CN106471356B - Automatic analysing apparatus - Google Patents
Automatic analysing apparatus Download PDFInfo
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- CN106471356B CN106471356B CN201580033562.9A CN201580033562A CN106471356B CN 106471356 B CN106471356 B CN 106471356B CN 201580033562 A CN201580033562 A CN 201580033562A CN 106471356 B CN106471356 B CN 106471356B
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- Prior art keywords
- light
- reaction vessel
- measure
- light source
- port
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
Abstract
Due to implementing the inspection of light-source brightness before the assay to ensure the higher measure of reliability, so needing new additional detector, need to add amplifier, A/D change-over circuits for this.The present invention provide a kind of automatic analysing apparatus it possess a part of light from the light source when not setting reaction vessel to measure port and incide test section, when reaction vessel to be set to the light from light source when determining port to the optical texture that is not incident on the test section.
Description
Technical field
The present invention relates to the automatic analysing apparatus that a kind of component amount included to blood plasma and serum equal samples is analyzed,
A kind of more particularly to automatic analysing apparatus for determining blood coagulation time project.
Background technology
A kind of known automatic analysing apparatus, it, which is determined, makes the light from light source be shone to the reaction solution for being mixed with sample and reagent
The transmission light quantity or amount of scattered light of single or multiple wavelength obtained from penetrating, sample is calculated further according to the relation of light quantity and concentration
Comprising component amount.
In automatic analysing apparatus, the device of the solidification function of measure blood also be present.Although blood is kept in the blood vessels
Mobility is flowing, once but bleeding, coagulation factors present in blood plasma and blood platelet just activate by linksystem, so as to blood
Fibrinogen in slurry is converted into fibrin and separated out, untill hemostasis.
Exogen blood clotting function when escaping to EV blood clotting in such blood clotting function be present
Endogenous blood clotting function when being solidified with vessel inner blood.Most exogen, endogenous are jointly summarised as fiber egg at last
White original is converted into the blood coagulation reaction of fibrinous intercommunity.As the measure project about blood clotting function, exist
Prothrombin time (PT), the activation partial thromboplastin time (APTT) of endogenous and the fibre of intercommunity of exogen
Fibrillarin commercial weight (Fbg) etc..
Each of these projects are carried out by the following method, i.e. make the reagent that solidification starts by addition to analyse
Go out fibrin, the fibrin of precipitation is detected with optical, physical, electrical resistance gimmick.As using optics
Property means method, it is known that following methods, i.e. make light be irradiated to reaction solution, by grasping by the fiber egg that is separated out in reaction solution
Transmitted light caused by white and scattering light through when time (setting time) for being separated out to calculate fibrin to start of Strength Changes.
As the device that setting time is determined using transmitted light, automatic analysing apparatus as patent document 1 be present.Specially
The automatic analysing apparatus of sharp document 1 makes the light of multiple wavelength be irradiated to reaction solution, generally according to having used short wavelength's (dominant wavelength)
High sensitivity light-metering obtain response curve, further according to obtained response curve setting time is calculated, when there is blood in sample
When pigment, bilirubin, milky white thing etc. interfere the influence of material, according to the reaction based on the higher long wavelength of transmittance (commplementary wave length)
Curve calculates setting time.Dominant wavelength is 660nm, a length of 800nm of complementary wave in PT, APTT, and dominant wavelength uses in Fbg
405nm, commplementary wave length have used 660nm.Due to being transmission light measurement, so real-time for that can be carried out to the light-source brightness before measure
The system of inspection.
The device of setting time is determined as using scattering light, it is known that automatic analysing apparatus as patent document 2.Specially
The automatic analysing apparatus of sharp document 2 uses, and the influence of hemochrome and milky white thing is less in interference material, wavelength ratio is shorter
660nm light source.Although the measure of single wavelength, as a result of the scattering in higher sensitivity compared with transmission light measurements
Photo measure, so high sensitivity and dynamic range can be taken into account.However, in order to check the light-source brightness before measure, need
Detector beyond the detector of measure is set.
The optical system of patent document 3 is the optical system of scattered light measuring, is also had in addition to the detector of measure
It is ready for use on the detector of benchmark actinometry.
