CN106442684A - Dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device - Google Patents
Dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device Download PDFInfo
- Publication number
- CN106442684A CN106442684A CN201610667456.2A CN201610667456A CN106442684A CN 106442684 A CN106442684 A CN 106442684A CN 201610667456 A CN201610667456 A CN 201610667456A CN 106442684 A CN106442684 A CN 106442684A
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- China
- Prior art keywords
- piston
- cylinder
- piston rod
- dropping device
- sealing
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
Abstract
The invention discloses a dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device. The dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device comprises a transparent cylinder. A liquid outlet is formed in the lower end of the cylinder, a piston capable of axially moving along the cylinder is arranged in the cylinder, the piston is fixedly connected with one end of a piston rod, and the other end of the piston rod extends to the outer side of the cylinder; a communicated air hole is formed in the axial center position of the piston and the piston rod; a hand wheel is connected to the end of the piston rod, and a part for closing and opening the air hole is arranged on the end head of the piston rod; scales are arranged at the portion, located at the piston axial moving area, of the outer side of the cylinder; a piston axial-moving-position positioning piece is arranged in the cylinder. When the dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device is used, an upper end opening of the air hole is closed, oil liquid is absorbed into the cylinder by the liquid outlet through moving-up movement of the piston, then an opening closing part of the air hole is opened, the oil liquid can drop from the liquid outlet, the dropping speed of the time interval of the oil liquid can be adjusted through the opened size of the air hole, the oil liquid automatically and evenly drops, artificial control is not required, and time and labor are saved. The dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device is simple in structure and easy to control.
Description
Technical field
The present invention relates to a kind of drop oiler, particularly a kind of two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device, main use
In two-dimensional electrophoresis mode, protein is carried out with quantitative oil dripping when analyzing, to form adhesive tape aquation sealing.
Background technology
Two dimensional polyacrylamide gel electrophoresis technology combines isoelectric focusing technique(Carried out point according to isoelectric points of proteins
From)And SDS- technique of polyacrylamide gel electrophoresis(Size according to protein carries out separating), this two technology combine shape
The two dimensional electrophoresises becoming are to separate a kind of maximally effective electrophoresis means of analysing protein.During above-mentioned two-dimensional electrophoresis, wherein
Adhesive tape aquation sealing need to be sealed by the way of oil dripping.How to carry out oil dripping using manually mode, not only at present
Waste time and energy, and uneven during lower of fluid, all uneven including the position and the time interval falling of respectively dripping dripped, to adhesive tape
Aquation effect and follow-up Protein Separation can produce certain impact.
Content of the invention
It is an object of the invention to provide a kind of structure is simple, easily manipulation, oil dripping are uniform, time saving and energy saving and in terms of oil dripping
The two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device that two-dimensional electrophoresis process is not had a negative impact.
The two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device of the present invention, including the cylinder of transparent material, under cylinder
End is provided with liquid outlet, is furnished with and can be fixedly connected with one end of piston rod along the piston of cylinder axial movement, piston in described cylinder,
The other end of piston rod extends the outside of cylinder;The pore being communicated with the axial location of piston and piston rod;In piston rod
End be connected to handwheel, the part that the termination of piston rod is provided with closing and opens pore;The outside of described cylinder is located at piston shaft
It is provided with scale to moving area;It is provided with piston axial direction shift position keeper in cylinder.
The two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device of the present invention, wherein said piston axial direction shift position positions
Part includes connecting on the piston rod and be located at the rotating disk above piston, is screw thread between the outer periphery of rotating disk and cylinder medial surface
Connect.
The two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device of the present invention, wherein said piston axial direction shift position positions
Part includes connecting on the piston rod and be located at the protruding block above piston, and cylinder medial wall is longitudinally provided with the gear at multiple intervals
Plate, the described thickness of protruding block is matched with the spacing of adjacent baffle plate.
The two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device of the present invention, wherein said closing and the part opening pore
Including the flexible pipe being sleeved on piston rod end, regulation folder is provided with flexible pipe.
