CN106501418A - A kind of flue gas causes perfumery to build method and the application of Nicotiana tabacum L. finger printing - Google Patents
A kind of flue gas causes perfumery to build method and the application of Nicotiana tabacum L. finger printing Download PDFInfo
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- CN106501418A CN106501418A CN201611269651.6A CN201611269651A CN106501418A CN 106501418 A CN106501418 A CN 106501418A CN 201611269651 A CN201611269651 A CN 201611269651A CN 106501418 A CN106501418 A CN 106501418A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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Abstract
The present invention proposes a kind of method that flue gas causes perfumery to build Nicotiana tabacum L. finger printing, including step：By different cultivars Nicotiana tabacum L. 55 DEG C in the baking oven at be respectively dried after, grind to form the following offal of 40 mesh, store for future use under drying condition；By the offal of every kind of Nicotiana tabacum L., burnt under the same conditions, burn the flue gas for producing, and filters through Nicotiana tabacum L. pyrolysis installation, and by the sucking filtration sample constant volume for obtaining, sealed, preserved；In sample, each component content is determined using online GC/MS analyses.The speciality of flue gas is produced by combining fingerprint pattern technology and Nicotiana tabacum L. burning, creative defines to causing perfumery to build Nicotiana tabacum L. fingerprint map construction in flue gas, the flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing to cause the application in perfumery analysis, the also further investigation for the local flavor of flue gas to provide new method in flue gas.
Description
Technical field
The present invention relates to detection field, particularly relates to a kind of method that flue gas causes perfumery to build Nicotiana tabacum L. finger printing, this
A kind of bright application for further relating to method of flue gas cause perfumery structure Nicotiana tabacum L. finger printing in flue gas causes perfumery to analyze.
Background technology
Nicotiana tabacum L. is with a long history in China's plantation, is one of important industrial crops of China.Quality of tobacco is to reflect and embody
The comprehensive concept of Nicotiana tabacum L. necessity character equilibrium situation, it includes presentation quality and inherent quality.For a long time, quality of tobacco master
To be evaluated by four aspects and be classified, be i.e. presentation quality, sensory evaluating smoking, chemical analyses, physical characteristics, from certain angle
See, still can not comprehensively meet requirement.As chemical analyses only have 8,9 indexs, general character is emphasized；Ocular estimate and sensory evaluating smoking receive
The impact of subjective factorss is larger.For this purpose, expert has done substantial amounts of research work both at home and abroad, to objectively and accurately evaluating Nicotiana tabacum L. matter
Amount, chemical composition analysis are exactly the main research contents of one of which.
Finger printing refers to some complex materials, such as Chinese medicine, the DNA of certain organism or certain tissue or cell, egg
After white matter is appropriately processed, using certain analysis means, the chromatogram or spectrogram that can indicate its chemical feature for obtaining.
Finger printing is broadly divided into Chinese medicine fingerprint, DNA fingerprinting and peptide fingerprinting spectrum.Chinese medicine fingerprint is one
Synthesis is planted, quantifiable identification of means, on the basis of it is built upon chemical composition of Chinese materia medica systematic study, is mainly used in evaluating
The verity of Chinese crude drug and Chinese medicine preparation semi-manufactured goods quality, Optimality and stability.It is multiple that Chinese medicine and its preparation are multicomponent
Miscellaneous system, therefore evaluates the detection method that its quality using be adapted therewith, should can provide abundant authentication information, sets up Chinese medicine
Finger printing more can will comprehensively reflect the species and quantity of contained chemical composition in Chinese medicine and its preparation, and then to medicine matter
Amount carries out whole description and evaluation.On this basis, if further carrying out spectrum effect learns research, Chinese medicine quality and its drug effect can be made
Really combine, contribute to illustrating Mechanism of TCM.In a word, the research and foundation of Chinese medicine fingerprint, for raising in
Medicine quality, promotes the modernization of Chinese medicine significant.
Product main when flue gas is tobacco sucking, the abnormal smells from the patient of each material in flue gas, for each flue-cured tobacco product of formation
Respective special flavor is planted, with important effect, wherein, the cause perfumery effect in flue gas becomes apparent from.