Prior art literature
Patent document
Patent document 1：Japanese Patent Laid-Open 2007-263912 publications
Patent document 2：Japanese Patent Laid-Open 2000-321286 publications
Patent document 3：Japanese Patent Laid-fair 1-22575 publications
The content of the invention
The technical problems to be solved by the invention
According to patent document 2 automatic analysing apparatus like that by it is highly sensitive scattering photo measure to be solidified when
Between project measure structure, then can take into account high sensitive and dynamic range with single wavelength, so as to simplify optical system
System, therefore it is capable of the reduction of realization device cost.However, due to implementing before the assay to ensure the higher measure of reliability
The inspection of light quantity, so needing newly to add detector as described in Patent Document 3, need to add amplifier and A/D conversion electricity for this
Road, so as to which installation cost can rise.
Technical scheme used by solution technical problem
The representational invention of present patent application is listed below.
A kind of automatic analysing apparatus, possesses：Reaction vessel, it possesses lens arrangement in bottom；Reaction vessel transfer mechanism,
It transfers reaction vessel；Sample dispenses mechanism, and it dispenses sample to reaction vessel；Reagent dispenses mechanism, and reagent is dispensed into by it
Reaction vessel, there is reagent heating function；Light source, it is used for the reaction solution for making light from reaction vessel bottom direction into reaction vessel
Irradiation；Port is determined, it possesses the detector detected to the scattering light from the reaction solution being irradiated by light, described to divide automatically
Analysis apparatus is characterised by possessing one when not setting the reaction vessel to the measure port from the light source
The test section is incided in light splitting, when the reaction vessel is set to the measure port, the light from the light source does not enter
It is mapped to the optical texture of the test section.
Because only when the reaction vessel that bottom possesses lens arrangement is set on detector, the light from light source passes through
Lens arrangement and be condensed to reaction solution, again can be so be both the time of setting test optical system that make use of scattering photo measure
It is not provided with the light quantity inspection of the measure advance line light source of reaction vessel.According to this structure, due to being used for light quantity inspection without addition
New detector and circuit, so can restraining device cost rising, and it is reliable so as to carry out to carry out light quantity detection
The higher time of setting test of property.
Invention effect
It is an object of the present invention to provide a kind of automatic analysing apparatus, the rising of its restraining device price, and can carry out
Light quantity inspection is so as to carrying out the time of setting test of high reliability.
Brief description of the drawings
Fig. 1 is the skeleton diagram of the automatic analysing apparatus as an embodiment of the present invention.
Fig. 2 a are that the reaction for possessing lens arrangement in the automatic analysing apparatus as an embodiment of the present invention in bottom is held
The profile of device.
Fig. 2 b are that possess the profile to form convex form in the automatic analysing apparatus as an embodiment of the present invention in bottom
Lens arrangement reaction vessel profile.
Fig. 3 is that reaction vessel is set into the section to the warm adjustable block in the state of measure port in an embodiment of the present invention
Figure.
Fig. 4 is to show to be not provided with determining in port in the state of reaction vessel in an embodiment of the present invention to come from light
The figure of the irradiating state of the light in source.
Fig. 5 is to show that there is provided determine in port to come from light in the state of reaction vessel in an embodiment of the present invention
The figure of the irradiating state of the light in source.
Fig. 6 be show in an embodiment of the present invention be provided with benchmark scatterer in the state of determine port in,
The irradiation of light from light source and from reaction solution scattering light generation state figure.
Fig. 7 is the section that the light quantity being made up of in an embodiment of the present invention translucent high polymer material checks component
Figure.
Fig. 8 is the figure for the flow chart for showing light quantity inspection when device starts in an embodiment of the present invention.
Fig. 9 is the figure for the flow chart for showing the light quantity inspection before being determined in an embodiment of the present invention.
Figure 10 is temporarily ceased with base when being and showing and set reaction vessel to measure port in an embodiment of the present invention
Implement the figure of the action of the light quantity inspection before measure in the light that reaction vessel bottom is reflected.
Figure 11 is to show that the bias light set in an embodiment of the present invention based on the top for coming comfortable measure port is hidden
The reflected light of the light source light of light Optic gate come implement measure before light quantity inspection an example figure.
Figure 12 is to show the measure for possessing the reaction vessel fixing spring with reflecting plate in an embodiment of the present invention
The front cross-sectional view (a) of port, side cut away view (b) and there is provided the state (c) of reaction vessel in the measure port
Figure.
Embodiment
Hereinafter, based on accompanying drawing, embodiments of the present invention are specifically described.Furthermore for illustrating this embodiment party
In all accompanying drawings of formula, to marking identical label on the Some principles with identical function, its repeat specification is omitted as far as possible.
