CN106474565A - A kind of tussah silk fibroin is combined the preparation method of maltodextrin active scaffold material - Google Patents
A kind of tussah silk fibroin is combined the preparation method of maltodextrin active scaffold material Download PDFInfo
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- CN106474565A CN106474565A CN201611126018.1A CN201611126018A CN106474565A CN 106474565 A CN106474565 A CN 106474565A CN 201611126018 A CN201611126018 A CN 201611126018A CN 106474565 A CN106474565 A CN 106474565A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/48—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with macromolecular fillers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/426—Immunomodulating agents, i.e. cytokines, interleukins, interferons
Abstract
The present invention provides a kind of tussah silk fibroin to be combined the preparation method of the active scaffold material of maltodextrin, and this compound support frame material is with tussah silk fibroin and maltodextrin as main raw material(s).Tussah silk fibroin can promote fibroblast to adhere to；Maltodextrin has material modification agent, porogen, cytotrophy agent, the effect of neutrophils chemotactic agent；Adjust pore-size distribution and the porosity of material in the preparation technology of this material using the ultrasonic leaching hole forming technology that is separated, be conducive to mechanical property and the degradation property of flexible material, realize scaffold degradation rate and match with cell growth rate；Timbering material preparation process is simple of the present invention, with short production cycle, there is higher clinical value, be particularly suited for the preparation of artificial skin.
Description
Technical field
The present invention relates to tissue engineering bracket material field, it is active that particularly a kind of tussah silk fibroin is combined maltodextrin
The preparation method of timbering material.
Background technology
Organizational project is degradable biomaterial to be made the support in given shape and aperture, in the effect of somatomedin
Under, in conjunction with the living cells in particular organization source, build an implantation body having biological activity, Ran Houzhi after In vitro culture in advance
Enter body, repair or substitute defective tissue or organ, ultimately form corresponding tissue or organ and exercise corresponding engineering.Cause
This, the core of organizational project is living cells and is available for the timbering material that cell carries out vital movement, and mutual between them
Total use.Timbering material is the most basic framework of engineered tissue, is that cell provides acquisition nutrition, gas exchange, excretion
Waste and the place of growth metabolism, are also to form the new tissue with form and function, the material base of organ, and traditional
Two-dimensional structure（As cell culture）It is essentially different.The industrialization of timbering material is the premise bar of organizational project industrialization
Part.
The requirement more and more higher to biomaterial for the organizational project：（1）Biological safety is good, no sensitization nontoxic to human body and
Non-carcinogenesis.（2）Biocompatibility is good, and human body, tissue, Hemic and immune system are had no adverse reaction.（3）Should have good
Cell adhesion ability, can simulate as far as possible suitable cell growth, hypertrophy, differentiation physiological environment.（4）Must biology drop
Solution, catabolite should be nontoxic, can be by organism metabolism or removing, and degradation time should be matched with agglutination and regenerative process, has
It is suitable to the mechanical characteristic operating, the mechanical characteristic after degraded is changed and should be mated with healing or regeneration event.（5）Should have and suitably ooze
Permeability and expected machinability, can have specific 3 dimensional coil geometry by the formation random-shaping of tissue, organ, have certain
Aperture and interconnective three dimensional structure.（6）Support will have certain pliability, organically can combine closely one with body
Rise.
Organization engineering skin is one of therapy for the treatment of skin trauma and defect.Extensively, clinic makes its material source
With respond well.Material selects there is pure natural timbering material, synthesis timbering material, also has the timbering material that both are combined.But
On the whole, more preponderate and leading position remain pure natural timbering material.
Silkworm is divided into silkworm（Bombyxmori Linnaeuss）With Bombyx mandarina Moore (Antherea pernyi Guerin-Meneville, giant silkworm, Semen Ricini silkworm etc.) two classes, only having by industrialized utilization so far
Cocoon and two kinds of tussah cocoon, in the research of most fibroin materials or report, raw materials used is all domestic silkworm silk.
Tussah silk is made up of 18 kinds of aminoacid, but recent studies indicate that：Tussah silk Soviet Union albumen in containing arginine-
Glycine-aspartic acid（RGD）Tripeptide sequence, RGD sequence is combined with extracellular ligand as cellular integration element receptor
Recognition site, the interaction between street cell and extracellular matrix and cell, the adhesion to cell for the support can be promoted,
Adhesive rate and the appreciation rate so that cell is much better than on Bombyx mori Silk Fibroin film in the adhesive rate on tussah silk peptide film and appreciation rate, is regarded
For the new biomedical material that can be used for human body.Special due to containing more cell in the primary structure of tussah silk fibroin
Different in nature adhesion sequence RGD, and in silkworm, do not contain RGD sequence, the therefore life of tussah silk fibroin stroma tissue engineering support material
Thing performance may be more preferable than silk fibroin protein.
