CN106442452A - Fluorescence detection device based on liquid optical device in micro-fluidic chip and detection method - Google Patents
Fluorescence detection device based on liquid optical device in micro-fluidic chip and detection method Download PDFInfo
- Publication number
- CN106442452A CN106442452A CN201610949502.8A CN201610949502A CN106442452A CN 106442452 A CN106442452 A CN 106442452A CN 201610949502 A CN201610949502 A CN 201610949502A CN 106442452 A CN106442452 A CN 106442452A
- Authority
- CN
- China
- Prior art keywords
- micro
- fluidic chip
- liquid
- liquid optical
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Abstract
The invention discloses a fluorescence detection device based on a liquid optical device in a micro-fluidic chip and a detection method. The device comprises a light source, a bearing platform, the micro-fluidic chip, an optical detection component, a signal processing component and a display component. Adjustable devices, such as liquid lenses and a liquid optical filter, are adopted, so that the device can be adjusted according to the experiment requirement in a detection process, the to-be-filtered fluorescent light in the wavelength which is not required by the experiment can be adjusted by adjusting the liquid in the liquid optical filter, the focal length of the liquid lenses can be adjusted by adjusting the voltage, the interior hardware need not be frequently changed in the detection process and the detection becomes convenient. According to the invention, the liquid optical filter and the three liquid lenses are arranged on the two sides of a micro-channel and are extremely close to a to-be-detected matter and the fluorescent light is collected once being stimulated, so that most fluorescent light can be collected and the fluorescence intensity can be enhanced. When the granular matter is detected, the fluorescence signal which cannot be detected due to the fluorescent scattering can be avoided.
Description
Technical field
The present invention relates to a kind of detection technique of white light sample, particularly one kind is based on liquid optical device in micro-fluidic chip
Fluorescence detection device and detection method.
Background technology
At present, flow cytometer (flow cytometry, FCM) is that the cell of high-speed straight-line flowing or biological particle are entered
Row quantitative determination and the instrument of analysis, are also applied for detection and the analysis of fluorescent samples, the main flowing skill including sample
Art, the counting of cell and sorting technology, computer is to the collection of data and analytical technology etc..Flow cytometer is with flow cytometry
For theoretical basiss, it is the subjects such as hydrodynamics, laser technology, electronic engineering, molecular immunology, cell fluorescence and computer
The crystallization of knowledge integration application.It is characterized in：Measuring speed is fast, tested colony big, can carry out measuring multiple parameters, in biological, doctor
Field is widely used.But this equipment instrument huge, expensive it has not been convenient to detection, be also unfavorable for extensively applying.
For disadvantage mentioned above, there has been proposed a kind of portable flow cytometer based on micro-fluidic chip, micro-fluidic core
The units such as pretreatment, reaction, separation and detection are integrated in one single chip piece (microfluidic chip) technology, have
The features such as integrated level height, small volume, represent the direction of miniaturization Instrument Development.Using detection technique be optical detection, tool
The light source of body is LED light source, the maturation with technology and the improvement of performance, has been able to provide high power, short wavelength, monochromaticity
And the LED light source having good stability.Using LED as excitation source can reduces cost, reduce volume be more conducive to portable integrated.
Light detecting device and display device also select miniaturization equipment.Had integrated based on the portable flow cytometer of micro-fluidic chip
Degree height, low cost, the advantage being adapted to Site Detection.But during the detection of this instrument, light can only be passed through by above thing to be detected
Thing to be detected is irradiated to lower section, leads to detection inconvenient, and if there are multiple things to be detected, then need to change the focal length of lens and
Change optical filter, increased the complexity of detection, and fluorescence is outside scattering in detection process, and fluorescence intensity is
It is inversely proportional to distance, increases strength reduction with distance, so the fluorescence signal intensity generally obtaining in the detection is inadequate.