Fig. 1 is the skeleton diagram of the automatic analysing apparatus as one embodiment of the invention.As shown in figure 1, the present invention's is automatic
Analytical equipment includes：Sample container 1 is configured into sample disk 2 circumferentially；The sample dispensing machine of sample is dispensed to reaction vessel 7
Structure 3；It is provided with the reagent disk 5 of reagent bottle 4；The reagent for the band heating function that reagent is dispensed to reaction vessel dispenses mechanism
6；The reaction vessel receiving portion 8 of multiple reaction vessels 7 used in the assay is laid in；For transferring the reaction of reaction vessel 7
Container transfer mechanism 9；Sample for dispensing sample dispenses port 10；Possesses the warm adjustable block 12 of multiple measure ports 11；It will survey
The reaction vessel waste part 13 that reaction vessel 7 after terminating calmly is discarded；Control unit 14；Storage part 15；For carrying out picture expression
Expressed portion 16；And the input unit 17 for entering the operating instructions etc..
It is briefly described for the control system in Fig. 1 automatic analysing apparatus and signal processing system.Control unit
Control, the control of measure order that 14 are substantially carried out the controlling of mechanism action, temperature is adjusted.The close of each operator is stored in storage part 15
Code, the expression grade of each picture, analytical parameters, analysis project require the information such as content, calibration result, analysis result.
Then, the analysis action to the sample in Fig. 1 automatic analysing apparatus illustrates.It is relevant by automatic analysing apparatus
The analytical parameters of analyzable project, it is to be inputted in advance via input unit 17, is stored in storage part 15.Operator makes
The inspection project to each sample requirement is selected with the operating function picture of expressed portion 16.Now, also by the information such as patient ID from
Input unit 17 inputs.
Circumferentially being put on sample disk 2 has the multiple physical containers 1 for accommodating the biopsy samples such as blood plasma, serum, is illustrating
Example in circumferentially put two layers of physical container 1.In addition, sample dispensing mechanism 3 is configured near sample disk 2.This sample
Liquid relief mouth of this dispensing mechanism 3 mainly by moveable arm and on moveable arm is formed.Sample dispenses mechanism 3 to analyze pair
The inspection project that each sample is specified, and liquid relief mouth is moved to dispensing position by movable wall Lai appropriate, then from positioned at sample disk 2
Suction position physical container 1 in, aspirate according to analytical parameters the sample of ormal weight, then to by reaction vessel transfer mechanism 9
Pre-set discharge sample in the reaction vessel 7 in sample dispensing port 10.
The reaction vessel 7 that dispensing has sample is arranged in determination part 11 by reaction vessel transfer mechanism 9.Possess multiple surveys
The warm adjustable block 12 of fixed end mouth 11 is controlled at certain temperature (such as 37 DEG C) by control unit 14.Make reaction vessel 7 in warm adjustable block 12
At the time of interior sample is changed into defined temperature, mechanism 6 is dispensed come according to appropriate inspection project by the reagent with heating function
Analytical parameters reagent is aspirated from the set reagent disk 4 being arranged in reagent disk 5, the reagent of extraction is raised to both constant temperatures
After spending (such as 39 degree), then spued into reaction vessel 7.Now, due to reagent discharge impulse force, the stirring of sample and reagent
It is carried out, so as to which blood sample coagulation reaction starts.Reaction vessel 7 after reaction terminates is discarded by reaction vessel transfer mechanism 9
To reaction vessel waste part 13.Component concentration data as the analysis result of each inspection project is output to expressed portion 16.
It is described below on time of setting test.Before reaction vessel 7 is set into measure port 11, light is confirmed
The inspection light quantity Ic in source.When checking that light quantity Ic is in given area, start the measure in the measure port 11.By reaction vessel
The reaction vessel 7 that dispensing has sample is set the measure port 11 by transfer mechanism 9, thereafter again by the reagent point with heating function
Note mechanism 6 and dispense reagent to the reaction vessel 7.Light is set to have sample and the reaction vessel of reagent 7 to irradiate from light source to dispensing, by surveying
The detector 19 of fixed end mouth 11 receives scattering light.The signal of light income come amplification, is converted to by amplifier by A/D converter
The numerical value proportional to light quantity, then it is stored in storage part 15 via control unit 14.Using the numerical value after this conversion, by be each
Analytic approach that inspection project is specified calculates setting time.According to inspection project, according to the setting time calculated, based on inspection line
To calculate concentration data etc., check that line is to be obtained by the analytic approach specified for each inspection project and measured in advance.