But at present, the detection and evaluation of these materials, is also concentrated mainly on artificial evaluation, and some simple chemistry point
Analysis, also far from enough in terms of precision, also, the differentiation between different Nicotiana tabacum Lves, trickle area
Point, the change of environmental factorss such as in some planting process aforementioned, for the adjustment of flue gas local flavor, conventional means are more difficult to area
Point, for the quality for further lifting and improving Nicotiana tabacum L., this realizes that China's " level on Medicated cigarette " strategic task causes difficulty.
Accordingly, it would be desirable to some emerging technology are fully combined, and the characteristic of flue gas this product, the detection side to flue gas
Method and mode, are all improved.
Content of the invention
The present invention proposes a kind of method that flue gas causes perfumery to build Nicotiana tabacum L. finger printing, and the further investigation for Nicotiana tabacum L. is provided
The more reliable method of science.
The technical scheme is that and be achieved in that：A kind of flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing, bag
Include step：By different cultivars Nicotiana tabacum L. 55 DEG C in the baking oven at be respectively dried after, grind to form the following offal of 40 mesh, store up under drying condition
Deposit standby；By the offal of every kind of Nicotiana tabacum L., burnt under the same conditions, burn the flue gas for producing, through Nicotiana tabacum L. pyrolysis installation mistake
Filter, and by the sucking filtration sample constant volume for obtaining, seal, preserve；In sample, each component content is determined using online GC/MS analyses.
Further, GC conditions are：Ultra-2 capillary columns, carrier gas are N2, stigma pressure 170kPa.
More accurately, every kind of Nicotiana tabacum L. weighs 8.0g powder offals.
Further, multiple extraction of reducing pressure is carried out in pressure 0.03MPa.
Further, tobacco pipe that the Nicotiana tabacum L. pyrolysis installation for wherein adopting includes being connected with each other, some bottle,suctions, regulator and
Vacuum pump, loads petroleum ether in the bottle,suction, carries out multiple extraction of reducing pressure；The petroleum ether phase flue gas merged in each bottle,suction is carried
Take sample.The quantity of wherein described bottle,suction is 4, and the bottle,suction has smoke inlet and outlet.
The flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing to cause the application in perfumery analysis in flue gas.By building
Go out to cause the finger printing of perfumery in different flue gases, form the fingerprint databases that perfumery is caused in flue gas, and cause in the flue gas
The fingerprint databases of perfumery are divided, and form classification and division to causing perfumery in different Nicotiana tabacum L. flue gases.
The flue gas of the present invention causes the method that perfumery builds Nicotiana tabacum L. finger printing, by combining fingerprint pattern technology and cigarette
Leaf burning produces the speciality of flue gas, and creative defines to cause perfumery structure Nicotiana tabacum L. fingerprint map construction in flue gas, and specifically
Employ the mode of sucking filtration extraction, it is ensured that effective acquisition of material in Nicotiana tabacum L., and prevent the damage or change of material.Described
Flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing to cause the application in perfumery analysis in flue gas, and also the local flavor for flue gas gos deep into
Research provides new method.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also
To obtain other accompanying drawings according to these accompanying drawings.
Fig. 1 is that Nicotiana tabacum L. of the present invention is pyrolyzed apparatus structure schematic diagram；
Fig. 2 is Nanxiong Guangdong cigarette 97C3F flue-cured tobaccos (2009) smoke components finger printing；
Fig. 3 is Nanxiong Guangdong cigarette 97C3F flue-cured tobaccos (2009) smoke components finger printing.
