CN106480150A - A kind of separation method digesting liquid by membrance separation blood protein - Google Patents
A kind of separation method digesting liquid by membrance separation blood protein Download PDFInfo
- Publication number
- CN106480150A CN106480150A CN201611016137.1A CN201611016137A CN106480150A CN 106480150 A CN106480150 A CN 106480150A CN 201611016137 A CN201611016137 A CN 201611016137A CN 106480150 A CN106480150 A CN 106480150A
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- Prior art keywords
- eluting
- shearing
- equipment
- separation
- pulverize
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The present invention relates to a kind of separation method digesting liquid by membrance separation blood protein, belong to blood protein liquid separation technology field, this production method comprises the following steps:Step one：By whole blood, hyperglobulinemia filters, shearing smashes, after thermal denaturation shearing pulverize, after enzyme digestion reaction shearing pulverize etc. and to process；Step 2：Step one is isolated peptides supernatant and the precipitation of class containing haemachrome by ceramic membrane equipment, and the eluting peptide that adds water, from the high aperture filter material of degree of accuracy, screening is precise and appropriate, different pore size filter material can be changed according to hydrolysis degree, motility is strong, dynamically wash away eluting from high flow capacity, filter cake is avoided to pile up absorption solubility peptides, improve solubility peptides yield and settleable solids purity, the eluting eluting that can add water carries out abundant eluting to settleable solids, improve solubility peptides yield and carry out abnormal smells from the patient improvement, settleable solids are with mobility liquid output and transfer, reduce reprocessing handling process and difficulty.
Description
Technical field
Present invention seek to address that precipitability large-molecular peptides are filled with solubility peptides in blood protein hydrolysis (containing enzymolysis) liquid
Point separate, and keep precipitation to possess preferable mobility, be easy to reprocess, the problems such as separation process composite sanitary requires, belong to blood
Liquid eggs white liquor separation technical field.
Background technology
Blood enzymolysis as whole blood, blood cell deep processing the most frequently used processing method it is intended to improve protein raw materials application
It is worth and application, blood protein is because containing higher haemachrome, and destroys former globin structure after enzymolysis, and reactant liquor becomes
Furvous, are that the use of finished product brings the obstacle of color, and react and terminate destroy the enzyme treatment, macromolecular substances polyhemoglobin element,
Easily precipitate, method the most frequently used at present is plate-and-frame filtration, more various centrifugal separation methods have peptides high income, and equipment investment becomes
The features such as this is low, equipment runs simple, is the most widely used equipment of the industry, but equipment has labor intensity here simultaneously
Greatly, efficiency is low, the disadvantage such as sanitary condition difference.
Content of the invention
The present invention utilizes the peptide constituents in ceramic membrane equipment separation blood enzymolysis solution and the settleable solids containing haemachrome,
Motility is strong, dynamically washes away eluting from high flow capacity, it is to avoid absorption solubility peptides piled up by filter cake, improve solubility peptides yield
With settleable solids purity, the eluting eluting that can add water carries out abundant eluting to settleable solids, improve solubility peptides yield and
Carry out abnormal smells from the patient improvement, settleable solids, with mobility liquid output and transfer, reduce reprocessing handling process and difficulty.
A kind of separation method digesting liquid by membrance separation blood protein, this production method comprises the following steps:Step one：
By whole blood, hyperglobulinemia filters, shearing smashes, after thermal denaturation shearing pulverize, after enzyme digestion reaction shearing pulverize etc. and to process；Step
Two：Step one is isolated peptides supernatant and the precipitation of class containing haemachrome by ceramic membrane equipment, and the eluting peptide that adds water.
Furthermore, in step one, whole blood, blood cell need clipped disintegrating apparatus to filter before crossing equipment, no rigid
Grain, hair, impurity etc..
Furthermore, in step 2, reactant liquor first adjusts PH4.3～6.0, carries out separating simultaneously by ceramic membrane equipment
Add water eluting, and equipment retains the equipment matching scheme of aperture 50～500nm.
Beneficial effect of the present invention：Precise and appropriate from the high aperture filter material of degree of accuracy, screening, can be changed different according to hydrolysis degree
Aperture filter material, motility is strong, dynamically washes away eluting from high flow capacity, it is to avoid absorption solubility peptides piled up by filter cake, improve solvable
Property peptides yield and settleable solids purity, the eluting eluting that can add water carries out abundant eluting, improves solubility to settleable solids
Peptides yield and carry out abnormal smells from the patient improvement, settleable solids with mobility liquid output and transfer, reduce reprocessing handling process and
Difficulty, equipment closed system, contacting material part wholesomeness are high, meet food, medical material etc. and produce sanitary condition, if
Standby take up room little, operate labor intensity, it is possible to decrease productive labor cost more than half, service life of equipment length, consumable accessory
Few, maintaining expense bottom；Equipment can CIP on-line cleaning, reduce cleaning waste water, do not pollute adjacent workshop section working environment.
