CN106442844A - Identification method for parched white peony root in Shiquan Dabu wine - Google Patents
Identification method for parched white peony root in Shiquan Dabu wine Download PDFInfo
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- CN106442844A CN106442844A CN201610752420.4A CN201610752420A CN106442844A CN 106442844 A CN106442844 A CN 106442844A CN 201610752420 A CN201610752420 A CN 201610752420A CN 106442844 A CN106442844 A CN 106442844A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
Abstract
The invention discloses an identification method for parched white peony root in Shiquan Dabu wine, and particularly relates to a thin-layer chromatography method with a paeoniflorin reference substance as a control and with a mixture with chloroform:ethyl acetate:methanol:formic acid=(35-45):(3-8):(8-15):(0.1-0.3) as a developer. The identification method for the parched white peony root in the Shiquan Dabu wine can efficiently and accurately identify the parched white peony root component in the Shiquan Dabu wine. The method is simple and easy to operate, strong in controllability and specificity, high in degree of separation, high in sensitivity and good in reproducibility, a negative control has no interference, and different manufacturers of thin-layer plates and temperature and humidity conditions do not affect the method. The identification method can accurately, comprehensively, scientifically and effectively identify the parched white peony root component in the bone pain medicated wine, improves quality detection standards, and further ensures the stability, safety and effectivity of the product quality,.
Description
Technical field
The present invention relates in SHIQUAN DABU JIU Radix Paeoniae Alba (parched) discrimination method, particularly belong to Chinese medicine detection technique field.
Background technology
SHIQUAN DABU JIU is by Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae (stir-fry), Poria, Radix Glycyrrhizae (processed with honey), Radix Angelicae Sinensis, Rhizoma Chuanxiong, the Radix Paeoniae Alba (stir-fry), Radix Rehmanniae Preparata
Huang, the Radix Astragali (processed with honey) and Cortex Cinnamomi are made.Adjuvant is Chinese liquor, sucrose.The function of this product is temperature compensation QI and blood.In side, Radix Rehmanniae Preparata is enriched blood taste
The moon, marrow is given birth in replenishing essence；During Radix Codonopsis spleen reinforcing is good for, QI invigorating and blood producing, Yin grows while yang is generating, altogether for monarch drug.Rhizoma Atractylodis Macrocephalae invigorating the spleen and benefiting QI；Poria spleen invigorating profit
Wet；Radix Astragali invigorating the spleen and benefiting QI yang invigorating, closes and opens source of generating QI and blood with principal drug assistance, during Radix Astragali QI invigorating is good for；Radix Angelicae Sinensis, the Radix Paeoniae Alba take a tonic or nourishing food to build up one's health the moon blood,
Sun is joined with the moon；Cortex Cinnamomi mends fire supporing yang, inspires QI and blood growth, altogether for ministerial drug.Rhizoma Chuanxiong promoting flow of QI and blood, makes benefit and not stagnant, is adjuvant drug.Sweet
Careless QI invigorating, coordinating the actions of various ingredients in a prescription, for making medicine.Ten medicine phases are closed, and play the work(of temperature compensation QI and blood altogether.In existing SHIQUAN DABU JIU quality standard,
It is not directed to the discriminating of Radix Paeoniae Alba (parched), therefore, research is a kind of easy to operate, can differentiate efficiently, rapidly to fry in vain in SHIQUAN DABU JIU
The method of Chinese herbaceous peony, is conducive to lifting the quality inspection standard of SHIQUAN DABU JIU, it appears particularly necessary.
Content of the invention
For solve the deficiencies in the prior art, it is an object of the invention to provide in a kind of SHIQUAN DABU JIU Radix Paeoniae Alba (parched) discriminating
Method, this discrimination method accuracy is good, and separating degree is high.
The SHIQUAN DABU JIU of the present invention, is by Radix Codonopsis 80g, Poria 80g, Rhizoma Atractylodis Macrocephalae (parched with bran) 80g, Radix Glycyrrhizae Preparata 40g, Radix Rehmanniae Preparata
120g, the Radix Paeoniae Alba (stir-fry) 80g, Radix Angelicae Sinensis 120g, Rhizoma Chuanxiong 40g, Radix Astragali Preparata 80g and Cortex Cinnamomi 20g make.Above ten tastes, are ground into thick
Powder, according under fluid extract and extractum item percolation (《Chinese Pharmacopoeia》Version general rule 0189 in 2015), use Chinese liquor 17200mL
Make solvent, dipping, after 48 hours, with the speed of 1～3mL per minute slowly percolation, is collected liquid of filtering, added sucrose 1720g, stir evenly,
Standing, filtration, obtain final product.
