CN106467294B - Inhibit the aqueous phase preparation method of tumour cell nanometer cadmium telluride - Google Patents
Inhibit the aqueous phase preparation method of tumour cell nanometer cadmium telluride Download PDFInfo
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- CN106467294B CN106467294B CN201610903022.8A CN201610903022A CN106467294B CN 106467294 B CN106467294 B CN 106467294B CN 201610903022 A CN201610903022 A CN 201610903022A CN 106467294 B CN106467294 B CN 106467294B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
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- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B19/00—Selenium; Tellurium; Compounds thereof
- C01B19/007—Tellurides or selenides of metals
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- C01P2002/70—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data
- C01P2002/72—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data by d-values or two theta-values, e.g. as X-ray diagram
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- C01P2004/00—Particle morphology
- C01P2004/01—Particle morphology depicted by an image
- C01P2004/04—Particle morphology depicted by an image obtained by TEM, STEM, STM or AFM
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- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/60—Particles characterised by their size
- C01P2004/64—Nanometer sized, i.e. from 1-100 nanometer
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Abstract
The present invention provides a kind of aqueous phase preparation method inhibiting tumour cell nanometer cadmium telluride, and the method is prepared using water phase, and with Sodium Hyaluronate, cadmium ion, tellurium ion is raw material；Cadmium ion is reacted with Sodium Hyaluronate first, is nucleated to nano material by Sodium Hyaluronate and growth regulates and controls；Then tellurium ion is added to be reacted；Unreacted ion is removed by molecular sieve after reaction, obtains the nanometer cadmium telluride that can be stable in the presence of in aqueous solution.The present invention is first acted on cadmium particle by the coordination of Sodium Hyaluronate, the nucleation of cadmium telluride and generation are limited in by it in certain space, to obtain the aqueous phase preparation method of nanometer cadmium telluride of the size less than 10 nanometers in aqueous solution, obtain that there is small size nanometer cadmium telluride under extremely mild reaction condition.
Description
Technical field
The invention belongs to the field of chemical synthesis, more particularly to inhibit the aqueous phase preparation method of tumour cell nanometer cadmium telluride.
Background technology
With the development of society, the transition of people's living environment and eating habit etc., have become people in modern cancer
One of the number one killer of class health.Up to the present, the treatment of cancer is still the severe challenge that modern medicine faces.Therefore,
It is horizontal that treatment of cancer is continuously improved, develops novel cancer means, develop more effective, the smaller anticancer drug of toxic side effect at
For the very urgent responsibility of vast related scientific research, medical personnel.In recent years, nano material and nanotechnology high speed development, and
It is widely used in multiple fields, new opportunity is provided to establish effective cancer diagnosis and treatment technology.
Cadmium telluride quantum dot has certain effect, so as cancer target macromolecule in lesion detection and oncotherapy
And current cadmium telluride quantum dot reaction requires high, prepares that raw material bio-compatibility is bad, and particle size is larger, limits telluride
The application of cadmium.
Invention content
The present invention provides a kind of tumour cell nanometer cadmium telluride that inhibits to overcome at least one deficiency of the prior art
Aqueous phase preparation method, particle size extra small purpose mild convenient for reaction condition to realize.
To achieve the goals above, the present invention uses following technical scheme：
A kind of aqueous phase preparation method inhibiting tumour cell nanometer cadmium telluride, the method is prepared using water phase, with transparent
Matter acid sodium, cadmium ion, tellurium ion are raw material；Cadmium ion is reacted with Sodium Hyaluronate first, by Sodium Hyaluronate to nanometer material
Material nucleation and growth are regulated and controled；Then tellurium ion is added to be reacted；Unreacted ion is removed by molecular sieve after reaction,
Obtain the nanometer cadmium telluride that can be stable in the presence of in aqueous solution.
Further, the cadmium ion is divalent cadmium ion.
Further, the cadmium ion is caddy or cadmium nitrate.
Further, the tellurium ion is divalent tellurium ion.
Further, reactant is a concentration of：The mass fraction of Sodium Hyaluronate is 1%~5%, and cadmium ion is 0.1~10
MM/l, tellurium ion is 0.1~10 mM/l；The total volume of reaction solution is 100 milliliters~100 liters.
Further, reaction temperature is 50-90 degree, and pressure is normal pressure.
Further, when reaction, cadmium ion is added dropwise in the sodium hyaluronate solution of agitation dropwise, and reaction overnight, makes the two
Fully chelating, obtains reaction solution 1；Then it is 60~500 micro- liters/min tellurium ion to be pumped control speed by miniflow, is slowly dripped
It adds in the reaction solution 1 quickly stirred；After being added dropwise, reaction is warming up to 60 degree, carries out 3 hours, obtains reaction solution 2；
Then it will be filtered sub-sieve in 2 molecular sieve of reaction solution, obtains final product.
Further, nanometer cadmium telluride obtained by the reaction obtains dry black solid powder by freeze-drying.
Further, nanometer cadmium telluride size obtained by the reaction is less than 10 nanometers.