Content of the invention
For solve prior art exist the problems referred to above, the present invention to design a kind of more convenient detection based on micro-fluidic core
The fluorescence detection device of liquid optical device and detection method in piece
To achieve these goals, technical scheme is as follows：
A kind of fluorescence detection device based on liquid optical device in micro-fluidic chip, including light source, carrying platform, micro-fluidic
Chip, photodetection assembly, signal processing component and display module；Described micro-fluidic chip is fixed in carrying platform, described light
Source is located on carrying platform, and described photodetection assembly is fixed on outside carrying platform and micro-fluidic chip is in same level
On, described photodetection assembly, signal processing component and display module are connected by signal cable sequential series；
Described light source is LED light source, and described micro-fluidic chip is made up of substrate and cover plate, together with substrate is sealed to cover plate
Form microchannel, described substrate is polydimethylsiloxane or polymethyl methacrylate, and described cover plate is sheet glass；
The microchannel both sides of described micro-fluidic chip respectively arrange one group of liquid optical processing assembly, described liquid optical processing assembly
Including a liquid optical filter and three liquid lens, described liquid optical filter and three liquid lens from the side of microchannel by
Arrange along the vertical direction order of microchannel from inside to outside, two groups of liquid optical processing assemblies are symmetrically distributed in the both sides of microchannel；Institute
Stating liquid lens is the controlled lens of voltage, is made up of water phase and an oil phase, has and changes focal length of lens function by voltage；Described liquid
Optical filter is controlled optical filter, has to drive by voltage and realizes multi-wavelength's regulatory function.
Based on the detection method of the fluorescence detection device of liquid optical device in micro-fluidic chip, comprise the following steps：
A, material to be detected is put in the microchannel of micro-fluidic chip, symmetrically put in the microchannel both sides of micro-fluidic chip
Put liquid optical processing assembly；
B, the thing to be detected in the microchannel that the parallel rayies that LED light source collimator shoots out are irradiated to micro-fluidic chip
In matter, and the fluorescence of multi-wavelength is inspired on material to be detected；
C, the light mixing natural light and fluorescence filter natural light and the wavelength model of experiment needs through liquid optical filter
After enclosing outer fluorescence, the fluorescence in wave-length coverage that experiment is needed is transferred to three liquid lens successively, and is finally transferred to
Photodetection assembly；
The fluorescence conversion transmitting is the corresponding signal of telecommunication by D, photodetection assembly, and is sent to signal processing component；
E, signal processing component are filtered processing and amplifying to the signal of telecommunication, and suppression noise amplifies signal, improves signal to noise ratio；
Signal after signal processing component is processed is shown by F, display module.
The operation principle of part of devices of the present invention is as follows：
Liquid lens are the self-control controlled lens of voltage, are made up of water phase and an oil phase, change voltage and can change liquid lens Jiao
Away from realizing varifocal function；Liquid optical filter is to make controlled optical filter by oneself, is driven by voltage, realizes multi-wavelength controlled.
Compared with prior art, the invention has the advantages that：
1st, the present invention passes through using tunable arrangements such as liquid lens, liquid optical filters, can be according to reality in detection process
Test demand adjustment, the liquid in adjustment liquid optical filter is electric to adjust the fluorescence needing the experiment filtering not need wavelength, adjustment
Pressure, to adjust the focal length of liquid lens so that not needing in detection process frequently to change internal hardware, is convenient to detect.
2nd, liquid optical filter and three liquid lens are positioned over microchannel both sides by the present invention, are extremely close to thing to be detected
Matter, fluorescence just has been excited out and has just been collected process so that fluorescence major part is collected, and enhances fluorescence intensity, little detecting
During particulate matter, will not cause because of fluorescent scattering to can't detect fluorescence signal.
Brief description
The present invention has 3, accompanying drawing, wherein：
Fig. 1 is the fluorescence detection device structural representation of liquid optical device in micro-fluidic chip.
Fig. 2 is the structural representation of liquid optical processing assembly.