Fig. 2 a show the reaction vessel 7 for possessing lens arrangement in the automatic analysing apparatus of an embodiment of the present invention in bottom
Profile.Fig. 2 b are the profiles of the reaction vessel 7 for the lens arrangement for possessing the profile to form convex form in bottom.As anti-
The material of container 7 is answered, transparent material is preferred, but can also apply translucent material.In addition, in columnar reaction vessel
To be configured with the structure of lens arrangement be preferred for bottom, but it is also contemplated that the reaction vessel of polygon and lens arrangement are configured
To the embodiment of reaction vessel side.However, the reaction vessel of polygon and lens arrangement are being configured to reaction vessel side
In the case of, need to consider the setting direction of reaction vessel 7 when reaction vessel 7 is set into measure port 11.
Shown in Fig. 3 by reaction vessel 7 set measure port 11 in the state of warm adjustable block 12 profile.Warm adjustable block
12 comprising it is multiple measure ports 11, it is each measure port mainly include be used for by light from reaction vessel bottom direction into reaction vessel
Reaction solution irradiation light source 18 and the detector 19 that is detected to the scattering light from the reaction solution being irradiated by light.Again
Person, eliminate the diagram to thermoregulation mechanism.Become following structure, i.e. by light source by light from the bottom side of reaction vessel 7
To irradiation, by the bottom lens structure of reaction vessel 7 and optically focused, then received with the detector 19 set in the two directions
Scatter light.The light that light, the detector 19 irradiated from light source 18 is received is limited by light projector aperture section, reception aperture section respectively.
When reaction vessel 7 is set to measure port 11, because the convex form of the bottom lens structure of reaction vessel 7 is and light projector light
The identical shape in circle portion opening portion, so the position of lens arrangement can be determined, so as to determine reaction vessel 7 setting measure
The position of port 11.The position of reaction vessel 7 so is determined by making convex form coincide to light projector aperture section opening portion, so as to
Higher precision and the higher scattering photo measure of reliability can be carried out.
Fig. 4 shows to be not provided with to determine the irradiating state of the light from light source 18 in port 11 in the state of reaction vessel 7.
Become following structure, i.e. due to the bottom lens structure of no reaction vessel 7, therefore the light irradiated from light source 18 has
Range, a part of irradiation light incide detector 19.Thereby, it is possible to the inspection light quantity Ic to the Index for examination as light-source brightness
It is measured.So, automatic analysing apparatus 1 possesses comes from the light source when reaction vessel 7 is not set into measure port 11
18 a part of light incides test section 19, when reaction vessel 7 is set into measure port 11, the light from light source 18 does not enter
It is mapped to the optical texture of test section 19.
Show that there is provided the irradiation shape of the light from light source 18 determined in the state of reaction vessel 7 in port 11 in Fig. 5
State.Become following structure, i.e. do not enter and optically focused from the light that light source 18 irradiates by the bottom lens structure of reaction vessel 7
It is mapped to detector 19.So, by the way that the light of light source is carried out into optically focused, the signal based on scattering light can be amplified.In addition, pass through by
The liquid level that focus is set in reaction solution nearby come limit direct light to react liquid level range of exposures, so as to reduce by
React the influence of reflected light and scattering light caused by the meniscus and bubble of the inwall generation of liquid level and reaction vessel 7.Therefore, it is excellent
Selection of land, optical texture and lens arrangement are designed so that by the lens arrangement of the bottom of reaction vessel 7 and caused light source
The focus of 18 light is set near the liquid level in the reaction solution in reaction vessel.
Show to be provided with the state of dispensing has the reaction vessel 7 of benchmark scatterer 20 in Fig. 6 and determine in port 11
The irradiation of light from light source 18 and the generation state for scattering light from reaction solution.The light irradiated from light source 18 is by reaction vessel
7 bottom lens structure optically focused, is irradiated on benchmark scatterer 20, so as to produce scattering light.Detector 19 receives this scattering
Light, to determine the benchmark amount of scattered light Is of light-source brightness inspection.Benchmark scatterer 20 is for example it is contemplated that latex particle mixes
Turbid etc., but as long as being the material for sending stable scattering light, it is not particularly limited.Also can apply by translucent macromolecule material
Expect the scattering object of the solid of composition.