The patent documentation of Publication No. CN101274109A discloses a kind of foaming, and to prepare tussah silk fibroin-high polymer many
The method that hole mutually passes grid shaped bracket material, but the method is due to employing synthetic high polymer, thus leading to support in human body
Degradation cycle be difficult to control to and adjust, do not utilize wide clinical application.Synthetic polymer is inert biologically, has bigger
Measurable characteristic, the concordance between batch, in special-purpose, unique advance is so that these implants are generally in patient and trouble
Between person, the diversity ratio enzymolysis property polymeric implant between position and position is less, but there is hydrophobicity and cell adhesion
The defects such as difference, some bio signal chains being blended with biological activity can be to a certain extent to synthetic polymer formation hybrid material
Improve these defects.But the physics of synthesizing polymeric material, chemistry, biology and bio-mechanical property can change over,
The catabolite producing is also variant with the tissue compatible level of raw material, and therefore, the biocompatibility of synthetic polymer also needs
Want the inspection of time.
The patent documentation of Publication No. 100551449A discloses a kind of preparation of tussah silk fibroin biomedical material
Method, this invention using tussah silk fibroin solution, three-D non-woven mesh material is prepared into by method of electrostatic spinning although
The permeability of support is beneficial to the growth of cell, but the degree of orientation due to fibrous fibroin albumen and degree of crystallinity are typically higher, lead
The cause vivo degradation cycle may be longer, is unfavorable for being applied to the shorter organization engineering skin support of degradation cycle.And the method
Using method of electrostatic spinning, spinning liquid and apparatus and process condition are required higher, be unfavorable for the performance of flexible product.
Dermal scaffold is sayed, the characteristic of material therefor to be taken into full account.Timbering material is markedly different from common engineering
Material, because it has a metabolic function, the clinical trial of Graftskin it was demonstrated that optimal timbering material should be with
What human body skin structure and composition composition was consistent has the albumen composite material of physiologically active, so that it is complicated to better adapt to skin
Physiological requirement.
Maltodextrin is a kind of starch derivatives of low-molecular-weight, is frequently used in food service industry and pharmaceutic adjuvant, its tool
Play the role of good moisture retention, nutritive value and promote immunocyte chemotactic, can promote the compatible of cell and storeroom and
The immunomodulating of wound（Referring to patent documentation CN104027346A）, beneficial to cell growth and wound healing.By maltodextrin with
Tussah silk fibroin is combined with each other, and not only can promote the adhesion of cell and the rehabilitation of wound, and component in preparation process
Maltodextrin serves the effect of porogen, by ultrasonic leaching dissolution hole forming technology scalable constituent content, porosity and toothed oak
Fibroin protein degree of crystallinity, makes the property regulation of compound rest simple, flexibly.
Pass through altogether with cyclodextrin in the patent documentation of 103223192A, describing a kind of Bombyx mori silk fibroin in notification number
Carry out cryodesiccated method after mixed and be prepared for a kind of composite for bone tissue engineer, so this material does not prove that ring is pasted
Essence has immune chemotactic activity.
The method of preparation organizational project porous material is a lot, has freeze-drying, porogen method（Salting out method, percolation）、
Foaming, method of electrostatic spinning, phase separation method etc..But, these pore methods are all pores, very flexible；And, at present
Porogen is not contained in prepared finished product in the porogen method reported, and often not degraded by environment, increase environmental burden.
As：The patent documentation of Publication No. CN105457096A discloses the tussah silk fibroin of a kind of good biocompatibility, degradable
The preparation method of tissue engineering bracket material, this invention describes freeze-drying, porogen method and three kinds of systems of method of electrostatic spinning
Preparation Method, but be all a pore, very flexible, and its degradable high polymer being blended with fibroin albumen is with synthetic polymer residence
Many, not only no nutrition and promote immunocompetence, and the Modulatory character of degradation cycle is difficult to meet clinical demand.Therefore it provides
A kind of compound support frame material for artificial skin having degradability and being easy to regulate and control has become this area skill urgently to be resolved hurrily
An art difficult problem.
