CN106483301A - Immunochromatography assay kit and method of use thereof - Google Patents
Immunochromatography assay kit and method of use thereof Download PDFInfo
- Publication number
- CN106483301A CN106483301A CN201610704258.9A CN201610704258A CN106483301A CN 106483301 A CN106483301 A CN 106483301A CN 201610704258 A CN201610704258 A CN 201610704258A CN 106483301 A CN106483301 A CN 106483301A
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- China
- Prior art keywords
- set group
- test paper
- immunochromatographiassays assays
- assays set
- engagement pad
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- 238000003556 assay Methods 0.000 title claims description 34
- 238000003149 assay kit Methods 0.000 title abstract 3
- 238000003317 immunochromatography Methods 0.000 title description 4
- 238000012360 testing method Methods 0.000 claims abstract description 47
- 238000000034 method Methods 0.000 claims abstract description 13
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims abstract description 9
- 238000003018 immunoassay Methods 0.000 claims abstract description 5
- 102000001554 Hemoglobins Human genes 0.000 claims description 21
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- 238000001514 detection method Methods 0.000 abstract description 10
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- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 8
- 239000007853 buffer solution Substances 0.000 description 8
- 239000006166 lysate Substances 0.000 description 8
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 7
- 206010012601 diabetes mellitus Diseases 0.000 description 7
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 229920000122 acrylonitrile butadiene styrene Polymers 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 5
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- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
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- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
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- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 2
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- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
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- 102000004169 proteins and genes Human genes 0.000 description 2
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- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- MNWBNISUBARLIT-UHFFFAOYSA-N sodium cyanide Chemical compound [Na+].N#[C-] MNWBNISUBARLIT-UHFFFAOYSA-N 0.000 description 2
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- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 description 1
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- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
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- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/042—Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
Abstract
The invention provides an immunochromatographic assay kit, comprising test paper and a contact pad. The test paper comprises a substrate and a film layer, wherein at least one immunoassay detection substance is arranged on the film layer. The contact pad comprises an outer film, wherein a sodium acetate solution and a core sheet are contained in the outer film. The invention also provides a using method of the immunochromatographic assay kit.
Description
Technical field
The present invention relates to a kind of immunochromatographiassays assays set group and its using method, particularly a kind of exempting from engagement pad
Epidemic disease chromatographic analysis set group and its using method.
Background technology
With biomedical prosperity, there are increasing detection disease mode, for example various biochips, ion exchange
Chromatography or immunization etc..Wherein the quickest and wide variety of be to utilize immunochromatographiassays assays
(immunochromatographic analysis) method.
Immunochromatographic method (Immunochromatography) is a kind of quick diagnosis technology that rose in recent years, and which is former
Reason is first fixed on antibody on special fiber or film, behind one end immersion sample (urine or blood), as capillary is made
With sample will be moved forward along the fiber or film, and when the region for being fixed with antibody is moved to, in sample, corresponding antigen is
Specifically bind with the antibody, as long as substance that show color is marked on antibody, just can be formed in the land macroscopic
Detection band.
However, as existing immunochromatographic method is by the undetected object chemical bond in antibody and sample, therefore existing
These products have many technology for needing and overcoming, and such as reaction temperature is often the key of impact.When immunoassay be not appropriate
Reaction temperature under when carrying out, so that reaction result is presented or recognize, this situation is especially in the concentration mistake of undetected object
It is susceptible to when in little or sample, between different undetected objects, concentration difference is too small.Therefore, it is also desirable to a kind of immunochromatography of optimization divides
Analysis set group, to solve the above problems.
Content of the invention
The invention provides a kind of immunochromatographiassays assays set group, comprising a ph test paper ph and an engagement pad.Ph test paper ph includes
One base material and a film layer, are wherein provided with least one immunoassay detecting material in film layer.Engagement pad includes an adventitia,
Adventitia inner containment has a supersaturated sodium acetate solution and a core piece.
According to another embodiment of the present invention, a kind of using method of immunochromatographiassays assays set group is additionally provided, is provided first
Aforesaid immunochromatographiassays assays set group, by recessed for the core piece of engagement pad folding, then the ph test paper ph is contacted with engagement pad, is subsequently adding
One undetected object is in ph test paper ph.