In order to realize above-mentioned target, the present invention adopts the following technical scheme that：
The discrimination method of Radix Paeoniae Alba (parched) in SHIQUAN DABU JIU, is with peoniflorin reference substance for comparison, with chloroform-acetic acid second
Ester-methyl alcohol-formic acid=35～45 3～8 8～15 0.1～0.3 is the thin layer chromatography of developing solvent.
In aforementioned SHIQUAN DABU JIU, the discrimination method of the Radix Paeoniae Alba, comprises the following steps：
(1) preparation of need testing solution：Take this product to add n-butyl alcohol shaking to extract, obtain n-butyl alcohol liquid, be evaporated, residue adds methanol
It is made to dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made and contained the comparison of 1mg peoniflorin in every 1mL
The solution of product, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance molten
Liquid, need testing solution, put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=35～
45 3～8 8～15 0.1～0.3 is developing solvent, launches, takes out, dry, spray, with 5% vanillin-sulfuric acid solution, is heated to speckle
Point colour developing is clear, in test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
Specifically, in aforementioned SHIQUAN DABU JIU Radix Paeoniae Alba (parched) discrimination method, comprise the following steps：
(1) preparation of need testing solution：Take this product 20mL～35mL, plus n-butyl alcohol 25mL～45mL shaking is extracted, and just obtains
Butanol liquid, is evaporated, and residue adds 1mL～3mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made and contained the comparison of 1mg peoniflorin in every 1mL
The solution of product, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution
9～12 μ L, need testing solution 9～12 μ L, put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-
Formic acid=35～45 3～8 8～15 0.1～0.3 is developing solvent, launches, and takes out, dries, and spray is molten with 5% vanillin-sulfuric acid
Liquid, it is clear to be heated to spot development, in test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical royal purple mottle
Point.
Preferably, in aforementioned SHIQUAN DABU JIU Radix Paeoniae Alba (parched) discrimination method, comprise the following steps：
(1) preparation of need testing solution：Take this product 20mL, plus n-butyl alcohol 30mL shaking extracted, and obtains n-butyl alcohol liquid, is evaporated,
Residue adds 1mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made and contained the comparison of 1mg peoniflorin in every 1mL
The solution of product, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution
10 μ L, need testing solution 10 μ L, put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=
40 5 10 0.2 is developing solvent, launches, takes out, dry, and spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clearly,
In test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
In order to ensure discrimination method science of the present invention, reasonable, feasible, the discrimination method of the present invention is studied and has been examined
Examine.
In side, Radix Angelicae Sinensis, the Radix Paeoniae Alba take a tonic or nourishing food to build up one's health the moon blood, join sun with the moon；Cortex Cinnamomi mends fire supporing yang, inspires QI and blood growth, altogether for ministerial drug.Side
Middle Radix Paeoniae Alba (parched) has the effect of blood-supplementing blood-nourishing, suppressing liver-YANG, easing the affected liver to relieve pain, and the peoniflorin in Radix Paeoniae Alba (parched) has liver protection, antiinflammatory, improvement
The multiple actions such as myocardial nutritional flow, expansion of blood vessels, increase coronary flow, antagonism acute myocardial ischemia, sedation and analgesia, be
Main active in preparation.Therefore, peoniflorin reference substance is chosen in this experiment is comparison, by Chinese herbaceous peony in n-butanol extraction this product
Medicine glycosides composition, carries out thin layer discriminating to Radix Paeoniae Alba (parched), and investigates different point sample amounts, different manufacturers lamellae, different temperatures, DIFFERENT WET
The impact to Radix Paeoniae Alba (parched) medical material thin layer chromatography in this product for the degree.Result of the test shows：With peoniflorin reference substance for comparison, with three chloromethanes
Alkane-acetate-methanol-formic acid=35～45 3～8 8～15 0.1～0.3 is that the thin layer chromatography feature of developing solvent is bright
Aobvious, specificity is strong, can be used as the discrimination method of Radix Paeoniae Alba (parched) in this product.
