CN106480000A - A kind of production method of magnetic immobilized enzyme - Google Patents
A kind of production method of magnetic immobilized enzyme Download PDFInfo
- Publication number
- CN106480000A CN106480000A CN201611135592.3A CN201611135592A CN106480000A CN 106480000 A CN106480000 A CN 106480000A CN 201611135592 A CN201611135592 A CN 201611135592A CN 106480000 A CN106480000 A CN 106480000A
- Authority
- CN
- China
- Prior art keywords
- immobilized enzyme
- reaction substrate
- less
- production method
- magnetic immobilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a kind of production method of magnetic immobilized enzyme, form colloidal sol step, magnetic material and enzyme inclusion step, gel step etc. including reaction substrate, achieve the production technology of magnetic immobilized enzyme, the safety that improve production, the operability improving production, simplify production technology, also make magnetic inclusion and gel evenly simultaneously, improve the stability of magnetic immobilized enzyme enzyme.
Description
Technical field
The present invention relates to field of production, particularly a kind of field of production of magnetic immobilized enzyme.
Background technology
In Chinese invention patent application CN104498472A announced on April 8th, 2015, describe a kind of magnetic and fix
Change cellobiase mesoporous nano material and preparation method thereof, the method is by being fixed of enzyme, realizing the repeatable of enzyme
Use, and improve the operability of enzyme recovery by the magnetic material of inclusion.
But in this patent of invention, and the production technology of undisclosed immobilized enzyme.Magnified to commercial production by laboratory,
It is the engineering of a complexity and arduousness, the difference of process program can cause the difference of the performance of product, and this is also industrial
Feature.The practical problem occurring in commercial production for the preparation method disclosed in patent application CN104498472A is main
Have：
1. it is directed in course of reaction and produces substantial amounts of heat, carrying out reaction at normal temperatures can cause to the production safety of system
Impact, therefore should be taken into account the gathering how avoiding heat in the industrial production；Disposably add according to reactant liquor simultaneously, by
Accelerate in reaction rate, will also result in reaction excessively acutely, this also can work the mischief to industrial safety in production；
If 2. adding magnetic material before adding aqueous slkali neutralization to carry out inclusion, because the viscosity of system is little, acid height,
Magnetic material is susceptible to agglomeration, and adds magnetic material after adding aqueous slkali neutralization and carry out inclusion, due to system
Viscosity excessive, magnetic material is difficult to disperse, and these situations easily cause magnetic material and can not be effectively realized uniform inclusion；
3., in gel process, according to neutrallty condition DIRECT GEL, product can be caused to cover in a kettle. and to be difficult to take
Go out, simultaneously because gel time is too fast will also result in the uneven problem of gel；
The immobilized enzyme produced easily causes the problem of enzyme inactivation, therefore it is also contemplated that to how keeping immobilized enzyme
Stability.
Content of the invention
It is an object of the invention to solution the problems of the prior art, a kind of production safety, operability height are provided, produce
Simple process, magnetic inclusion are uniform, the stability of enzyme is high, the production method of the uniform magnetic immobilized enzyme of gel.
For reaching above-mentioned purpose, the present invention adopts the following technical scheme that：
A kind of production method of magnetic immobilized enzyme, comprises the steps：Step 1：Weigh following component by mass parts：Instead
Answer 100 parts of substrate, template solution 50-200 part, ferroferric oxide powder 1-2 part, cellulase 2-30 part；Described reaction bottom
Thing includes tetraethyl orthosilicate or positive quanmethyl silicate；Described template solution be mass percent 28-32% glucose,
Maltose, Fructose, dibenzoyl-L-tartaric, cyclodextrin, carbamide, glycerol, soluble starch, citric acid, ascorbic acid or
The solution of any one in oligopeptide；Step 2：By the amount of substance of the reaction substrate weighing in step 1, and weigh by following mol ratio
Hydrochloric acid, reaction substrate is 100: 1-200: 1 with the mol ratio of hydrochloric acid, and described concentration of hydrochloric acid is equal to or less than 0.2mol/L；Step
Rapid 3：The reaction substrate weighing in step 1 is added in reactor；Step 4：Reaction substrate is cooled to less than 15 DEG C；Step 5：
The hydrochloric acid that weighs in the reaction substrate Deca step 2 in reactor simultaneously stirs until the solution obtaining becomes clear, control
Reaction temperature processed is less than or equal to 30 DEG C；Step 6：In the solution that the template solution weighing in step 1 addition step 5 is obtained,
And stir to mix homogeneously；Step 7：Vacuum distillation removes coproduct ethanol or methanol in reaction solution, obtains colloidal sol；Described
Vacuum distillation pressure be below 0.005MPa, temperature be less than 30 DEG C；Step 8：The colloidal sol Deca concentration obtaining to step 7 is
The sodium hydroxide solution of 0.38-0.42mol/L simultaneously stirs, and drop rate is less than or equal to 1L/min, until pH value is more than or waits
In 3.5, after adding the ferroferric oxide powder weighing in step 1 and cellulase, continue Deca sodium hydroxide solution, until pH
Value meets or exceeds 4.2 end；Step 9：The product that step 8 is obtained is transferred in open containers, is cooled to less than 0 DEG C
And keep more than 10 hours, obtain gel.