The immunochromatographiassays assays set group of the present invention, due to the engagement pad with particular design, even if in relatively low room temperature ring
Also operable under border, it can be ensured that ph test paper ph is carried out at desired temperatures, and the low cost of engagement pad and can in test when provide
Temperature that is stable and being suitable for, increased practicality and the reliability of product.
Description of the drawings
Fig. 1 and Fig. 2 is one of embodiment schematic diagram of ph test paper ph of the present invention.
Fig. 3 is a kind of embodiment schematic diagram of engagement pad of the present invention.
Fig. 4 uses a kind of embodiment schematic diagram of engagement pad collocation ph test paper ph for the present invention.
Fig. 5 is another kind of embodiment schematic diagram of engagement pad of the present invention.
Fig. 6 and Fig. 7 is another kind of embodiment schematic diagram of engagement pad of the present invention collocation ph test paper ph.
Wherein, description of reference numerals is as follows：
The 3rd area of 2 ph test paper ph 223
11 matrix, 3 engagement pad
12 film layer, 31 core piece
21 sample area, 32 adventitia
211 sample well, 33 SAS
22 interpretation area, 34 pedestal
221 first area, 4 groove
222 second area, 6 groove
Specific embodiment
For enabling those skilled in the art to be further understood that the present invention, hereafter spy describes the structure of the present invention in detail
Become content and the effect for wishing to realize.Hereafter having disclosed enough details enables person skilled in art to have to implement.
Invention broadly provides a kind of immunochromatographiassays assays set group, can not be limited by environment temperature and be used.This
The set group of invention mainly includes a ph test paper ph and an engagement pad, but treats that side sample can also include further depending on different types of
Other elements.Ph test paper ph is provided with least one immunoassay detecting material, for example comprising a matrix and a film layer in film layer
Be antibody or other and antibody there are similar structures and with narrow spectrum chemical substance.
The immunochromatographiassays assays set group of the present invention can be that any immunochromatography for checking disease or physiological status divides
Analysis set group, preferably needs can have the immunochromatographiassays assays set of optimum detection quality in higher temperature (for example Celsius more than 30 degree)
Group, such as diabetes immunochromatographiassays assays set group, cancer immunity chromatographic analysis set group, hepatitis immunochromatographiassays assays set group etc., on
State individuality and human body is not limited to, and the inspection of various Animal diseases can also be included, the such as immunity of detection cat and dog heartworm disease
Chromatographic analysis set group etc..Examples below with detect diabetes immunochromatographiassays assays set group as an example.
The immunochromatographiassays assays set group of the detection diabetes of the present embodiment includes ph test paper ph, engagement pad, lysate, buffering
Liquid, adopt inspection thing and micropipette.The ph test paper ph of the present embodiment is mainly by detecting the dense of candy base hemoglobin in blood
Degree is to judge blood sugar concentration.Fig. 1 and Fig. 2 is refer to, show one of embodiment schematic diagram of one ph test paper ph of the present invention.
As shown in Figures 1 and 2, ph test paper ph 2 is included：One matrix 11, and the film layer being arranged in matrix (substrate) 11
(layer)12.Its mesostroma 11 can be a rigid substrate or the matrix of an easy song, and the matrix of wherein hard is, for example, one
Glass matrix (glass substrate) or a silicon matrix (silicon substrate), and the matrix of Yi Quxing for example includes
There is dimethyl silicone polymer (polydimethylsiloxane；PDMS), polystyrene (polystyrene), polypropylene
(polypropylene), polymethyl methacrylate (polymethylmethacrylate), Merlon
(polycarbonate), polyisobutene (polyisobutylene) and combination is allowed to, but is not limited thereto.Film layer 12 is arranged
In the one side of matrix 11, film layer 12 has a sample area 21 and an interpretation area 22, and sample area 21 and interpretation area 22 are to set respectively
Put in film layer 12.Sample area 21 has a sample well 211, is available for sample and contacts in every way and enter in sample area 21,
Such as instill or be stained with leaching.Interpretation area 22 is combined in this area with determinand with an opening, antibody, in the present embodiment, interpretation area 22
With one first area 221, one second area 222 and one the 3rd area 223, can be combined with antibody that is identical or differing respectively.
