CN106490449A - A kind of utilization procyanidin suppresses Fusarium spp. to produce the methods and applications of deoxynivalenol toxin - Google Patents
A kind of utilization procyanidin suppresses Fusarium spp. to produce the methods and applications of deoxynivalenol toxin Download PDFInfo
- Publication number
- CN106490449A CN106490449A CN201610882472.3A CN201610882472A CN106490449A CN 106490449 A CN106490449 A CN 106490449A CN 201610882472 A CN201610882472 A CN 201610882472A CN 106490449 A CN106490449 A CN 106490449A
- Authority
- CN
- China
- Prior art keywords
- procyanidin
- toxin
- fusarium spp
- produce
- deoxynivalenol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses one kind procyanidin suppresses Fusarium spp. to produce the methods and applications of deoxynivalenol (DON) toxin.Utilization procyanidin proposed by the present invention suppresses Fusarium spp. to produce the methods and applications of deoxynivalenol toxin, which adds procyanidin according to amount proposed by the present invention, Fusarium spp. 30%～80% can be reduced and produce poison amount, which can reduce the accumulation of toxin, so as to ensure the safety of food.
Description
Technical field：
The invention belongs to field of food safety, and in particular to a kind of rotten using procyanidin suppression Fusarium spp. generation deoxidation snow
The methods and applications of Fusarium spp. enol toxin.
Background technology：
Grain and fruit are the Main Foods sources that people depend on for existence.Recently as the raising of logistics preservation technique,
Grain and fruit are able to transport at a distance to huge numbers of families, but from harvesting, transport, shelf sale until the logistics of consumer
During, the mechanical damage that is vulnerable to adds high temperature, high humidity and the high carbon dioxide of storage environment, easily grows pathogen.And
And, food in secondary processing process, squeeze the juice by such as vegetable-pickling, fruit, also easily grows pathogen.These pathogen except
Cause food spoilage, can also produce mycotoxin.Mycotoxin common at present has：Aflatoxin, fumonisin, Semen Maydiss are red
Mould ketenes, Trichothecenes, ochratoxin etc..They can cause serious pathological reaction to human body, in animal experiment
The middle intake for finding micro toxin for a long time has carcinogenic, teratogenesis phenomenon.
Fusarium (Fusarium) is widely distributed in grain and fruit pathogenic fungi and affects huge class funguses.
The Fusorium moniliforme Sheldon (F.moniliforme) for causing bakanae disease of rice, dry rot of potato is common are in grain, causes Semen Tritici aestivi red
F.graminearum schw (F.graminearum) of mildew etc..And banana blight is caused by Fusarium oxysporum (F.oxysporum),
Banana crown rot is then by Fusorium moniliforme Sheldon (F.moniliforme), sub- viscous group Fusorium moniliforme Sheldon (F.moniliforme
Var.subglutinans) caused by F.semitectum (Fusarium semitectum), double born of the same parents' fusariums (F.dimerum).Layer goes out
Fusarium spp. (F.proliferatum), is separated by carambola, and is found to infect various crop, and the bacterium has Fusarium
Typical case produces malicious feature, and the toxin that can be produced has：Fumonisin, Trichothecenes toxin, 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone etc. are main
Fusarium toxin.Wherein deoxynivalenol (deoxynivalenol, DON) belongs to Trichothecenes, and it is to people
Or animal immune is inhibited, the lighter can cause to vomit phenomenon, and severe one damages hemopoietic system (Wayne
L.Bryden.Mycotoxin contamination of the feed supply chain:Implications for
animal productivity and feed security.Animal feed science and technology,
2012,173(1-2):134-158).
For controlling disease, increasing antibacterial and antibacterial are put into use.For example：Propanoic acid, propionate, different bacterium
Urea, probenazole, sulfur dioxide.Especially in less developed country or area, fall as Cold Chain Logistics technology, equipment and condition are relative
Afterwards, manufacturer is partial to from cheap anti-corrosive fresh-keeping medicament such as Prochloraz, carbendazim, and these antibacterial have certain toxicity；
On the other hand, antibacterial is widely used so that pathogenic bacteria gradually develops immunity to drugs, the escalated dose thus people have to.One is not
It is that sterilization does not thoroughly cause funguses resistance to be activated to hold the consequence that ignores, and promotes Virulence-associated genes expression, toxin growing amount
And then increase substantially.Additionally, environment stress, such as：Oxidative stress and sour environment can also promote the generation of toxin.Therefore kill
Microbial inoculum improper use is not only unable to effective control disease, and hides some dangers for for food safety.