Content of the invention
For the problems referred to above, the present invention provide that a kind of tussah silk fibroin degradation property is easy to regulate and control for organizational project
The preparation side that the active porous composite of dermal scaffold and its separated molding, pore and the secondary pore of ultrasonic leaching combine
Method, the present invention is realized in：
A kind of tussah silk fibroin be combined maltodextrin active scaffold material preparation method it is characterised in that concrete steps such as
Under：
（a）Prepare compound preparation solution：
Tussah cocoon shell after degumming is dissolved in calcium chloride and the compound aqueous solution of formic acid, after deionized water dialysis, concentrates
Mass concentration is the tussah silk fibroin aqueous solution of 8-10%, then adds maltodextrin in the solution, after dissolving is stirred at room temperature
Obtain compound preparation solution；
Wherein, in compound preparation solution, tussah silk fibroin and the mass ratio of maltodextrin are 5:1-4；
（b）Separated molding pore：
Compound preparation solution is immersed submergence 4-8h in phase transformation forming agent, makes compound preparation solution curing molding, be subsequently placed in 40 DEG C
It is vacuum dried in vacuum drying oven, obtained cavernous timbering material；
Described phase transformation forming agent is one of ethanol, acetone, propanol；
（c）The secondary pore of ultrasonic leaching：
Cavernous timbering material is immersed in ultrasonic immersion 10-60s in the ethanol water of volumetric concentration 20-50%, makes part
Maltodextrin dissolution, realizes second pore-forming of porous compound support frame, and reduces the degree of crystallinity of material, then material is placed in 40
DEG C vacuum drying oven in be vacuum dried, that is, obtain described tussah silk fibroin be combined maltodextrin active scaffold material.
（d）Preserve：Described tussah silk fibroin is combined maltodextrin active scaffold material and is placed in the sealing of vacuum seal pocket,
It is placed in 0 ~ 8 DEG C of refrigerator and preserves, and reduce the degree of crystallinity of material,
Further, tussah silk fibroin of the present invention is combined in the preparation method of maltodextrin active scaffold material, step（a）
Tussah silk fibroin mean molecule quantity in middle tussah silk fibroin aqueous solution is 20-200KDa.
Further, tussah silk fibroin of the present invention is combined in the preparation method of maltodextrin active scaffold material, step
Suddenly（a）The DE value of middle maltodextrin is 10-20.
Further, tussah silk fibroin of the present invention is combined in the preparation method of maltodextrin active scaffold material, step
Suddenly（a）In described compound preparation solution, tussah silk fibroin and the mass ratio of maltodextrin are 11-171:9.
Further, tussah silk fibroin of the present invention is combined in the preparation method of maltodextrin active scaffold material, step
Suddenly（a）Described maltodextrin is medicinal maltodextrin.
Further, tussah silk fibroin of the present invention is combined in the preparation method of maltodextrin active scaffold material, step
Suddenly（c）Ultrasonic power is 25-40KHz..
Further, tussah silk fibroin of the present invention is combined in the preparation method of maltodextrin active scaffold material, step
Suddenly（c）The porosity of the tussah silk fibroin base activity compound support frame material obtaining is 75-95% for porosity.
Further, tussah silk fibroin of the present invention is combined in the preparation method of maltodextrin active scaffold material, step
Suddenly（c）The aperture of the tussah silk fibroin base activity compound support frame material obtaining is 10-500mm.
Compared with prior art, the present invention has the advantages that：
1st, the present invention is compounded with the maltodextrin of low-molecular-weight in tussah silk fibroin base timbering material.Maltodextrin is a kind of
Polysaccharide starch derivatives, are also a kind of neutrophils chemotactic agent.By controlling its consumption and pore stripping quantity, both can adjust
The composition of section compound rest and performance, can play the effect of porogen again, also have certain cytotrophy effect and immunity
Adjustment effect.
2nd, step of the present invention（a）Compound preparation solution is separated after being shaped to porous material in organic coagulating bath, uses
The secondary hole forming technology of ultrasound wave leaching.The method is conducive to improving porosity and the permeability of timbering material；Meanwhile, the party
Method can adjust the degree of crystallinity of tussah silk fibroin in semi-finished product compound rest by ultrasonic leaching time length, not only improve and prop up
The degradation cycle of frame material and wound-healing cycle match, again can flexible support performance, shorten traditional preparation methods
It is formulated into the production cycle of finished product manufacture from solution.