In the present embodiment, the 3rd area 223 combines one first anti-candy base hemoglobin antibodies (first anti-glycosylated
Hemoglobin antibody), which is in order to combine candy base hemoglobin (HbA1c) in blood sample；Second area 222 ties
Unify first anti-hemoglobin antibodies (first anti-hemoglobin antibody), which is in order to combine in blood sample not
Hemoglobin (Hb) by candy base；First area 221 combines an AIA (anti-IgG antibody), its
In order to combine an immune globulin antibody (IgG antibody), using as a tester (control).When the blood red egg of candy baseization
White or do not entered after ph test paper ph by sample area 21 by the hemoglobin of candy base, you can respectively with interpretation area 22 in the firstth area
221st, the antibody in the second area 222 or the 3rd area 223 is combined, then by the second anti-hemoglobin antibodies (energy with gold grain
Be incorporated into candy base hemoglobin can also be incorporated into not by the hemoglobin of candy base) with candy base hemoglobin or not by candy base
The hemoglobin of change is combined, and produces the different figure line of the depth.In the preferred embodiment of the present invention, gold grain is nm of gold
Grain.
In the present embodiment, may compare aforementioned the second anti-hemoglobin antibodies with nanogold particle to combine not by candy base
The figure line (afterwards be referred to as Hb figure line) presented during hemoglobin (Hb) of change is second anti-blood red with nanogold particle with aforementioned
Protein antibodies combine the shade of the presented figure line of candy base hemoglobin (HbA1c) (being referred to as HbA1c figure line afterwards), reach
Judge the purpose of candy base HC in blood sample.By adjusting the first anti-candy base hemoglobin antibodies and first
Anti- hemoglobin antibodies and the quantity of the with nanogold particle second anti-hemoglobin antibodies, can make Hb figure line and HbA1c
The depth of the presented color of figure line is identical, and represent candy base HC value just position in a critical value.If treating test sample
The color is presented by the HbA1c figure line of product is compared with Hb figure line depth, then it represents that candy base HC value is higher than critical value, and
Critical value then can be set as any to monitoring the significant numerical value of blood sugar concentration.
The lysate of the immunochromatographiassays assays set group of the detection diabetes of the present embodiment be in order to molten schizocyte, lysate
It can be any lysate in order to molten schizocyte.For example, a kind of formula of lysate can be included:1.55 mM
(mM)/liter ammonium chloride (NH4Cl), 12 mMs (mM)/liter sodium acid carbonate (NaHCO3) and 0.1 mM (mM)/liter
Ethylenediamine tetra-acetic acid (EDTA)；In another embodiment, lysate is included:Ammonium chloride (the NH of 8.26 g (g)4Cl), 1 is public
Gram (g) saleratus (KHCO3) and 0.037 g (g) ethylenediamine tetra-acetic acid (EDTA).In another embodiment, cracking
Liquid may include:The phosphate of per liter 40 mMs (mmol/L), the sodium chloride (NaCl) of per liter 60 mMs (mmol/L), per
Rise the Cymag (NaCN) of 5 mMs (mmol/L) and the Triton X-100 (alkyl of per liter 0.5 milliliter (mL/L)
Aryl Aethoxy Sklerol；alkylaryl polyether alcohol).
The buffer solution of the immunochromatographiassays assays set group of the detection diabetes of the present embodiment is used to process sample, buffer solution bag
Include:In order to make the mixing of the same product liquid and equally distributed buffer solution, buffer solution can be various for the steady of Protein requirement
The mixing of fixed degree and promotion sample liquid and equally distributed buffer solution, for example, the buffer solution can be included:0.66 milligram/
The bovine serum albumin (BSA) for rising and the phosphate buffered saline (PBS) (phosphate-buffered of 3% polysorbas20 (Tween 20)
saline；PBS), but it is not limited with above-mentioned.