1st, instrument and reagent
MS304s/01 electronic balance (METTLER TOLEDO (prunus mume (sieb.) sieb.et zucc. Teller-support benefit)), silica gel g thin-layer plate (Qingdao Haiyang
Chemical Co., Ltd.), P-1 type thin layer chromatograph developing cylinder (company limited of Xinyi Instrument Factory, Shanghai), 5ul thin-layer sample application capillary tube
(Tianjin Si Lida Science and Technology Ltd.).
SHIQUAN DABU JIU (130901,130902,140201, Jiangsu Xin Bang pharmaceutical Co. Ltd), peoniflorin reference substance (in
State's medicine biological products assay institute), n-butyl alcohol (Xilong Chemical Co., Ltd, analysis pure), (western Gansu Province chemical industry share has methanol
Limit company, analysis is pure), chloroform (Xilong Chemical Co., Ltd, analysis pure), (western Gansu Province chemical industry share has ethyl acetate
Limit company, analysis is pure), formic acid (Chengdu Kingsoft chemical reagent company limited, analysis pure), (Tianjin section is close europeanized to learn examination to vanillin
Agent company limited, analysis is pure), sulphuric acid (Tianjin Ke Miou chemical reagent company limited, analysis pure).
2nd, the investigation of discrimination method
2.1st, the preparation method of need testing solution
In prior art, when Radix Paeoniae Alba (parched) differentiates, need testing solution is to be processed by first adding ethanol shaking, filters, filtering residue adds second
Alcohol dissolving obtains.Compared with prior art, adopt n-butyl alcohol as Extraction solvent in the present invention, extraction time is short, extracts effect
Really good.Prepare need testing solution respectively using two methods, under equal conditions differentiated, using a component of prior art
Poor from spending, and adopt one group of separating degree height of the inventive method, favorable reproducibility.
2.2nd, the investigation of optimal point sample amount
Need testing solution and reference substance solution 6,7,8,9,10,12,14 μ L in difference aspiration step (1) and step (2), point
Other point, on different silica gel g thin-layer plates, is investigated, and with chloroform-acetate-methanol-formic acid=40 5 10 0.2 is
Developing solvent, launches, and takes out, dries, and spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clear.Wherein, test sample 10 μ
L point sample amount best results, when point sample amount is more than 12 μ L, conditions of streaking in need testing solution position speckle, and point sample amount is less than 9
During μ L, need testing solution position speckle is inconspicuous；During reference substance solution point sample amount 9～12 μ L, effect is good, especially in 10 μ L
Best results, therefore select need testing solution point sample amount to be 9～12 μ L, optimal point sample amount 10 μ L；Reference substance solution point sample amount 9～12
μ L, optimal point sample amount 10 μ L.
3rd, specificity is investigated
In need testing solution 10 μ L in aspiration step (1), step (2), reference substance solution 10 μ L, Radix Paeoniae Alba (parched) negative control are molten
Liquid 10 μ L, puts on same silica gel g thin-layer plate by need testing solution, reference substance solution, negative control solution, with three chloromethanes respectively
Alkane-acetate-methanol-formic acid=40 5 10 0.2 are developing solvent, launch, and take out, dry, and spray is molten with 5% vanillin-sulfuric acid
Liquid, is heated to spot development clear.As shown in Figure 1.In test sample chromatograph, with reference substance chromatograph relevant position on, show identical
Bluish violet speckle, immaculate on relevant position in negative control chromatograph, show that the specificity of the method is good.
4th, ruggedness is investigated
4.1 different manufacturers lamellaes
In need testing solution 10 μ L in aspiration step (1), step (2), reference substance solution 10 μ L, Radix Paeoniae Alba (parched) negative control are molten
Liquid 10 μ L, by need testing solution, reference substance solution, negative control solution point sample respectively, changes the lamellae of different manufacturers, with three
Chloromethanes-acetate-methanol-formic acid=40 5 10 0.2 are developing solvent, launch, and take out, dry, and spray is with 5% vanillin sulfur
Acid solution, is heated to spot development clear.In test sample chromatograph, on position corresponding with reference substance chromatograph, aobvious identical is blue
Purple dot, after immaculate on relevant position in Radix Paeoniae Alba (parched) negative control chromatograph, and replacing different manufacturers lamellae, each lamellae
Between no significant difference, show that changing different manufacturers lamellae differentiates no to affect on the thin layer of Radix Paeoniae Alba (parched).As shown in Figure 2.
