CN106443020A - Test paper for detecting phenyl ethylamine as well as preparing method and method of application thereof - Google Patents
Test paper for detecting phenyl ethylamine as well as preparing method and method of application thereof Download PDFInfo
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- CN106443020A CN106443020A CN201610814386.9A CN201610814386A CN106443020A CN 106443020 A CN106443020 A CN 106443020A CN 201610814386 A CN201610814386 A CN 201610814386A CN 106443020 A CN106443020 A CN 106443020A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/575—Hormones
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Abstract
The invention discloses a test paper for detecting phenyl ethylamine. The test paper comprises a plastic film envelope, wherein the plastic film envelope has an opened end; a filter paper strip, wherein, the filter paper strip is accommodated in the plastic film envelope, one end of the filter paper strip is attached with a color developing agent layer, and the other end of the filter paper strip is close to the opened end. The invention further provides a preparing method of the test paper for detecting the phenyl ethylamine. The preparing method comprises the steps of preparing a color developing agent; soaking the test paper in the color developing agent, cutting the test paper into a strip after drying to obtain a filter paper strip with one end attached with the color developing agent layer; conducting plastic treatment on the filter paper strip to obtain the test paper for detecting the phenyl ethylamine. The invention further discloses a method of application for detecting the phenyl ethylamine. The method of the application comprises the steps of placing the opened end of the test paper for detecting the phenyl ethylamine into urine, and observing color changes of the test paper for detecting the phenyl ethylamine after taking out after still standing. The paper test for detecting the phenyl ethylamine can quickly detects the phenyl ethylamine in the urine, and has the advantages of simple structure, convenient use, easy operation and a low cost.
Description
Technical field
The present invention relates in urine love hormone detection technique field, it is more particularly related to a kind of quick
The reagent paper of love hormone and the preparation method of this reagent paper and using method in detection urine.
Background technology
Love (Love) is interpersonal strong attachment, gets close to, yearns for, and unselfish single-minded and no not to the utmost
The emotion of its heart.Pursuing burning hot love and sincere emotion is the main target that people lives.From biochemical angle, love
Generation be the appearance of this specific people, sound, abnormal smells from the patient, behavior etc., stimulate the brain of people, secreted corresponding chemicals
Matter.These materials make both sides produce " incoming call " sensation, that is, romantic, happy, happy, easily feel, define morning
Phase strong impulsion, i.e. love.
These love hormones are the material bases that love produces, maintains, and people can be allowed to be passionately in love.Love hormone present in human body
Mainly there are phenylethylamine (Phenylethylamine), dopamine (Dopamine) and norepinephrine
(norepinephrine).
Present analysis detect that the method for love hormone mainly passes through high performance liquid chromatography (HPLC-UV) and high-efficient liquid phase color
Spectrum-tandem mass spectrum (LC-MS/MS).The defect of said method is to be required for by complicated extraction step and expensive large-scale essence
Close instrument, needs special scientific and technical personnel's operation, is not suitable for large-scale promotion application.
Content of the invention
It is an object of the invention to solution at least the above, and provide the advantage that at least will be described later.
It is a still further object of the present invention to provide a kind of easy to use, cheap, and can quickly detect urine
The reagent paper of middle love hormone, this reagent paper is to detect love hormone in urine by chromogenic reaction method.
It is a still further object of the present invention to provide in a kind of detection urine the reagent paper of love hormone preparation method, its system
Standby process is simple.
It is a still further object of the present invention to provide in a kind of urine using detection love hormone reagent paper quick detection urine
The method of love hormone in liquid.
To achieve these goals, the invention provides a kind of detection love hormone reagent paper, including：
Plastic film envelopes, it has opening；
Filter paper bar, it is contained in described plastic film envelopes, and one end of described filter paper bar is attached with and love hormone knot
The developer layer that after conjunction, color changes, and one end of described filter paper bar is near described opening.
Preferably, the reagent paper of described detection love hormone, described filter paper bar is nitrocellulose filter paper bar, and described
The width of filter paper bar is 2-4cm, and length is 10-15cm, and thickness is 0.4-0.6mm.
Preferably, the reagent paper of described detection love hormone, in described display oxidant layer, interval is marked with the first graduation mark
With the second graduation mark.The distance between first graduation mark and the second graduation mark, for example, 3～8cm are arranged according to practical situation；With
And first graduation mark and described filter paper bar the distance between the edge of one end, for example, 1～3cm.