3rd, the component of the compound support frame material of the present invention derives from natural macromolecular, all can be absorbed with vivo degradation, raw
The thing compatibility is good.And be starch derivatives it is easy to environment degradable, no environmental protection are born as the porogen maltodextrin of one of component
Load.
4th, the preparation technology of the present invention is more simple and environmentally-friendly, low toxicity, low energy consumption, with short production cycle, is conducive to carrying out industrialization
Batch production and emergent production.
Specific embodiment
By the following examples the technology of the present invention thought is described further, involved reagent and material in embodiment
Material, unless stated otherwise, is all to be bought by commercial sources to obtain.
Used in embodiment, medicinal maltodextrin is purchased from Shandong Xiwang Pharmaceutical Co., Ltd., and DE value is 10-20.
Embodiment 1
1st, prepare compound preparation solution：
Antherea pernyi Guerin-Meneville dried cocoon shell is boiled removing sericin through the sodium carbonate of 0.02M, then boiled-off cocoon shell is dissolved in mol ratio successively
For 1：3:In 15 calcium chloride, formic acid, the ternary mixed solution of water, molecular cut off be 12000 dialyzer in through go from
After the dialysis of sub- water, filter and remove insoluble impuritiess, concentrated obtain the tussah silk fibroin aqueous solution that mass ratio is 8%；Then,
Medicinal maltodextrin powder is dissolved in above-mentioned solution, the compound preparation solution of the acquisition that stirs.
In compound preparation solution, tussah silk fibroin and the mass ratio of medicinal maltodextrin are 11：9.
2nd, be separated molding, pore：
10mL is combined preparation solution and pours Φ 35mm × 12mm into（Diameter x height）In mould, it is subsequently placed in molding in ethanol, pore
6h, is subsequently placed in 40 DEG C of vacuum drying ovens and is dried, that is, obtain cavernous timbering material.
3rd, ultrasonic secondary leaching pore：
Cavernous timbering material immersion volumetric concentration is ultrasonic 60s in 50% ethanol water, frequency 25kHz, with molten
Solution part maltodextrin, improves porosity, reduces degree of crystallinity；It is subsequently placed in 40 DEG C of vacuum drying ovens and is dried, that is, obtain described
Tussah silk fibroin base activity compound support frame material.
4th, by the i.e. acquisition described tussah silk fibroin base obtaining activity compound support frame material, it is sealed in vacuum bag, ice
Case cryopreservation.
Embodiment 2
1st, prepare compound preparation solution：
Antherea pernyi Guerin-Meneville dried cocoon shell is boiled removing sericin through the sodium carbonate of 0.02M, then boiled-off cocoon shell being dissolved in mol ratio is 1：
2:In 12 calcium chloride, formic acid, the ternary mixed solution of water, molecular cut off be 12000 dialyzer in through deionized water
After dialysis, filter and remove insoluble impuritiess, concentrated obtain the tussah silk fibroin aqueous solution that mass fraction is 8%.Then, will
Maltodextrin powder is dissolved in above-mentioned solution, is prepared into compound preparation solution.
In compound preparation solution, tussah silk fibroin and the mass ratio of medicinal maltodextrin are 100：9.
2nd, be separated molding, pore：
10mL is combined preparation solution pour in Φ 35mm × 12mm mould, then inserts molding in acetone, pore 4h, 40 DEG C of vacuum
It is dried.
3rd, ultrasonic secondary leaching pore：
The cavernous timbering material obtaining after 40 DEG C are vacuum dried, immersion volumetric concentration is in 30% ethanol water, surpasses
Sound 30s, supersonic frequency 28kHz, dissolves part maltodextrin, then material is placed in 40 DEG C of vacuum drying ovens and is dried, that is,
Obtain described tussah silk fibroin base activity compound support frame material.
Embodiment 3
1st, prepare compound preparation solution：
Antherea pernyi Guerin-Meneville dried cocoon shell is boiled removing sericin through the sodium carbonate of 0.02M, then boiled-off cocoon shell being dissolved in mol ratio is 1：
3:In 12 calcium chloride, formic acid, the ternary mixed solution of water, molecular cut off be 3500 dialyzer in saturating through deionized water
After analysis, filter and remove insoluble impuritiess, concentrated obtain the tussah silk fibroin aqueous solution that mass ratio is 10%；Then, by Fructus Hordei Germinatus
Dextrine powder is dissolved in above-mentioned solution, then be prepared into compound preparation solution.
In compound preparation solution, tussah silk fibroin and the mass ratio of medicinal maltodextrin are 171：9.