The inspection thing of adopting of the immunochromatographiassays assays set group of the detection diabetes of the present embodiment is used to collect blood sample to be measured
Product, adopt inspection thing and can adopt inspection thing and may include metal or plastics made by the various materials for being adapted to collect blood sample
Etc. material, for example, it can be the blood taking needle being made up of metal or plastics to adopt inspection thing, but is not limited with above-mentioned.
The pipette of the immunochromatographiassays assays set group of the detection diabetes of the present embodiment is used to pipette samples liquid and injects
In the sample well 211 of the ph test paper ph, pipette can be made by the material of various suitable draw blood samples, and pipette can be wrapped
Included the materials such as glass or plastics, for example, pipette can by the micropipette made by glass or plastics etc., but not with
Above-mentioned it is limited.
The engagement pad of the immunochromatographiassays assays set group of the present invention is used to carry or be covered on ph test paper ph, provides ph test paper ph
2 appropriate reaction temperatures, such as between 20 degree～60 degree Celsius, it is possible to continue for some time stable supply.Refer to Fig. 3,
It show a kind of embodiment schematic diagram of engagement pad of the present invention.As shown in figure 3, the engagement pad 3 of the present embodiment includes a core piece
31st, a sodium acetate (sodium acetate) solution 33 and an adventitia 32.Core piece 31 is with SAS 33 in adventitia 32
In.The SAS 33 of the present invention is a supersaturated solution, and its concentration is preferably greater than 120g/ml, more preferably larger than
160g/ml.In an embodiment, SAS 33 is to be dissolved in water, but it is also possible to be dissolved in other solution.Adventitia 32 is preferably
Made by soft material, for example plastic-like polymer, such as polypropylene (Polypropylene, PP), polystyrene
(Polystyrene, PS), acrylonitrile-butadiene-styrene copolymer resin (Acrylonitrile butadiene
Styrene, ABS), PET (Polyethylene terephthalate, PET), polyester resin
(Polyester, PES), polyamide (Polyamides, PA), polyvinyl chloride (Polyvinyl chloride, PVC), polyurethane
(Polyurethanes, PU), Merlon (Polycarbonate, PC), polyvinyl dichloride (Polyvinylidene
Chloride, PVDC), polyethylene (Polyethylene, PE), Merlon/ABS resin (Polycarbonate/
Acrylonitrile Butadiene Styrene, PC/ABS), polymethyl methacrylate (Polymethyl
Methacrylate, PMMA), polytetrafluoroethylene (PTFE) (Polytetrafluoroethylene, PTFE), polyether-ether-ketone
(Polyetheretherketone, PEEK), but be not limited thereto.
The using method of the engagement pad 3 of the present invention, is after folding core piece 31 is pulled, crystallizes supersaturated sodium acetate solution 33 and release
Heat release energy, and it is uniform to make engagement pad 3 reach a predetermined temperature and 32 temperature of adventitia, subsequently, as shown in figure 4, again ph test paper ph 2 is set
It is smoothed out reaction by putting in the engagement pad 3, after the completion of waiting (such as 5～10 minutes) scheduled time to confirm reaction, will
Ph test paper ph 2 removes engagement pad 3.In an embodiment, it is also possible to so that engagement pad 3 is covered on ph test paper ph 2.
Fig. 5 is refer to, which is another embodiment schematic diagram of engagement pad of the present invention.As shown in figure 5, except aforementioned core piece
31st, SAS 33 and adventitia 32, the present embodiment can also be fitted with adventitia 32 with a base 34.In an embodiment
In, base 34 can provide ph test paper ph 2 and support, preferably the material of solid.And in another embodiment, pedestal 34 is alternatively thermal conductivity
Property material, such as metal etc., now ph test paper ph 2 act on when can arrange or be in direct contact with metal base 34, with increase be heated
Uniformity.
Fig. 6 is refer to, which is another embodiment schematic diagram of engagement pad of the present invention and ph test paper ph.As shown in fig. 6, pedestal 34
Thermal conductance material is preferably comprised, and there is a groove 6, ph test paper ph 2 in groove 6, can be accommodated.Therefore when 3 heat release of engagement pad, be located at
Ph test paper ph 2 in groove 6 can be reacted in the case of more uniform temperature.