The profile of light quantity inspection component 21 being made up of translucent high polymer material is shown in Fig. 7.Light quantity inspection
It is the shape for having imitated reaction vessel 7 with component 21, can be transferred by reaction vessel transfer mechanism 9 to measure port 11.Pass through
Will the light quantity to check that the receiving port of component 21 is set stable in temperature to that in warm adjustable block 12, can make, so as to reduce by
Benchmark amount of scattered light Is deviation caused by thermal deformation etc..In addition, by making connecing for the light source irradiation light of light quantity inspection component 21
Receipts portion and to the shape that the light-projecting portion of detector 19 is profile indent, so as to prevent from being drawn by the friction injury with determining port 11
The light quantity risen changes.It is therefore preferred that the light quantity check component 21 light source light receiver and scattering light light-projecting portion relative to
Peripheral shape forms concave shape.Due to light quantity inspection component 21 can Reusability, so with use benchmark scattering
The situation of matter 20 is compared, and can reduce life cycle cost.
The flow chart of light quantity inspection when device starts is shown in Fig. 8.Content in this flow chart is by control unit 14 real
OK.The light quantity inspection of light source 18 when the implementation of control unit 14 starts.
At the time of the light quantity from light source 18 after starter power supply reaches stable, light quantity inspection (figure is automatically begun to
8a).Reaction vessel 7 is set into measure port 11 by reaction vessel transfer mechanism 9, the reagent with heating function dispenses mechanism 6
One of the reagent bottle 4 set in reagent disk 5 the benchmark scatterer 20 filled is aspirated into ormal weight, then to the reaction vessel
7 spue (Fig. 8 b).Thus, the reaction vessel for accommodating benchmark scatterer is provided with port 11 is determined.
At the time of the temperature of benchmark scatterer 20 reaches stable, benchmark amount of scattered light Is (Fig. 8 c) is determined.To benchmark
Whether amount of scattered light Is in given area set in advance is confirmed (Fig. 8 d), and additional basis scatters when not in the range of
Light quantity Is abnormality warnings (Fig. 8 e), light source state is confirmed to require to represent in expressed portion 16 (Fig. 8 n).As benchmark amount of scattered light Is
When in given area, the variation relative to previous value is calculated, to confirm to change whether in given area.When variation was not both
When determining in scope, additional basis amount of scattered light Is changes warning (Fig. 8 g), and light source state is confirmed to require to represent in expressed portion 16
(Fig. 8 n).When benchmark amount of scattered light Is variation is in given area, by the benchmark amount of scattered light Is determined storages to depositing
Storage portion 15 (Fig. 8 h).The reaction that the benchmark scatterer 20 after terminating is determined equipped with benchmark amount of scattered light Is in one measure port
Container 7 is moved to next measure port 11 (Fig. 8 i) by reaction vessel transfer mechanism 9, similarly implements benchmark amount of scattered light Is
Measure.So, the step of control unit 14 carries out the step of light quantity inspection (the step of the first light quantity checks), and the light quantity checks profit
With the scattered light measuring result based on the benchmark scatterer set in port 11 is determined.An example is above are only, as long as
It is the light quantity inspection for used the scattered light measuring result based on scattering primary standard substance, can also be not necessarily above-mentioned specific
Example.
Then, reference inspection light quantity Ics (Fig. 8 j) is surveyed in the state of reactionless container 7 in determining port 11
It is fixed, then confirm reference inspection light quantity Ics whether in given area (Fig. 8 k).When reference inspection light quantity Ics is not in given area
When interior, additional basis checks light quantity Ics abnormality warnings, and light source state is confirmed to require to represent in expressed portion 16 (Fig. 8 n).Work as base
When standard checks that light quantity Ics is in given area, storage part 15 (Fig. 8 m) is arrived into reference inspection light quantity Ics storages, when device starts
Light quantity inspection terminate.So, control unit 14 carries out the step of light quantity inspection (the step of the second light quantity checks), the light quantity inspection
The step of to make use of in measure port 11 be the result that is measured to a part for the light from light source in the state of empty.It is above-mentioned
An only example, a part for the light from light source is surveyed in the state of sky is in measure port as long as used
The light quantity inspection of fixed result, can also be not necessarily above-mentioned concrete example.
With such flow, using as the benchmark amount of scattered light of the amount of scattered light measurement result based on benchmark scatterer 20
Storage part 15 is arrived in Is and the reference inspection light quantity Ics storages determined immediately after, so as to as the light quantity before measure
The benchmark of inspection uses.In the case of substituting benchmark scatterer 20 checking component 21 using light quantity, it can also apply
Flow shown in Fig. 8.
The flow chart of light quantity inspection before measure is shown in Fig. 9.Content in this flow chart is carried out by control unit 14.Control
Implement the light quantity inspection of the light source 18 before determining in portion 14 processed.
