrasound (150w, 44khz) 30min are added, is filtered, filter residue adds 30ml
Methyl alcohol ultrasound 30min, filters, and filter paper is washed with a small amount of methyl alcohol, and 2ml capacity is dissolved and be settled to water bath method filtrate with methyl alcohol
In bottle.0.22 μm of miillpore filter crossed by sample, obtains final product.
Weigh 0.2g red ganoderma extract reference substance methyl alcohol to dissolve and be settled in 2ml volumetric flasks.Sample crosses 0.22 μm
Miillpore filter, obtains final product.
Weigh ganoderic acid A, B, C2, G reference substance 5mg respectively, be settled in 5ml volumetric flasks with methyl alcohol dissolving.Cross 0.22 μm
Miillpore filter, obtains final product.
10.2.2. liquid phase chromatogram condition
Chromatographic column：TSK gel ODS-80Tm(5μm 150*4.6mm)
Mobile phase：According to the form below ratio, gradient elution
| Step | Time (min) | 0.1% trifluoroacetic acid acetonitrile (%) | 0.1% trifluoroacetic acid water (%) |
| 1 | 0 | 26 | 74 |
| 2 | 35 | 26 | 74 |
| 3 | 90 | 65 | 35 |
| 4 | 120 | 100 | 0 |
Flow velocity：1.2mL/min；Column temperature：25℃；Detection wavelength：256nm sample sizes：10μl
10.2.3. experimental result
With red ganoderma extract reference substance demarcate red ganoderma medicinal material in ganoderic acid content with use ganoderic acid chemical reference substance
The data for demarcating the content of ganoderic acid in red ganoderma medicinal material are shown in Table 5.As can be seen from Table 5：Demarcated with two kinds of scaling methods
Ganoderic acid content in red ganoderma medicinal material its content difference is respectively less than 2%, RSD values and is respectively less than 1%, and separating degree is all higher than 0.9, manages
6000 are all higher than by the number of plates.Therefore red ganoderma extract reference substance can be used to substitute ganoderic acid chemical reference substance as tester
Ganoderic acid content in detection red ganoderma.
Table 5：Two kinds of different demarcation modes demarcate the data of ganoderic acid content
Embodiment 11 determines red ganoderma medicinal material ganoderic acid content with red ganoderma extract reference substance
11.1. General description of experiments
With ganoderic acid content in red ganoderma extract reference substance and liquid chromatography for measuring red ganoderma medicinal material, method is as follows：
11.2. sample preparation
2g medicinal materials are weighed, 30ml methyl alcohol ultrasound (150w, 44khz) 30min are added, is filtered, filter residue adds 30ml methyl alcohol to surpass
Sound 30min, filters, and filter paper is washed with a small amount of methyl alcohol, and water bath method filtrate is dissolved with methyl alcohol and is settled in 2ml volumetric flasks.Sample
Product cross 0.22 μm of miillpore filter, obtain final product.
0.2g red ganoderma extract reference substances (3 product of embodiment) are weighed, and 2ml volumetric flasks are dissolved and are settled to methyl alcohol
In.0.22 μm of miillpore filter crossed by sample, obtains final product.
11.2.1. liquid phase chromatogram condition
Chromatographic column：TSK gel ODS-80Tm(5μm 150*4.6mm)
Mobile phase：According to the form below ratio, gradient elution
Flow velocity：1.2mL/min；Column temperature：25℃；Detection wavelength：256nm sample sizes：10μl
11.2.2. experimental result
The data that ganoderic acid content in red ganoderma medicinal material is determined with red ganoderma extract reference substance are shown in Table in 6 and Fig. 9, Fig. 9
Abscissa is numbered for medicinal material, and ordinate is ganoderic acid content.As can be seen from Table 6：Red ganoderma medicinal material in 13 samples for determining
Middle numbering is that the sample ganoderic acid content of No. 3 and No. 11 is far above other samples, content more than 4mg/g, numbering is 1,2,4,5-8
Number sample ganoderic acid content relatively low, less than 1mg/g, the ganoderic acid content of other samples is between 1mg/g-3mg/g.
It is therefore evident that：The quality that red ganoderma quality of medicinal material can be identified with red ganoderma extract.
Table 6：Ganoderic acid content in red ganoderma medicinal material is determined with red ganoderma extract reference substance
The above, is only presently preferred embodiments of the present invention, not does any pro forma restriction to the present invention, therefore
All contents without departing from technical solution of the present invention, any simply repair to made for any of the above embodiments according to the technical spirit of the present invention
Change, equivalent variations and modification, still fall within the range of technical solution of the present invention.
