CN106497812A - Simple bacillus cereuss and microbial inoculum and their application and heavy metal-passivated method - Google Patents
Simple bacillus cereuss and microbial inoculum and their application and heavy metal-passivated method Download PDFInfo
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Abstract
The present invention relates to microorganism field, specifically discloses a kind of simple bacillus cereuss (Bacillus simplex) and its application, the deposit number of the simple bacillus cereuss is CGMCC No.10853.Also disclose a kind of microbial inoculum containing the simple bacillus cereuss and heavy metal-passivated method.The bacterial strain of the present invention can be in Cd2+Grow up in the culture medium of 300mg/L, less than the Cd of 100mg/L2+Which is grown and is had no significant effect, show extremely strong resistance to cadmium characteristic；The Cd of the work of the bacterial strain, dead thalline in water body to low concentration2+Clearance can respectively up to 70% and more than 90%, the high concentrations of Cd to 300mg/L2+Clearance up to 30% and more than 50%；In native phase, heavy metal cadmium effectively can be changed into other stable forms from exchangeable species by the dead of the bacterial strain, viable bacteria body, and heavy metal cadmium has good passivation effect.Also, the simple bacillus cereuss heavy metal passivation of the present invention, easy to operate, energy consumption is little, does not result in secondary pollution, and the cycle is shorter, with higher economic benefit and social benefit.
Description
Technical field
The present invention relates to one plant of simple bacillus cereus, in particular it relates to one plant of simple bacillus cereus with heavy metal-passivated function and its application, and the microbial inoculum containing the simple bacillus cereuss and a kind of heavy metal-passivated method.
Background technology
China's heavy metal pollution problem becomes increasingly conspicuous, and becomes one of hot issue of environmental science concern.Due to long-term metal smelt, mining and rural sewage disposal farm, cause in soil, to be enriched substantial amounts of heavy metal contaminants, cause healthy and safe to arable soil quality and the people's livelihood greatly threatens.The survey result of national soil pollution situation is shown according to Ministry of Environmental Protections in 2014 and Ministry of Land and Resources; some areas heavy metal pollution of soil is serious; nearly 20,000 hectares by heavy metal pollution site areas such as cadmium, arsenic, lead, chromium; account for the 1/5 of total cultivated area; wherein national soil heavy metal cadmium exceeding standard rate is polluted the most serious up to 7%.
Heavy metal cadmium develops non-essential element as body growth, is one of heavy metal element most strong to plant, animal and human toxicity.Heavy metal cadmium produces biological magnification through food chain, and enrichment in higher organism is got up, and then enters human body by channels such as foods.When heavy metal cadmium in people's cylinder accumulation to certain limit, the acute or chronic poisoning of human body can be caused, kidney is particularly to human organ and causes very big harm.
Heavy metal cadmium problems demand is solved.Conventional physical restoration mainly has gas phase extraction technique and thermal desorption technology at present；Chemical restoration mainly has elution technique, fixing/stabilising technology and oxidation-reduction technology；Biological renovation method mainly has phytoremediation technology and microorganism remediation technology.
Although, a series of problems, such as there are secondary pollution, insufficient funds in physico-chemical process instant effect；Plant method in biological renovation method is a kind of eco-friendly recovery technique, but its repairing efficiency is long, it is impossible to meet the quick demand that administers.Microorganism remediation technology is passivated heavy metal in environment using the product in microorganism itself and its metabolic process, reduce heavy metal biological and can utilize degree, little to environmental perturbation, compare other recovery techniques and there are eco-friendly absolute advantagess, it is considered to be the recovery technique of most prospect in terms of heavy metal pollution.
Screening heavy metal has the bacterial strain of stronger resistance and passivation ability, and the biology passivation of heavy metal is most important.Although having reported more cadmium resistant strain at present, it really is able to be directly used in the few of cadmium pollution soil biological restoration on a large scale.
Therefore, it is badly in need of the heavy metal that one plant of exploitation can be in strength efficient degradation environment, the especially microbial strains of cadmium.
Content of the invention
The purpose of the present invention is in order to overcome prior art repairing heavy metal pollution quantities and energy consumption big, or easily cause secondary pollution, or the defect of repairing efficiency length, there is provided one plant can be under conditions of simple operations, bacterial strain and its application that the short heavy metal of free of contamination and repairing efficiency is passivated.