At the time of reaction vessel 7 are not present in measure port 11 before time of setting test will start, start to determine
Preceding light quantity inspection (Fig. 9 a).In the state of reaction vessel 7 is not provided with determining port 11, to checking that light quantity Ic carries out light-metering
(Fig. 9 b), then whether the inspection light quantity Ic for confirming to determine is in given area (Fig. 9 c).When inspection light quantity Ic is in given area
When outer, additional examination light quantity Ic abnormality warnings (Fig. 9 d), the measure port 11 is shielding for unusable (Fig. 9 g), by light source shape
State confirms to require to represent in expressed portion 16 (Fig. 9 h).When check light quantity Ic in given area when, to check light quantity Ic relative to
Whether reference inspection light quantity Ics variation is checked (Fig. 9 e) in given area.When inspection light quantity Ic variation was both
When determining outside scope, additional examination light quantity Ic changes warning, the measure port 11 is shielding for into unusable (Fig. 9 g), by light source shape
State confirms to require to represent in expressed portion 16 (Fig. 9 h).When checking that light quantity Ic variation is in given area, light quantity is examined before measure
Normal termination (Fig. 9 i) is looked into, starts the time of setting test in the measure port 11 thereafter.So, control unit 14 is deposited by comparing
The result being measured in the state of sky is in measure port to a part for the light from light source stored in storage portion (examine by benchmark
Look into light quantity Ics), with being measured before will determining in measure port to a part for the light from light source in the state of empty
Result (check light quantity Ic), to implement the light quantity inspection of light source.
In the present embodiment, start all to checking component based on benchmark scatterer 20 or light quantity although employing each device
21 benchmark amount of scattered light Is implements the form of measure, but it is also contemplated that only when device manufacture to benchmark amount of scattered light Is with
Reference inspection light quantity Ics is measured and is prestored to storage part 15, so as to the shape for light quantity inspection before later measure
State.
It is described herein below on the other method for carrying out light-metering to reference inspection light quantity Ics and inspection light quantity Ic.
Show to temporarily cease with the bottom based on reaction vessel 7 when reaction vessel 7 is set to measure port 11 in Figure 10
The light reflected implements an example of the action of light quantity inspection.When the reaction vessel as possessed by reaction vessel transfer mechanism 9
Grip part 22 by reaction vessel to measure port 11 set when, can be detected in the reflected light in the bottom of reaction vessel 7
Temporarily ceased in the height that device 19 receives, to be measured to reference inspection light quantity Ics and inspection light quantity Ic.Now, Neng Gouye
Implement the confirmation of the setting to reflection containers 7 in the lump.
It is preferred that such scheme, i.e. when reaction vessel 7 is arranged at measure port 11 by reaction vessel transfer mechanism 9, instead
Answer container transfer mechanism 9 that reaction vessel 7 is held in into the light source reflected light from reaction container bottom and incide test section 19
On position, and stop certain time in this condition, control unit 14 implements the measure in the step of the second light quantity checks.
Show to implement the light quantity inspection before measure based on the reflected light of the light source 18 reflected by shading Optic gate 23 in Figure 11
The example looked into.Although larger light quantity can not be expected for the reflected light reflected by shading Optic gate 23, due to that can reduce
The shift in position of shading Optic gate 23, so the preferable light quantity inspection of repeatability becomes possibility.In addition, by shading Optic gate
Reflecting plate is set on 23, so as to also set reflecting plate on the measure top of port 11.
It is preferred that such scheme, i.e. the light source reflected light based on the reflecting plate 23 for carrying out the configuration of the comfortable measure top of port 11,
Control unit 14 implements the measure in the step of the second light quantity checks.Additionally, it is preferred that such scheme, i.e. reflecting plate has measure concurrently
The function of shading Optic gate 23, i.e. exterior light shadow shield.As long as at least set in the measure port side of shading Optic gate 23
Reflecting plate.
Show to possess in Figure 12 the reaction vessel fixing spring with reflecting plate measure port front cross-sectional view (a),
Side cut away view (b) and the state (c) that reaction vessel 7 is provided with the measure port.When determining areflexia in port 11
During container 7, the reflection containers fixed spring 24 with reflecting plate significantly ejects forward, can use in the anti-of spring at front end configuration
The reflected light for the light source 18 that plate is reflected is penetrated, to implement the light quantity inspection of enough light quantities.In addition, when there is provided during reaction vessel 7,
Reflection containers fixed spring 24 with reflecting plate is accommodated in reaction vessel fixed spring receiving portion 25, and due to securing
Reaction vessel 7 is so stable measure can be realized.Furthermore, can also as long as reflecting plate can be ejected into stable position forward
Not in the front end of spring.
