CN106442973A - Fungaltoxin multi-parameter quantitative detection kit in field of food security - Google Patents
Fungaltoxin multi-parameter quantitative detection kit in field of food security Download PDFInfo
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- CN106442973A CN106442973A CN201610809865.1A CN201610809865A CN106442973A CN 106442973 A CN106442973 A CN 106442973A CN 201610809865 A CN201610809865 A CN 201610809865A CN 106442973 A CN106442973 A CN 106442973A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
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Abstract
The invention provides a multi-parameter detection kit for quickly detecting fungaltoxin in the field of food security. Through combined application of different parameters in the detection of the fungaltoxin and a fungaltoxin detection card, the operation is simple and easy, the accurate content of different types of fungaltoxin in different samples, such as corn and by-products thereof, cereal, flour, bran, bean meal, cottonseed meal, peanut meal, corn germ meal, rapeseed meal, batch and concentrate, can be quickly and accurately determined, and the kit has an important significance to food security.
Description
Technical field
The invention provides the quick detection reagent of the multiple parameter method Accurate Determining mycotoxin levels of field of food safety
Box, the invention belongs to biological detection field of engineering technology.
Background technology
Mycotoxin (mycotoxin) a series of is tied with different chemistry by what some funguses were produced in growth course
The poisonous secondary metabolite of structure.The mycotoxin species being currently known has kind more than 300, wherein there are about more than 30 kind mycotoxins pair
Human and animal has strong toxicity, and some fungi toxin shows as induced gene mutation, produces carcinogenecity, and certain organs
Toxicity etc.；Main fungal toxin includes：Aflatoxin, ochratoxin, 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its derivatives class toxin, vomit
Tell toxin fumonisin etc..Mycotoxin can extensively contaminated food, crops and its product etc., easily cause eater to be poisoned, make
Become heavy economic lossess, according to FAO (Food and Agriculture Organization of the United Nation) (FAO) data, the whole world there are about 25% crops every year and be subjected to mycotoxin
Pollution, the crops that there are about 2% lose nutrition and economic worth because seriously polluted, and the direct and consequential damage for causing reaches number
10000000000 dollars.
Existing mycotoxin detection method mainly includes：Thin layer chromatography, precision instrument analytic process and immune analysis
Method etc..
During thin layer chromatography detection mycotoxin, detectable consumption is big, complex operation, component serious interference, accuracy
Difference, it is impossible to accurate quantitative analysis, larger to experimenter and ambient contamination harm, it is unsuitable for field quick detection, applies less.
Precision instrument analytic process mainly include chromatography, mass spectrography and tandem mass spectrum combination etc. method, advantage be sensitivity
Height, accuracy is good, has the disadvantage expensive equipment, and sample pretreatment process is loaded down with trivial details, the purity requirement of sample height, environment and testing staff
Have high demands, testing cost height, be not suitable for field quick detection and popularization.As patent, " 103713065 B of CN is a kind of while examining
The method for surveying multiple mycotoxins " is described, application Liquid Chromatography-Tandem Mass Spectrometry technology (LC-MS/MS), dilute by Isotopic Internal Standard
Multiple mycotoxins in interpretation of the law Accurate Determining sample, isotopic application equally has certain Health cost to testing staff.
In recent years, application of the immune analysis method in mycotoxin is determined is more and more extensive, is mostly derived from immune credit
Analysis method sensitive, special, stable, easy the advantages of.
Enzyme-linked immunoassay method (ELISA) in immune analysis method is needed to add toxin standard substance, increases in detection process
Add testing staff by the risk of endotoxin contamination, simultaneously need to standard curve is drawn, calculating process is more complicated, time-consuming longer, no
Suitable field quick detection.As patent " a kind of construction method of mycotoxin Test database of 104569407 A of CN, funguses poison
Plain detection method, test kit and device " is described, although standard curve, standard substance are improved to some extent, but still demand sets up a Pang
Big fungal detection data base, while the single standard substance of application are carried out.