Present invention also offers a kind of preparation method of the reagent paper of detection love hormone, comprise the following steps：
Step one, ortho-nitrophenyl amine aqueous solution, sodium nitrite solution and solution of potassium carbonate are carried out according to certain volume ratio
Mix, obtain developer；
Step 2, one end of filter paper is immersed in described developer, standing is taken out after a period of time and dried, after drying
Filter paper be cut into the strip of certain length-width ratio, obtain the filter paper bar that one end is attached with developer layer；
Step 3, with plastic sheeting, described filter paper bar was carried out moulding process, obtained the detection love hormone of one end open
Reagent paper, and described filter paper bar is attached with one end of developer layer near described opening.
Preferably, in the described preparation method of reagent paper of detection love hormone, in described step one, ortho-nitraniline
Solution, sodium nitrite solution and solution of potassium carbonate are according to 1:1:1 volume ratio is mixed.
Preferably, in the described preparation method of reagent paper of detection love hormone, described time of repose is 1-2min.
Preferably, in the described preparation method of reagent paper of detection love hormone, also include to institute in described step 2
State developer layer and carry out marking process, obtain the first graduation mark and the second graduation mark.
Preferably, the dry process in the described preparation method of reagent paper of detection love hormone, in described step 2
Be will soak after filter paper be placed in fume hood, carry out normal temperature drying.
Preferably, in the described preparation method of reagent paper of detection love hormone, in described step one, described adjacent nitro
Aniline solution is 10% ortho-nitrophenyl amine aqueous solution；
Described sodium nitrite solution is 10% sodium nitrite solution；
Described potassium bicarbonate solution is 0.2% potassium bicarbonate solution.
Present invention also offers a kind of using method of the reagent paper of detection love hormone, including：
The opening of the reagent paper of detection love hormone is placed in the cup filling urine, and the liquid level of urine is higher than
First graduation mark, less than the second graduation mark, after standing 30s, takes out the reagent paper of described detection love hormone；
The reagent paper of the detection love hormone after taking out is placed 7-10min in the cool, observes the examination of detection love hormone
The color change of paper；
Wherein, if the color from light yellow of the developer layer of reagent paper of detection love hormone is changed into lilac, illustrate to urinate
Love hormone is contained in liquid；If the color of described developer layer is always maintained at faint yellow, illustrates not contain love in urine and swash
Element.
The present invention at least includes following beneficial effect：
1st, to have structure simple, easy to use, with low cost, practical for the reagent paper of detection love hormone of the present invention
Advantage is it is easy to operation is it is not necessary to special scientific and technical personnel operate, and can quickly detect the love hormone in urine, and examines
Survey sensitive.
2nd, the preparation process of the reagent paper of detection love hormone of the present invention is simple, greatly reduces production cost and inspection
Survey cost, suitable large-scale promotion application.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by this
Invention research and practice and be understood by the person skilled in the art.
Brief description
Fig. 1 is the structural representation of the reagent paper of detection love hormone described in the one of embodiment of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is described in further detail, with make those skilled in the art join
Book word can be implemented according to this as directed.
It should be appreciated that used herein such as " have ", "comprising" and " inclusion " term are not precluded from one or many
The presence of individual other element or a combination thereof or interpolation.
As shown in figure 1, the invention provides a kind of detection love hormone reagent paper, including：
Plastic film envelopes, it has opening；
Filter paper bar, it is contained in described plastic film envelopes, and one end of described filter paper bar is attached with and love hormone knot
The developer layer 3 that after conjunction, color changes, in described display oxidant layer, interval is marked with the first graduation mark 1 and the second graduation mark 2,
And one end of described filter paper bar is near described opening.
Wherein, described filter paper bar is nitrocellulose filter paper bar, and the width of described filter paper bar is 2-4cm, and length is 10-
15cm, thickness is 0.4-0.6mm.
The preparation method of the reagent paper of above-mentioned detection love hormone is specially：
Step one, 10% ortho-nitrophenyl amine aqueous solution, 10% sodium nitrite solution and 0.2% solution of potassium carbonate are pressed
According to 1:1:1 volume ratio mix homogeneously, obtains developer；
Step 2, one end of filter paper is immersed in described developer, takes out after standing 1min, normal temperature drying 4 hours with
On, by its cutting into strips (width 2-4cm, length is 10-15cm), obtain the filter paper bar that one end is attached with developer layer；And
Marking process is carried out to described developer layer, obtains the first graduation mark and the second graduation mark.