It is preferred that such scheme, i.e. measure port 11 possesses the leaf spring of fixed reaction vessel 7, and the leaf spring possesses reflection
Plate, when reaction vessel 7 is not set into measure port 11, the reflecting plate is positioned to make the light from light source towards inspection
The position of survey portion reflection, control unit 14 implement the measure in the step of the second light quantity checks.
Furthermore in the example illustrated by above-mentioned Figure 10~12, come when not setting reaction vessel to measure port
Being not incident on from the light of light source in the optical texture of test section can also apply.In addition, in reaction vessel without lens mechanism
Reaction vessel, can also apply.
Label declaration
1 sample container
2 sample disks
3 samples dispense mechanism
4 reagent bottles
5 reagent disks
The 6 reagent dispensing mechanisms with heating-up mechanism
7 reaction vessels
8 reaction vessel receiving portions
9 reaction vessel transfer mechanisms
10 samples dispense port
11 measure ports
12 temperature adjustment blocks
13 reaction vessel waste parts
14 control units
15 storage parts
16 expressed portions
17 input units
18 light sources
19 detectors
20 benchmark scatterers
21 light quantities check component
22 reaction vessel grip parts
23 shading Optic gates
The 24 reflection containers fixed springs with reflecting plate
25 reaction vessel fixed spring receiving portions
Claims (12)
1. a kind of automatic analysing apparatus, possesses：
Reaction vessel, it possesses lens arrangement in bottom；Reaction vessel transfer mechanism, it transfers reaction vessel；Sample dispensing machine
Structure, it dispenses sample to reaction vessel；Reagent dispenses mechanism, and reagent is dispensed into reaction vessel by it, has reagent heating function；
Light source, it is used to irradiate reaction solution of the light from reaction vessel bottom direction into reaction vessel；Determine port, it possess to from
The test section that the scattering light for the reaction solution being irradiated by light is detected, the automatic analysing apparatus be characterised by,
Possess a part of light from the light source when not setting the reaction vessel to the measure port and incide institute
State test section, the light from the light source is not incident on the detection when the reaction vessel is set to the measure port
The optical texture in portion.
2. automatic analysing apparatus as claimed in claim 1, it is characterised in that
For the lens arrangement of the reaction vessel in profile of the bottom formed with convex form, the convex form is the throwing with the light source
The identical shape in light aperture section opening portion, by making the convex form coincide to the light projector aperture section opening portion, to described
Reaction vessel is positioned.
3. automatic analysing apparatus as claimed in claim 1 or 2, it is characterised in that
The optical texture and the lens arrangement be designed so that by the lens arrangement of the bottom of the reaction vessel and
The focus of the light of the caused light source is set near the liquid level in the reaction solution in the reaction vessel.
4. automatic analysing apparatus as claimed in claim 1 or 2, it is characterised in that
Control unit is also equipped with, the control unit implements the light quantity inspection of the light source,
The reaction vessel for accommodating benchmark scatterer is set in the measure port,
The step of the step of control unit is checked by the first light quantity and the inspection of the second light quantity, implements the light of the light source
The step of amount checks, first light quantity checks make use of based on the benchmark scatterer being arranged in the measure port
Scattered light measuring result, the step of second light quantity checks make use of the measure port in the state of empty to from
The result that a part for the light of light source is measured.
5. automatic analysing apparatus as claimed in claim 4, it is characterised in that
The light quantity that the benchmark scatterer is made up of translucent high polymer material checks component, can be by the reaction vessel
Transfer mechanism is transferred.
6. automatic analysing apparatus as claimed in claim 5, it is characterised in that
The light quantity checks that the light source light receiver of component and scattering light light-projecting portion form concave shape relative to peripheral shape.
7. automatic analysing apparatus as claimed in claim 4, it is characterised in that
Possesses storage part, storage part scattered light measuring result of the storage based on the benchmark scatterer and in the measure
Port is the result that is measured to a part for the light from light source in the state of empty,
These results that the control unit is stored according to the storage part, to implement the light quantity inspection of the light source.