Specific embodiment
Accompanying drawing in below in conjunction with the embodiment of the present invention, to the embodiment of the present invention in technical scheme carry out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiment.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Disclosure of the invention step is as follows：
By every kind of Nicotiana tabacum L. 55 DEG C in the baking oven at be respectively dried after, grind to form the following offal of 40 mesh, store under drying condition
Standby.Every kind of Nicotiana tabacum L. weighs 8.0g powder, loads in the clean glass tobacco pipe in top, under the same conditions, with Nicotiana tabacum L. pyrolysis dress
Put and burnt, in each bottle,suction, load a small amount of petroleum ether, multiple extraction of reducing pressure is carried out in pressure 0.03MPa；Merge and respectively take out
Petroleum ether phase flue gas in filter flask extracts sample, and constant volume in 25ml volumetric flasks, sealing are placed at -4 DEG C and preserve；Each in sample
Constituent content is determined using online GC/MS analyses.Nicotiana tabacum L. pyrolysis installation is shown in Fig. 1.
GC conditions：Ultra-2 capillary columns (50m × 0.2mm. × 0.33 μm .), carrier gas is N2, stigma pressure 170kPa,
Detector FID, 280 DEG C of injector temperature, 280 DEG C of detector temperature, temperature programming:80 DEG C (1min), 2 DEG C/min, 280 DEG C
(30min), 2 μ l of sample size, split ratio 10: 1.When GC/MS is determined GC conditions ibid, ionization voltage 70ev, ion source temperature 200
DEG C, 220 DEG C of transmission line temperature carries out collection of illustrative plates retrieval using Wiley spectrums storehouse.
Embodiment 1
Nanhsiung, Kwangtung Guangdong cigarette 97C3F flue-cured tobaccos (2008) smoke components fingerprint map analyzing, as a result as follows：
(1) ketone：Hydroxypropanone- 0.61%, 2- cyclopentenones 0.92%, 3- methyl -2- hydroxyl -2- cyclopentene-1-ones
0.74%th, 2- cyclopentenes -1,4- diketone 3.14%, methyl cyclopentenyl ketone 1.91%, ethylcyclopentenolone 0.61%, 9- hydroxyls
Base -1- methyl -- 1.2.3.4- tetrahydrochysene -8H- pyridines (1.2-a) pyrazine -8- ketone 0.23%, 1- (the fluoro- beta naphthal 1- yls of 8-) ethylene
Ketone 0.61%.Ketone accounts for 8.77% that flue gas causes perfumery.
(2) aldehydes：2 furan carboxyaldehyde 23.26%, 5- methylfurans aldehyde 3.07%.Aldehydes accounts for 26.33% that flue gas causes perfumery
(3) alcohols：3,7,11- trimethyl -1- pentadecanols 1.17%, maltol 0.69%, furancarbinol 3.16%.Alcohols
Account for the 5.02% of flue gas cause perfumery.
(4) organic acid：Acetic acid 5.32%, Palmic acid 3.78%, Linolenic Acid, 12,15- trienic acids 0.73%.Organic acid
Class accounts for 9.83% that flue gas causes perfumery.
(5) esters：Dibutyl phthalate 0.40%.Esters account for 0.40% that flue gas causes perfumery.
(6) polycyclic aromatic hydro carbons：
It is not detected by the constituents.
(7) phenols：2- methoxyphenols 1.27%, phenol 6.22%, 4- methylphenols 3.69%, 3- methylphenols
0.86%th, 4- ethyl -phenols 1.49%, 2,6- syringol 0.74%.Phenols accounts for 14.27% that flue gas causes perfumery.
(8) alkanes：7- methyl-Fourth Ring (4.1.0.0 (2.4.) 0.3.5) heptane 2.28%, Pentadecane 0.31%, 1-
Acetoxyl group -2 pentanone epoxide ethane 0.24%, alkanes accounts for 2.83% that flue gas causes perfumery.
(9) unsaturated fatty acidss hydro carbons：DL- limonenes 1.27%, benzocyclobutene 0.55%, (suitable) 2,5- diformazan -3- hexenes
4.28%th, Squalene 0.39%, 2.6.10.14.18- pentamethyl -2.6.10.14.18- eicosapentaenoics 0.61%.Unsaturated
Fat hydrocarbon accounts for 7.10% that flue gas causes perfumery.
(10) alkaloidss：Nicotine 14.77%.Alkaloidss account for 14.77% that flue gas causes perfumery
(11) terpenoid：Neophytadiene 5.58%.Terpenoid is accounted for and causes the 5.58% of perfumery.