The two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device of the present invention, wherein said closing and the part opening pore
Including rotating plectane, rotate plectane and be supported on above handwheel by shaft parallel, rotating plectane with handwheel is in eccentric structure, rotates
Plectane just covers the upper end surface in piston rod, is provided with circular hole on rotating plectane, the vertical dimension phase of circular hole, pore and rotating shaft
Deng.
The two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device of the present invention, wherein said closing and the part opening pore
Including offering sinking arcuate groove on handwheel, in arcuate groove, it is provided with slide block, open-work is provided with slide block, slide block is in arcuate groove
During interior slip, open-work can be overlapped with the upper port of pore or be staggered.
The upper port of pore during use, is first closed, profit by the two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device of the present invention
With the action moving on piston, fluid is sucked into cylinder by liquid outlet, piston movement be can determine that according to the scale on cylinder
Final position is put, and after piston positioning, by support, this drop oiler is supported on certain altitude, and the lower section of liquid outlet is to need to instill fluid
Container, then open the sealing part of pore upper end, fluid dropwise can fall from liquid outlet, pore upper port open size
Speed and time interval that adjustable fluid is lower, after adjustment is suitable, fluid can drip automatically and uniformly, need not artificially control
System, time saving and energy saving, this drop oiler structure is simple, easily manipulates.
Brief description
Fig. 1 is the structural representation of embodiment 1 in the specific embodiment of the invention;
Fig. 2 is the structural representation of embodiment 2 in the specific embodiment of the invention;
Fig. 3 is the A-A section structure schematic diagram shown in Fig. 2;
Fig. 4 is the K shown in Fig. 2 to schematic diagram;
Fig. 5 is the structural representation of embodiment 3 in the specific embodiment of the invention;
Fig. 6 is the D shown in Fig. 5 to schematic diagram.
Specific embodiment
Embodiment 1:As shown in Figure 1:1 is the cylindrical tube of transparent material, in the lower end of cylinder 1 by pyramidal structure even
It is connected to liquid outlet 3.Being furnished with cylinder 1 can be along the piston 4 of cylinder 1 axial movement.Piston 4 is fixedly connected with one end of piston rod 6.
The other end of piston rod 6 extends the outside of cylinder 1.Axial centre position or off-centered position in piston 4 and piston rod 6
The pore 5 being communicated with.
It is connected to handwheel 9 in the end of piston rod 6, by the rotatable piston rod 6 of handwheel 9 and piston 4.The termination of piston rod 6
The part being provided with closing and opening pore 5.The part of closing and unlatching pore 5 includes being sleeved on the flexible pipe 10 of piston rod 6 termination,
Regulation folder 11 is provided with flexible pipe 10, the roller type adjusting the adjustment medicinal liquid dropping velocity on the optional hose of infusion apparatus of folder 11 is adjusted
Whole folder or the adjustable holder of other forms, as long as the adjustable clamping degree to flexible pipe 10 is it is ensured that flexible pipe 10 is herein
Close or open.
The outside of cylinder 1 is located at piston 4 axial movement region and is provided with scale 2.It is provided with piston 4 to move axially in cylinder 1
Location positioning part.Piston 4 moves axially the rotating disk 8 that location positioning part includes being connected on piston rod 6 and being located at piston 4 top,
It is to threaded between the outer periphery of rotating disk 8 and cylinder 1 medial surface.
During use, first the upper port of pore 5 is passed through to adjust folder 11 closing, rotate handwheel 9, rotating disk 8 and piston 4 turn therewith
Dynamic, using the action moving on piston 4, fluid is sucked into cylinder 1 by liquid outlet 3, work be can determine that according to the scale 2 on cylinder 1
This drop oiler, after piston 4 positioning, is supported on certain altitude, the lower section of liquid outlet 3 by support by the final position of plug 4 movements
It is the container needing to instill fluid, then opens regulation folder 11, fluid dropwise can fall from liquid outlet, and pore 5 upper port is opened
Size can adjust the speed of lower of fluid and time interval, when adjustment properly after, fluid can drip automatically and uniformly, without people
For controlling, time saving and energy saving, this drop oiler structure is simple, easily manipulates.