Gold colloidal mark detection technique in immune analysis method, with naked eyes sentence read result, testing cost is low, analysis time
, also there are relatively broad application and patented technology application short the advantages of, but gold colloidal is detected due to naked eyes interpretation, is existed and is difficult to standard
Determination amount, sensitivity is low, detects single grade for shortcomings.
Further, since the complexity of sample, such as：Semen Maydiss and its by-product, corn, flour, wheat bran, bean cake, cottonseed meal, Semen arachidis hypogaeae
The dregs of rice, corn germ cake, rapeseed meal, batch, concentrate feed etc., the accurate measurement to multiple mycotoxins has larger interference
And uncertain, and for the accurately parameter card of measurement and the application of the mycotoxin in different complex samples, in existing method
And all no corresponding records in patented technology.
Therefore, it is necessary to provide a kind of can the parameter card of mycotoxin and bag in easy, quick, accurate quantitative analysis measuring samples
The mycotoxin detection kit of containing parameter card.
Content of the invention
In order to solve the deficiency of prior art problem, the invention provides a kind of multiple parameter method of field of food safety is accurate
The quick detection kit of mycotoxin levels and application is determined, the test kit includes the fast of parameter card and mycotoxin levels
Fast detectable, the method energy is accurate, quick, quantitative determine mycotoxin in sample, and can be by the ginseng of difference sample types
Number card is calculated automatically, accurately obtains the exact level of the mycotoxin in different samples.
The invention provides mycotoxin levels in a kind of dissimilar sample of the quantitative determination for field of food safety
Solid-phase immunity chromatographic assays detection kit, the test kit includes the inspection of parameter card and solid-phase immunity chromatographic assays
Test agent.
In a preferred experimental program of the present invention, in the parameter card, include the data of different sample types
Information, when quantitative determining, calculates the exact level of the mycotoxin in different sample types automatically for mycotoxin.
In a preferred experimental program of the present invention, the mycotoxin detection examination of the solid-phase immunity chromatographic assays
Agent, it is characterised in that the detectable of the solid-phase immunity chromatographic assays is prepared from based on immunochromatographiassays assays principle,
Specifically include get stuck, backboard, gripper shoe, the gripper shoe through mycotoxin antigen and corresponding antibody labeling and/or is coated, described
Gripper shoe is selected from nitrocellulose filter, glass, absorbent paper and its equivalent material.
In a preferred experimental program of the present invention, the parameter card is preferably carried with cassette, bar formula or chip type
For；Chip type includes built-in chip and two kinds of forms of chip card.
In a preferred experimental program of the present invention, the parameter card is direct by being adapted to fluorescence immunity analyzer
The exact level of the mycotoxin for calculating automatically in different sample types is independently operated, when mycotoxin is quantitative determined, need not
Using the corresponding sterling standard substance calibration of mycotoxin or correction, easy to operate, there is to operator protection well and make
With.
In a preferred experimental program of the present invention, the different sample types that the parameter card includes, including but
It is not limited to following major sample type：Wheat bran, popcorn, bean cake, cottonseed meal, Semen arachidis hypogaeae dregs, corn germ cake, rapeseed meal, cooperation
Material, concentrate feed, Semen Maydiss and its by-product, corn, flour etc..
In a preferred experimental program of the present invention, at least wrapping per a parameter card information in the parameter card
Contain, such as：Wheat bran, popcorn, bean cake, cottonseed meal, Semen arachidis hypogaeae dregs, corn germ cake, rapeseed meal, batch, concentrate feed, Semen Maydiss and its
At least one sample message of the difference sample such as by-product, corn, flour.
In a preferred experimental program of the present invention, the mycotoxin including but not limited to known more than 300 is planted
Mycotoxin and more than 30 is planted and has supervirulent mycotoxin to human and animal.
In a preferred experimental program of the present invention, the mycotoxin includes：Aflatoxin, Aspergillus ochraceus poison
Element, 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its derivatives class toxin, vomitoxin, at least one of fumonisin.
In a preferred experimental program of the present invention, the solid-phase immunity chromatographic assays on turn electrochemiluminescent immunoassay layer
Appointing in the solid-phase immunity chromatographic assays such as analysis method, fluorescence immune chromatography method, colloidal gold immunity chromatography, latex immunochromatographic method
A kind of.