4.2 humiture
In need testing solution 10 μ L in aspiration step (1), step (2), reference substance solution 10 μ L, Radix Paeoniae Alba (parched) negative control are molten
Liquid 10 μ L, by need testing solution, reference substance solution, negative control solution point sample respectively, the different temperature and humidity conditions of conversion, launches,
Take out, dry, spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clear.In test sample chromatograph, with reference substance color
Compose on corresponding position, aobvious identical bluish violet speckle, immaculate on relevant position in Radix Paeoniae Alba (parched) negative control chromatograph, and convert
After different temperature and humidity conditions, no significant difference between each lamellae, show that converting different humitures differentiates to the thin layer of Radix Paeoniae Alba (parched)
No affect.As shown in Figure 3.
The invention has benefit that：The discrimination method of Radix Paeoniae Alba (parched), Neng Gougao in the SHIQUAN DABU JIU that the present invention provides
Radix Paeoniae Alba (parched) composition in effect, exactly discriminating SHIQUAN DABU JIU.The method of the present invention, simple to operation, controllability, specificity are strong,
Separating degree is high, and sensitivity is high, favorable reproducibility, and negative control is noiseless, and different manufacturers lamellae and temperature, damp condition are equal
Do not affect.Discrimination method of the present invention can carry out accurate, comprehensive, scientifical effective discriminating to Radix Paeoniae Alba (parched) composition in ostealgia medicated wine,
Improve quality inspection standard, further ensure that the stable, safely, effectively of product quality.
Brief description
Fig. 1 be the present invention SHIQUAN DABU JIU in Radix Paeoniae Alba (parched) specificity differentiate chromatogram；
Fig. 2 is chromatogram when Radix Paeoniae Alba (parched) differentiates in SHIQUAN DABU JIU using different lamellaes；
Fig. 3 is the chromatogram under different temperature and humidity conditions when Radix Paeoniae Alba (parched) differentiates in SHIQUAN DABU JIU；
The implication of in figure reference：Fig. 1～Fig. 3:1- test sample lot number 130901,2- test sample lot number 130902,3-
Test sample lot number 140201,4- peoniflorin reference substance, 5- Radix Paeoniae Alba (parched) negative control；Fig. 2：A- Qingdao Haiyang silica gel g thin-layer plate (rule
Lattice：100 × 200mm), B- Yantai Chemical Industry Research Inst. silica gel g thin-layer plate (specification：100×200mm)；Fig. 3：A- temperature
28.7 DEG C, humidity 32%, 3.6 DEG C of B- temperature, humidity 72%.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is further introduced.