In recent years, with the attention rate more and more higher that people are safe to food, either domestic still in the world, to poison
Have strict limitation regulation.In terms of the export trade, highest extremely harsh, the food of a large portion of limitation of mycotoxin
Product trade loss is caused because toxin is exceeded, so how to reduce the yield of mycotoxin, quilt while prevention and control fungal infection
It is considered as the most important thing of food industries.The current measure for suppressing food toxin accumulation has：(1) primary prevention, sprays bactericidal
Medicine；(2) mid-term is removed, and clears up bad fruit disease fruit in time；(3) later stage chemical degradation, mechanical degradation or purification, for example with
Aluminosilicate, activated carbon and Polymer adsorption toxic substance.But once toxin is present in a large number, existing method is difficult which is thorough
Bottom removes.Thus reduce measure predominantly above-mentioned (1) and (2) of toxin accumulation.Thus research and development reduce the accumulation of toxin, edible peace
Entirely, the toxin scavenger that can improve food quality again is significant.
Procyanidin (procyanidins) is extracted from Fructus Crataegi and Pericarpium Vitis viniferae, Semen Vitis viniferae earliest, can be produced after heating
One class polyphenolic substance of raw anthocyanidin.In the plant tissues such as Lycium ruthenicum Murr., seed of Fructus Hippophae, Masson Pine Bark, litchi rind later
It was found that with the presence of procyanidin.The procyanidin (Oligimers procyanidolic, OPC/PCO) of wherein oligomer is by youngster
Theine and epicatechin condensation are formed, and usually two arrive the tetramer.OPC is due to containing multiple phenolic hydroxyl groups, can release after oxidized
Hydrion is released, thus with good antioxidant activity, about the 50 of vitamin E times.Studies have found that, carry in fruit
The procyanidin for taking can improve the activity of superoxide dismutase and glutathion peroxidase in animal body, to free radical
There is good Scavenging activity.Meanwhile, the material has no obvious toxic-side effects to human body, has been commonly used for medicine, cosmetic at present
In the middle of product, health product, play effect (Zhang Yan, the Wu Xiuxiang such as scavenging activated oxygen, defying age, antitumor, antibacterial.Procyanidin grinds
Study carefully progress.Pharmacology and Clinics of Chinese Materia Medica, 2011,27 (6)：112-115).Additionally, OPC can be used as a kind of natural safe preservative
For the fresh-keeping of food.OPC is dark red powder, soluble in water, and high temperature is degradable, stable below 40 DEG C, and its light is stable
Property is poor, and Vc and sodium sulfite can improve the stability of OPC.
The structural formula of procyanidin is as shown in formula I：
Content of the invention：
First purpose of the present invention is to provide a kind of procyanidin and is suppressing Fusarium spp. to produce deoxynivalenol bacterium alkene
Application in alcohol (DON) toxin.
Second object of the present invention is to provide procyanidin and is removing or degrading in deoxynivalenol toxin
Application.Concrete offer procyanidin of the invention produces the preparation of deoxynivalenol toxin suppression Fusarium spp. is prepared
In application.
Third object of the present invention is to provide a kind of using procyanidin suppression Fusarium spp. generation deoxynivalenol bacterium
The method of enol toxin, Fusarium spp. is inoculated in the culture medium for be added with procyanidin and is cultivated.
Preferably, described Fusarium spp. is fusarium prolifertum.
Fusarium prolifertum of the present invention, is selected to cause plurality of cereals, the pathogenic bacterium layer of fruit morbidity to go out reaping hook
Bacterium.
Preferably, the described culture medium for being added with procyanidin is to the addition of the former cyanine that concentration is 0.05～1.0g/L
The Czapek's medium of element.Czapek's medium of the present invention is Cha Shi solid mediums and Cha Shi fluid mediums.
Vc can be added according to practical situation and Ve is used in mixed way, to increase effect.
The invention has the beneficial effects as follows：Utilization procyanidin proposed by the present invention suppresses Fusarium spp. to produce deoxynivalenol
The method of bacterium enol toxin, which adds procyanidin according to amount proposed by the present invention, can reduce Fusarium spp. 30%～80%
Poison amount is produced, which can reduce the accumulation of toxin, so as to ensure the safety of food.