Claims (13)
1. a kind of preparation method of red ganoderma extract reference substance, it is characterised in that include red ganoderma is extracted to obtain
The step of red ganoderma extract, also include mutually blending to obtain red ganoderma extract reference substance by different red ganoderma extracts
The step of, each component containing following weight/mass percentage composition in the red ganoderma extract reference substance：Ganoderic acid A is 0.3%-
0.5%, ganoderic acid B is 0.15%-0.4%, and ganoderic acid C 2 is 0.3%-0.5%, and Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- is 0.25%-0.5%.
2. preparation method according to claim 1, it is characterised in that for preparing the red spirit of red ganoderma extract reference substance
Sesame raw medicinal material is at least two red ganoderma medicinal materials.
3. preparation method according to claim 1, it is characterised in that also include to the spirit in red ganoderma extract reference substance
The step of sesame acid A, ganoderic acid B, the content of ganoderic acid C 2, Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- are demarcated.
4. preparation method according to claim 1, it is characterised in that the preparation of the red ganoderma extract includes following step
Suddenly：Red ganoderma is crushed, solvent extraction is added, extract is filtered, collect filtrate；Preferably, the extraction is adopted and is added
Circumfluence distillation, ultrasonic extraction, Microwave Extraction, seepage pressure effects or homogenate extraction.
5. preparation method according to claim 4, it is characterised in that also include carrying out filtrate reduced pressure concentration, to concentration
Auxiliary material is added in thing, continue to be evaporated to dry step；Preferably, the auxiliary material is dextrin, superfine silica gel powder, starch, carboxylic first
At least one in base cellulose；Preferably, the mass ratio of the auxiliary material and the concentrate is 0.5: 1～3: 1.
6. preparation method according to claim 4, it is characterised in that the solvent is methyl alcohol, ethanol or volumetric concentration
At least one in the ethanol water of more than 70% methanol aqueous solution or volumetric concentration more than 70%, wherein preferred methyl alcohol
Or the ethanol water of volumetric concentration 95%.
7. preparation method according to claim 1, it is characterised in that also include to for preparing the control of red ganoderma extract
The step of red ganoderma raw medicinal material of product is screened, the screening step comprise the steps：
Red ganoderma standard diagram is set up, glossy ganoderma medicinal material to be selected is carried out the liquid-phase chromatographic analysis collection of illustrative plates for being obtained is red with described
Glossy ganoderma standard diagram is compareed, and filters out medicinal material of the similarity more than 90%.
8. preparation method according to claim 7, it is characterised in that also comprise the steps, the similarity is more than
90% medicinal material carries out strain idenfication, and the medicinal material of red ganoderma is confirmed as in screening；Preferably, the medicinal material to filtering out carries out content point
Analysis, filters out ganoderic acid A mass contents more than 0.02%, ganoderic acid B mass content more than 0.01%, 2 mass content of ganoderic acid C
More than medicinal material of 0.015%, the Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- mass content more than 0.015%.
9. preparation method according to claim 7, it is characterised in that the red ganoderma standard diagram is built in accordance with the following steps
Vertical：
Glossy ganoderma medicinal material from different sources is carried out thin layer collection of illustrative plates discriminating, and is carried out with the thin layer collection of illustrative plates of red ganoderma control medicinal material
Contrast, the medicinal material for selecting the fluorescence spot for showing same color on relevant position with the thin layer collection of illustrative plates of red ganoderma control medicinal material are excellent
Select medicinal material；
Preferred medicinal material is obtained finger-print by liquid-phase chromatographic analysis, and gained finger-print is input into Chinese medicine chromatographic fingerprint figure
Spectrum similarity evaluation system carries out fingerprint map analyzing and generates red ganoderma standard diagram.
10. the preparation method according to claim 7 or 9, it is characterised in that the chromatographic condition of the liquid chromatogram includes：
Chromatographic column selects reverse-phase chromatographic column, mobile phase to include mobile phase A and Mobile phase B, mobile phase A be trifluoroacetic acid, formic acid, acetic acid,
At least one mixed solution at least one and methyl alcohol, acetonitrile in phosphoric acid, Mobile phase B are trifluoroacetic acid, formic acid, second
At least one aqueous solution in acid, phosphoric acid；Preferably, the mobile phase A is 0.05～0.15% trifluoroacetic acid acetonitrile solution,
Mobile phase B is 0.05～0.15% trifluoroacetic acid aqueous solution；It is further preferred that the gradient elution program of mobile phase is：A+B=
100%, in 0-35min, mobile phase A maintains 20-30%, and Mobile phase B maintains 70-80%；In 35-90min, mobile phase A
60-70% is maintained, Mobile phase B maintains 30-40%；In 90-120min, mobile phase A maintains 100%, and Mobile phase B is maintained
0%.
11. preparation methods according to claim 9, it is characterised in that the thin layer collection of illustrative plates differentiates to enter according to following parameter
OK：
Lamellae is silica gel thin-layer plate, and solvent is the toluene of volume ratio 12～17: 12～17: 0.5～1.5: 0.1, acetic acid second
Ester, methyl alcohol, formic acid, ethanol solution of sulfuric acid of the developer for mass fraction 10%-50%.