Fig. 3 is microfluidic chip structure schematic diagram.
In figure：1st, light source, 2, carrying platform, 3, micro-fluidic chip, 4, photodetection assembly, 5, signal processing component, 6, aobvious
Show assembly, 7, liquid optical processing assembly, 8, liquid optical filter, 9, liquid lens.
Specific embodiment
The invention will be further described with example below in conjunction with the accompanying drawings.As seen from Figure 1, the present invention is flat by light source 1, carrying
Platform 2, micro-fluidic chip 3, photodetection assembly 4, signal processing component 5 and display module 6 form.By Fig. 2 finding, in micro-fluidic core
In piece 3, liquid optical filter 8 and liquid lens group 9 are arranged in micro-fluidic chip 3 in order.Irradiate when light source 1 produces light beam
On the microchannel of micro-fluidic chip 3, when there being test substance to pass through in microchannel, it is glimmering that light source 1 can excite test substance to produce
Light, natural light mixing fluorescence passes through liquid optical filter 8, after filtering the fluorescence outside the wave-length coverage that natural light and experiment need, will
The fluorescence in wave-length coverage that experiment needs is transferred to three liquid lens 9 successively, and liquid lens 9 are adjusted to properly by focusing
Focal length, fluorescence is sent to photodetection assembly 4.The body of testing equipment can be greatly reduced by these liquid optics
Long-pending, and do not need often to convert device therein it is only necessary to adjust the face that voltage just can change focal length and liquid optical filter 9
Color, greatly reduces time and the complexity of detection.
Claims (2)
1. a kind of fluorescence detection device based on liquid optical device in micro-fluidic chip it is characterised in that：Including light source (1), hold
Carrying platform (2), micro-fluidic chip (3), photodetection assembly (4), signal processing component (5) and display module (6)；Described micro-fluidic
Chip (3) is fixed in carrying platform (2), and described light source (1) is located on carrying platform (2), described photodetection assembly (4)
It is fixed on carrying platform (2) outward and micro-fluidic chip (3) is in same level, at described photodetection assembly (4), signal
Reason assembly (5) and display module (6) are connected by signal cable sequential series；
Described light source (1) is LED light source, and described micro-fluidic chip (3) is made up of substrate and cover plate, and substrate and cover plate are sealed to one
Rise and form microchannel, described substrate is polydimethylsiloxane or polymethyl methacrylate, and described cover plate is sheet glass；
The microchannel both sides of described micro-fluidic chip (3) respectively arrange one group of liquid optical processing assembly (7), described liquid optical processing group
Part (7) includes a liquid optical filter (8) and three liquid lens (9), described liquid optical filter (8) and three liquid lens
(9) arrange along the vertical direction order of microchannel from inside to outside from the side of microchannel, two groups of liquid optical processing assembly (7) are symmetrical
It is distributed in the both sides of microchannel；Described liquid lens (9) are the controlled lens of voltage, are made up of water phase and an oil phase, have by voltage
Change focal length of lens function；Described liquid optical filter (8) is controlled optical filter, has to drive by voltage and realizes multi-wavelength's tune
Section function.