In a preferred experimental program of the present invention, mycotoxin detection kit is used for field of food safety difference
The accurate quantitative analysis of the different types of mycotoxin in sample are determined.
Different types of mycotoxin in the preferred experimental program of the present invention, in the difference samples
Accurate quantitative analysis minute is less than 20 minutes.
Compared with prior art, benefit of the invention is that there is provided a kind of multiple parameter method Accurate Determining including parameter card
The quick detection kit of mycotoxin levels and application, the method energy is accurate, quick, quantitative determine mycotoxin in sample,
And can be calculated by the parameter card of different sample types automatically, accurately obtain the exact level of the mycotoxin in different samples；
By the application of multiparameter in the present invention, first to different samples, such as：Semen Maydiss and its by-product, corn, flour, wheat bran, bean
Multiple mycotoxins in the samples such as the dregs of rice, cottonseed meal, Semen arachidis hypogaeae dregs, corn germ cake, rapeseed meal, batch, concentrate feed accurate, fixed
Measure fixed, safe to food mycotoxin detection important in inhibiting.
Specific embodiment
Following case study on implementation, the present invention will be described in further detail, it will be appreciated that case study on implementation described herein is only
For the present invention is explained, invention scope is not limited.In addition to having specified otherwise, raw and auxiliary material used by the present invention, including funguses poison
Plain antigen and the antibody of corresponding antifungal Venom antigens, are all from maturation, commercialization raw and auxiliary material.
The invention provides a kind of quick detection of the multiple parameter method Accurate Determining mycotoxin levels of field of food safety
Test kit and application, are preferably to explain the present invention, and preferably above forwarding light immunochromatographic assays explain the present invention, with
Lower upper forwarding light immunochromatographic assays case study on implementation is only used for preferably explaining the present invention, not as the restriction bar of the present invention
Part.
Upper forwarding light immunochromatographic assays and fluorescence immune chromatography method, colloidal gold immunity chromatography, latex immunochromatography
Method etc. belongs to solid-phase immunity Chromatographic techniques, and its difference is solid phase labelling particulate matter difference, as above turns electrochemiluminescent immunoassay
The UCP granule of chromatography, the colloid gold particle of colloidal gold immunity chromatography, the latex particle of latex immunochromatographic method, identical
Under the conditions of supplementary material, approach described above possesses similar technical process and detecting step, but upper forwarding light immunochromatography technique
Sensitivity, specificity be higher than additive method, and can accurate quantitative analysis, therefore from upper forwarding light immunochromatographic assays reality
Apply case and preferably explain the present invention, but the restrictive condition not as the present invention.
Case study on implementation 1：
1st, the preparation based on the upper mycotoxin levels quick detection parameter card for forwarding light immunochromatographic method：
Preparation in the implementation case based on the upper mycotoxin levels quick detection parameter card for forwarding light immunochromatographic method
Method is only the preparation method for preferably explaining parameter card, the preparation not as parameter card and the limiting mode for using.
According to known sample, such as：Semen Maydiss and its by-product, corn, flour, wheat bran, bean cake, cottonseed meal, Semen arachidis hypogaeae dregs, Semen Maydiss
The sample types such as the plumule dregs of rice, rapeseed meal, batch, concentrate feed, characteristic etc. are analyzed and summarized, through the substantial amounts of proficiency of our company
Data is carried out to the response rate of different mycotoxin, sensitivity, specificity, homogeneity, suitability etc. in different samples point
Analysis, the parameter for obtaining parameter card calculates data, experimentation and analysis method be all if no special instructions using conventional ripening side
Method is carried out.By parameter composing software by parameter typing parameter card, parameter card can be prepared into and mycotoxin detectable
Appearance forrns identical detectable bar pattern, it is also possible to which implanted chip mycotoxin is detected by card with the pattern of built-in chip
Internal.
Based on the upper mycotoxin levels quick detection parameter card for forwarding light immunochromatographic method, according to above-mentioned analysis method,
According to different sample types, the different classifications form being mainly prepared into as table 1, aflatoxin selected by the mycotoxin
B1, for preferably explaining the present invention, not as restrictive condition of the present invention to mycotoxin.