Fig. 7, one embodiment schematic diagram that shown engagement pad of the present invention is combined is refer to ph test paper ph.As shown in fig. 7, examination
The matrix 11 for testing paper 2 can accommodate engagement pad 3 with a groove 4.The position of groove 4 is designed with size visible product and is adjusted, example
As groove 4 can generally with the same length of ph test paper ph 2, and in another embodiment, the size of groove 4 can also correspond only to treat lateral areas
21 and interpretation area 22.
Experiment
Embodiment one
The immunochromatographiassays assays set group of the present embodiment includes：
Ph test paper ph, with a matrix, the matrix includes dimethyl silicone polymer (PDMS), and test strips have more a film
Layer, film layer include one first anti-hemoglobin antibodies, one first anti-candy base hemoglobin antibodies and an anti-immunity ball egg
Bai Kangti, afore mentioned antibodies are fixedly disposed at the zones of different in the matrix of afore-mentioned test paper.First anti-hemoglobin antibodies can
In conjunction with not by the hemoglobin (Hb) of candy base in blood sample, and the first anti-candy base hemoglobin antibodies then can be in conjunction with candy base
Change hemoglobin (HbA1c).As for AIA then can binding domain-immunoglobulin antibody using as a tester
(control).The present embodiment more provides a kind of the second anti-hemoglobin antibodies with nanogold particle, aforementioned with nm of gold
Second anti-hemoglobin antibodies of particle are to be similarly disposed at film layer, i.e., the first anti-hemoglobin antibodies and the first anti-candy base blood
Lactoferrin antibody arrange film layer, but aforementioned the second anti-hemoglobin antibodies with nanogold particle only arrange unlocked
In film layer.Aforementioned the second anti-hemoglobin antibodies with nanogold particle can be in combination with by the blood red egg of candy base
(Hb) and candy base hemoglobin (HbA1c) color is presented in vain.The critical value that ph test paper ph color is presented by the present embodiment is adjusted
Whole concentration when being 6.0% for HbA1c.That is, when the HbA1c concentration of sample is higher than 6.0%, the area that will be combined in the antibody
Band assumes color, and HbA1c concentration is higher than more 6.0%, then color is deeper.
Lysate：1.55 mMs (mM)/liter ammonium chloride (NH4Cl), 12 mMs (mM)/liter sodium acid carbonate
(NaHCO3) and 0.1 mM (mM)/liter ethylenediamine tetra-acetic acid (EDTA).
Buffer solution：The bovine serum albumin (BSA) of 0.66 mg/litre and the phosphate-buffered of 3% polysorbas20 (Tween 20)
Salt solution (phosphate-buffered saline；PBS).
Engagement pad：Long 10 centimeters, wide 7 centimeters, adventitia is polypropylene, the internal supersaturation acetic acid with 130g/ml concentration
Sodium solution, and a foil.During sodium acetate crystallization exotherm, which can be under 15 degree Celsius of room temperature, and providing surface temperature is
25 degree Celsius and continue more than 20 minutes.
A kind of method in order to inspect candy base hemoglobin of the present embodiment includes following steps:
A () gathers blood sample, add lysate simultaneously to measure the wherein concentration of HbA1c, pick out concentration 5.5% with
The sample of 6.5%HbA1c.
B the foil of engagement pad is pulled folding by (), and pressable engagement pad is connecing internal sodium acetate crystallization uniformly dispersing
In touch pad, then ph test paper ph is placed over contact pads.
C () utilizes micropipette pipette samples liquid, and the 10 microlitres sample liquid is injected the sample of aforesaid ph test paper ph
In product area；
D () is by be reacted in the sample area of aforesaid for 100 microlitres to 150 microlitres of the buffer solution addition ph test paper ph.
E () after pad is placed into contact with and is reacted about 5～10 minutes, it may appear that Red trace, and compares its Hb figure line
Whether concentration of specimens can really be reacted with the depth of HbA1c figure line.
Comparative example
The step of comparative example one, is roughly the same with embodiment one, arranges except comparative example one is not in contact with pad.Embodiment one
Or the experiment of comparative example one, all respectively room temperature Celsius 15 degree and Celsius 25 degree under carry out.