2. the detection method based on the fluorescence detection device of liquid optical device in micro-fluidic chip, comprises the following steps：
A, material to be detected is put in the microchannel of micro-fluidic chip (3), symmetrical in the microchannel both sides of micro-fluidic chip (3)
Place liquid optical processing assembly (7)；
B, the thing to be detected being irradiated to from the parallel rayies that LED light source collimator shoots out in the microchannel of micro-fluidic chip (3)
In matter, and the fluorescence of multi-wavelength is inspired on material to be detected；
C, the light mixing natural light and fluorescence filter natural light and the wave-length coverage of experiment needs through liquid optical filter (8)
After outer fluorescence, the fluorescence in wave-length coverage that experiment is needed is transferred to three liquid lens (9) successively, and is finally transferred to
Photodetection assembly (4)；
The fluorescence conversion transmitting is the corresponding signal of telecommunication by D, photodetection assembly (4), and is sent to signal processing component
(5)；
E, signal processing component (5) are filtered processing and amplifying to the signal of telecommunication, and suppression noise amplifies signal, improves signal to noise ratio；
Signal after signal processing component (5) is processed is shown by F, display module (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610949502.8A CN106442452A (en) | 2016-11-02 | 2016-11-02 | Fluorescence detection device based on liquid optical device in micro-fluidic chip and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610949502.8A CN106442452A (en) | 2016-11-02 | 2016-11-02 | Fluorescence detection device based on liquid optical device in micro-fluidic chip and detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106442452A true CN106442452A (en) | 2017-02-22 |
Family
ID=58177847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610949502.8A Pending CN106442452A (en) | 2016-11-02 | 2016-11-02 | Fluorescence detection device based on liquid optical device in micro-fluidic chip and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106442452A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271421A (en) * | 2017-07-27 | 2017-10-20 | 深圳中科芯海智能科技有限公司 | A kind of microparticle fluorescence detection means in fluid sample |
| CN107748138A (en) * | 2017-12-05 | 2018-03-02 | 山东交通学院 | Activity of microalgae detection means and method based on filter liquor principle |
| CN108593530A (en) * | 2018-06-01 | 2018-09-28 | 大连海事大学 | Microparticle forward scattering optical detection device and its detection method |
| CN110308086A (en) * | 2019-07-09 | 2019-10-08 | 大连海事大学 | It is a kind of based on micro-fluidic particle surface partial region fluorescent marker device and method |
| CN110661956A (en) * | 2019-10-14 | 2020-01-07 | Oppo广东移动通信有限公司 | Camera module and terminal equipment |
| CN111413301A (en) * | 2019-01-08 | 2020-07-14 | 台达电子国际（新加坡）私人有限公司 | Multicolor fluorescence excitation and detection device |
| CN113418898A (en) * | 2021-06-18 | 2021-09-21 | 贵州医科大学 | Integrated device of PIFO platform and composed of micro-fluidic chip and fluorescence sensor |
| CN113949787A (en) * | 2020-06-30 | 2022-01-18 | 北京小米移动软件有限公司 | Optical filter assembly, lens module, electronic device, imaging method and storage medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102103220A (en) * | 2011-02-28 | 2011-06-22 | 南京邮电大学 | Varifocal lens of micro-fluid control liquid based on ionic liquid |
| CN103234949A (en) * | 2013-01-30 | 2013-08-07 | 大连海事大学 | Microalgae activity detection method and device in ship ballast water |
| CN103529006A (en) * | 2013-10-18 | 2014-01-22 | 大连海事大学 | Portable fluorescence detection device based on micro-fluidic chip and detection method thereof |
-
2016
- 2016-11-02 CN CN201610949502.8A patent/CN106442452A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102103220A (en) * | 2011-02-28 | 2011-06-22 | 南京邮电大学 | Varifocal lens of micro-fluid control liquid based on ionic liquid |
| CN103234949A (en) * | 2013-01-30 | 2013-08-07 | 大连海事大学 | Microalgae activity detection method and device in ship ballast water |