To achieve these goals, on the one hand, the invention provides a kind of simple bacillus cereuss (Bacillus simplex), wherein, the deposit number of the simple bacillus cereuss is CGMCC No.10853.
Second aspect, the invention provides a kind of microbial inoculum, wherein, the microbial inoculum contains the thalline of simple bacillus cereuss (Bacillus simplex) as above.
Preferably, the microbial inoculum contains the dead thalline of the simple bacillus cereuss (Bacillus simplex)
The third aspect, the invention provides the application of simple bacillus cereuss as above and/or microbial inoculum as above in heavy metal passivation.
Preferably, the heavy metal is cadmium.
Fourth aspect, the invention provides a kind of heavy metal-passivated method, the method includes：Simple bacillus cereuss (Bacillus simplex) as above are incited somebody to action, and/or microbial inoculum as above is added in the environment containing heavy metal, to be passivated to the heavy metal.
Preferably, the heavy metal is cadmium.
The bacterial strain that the present invention is provided can be in Cd2+Grow at concentrations up in the culture medium of 300mg/L, Cd of the concentration less than 100mg/L2+The growth of the bacterial strain is had no significant effect, extremely strong resistance to cadmium characteristic is shown；Cd of the viable bacteria body and dead thalline of the bacterial strain in water body to low concentration2+Clearance can respectively up to 70% and more than 90%, the high concentrations of Cd to 300mg/L2+Clearance up to 30% and more than 50%；In soil is mutually tested, heavy metal cadmium effectively can be changed into other stable forms from exchangeable species by the dead thalline of the bacterial strain, viable bacteria body, and heavy metal cadmium has good passivation effect.Also, the simple bacillus cereuss heavy metal passivation using the present invention, easy to operate, energy consumption is little, does not result in secondary pollution, and the cycle is shorter, with higher economic benefit and social benefit.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Biological deposits
The bacterial strain of the present invention is simple bacillus cereuss (Bacillus simplex), and is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address on May 22nd, 2015：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode：100101) (depositary institution is abbreviated as CGMCC), deposit number are CGMCC No.10853.
Description of the drawings
Accompanying drawing is for providing a further understanding of the present invention, and constitutes the part of description, is used for explaining the present invention, but is not construed as limiting the invention together with detailed description below.In the accompanying drawings：
Fig. 1 is the growth curve in water phase of the simple bacillus cereuss of present invention offer under different cadmium concentrations.
Specific embodiment
Hereinafter the specific embodiment of the present invention is described in detail.It should be appreciated that specific embodiment described herein is merely to illustrate and explains the present invention, the present invention is not limited to.
In a first aspect, the invention provides a kind of simple bacillus cereuss (Bacillus simplex), wherein, the deposit number of the simple bacillus cereuss is CGMCC No.10853.
The simple bacillus cereuss (Bacillus simplex) of the present invention are isolated from the cigarette of Xuancheng Profile, anhui Province and plant soil.
The detached method can be the conventional method for new strains screening in this area, for example, liquid phase concentration method or soil ring current method.
Liquid phase concentration method can specifically include：Weigh appropriate cadmium pollution soil sample to be added in the triangular flask equipped with LB fluid mediums, add appropriate bead.By triangular flask at 28-30 DEG C, vibrate under 150-170rpm；Mixing with soil liquid is proceeded to centrifuge tube, the source that the supernatant after centrifugation is passivated microorganism as cadmium is taken.The supernatant is inoculated in the LB fluid mediums containing cadmium, at 28-30 DEG C, shaken cultivation under 150-170rpm.1-2mL is drawn with aseptic straw, is moved in another enrichment culture triangular flask.After so shifting three times, in different Cd2+Plate streaking is carried out on the LB solid mediums of concentration, separates single bacterium colony.Wherein, the Cd in LB solid mediums respectively containing 50mg/L, 100mg/L, 200mg/L, 300mg/L concentration2+.