(12) other classes：1,2- methylbenzene 1.91%, 3- picolines 0.58%, 2- acetyl furans 0.58%, pyrroles
0.81%th, N- methyl succinamide 0.82%, indole 0.4%.
Table 1：The main component of Nanxiong cigarette district Nicotiana tabacum L. flavor matter
Embodiment 2
Nanxiong Guangdong cigarette 97C3F flue-cured tobaccos (2009) smoke components fingerprint map analyzing, as a result as follows：
(1) ketone：2- cyclopentenones 0.73%, methyl cyclopentenyl ketone 0.96%, 2,3- dimethyl-2-cyclopenten-1-ones
0.07%th, 3- methyl -2- cyclopentene-1-ones 0.93%, 2,3- dimethyl-2-cyclopenten-1-ones 0.96%, 3- hexene -2,5-
Diketone 1.26%, methyl cyclopentenyl ketone 3.11%, 3- ethyl -2- hydroxyl -2- cyclopentene-1-ones 1.15%, 3- ethyl -2- hydroxyls
Base -2- cyclopentene-1-ones 1.41%, 3- (2,2- dimethylpropylidenes) bicyclo- (3,3,1) nonane 1.34%, 1- indones
0.92%th, 2,5- dimethyl -4- hydroxyfuranones 1.37%.Ketone accounts for 14.21% that flue gas causes perfumery.
(2) aldehydes：
It is not detected by the constituents.
(3) alcohols：Furfuryl alcohol 2.62%, trans -16 carbon -1- alcohol 1.25%, furfuryl alcohol 1.80% of (E) -9-, 3,7,11- front threes
Base-DODECANOL, 1- 3.23%, benzyl alcohol 0.37%, alcohols account for 9.27% that flue gas causes perfumery.
(4) organic acid：Propanoic acid 0.64%, n-butyric acie 0.45%, positive valeric acid 0.98%, Palmic acid 2.17%, elaidic acid
1.87%.Organic acid accounts for 6.11% that flue gas causes perfumery
(5) esters：Diisobutyl phthalate 1.17%, dibutyl phthalate 1.28%.Esters account for flue gas and cause perfume
The 2.45%. of thing
(6) polycyclic aromatic hydro carbons：
It is not detected by the constituents.
(7) phenols：2- methoxyphenols 1.71%, 2- methoxyl group -4- methylphenols 0.41%, phenol 8.14%, 2,4- bis-
Cresols 0.68%, paracresol 6.33%, m-ethylphenol 2.17%, 2- methoxyl-4-vinyl phenols 1.70%.Phenols is accounted for
Flue gas causes the 21.14% of perfumery.
(8) alkanes：Pentadecane 0.76%, n-heptadecane 0.58%, n-octadecane 0.41%.Alkanes account for flue gas cause
The 1.75% of perfumery.
(9) unsaturated fatty acidss hydro carbons：Dipentene 1.97%, styrene 1.62%, 1-tetradecylene 0.16%, 3,6- oxo -1-
Three ring of methyl -8- isopropyls [6.2.2.0 (2,7)] 12 4,9- diene 2.71%, 2,6,10- trimethyl -1,5,9- hendecanes
Triolefin 1.20%, ten pentaenes 0.34% of 1-, 17 alkene 0.24% of 1-.Unsaturated fatty acidss hydro carbons accounts for 8.24% that flue gas causes perfumery.
(10) alkaloidss：Nicotine 15.81%.Alkaloidss account for 15.81% that flue gas causes perfumery
(11) terpenoid：Neophytadiene 15.52%.Terpenoid is accounted for and causes the 15.52% of perfumery.
(12) other classes：Ethylo benzene 0.49%, o-Dimethylbenzene 1.16%, N- (4- methoxybenzyls) acetamide 2.03%, 2- second
Acyl furan 0.4%, indole 1.44%.