Step 3, with plastic sheeting, described filter paper bar was carried out moulding process, obtained the detection love hormone of one end open
Reagent paper, and described filter paper bar is attached with one end of developer near described opening；And by the detection preparing love hormone
Reagent paper being dried, ventilation preserves, it is to avoid direct sunlight.
The using method of the reagent paper of above-mentioned detection love hormone is specially：
(1) Disposable paper cup of cleaning, the urine of collecting test paper, are taken, height in dixie cup for the urine is more than 1cm, urine
Liquid is preferably the first time urine of morning empty stomach；
(2), the opening of the reagent paper of detection love hormone is placed in the cup filling urine, and the liquid level of urine
Higher than the first graduation mark, less than the second graduation mark, after standing 30s, take out the reagent paper of described detection love hormone；
(3), the reagent paper of the detection love hormone after taking out is placed 7-10min in the cool, the color observing reagent paper becomes
Change；
Wherein, if the color from light yellow of the opening of reagent paper is changed into lilac, illustrate to contain love hormone in urine,
Prompting tester is in the sweet affection stage at present；If the color of the opening of reagent paper is always maintained at faint yellow, illustrate
Love hormone is not contained in urine.
Love hormone typically only when people are in sweet affection or strong when liking someone,
Just can produce in a large number, concentration in urine for the love hormone>Easily it is detected during 20 μ g/mL.When not having love to arrive,
These hormones are typically very low in urine content, are difficult to be detected.
In order to verify the accuracy using love hormone in the detection paper urine of the detection love hormone of present invention offer,
The present invention takes 100 big two, certain colleges and universities and the urine of junior to be detected at random, wherein boy student 60, schoolgirl 40.
Reagent paper using detection love hormone of the present invention judges whether it is being in love state, and with tester's oneself
Feel to be compared, result is as shown in table 1.
The effect that love assessed by table 1 love hormone reagent paper is compared with tester's readme
Judge the rate of accuracy reached 87.1% of love by the reagent paper that table 1 shows detection love hormone of the present invention, specifically
Property be 79.4%.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Realize other modification, therefore under the general concept being limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details.
Claims (10)
1. a kind of reagent paper of detection love hormone is it is characterised in that include：
Plastic film envelopes, it has opening；
Filter paper bar, it is contained in described plastic film envelopes, and one end of described filter paper bar is attached with after being combined with love hormone
The developer layer that color changes, and one end of described filter paper bar is near described opening.
2. the reagent paper of detection love hormone as claimed in claim 1 is it is characterised in that described filter paper bar is filtered for celluloid
Paper slip, and the width of described filter paper bar is 2-4cm, length is 10-15cm, and thickness is 0.4-0.6mm.
3. the reagent paper of detection love hormone as claimed in claim 1 is marked with it is characterised in that being spaced in described display oxidant layer
First graduation mark and the second graduation mark.
4. the reagent paper of detection love hormone as described in a kind of any one as claims 1 to 3 preparation method it is characterised in that
Comprise the following steps：
Step one, ortho-nitrophenyl amine aqueous solution, sodium nitrite solution and potassium bicarbonate solution are mixed according to certain volume ratio
Even, obtain developer；
Step 2, one end of filter paper is immersed in described developer, standing is taken out after a period of time and dried, by the filter after drying
Paper is cut into the strip of certain length-width ratio, obtains the filter paper bar that one end is attached with developer layer；
Step 3, with plastic sheeting, described filter paper bar was carried out moulding process, obtained the examination of the detection love hormone of one end open
Paper, and described filter paper bar is attached with one end of developer layer near described opening.
5. the preparation method of the reagent paper of detection love hormone as claimed in claim 4 is it is characterised in that in described step one,
Ortho-nitrophenyl amine aqueous solution, sodium nitrite solution and solution of potassium carbonate are according to 1:1:1 volume ratio is mixed.
6. the preparation method of the reagent paper of detection love hormone as claimed in claim 4 is it is characterised in that described time of repose is
1-2min.
7. the preparation method of the reagent paper of detection love hormone as claimed in claim 4 is it is characterised in that go back in described step 2
Carry out marking process including to described developer layer, obtain the first graduation mark and the second graduation mark.