12. a kind of red ganoderma extract reference substances, it is characterised in that including each component of following weight/mass percentage composition：Ganoderic acid A
For 0.3%-0.5%, ganoderic acid B is 0.15%-0.4%, and ganoderic acid C 2 is 0.3%-0.5%, and Lanost-8-en-26-oic acid,3,7,12-trihydroxy-11,15,23-trioxo-,(3BETA,7BETA,12BETA)- is 0.25%-
0.5%.；And/or, the red ganoderma extract reference substance is using the preparation method system as described in any one of claim 1～11
?.
Red ganoderma extract reference substance described in 13. any one of claim 1～12 red ganoderma medicinal material quality evaluation and contain
The application having in terms of the quality evaluation of red ganoderma component product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610821305.8A CN106501439B (en) | 2016-09-13 | 2016-09-13 | A kind of red ganoderma extract reference substance and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610821305.8A CN106501439B (en) | 2016-09-13 | 2016-09-13 | A kind of red ganoderma extract reference substance and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106501439A true CN106501439A (en) | 2017-03-15 |
| CN106501439B CN106501439B (en) | 2019-03-12 |
Family
ID=58290057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610821305.8A Active CN106501439B (en) | 2016-09-13 | 2016-09-13 | A kind of red ganoderma extract reference substance and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106501439B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106946966A (en) * | 2017-04-29 | 2017-07-14 | 北京林业大学 | A kind of subcritical water law extracts wild red ganoderma ganoderic acid A and C simultaneously2Method |
| CN107807182A (en) * | 2017-09-28 | 2018-03-16 | 株洲千金药业股份有限公司 | The content assaying method of ganoderic acid A compositions in Ganoderma Syrupus |
| CN108003211A (en) * | 2017-12-22 | 2018-05-08 | 福建农大菌草技术开发公司 | A kind of method of quick separating ganoderic acid in accessory substance from ganoderma lucidum |
| CN109932441A (en) * | 2019-03-01 | 2019-06-25 | 贵州瑞和制药有限公司 | A kind of method for building up of easypro liver injection for curing HPLC finger-print |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5057224A (en) * | 1990-04-11 | 1991-10-15 | National Science Council | Method for evaluating the performance of reverse phase high performance liquid chromatographic columns |
| CN101530436A (en) * | 2009-04-20 | 2009-09-16 | 浙江省林业科学研究院 | Method of effectively extracting ganoderma triterpenoids and ganoderan from mythic fungus germ entity |
| US20110059124A1 (en) * | 2008-02-26 | 2011-03-10 | Guangzhou Hanfang Natural Medicine Research & Development Co.Ltd | The quality control method and application of a kind of ganoderma lucidum spore oil fat emulsion |
| CN102735772A (en) * | 2012-07-02 | 2012-10-17 | 福建省药品检验所 | Quality controlling method of ganoderma aqueous extract |
| CN103710421A (en) * | 2014-01-07 | 2014-04-09 | 天津市林业果树研究所 | Method for establishing lucid ganoderma sporophore HPLC (High Performance Liquid Chromatography) finger-print spectrum |
| CN104237398A (en) * | 2013-06-13 | 2014-12-24 | 陈浩达 | Calibration method of reference extracts and application of calibration method |
| CN105385745A (en) * | 2015-12-18 | 2016-03-09 | 安发（福建）生物科技有限公司 | Ganoderic acid extraction and analysis method |
-
2016
- 2016-09-13 CN CN201610821305.8A patent/CN106501439B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5057224A (en) * | 1990-04-11 | 1991-10-15 | National Science Council | Method for evaluating the performance of reverse phase high performance liquid chromatographic columns |
| US20110059124A1 (en) * | 2008-02-26 | 2011-03-10 | Guangzhou Hanfang Natural Medicine Research & Development Co.Ltd | The quality control method and application of a kind of ganoderma lucidum spore oil fat emulsion |
| CN101530436A (en) * | 2009-04-20 | 2009-09-16 | 浙江省林业科学研究院 | Method of effectively extracting ganoderma triterpenoids and ganoderan from mythic fungus germ entity |
| CN102735772A (en) * | 2012-07-02 | 2012-10-17 | 福建省药品检验所 | Quality controlling method of ganoderma aqueous extract |
| CN104237398A (en) * | 2013-06-13 | 2014-12-24 | 陈浩达 | Calibration method of reference extracts and application of calibration method |
| CN103710421A (en) * | 2014-01-07 | 2014-04-09 | 天津市林业果树研究所 | Method for establishing lucid ganoderma sporophore HPLC (High Performance Liquid Chromatography) finger-print spectrum |
| CN105385745A (en) * | 2015-12-18 | 2016-03-09 | 安发（福建）生物科技有限公司 | Ganoderic acid extraction and analysis method |
