CN106496304B - The design of polypeptide aglucon and application in conjunction with CSFV E 2 protein specific region - Google Patents
The design of polypeptide aglucon and application in conjunction with CSFV E 2 protein specific region Download PDFInfo
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- CN106496304B CN106496304B CN201611004959.8A CN201611004959A CN106496304B CN 106496304 B CN106496304 B CN 106496304B CN 201611004959 A CN201611004959 A CN 201611004959A CN 106496304 B CN106496304 B CN 106496304B
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- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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Abstract
The present invention relates to the design of polypeptide aglucon and applications in conjunction with CSFV E 2 protein specific region, belong to polypeptide design and virus antigen detection field.Wherein, the polypeptide is FYKRTSSS with basic sequence.The present invention is by means of molecular docking and virtual screening technology, on the basis of flaviviridae E2 albumin crystal structure, pass through molecular docking technology, search in virtual peptide library with target proteins binding pattern and the optimal polypeptide ligand of affinity, finally obtaining can be with the polypeptide aglucon in conjunction with CSFV E 2 protein, its polypeptide is FYKRTSSS, i.e. Pep1 with basic sequence.The equilibrium dissociation constant KD to interact between Pep1 sequence and the CSFV E2 albumen manually expressed is 4.72 × 10‑6M, i.e., 4.72 μM, illustrate that affinity is preferable.The Pep1 sequence that the present invention designs and artificial infection CSFV, artificial expression CSFV E2 albumen can combine, and except higher outer with BVDV E2 albumen reactivity, intercrossing does not occur with other virus proteins and react, specific preferable.
Description
Technical field
The present invention relates to the design of polypeptide aglucon and applications in conjunction with CSFV E 2 protein specific region, belong to polypeptide design
And virus antigen detection field.
Background technique
Virtual screening technology based on molecular docking is interacted between one kind emerging research polypeptide and protein
Technological means.This technology mainly uses computer rapid computations to realize polypeptide and corresponding target proteins on space conformation
Docking, the molecule in virtual polypeptide database is docked with the given activity site of target proteins crystal structure one by one, is led to
Cross computer rapid computations and constantly position of the adjustment polypeptide in conjunction with target proteins, conformation, the rotatable key of intramolecule two
The amino acid residue side and skeleton of face angle and target proteins, find peptide molecule and target proteins are best on space structure
Conformation, and predict binding pattern between the two and affinity is picked out by score value close to native conformation and target egg
A kind of method of theoretical modeling intermolecular interaction of the white optimal polypeptide ligand of affinity.
Swine fever virus (Classical Swine Fever Virus, CSFV) is that swinery is caused to break out hog cholera, China
The referred to as main pathogen of rinderpest.It is that high fever, hemorrhagic focus and leukopenia occur as main feature using the swinery that falls ill
A kind of high incidence and the death rate deadly infectious disease.The virus is widely distributed in worldwide, to the cultivation in the whole world
Industry brings strong influence and loss.CSFV is one of flaviviridae, the member of pestivirus, with bovine viral diarrhea virus
(Bovine Viral Diarrhea Virus, BVDV) has very high similitude in structure.CSFV belongs to the list for having cyst membrane
Stock positive chain RNA virus, genome are about 12.3kb, formed a big open reading frame (open reading frame,
ORF), a polyprotein being made of 3989 amino acid is encoded.E2 albumen is a kind of cyst membrane for being typically located at transmembrane region
Glycoprotein plays vital effect during viruses adsorption and intrusion host cell.Meanwhile it exempts from as CSFV
Epidemic disease immunodominant proteins, the neutralizing antibody that body can be induced to generate high titre is horizontal, so that velogen strain sense can be resisted by having body
The ability of dye is to develop the preferred target protein for being directed to CSFV new generation vaccine, therefore E2 albumen also becomes many researchers
Main study subject.