Soil ring current method can specifically include：The sand grains that cadmium pollution soil is weighed with appropriate particle diameter about 3mm is mixed homogeneously, and is placed in the upper strata of circulating device.Lower floor loads LB culture medium as circulation liquid.Start air compressor, start domestication process, period periodically adds circulation liquid according to the evaporation situation of circulation liquid.Domestication terminate after, take upper layer of soil and lower floor's circulation liquid, by domestication after microorganism in different Cd2+Plate streaking is carried out on the LB solid mediums of concentration, separates single bacterium colony.Wherein, the Cd in LB solid mediums respectively containing 50mg/L, 100mg/L, 200mg/L, 300mg/L concentration2+.
The present invention obtains one plant of most strong antibacterial of heavy metal cadmium resistance from the bacterial strain for filtering out has carried out DNA extraction and identification, and qualification result shows, 16S rDNA sequences gb of the bacterial strain | and KM817245.1 is (such as SEQ ID No：Shown in 1) there is 100% homology with simple bacillus cereuss (Bacillus simplex), can be identified as the simple bacillus cereuss of simple bacillus cereuss (Bacillus simplex), and China Committee for Culture Collection of Microorganisms's common micro-organisms center is deposited on May 22nd, 2015, deposit number is CGMCC No.10853.
The simple bacillus cereuss that the present invention is provided can produce the viable bacteria body of a large amount of simple bacillus cereuss through culture, and the method for the culture is not particularly required, as long as the simple bacillus cereuss propagation can be made, for example can be according to 107The inoculum concentration of CFU/mL is inoculated in the viable bacteria body of simple bacillus cereuss in LB culture medium, and under aerobic condition, after cultivating 8-72 hours, obtains culture fluid at a temperature of 25-38 DEG C.
The present invention can further separate the thalline of the simple bacillus cereuss in above-mentioned culture fluid, the detached method has no particular limits, as long as thalline can be enriched with from culture fluid, the method that centrifugation can be passed through for example and/or filtered is realized, the condition of the centrifugation and the filtration can be known condition, and the present invention will not be described here.
Second aspect, the invention provides a kind of microbial inoculum, wherein, the microbial inoculum contains the thalline of simple bacillus cereuss (Bacillus simplex) as above.
The present inventor is found surprisingly that the dead thalline and viable bacteria body of the simple bacillus cereuss of the present invention effectively can be passivated to metal in the course of the study.Therefore, the thalline of simple bacillus cereuss as above can be viable bacteria body, alternatively dead thalline, can also be the mixing thalline of viable bacteria body and dead thalline.But more it is surprising that it was found by the inventors of the present invention that the present invention provide simple bacillus cereuss (Bacillus simplex) dead thalline heavy metal passivation effect more preferably.
According to the present invention, for the preparation method of above dead thalline has no particular limits, such as but not limited to, can be prepared by cracking naturally, it is also possible to prepared by Thermal killed.
According to the present invention, described in the microbial inoculum, the concentration of simple bacillus cereuss has no particular limits, and specifically can be selected according to specific circumstances, and in this not go into detail.
In addition, different according to predetermined purposes, the microbial inoculum that the present invention is provided can be prepared as different dosage forms, and add the compositions such as corresponding excipient.Wherein, add which kind of excipient is known to those skilled in the art in the microbial inoculum of which kind of dosage form, in this not go into detail.
It was found by the inventors of the present invention that although the simple bacillus cereuss (Bacillus simplex) that the present invention is provided are in high Cd2+Under concentration, screening is obtained, but which to conventional polluted heavy metals in soil, for example, nickel, arsenic, copper, lead, hydrargyrum etc. have certain passivation.
Based on above discovery, the third aspect, the invention provides the application of simple bacillus cereuss as above and/or microbial inoculum as above in heavy metal passivation.
The simple bacillus cereuss heavy metal that the present invention is provided, for example, cadmium, nickel, arsenic, copper, lead, hydrargyrum etc. have certain passivation, but which is more suitable for heavy metal cadmium and is passivated.
In the application, term " passivation " is the surface for instigating heavy metal to be fixed on contaminated soil, reduces the biological effectiveness of heavy metal, reduces plant and which is absorbed, so as to reach the purpose of the environment of repairing heavy metal pollution.
Fourth aspect, the invention provides a kind of heavy metal-passivated method, the method includes：Simple bacillus cereuss (Bacillus simplex) as above are incited somebody to action, and/or microbial inoculum as above is added in the environment containing heavy metal, to be passivated to the heavy metal.