Description of the drawings：
Fig. 1 is impact situations of the OPC of embodiment 1 to fungal colony growth；
Fig. 2 is impact records of the OPC of embodiment 1 to fungal colony growth size；
Fig. 3 is inhibitory effects of the OPC of embodiment 1 to fungi poison.
Specific embodiment：
Following examples are that the present invention is further illustrated, rather than limitation of the present invention.
Embodiment 1：Procyanidin A2Preparative separation and Structural Identification.
1st, material：
Funguses：Fusarium prolifertum (F.proliferatum), is separated and carried out by carambola form and Molecular Identification, finds energy
Infect various plants.
Potato dextrose agar (Potato dextrose agar medium, PDA)：Take peeled potatoes
200g, is cut into small pieces, and the 1000mL that adds water boils 30min, filters off potato ball, filtrate is complemented to 1000mL, plus glucose 20g
With agar 15g, 121 DEG C of sterilizing 20min after subpackage are dissolved.
Cha Shi solid mediums (Czapek ' s agar medium, CA)：Sodium nitrate 3.0g/L, dipotassium hydrogen phosphate 1.0g/
L, magnesium sulfate (MgSO4·7H2O) 0.5g/L, potassium chloride 0.5g/L, ferrous sulfate 0.01g/L, sucrose 30g/L, agar 20g/L,
Mentioned component is soluble in water by its content, in 121 DEG C of 20min that sterilize.
Cha Shi fluid mediums (Czapek ' s broth medium, CB)：Sodium nitrate 3.0g/L, dipotassium hydrogen phosphate 1.0g/
L, magnesium sulfate (MgSO4·7H2O) 0.5g/L, potassium chloride 0.5g/L, ferrous sulfate 0.01g/L, sucrose 30g/L, by mentioned component
Soluble in water by its content, in 121 DEG C of 20min that sterilize.
2nd, processing mode：
Fusarium prolifertum is inoculated with after PDA, 28 DEG C are cultivated 7 days, stand-by with activated spawn.
The CA of high temperature sterilize is positioned in superclean bench, less than 60 DEG C are cooled to, respectively add 0.05g/L,
After the OPC (procyanidin) of 0.10g/L, 0.20g/L, 0.50g/L, 1.00g/L, shake up rapidly, subpackage 20mL culture medium is to often
In one culture dish, to be solidified after from PDA plate inoculation fusarium prolifertum to each CA plate, in 28 DEG C of incubators be inverted culture
7 days.
Colony edge with card punch on PDA plate plays the bacterium dish of a diameter of 5mm.Triangle in each Sheng 100mL CB
After adding the OPC of 0.05g/L, 0.10g/L, 0.50g/L, 1.00g/L in bottle (250mL) respectively, 3 bacterium dish of per bottle of inoculation, in
At 28 DEG C after vibration (120rpm) culture 7d, mycelia, the extraction identification of pending toxin is filtered to remove.
3rd, bacteriostatic impact and suppression poison affect
When cultivating 1,4,7 days, the bacterium colony situation on CA culture plates is observed, and given birth to vernier caliper measurement bacterium colony
Long size, is recorded.
The bacterium solution of 20mL is taken, after mixing layering with 20mL normal hexane, upper strata normal hexane phase is removed.Add 20mL ethyl acetate
Extract, after mixing, be centrifuged 3000g, 4 DEG C, 5min is layered.Extracted after 3 times with ethyl acetate, gained ethyl acetate phase is revolved
Steaming obtains sample, is dissolved in 100% methanol to be measured.
It is by high performance liquid chromatography-tandem mass system (AB SCIEX companies, U.S. to the quantitative analyses that sample carries out toxin
State) i.e. HPLC-MS/MS carries out.Sample (10 μ L) is injected into an Ekspert 100 performance liquid chromatographic column (C18 chromatograph
Post, 100 × 2.1mm, 3 μm of granular sizes, Thermo, USA).Using 35 DEG C of temperature, mobile phase (A：Acetonitrile and B：0.1% second
Acid).Mobile phase increased to 15% from 10% in 1 minute, increased within 1 to 7.5 minutes 65%, then declined from 7.5 to 9.5 minutes
To 10%, and continue extra 0.5 minute in the concentration, finally return back to initial condition.