Further, nanometer cadmium telluride obtained by the reaction can be scattered in the aqueous phase solutions such as distilled water or phosphate-buffered salt again
In, in aqueous solution at uniform, clear solution.
Compared with prior art, the present invention has the following advantages：
The present invention uses the preferable hyaluronic acid of biocompatibility for template, and material based on cadmium telluride passes through hyalomitome
The coordination of sour sodium is first acted on cadmium particle, and the nucleation of cadmium telluride and generation are limited in by it in certain space, from
And the aqueous phase preparation method of nanometer cadmium telluride of the size less than 10 nanometers has been obtained in aqueous solution, in extremely mild reaction condition
Under obtained that there is small size nanometer cadmium telluride, this quantum dot has wide in nanometer medicine and nanometer detection and imaging side face
Application prospect.
Specifically, advantages of the present invention further includes following several respects：
1. reaction condition is mild, green, energy saving, it can be used for volume production nanometer cadmium telluride；
2. being not necessarily to special experimental provision, operating procedure is simple；
3. product has extra small grain size；
4. final product has dispersibility well in distilled water and phosphate buffer；
5. product has obvious inhibiting effect to tumour cell, it is expected to be used for nanometer medicine aspect；
For above and other objects of the present invention, feature and advantage can be clearer and more comprehensible, preferred embodiment cited below particularly,
And coordinate attached drawing, it is described in detail below.
Description of the drawings
Fig. 1 is the nanometer cadmium telluride transmission electron microscope photo (TEM) that the embodiment of the present invention 1 synthesizes, and scale is in figure
100 nanometers, lateral size of dots is 3.3+/- 1.31 nanometers.
Fig. 2 is X-ray powder diffraction (XRD) the collection of illustrative plates for the nanometer cadmium telluride that the embodiment of the present invention 1 synthesizes
Fig. 3 is the nanometer cadmium telluride of the synthesis of the embodiment of the present invention 1 to cervical cancer cell Toxicity test figure.
Fig. 4 is the nanometer cadmium telluride of the synthesis of the embodiment of the present invention 1 to human liver cancer cell cytotoxicity investigation figure.
Specific implementation mode
Embodiment 1
Inhibit the aqueous phase preparation method of tumour cell nanometer cadmium telluride, specific preparation process as follows：
1. configuring 1% sodium hyaluronate solution 50ml with distilled water；All reaction process operate under nitrogen protection.
2. divalent cadmium ion (1mmol, 25ml) is added dropwise to the sodium hyaluronate solution that agitation speed is 30 revs/min dropwise
In, normal-temperature reaction is overnight, obtains reaction solution 1；
3. divalent tellurium ion (1mmol, 25ml) is placed in the syringe on syringe pump, and is added dropwise with the 100 μ L/ speed divided
To the reaction solution 1 that mixing speed is 60 revs/min, after being added dropwise, reaction is warming up to 60 degree, carries out 3 hours, is reacted
Solution 2；
4. 50mL reaction solutions 2 were impregnated as ultra-pure water molecular sieve super filter tube (MWCO (molecular cut off)=
100KDs), it is centrifuged for 2000 revs/min, is centrifuged 10 minutes, to remove the ion for not participating in reaction；
5. after centrifugations, 50mL distillation water washings being added and centrifuge products therefrom, 2000 revs/min are centrifuged, centrifugation
10 minutes, obtain reaction mixture；
6. final reacting product obtains dry black solid powder by freeze-drying (- 70 degrees Celsius)；
7. can be again scattered in distilled water or phosphate-buffered salt using preceding powder product.
8. performance test：The nanometer cadmium telluride that embodiment 1 specifically obtains has been subjected to pattern and ingredient determines characterization, specifically
Characterization method has transmission electron microscope, X-ray powder diffraction.
Fig. 1 is the nanometer cadmium telluride transmission electron microscope photo (TEM) that the embodiment of the present invention 1 synthesizes, and scale is in figure
100 nanometers, lateral size of dots is 3.3+/- 1.31 nanometers.Fig. 2 is the X-ray for the nanometer cadmium telluride that the embodiment of the present invention 1 synthesizes
Powder diffraction (XRD) collection of illustrative plates.It can prove that the product that embodiment 1 obtains is diameter receiving less than 10 nanometers from Fig. 1 and Fig. 2
Rice cadmium telluride.
Fig. 3 is the nanometer cadmium telluride of the synthesis of the embodiment of the present invention 1 to cervical cancer cell Toxicity test figure.Fig. 4 is this hair
The nanometer cadmium telluride that bright embodiment 1 synthesizes is schemed to being investigated to human liver cancer cell cytotoxicity.Wherein nanometer cadmium telluride is to people's cancer
The investigation method of cell cytotoxicity uses the investigation method-MTT colorimetric methods of this field routine, from figs. 3 and 4 it can be seen that
The nanometer cadmium telluride that the embodiment of the present invention 1 synthesizes all has certain cytotoxicity to cervical cancer cell and liver cancer cells.
Although the present invention is disclosed above by preferred embodiment, however, it is not intended to limit the invention, this any known skill
Skill person can make some changes and embellishment without departing from the spirit and scope of the present invention, therefore protection scope of the present invention is worked as
Subject to claims range claimed.