The heavy metal in the present invention specifically chosen can be as above, be not repeated herein.
According to the present invention, the environment containing heavy metal pollution can include any environment containing heavy metal, and for example, the environment containing heavy metal pollution can be included containing heavy metal, preferably cadmium, soil or water body.
According to the present invention, the form of the simple bacillus cereuss in adding to the environment containing heavy metal pollution is not particularly limited, as long as the simple bacillus cereuss can work in the environment containing heavy metal pollution and the heavy metal is effectively passivated after ensureing to add, the form of the described simple bacillus cereuss for adding, for example, can be viable bacteria body, or dead thalline, or can be the form of bacterium solution or dry powder.As above, more preferably, therefore, the present invention is preferably added to the dead thalline of the simple bacillus cereuss to the passivation effect of the dead thalline heavy metal of the simple bacillus cereuss that the present invention is provided.
The present invention is also had no particular limits to the amount of the simple bacillus cereuss for adding, this can determine according to the content of the heavy metal in the environment containing heavy metal pollution and passivation complexity, for example, when the content of beary metal in the environment is higher or during more difficult passivation, can improve the addition of the simple bacillus cereuss；When the content of beary metal in the environment is relatively low or is easier to be passivated, it is possible to reduce the addition of the simple bacillus cereuss.
In addition, when the viable bacteria body of the simple bacillus cereuss for the present invention being added in environment, can be to add the appropriate nutrient substance for being suitable to the simple bacillus cereuss growth in environment, for example, LB culture fluid, or inorganic salt culture fluid.The inorganic salt culture fluid for example can the KH containing 0.1-0.2%2PO4, the K of 0.1-0.2%2HPO4, the NH of 0.05-0.1%4NO3, the MgSO of 0.05-0.1%4, the CaCl of 0.001-0.002%2, the FeSO of 0.01-0.02%4·7H2O；PH=6.8-7.0.Wherein, each component content is in parts by weight.
According to the present invention, when the environment containing heavy metal is soil, in order to further promote the passivation efficiency of the simple bacillus cereuss heavy metal of present invention offer, preferably, water content in soil is controlled at least 15 weight %, more preferably 18-30 weight %.
Hereinafter will be described the present invention by embodiment.
In following examples：
LB fluid mediums：The peptone of 0.8-1%, the yeast powder of 0.5-0.8%, the sodium chloride of 1-1.5%, pH=6.8-7.0.Wherein, each component content is in parts by weight.
The bacterial strain of the present invention is simple bacillus cereuss (Bacillus simplex) and is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address on May 22nd, 2015：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode：100101) (depositary institution is abbreviated as CGMCC), deposit number are CGMCC No.10853.
Reference Bacillus cercuses (Bacillus cereus), Huang F, Dang Z, Guo C L, et al.Biosorption of Cd (II) by live and dead cells of Bacillus cereus RC-1isolated from cadmium-contaminated soil [J] .Colloids and Surfaces B:Biointerfaces,2013,
107:11-18.
Preparation example
The Bacillus cercuses of the simple bacillus cereuss (Bacillus simplex) and reference of the present invention are activated 2 times in LB fluid mediums, the activation carries out 10-12 hours at 28-30 DEG C, and cell concentration reaches 109CFU/mL, respectively obtains the Bacillus cercuses (bacterial strain D) of the simple bacillus cereuss (bacterial strain Cd-w) of the present invention of activation and the reference of activation, saves backup respectively at 4 DEG C.
Embodiment 1
The present embodiment is used for patience of the simple bacillus cereuss cadmium of the present invention to cadmium to be described
The bacterial strain Cd-w of activation is inoculated in LB fluid mediums, cultivate at 28-30 DEG C to 10-12h, then the fluid medium that a certain amount of bacterium solution (1-2 volume % inoculum concentrations) accesses different cadmium concentrations (respectively 0mg/L, 50mg/L, 100mg/L, 200mg/L and 300mg/L) is taken, 28-30 DEG C, 150-170rpm shaken cultivation, different time is sampled, OD value of the bacterial strain at 600nm wavelength is determined with ultraviolet spectrophotometer, to determine the growth curve of its different cadmium concentration hypothallus.Each process arranges three repetitions.Growth curve is shown in Fig. 1.