8. the preparation method of the reagent paper of detection love hormone as claimed in claim 4 is it is characterised in that in described step 2
Dry process be will soak after filter paper be placed in fume hood, carry out normal temperature drying.
9. the preparation method of the reagent paper of detection love hormone as claimed in claim 4 is it is characterised in that in described step one,
Described ortho-nitrophenyl amine aqueous solution is 10% ortho-nitrophenyl amine aqueous solution；
Described sodium nitrite solution is 10% sodium nitrite solution；
Described potassium bicarbonate solution is 0.2% potassium bicarbonate solution.
10. the reagent paper of detection love hormone as described in a kind of any one as claim 1～9 using method it is characterised in that
Including：
The opening of the reagent paper of detection love hormone is placed in the cup filling urine, and the liquid level of urine is higher than first
Graduation mark, less than the second graduation mark, after standing 30s, takes out the reagent paper of described detection love hormone；
The reagent paper of the detection love hormone after taking out is placed 7-10min in the cool, observes the reagent paper of detection love hormone
Color change；
Wherein, if the color from light yellow of the developer layer of reagent paper of detection love hormone is changed into lilac, illustrate in urine
Containing love hormone；If the color of described developer layer is always maintained at faint yellow, illustrate in urine, not containing love hormone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610814386.9A CN106443020A (en) | 2016-09-09 | 2016-09-09 | Test paper for detecting phenyl ethylamine as well as preparing method and method of application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610814386.9A CN106443020A (en) | 2016-09-09 | 2016-09-09 | Test paper for detecting phenyl ethylamine as well as preparing method and method of application thereof |
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| Publication Number | Publication Date |
|---|---|
| CN106443020A true CN106443020A (en) | 2017-02-22 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610814386.9A Pending CN106443020A (en) | 2016-09-09 | 2016-09-09 | Test paper for detecting phenyl ethylamine as well as preparing method and method of application thereof |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4260393A (en) * | 1979-01-15 | 1981-04-07 | Technology Resources, Inc. | Method and combined test elements for detection of heme |
| CN1229920A (en) * | 1998-03-23 | 1999-09-29 | 陈冰铨 | Test paper for detecting cancer relative factor from urine |
| CN1615441A (en) * | 2002-01-09 | 2005-05-11 | 因韦尔尼斯医药瑞士股份有限公司 | Liquid sample assay device |
| CN1749751A (en) * | 2004-09-15 | 2006-03-22 | 王志洪 | Detecting test paper strip for measuring alcohol content in human saliva |
| WO2009143601A1 (en) * | 2008-05-27 | 2009-12-03 | Zbx Corporation | Enzymatic analytical membrane, test device and method |
| CN101900684A (en) * | 2009-10-16 | 2010-12-01 | 北京智云达科技有限公司 | Test paper for quickly detecting urea in liquid milk and preparation method thereof |
| CN104345058A (en) * | 2013-08-08 | 2015-02-11 | 上海索诚化学试剂有限公司 | Detection test paper |
-
2016
- 2016-09-09 CN CN201610814386.9A patent/CN106443020A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4260393A (en) * | 1979-01-15 | 1981-04-07 | Technology Resources, Inc. | Method and combined test elements for detection of heme |
| CN1229920A (en) * | 1998-03-23 | 1999-09-29 | 陈冰铨 | Test paper for detecting cancer relative factor from urine |
| CN1615441A (en) * | 2002-01-09 | 2005-05-11 | 因韦尔尼斯医药瑞士股份有限公司 | Liquid sample assay device |
| CN1749751A (en) * | 2004-09-15 | 2006-03-22 | 王志洪 | Detecting test paper strip for measuring alcohol content in human saliva |
| WO2009143601A1 (en) * | 2008-05-27 | 2009-12-03 | Zbx Corporation | Enzymatic analytical membrane, test device and method |
| CN101900684A (en) * | 2009-10-16 | 2010-12-01 | 北京智云达科技有限公司 | Test paper for quickly detecting urea in liquid milk and preparation method thereof |
| CN104345058A (en) * | 2013-08-08 | 2015-02-11 | 上海索诚化学试剂有限公司 | Detection test paper |
Non-Patent Citations (1)
| Title |
|---|
| 杜彩云，李忠义主编: "《有机化学》", 31 December 2015, 武汉：武汉大学出版社 * |
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Application publication date: 20170222 |