CN106479931A - A kind of improved formulations of blood agar culture-medium - Google Patents
A kind of improved formulations of blood agar culture-medium Download PDFInfo
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- CN106479931A CN106479931A CN201610964116.6A CN201610964116A CN106479931A CN 106479931 A CN106479931 A CN 106479931A CN 201610964116 A CN201610964116 A CN 201610964116A CN 106479931 A CN106479931 A CN 106479931A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of improved formulations of blood agar culture-medium, the improved formulations of its blood agar culture-medium by tryptone, beef extract powder, liver digest, yeast extract, starch, sodium chloride, take off fiber sheep blood and agar constitutes, wherein：In per 1000ml culture medium, containing 10～20g of tryptone, 3～8g of beef extract powder, liver 1～4g of digest, 0.5～2g of starch, 3～8g of dusty yeast, 3～8g of sodium chloride, fiber 40～60ml of Sheep Blood, agar 10～20g, pH=7.2 ± 0.2 is taken off.The blood agar culture-medium improved formulations of the present invention can provide full nutrition, maintain bacterial penetration pressure, repair impaired bacterium.Formula after being optimized using the present invention, not only can meet comprehensive needs of common bacteria and most of severe bacteria, and be conducive to culture and the identification of bacterium.
Description
Technical field
The invention belongs to technical field of bioengineering, is related to a kind of improved formulations of blood agar basal medium, specifically relates to
And a kind of improved formulations that can meet common bacteria and the blood agar basal medium of the comprehensive needs of most of severe bacteria.
Background technology
Existing blood agar basal medium is substantially in nutrient agar (by peptone, sodium chloride, beef extract powder, fine jade
Fat constitute) on the basis of add Sheep Blood make.Due to the enforcement of 2015 Chinese Pharmacopoeia of new edition, TSA instead of battalion comprehensively
Foster agar, in nutrient content, nutrient agar is not very general equilibrium, and the repair ability for impaired bacterium is poor, existing
Less it is suitable in modern further strict detection.In the same manner, the blood agar basal medium that sets up on the basis of nutrient agar
It is accomplished by being improved.
Content of the invention
It is an object of the invention to provide a kind of can provide full nutrition, maintain bacterial penetration pressure, repair impaired bacterium
The improved formulations of blood agar culture-medium.
The purpose of the present invention is achieved through the following technical solutions：
A kind of improved formulations of blood agar culture-medium, by tryptone, beef extract powder, liver digest, yeast extract,
Starch, sodium chloride, de- fiber sheep blood and agar composition, wherein：In per 1000ml culture medium, containing 10～20g of tryptone, ox
Meat leaching 3～8g of powder, liver 1～4g of digest, 0.5～2g of starch, 3～8g of dusty yeast, 3～8g of sodium chloride, de- fiber Sheep Blood
40～60ml, agar 10～20g, pH=7.2 ± 0.2.
In the present invention, the tryptone is domestic, makes (this as raw material with fresh beef and ox bone after pancreatin digestion
In can not select import tryptone, import tryptone be casein digests, lack sulfur-containing amino acid).Egg can be compared
White peptone provides more carbon nitrogen sources, vitamin and growth factor.
In the present invention, the beef extract powder is domestic, with fresh beef as raw material, thermally treated, filtration, concentration, drying
Beef original powder is made, then beef original powder is obtained through operations such as hydrolysis, separation of solid and liquid, refrigeration, filtration, concentration and dryings.Miscellaneous
Matter is few, using the teaching of the invention it is possible to provide organic acid, nucleotides, mineral matter, vitamin etc..
In the present invention, the liver digest is import, liver enzymolysis product, using the teaching of the invention it is possible to provide abundant hepatic glycogen, dimension are given birth to
Various bacteriums are had obvious growth promoting function by element and amino acid, particularly fabulous with Amoeba effect to brucella,
It additionally is able to preferably cultivate anaerobic bacteria.
In the present invention, the starch is domestic.Starch can provide part carbon and nitrogen, and promote growth and the increasing of Neisseria
Streptococcic haematolysis property by force, and some toxin bacteriogenic can be absorbed, bacterial growth can be made better.
In the present invention, the yeast extract is import, with fresh yeast as raw material, using the teaching of the invention it is possible to provide B family vitamin and various
Amino acid and carbohydrate (predominantly glycogen and trehalose).
In the present invention, the sodium chloride is domestic, is able to maintain that osmotic pressure.
In the present invention, the de- fiber sheep blood is self-control, and selected sheep health, non-injection of antibiotics take off fiber after blood drawing
It is obtained, is the good nutrition material of bacterial growth breeding.
The blood agar culture-medium improved formulations of the present invention can provide full nutrition, maintain bacterial penetration pressure, repair impaired
Bacterium.Formula after being optimized using the present invention, not only can meet comprehensive needs of common bacteria and most of severe bacteria, and
Be conducive to culture and the identification of bacterium.
Specific embodiment
Technical scheme is further described with reference to embodiment, but is not limited thereto, every right
Technical solution of the present invention is modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention, all should contain
Cover in protection scope of the present invention.
Embodiment 1：
The improved formulations of the blood agar culture-medium that the present embodiment is provided are by tryptone, beef extract powder, liver digest, ferment
Female extract, starch, sodium chloride, de- fiber sheep blood and agar composition, wherein：In per 1000ml culture medium, containing tryptone
15g, beef extract powder 5g, liver digest 2.5g, starch 1g, dusty yeast 5g, sodium chloride 5g, de- fiber Sheep Blood 50ml, agar
15g, pH=7.2 ± 0.2.
The preparation process of above-mentioned culture medium is as follows：By tryptone, beef extract powder, liver digest, starch, yeast leaching
Powder, sodium chloride, agar proportionally mix, and add purified water constant volume heating for dissolving and adjust pH value to 7.2 ± 0.2, through 121 DEG C
Sterilizing 15 minutes, is cooled to 50 DEG C, is proportionally added into aseptic de-fiber sheep blood, irrigates flat board, cultivated after condensation after mixing
Base finished product, standby.
The new blood agar basal medium that is made using mentioned component carries out bacterium with classical blood agar basal medium
Culture experiment is contrasted, as a result as shown in table 1.
Bacterial strain uses therefor：Neisseria meningitidis (CMCC 29001), streptococcus pneumonia (ATCC6305), hemolytic chain
Coccus (CMCC 32210), streptococcus fecalis (ATCC 29212).
Every kind of bacterium is all coated culture with same concentration bacterium solution, and does 3 groups of parallel laboratory tests.
As shown in Table 1, from experimental result, bacterium detection number is all better than classics with state to the culture medium prescription of the present embodiment
Formula.
Table 1
Embodiment 2：
The present embodiment is as different from Example 1：In per 1000ml culture medium, containing tryptone 12g, beef extract powder
4g, liver digest 3g, starch 1.5g, dusty yeast 6g, sodium chloride 6g, take off fiber Sheep Blood 45ml, agar 12g, pH=7.2 ±
0.2.
Embodiment 3：
The present embodiment is as different from Example 1：In per 1000ml culture medium, containing tryptone 18g, beef extract powder
6g, liver digest 1.5g, starch 0.5g, dusty yeast 4g, sodium chloride 4g, de- fiber Sheep Blood 55ml, agar 18g, pH=7.2
±0.2.
Claims (4)
1. a kind of improved formulations of blood agar culture-medium, it is characterised in that the improved formulations of the blood agar culture-medium are by tryptose
Peptone, beef extract powder, liver digest, yeast extract, starch, sodium chloride, de- fiber sheep blood and agar composition, wherein：Per
In 1000ml culture medium, containing 10～20g of tryptone, 3～8g of beef extract powder, liver 1～4g of digest, 0.5～2g of starch,
3～8g of dusty yeast, 3～8g of sodium chloride, de- fiber 40～60ml of Sheep Blood, agar 10～20g, pH=7.2 ± 0.2.
2. improved formulations of blood agar culture-medium according to claim 1, it is characterised in that in per 1000ml culture medium, contain
There are tryptone 15g, beef extract powder 5g, liver digest 2.5g, starch 1g, dusty yeast 5g, sodium chloride 5g, take off fiber Sheep Blood
50ml, agar 15g, pH=7.2 ± 0.2.
3. improved formulations of blood agar culture-medium according to claim 1, it is characterised in that in per 1000ml culture medium, contain
There are tryptone 12g, beef extract powder 4g, liver digest 3g, starch 1.5g, dusty yeast 6g, sodium chloride 6g, take off fiber Sheep Blood
45ml, agar 12g, pH=7.2 ± 0.2.
4. improved formulations of blood agar culture-medium according to claim 1, it is characterised in that in per 1000ml culture medium, contain
There are tryptone 18g, beef extract powder 6g, liver digest 1.5g, starch 0.5g, dusty yeast 4g, sodium chloride 4g, take off fiber sheep
Blood 55ml, agar 18g, pH=7.2 ± 0.2.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107400642A (en) * | 2017-07-24 | 2017-11-28 | 内蒙古东达獭兔循环产业研究院 | A kind of bacteriotrophy culture medium and preparation method thereof |
| CN110747144A (en) * | 2019-11-27 | 2020-02-04 | 中秀科技股份有限公司 | Gonococcus agar plate and preparation method thereof |
| CN110819570A (en) * | 2019-11-27 | 2020-02-21 | 中秀科技股份有限公司 | Blood agar plate and preparation method thereof |
| CN111518733A (en) * | 2020-05-27 | 2020-08-11 | 深圳市崇源科技服务中心 | Columbia blood agar culture medium and preparation method and application thereof |
| WO2022146242A1 (en) * | 2020-12-29 | 2022-07-07 | Nanobi̇otech Arge İnovasyon Sağlik Ürünleri̇ Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇ | Composition of a nanoparticle charged bioadsorbent blood culture and its production method |
| CN117778245A (en) * | 2023-12-21 | 2024-03-29 | 琼海市人民医院 | Streptomyces candidus culture medium |
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| CN117778245A (en) * | 2023-12-21 | 2024-03-29 | 琼海市人民医院 | Streptomyces candidus culture medium |
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Application publication date: 20170308 |