| CN103529006A (en) * | 2013-10-18 | 2014-01-22 | 大连海事大学 | Portable fluorescence detection device based on micro-fluidic chip and detection method thereof |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271421A (en) * | 2017-07-27 | 2017-10-20 | 深圳中科芯海智能科技有限公司 | A kind of microparticle fluorescence detection means in fluid sample |
| CN107748138A (en) * | 2017-12-05 | 2018-03-02 | 山东交通学院 | Activity of microalgae detection means and method based on filter liquor principle |
| CN107748138B (en) * | 2017-12-05 | 2023-11-28 | 山东交通学院 | Microalgae activity detection device and method based on filter liquid principle |
| CN108593530A (en) * | 2018-06-01 | 2018-09-28 | 大连海事大学 | Microparticle forward scattering optical detection device and its detection method |
| CN108593530B (en) * | 2018-06-01 | 2020-10-27 | 大连海事大学 | Microparticle forward scattering light detection device and detection method thereof |
| CN111413301A (en) * | 2019-01-08 | 2020-07-14 | 台达电子国际（新加坡）私人有限公司 | Multicolor fluorescence excitation and detection device |
| CN110308086A (en) * | 2019-07-09 | 2019-10-08 | 大连海事大学 | It is a kind of based on micro-fluidic particle surface partial region fluorescent marker device and method |
| CN110308086B (en) * | 2019-07-09 | 2021-11-05 | 大连海事大学 | Particle surface partial-area fluorescence labeling device and method based on micro-fluidic |
| CN110661956A (en) * | 2019-10-14 | 2020-01-07 | Oppo广东移动通信有限公司 | Camera module and terminal equipment |
| CN113949787A (en) * | 2020-06-30 | 2022-01-18 | 北京小米移动软件有限公司 | Optical filter assembly, lens module, electronic device, imaging method and storage medium |
| CN113949787B (en) * | 2020-06-30 | 2024-01-09 | 北京小米移动软件有限公司 | Optical filter assembly, lens module, electronic device, imaging method and storage medium |
| CN113418898A (en) * | 2021-06-18 | 2021-09-21 | 贵州医科大学 | Integrated device of PIFO platform and composed of micro-fluidic chip and fluorescence sensor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106442452A (en) | Fluorescence detection device based on liquid optical device in micro-fluidic chip and detection method | |
| CN103529006B (en) | A kind of Portable fluorescence based on micro-fluidic chip detection device | |
| US10816456B2 (en) | Systems and methods for reconfigurable point-of-care diagnostics | |
| Barat et al. | Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer | |
| EP1935983B1 (en) | Method for determination of biological particles in blood | |
| AU2012225123B2 (en) | Method and system for portable cell detection and analysis using microfluidic technology | |
| US20140309795A1 (en) | Automated set-up for cell sorting | |
| US7796256B2 (en) | Oil-immersion enhanced imaging flow cytometer | |
| CN1304846C (en) | Micro flow control chip detecting system for flowing cell detection | |
| Testa et al. | Micro flow cytometer with self-aligned 3D hydrodynamic focusing | |
| CN103822868A (en) | Device and method for detecting grain size of phytoplankton in seawater | |
| CN105319197A (en) | Liquid drop micro-fluidic chip based on microlens array | |
| CN109709025B (en) | Multimode imaging optical system | |
| JP2013057547A (en) | Optical measuring apparatus, flow cytometer and optical measuring method | |
| JP2022505446A (en) | Particle sorting modules with alignment windows, systems, and how to use them | |
| Wang et al. | Sheathless microflow cytometer utilizing two bulk standing acoustic waves | |
| CN102353659B (en) | Detector for biochip fluorescent microspectrum and manufacture method thereof | |
| Mohan et al. | A microfluidic flow analyzer with integrated lensed optical fibers | |
| CN102410998A (en) | Laser light scattering (LLS)-Raman spectrum joint device | |
| Camp Jr et al. | Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry | |
| US20090153883A1 (en) | Laminar flow width detecting method, laminar flow width control method, laminar flow control system, and flow cytometer | |
| WO1981001472A1 (en) | Liquid flow photometer | |
| WO2021116959A1 (en) | Analyzing an analyte disposed within a medium | |
| Jiang et al. | Dual-wavelength fluorescent detection of particles on a novel microfluidic chip | |
| Serhatlioglu et al. | Femtosecond laser fabrication of fiber based optofluidic platform for flow cytometry applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170222 |
|
| WD01 | Invention patent application deemed withdrawn after publication |