Claims (9)
1. a kind of aqueous phase preparation method inhibiting tumour cell nanometer cadmium telluride, which is characterized in that the method uses water phase system
Standby, with Sodium Hyaluronate, cadmium ion, tellurium ion is raw material；Cadmium ion is reacted with Sodium Hyaluronate first, passes through Sodium Hyaluronate
Nano material nucleation and growth are regulated and controled；Then tellurium ion is added to be reacted；It is not anti-by molecular sieve removing after reaction
The ion answered obtains the nanometer cadmium telluride that can be stable in the presence of in aqueous solution；When reaction, cadmium ion is added dropwise to agitation dropwise
In sodium hyaluronate solution, reaction overnight, makes the two fully chelating, obtains reaction solution 1；Then tellurium ion is pumped by miniflow
It is 60~500 micro- liters/min to control speed, is slowly added dropwise into the reaction solution 1 quickly stirred；After being added dropwise, reaction heating
To 60 degree, carries out 3 hours, obtain reaction solution 2；Then it will be filtered sub-sieve in 2 molecular sieve of reaction solution, is finally produced
Object.
2. the aqueous phase preparation method according to claim 1 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that described
Cadmium ion is divalent cadmium ion.
3. the aqueous phase preparation method according to claim 2 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that described
Cadmium ion is caddy or cadmium nitrate.
4. the aqueous phase preparation method according to claim 1 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that described
Tellurium ion is divalent tellurium ion.
5. the aqueous phase preparation method according to claim 1 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that reaction
Object it is a concentration of：The mass fraction of Sodium Hyaluronate is 1%~5%, and cadmium ion is 0.1~10 mM/l, and tellurium ion is
0.1~10 mM/l；The total volume of reaction solution is 100 milliliters~100 liters.
6. the aqueous phase preparation method according to claim 1 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that reaction
Temperature is 50-90 degree, and pressure is normal pressure.
7. the aqueous phase preparation method according to claim 1 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that reaction
Obtained nanometer cadmium telluride obtains dry black solid powder by freeze-drying.
8. the aqueous phase preparation method according to claim 1 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that reaction
Obtained nanometer cadmium telluride size is less than 10 nanometers.
9. the aqueous phase preparation method according to claim 1 for inhibiting tumour cell nanometer cadmium telluride, which is characterized in that reaction
Obtained nanometer cadmium telluride can be again scattered in the aqueous phase solutions such as distilled water or phosphate-buffered salt, in aqueous solution at it is uniform,
Clear solution.
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Citations (5)
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|---|---|---|---|---|
| CN1710154A (en) * | 2005-04-22 | 2005-12-21 | 吉林大学 | Method for water-phase rapid synthesis of CdTe nano crystal at warm condition |
| CN102408101A (en) * | 2010-09-21 | 2012-04-11 | 海洋王照明科技股份有限公司 | Preparation method of cadmium telluride quantum dot |
| CN103539082A (en) * | 2013-10-25 | 2014-01-29 | 湖南科技大学 | Quick green preparation method of cadmium telluride quantum dot |
| CN104495764A (en) * | 2015-01-19 | 2015-04-08 | 中国科学院长春应用化学研究所 | Cadmium telluride quantum dot and preparation method thereof |
| CN104844839A (en) * | 2015-03-23 | 2015-08-19 | 济南大学 | Preparation method of magnetic fluorescent composite nanoparticle |
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2016
- 2016-10-17 CN CN201610903022.8A patent/CN106467294B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1710154A (en) * | 2005-04-22 | 2005-12-21 | 吉林大学 | Method for water-phase rapid synthesis of CdTe nano crystal at warm condition |
| CN102408101A (en) * | 2010-09-21 | 2012-04-11 | 海洋王照明科技股份有限公司 | Preparation method of cadmium telluride quantum dot |
| CN103539082A (en) * | 2013-10-25 | 2014-01-29 | 湖南科技大学 | Quick green preparation method of cadmium telluride quantum dot |
| CN104495764A (en) * | 2015-01-19 | 2015-04-08 | 中国科学院长春应用化学研究所 | Cadmium telluride quantum dot and preparation method thereof |
| CN104844839A (en) * | 2015-03-23 | 2015-08-19 | 济南大学 | Preparation method of magnetic fluorescent composite nanoparticle |
Non-Patent Citations (1)
| Title |
|---|
| CdS and ZnS quantum dots embedded in hyaluronic acid films;G. Khachatryan;《Journal of Alloys and Compounds》;20090314;第403页2.1. Generation of quantum dots以及第405页4. Conclusions * |
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Effective date of registration: 20200708 Address after: Building 2, No. 189 Guangming Road, Zhuangshi street, Zhenhai District, Ningbo City, Zhejiang Province, 315000 Patentee after: Chuanming (Ningbo) Chemical Technology Co., Ltd Address before: Hangzhou City, Zhejiang province Binjiang District 310000 shore road 688 Building 5 room 1512 Patentee before: ZHEJIANG BIOHARMONIOUS TECHNOLOGY Co.,Ltd. |