As seen from Figure 1, Cd2+Concentration be less than 100mg/L when, the bacterial strain can normal growth, growth curve is not affected by affecting, in Cd2+During at concentrations up to 300mg/L, the maximum OD600 values of the bacterial strain still can reach 4.66 ± 0.24, show the bacterial strain tolerance extremely strong to the adaptability of extreme environment and heavy metal cadmium.
Comparative example 1
The present embodiment is used for patience of the Bacillus cercuses cadmium of reference to cadmium to be described
Method according to embodiment 1 is tested to the resistance to cadmium characteristic of bacterial strain D.As a result show, bacterial strain D is merely able to the Cd in 300mg/L2+OD values under concentration only up to enough reach 2.31.And Cd2+When concentration is 100mg/L, the growth of the Bacillus cercuses has been have impact on.
Embodiment 2
The present embodiment is used for inactivating performance of the simple bacillus cereuss cadmium of present invention offer to cadmium to be described
(1) in water body
The bacterial strain Cd-w of activation is inoculated in LB liquid medium by the inoculum concentration of 1-2 volumes %, 28-30 DEG C, shaken cultivation 16-18h under 150-170rpm.Wherein,
Inactivation treatment 15-20min is carried out at one group of somatic cells is first 115-121 DEG C, then 6000-8000rpm low-temperature centrifugations 15-20min, collect dead thalline, to make dead cell passivator.
The direct 6000-8000rpm low-temperature centrifugations 15-20min of another group of somatic cells, collects viable bacteria body, and with aseptic water washing 2-3 time, to make living cells passivator.
Two groups of cells are added to containing adsorption experiment is carried out in cadmium (0mg/L, 50mg/L, 100mg/L, 200mg/L, 300mg/L) aqueous solution with the amount of 0.1g/L respectively, determine the Cd in water body after processing 3 days2+Concentration.The results are shown in Table 1.
Wherein, the detection mode of cadmium content is：The water body after appropriate process is taken, 8000rpm is centrifuged 5min, takes supernatant, with Cd in ICP-AES detection supernatant2+Concentration.
(2) in soil
Dead, living cells passivator is prepared according to method in (1), two kinds of passivator is added to sieving in air-dried soil containing heavy metal cadmium with the amount of 1g/L respectively, is stirred, at 20-24 DEG C, be passivated 10d, it is 20-30% that period keeps water content.After the completion of passivation, with the heavy metal cadmium of each form in BCR continuous extractions extraction soil, 2 are the results are shown in Table.Step is as follows：
1. exchangeable species：The air-dried pedotheque 1g by 100 mesh sieves, plus the HAc of 40mL 0.1mol/L is accurately weighed, continuous oscillation 16h at 24 ± 1 DEG C is placed in constant temperature oscillator, 15-20min then under 3000-5000rpm, is centrifuged.Supernatant is taken, and Cd is determined with ICP-AES2+Content.
2. reducible state：The residue of washing in sterilized water clear 1 is added, 20-30min is vibrated, centrifugation discards cleanout fluid.To in residue, add the oxammonium hydrochloride. of 40mL 0.5mol/L, continuous oscillation 15-16h at being placed on 22-24 DEG C in constant temperature oscillator then under 3000-5000rpm, to be centrifuged 15-20min.Remaining step same 1.
3. oxidable state：10mL H are added in 2 residue2O2, stir, the rear heating in water bath of 1h or so is stood under room temperature and keeps 80-85 DEG C of 1h or so, add 10mL H2O2, 80-85 DEG C of 1h of heating holding or so in constant water bath box.After cooling, the NH of 50mL 1mol/L is added4Ac, continuous concussion 16h at being placed on 22-24 DEG C in constant temperature vibration device, is then centrifuged 15-20min under 3000-5000rpm.Remaining step same 1.
4. remaining state：10mL HNO are added in 3 residue3, acid and sample is sufficiently mixed uniformly.Micro-wave digestion is carried out, and Cd is determined with ICP-AES2+Content.
Comparative example 2-1
This comparative example is used for inactivating performance of the strain to cadmium that reference is described
Water body and Cadmium in Soil are passivated according to the method in embodiment 2, except for the difference that, the bacterial strain for being used is bacterial strain D.1 the results are shown in Table to the inactivating performance of cadmium in water, 2 the results are shown in Table to the inactivating performance of cadmium in soil.