Table 2：Nanxiong Guangdong cigarette 97C3F flue-cured tobaccos (2009) fume component analysis result
From above-described embodiment as can be seen that identical flue-cured tobacco cultivars same area Nicotiana tabacum L. is in different year, the Nicotiana tabacum L. of production fires
The flue gas of burning has visibly different finger printing information, strong so as to have for the accurate flue gas for dividing different flue-cured tobacco cultivars
Instrument, meanwhile, also to furtheing investigate flue gas mass, lift flue gas texture and local flavor, there is provided method.
Presently preferred embodiments of the present invention is the foregoing is only, not in order to limit the present invention, all in essence of the invention
Within god and principle, any modification, equivalent substitution and improvements that is made etc. should be included within the scope of the present invention.
Claims (8)
1. a kind of flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing, it is characterised in that including step：By different cultivars Nicotiana tabacum L.
After being respectively dried at 55 DEG C in the baking oven, the following offal of 40 mesh is ground to form, is stored for future use under drying condition；Cigarette by every kind of Nicotiana tabacum L.
End, is burnt under the same conditions, and burn the flue gas for producing, and filters through Nicotiana tabacum L. pyrolysis installation, and by the sucking filtration sample for obtaining
Constant volume, sealing are preserved；In sample, each component content is determined using online GC/MS analyses.
2. flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing as described in the appended claim 1, it is characterised in that：GC conditions are：
Ultra-2 capillary columns, carrier gas are N2, stigma pressure 170kPa.
3. flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing as described in the appended claim 1, it is characterised in that：Every kind of Nicotiana tabacum L. claims
Take 8.0g powder offals.
4. flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing as described in the appended claim 1, it is characterised in that：In pressure
Multiple extraction of reducing pressure is carried out during 0.03MPa.
5. flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing as described in the appended claim 1, it is characterised in that：Nicotiana tabacum L. pyrolysis dress
Tobacco pipe, some bottle,suctions, regulator and the vacuum pump including being connected with each other is put, is loaded petroleum ether in the bottle,suction, is carried out
Reduce pressure multiple extraction；The petroleum ether phase flue gas merged in each bottle,suction extracts sample.
6. flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing as described in the appended claim 1, it is characterised in that：The bottle,suction
Quantity be 4, the bottle,suction has smoke inlet and outlet.
7. flue gas as described in arbitrary in claim 1-6 causes the method that perfumery builds Nicotiana tabacum L. finger printing to cause perfumery analysis in flue gas
In application.
8. flue gas causes the method that perfumery builds Nicotiana tabacum L. finger printing to cause answering in perfumery analysis in flue gas as recited in claim 7
With, it is characterised in that：By constructing the finger printing that perfumery is caused in different flue gases, form the finger printing for causing perfumery in flue gas
Storehouse, and for causing the fingerprint databases of perfumery to be divided in the flue gas, formed to causing perfumery to divide in different Nicotiana tabacum L. flue gases
Class and division.
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| CN201611269651.6A CN106501418A (en) | 2016-12-30 | 2016-12-30 | A kind of flue gas causes perfumery to build method and the application of Nicotiana tabacum L. finger printing |
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Citations (2)
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|---|---|---|---|---|
| US20100317027A1 (en) * | 2004-10-19 | 2010-12-16 | Poole Leslie B | Sulfenic acid-reactive compounds and their methods of synthesis and use in detection or isolation of sulfenic acid-containing compounds |
| CN104931301A (en) * | 2015-06-25 | 2015-09-23 | 华南理工大学 | Incense smoke collection device and method for detecting volatile ingredients of incense smoke |
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2016
- 2016-12-30 CN CN201611269651.6A patent/CN106501418A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100317027A1 (en) * | 2004-10-19 | 2010-12-16 | Poole Leslie B | Sulfenic acid-reactive compounds and their methods of synthesis and use in detection or isolation of sulfenic acid-containing compounds |
| CN104931301A (en) * | 2015-06-25 | 2015-09-23 | 华南理工大学 | Incense smoke collection device and method for detecting volatile ingredients of incense smoke |
Non-Patent Citations (3)
| Title |
|---|
| 刘帅帅等: "南平烤烟GC-MS指纹图谱构建及模式识别", 《华北农学报》 * |
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