CN106471121A8 - 减少功能蛋白的水平基因转移 - Google Patents

减少功能蛋白的水平基因转移 Download PDF

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Publication number
CN106471121A8
CN106471121A8 CN201580022539.XA CN201580022539A CN106471121A8 CN 106471121 A8 CN106471121 A8 CN 106471121A8 CN 201580022539 A CN201580022539 A CN 201580022539A CN 106471121 A8 CN106471121 A8 CN 106471121A8
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Prior art keywords
gene transfer
functional protein
horizontal gene
reduce
protein
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CN201580022539.XA
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English (en)
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CN106471121A (zh
Inventor
E.E.布雷夫诺瓦
E.H.格林哈根
A.J.肖四世
C.R.索思
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Novogy Inc
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Novogy Inc
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Publication of CN106471121A publication Critical patent/CN106471121A/zh
Publication of CN106471121A8 publication Critical patent/CN106471121A8/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

公开的是用于减少功能蛋白的水平基因转移的方法与组合物。在一些实施方案中，通过在至少两种空间上不同的核酸序列上单独编码蛋白质的结构域来降低功能蛋白的水平基因转移的风险，其中各个体结构域单独为非功能性的，但是编码的结构域的共表达导致它们缔合以形成功能蛋白。
CN201580022539.XA 2014-03-03 2015-03-03 减少功能蛋白的水平基因转移 Pending CN106471121A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461947051P 2014-03-03 2014-03-03
US61/947,051 2014-03-03
PCT/US2015/018410 WO2015134440A1 (en) 2014-03-03 2015-03-03 Reducing horizontal gene transfer of functional proteins

Publications (2)

Publication Number Publication Date
CN106471121A CN106471121A (zh) 2017-03-01
CN106471121A8 true CN106471121A8 (zh) 2017-06-30

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ID=54055776

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CN201580022539.XA Pending CN106471121A (zh) 2014-03-03 2015-03-03 减少功能蛋白的水平基因转移

Country Status (5)

Country Link
US (1) US20170204401A1 (zh)
EP (1) EP3114226A4 (zh)
CN (1) CN106471121A (zh)
AU (1) AU2015224552A1 (zh)
WO (1) WO2015134440A1 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
EP4302824A3 (en) 2016-04-20 2024-03-20 The Board of Trustees of the Leland Stanford Junior University Compositions and methods for nucleic acid expression and protein secretion in bacteroides
US11566238B2 (en) 2016-12-15 2023-01-31 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for modulating growth of a genetically modified gut bacterial cell
EP3881078A1 (en) * 2018-11-15 2021-09-22 Quantum-Si Incorporated Methods and compositions for protein sequencing
EP4255455A1 (en) * 2020-12-01 2023-10-11 President And Fellows Of Harvard College Engineered probiotic compositions and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US7811803B2 (en) * 1997-06-19 2010-10-12 University Of Medicine And Dentistry Of New Jersey Methods and compositions for rapid purification of proteasomes and methods of use of components thereof
AU2001255797A1 (en) * 2000-05-02 2001-11-12 Applied Phytologics, Inc. Enhanced gene expression in plants using transcription factors
EP3467117B1 (en) * 2001-09-20 2020-07-15 Immunex Corporation Selection of cells expressing heteromeric polypeptides
US20070254338A1 (en) * 2006-04-24 2007-11-01 Amgen Inc. Method for making recombinant protein using complementation dependent DHFR mutants
WO2012009704A2 (en) * 2010-07-16 2012-01-19 Avantgen, Inc. Novel peptides and uses thereof
JPWO2014024998A1 (ja) * 2012-08-09 2016-07-25 国立大学法人広島大学 亜リン酸デヒドロゲナーゼ遺伝子をマーカーとした微生物の選択的培養方法

Also Published As

Publication number Publication date
US20170204401A1 (en) 2017-07-20
WO2015134440A1 (en) 2015-09-11
CN106471121A (zh) 2017-03-01
EP3114226A1 (en) 2017-01-11
AU2015224552A1 (en) 2016-09-15
EP3114226A4 (en) 2017-07-26

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PB01 Publication
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C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
CI01 Publication of corrected invention patent application

Correction item: Priority

Correct: 61/947,051 2014.03.03 US

Number: 09

Volume: 33

CI01 Publication of corrected invention patent application
CI02 Correction of invention patent application

Correction item: Priority

Correct: 61/947,051 2014.03.03 US

Number: 09

Page: The title page

Volume: 33

CI02 Correction of invention patent application
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20170301

WD01 Invention patent application deemed withdrawn after publication