Table 1：AFB1 quick detection parameter card classification chart
Case study on implementation 2：
In the implementation case there is provided a kind of field of food safety multiple parameter method Accurate Determining mycotoxin levels fast
The preparation method of fast detectable, the preparation method is only preferably explains the present invention, true not as multiple parameter method Accurate Determining
The preparation of the quick detection reagent of bacterium cellulose content and the restrictive condition for using.
If no special instructions, true based on the upper multiple parameter method Accurate Determining for forwarding light immunochromatography technique in the implementation case
The preparation method of the quick detection reagent of bacterium cellulose content derives from our company's patented technology, and " 1690711 B of CN is based on upper turn
Change luminescence technology immuno-chromatographic test paper strip " in preparation method and its optimization method.
In the implementation case there is provided a kind of field of food safety multiple parameter method Accurate Determining mycotoxin levels fast
The preparation of fast detectable, the mycotoxin is used for preferably explaining the present invention from AFB1, not as this
The bright restrictive condition to mycotoxin.
1st, technique is coated based on upper forwarding light immunochromatography technique：
This is coated technique and is only preferably the explanation present invention, the preparation not as capsulating material and the restrictive condition for using.
Table 2：AFB1 is coated Process ba- sis formula table
| Classification | Basic components |
| Detection line T (AFB1 antigen) | 1.0mg/mL |
| Nature controlling line C (sheep anti mouse) | 0.25mg/mL |
| T line is coated buffer | 0.05M PB(pH7.4)+150mM NaCl |
| C line is coated buffer | 0.01M PB(pH7.2) |
| Auxiliary material | NC film, absorbent paper, PVC board |
According to the formula in table 2, by the T line of detection line T respectively containing AFB1 antigen and the nature controlling line containing sheep anti mouse
It is coated liquid and C line is coated liquid and is drawn with full-automatic Film-cutting machine respectively and is being attached on celluloid in PVC board (NC) film, wherein PVC
Plate is played a supportive role in bottom, and has absorbent paper to paste in one end also note near nature controlling line above NC film.
Coated NC film is placed in clear production Factory Building of the room epidemic disaster less than or equal to 30% and is dried, when drying
Between be no less than 15h, the NC film for drying is put into plus the aluminium foil bag interior sealing of desiccant is saved backup.
2nd, based on upper forwarding light immunochromatography technique marking process：
The marking process is only preferably explains the present invention, the preparation not as marker material and the restrictive condition for using.
Table 3：The main supplementary material inventory of AFB1 marking process：
Labelling start before by 20%BSA, traget antibody, be positioned over room temperature balance.
Granule prepares：Take UCP aldehyde radical granule 50mg, add 1ml deionized water, ultrasonic 30s, pressure-vaccum makes UCP granule resuspended
And uniform state is reached, it is transferred in 50ml labelling bottle；Add 1ml deionized water, ultrasonic 10s, pressure-vaccum is uniformly transferred to three
Angle bottle；Mending into labelling bottle cumulative volume is added water to for 10ml；Rock ultrasound 120 seconds after mixing.
Marking process：In the state of ultrasound, take 50 μ l of AFB1 antibody and be added rapidly in labelling bottle, mix 10 minutes.
Closing process：After labelling mixing time terminates, in labelling bottle, 50 μ l 20%BSA are added to be closed, closing 5
Minute.
Centrifugation：After closing terminates, 4 DEG C of centrifugations, 30min is centrifuged with 12000rpm rotating speed, supernatant is discarded, retain precipitation.
Prepared by markers work pad：Take 1.0ml conjugate and liquid is preserved, add in above-mentioned precipitation, make UCP granule resuspended, obtain
The UCP AFB1 conjugate of concentration, adds 50ml conjugate lyophilizing liquid, mixes, then according to 80-120 μ l/cm2It is uniformly coated on
On the glass fibre of 20cm × 29cm size, being put in freeze dryer, rule of operation evacuation lyophilizing, lyophilizing is used with reference to freeze dryer
Time is no less than 10 hours.After evacuation terminates, conjugate pad is taken out from freeze dryer, be immediately placed in equipped with desiccant from
In envelope, then one layer of aluminium foil bag is covered, sealing preserve is standby.