Summary of the invention
The present invention is by means of molecular docking and virtual screening technology, on the basis of flaviviridae E2 albumin crystal structure
On, by molecular docking technology, search in virtual peptide library with target proteins binding pattern and the optimal polypeptide aglucon of affinity,
Its polypeptide is FYKRTSSS, i.e. Pep1 with basic sequence.Artificial synthesized Pep1 carries out table using the CSFV E2 albumen of expression and purification
Surface plasma resonance (Surface Plasmon Resonance, SPR) is tested in conjunction with ELISA, the results showed that, it is artificial to close
At Pep1 and CSFV E2 albumen have good binding ability, thus prove, by the polypeptide aglucon that designs of the present invention,
It can be used for carrying out CSFV antigen quick detection qualitatively and quantitatively.
To achieve the goals above, the technical scheme adopted by the invention is that:
Polypeptide aglucon in conjunction with CSFV E 2 protein specific region, the polypeptide are FYKRTSSS with basic sequence.
The polypeptide aglucon in conjunction with CSFV E 2 protein specific region, including matching basic sequence with the polypeptide
For core, any corresponding adjustment or modification carried out to the polypeptide with basic sequence；Decorative material includes but is not limited in nanometer
Material, fluorescent material, enzyme, biotin and specific protein.
The polypeptide is used for the identification of the E2 albumen of swine fever virus or bovine viral diarrhea virus with basic sequence,
In including but not limited to enzyme-linked immunosorbent assay (ELISA) detection.
Polypeptide aglucon described in a kind of is in the quick detection of swine fever virus or bovine viral diarrhea virus E2 albumen
Using.
Polypeptide aglucon described in a kind of is carrying out the application in qualitatively and quantitatively detection to CSFV antigen.
Beneficial effects of the present invention:
1, the present invention is by means of molecular docking and virtual screening technology, in the base of flaviviridae E2 albumin crystal structure
On plinth, by molecular docking technology, searches in virtual peptide library and match with target proteins binding pattern and the optimal polypeptide of affinity
Body, finally obtaining can be FYKRTSSS, i.e. Pep1 with basic sequence with the polypeptide aglucon in conjunction with CSFV E 2 protein, polypeptide.
Artificial synthesized Pep1, and affinity identification, Pep1 sequence and the CSFV E2 albumen manually expressed are carried out with CSFV E 2 protein
Between the equilibrium dissociation constant KD that interacts be 4.72 × 10-6M, i.e., 4.72 μM, illustrate that affinity is preferable.
2, due to the limitation of swine fever virus purification technique, it is difficult for the antibody of swine fever virus, present invention design
Obtained Pep1 sequence avoids this problem well, realizes rapid artificial synthesis and cheap testing cost.
3, the Pep1 sequence of the invention designed and artificial infection CSFV, artificial expression CSFV E2 albumen can be tied
It closes, except higher outer with BVDV E2 albumen reactivity, intercrossing does not occur with other virus proteins and react, it is specific preferable.
4, the present invention has simple, quick and at low cost feature compared with the screening of traditional phage polypeptide library；Pass through meter
Calculation machine auxiliary implements molecular docking, can provide preferable theoretical direction to realize that CSFV E 2 protein structure function parses.
5, the present invention is immunized to have compared with obtaining for the process of protein antibodies with the albumen of artificial expression and purification
It is easy to operate, time saving and energy saving, the advantages such as expense is low；By being marked screening Pep1 sequence, it can be achieved that swine fever virus E2
Albumen carries out qualitative and quantitative quick detection.
Detailed description of the invention
Fig. 1 is that Pep1 sequence is shown with the result of docking of CSFV E2 albumen.
Fig. 2 is the SPR affinity qualification result of Pep1 sequence and artificial expression CSFV E2 albumen.Wherein, ordinate represents
Signal value detected by sensor；The time that abscissa representative sample interacts in the sensor.In figure, from top to bottom respectively
The corresponding Pep1 solution concentration of curve gradually decreases.
Fig. 3 is the ELISA qualification result of Pep1 sequence and artificial expression CSFV E2 albumen.