Further, reaction substrate described in step 1 also includes dimethyldisiloxane or methyl trisiloxanes, institute
The dimethyldisiloxane stated or the addition of methyl trisiloxanes are less than or equal to the 20% of reaction substrate quality.
Further, pass through psychrolusia in step 4 or setting cooling sandwith layer, coil pipe are realized being cooled to reaction substrate
Less than 15 DEG C.
Further, in step 4 reaction substrate is cooled to less than 10 DEG C.
Further, the preferred 0.10-0.15mol/L of concentration of hydrochloric acid described in step 2.
Further, the speed of the stirring described in step 5, step 6 and step 8 is 60-180 rev/min.
Further, also include step 10 after step 9：Described gel is ground into granularity using pulverizer is
0.2mm particles below, and be dried, using the dried magnetic immobilized enzyme of packaging of aluminium foil bag.
Technical solutions according to the invention with respect to prior art, the beneficial effect of acquirement are：
1st, in step 4 by reaction substrate temperature control below 15 DEG C, it is to avoid under room temperature reaction lead to heat assemble cause
Production dangerous it is ensured that industrial operability.
2nd, add hydrochloric acid in step 5 by the way of Deca, it is to avoid disposable addition leads to reaction excessively violent, in order to
Better control over reaction rate, improve reaction safety.
3rd, in step 7, byproduct of reaction ethanol or methanol are removed by vacuum distillation, it is to avoid ethanol or methanol are to fiber
The vigor of plain enzyme impacts, and follow-up gel time also can be avoided excessively slow simultaneously.
4th, in step 8 by way of adding ferroso-ferric oxide and cellulase in the middle of the N-process, it is to avoid anti-
Early stage is answered to add, because viscosity is little, acid height, ferroso-ferric oxide and cellulase are susceptible to asking of reunion when being directly added into
Topic, has also avoided the phase after the reaction to add simultaneously, and because system viscosity is high, ferroso-ferric oxide and cellulase are difficult scattered
Problem, thus fundamentally solve cladding problem of non-uniform.
5th, using carrying out gel under acid condition in step 9, it is to avoid under neutrallty condition, gel is difficult the problem shifting, and
Avoid the uneven problem of the gel that gel under neutrallty condition occurs.
6th, pass through to add dimethyldisiloxane in reaction substrate or methyl trisiloxanes adjust the feature of final products,
Make magnetic immobilized enzyme have more preferable toughness, it is to avoid broken in use thus lead to reaction vigor decline ask
Topic.
7th, realize cooling condition by the way of using psychrolusia or by cooling sandwith layer or coil pipe, technology is simple and reliable, stable
Property is good.
8th, the magnetic immobilized enzyme adopting packaging of aluminium foil bag to produce, can avoid enzyme to inactivate, thus improving magnetic immobilized enzyme
Storage stability.
9th, because the key step of the present invention is all realized in a kettle., therefore this production method has to equipment requirements not
The advantages such as height, simple process, operability height.
Brief description
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes the part of the present invention, this
Bright schematic description and description is used for explaining the present invention, does not constitute inappropriate limitation of the present invention.In the accompanying drawings：
The equipment schematic diagram that Fig. 1 is used by the embodiment of the present invention；
Specific embodiment
In order that the technical problem to be solved, technical scheme and beneficial effect are clearer, clear, below tie
Close drawings and Examples, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used
To explain the present invention, it is not intended to limit the present invention.