Invention implementation
The present invention will be described with reference to embodiments it will be appreciated that preferred embodiment described herein is merely to illustrate
With the explanation present invention, it is not intended to limit the present invention.
Embodiment
A kind of separation method digesting liquid by membrance separation blood protein, this production method comprises the following steps：
Step one：Blood protein is carried out processing, digests, during needed clipped disintegrating apparatus to filter, no rigid granule,
Hair, impurity etc.；
Step 2：Enzymolysis solution in step one is adjusted PH to 4.3～6.0, by film device（Film device retains aperture 50～500nm）
Isolate supernatant and settleable solids, and eluting that settleable solids are added water.
The present invention is not limited to above-mentioned preferred forms, and anyone can show that under the enlightenment of the present invention other are various
The product of form, however, making any change in its details, every have technical scheme identical or similar to the present application,
It is within the scope of the present invention.
Claims (3)
1. a kind of separation method by membrance separation blood protein enzymolysis liquid is it is characterised in that this production method includes following step
Suddenly:
Step one：By whole blood, hyperglobulinemia filters, shearing smashes, after thermal denaturation shearing pulverize, after enzyme digestion reaction shearing pulverize etc.
Process；
Step 2：Step one is isolated peptides supernatant and the precipitation of class containing haemachrome by ceramic membrane equipment, and the eluting that adds water
Peptide.
2. the step one in method according to claim 1 it is characterised in that：Whole blood, blood cell need clipped pulverizing before crossing equipment
Equipment filters, no rigid granule, hair, impurity etc..
3. the step 2 in method according to claim 1 it is characterised in that：Reactant liquor first adjusts PH4.3～6.0, by pottery
Porcelain film device is separated and is added water eluting, and equipment retains the equipment matching scheme of aperture 50～500nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611016137.1A CN106480150A (en) | 2016-11-18 | 2016-11-18 | A kind of separation method digesting liquid by membrance separation blood protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611016137.1A CN106480150A (en) | 2016-11-18 | 2016-11-18 | A kind of separation method digesting liquid by membrance separation blood protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106480150A true CN106480150A (en) | 2017-03-08 |
Family
ID=58272576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611016137.1A Pending CN106480150A (en) | 2016-11-18 | 2016-11-18 | A kind of separation method digesting liquid by membrance separation blood protein |
Country Status (1)
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| CN (1) | CN106480150A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108048502A (en) * | 2018-01-10 | 2018-05-18 | 安徽菁硕科技有限公司 | A kind of method of animal blood enzymolysis filter press cake production ferroheme |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349603A (en) * | 2015-11-19 | 2016-02-24 | 桐城市雨润生物科技有限公司 | Method for producing protein peptide and hemachrome through enzymatic hydrolysis of pig blood |
| CN105859874A (en) * | 2016-05-26 | 2016-08-17 | 陈石良 | Preparation method for producing pig haemocyte active small peptide powder through one-step method |
| CN106086142A (en) * | 2016-08-12 | 2016-11-09 | 江苏久吾高科技股份有限公司 | A kind of whole blood Gly-His-Lys extracting method and device |
-
2016
- 2016-11-18 CN CN201611016137.1A patent/CN106480150A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349603A (en) * | 2015-11-19 | 2016-02-24 | 桐城市雨润生物科技有限公司 | Method for producing protein peptide and hemachrome through enzymatic hydrolysis of pig blood |
| CN105859874A (en) * | 2016-05-26 | 2016-08-17 | 陈石良 | Preparation method for producing pig haemocyte active small peptide powder through one-step method |
| CN106086142A (en) * | 2016-08-12 | 2016-11-09 | 江苏久吾高科技股份有限公司 | A kind of whole blood Gly-His-Lys extracting method and device |
Non-Patent Citations (1)
| Title |
|---|
| 张纯丽: "酶解猪血血红蛋白制备小肽粉方法的研究", 《中国优秀硕士论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108048502A (en) * | 2018-01-10 | 2018-05-18 | 安徽菁硕科技有限公司 | A kind of method of animal blood enzymolysis filter press cake production ferroheme |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170308 |