Fig. 4 is the ELISA qualification result of Pep1 sequence and artificial infection CSFV.
Specific embodiment
Specific embodiments of the present invention will be described in further detail with reference to embodiments.
1 molecular docking of embodiment and the screening in virtual peptide library
1, the preparation of E2 albumen
The crystal structure (4JNT) that flaviviridae E2 albumen is searched from PDB database, by computer program pair
Crystal structure is analyzed, and selectes its 800 to 900th amino acid residue as docking setting regions, to carry out molecular docking.
2, the design of virtual peptide library
The space structure for establishing different aminoacids residue is realized and is criticized to input target polypeptides by computer program
Amount generates, to meet the automatic calling and processing of molecular docking computer program.Virtual peptide library is generated with linear form, no
Any side chain and head and the tail amino and carboxyl are modified.The generation of single virtual peptide library is to be no more than four amino acids residues
It is advisable.
3, judging for result is docked
Polypeptide and protein bound free energy, hydrogen chain are calculated separately, the mechanics parameters such as van der Waals carry out Comprehensive Assessment, with
This determines the selection result, and screens and obtain Pep1, and polypeptide sequence is phenylalanine-tyrosine-Lys-Arg-Soviet Union
Propylhomoserin-Ser-Ser-serine (Phe-Tyr-Lys-Arg-Thr-Ser-Ser-Ser writes a Chinese character in simplified form FYKRTSSS) (SEQ
ID NO.1), the interaction position result docked with CSFV E2 albumen is shown in Fig. 1.
2 Pep1 sequence of embodiment and the affinity of artificial expression CSFV E2 albumen are identified
1, the CSFV E2 albumen of artificial expression and purification is diluted to 1 μ g/ml (protein content using PBS buffer solution (pH 7.4)
Meter), using active ester method, respectively by 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide/n-hydroxysuccinimide
The injection of (EDC/NHS, 1:1), CSFV E2 albumen is equipped in the SPR detector of amino chip, guarantees EDC/NHS and CSFV E2
Albumen with amino chip interaction 5min, implements the coupling of CSFV E2 albumen and amino chip respectively.It, should after the completion of coupling
Sensor may be used for the interaction between measurement CSFV E2 albumen and Pep1 sequence.
2,250 μ l PBS buffer solution (pH 7.4) are injected, into sensor with maximum flow rate (150 μ l/min) runtime buffer
Liquid reaches signal base line, the flow velocity of buffer is down to 20 μ l/min, to obtain relatively stable baseline.
3, Pep1 dry powder that is artificial synthesized and modifying in aminoterminal biotinylation is used into PBS buffer solution (pH 7.4) respectively
It is dilute at concentration be 187.185 μM, 93.5925 μM, 46.7962 μM, 23.3981 μM, 11.699 μM, 5.8495 μM, 0.7311 μM
Pep1 solution inject sample every time and be all made of 20 since the Pep1 solution for successively injecting 250 μ l low concentration into sensor
The flow velocity of μ l/min and with sensor interact 5min, finally with PBS buffer solution (pH 7.4) rinse 5min.With what is obtained
The combination of various concentration Pep1 solution and CSFV E2 protein-interacting and dissociation curve are foundation, carry out Pep1 sequence with
The affinity analysis that CSFV E2 albumen combines (see Fig. 2).
The result shows that Pep1 sequence has in conjunction with preferable compatibility with the CSFV E2 albumen manually expressed, between the two
The equilibrium dissociation constant KD of interaction is 4.72 × 10-6M, i.e., 4.72 μM.