Embodiment 1
In SHIQUAN DABU JIU, the discrimination method of Radix Paeoniae Alba (parched), comprises the following steps：
(1) preparation of need testing solution：Take this product 25mL, plus n-butyl alcohol 25mL shaking extracted, and obtains n-butyl alcohol liquid, is evaporated,
Residue adds 2mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made and contained the comparison of 1mg peoniflorin in every 1mL
The solution of product, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution
9 μ L, need testing solution 9 μ L, put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=
35 38 0.1 is developing solvent, launches, takes out, dry, and spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clearly, supplies
In test product chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
Embodiment 2
In SHIQUAN DABU JIU, the discrimination method of Radix Paeoniae Alba (parched), comprises the following steps：
(1) preparation of need testing solution：Take this product 35mL, plus n-butyl alcohol 45mL shaking extracted, and obtains n-butyl alcohol liquid, is evaporated,
Residue adds 3mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made and contained the comparison of 1mg peoniflorin in every 1mL
The solution of product, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution
12 μ L, need testing solution 12 μ L, put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=
45 8 15 0.3 is developing solvent, launches, takes out, dry, and spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clearly,
In test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
Embodiment 3
In SHIQUAN DABU JIU, the discrimination method of Radix Paeoniae Alba (parched), comprises the following steps：
(1) preparation of need testing solution：Take this product 20mL, plus n-butyl alcohol 30mL shaking extracted, and obtains n-butyl alcohol liquid, is evaporated,
Residue adds 1mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made and contained the comparison of 1mg peoniflorin in every 1mL
The solution of product, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution
10 μ L, need testing solution 10 μ L, put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=
40 5 10 0.2 is developing solvent, launches, takes out, dry, and spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clearly,
In test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
Embodiment 4
In SHIQUAN DABU JIU, the discrimination method of Radix Paeoniae Alba (parched), comprises the following steps：
(1) preparation of need testing solution：Take this product 30mL, plus n-butyl alcohol 40mL shaking extracted, and obtains n-butyl alcohol liquid, is evaporated,
Residue adds 2mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made and contained the comparison of 1mg peoniflorin in every 1mL
The solution of product, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution
11 μ L, need testing solution 10 μ L, put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=
38 6 12 0.2 is developing solvent, launches, takes out, dry, and spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clearly,
In test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
Claims (4)
1. in SHIQUAN DABU JIU Radix Paeoniae Alba (parched) discrimination method it is characterised in that：Described discrimination method is to be with peoniflorin reference substance
Comparison, with chloroform-thin layer as developing solvent for acetate-methanol-formic acid=35～45 3～8 8～15 0.1～0.3
Chromatography.
2. in SHIQUAN DABU JIU according to claim 1 Radix Paeoniae Alba (parched) discrimination method it is characterised in that：Specific discriminating side
Method comprises the following steps：
(1) preparation of need testing solution：Take this product to add n-butyl alcohol shaking to extract, obtain n-butyl alcohol liquid, be evaporated, residue adds methanol makes it
Dissolving, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made in every 1mL containing 1mg peoniflorin reference substance
Solution, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution, confession
Test sample solution, puts respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=35～45 3～
8 8～15 0.1～0.3 is developing solvent, launches, takes out, dry, spray, with 5% vanillin-sulfuric acid solution, is heated to spot development
Clearly, in test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
3. in SHIQUAN DABU JIU according to claim 2 Radix Paeoniae Alba (parched) discrimination method it is characterised in that：Specific discriminating side
Method comprises the following steps：
(1) preparation of need testing solution：Take this product 20mL～35mL, plus n-butyl alcohol 25mL～45mL shaking is extracted, and obtains n-butyl alcohol
Liquid, is evaporated, and residue adds 1mL～3mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made in every 1mL containing 1mg peoniflorin reference substance
Solution, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution 9～
12 μ L, need testing solution 9～12 μ L, put on same silica gel g thin-layer plate, with chloroform-acetate-methanol-first respectively
Acid=35～45 3～8 8～15 0.1～0.3 is developing solvent, launches, and takes out, dries, and sprays with 5% vanillin-sulfuric acid solution,
Be heated to spot development clear, in test sample chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
4. in the SHIQUAN DABU JIU according to Claims 2 or 3 Radix Paeoniae Alba (parched) discrimination method it is characterised in that：Specific mirror
Other method comprises the following steps：
(1) preparation of need testing solution：Take this product 20mL, plus n-butyl alcohol 30mL shaking is extracted, and obtains n-butyl alcohol liquid, is evaporated, residue
Plus 1mL methanol makes it dissolve, as need testing solution；
(2) preparation of reference substance solution：Take peoniflorin reference substance, plus methanol is made in every 1mL containing 1mg peoniflorin reference substance
Solution, as reference substance solution；
(3) differentiate：According to《Chinese Pharmacopoeia》Four general rule 0502 thin layer chromatography tests of version in 2015, draw reference substance solution 10 μ
L, need testing solution 10 μ L, put on same silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid=40 respectively
5 10 0.2 is developing solvent, launches, takes out, dry, and spray, with 5% vanillin-sulfuric acid solution, is heated to spot development clear, for examination
In product chromatograph, with reference substance chromatograph relevant position on, aobvious identical bluish violet speckle.
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| CN112903844A (en) * | 2021-01-19 | 2021-06-04 | 皖西学院 | Detection method for content of pteridophytin in fiddlehead |
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Application publication date: 20170222 |