Non-Patent Citations (6)
| Title |
|---|
| USP: "《Herbal Medicines Compendium》", 16 July 2014 * |
| YU-ZHEN YAN ET AL: "Study on Triterpenoic Acids Distribution in Ganoderma Mushrooms by Automatic Multiple Development High Performance Thin Layer Chromatographic Fingerprint Analysis", 《JOURNAL OF AOAC INTERNATIONAL》 * |
| 国家食品药品监督管理局药品审评中心 编: "《药品技术评价文集》", 31 December 2006 * |
| 李国华 等: "灵芝三萜类化合物研究进展", 《中草药》 * |
| 郭晓蕾 等: "不同产地灵芝( 赤芝) 的HPLC 指纹图谱评价", 《中华中医药学刊》 * |
| 陈沛 等: "对照提取物在中药整体质量控制中的应用", 《药物分析杂志》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106946966A (en) * | 2017-04-29 | 2017-07-14 | 北京林业大学 | A kind of subcritical water law extracts wild red ganoderma ganoderic acid A and C simultaneously2Method |
| CN107807182A (en) * | 2017-09-28 | 2018-03-16 | 株洲千金药业股份有限公司 | The content assaying method of ganoderic acid A compositions in Ganoderma Syrupus |
| CN107807182B (en) * | 2017-09-28 | 2020-09-25 | 株洲千金药业股份有限公司 | Method for measuring content of ganoderic acid A in ganoderma lucidum syrup |
| CN108003211A (en) * | 2017-12-22 | 2018-05-08 | 福建农大菌草技术开发公司 | A kind of method of quick separating ganoderic acid in accessory substance from ganoderma lucidum |
| CN109932441A (en) * | 2019-03-01 | 2019-06-25 | 贵州瑞和制药有限公司 | A kind of method for building up of easypro liver injection for curing HPLC finger-print |
| CN109932441B (en) * | 2019-03-01 | 2022-08-19 | 贵州瑞和制药有限公司 | Method for establishing HPLC fingerprint of Shuganning injection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106501439B (en) | 2019-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106501439A (en) | A kind of red ganoderma extract reference substance and its preparation method and application | |
| CN106338563B (en) | A kind of quality evaluating method of red ganoderma medicinal material | |
| CN104922196B (en) | The preparation of small pagodatree flower general flavone extract and quality determining method | |
| CN113138239B (en) | Small radix bupleuri compound preparation fingerprint spectrum and construction method thereof and content determination method of small radix bupleuri compound preparation | |
| CN106198837A (en) | The quality determining method of old cough with asthma sheet | |
| CN105588885A (en) | Salvianolic acid extract fingerprint spectrum and content measurement method of related components | |
| CN113063885B (en) | Composition for preparing Baoyuan decoction, baoyuan decoction product and fingerprint spectrum measuring and quality detecting method thereof | |
| CN109444290A (en) | The construction method and detection method of Asiatic plantain medicinal material UPLC characteristic spectrum | |
| CN104569192A (en) | Method for detecting quality of moghania macrophylla | |
| CN104997840A (en) | Dracocephalum heterophyllum Benth pentacyclic triterpene component sample pretreatment method and use of Dracocephalum heterophyllum Benth pentacyclic triterpene component | |
| CN106483210B (en) | A kind of detection method of red ganoderma medicinal material | |
| CN101791366A (en) | Method for testing quality of Discorea nipponica Makino in different places and medicinal materials of same genera | |
| CN113267578B (en) | Quality control method of peony and licorice decoction | |
| CN101703610A (en) | Quality detection method of Qingnao antihypertensive tablet | |
| CN105628851A (en) | Detection method of traditional Chinese medicine preparations | |
| CN107884508A (en) | The quality determining method of Yinhuang lung clearing capsule | |
| CN110487951A (en) | A kind of quality determining method of Hedan tablet | |
| CN109696513A (en) | A method of identifying ginseng type and its growth year using ginsenoside component content and its ratio | |
| CN109655572A (en) | A kind of thin layer chromatography of quick identification Yupingfeng Granules ingredient | |
| CN115078608A (en) | Method for identifying UPLC characteristic spectrums of abrus cantoniensis hance and abrus pubescens hance | |
| CN101987115A (en) | Jade screen oral preparation as well as preparation method and detection method thereof | |
| CN107607665A (en) | One seedling medicine subprostrate sophora and its Chinese medicine preparation method of quality control | |
| CN115808473A (en) | Novel method for quality control of Chinese herbal compound angelica Sini decoction | |
| CN114324726B (en) | Thin-layer chromatography detection method of kidney-nourishing and fetus-nourishing pill | |
| CN110687219A (en) | Detection method of suhuang cough-relieving capsule fingerprint and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |