CN106479894A - A kind of preparation method of the microbe granular for producing biodiesel - Google Patents
A kind of preparation method of the microbe granular for producing biodiesel Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of the microbe granular for producing biodiesel, belong to technical field of biodiesel preparation.The present invention is by fermenting to the microorganism in Pond Silt and waste oil, change the microorganism structure in Pond Silt, subsequently pass through to extract its interior microorganism, carry out screening and culturing, induce mutation using β roentgenization, drip washing media surface collect leacheate after culture terminates, collect the strain after screening, subsequently by scolecite, hydrochloric acid solution and lauryl mercaptan mixing carry out surface modification, activated with high temperature, soaked using glucose solution and obtain nutrient, take out filtering residue and leacheate, sodium alginate mixed freezing is dried, strain is made to be carried on filtering residue, and wrapped up, finally pulverize and sieve and can must be used for producing the microbe granular of biodiesel.The biodiesel environmental protection of present invention preparation, will not can significantly improve reaction conversion ratio to environment.
Description
Technical field
The invention discloses a kind of preparation method of the microbe granular for producing biodiesel, belong to biodiesel system
Standby technical field.
Background technology
Biodiesel is with animal and plant fat as raw material, is made through Ester exchange under catalyst action with methanol or ethanol
Diesel oil.Generally oil-yielding shrubs fruit, engineering microalgae, the biodiesel such as the oil crop such as Semen sojae atricolor and Semen Brassicae campestriss, Elaeis guineensis Jacq. and Chinese pistache
Deng the reproducibility diesel fuel of the replaced petrifaction diesel made by ester exchange process for raw oil, it is the renewable energy of cleaning
Source, is the petroleum diesel succedaneum of high-quality.Biodiesel is one kind of biomass energy, and it is the technology such as biomass economy thermal cracking
A kind of mono alkyl ester of the long-chain fatty acid obtaining.
Biodiesel is the mixture of the high complicated organic principle of oxygen content, and these mixture are mainly number molecular weight
Big Organic substance, almost includes the oxygen-bearing organic matter of all kinds, such as:Ether, ester, aldehyde, ketone, phenol, organic acid, alcohol etc..Biological bavin
Oil is typical " green energy resource ", greatly develops biodiesel to sustainable economic development, advances energy substitution, mitigates environment pressure
Power, controls urban atmospheric pollution to have important strategic importance.
The manufacturing of biodiesel at present, generally adopts the production technology of chemical method, Physical and biological enzyme,
Concentrated acid or concentrated base is generally adopted as catalyst in esterification process, larger to the damage ratio of environment, and exist when producing in ester friendship
Change that oils and fatss in reaction are immiscible with methanol, the problem that catalyst can not fully contact with reactant.
Content of the invention
Present invention mainly solves technical problem:For presence oils and fatss and first in ester exchange reaction during current diesel production
Alcohol is immiscible, and catalyst can not fully contact with reactant, and in esterification process generally using concentrated acid or concentrated base as urging
Agent, the problem larger to the damage ratio of environment, provide a kind of by carrying out sending out to the microorganism in Pond Silt and waste oil
Ferment, changes the microorganism structure in Pond Silt, subsequently passes through to extract its interior microorganism, carries out screening and culturing, using β ray
Radiation-induced mutation, drip washing media surface collect leacheate after culture terminates, collect the strain after screening, subsequently by calcium
Zeolite, hydrochloric acid solution and lauryl mercaptan mixing carry out surface modification, are activated with high temperature, are soaked using glucose solution
Obtain nutrient, take out filtering residue and be dried with leacheate, sodium alginate mixed freezing, make strain be carried on filtering residue, and wrapped up,
Finally pulverize and sieve and can must be used for producing the microbe granular of biodiesel, preparation technology of the present invention is easy, using the present invention
The biodiesel environmental protection of microbe granular preparation, will not be to environment during use, and when producing, catalyst can fill
Tap touches reactant, can significantly improve reaction conversion ratio, improves production capacity and product quality, has larger application space.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1)In mass ratio 3:1, take water content to be that 40~45% Pond Silt are put in fermentation vat with waste oil, in fermentation pool surface
Mulching straw, cladding thickness is 5~8cm, sets fermentation vat temperature as 32~40 DEG C, ferments 3~5 days, during the fermentation, often
9~11h, adds waste oil again into fermentation vat, and addition is to add the 20~40% of trench oil volume for the first time;
(2)After above-mentioned fermentation ends, the mixture in fermentation vat is filtered, collect filtering residue, by filtering residue natural drying extremely
Water content is 23~26%, subsequently by the filtering residue after dry in the sun and 1.8mol/L glucose solution in mass ratio 1:3 put into blender
In, 40~50min is stirred with 190r/min, then stands 1~2h, collect the supernatant, obtain mixed bacteria liquid;
(3)Count by weight, take 40~45 portions of sucrose, 32~36 parts of yeast extracts, 18~21 parts of agar, 4~6 parts of carbamide, 2
~3 parts of Magnesium sulfate heptahydrate, 1~2 part of potassium dihydrogen phosphate, 0.5~0.8 part of dipotassium hydrogen phosphate and 0.4~0.7 part of sulfinpyrazone, stirring
Uniformly, put into sterilizing in high-pressure sterilizing pot, obtain culture medium, by inoculum concentration 13~16%, above-mentioned mixed bacteria liquid is seeded to training
Foster base;
(4)Above-mentioned postvaccinal culture medium is moved in shaken cultivation case, design temperature is 30~40 DEG C, cultivate 18 with 130rpm
~22h, every 3~5h in incubation, using β roentgenization culture medium 3~5s, after culture terminates, using sterilized water with
3mL/s drip washing media surface, until its no mycelia, collects leacheate;
(5)In mass ratio 4:6:1, take scolecite, mass fraction to be that 10% hydrochloric acid solution and lauryl mercaptan mix all successively
Even, stand 30~40min, then filtered, filtering residue is put in Muffle furnace, design temperature is 400~450 DEG C, dry 1~
2h, dried filtering residue is taken out, and is soaked in 2.0mol/L glucose solution and takes out, by weight after standing 30~40min
Number meter, take 40~50 parts of above-mentioned leacheates, 28~31 parts soak after filtering residue and 8~11 parts of sodium alginates, put in container, with
120r/min stirs 2~3h, subsequently carries out lyophilization, dried object is put in pulverizer and pulverizes, and crosses 200 mesh sieves, collected
Sieve granule, you can must be used for producing the microbe granular of biodiesel.
The application process of the present invention is:By weight 100:30~35:1, the microbe granular that be obtained the present invention and first
Alcohol, concentrated sulphuric acid hybrid reaction simultaneously prepare the prepared biodiesel of biodiesel process by routine, and controlling reaction temperature is 40~50
℃.After testing, the microbe granular environmental protection that the present invention is obtained, prepare the response time in biodiesel process shorten 50~
60%, biodiesel yield reaches 50~65%, and the biodiesel high purity more than 99.6% being obtained.
The invention has the beneficial effects as follows:
(1)The biodiesel yield made using microbe granular of the present invention is higher, and the preparation process response time is shorter, enables
The target that biodiesel efficiently produces;
(2)It is raw material that the present invention adopts Pond Silt and waste oil, prepares the microbe granular for producing biodiesel, preparation
Simple process, the biodiesel environmental protection made, high purity more than 99.6%.
Specific embodiment
First in mass ratio 3:1, take water content to be that 40~45% Pond Silt are put in fermentation vat with waste oil, in fermentation
Pool surface mulching straw, cladding thickness is 5~8cm, sets fermentation vat temperature as 32~40 DEG C, ferments 3~5 days, was fermenting
Cheng Zhong, every 9~11h, add waste oil again into fermentation vat, and addition is to add the 20~40% of trench oil volume for the first time;With
Afterwards after above-mentioned fermentation ends, the mixture in fermentation vat is filtered, collect filtering residue, by filtering residue natural drying to water content
For 23~26%, subsequently by the filtering residue after dry in the sun and 1.8mol/L glucose solution in mass ratio 1:3 put in blender, with
190r/min stirs 40~50min, then stands 1~2h, collects the supernatant, obtains mixed bacteria liquid;Count by weight again, take 40
~45 parts of sucrose, 32~36 parts of yeast extracts, 18~21 parts of agar, 4~6 parts of carbamide, 2~3 parts of Magnesium sulfate heptahydrate, 1~2 part of phosphorus
Acid dihydride potassium, 0.5~0.8 part of dipotassium hydrogen phosphate and 0.4~0.7 part of sulfinpyrazone, stir, and put into sterilization in high-pressure sterilizing pot
Sterilization, obtains culture medium, by inoculum concentration 13~16%, above-mentioned mixed bacteria liquid is seeded to culture medium;Subsequently by above-mentioned postvaccinal training
Foster base moves in shaken cultivation case, and design temperature is 30~40 DEG C, cultivates 18~22h with 130rpm, every 3 in incubation~
5h, using β roentgenization culture medium 3~5s, after culture terminates, using sterilized water with 3mL/s drip washing media surface up to it
No mycelia, collects leacheate;Last in mass ratio 4:6:1, take scolecite, mass fraction to be 10% hydrochloric acid solution and 12 successively
Alkyl hydrosulfide mix homogeneously, stands 30~40min, then is filtered, filtering residue is put in Muffle furnace, and design temperature is 400~
450 DEG C, 1~2h is dried, dried filtering residue is taken out, after being soaked in standing 30~40min in 2.0mol/L glucose solution
Take out, count by weight, take 40~50 parts of above-mentioned leacheates, 28~31 parts soak after filtering residue and 8~11 parts of sodium alginates,
Put in container, 2~3h is stirred with 120r/min, subsequently carries out lyophilization, dried object is put in pulverizer and pulverizes, mistake
200 mesh sieves, collect the granule that sieves, you can must be used for producing the microbe granular of biodiesel.
Example 1
First in mass ratio 3:1, take water content to be that 40% Pond Silt is put in fermentation vat with waste oil, cover in fermentation pool surface
Lid Caulis et Folium Oryzae, cladding thickness is 5cm, sets fermentation vat temperature as 32 DEG C, ferments 3 days, during the fermentation, every 9h, to fermentation vat
Inside add waste oil again, addition is to add the 20% of trench oil volume for the first time;Subsequently after above-mentioned fermentation ends, to fermentation
Mixture in pond is filtered, and collects filtering residue, filtering residue natural drying to water content is 23%, subsequently by the filtering residue after dry in the sun
With 1.8mol/L glucose solution in mass ratio 1:3 put in blender, stir 40min with 190r/min, then stand 1h, collect
The supernatant, obtains mixed bacteria liquid;Count by weight again, take 40 portions of sucrose, 32 parts of yeast extracts, 18 parts of agar, 4 parts of carbamide, 2
Part Magnesium sulfate heptahydrate, 1 part of potassium dihydrogen phosphate, 0.5 part of dipotassium hydrogen phosphate and 0.4 part of sulfinpyrazone, stir, put into autoclaving
Sterilizing in pot, obtains culture medium, by inoculum concentration 13%, above-mentioned mixed bacteria liquid is seeded to culture medium;Subsequently by after above-mentioned inoculation
Culture medium move in shaken cultivation case, design temperature be 30 DEG C, with 130rpm cultivate 18h, every 3h in incubation, use
β roentgenization culture medium 3s, after culture terminates, using sterilized water with 3mL/s drip washing media surface until its no mycelia, collects
Leacheate;Last in mass ratio 4:6:1, take scolecite, mass fraction to be 10% hydrochloric acid solution and lauryl mercaptan mixing successively
Uniformly, stand 30min, then filtered, filtering residue is put in Muffle furnace, design temperature is 400 DEG C, 1h is dried, after being dried
Filtering residue take out, take out after being soaked in 2.0mol/L glucose solution standing 30min, count by weight, take 40 parts above-mentioned
Filtering residue after leacheate, 28 parts of immersions and 8 parts of sodium alginates, put in container, stir 2h with 120r/min, subsequently freezed
It is dried, dried object is put in pulverizer and pulverizes, cross 200 mesh sieves, collect the granule that sieves, you can must be used for producing biodiesel
Microbe granular.
This example operation is easy, during use, by weight 100:30:1, the microbe granular that be obtained the present invention and first
Alcohol, concentrated sulphuric acid hybrid reaction simultaneously prepare the prepared biodiesel of biodiesel process by routine, and controlling reaction temperature is 40 DEG C.Warp
Detection, the microbe granular environmental protection that the present invention is obtained, preparing the response time in biodiesel process shortens 50%, biological
Diesel yield reaches 50%, and the biodiesel high purity 99.7% being obtained.
Example 2
First in mass ratio 3:1, take water content to be that 43% Pond Silt is put in fermentation vat with waste oil, cover in fermentation pool surface
Lid Caulis et Folium Oryzae, cladding thickness is 7cm, sets fermentation vat temperature as 36 DEG C, ferments 4 days, during the fermentation, every 10h, to fermentation vat
Inside add waste oil again, addition is to add the 30% of trench oil volume for the first time;Subsequently after above-mentioned fermentation ends, to fermentation
Mixture in pond is filtered, and collects filtering residue, filtering residue natural drying to water content is 25%, subsequently by the filtering residue after dry in the sun
With 1.8mol/L glucose solution in mass ratio 1:3 put in blender, stir 45min with 190r/min, then stand 1.5h, receive
The collection supernatant, obtains mixed bacteria liquid;Count by weight again, take 43 portions of sucrose, 34 parts of yeast extracts, 20 parts of agar, 5 parts of urine
Element, 2.5 parts of Magnesium sulfate heptahydrate, 1.5 parts of potassium dihydrogen phosphates, 0.7 part of dipotassium hydrogen phosphate and 0.6 part of sulfinpyrazone, stir, put into
Sterilizing in high-pressure sterilizing pot, obtains culture medium, by inoculum concentration 15%, above-mentioned mixed bacteria liquid is seeded to culture medium;Subsequently will be upper
State postvaccinal culture medium and move in shaken cultivation case, design temperature is 35 DEG C, 20h is cultivated with 130rpm, in incubation
Every 4h, using β roentgenization culture medium 4s, after culture terminates, using sterilized water with 3mL/s drip washing media surface up to it no
Mycelia, collects leacheate;Last in mass ratio 4:6:1, take scolecite, mass fraction to be 10% hydrochloric acid solution and dodecane successively
Base mercaptan mix homogeneously, stands 35min, then is filtered, filtering residue is put in Muffle furnace, and design temperature is 425 DEG C, is dried
1.5h, dried filtering residue is taken out, and is soaked in 2.0mol/L glucose solution and takes out, by weight after standing 35min
Meter, take 45 parts of above-mentioned leacheates, 30 parts soak after filtering residue and 10 parts of sodium alginates, put in container, with 120r/min stirring
2.5h, subsequently carries out lyophilization, dried object is put in pulverizer and pulverizes, and crosses 200 mesh sieves, collects the granule that sieves, you can
For producing the microbe granular of biodiesel.
This example operation is easy, during use, by weight 100:33:1, the microbe granular that be obtained the present invention and first
Alcohol, concentrated sulphuric acid hybrid reaction simultaneously prepare the prepared biodiesel of biodiesel process by routine, and controlling reaction temperature is 45 DEG C.Warp
Detection, the microbe granular environmental protection that the present invention is obtained, preparing the response time in biodiesel process shortens 55%, biological
Diesel yield reaches 57%, and the biodiesel high purity 99.8% being obtained.
Example 3
First in mass ratio 3:1, take water content to be that 45% Pond Silt is put in fermentation vat with waste oil, cover in fermentation pool surface
Lid Caulis et Folium Oryzae, cladding thickness is 8cm, sets fermentation vat temperature as 40 DEG C, ferments 5 days, during the fermentation, every 11h, to fermentation vat
Inside add waste oil again, addition is to add the 40% of trench oil volume for the first time;Subsequently after above-mentioned fermentation ends, to fermentation
Mixture in pond is filtered, and collects filtering residue, filtering residue natural drying to water content is 26%, subsequently by the filtering residue after dry in the sun
With 1.8mol/L glucose solution in mass ratio 1:3 put in blender, stir 50min with 190r/min, then stand 2h, collect
The supernatant, obtains mixed bacteria liquid;Count by weight again, take 45 portions of sucrose, 36 parts of yeast extracts, 21 parts of agar, 6 parts of carbamide, 3
Part Magnesium sulfate heptahydrate, 2 parts of potassium dihydrogen phosphates, 0.8 part of dipotassium hydrogen phosphate and 0.7 part of sulfinpyrazone, stir, put into autoclaving
Sterilizing in pot, obtains culture medium, by inoculum concentration 16%, above-mentioned mixed bacteria liquid is seeded to culture medium;Subsequently by after above-mentioned inoculation
Culture medium move in shaken cultivation case, design temperature be 40 DEG C, with 130rpm cultivate 22h, every 5h in incubation, use
β roentgenization culture medium 5s, after culture terminates, using sterilized water with 3mL/s drip washing media surface until its no mycelia, collects
Leacheate;Last in mass ratio 4:6:1, take scolecite, mass fraction to be 10% hydrochloric acid solution and lauryl mercaptan mixing successively
Uniformly, stand 40min, then filtered, filtering residue is put in Muffle furnace, design temperature is 450 DEG C, 2h is dried, after being dried
Filtering residue take out, take out after being soaked in 2.0mol/L glucose solution standing 40min, count by weight, take 50 parts above-mentioned
Filtering residue after leacheate, 31 parts of immersions and 11 parts of sodium alginates, put in container, stir 3h with 120r/min, subsequently carry out cold
Lyophilizing is dry, dried object is put in pulverizer and pulverizes, and crosses 200 mesh sieves, collects the granule that sieves, you can must be used for producing biodiesel
Microbe granular.