The flow of pump is 0.5mL/min, with nitrogen as atomization gas under the conditions of 450 DEG C.Electron spray ionisation (ESI) is selected
Select [M-H]-.The parent ion of deoxynivalenol (DON) is set to m/z 295.1, and daughter ion is set to m/z 265.1
With 138.Mass spectral analyses condition is：Ion spray voltage -4500V, entrance potential -10V, exit potential -17V, taper hole voltage -
60V and collision energy -15/-21V, quantitative detection are limited to 0.1ng/mL.
4th, experimental result and analysis
As a result as shown in Figure 1, 2, after OPC processes CA culture medium, the colonial morphology of fusarium prolifertum and the difference of matched group
The process for having the phenomenon of slight promotion, 0.5g/L and 1.0g/L in low concentration is not significantly affected on bacterium colony.
As shown in figure 3, after OPC processes CB culture medium, producing deoxynivalenol (DON) poison to fusarium prolifertum
Element has significantly impact, and with the raising of concentration for the treatment of, its toxigenic amount is decreased obviously.Illustrate that OPC inhibits funguses
Produce the toxin.
As can be seen from the results：OPC tools are significantly reduced the effect of fungi poison, and the OPC of 1.0g/L processes fresh-keeping effect
Really best；OPC process has the higher reducing effect of concentration effect, i.e. concentration better.
Utilization procyanidin proposed by the present invention suppresses the method that Fusarium spp. produces deoxynivalenol toxin, its
Add procyanidin according to amount proposed by the present invention, Fusarium spp. 30%～80% can be reduced and produce poison amount, which can reduce poison
The accumulation of element, so that ensure the safety of food.
The method for suppressing Fusarium spp. to produce deoxynivalenol toxin above to the procyanidin that the present invention is provided
It is described in detail, the explanation of above example is only intended to help and understands the method for the present invention and its core concept, should
When pointing out, to those of ordinary skill in the art, under the premise without departing from the principles of the invention, can also be to the present invention
Some improvement and modification is carried out, these improvement and modification are also fallen in the protection domain of the claims in the present invention.
Claims (6)
1. procyanidin is suppressing Fusarium spp. to produce the application in deoxynivalenol toxin.
2. procyanidin is suppressing Fusarium spp. to produce answering in deoxynivalenol toxin according to claim 1
With, it is characterised in that：Described Fusarium spp. is fusarium prolifertum.
3. procyanidin is in the application for removing or degrade in deoxynivalenol toxin.
4. a kind of utilization procyanidin suppresses the method that Fusarium spp. produces deoxynivalenol toxin, it is characterised in that：
Fusarium spp. is inoculated in the culture medium for be added with procyanidin and is cultivated.
5. utilization procyanidin according to claim 4 suppresses Fusarium spp. to produce the side of deoxynivalenol toxin
Method,
Characterized in that, described Fusarium spp. is fusarium prolifertum.
6. the utilization procyanidin according to claim 4 or 5 suppresses Fusarium spp. to produce deoxynivalenol toxin
Method, it is characterised in that the described culture medium for being added with procyanidin is to the addition of the original that concentration is 0.05～1.0g/L
The Czapek's medium of anthocyanidin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610882472.3A CN106490449A (en) | 2016-10-09 | 2016-10-09 | A kind of utilization procyanidin suppresses Fusarium spp. to produce the methods and applications of deoxynivalenol toxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610882472.3A CN106490449A (en) | 2016-10-09 | 2016-10-09 | A kind of utilization procyanidin suppresses Fusarium spp. to produce the methods and applications of deoxynivalenol toxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106490449A true CN106490449A (en) | 2017-03-15 |
Family
ID=58294559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610882472.3A Pending CN106490449A (en) | 2016-10-09 | 2016-10-09 | A kind of utilization procyanidin suppresses Fusarium spp. to produce the methods and applications of deoxynivalenol toxin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106490449A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116023449A (en) * | 2023-01-05 | 2023-04-28 | 中国科学院华南植物园 | Fusarium fumonisin synthesis and pathogenicity related gene FpFUM21 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969838A (en) * | 2005-11-22 | 2007-05-30 | 陈祖辉 | Micro-ecological preparation for treating moniliasis |