3rd, the preparation based on the upper mycotoxin levels quick detection reagent for forwarding light immunochromatography technique
Markers work pad is cut into 0.5-1cm width in clear production Factory Building, careful pastes in coated NC
On film, position being pasted in corresponding one end of absorbent paper, is prepared into the big plate of detection, the aluminium foil bag being put into equipped with desiccant, sealing guarantor
Deposit standby.
To detect that big plate cuts into the width of 0.3-0.6cm according to the specification for getting stuck, load in getting stuck accordingly, buckle well card
Shell, the aluminium foil bag being put into getting stuck equipped with desiccant, sealing preserve, that is, complete the system of AFB1 quick detection reagent
Standby.
AFB1 quick detection reagent is assembled into finished product with AFB1 parameter card, obtains final product Aspergillus flavus poison
Plain B1 multiparameter immue quantitative detection reagent box (above turning luminescence method), test kit key component is：AFB1 detection card
(40T), 1 part of description, AFB1 sample diluting liquid (40mL/ bottle, totally 1 bottle), AFB1 parameter card (3,
Parameter card 1,2,3).
Case study on implementation 3：
The invention provides a kind of quick detection of the multiple parameter method Accurate Determining mycotoxin levels of field of food safety
The using method of reagent, be more preferable and explain the present invention, in the implementation case there is provided a kind of field of food safety many ginsengs
The using method of the quick detection reagent of number method Accurate Determining AFB1 content, the using method is mainly used in not same
The measure of the exact level of AFB1 in product, detection sample includes：Wheat bran, popcorn, bean cake, cottonseed meal, Semen arachidis hypogaeae dregs,
Corn germ cake, rapeseed meal, batch, concentrate feed, Semen Maydiss and its by-product, corn, flour, other raw-food materials etc..
The using method of the quick detection reagent of following AFB1 content is mainly used in preferably explaining the present invention
Use and apply, not as the restrictive condition of application of the present invention.
Predominantly detect step as follows：
1st, Sample pretreatment：
Representative sample is taken, is ground using grinder, enable at least 75% ground sample to pass through 20 mesh sieves
Net, is thoroughly mixed and to sample secondary sample.
3.0g ground sample is accurately weighed in the centrifuge tube of 50mL, adds 15mL sample extracting solution, with vortex instrument or
Shaking table fully shaking 5min.
After concussion terminates, the extracting solution of 1.0mL-1.5mL is pipetted to clean centrifuge tube, 4000rpm is centrifuged 2min, takes
100 μ L of supernatant liquid, are added in 300 μ L sample diluents, and mixing is standby, (if liquid is in cloudy state after mixing, Ke Yichong
Multiple centrifugation, after 4000rpm centrifugation 2min, takes supernatant and is detected).
2nd, detecting step
By upper forwarding light immunity analysis instrument start, 15min is preheated, according to sample type, inserts this batch products corresponding
And the parameter card consistent with sample type carries out school ginseng.
Open the packaging of aluminium foil bag of detection card, horizontal positioned.
100 μ L are drawn with pipettor to add in the well of detection card through the testing sample of pre-treatment, start timing
15min.
After 15min, " manual measurement " entrance " input ID interface " is clicked on, is surveyed according to interface information scanning after clicking on " determination "
Test card is inserted forwarding light immunity analysis instrument after scanning success and is measured by examination card bottom bar code.
Detection by quantitative result shows on the instrument screen, while papery examining report can be obtained by printing key.
Case study on implementation 4：
The invention provides a kind of quick detection of the multiple parameter method Accurate Determining mycotoxin levels of field of food safety
The using method of reagent, be more preferable and explain the present invention, in the implementation case there is provided a kind of field of food safety many ginsengs
The testing result and performance indications, the testing result and property of the quick detection reagent of number method Accurate Determining AFB1 content
Energy index is mainly used in the measure of the exact level of AFB1 in different samples, and detection sample includes：Wheat bran, expanded jade
Rice, bean cake, cottonseed meal, Semen arachidis hypogaeae dregs, corn germ cake, rapeseed meal, batch, concentrate feed, Semen Maydiss and its by-product, corn, flour,
Other etc..