This example operation is easy, during use, by weight 100:35:1, the microbe granular that be obtained the present invention and first
Alcohol, concentrated sulphuric acid hybrid reaction simultaneously prepare the prepared biodiesel of biodiesel process by routine, and controlling reaction temperature is 50 DEG C.Warp
Detection, the microbe granular environmental protection that the present invention is obtained, preparing the response time in biodiesel process shortens 60%, biological
Diesel yield reaches 65%, and the biodiesel high purity 99.9% being obtained.
Claims (1)
1. a kind of preparation method of the microbe granular for producing biodiesel is it is characterised in that concrete preparation process is:
(1)In mass ratio 3:1, take water content to be that 40~45% Pond Silt are put in fermentation vat with waste oil, in fermentation pool surface
Mulching straw, cladding thickness is 5~8cm, sets fermentation vat temperature as 32~40 DEG C, ferments 3~5 days, during the fermentation, often
9~11h, adds waste oil again into fermentation vat, and addition is to add the 20~40% of trench oil volume for the first time;
(2)After above-mentioned fermentation ends, the mixture in fermentation vat is filtered, collect filtering residue, by filtering residue natural drying extremely
Water content is 23~26%, subsequently by the filtering residue after dry in the sun and 1.8mol/L glucose solution in mass ratio 1:3 put into blender
In, 40~50min is stirred with 190r/min, then stands 1~2h, collect the supernatant, obtain mixed bacteria liquid;
(3)Count by weight, take 40~45 portions of sucrose, 32~36 parts of yeast extracts, 18~21 parts of agar, 4~6 parts of carbamide, 2
~3 parts of Magnesium sulfate heptahydrate, 1~2 part of potassium dihydrogen phosphate, 0.5~0.8 part of dipotassium hydrogen phosphate and 0.4~0.7 part of sulfinpyrazone, stirring
Uniformly, put into sterilizing in high-pressure sterilizing pot, obtain culture medium, by inoculum concentration 13~16%, above-mentioned mixed bacteria liquid is seeded to training
Foster base;
(4)Above-mentioned postvaccinal culture medium is moved in shaken cultivation case, design temperature is 30~40 DEG C, cultivate 18 with 130rpm
~22h, every 3~5h in incubation, using β roentgenization culture medium 3~5s, after culture terminates, using sterilized water with
3mL/s drip washing media surface, until its no mycelia, collects leacheate;
(5)In mass ratio 4:6:1, take scolecite, mass fraction to be that 10% hydrochloric acid solution and lauryl mercaptan mix all successively
Even, stand 30~40min, then filtered, filtering residue is put in Muffle furnace, design temperature is 400~450 DEG C, dry 1~
2h, dried filtering residue is taken out, and is soaked in 2.0mol/L glucose solution and takes out, by weight after standing 30~40min
Number meter, take 40~50 parts of above-mentioned leacheates, 28~31 parts soak after filtering residue and 8~11 parts of sodium alginates, put in container, with
120r/min stirs 2~3h, subsequently carries out lyophilization, dried object is put in pulverizer and pulverizes, and crosses 200 mesh sieves, collected
Sieve granule, you can must be used for producing the microbe granular of biodiesel.
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Cited By (2)
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CN112457106A (en) * | 2020-12-11 | 2021-03-09 | 成都奉咸棠环保科技有限公司 | Preparation method of compound microbial fertilizer |
CN114716283A (en) * | 2022-04-07 | 2022-07-08 | 广东百林生态科技股份有限公司 | Preparation method of organic fertilizer for ecological restoration |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1803984A (en) * | 2006-01-25 | 2006-07-19 | 任春山 | Process for preparing biological diesel oil by utilizing canal mud |
CN102808000A (en) * | 2012-08-23 | 2012-12-05 | 辽宁科技大学 | Recycling grading utilization and carbon sequestration treatment method for municipal solid waste |
CN102952754A (en) * | 2011-08-30 | 2013-03-06 | 天津聚贤技术研发中心 | Immobilized cell biodiesel |
US20140038273A1 (en) * | 2009-06-30 | 2014-02-06 | Nanobiosym, Inc. | Nano Biofuel Production Processes: Using Nantechnology to Enhance Produciton fo Biofuels |
-
2016
- 2016-10-09 CN CN201610879897.9A patent/CN106479894A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
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