8. automatic analysing apparatus as claimed in claim 7, it is characterised in that
The control unit in the storage part by storing in the state of sky is in the measure port to from light source
Light a part be measured result, with will determine before the measure port in the state of empty to from light source
The result that a part for light is measured, to implement the light quantity inspection of the light source.
9. automatic analysing apparatus as claimed in claim 4, it is characterised in that
When the reaction vessel is arranged at the measure port by the reaction vessel transfer mechanism, the reaction vessel transfer
The reaction vessel is held in the light source reflected light from reaction container bottom and incided on the position of the test section by mechanism,
And the reaction vessel is stopped certain time on the position,
The control unit implements the measure in the step of second light quantity checks.
10. automatic analysing apparatus as claimed in claim 4, it is characterised in that
Using come self-configuring it is described measure port top reflecting plate light source reflected light,
The control unit implements the measure in the step of second light quantity checks.
11. automatic analysing apparatus as claimed in claim 10, it is characterised in that
The reflecting plate has the function of the exterior light shadow shield of measure concurrently.
12. automatic analysing apparatus as claimed in claim 4, it is characterised in that
The measure port possesses the leaf spring of the fixed reaction vessel, and the leaf spring possesses reflecting plate,
When reaction vessel is not set to the measure port, the reflecting plate be positioned to make the light from light source towards
The position of the test section reflection,
The control unit implements the measure in the step of second light quantity checks.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014139275 | 2014-07-07 | ||
| JP2014-139275 | 2014-07-07 | ||
| PCT/JP2015/065712 WO2016006362A1 (en) | 2014-07-07 | 2015-06-01 | Automatic analysis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106471356A CN106471356A (en) | 2017-03-01 |
| CN106471356B true CN106471356B (en) | 2018-01-02 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580033562.9A Active CN106471356B (en) | 2014-07-07 | 2015-06-01 | Automatic analysing apparatus |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP6208356B2 (en) |
| CN (1) | CN106471356B (en) |
| WO (1) | WO2016006362A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109690320B (en) * | 2016-09-14 | 2022-11-08 | 株式会社日立高新技术 | Automatic analyzer and analysis method thereof |
| JP6951870B2 (en) * | 2017-06-02 | 2021-10-20 | 浜松ホトニクス株式会社 | Optical measuring device |
| JP6785989B2 (en) * | 2017-10-10 | 2020-11-18 | 株式会社日立ハイテク | Automatic analyzer |
| JP7241209B2 (en) * | 2020-01-17 | 2023-03-16 | 株式会社エイアンドティー | Blood Coagulation Measuring Device, Method for Measuring Blood Coagulation Time, Method for Determining Completion of Blood Coagulation Reaction, and Automated Blood Centrifuge |
| TWI765287B (en) * | 2020-06-23 | 2022-05-21 | 新加坡商克雷多診斷生物醫學私人有限公司 | Sample tube for polymerase chain reaction and polymerase chain reaction device thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57208439A (en) * | 1981-06-18 | 1982-12-21 | Olympus Optical Co Ltd | Particle coagulation discriminating vessel |
| JPS5928642A (en) * | 1982-08-11 | 1984-02-15 | Hitachi Ltd | Photometer for scattered light |
| JPS60154858U (en) * | 1984-03-26 | 1985-10-15 | 東亜医用電子株式会社 | sample container |
| JP3324560B2 (en) * | 1999-05-10 | 2002-09-17 | 株式会社島津製作所 | Blood coagulation analyzer |
| US20040258563A1 (en) * | 2003-06-23 | 2004-12-23 | Applera Corporation | Caps for sample wells and microcards for biological materials |
| US9207163B2 (en) * | 2007-04-20 | 2015-12-08 | Wako Pure Chemical Industries, Ltd. | Glass-cell vial for examining slight amount of specimen |
| JP5481402B2 (en) * | 2011-01-17 | 2014-04-23 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP5953129B2 (en) * | 2012-06-04 | 2016-07-20 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP5993737B2 (en) * | 2012-12-17 | 2016-09-14 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP5946776B2 (en) * | 2013-01-18 | 2016-07-06 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
-
2015
- 2015-06-01 CN CN201580033562.9A patent/CN106471356B/en active Active
- 2015-06-01 WO PCT/JP2015/065712 patent/WO2016006362A1/en active Application Filing
- 2015-06-01 JP JP2016532496A patent/JP6208356B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016006362A1 (en) | 2016-01-14 |
| CN106471356A (en) | 2017-03-01 |
| JP6208356B2 (en) | 2017-10-04 |
| JPWO2016006362A1 (en) | 2017-04-27 |
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