Embodiments of the invention adopt the equipment shown in accompanying drawing 1, and magnetic immobilized enzyme is obtained as follows：
Step 1：Weigh following component by mass parts：100 parts of reaction substrate, 100 parts of template solution, ferroferric oxide powder
2 parts, 20 parts of cellulase；Reaction substrate is tetraethyl orthosilicate and dimethyldisiloxane, wherein tetraethyl orthosilicate quality
Accounting 80%, dimethyldisiloxane quality accounting 20%；Template solution is glucose solution, and glucose quality percentage ratio is
30%；
Step 2：By the amount of substance of the reaction substrate weighing in step 1, and weigh hydrochloric acid, reaction substrate by following mol ratio
Mol ratio with hydrochloric acid is 150: 1, and described concentration of hydrochloric acid is 0.1mol/L；
Step 3：The reaction substrate weighing in step 1 is placed in storage tank 1, and is added in reactor 5 by pump 11；
Step 4：By psychrolusia, reaction substrate is cooled to 8 DEG C；
Step 5：Hydrochloric acid in step 2 is placed in storage tank 2, and by pump 21 to the reaction substrate Deca in reactor 5
Hydrochloric acid simultaneously stirs until the solution obtaining becomes clear, 30 DEG C of controlling reaction temperature, and mixing speed is 100 revs/min；
Step 6：The template solution weighing in step 1 is placed in storage tank 3, and the solution being obtained to step 5 by pump 31
Add template solution, and stir to mix homogeneously, mixing speed is 100 revs/min；
Step 7：Vacuum distillation is carried out up to not by vacuum pump 6 and cooler 7 to step 6 resulting solution in reactor 5
Produce bubble again, to remove the coproduct ethanol in reaction solution, obtain colloidal sol；Described vacuum distillation pressure is
0.005MPa, temperature is 25 DEG C, and coproduct ethanol is cooled back in recycling can 8
Step 8：Close down vacuum pump 6 and cooler 7, the sodium hydroxide solution for 0.4mol/L for the concentration added in storage tank 4,
The above-mentioned sodium hydroxide solution of colloidal sol Deca that obtained to step 7 by pump 41 is simultaneously stirred, and drop rate is 1L/min, and supervises in real time
Survey pH value, until pH value reaches 3.5, by charge door 51 to reactor 5 add the ferroferric oxide powder weighing in step 1 and
Cellulase, continues Deca sodium hydroxide solution, until pH value reaches 4.2, mixing speed is 100 revs/min；
Step 9：The product that step 8 is obtained is transferred to open containers 9 from reactor 5, is cooled to 0 DEG C and keeps
12 hours, obtain gel；
Step 10：It is 0.2mm particles below that described gel is ground into granularity using pulverizer, and is dried,
Using the dried magnetic immobilized enzyme of packaging of aluminium foil bag.
In above-described embodiment, in step 1, final products are adjusted by adding dimethyldisiloxane in reaction substrate
Feature, make magnetic immobilized enzyme have more preferable toughness, it is to avoid breakable problem in use.Lead in step 4
Reaction substrate temperature control at 8 DEG C, be effectively prevent reaction under room temperature and leads to heat to assemble the production danger causing by supercool water-bath
Dangerous it is ensured that industrial operability.In steps of 5, hydrochloric acid is added by Deca mode, it is to avoid disposable addition leads to anti-
Should be excessively violent, in order to better control over reaction rate, improve reaction safety.Removed by vacuum distillation in step 7
Byproduct of reaction ethanol, it is to avoid ethanol impacts to the vigor of cellulase, also can avoid follow-up gel time simultaneously
Cross slow.In step 8 by way of adding ferroso-ferric oxide and cellulase in the middle of the N-process, it is to avoid before the reaction
Phase adds, and because viscosity is little, acid height, ferroso-ferric oxide and cellulase are susceptible to the problem reunited when being directly added into, with
When also avoided the phase after the reaction to add, because system viscosity is high, ferroso-ferric oxide and cellulase are difficult scattered problem,
Thus fundamentally solving cladding problem of non-uniform.In step 9 using carrying out gel under acid condition, it is to avoid neutrallty condition
Lower gel is difficult the problem shifting, and avoids the uneven problem of the gel that gel under neutrallty condition occurs.In step 10,
The magnetic immobilized enzyme being produced using packaging of aluminium foil bag, can avoid enzyme to inactivate, thus improving the stable storage of magnetic immobilized enzyme
Property.Because embodiments of the invention key step is all realized in a kettle., therefore this production method has to equipment requirements not
The advantages such as height, simple process, operability height.
