CN106442451B - Radiation injury assessment device and its appraisal procedure based on micro-fluidic chip - Google Patents
Radiation injury assessment device and its appraisal procedure based on micro-fluidic chip Download PDFInfo
- Publication number
- CN106442451B CN106442451B CN201610945453.0A CN201610945453A CN106442451B CN 106442451 B CN106442451 B CN 106442451B CN 201610945453 A CN201610945453 A CN 201610945453A CN 106442451 B CN106442451 B CN 106442451B
- Authority
- CN
- China
- Prior art keywords
- nematode
- component
- image
- fluorescence
- radiation injury
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of, and device and its working method are assessed in the radiation injury based on micro-fluidic chip, the device includes fluorescent collecting component, light field acquisition component, light source, micro-fluidic chip, data handling component and microcontroller, the micro-fluidic chip is made of substrate and coating, and the coating surface is equipped with culture liquid pool, sample cell, waste liquid pool.The present invention uses the combination of light field acquisition component and fluorescent collecting component, can accurately obtain bright field image, fluorescent image, wavelength of fluorescence and the fluorescence intensity of nematode body, overcome the defect bring error of conventional method.After sample is added dropwise in the present invention, subsequent step is all intelligent completion, therefore, there is no need to the operation of specialized personnel, and step is few, easy to operate, solves the problems, such as that sample treatment is cumbersome.The present invention have many advantages, such as small in size, light weight and cost is low, amount of samples is few, it is easy to carry, be able to carry out and hold for on-site test.
Description
Technical field
The present invention relates to radiation injury assessment technology, device is assessed in especially a kind of radiation injury based on micro-fluidic chip
And its appraisal procedure.
Background technique
With the fast development of aviation industry, the following Chinese Space station needs to be appointed by long-term in-orbit spacefarer's performance of work
Business.Space environment is that have the compound extreme environments such as complicated radiation and microgravity, and space radiation is each comprising atomic number 1-92
The particle radiation of elemental composition, energy is from several electron volts to more than 1022 electron volts.In order to guarantee the physical safety of astronaut
With the research of the living matter damage and hereditary variation that generate to radiation, radiation injury assessment in space has become aerospace medicine neck
Primary problem in domain.Indicate that biotest methodology is a kind of method for carrying out space radiation injury assessment by instruction biology, and
Beautiful hidden main line worm has the mutant of various researchs, is best model organism.
Device currently used for space radiation injury assessment mainly has: commercialization flow cytometer and Laser Scanning Confocal Microscope.
Flow cytometer is a kind of hygiene medical treatment instrument, be mainly used for in cell suspension cell or organelle carry out it is fast
Speed measurement measures the physical characteristic and biochemical characteristic of cell by collecting and analyzing for the light that generates to measurement zone and electric signal,
There is very extensive use in fields such as cell biology, immunology, oncology, hematology, science of heredity and pathology clinical detections
On the way.
Laser Scanning Confocal Microscope is advanced using laser scanning co-focusing microscopy, laser scanning co-focusing microscopy
Molecule and RESEARCH ON CELL-BIOLOGY technology.It installs laser scanning device, combined data additional on the basis of fluorescent microscopic imaging
Image processing techniques acquires tissue and intracellular Fluorescence tag image, observes the variation of calcium plasma level in subcellsular level,
And combine the correlation of the technologies such as electro physiology observation cellular physiological events and cellular morphology and motion change.It is extensive due to it
Using, it has also become critically important research skill in the research fields such as morphology, molecular cytobiology, Neuscience and pharmacology
Art.
To sum up analyze, space radiation injury at present assess most of method there is can only to cell or unicellular organism into
Row measurement, the problem of cannot assessing the radiation injury of multicellular organism, and these apparatus structures are huge, structure is multiple
It is miscellaneous, complicated for operation, expensive, cannot integrate it is portable, can not on-site test.