The ELISA of 3 Pep1 sequence of embodiment and artificial expression CSFV E2 albumen is identified
1, the CSFV E2 albumen of artificial expression and purification is subjected to ELISA Plate coating with 1 μ g/ml (albumen meter)；With same
Mode is by the albumen of different virus expression and purification, i.e. bovine viral diarrhea virus E2 albumen (BVDV-E2), encephalitis B virus E2 albumen
(JEV-E2), 2% ox blood of circovirus Cap protein (PCV-Cap), Pseudorabies virus gE albumen (PRV-gE) and mass fraction
Pure albumen (BSA), PBS buffer solution carry out ELISA Plate coating, as control.Wherein, envelope antigen is all made of carbonate (CBS)
Buffer is diluted, and every 50 μ l of hole is added in 96 hole elisa Plates, under the conditions of being placed in 4 DEG C overnight, is washed 5 times with PBST buffer
It is closed again with the BSA liquid of mass fraction 2% afterwards.
2, PBS buffer solution (pH 7.4) is used to dilute in Pep1 dry powder that is artificial synthesized and modifying in aminoterminal biotinylation
At the concentration of 500ng/ml, it is added in above-mentioned ELISA Plate with the volume of every 50 μ l of hole, after mixing, is placed under the conditions of 37 DEG C, keeps away
Light incubates 30min.
3, it is washed 5 times with PBST buffer, and dries the liquid in ELISA Plate hole；It is peppery using the dilution coupling of PBST buffer
The Streptavidin (1:1000) of root peroxidase is added in the ELISA Plate of drying with the volume of every 50 μ l of hole, after mixing, is set
Under the conditions of 37 DEG C, Incubation in dark 30min.
4, according to test aequum, TMB developing solution is added in above-mentioned ELISA Plate with the volume of every 100 μ l of hole, sufficiently
After mixing 30s, under room temperature, develop the color 10min.
5, the sulfuric acid terminate liquid of 2M is added in above-mentioned ELISA Plate with the volume of every 50 μ l of hole, after mixing well 30s,
On microplate reader device, light absorption value of each hole at 450nm is read, determines result.
The result shows that Pep1 sequence and artificial expression CSFV E2 albumen have preferable compatibility and specific binding, remove
It is higher outer with the cross reaction of bovine viral diarrhea virus E2 albumen, it does not react with other virus proteins (see Fig. 3).
The ELISA of 4 Pep1 sequence of embodiment and artificial infection CSFV are identified
(1) the PK-15 cell culture fluid for being inoculated with CSFV is subjected to ultrasonication, then with 200TCID50(viral level meter)
Carry out ELISA Plate coating；In the same way by different virus culture solution, i.e. bovine viral diarrhea virus (BVDV), encephalitis B virus
(JEV), the PK-15 cell of the non-virus inoculation of circovirus (PCV), the culture solution of Pseudorabies virus (PRV) and equal volume
Culture solution carries out ELISA Plate coating, as control.Wherein, envelope antigen is all made of CBS buffer and is diluted, with every 50 μ l of hole
Volume be added in 96 hole elisa Plates, under the conditions of being placed in 4 DEG C overnight, again with mass fraction 2% after wash 5 times with PBST buffer
BSA fluid-tight close.
(2) use PBS buffer solution (pH 7.4) dilute in Pep1 dry powder that is artificial synthesized and modifying in aminoterminal biotinylation
It is interpreted into the concentration of 500ng/ml, is added in above-mentioned ELISA Plate with the volume of every 50 μ l of hole, after mixing, is placed under the conditions of 37 DEG C,
Incubation in dark 30min.
(3) it is washed 5 times with PBST buffer, and dries the liquid in ELISA Plate hole；It is diluted and is coupled using PBST buffer
The Streptavidin (1:1000) of horseradish peroxidase, every 50 μ l of hole are added in the ELISA Plate of drying, after mixing, are placed in 37 DEG C
Under the conditions of, Incubation in dark 30min.
(4) according to test aequum, TMB developing solution is added in above-mentioned ELISA Plate with the volume of every 100 μ l of hole, sufficiently
After mixing 30s, under room temperature, develop the color 10min.
(5) the sulfuric acid terminate liquid of 2M is added in above-mentioned ELISA Plate with the volume of every 50 μ l of hole, after mixing well 30s,
On microplate reader device, light absorption value of each hole at 450nm is read, determines result.