Description above describe the preferred embodiments of the present invention, it is to be understood that the present invention is not limited to above-mentioned enforcement
Example, and should not regard the exclusion to other embodiment as.By the enlightenment of the present invention, those skilled in the art combine known or existing
The change that technology, knowledge are carried out also should be regarded as within the scope of the present invention.
Claims (7)
1. a kind of production method of magnetic immobilized enzyme, is characterized in that：Comprise the steps：
Step 1：Weigh following component by mass parts：100 parts of reaction substrate, template solution 50-200 part, ferroferric oxide powder
1-2 part, cellulase 2-30 part；Described reaction substrate includes tetraethyl orthosilicate or positive quanmethyl silicate；Described template
Solution is mass percent in the glucose of 28-32%, maltose, Fructose, dibenzoyl-L-tartaric, cyclodextrin, urine
The solution of any one in element, glycerol, soluble starch, citric acid, ascorbic acid or oligopeptide；
Step 2：By the amount of substance of the reaction substrate weighing in step 1, and weigh hydrochloric acid, reaction substrate and salt by following mol ratio
The mol ratio of acid is 100: 1-200: 1, and described concentration of hydrochloric acid is equal to or less than 0.2mol/L；
Step 3：The reaction substrate weighing in step 1 is added in reactor；
Step 4：Reaction substrate is cooled to less than 15 DEG C；
Step 5：The hydrochloric acid that weighs in the reaction substrate Deca step 2 in reactor simultaneously stirs until the solution obtaining becomes clear
Clear bright, controlling reaction temperature is less than or equal to 30 DEG C；
Step 6：In the solution that the template solution weighing in step 1 addition step 5 is obtained, and stir to mix homogeneously；
Step 7：Vacuum distillation removes coproduct ethanol or methanol in reaction solution, obtains colloidal sol；Described vacuum distillation pressure
Power is below 0.005MPa, and temperature is less than 30 DEG C；
Step 8：The colloidal sol Deca concentration obtaining to step 7 is the sodium hydroxide solution of 0.38-0.42mol/L and stirs, Deca
Speed is less than or equal to 1L/min, until pH value is more than or equal to 3.5, add in step 1 ferroferric oxide powder that weighs and
After cellulase, continue Deca sodium hydroxide solution, until pH value is more than or equal to 4.2；
Step 9：The product that step 8 is obtained is transferred in open containers, be cooled to less than 0 DEG C and keep 10 hours with
On, obtain gel.
2. a kind of production method of magnetic immobilized enzyme as claimed in claim 1, is characterized in that：Described anti-in step 1
Substrate is answered also to include dimethyldisiloxane or methyl trisiloxanes, described dimethyldisiloxane or methyl trisiloxanes
Addition is less than or equal to the 20% of reaction substrate quality.
3. a kind of production method of magnetic immobilized enzyme as claimed in claim 1, is characterized in that：Pass through cold water in step 4
Bath or setting cooling sandwith layer, coil pipe are to realize for reaction substrate being cooled to less than 15 DEG C.
4. a kind of production method of magnetic immobilized enzyme as claimed in claim 1, is characterized in that：Bottom will be reacted in step 4
Thing is cooled to less than 10 DEG C.
5. a kind of production method of magnetic immobilized enzyme as claimed in claim 1, is characterized in that：Described salt in step 2
The preferred 0.10-0.15mol/L of acid concentration.
6. a kind of production method of magnetic immobilized enzyme as claimed in claim 1, is characterized in that：In step 5, step 6 and step
The speed of the stirring described in rapid 8 is 60-180 rev/min.