2nd, be separated molding, pore：
5mL is combined preparation solution pour in Φ 35mm × 12mm mould, then inserts molding in propanol, pore 8h, then 40 DEG C
Cavernous timbering material is obtained after vacuum drying.
3rd, ultrasonic secondary leaching pore：
The cavernous timbering material immersion volumetric concentration obtaining is ultrasonic 10s in 20% ethanol water, frequency
40kHz, dissolves part maltodextrin, is subsequently placed in 40 DEG C of vacuum drying, that is, obtain described tussah silk fibroin base activity multiple
Close timbering material.
In specific operation process, in compound preparation solution, tussah silk fibroin can also be in 11- with the mass ratio of maltodextrin
171:Select in the range of 9, all can achieve the purpose of the present invention.
Claims (9)
1. a kind of tussah silk fibroin is combined the preparation method of maltodextrin active scaffold material it is characterised in that concrete steps
As follows：
（a）Prepare compound preparation solution：
By tussah silk peptide dried cocoon shell after the sodium carbonate liquor of 0.02M boils 30min degumming, it is dissolved in the compound of calcium chloride and formic acid
In aqueous solution, after deionized water dialysis, it is concentrated to give the tussah silk fibroin aqueous solution that mass concentration is 8-10%, then
Maltodextrin is added in above-mentioned solution, obtains the compound preparation solution of tussah silk fibroin and maltodextrin；
（b）Separated molding pore：
Compound preparation solution is immersed in phase transformation forming agent, submergence 4-8h, it is subsequently placed in 40 DEG C of vacuum drying, obtain cavernous
Frame material；
Described phase transformation forming agent is one of ethanol, acetone, propanol；
（c）The secondary pore of ultrasonic leaching：
Cavernous timbering material is immersed in ultrasonic immersion 10-60s in the ethanol water that volumetric concentration is 20-50%, then
It is placed in 40 DEG C of vacuum drying, that is, obtain described tussah silk fibroin and be combined maltodextrin active scaffold material.
2. tussah silk fibroin is combined the preparation method of maltodextrin active scaffold material, its feature according to claim 1
It is, described step（a）Tussah silk fibroin mean molecule quantity in middle tussah silk fibroin aqueous solution is 20-200KDa.
3. tussah silk fibroin is combined the preparation method of maltodextrin active scaffold material, its feature according to claim 2
It is, step（a）The DE value of middle maltodextrin is 10-20.
4. it is combined the preparation method of maltodextrin active scaffold material according to the described tussah silk fibroin of one of claim 1-3,
It is characterized in that, in the compound aqueous solution of described calcium chloride and formic acid, the mol ratio of calcium chloride, formic acid and water is followed successively by 1：2-3:
12-15.
5. it is combined the preparation method of maltodextrin active scaffold material according to the described tussah silk fibroin of one of claim 1-3,
It is characterized in that, step（a）In described compound preparation solution, tussah silk fibroin is 11-171 with the mass ratio of maltodextrin:9.
6. it is combined the preparation method of maltodextrin active scaffold material according to the described tussah silk fibroin of one of claim 1-3,
It is characterized in that, step（a）Described maltodextrin is medicinal maltodextrin.
7. tussah silk fibroin is combined the preparation method of maltodextrin active scaffold material, its feature according to claim 6
It is, step（c）Ultrasonic power is 25-40KHz.
8. it is combined the preparation method of maltodextrin active scaffold material according to the described tussah silk fibroin of one of claim 1-3,
It is characterized in that, step（c）The porosity of the tussah silk fibroin base activity compound support frame material obtaining is 75-95%.
9. tussah silk fibroin is combined the preparation method of maltodextrin active scaffold material, its feature according to claim 8
It is, step（c）The aperture of the tussah silk fibroin base activity compound support frame material obtaining is 10-500mm.
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| CN108070106A (en) * | 2017-11-22 | 2018-05-25 | 玖久丝绸股份有限公司 | A kind of preparation method of fibroin albumen grafting maltodextrin porous material |
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| US20070065481A1 (en) * | 2005-09-21 | 2007-03-22 | Chudzik Stephen J | Coatings including natural biodegradable polysaccharides and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108070106A (en) * | 2017-11-22 | 2018-05-25 | 玖久丝绸股份有限公司 | A kind of preparation method of fibroin albumen grafting maltodextrin porous material |
| CN108070106B (en) * | 2017-11-22 | 2020-06-30 | 玖久丝绸股份有限公司 | Preparation method of silk fibroin grafted maltodextrin porous material |
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