Embodiment 2
As shown in Figure 2,3, 4:Include being connected on piston rod 6 from piston unlike embodiment 4 axial movement location positioning part
And the protruding block 15 positioned at piston 4 top, cylinder 1 medial wall is longitudinally provided with the baffle plate 14 at multiple intervals, the thickness of protruding block 15
Degree is matched with the spacing of adjacent baffle plate 14.
The part of closing and unlatching pore 5 includes rotating plectane 13, rotates plectane 13 and passes through rotating shaft 12 parallel support in handss
Above wheel 9, rotating plectane 13 with handwheel 9 is in eccentric structure, rotates plectane 13 and just covers in the upper end surface of piston rod 6, is turning
Dynamic plectane 13 is provided with circular hole 16, and circular hole 16, pore 5 are equal with the vertical dimension of rotating shaft 12.
During use, rotate rotor plate 13, the upper port of pore 5 is closed by rotor plate 13, and that is, circular hole 16 and pore 5 are staggered
Open position.Rotate handwheel 9, protruding block 15 leaves baffle plate 14, you can pull piston 4, using the action moving on piston 4 by going out liquid
Mouth 3 sucks fluid into cylinder 1, can determine that the final position of piston 4 movement according to the scale 2 on cylinder 1, then passes through handwheel
9 rotation piston rods 6, make protruding block enter in the gap between one pair of which baffle plate 14, so that piston 4 is positioned, and incite somebody to action this by support
Drop oiler is supported on certain altitude, and the lower section of liquid outlet 3 is the container needing to instill fluid, then rotates rotor plate 13, makes circle
Hole 16 is overlapped with pore 5, and fluid dropwise can fall from liquid outlet 3, lower of how many adjustable fluid that pore 5 is overlapped with circular hole 16
Speed and time interval, after adjustment is suitable, fluid can drip automatically and uniformly, without manual control, time saving and energy saving, this
Oily device structure is simple, easily manipulates.
Embodiment 3
As shown in Figure 5,6, under the part of closing and unlatching pore 5 includes offering on handwheel 9 as different from Example 2
Heavy arcuate groove 18, is provided with slide block 17 in arcuate groove 18, be provided with open-work 7 on slide block 17, when slide block 17 slides in arcuate groove 18
Open-work 7 can be overlapped with the upper port of pore 5 or be staggered, many or few, the speed that adjustable fluid is lower of the part that overlaps, its
He is same as Example 2, is not described in detail.
Claims (6)
1. a kind of two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device, it is held to levy and is:Cylinder including transparent material(1),
Cylinder(1)Lower end be provided with liquid outlet(3), described cylinder(1)Inside being furnished with can be along cylinder(1)The piston of axial movement(4), piston
(4)With piston rod(6)One end be fixedly connected, piston rod(6)The other end extend cylinder(1) outside;In piston(4)And work
Stopper rod(6)The pore that is communicated with of axial location(5);In piston rod(6)End be connected to handwheel(9), piston rod(6)End
Head is provided with closing and opens pore(5)Part;Described cylinder(1)Outside be located at piston(4)Axial movement region is provided with quarter
Degree(2);In cylinder(1)Inside it is provided with piston(4)Axial movement location positioning part.
2. two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device according to claim 1, it is held to levy and is:Described piston
(4)Axial movement location positioning part includes being connected to piston rod(6)Go up and be located at piston(4)The rotating disk of top(8), rotating disk(8)
Outer periphery and cylinder(1)It is threaded between medial surface.
3. two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device according to claim 1, it is held to levy and is:Described piston
(4)Axial movement location positioning part includes being connected to piston rod(6)Go up and be located at piston(4)The protruding block of top(15), cylinder
(1)The baffle plate at multiple intervals is longitudinally provided with medial wall(14), described protruding block(15)Thickness and adjacent baffle plate(14)
Spacing match.