, as shown in following table one, wherein+expression figure line is rough visible for experimental result；++ represent that figure line is obvious；+++ represent figure line
Clearly.
Table one
Knowable to above-mentioned table one, in embodiment one, either in the environment of low temperature is Celsius 15 degree or 25 degree Celsius
Under environment, can be correctly by obvious figure line come higher HbA1c concentration in response sample.But in comparative example one,
Then have no idea under relatively low room temperature is Celsius 15 degree correct by figure line come response sample in higher HbA1c concentration.This shows
The engagement pad of the present invention can provide temperature that is stable and being suitable for reaction really, to guarantee the correctness that reacts.
In sum, the invention provides a kind of immunochromatographiassays assays set group, especially for easily affected by temperature
Immunochromatographiassays assays set group.Due to the engagement pad with particular design, even if also operable under relatively low room temperature environment, permissible
Guarantee that ph test paper ph is carried out at desired temperatures, increased practicality and the reliability of product.
The preferred embodiments of the present invention are the foregoing is only, the present invention is not limited to, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, made any repair
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of immunochromatographiassays assays set group, it is characterised in that include：
One ph test paper ph, comprising：
One matrix；And
One film layer, is provided with least one immunoassay detecting material in the film layer；And
One engagement pad, includes an adventitia, accommodates a supersaturated sodium acetate solution and a core piece in the adventitia.
2. immunochromatographiassays assays set group according to claim 1, it is characterised in that the supersaturated sodium acetate solution dense
Degree is more than 120g/ml.
3. immunochromatographiassays assays set group according to claim 1, it is characterised in that the core piece includes metal.
4. immunochromatographiassays assays set group according to claim 1, it is characterised in that the engagement pad also includes a base,
Which is attached to the adventitia.
5. immunochromatographiassays assays set group according to claim 4, it is characterised in that the base includes thermal conductivity material.
6. immunochromatographiassays assays set group according to claim 5, it is characterised in that the base includes a groove, to hold
Receive the ph test paper ph.
7. immunochromatographiassays assays set group according to claim 1, it is characterised in that set in the matrix of the ph test paper ph
A groove is equipped with, to accommodate the engagement pad.
8. immunochromatographiassays assays set group according to claim 1, it is characterised in that the film layer includes：
One first area, is provided with an AIA；
One second area, is provided with one first anti-hemoglobin antibodies；And
One the 3rd area, is provided with one first anti-candy base hemoglobin antibodies, and is additionally provided with one in the film layer with gold
Second anti-hemoglobin antibodies of grain.
9. a kind of using method of immunochromatographiassays assays set group, it is characterised in that include：
Immunochromatographiassays assays set group described in claim 1 is provided；
By recessed for the core piece of engagement pad folding；
The ph test paper ph is contacted with the engagement pad；And
Add a undetected object.
10. the using method of immunochromatographiassays assays set group according to claim 9, wherein first by after recessed for core piece folding,
Again the ph test paper ph is contacted with the engagement pad, be added followed by the undetected object.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW104128376 | 2015-08-28 | ||
| TW104128376A TW201708819A (en) | 2015-08-28 | 2015-08-28 | Immunochromatographic analysis kit and a method of using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106483301A true CN106483301A (en) | 2017-03-08 |
Family
ID=58273869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610704258.9A Pending CN106483301A (en) | 2015-08-28 | 2016-08-22 | Immunochromatography assay kit and method of use thereof |
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| Country | Link |
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| TW (1) | TW201708819A (en) |
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| CN1490407A (en) * | 2003-08-13 | 2004-04-21 | 公安部第二研究所 | Antihuman hemoglobin detection reagent strips and monoclone antibody contained therewith |
| CN101300470A (en) * | 2003-10-29 | 2008-11-05 | Mec戴内米克公司 | Micro mechanical methods and systems for performing assays |
| CN201101359Y (en) * | 2007-09-30 | 2008-08-20 | 崔玎琳 | Drinking cup capable of self-heating |
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Also Published As
| Publication number | Publication date |
|---|---|
| TW201708819A (en) | 2017-03-01 |
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Application publication date: 20170308 |