Comparative example 2-2
This comparative example is used for the inactivating performance that cadmium under naturalness is described
The cadmium in water body sweetening of the soil is tested according to the method in embodiment 2, except for the difference that, be added without any bacterial strain.1 the results are shown in Table to the inactivating performance of cadmium in water, 2 the results are shown in Table to the inactivating performance of cadmium in soil.
Table 1
As can be seen from Table 1, simple bacillus cereuss (Bacillus simplex) dead cell of the invention is better than living cells to the passivation effect of Heavy Metals in Waters cadmium, but the two bacterium heavy metal cadmium has larger passivation amount.And the passivation effect of simple bacillus cereuss (Bacillus simplex) heavy metal cadmium that the present invention is provided will be substantially better than the passivation effect of the Bacillus cercuses heavy metal cadmium for belonging to together of the prior art.
Table 2
As can be seen from Table 2, the cadmium of exchangeable species effectively can be transformed into more stable reducible state, oxidable state and residual form by the dead of simple bacillus cereuss of the invention, living cells passivator.Also, the effect of dead cell passivator is better than living cells passivator, this is consistent with water body passivation result.And the passivation effect of simple bacillus cereuss (Bacillus simplex) heavy metal cadmium that the present invention is provided will be substantially better than the passivation effect of the Bacillus cercuses heavy metal cadmium for belonging to together of the prior art.
Likewise, the strain of the present invention has certain passivation effect to nickel, arsenic, copper, lead, hydrargyrum in soil or water body.
The preferred embodiment of the present invention described in detail above; but, the present invention is not limited to the detail in above-mentioned embodiment, in the range of the technology design of the present invention; multiple simple variants can be carried out to technical scheme, these simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned specific embodiment, in the case of reconcilable, can be combined by any suitable means.In order to avoid unnecessary repetition, the present invention is no longer separately illustrated to various possible compound modes.
Additionally, can also carry out combination in any between a variety of embodiments of the present invention, as long as which is without prejudice to the thought of the present invention, which should equally be considered as content disclosed in this invention.
Claims (9)
1. one plant simple bacillus cereuss (Bacillus simplex), it is characterised in that the simple spore
The deposit number of bacillus is CGMCC No.10853.
2. a kind of microbial inoculum, it is characterised in that the microbial inoculum contains the simple spore bar described in claim 1
The thalline of bacterium (Bacillus simplex).
3. microbial inoculum according to claim 2, wherein, the microbial inoculum contains the simple bacillus cereuss
The dead thalline and/or viable bacteria body of (Bacillus simplex).
4. microbial inoculum according to claim 2, wherein, the microbial inoculum contains the simple bacillus cereuss
The dead thalline of (Bacillus simplex).
5. in simple bacillus cereuss described in claim 1, claim 2-4 described in any one
Application of the microbial inoculum in heavy metal passivation.
6. application according to claim 5, wherein, the heavy metal is cadmium.
7. a kind of heavy metal-passivated method, the method include：By the simple bud described in claim 1
Spore bacillus (Bacillus simplex), and/or the microbial inoculum in claim 2-4 described in any one is added to
In environment containing heavy metal, it is passivated with the heavy metal in environment.
8. method according to claim 7, wherein, the heavy metal is cadmium.
9. the method according to claim 7 or 8, wherein, the ring containing heavy metal pollution
Border includes soil or water body.
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| CN114164139A (en) * | 2021-10-27 | 2022-03-11 | 福州大学 | Alkali-resistant chromium-resistant bacillus cereus and application thereof |
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| CN102533601A (en) * | 2012-01-05 | 2012-07-04 | 陕西延长石油(集团)有限责任公司研究院 | Bacillus simplex, and culture method and application thereof |
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| CN102533601A (en) * | 2012-01-05 | 2012-07-04 | 陕西延长石油(集团)有限责任公司研究院 | Bacillus simplex, and culture method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114164139A (en) * | 2021-10-27 | 2022-03-11 | 福州大学 | Alkali-resistant chromium-resistant bacillus cereus and application thereof |
| CN114164139B (en) * | 2021-10-27 | 2023-03-10 | 福州大学 | Alkali-resistant chromium-resistant bacillus cereus and application thereof |
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