4. the two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device according to claim 1 or 2 or 3 it is characterised in that:Described
Closing and open pore(5)Part include being sleeved on piston rod(6)The flexible pipe of termination(10), in flexible pipe(10)It is provided with tune
Section folder(11).
5. the two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device according to claim 1 or 2 or 3 it is characterised in that:Described
Closing and open pore(5)Part include rotate plectane(13), rotate plectane(13)By rotating shaft(12)Parallel support exists
Handwheel(9)Top, rotates plectane(13)With handwheel(9)In eccentric structure, rotate plectane(13)Just cover in piston rod(6)Upper
End, is rotating plectane(13)It is provided with circular hole(16), circular hole(16), pore(5)With rotating shaft(12)Vertical dimension equal.
6. the two-dimensional electrophoresis adhesive tape aquation sealing quantitative oil-dropping device according to claim 1 or 2 or 3 it is characterised in that:Described
Closing and open pore(5)Part include in handwheel(9)Offer sinking arcuate groove above(18), arcuate groove(18)Inside set
There is slide block(17), in slide block(17)It is provided with open-work(7), slide block(17)In arcuate groove(18)Open-work during interior slip(7)Can be with gas
Hole(5)Upper port overlap or be staggered.
Priority Applications (1)
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CN201610667456.2A CN106442684B (en) | 2016-08-15 | 2016-08-15 | Dielectrophoresis adhesive tape aquation sealing quantitative oil-dropping device |
Applications Claiming Priority (1)
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CN201610667456.2A CN106442684B (en) | 2016-08-15 | 2016-08-15 | Dielectrophoresis adhesive tape aquation sealing quantitative oil-dropping device |
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CN106442684A true CN106442684A (en) | 2017-02-22 |
CN106442684B CN106442684B (en) | 2018-10-19 |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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EP1469068A1 (en) * | 2003-03-28 | 2004-10-20 | Fuji Photo Film Co. Ltd. | Apparatus for separating and purifying nucleid acid and method for separating and purifying nucleid acid |
CN102595963A (en) * | 2009-10-19 | 2012-07-18 | 株式会社衍宇 | The spuit controlling the quantity of its content and the cosmetic container therewith |
CN205199535U (en) * | 2015-11-11 | 2016-05-04 | 宁波卫生职业技术学院 | Take glass piston's scale to inhale buret |
CN105618169A (en) * | 2015-12-21 | 2016-06-01 | 安徽农业大学 | Equivalent continuous dropping liquid-transferring gun |
CN205361385U (en) * | 2016-01-21 | 2016-07-06 | 中华人民共和国伊犁出入境检验检疫局 | With supporting pipettor that uses of pipette |
CN205982167U (en) * | 2016-08-15 | 2017-02-22 | 大连医科大学 | Dimensional electrophoresis adhesive tape aquation seal ration drop feed oiler |
-
2016
- 2016-08-15 CN CN201610667456.2A patent/CN106442684B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1469068A1 (en) * | 2003-03-28 | 2004-10-20 | Fuji Photo Film Co. Ltd. | Apparatus for separating and purifying nucleid acid and method for separating and purifying nucleid acid |
CN102595963A (en) * | 2009-10-19 | 2012-07-18 | 株式会社衍宇 | The spuit controlling the quantity of its content and the cosmetic container therewith |
CN205199535U (en) * | 2015-11-11 | 2016-05-04 | 宁波卫生职业技术学院 | Take glass piston's scale to inhale buret |
CN105618169A (en) * | 2015-12-21 | 2016-06-01 | 安徽农业大学 | Equivalent continuous dropping liquid-transferring gun |
CN205361385U (en) * | 2016-01-21 | 2016-07-06 | 中华人民共和国伊犁出入境检验检疫局 | With supporting pipettor that uses of pipette |
CN205982167U (en) * | 2016-08-15 | 2017-02-22 | 大连医科大学 | Dimensional electrophoresis adhesive tape aquation seal ration drop feed oiler |
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