The testing result and performance indications of the quick detection reagent of following AFB1 content is mainly used in preferably
Using and applying for the present invention is explained, not as the restrictive condition of application of the present invention.
Main performance index such as table 4：
Table 4：AFB1 multiparameter immue quantitative detection reagent box (above turning luminescence method) product performance index
Above-mentioned case study on implementation describes the case that is preferable to carry out of the present invention, as previously mentioned, it should be understood that to the present invention's
Explain, and the form of case study on implementation of the present invention disclosure is not limited to, should not regard the exclusion to other embodiment as, and can use
Combination, modification in various other forms, and can change within the spirit and scope of the present invention.What those skilled in the art were carried out changes
Dynamic and change, all should be in scope of the appended claims of the present invention in present inventive concept and scope.
Claims (7)
1. in a kind of dissimilar sample of quantitative determination for field of food safety, the solid-phase immunity of mycotoxin levels is chromatographed
The detection kit of analytic process, the test kit includes the mycotoxin detection examination of parameter card and solid-phase immunity chromatographic assays
Agent.
2. detection kit as claimed in claim 1, wherein includes the data of different sample types in the parameter card
Information, calculates the content of the mycotoxin in different sample types automatically for mycotoxin when quantitative determining.
3. the mycotoxin detection examination of detection kit as claimed in claim 1, wherein the solid-phase immunity chromatographic assays
Agent is prepared from based on immunochromatography principle, its specifically include get stuck, backboard, nitrocellulose filter, glass fibre membrane, water suction
Paper；Wherein the nitrocellulose filter is coated through mycotoxin antigen and corresponding antibody, and the glass fibre membrane distribution is by through true
Verticillium toxin corresponds to the tracer grain of antibody labeling.
4. detection kit as claimed in claim 1 or 2, the wherein parameter card are direct by being adapted to fluorescence immunity analyzer
The content of the mycotoxin for calculating automatically in different sample types is independently operated, when mycotoxin is quantitative determined.
5. detection kit as claimed in claim 1 or 2, wherein the difference sample type are selected from wheat bran, popcorn, bean
The dregs of rice, cottonseed meal, Semen arachidis hypogaeae dregs, corn germ cake, rapeseed meal, batch, concentrate feed, Semen Maydiss and its by-product, corn, in flour extremely
Few one kind.
6. detection kit as claimed in claim 1, the mycotoxin includes aflatoxin, ochratoxin, Semen Maydiss
At least one in zeranol and its derivatives class toxin, vomitoxin and fumonisin.
7. the detection kit as described in any one of claim 1-3, the wherein solid-phase immunity chromatographic assays include
Forward at least one in light immunochromatographic method, fluorescence immune chromatography method, colloidal gold immunity chromatography and latex immunochromatographic method.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610809865.1A CN106442973A (en) | 2016-09-07 | 2016-09-07 | Fungaltoxin multi-parameter quantitative detection kit in field of food security |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610809865.1A CN106442973A (en) | 2016-09-07 | 2016-09-07 | Fungaltoxin multi-parameter quantitative detection kit in field of food security |
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ID=58164437
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| CN201610809865.1A Pending CN106442973A (en) | 2016-09-07 | 2016-09-07 | Fungaltoxin multi-parameter quantitative detection kit in field of food security |
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| CN110133311A (en) * | 2019-05-31 | 2019-08-16 | 河南冠宇仪器有限公司 | Mycotoxin rapid quantitative detection instrument |
| CN111282550A (en) * | 2020-03-03 | 2020-06-16 | 武汉轻工大学 | Preparation method and application of load type magnetic cellulose microspheres |
| CN112684180A (en) * | 2021-01-26 | 2021-04-20 | 无锡精检生物技术有限公司 | Fluorescence immunoassay quantitative POCT detection method for mycotoxin in agricultural products |
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