Summary of the invention
The purpose of the present invention is to overcome the shortcomings of the existing technology and deficiency, and providing one kind can be to the spoke of multicellular organism
Damage is penetrated to be assessed, but structure it is simple, easy to operate, cheap, easy to carry carry out on-site test based on micro-fluidic
The radiation injury assessment device and its appraisal procedure of chip.
To achieve the goals above, technical scheme is as follows:
Device is assessed in radiation injury based on micro-fluidic chip, including fluorescent collecting component, light field acquisition component, light source,
Micro-fluidic chip, power supply power supply component, data handling component and microcontroller, the micro-fluidic chip respectively with fluorescent collecting group
Input terminal, the input terminal of light field acquisition component of part are connected with the output end of microcontroller, the data handling component respectively with
Fluorescent collecting component is connected with the output end of light field acquisition component；The light source is connect with fluorescent collecting component；The power supply supplies
Electrical component is connected with the input terminal of light source, microcontroller and data handling component respectively；
The fluorescent collecting component includes fluorescence microscopy object lens, dichronic mirror, green color filter, plano-convex lens, biconvex lens
With fluorescence detection component, the fluorescence microscopy object lens by optical path successively with dichronic mirror, green color filter, plano-convex lens, biconvex
Lens and the series connection of fluorescence detection component, fluorescent collecting component is for obtaining the fluorescent image, wavelength of fluorescence and fluorescence intensity of nematode；
The light field acquisition component includes microcobjective, plano-convex lens, biconvex lens and CCD, and the microcobjective passes through
Optical path is successively connected with plano-convex lens, biconvex lens and CCD, and light field acquisition component is for obtaining the bright field image of nematode；
The microcontroller is used to control the size and Orientation of electric field force；
The micro-fluidic chip is made of substrate and the coating being fixed on substrate, and the substrate is sheet glass, the painting
Layer is dimethyl silicone polymer coating, and the coating surface is equipped with culture liquid pool, sample cell, waste liquid pool；Liquid pool is cultivated by leading to
Road I is connected with sample cell, and sample cell is connected by channel II with waste liquid pool；Cultivate liquid pool and waste liquid pool respectively with microcontroller phase
Even；
The channel II is gradually transitions narrow sense channel by width from sample cell to waste liquid pool, and narrow end size is less than
Nematode minimum dimension.
Power supply power supply component of the present invention is whole device power supply.
Data handling component of the present invention is for handling bright field image, fluorescent image, wavelength of fluorescence and fluorescence intensity；Number
The surface of nematode body is obtained by bright field image according to processing component, surface includes nematode contour area, aspect ratio
With twisting frequency；Data handling component obtains the luminescence feature of nematode body by fluorescent image, and luminescence feature includes nematode hair
The gray value of photo-site, all luminescence sites total sum of the grayscale values luminescence sites number；Data handling component passes through light field figure
Both picture and fluorescent image, which combine, obtains the distribution characters of nematode luminescence sites, the gray value on unit area.