The result shows that there is preferable compatibility and specificity to tie for Pep1 sequence and the CSFV cell culture fluid of artificial infection
It closes, in addition to having cross reaction higher with bovine viral diarrhea virus cell culture fluid, does not occur with other virus-culturing fluids anti-
It answers (see Fig. 4).
SEQUENCE LISTING
<110>Henan Academy of Agricultural Sciences
<120>design of polypeptide aglucon and application in conjunction with CSFV E 2 protein specific region
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 8
<212> PRT
<213>artificial sequence
<400> 1
Phe Tyr Lys Arg Thr Ser Ser Ser
1 5
Claims (5)
1. the polypeptide aglucon in conjunction with CSFV E 2 protein specific region, which is characterized in that the polypeptide is with basic sequence
FYKRTSSS。
2. the polypeptide aglucon according to claim 1 in conjunction with CSFV E 2 protein specific region, which is characterized in that packet
It includes using the polypeptide with basic sequence as core, the corresponding modification that basic sequence is carried out is matched to the polypeptide；Decorative material is nanometer
Material, fluorescent material, enzyme, biotin.
3. the polypeptide aglucon according to claim 1 in conjunction with CSFV E 2 protein specific region, which is characterized in that will
The polypeptide is used for the identification of the E2 albumen of swine fever virus or bovine viral diarrhea virus with basic sequence, wherein identification method
For enzyme-linked immunosorbent assay detection.
4. a kind of polypeptide aglucon as described in any one of claims 1-3 is in preparation swine fever virus or bovine viral diarrhea virus
The quick detection reagent of E2 albumen or the application in kit.
5. a kind of polypeptide aglucon as described in any one of claims 1-3 is quantified in preparation CSFV antigen or qualitative detection is tried
Application in agent or kit.
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| CN108383895B (en) * | 2018-05-03 | 2021-04-27 | 河南省农业科学院 | Affinity peptide capable of being combined with classical swine fever virus E2 protein and application thereof |
| CN108586578B (en) * | 2018-05-03 | 2021-04-23 | 河南省农业科学院 | Polypeptide for inhibiting hog cholera virus infection activity and application thereof |
| CN112625091B (en) * | 2020-12-25 | 2023-03-31 | 龙湖现代免疫实验室 | Polypeptide sequence combined with hog cholera virus Erns protein and application |
| CN113061161B (en) * | 2021-04-02 | 2023-09-12 | 河南省农业科学院动物免疫学重点实验室 | Inhibitory peptide ligand of targeting amyoid-beta structure and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102798708A (en) * | 2012-08-23 | 2012-11-28 | 中国科学院长春应用化学研究所 | Method for detecting binding specificity between ligand and target and drug screening method |
-
2016
- 2016-11-15 CN CN201611004959.8A patent/CN106496304B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102798708A (en) * | 2012-08-23 | 2012-11-28 | 中国科学院长春应用化学研究所 | Method for detecting binding specificity between ligand and target and drug screening method |
Non-Patent Citations (4)
| Title |
|---|
| comparison of two docking methods for peptide-protein interactions;Qiuying Yu et al;《J Sci Food Agric》;20080226;第98卷;3722-3727 * |
| 基于分子对接方法的多肽配基设计;王方雨;《2016第七届国际DNA和基因组活动周--2016第六届国际分子与细胞生物学大会、2016第一届国际遗传学大会会刊》;20160425;401 * |
| 基于分子表面形状的分子对接与虚拟高通量筛选方法的研究;徐佳维;《万方数据知识服务平台》;20051116;第6页第2段-第14页第3段 * |
| 猪瘟病毒E2基因噬菌体展示多肽库的构建及表位研究;徐和敏 等;《中国优秀硕士学位论文全文数据库 》;20070515(第5期);第2页，第5页第3段-第7页第3段，第15页第3段 * |
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