| US20110059162A1 (en) * | 2009-09-04 | 2011-03-10 | Jess Dreher Reed | Tannin-chitosan composites |
| WO2015179851A1 (en) * | 2014-05-23 | 2015-11-26 | CAUCHON, Gregory | Coating method and materials |
-
2016
- 2016-10-09 CN CN201610882472.3A patent/CN106490449A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969838A (en) * | 2005-11-22 | 2007-05-30 | 陈祖辉 | Micro-ecological preparation for treating moniliasis |
| US20110059162A1 (en) * | 2009-09-04 | 2011-03-10 | Jess Dreher Reed | Tannin-chitosan composites |
| WO2015179851A1 (en) * | 2014-05-23 | 2015-11-26 | CAUCHON, Gregory | Coating method and materials |
Non-Patent Citations (2)
| Title |
|---|
| BIRGITTE SKADHAUGE: "The role of the barley testa layer and its flavonoid content in resistance to Fusarium infections", 《HEREDITAS》 * |
| 王宁等: "原花青素B Z 对细菌内毒素的中和作用研究", 《中国药理通讯》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116023449A (en) * | 2023-01-05 | 2023-04-28 | 中国科学院华南植物园 | Fusarium fumonisin synthesis and pathogenicity related gene FpFUM21 and application thereof |
| CN116023449B (en) * | 2023-01-05 | 2023-07-28 | 中国科学院华南植物园 | Fusarium fumonisin synthesis and pathogenicity related gene FpFUM21 and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nair et al. | Characterization and antifungal activity of pomegranate peel extract and its use in polysaccharide-based edible coatings to extend the shelf-life of capsicum (Capsicum annuum L.) | |
| Murmu et al. | The effect of edible coating based on Arabic gum, sodium caseinate and essential oil of cinnamon and lemon grass on guava | |
| Chen et al. | Ficus hirta fruits extract incorporated into an alginate-based edible coating for Nanfeng mandarin preservation | |
| Mahajan et al. | Natural coatings for shelf-life enhancement and quality maintenance of fresh fruits and vegetables—A review | |
| Ayala‐Zavala et al. | Antioxidant enrichment and antimicrobial protection of fresh‐cut fruits using their own byproducts: Looking for integral exploitation | |
| Xing et al. | Effects of chitosan-oil coating on blue mold disease and quality attributes of jujube fruits | |
| Ishkeh et al. | Lemon verbena (Lippia citrodora) essential oil effects on antioxidant capacity and phytochemical content of raspberry (Rubus ulmifolius subsp. sanctus) | |
| CN103250695B (en) | Natural plant extract rice seed embryo vigor retention agent as well as preparation method and application thereof | |
| Teng et al. | Eugenol emulsions affect the browning processes, and microbial and chemical qualities of fresh-cut Chinese water chestnut | |
| CN101199323B (en) | Formaldehyde scavenger for sleeve-fish and its application method | |
| Hosseini et al. | Effects of a rotational UV‐C irradiation system and packaging on the shelf life of fresh pistachio | |
| Velderrain-Rodríguez et al. | Technologies in fresh-cut fruit and vegetables | |
| CN105494616A (en) | Fruit and vegetable fresh-keeping agent containing wood vinegar | |
| El-Beltagi et al. | Comparison of antioxidant and antimicrobial properties for Ginkgo biloba and rosemary (Rosmarinus officinalis L.) from Egypt | |
| Nasution et al. | Characteristics of fresh-cut guava coated with aloe vera gel as affected by different additives | |
| Kahramanoğlu et al. | Biochemical composition of propolis and its efficacy in maintaining postharvest storability of fresh fruits and vegetables | |
| Badr et al. | Encapsulated Bioactive Ingredients of grape by‐products applicate in fresh‐cut fruit and juices diminished the ochratoxins | |
| Yu et al. | Effect of two mulberry (Morus alba L.) leaf polyphenols on improving the quality of fresh-cut cantaloupe during storage | |
| Cheng et al. | Chitosan–catechin coating as an antifungal and preservable agent for postharvest satsuma oranges | |
| Miranda-Castro | Application of chitosan in fresh and minimally processed fruits and vegetables | |
| CN102318673B (en) | Castanea henryi coating fresh-keeping agent | |
| Bal et al. | Effects of Chitosan Coating with Putrescine on Bioactive Compounds and Quality of Strawberry cv. San Andreas During Cold Storage. | |
| Lichanporn et al. | Effect of silver particle-longkong peel extract coating on postharvest decay and browning in longkong fruit | |
| Wen et al. | Natamycin treatment reduces the quality changes of postharvest mulberry fruit during storage | |
| CN105053169A (en) | Edible strawberry compound preservative and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170315 |
|
| RJ01 | Rejection of invention patent application after publication |