7. a kind of production method of the magnetic immobilized enzyme as any one of claim 1 to 6, is characterized in that：In step 9
Also include step 10 afterwards：It is 0.2mm particles below that described gel is ground into granularity using pulverizer, and is done
Dry, using the dried magnetic immobilized enzyme of packaging of aluminium foil bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611135592.3A CN106480000A (en) | 2016-12-09 | 2016-12-09 | A kind of production method of magnetic immobilized enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611135592.3A CN106480000A (en) | 2016-12-09 | 2016-12-09 | A kind of production method of magnetic immobilized enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106480000A true CN106480000A (en) | 2017-03-08 |
Family
ID=58275243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611135592.3A Pending CN106480000A (en) | 2016-12-09 | 2016-12-09 | A kind of production method of magnetic immobilized enzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106480000A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110951719A (en) * | 2019-12-18 | 2020-04-03 | 武汉理工大学 | Biological targeted antibacterial DspB immobilized enzyme and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1986786A (en) * | 2006-12-20 | 2007-06-27 | 东华大学 | Process of preparing fabric fixed papain |
| CN104498472A (en) * | 2014-11-06 | 2015-04-08 | 清华大学 | Magnetic immobilized cellobiase nano mesoporous material and preparation method thereof |
-
2016
- 2016-12-09 CN CN201611135592.3A patent/CN106480000A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1986786A (en) * | 2006-12-20 | 2007-06-27 | 东华大学 | Process of preparing fabric fixed papain |
| CN104498472A (en) * | 2014-11-06 | 2015-04-08 | 清华大学 | Magnetic immobilized cellobiase nano mesoporous material and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张翔 等: "非表面活性剂模板溶胶-凝胶法制备磁性介孔二氧化硅实现纤维二糖酶的原位固定", 《高等学校化学学报》 * |
| 栾明明 等: "溶胶凝胶膜包埋的胰蛋白酶用于高通量蛋白质肽谱分析", 《分析化学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110951719A (en) * | 2019-12-18 | 2020-04-03 | 武汉理工大学 | Biological targeted antibacterial DspB immobilized enzyme and preparation method and application thereof |
| CN110951719B (en) * | 2019-12-18 | 2021-07-20 | 武汉理工大学 | Biological targeted antibacterial DspB immobilized enzyme and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110451525A (en) | A method of quickly preparing the Prussian blue similar object of monoclinic structure | |
| CN103449496A (en) | Nano cerium oxide and preparation method thereof | |
| CN102061112A (en) | Preparation method of composite metal organic framework material colloidal solution and application thereof in optical coatings | |
| CN105140475A (en) | Preparation method of anode material Fe3O4/MoS2 for lithium ion battery | |
| CN102101674A (en) | Method for preparing silica sol | |
| CN105129861B (en) | Preparation method for bismuth ferrite BiFeO3 nanosheet | |
| CN103880080A (en) | Method for preparing vanadium dioxide powder through hydrothermal auxiliary homogeneous precipitation method | |
| CN102702518A (en) | Method for preparing composite material of stannic oxide/polyaniline | |
| CN102627312A (en) | Preparation method of zinc oxide microsphere with nanoflower-shaped microstructure | |
| CN105000567B (en) | A kind of preparation method of high-dispersion nano silicon dioxide | |
| CN103421368A (en) | Preparation method of high-modulus potassium silicate inorganic nano-resin | |
| CN105977486A (en) | Preparation method and application of sodium-rich transition metal silicate as sodium ion battery cathode material | |
| CN105680045B (en) | A kind of preparation method of high stability amorphous manganous silicate | |
| CN108579724A (en) | [010] direction pucherite Nanotube crystal array grown in a kind of electrically conducting transparent substrate and its preparation and application | |
| CN105582696B (en) | A kind of solid defoaming agent and preparation method thereof | |
| CN106480000A (en) | A kind of production method of magnetic immobilized enzyme | |
| CN103183478A (en) | Silicon nitride crucible coating and preparation method thereof | |
| CN105967197B (en) | A kind of water glass solution containing graphene and preparation method thereof | |
| CN104525064A (en) | Alkaline silica sol and preparation method thereof | |
| CN106480130B (en) | A kind of method that magnetic Nano immobilized enzyme hydrolyzing straw can be recycled in application | |
| CN103922402B (en) | Method for preparing NH4V3O8 nanoribbon | |
| CN103265699B (en) | A kind of nano-stannic oxide modifies the preparation method of polyaniline nanotube | |
| CN101483104B (en) | Copolymer solid electrolyte for dye sensitized cell and production method thereof | |
| CN106145691A (en) | A kind of method of the smithsonite zinc silicate of direct growth on a glass substrate | |
| CN105489777B (en) | A kind of calcium titanium photovoltaic material of microcapsule structure and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wei Yan Inventor after: Sun Yafei Inventor after: Yu Weina Inventor after: Ye Peirong Inventor before: Wei Yan Inventor before: Sun Yafei Inventor before: Yu Weina Inventor before: Ye Peirong Inventor before: Tong Longhuan |