The appraisal procedure of radiation injury assessment device based on micro-fluidic chip, includes the following steps:
A, sample cell is added in nematode, culture liquid pool is added in culture solution；
B, power supply is opened, nematode enters sense channel in microcontroller control electric field power drive sample cell, while by logical
The culture solution of road I update sample cell；
C, data acquisition is carried out to nematode respectively by fluorescent collecting component and light field acquisition component, light field acquisition component obtains
The bright field image of nematode, fluorescent collecting component obtain the fluorescent image, wavelength of fluorescence and fluorescence intensity of nematode, data handling component
The surface of nematode body is obtained by bright field image, surface includes nematode contour area, aspect ratio and twisting frequency；
Data handling component obtains the luminescence feature of nematode body by fluorescent image, and luminescence feature includes the gray scale of nematode luminescence sites
The number of value, total sum of the grayscale values luminescence sites of all luminescence sites；Data handling component is by combining bright field image and fluorescence
Image obtains the distribution character of nematode luminescence sites, the gray value on unit area；
D, after acquisition, microcontroller control electric field force is driven in the reverse direction nematode and returns to sample cell；Pass through data handling component point
Whether analysis obtains enough data collection capacities, if obtaining enough data collection capacities, detection terminates, and goes to step F；Otherwise, turn step
Rapid E；
E, step B for a period of time by nematode culture, is returned again to detect again；
F, data handling component assesses nematode radiation injury degree；Appraisal procedure the following steps are included:
F1, the radiation injury degree that nematode is assessed by wavelength of fluorescence, the shorter radiation injury for representing nematode of wavelength of fluorescence
Degree is more serious；
F2, the radiation injury degree that nematode is assessed by fluorescence intensity, fluorescence intensity represent more by force the radiation injury of nematode
Degree is more serious；
F3, the radiation injury degree that nematode is assessed by the number of luminescence sites, the number of luminescence sites is bigger to represent line
The radiation injury degree of worm is more serious；
F4, the radiation injury degree that nematode is assessed by the gray value of luminescence sites, the bigger spoke for representing nematode of gray value
It is more serious to penetrate degree of injury；The gray value of luminescence sites refers to the grey scale pixel value in fluorescent image at luminescence sites；
F5, the radiation injury degree that nematode is assessed by gray value on unit area, gray value is bigger to represent nematode
Radiation injury degree is more serious；Gray value on unit area refers to the ratio of total gray value and contour area, by fluorescent image
The total gray value for obtaining all luminescence sites of nematode is obtained area, abbreviation contoured surface in nematode image outline by bright field image
Product；
F6, radiation injury position and its radiation injury degree that nematode is assessed by the distribution character of nematode luminescence sites；
The distribution character of luminescence sites is the nematode luminescence sites obtained by fluorescent image and the nematode image wheel that bright field image obtains
Both wide distribution situation for combining the luminescence sites obtained in image outline；
F7, the activity that nematode is assessed by the comprehensive analysis of nematode twisting frequency and aspect ratio two indices；
F8, period locating for nematode is assessed by nematode contour area and aspect ratio two indices comprehensive analysis, when described
Phase includes dauer phase, larval phase and adult stage.
Compared with prior art, the invention has the following beneficial effects:
1, since the present invention uses the combination of light field acquisition component and fluorescent collecting component, light field can accurately be obtained
Image, fluorescent image and wavelength of fluorescence, it is easy to operate, overcome the defect bring error of simple function in conventional method.
2, since the present invention is after nematode is added in sample cell, subsequent step is all intelligent completion, does not need any profession
To change knowledge, therefore, there is no need to the operation of specialized personnel, step is few, and it is easy to operate, solve the problems, such as that sample treatment is cumbersome.
3, due to the microfluidic platform that the present invention is detected as sample emission lesion assessment using micro-fluidic chip, and relevant inspection
The structure type of small volume also can be used in measurement equipment, and accordingly, with respect to existing large-scale detection device, the present invention has volume
It is small, light weight and cost is low, amount of samples is few, it is easy to carry, be able to carry out the advantages that holding for on-site test.
4, the present invention overcomes present in existing radiation injury appraisal procedure can not on-site test, detection device it is expensive,
Sample treatment is cumbersome, more bring universalities of amount of samples and the problems such as error, fundamentally solves radiation injury evaluation problem.
This will have important scientific meaning and realistic price for environmental science, aviation field and medical domain.
5, since the twisting frequency and aspect ratio of nematode can indicate the activity of nematode, the present invention is by twisting nematode
The analysis of frequency and aspect ratio two indices can more accurately assess the activity of nematode.
6, in period where can obtaining nematode due to the contour area of nematode and aspect ratio, the present invention is by taking turns nematode
The long-pending analysis with aspect ratio two indices of profile surface, can more accurately obtain the period where nematode.
Detailed description of the invention
The present invention shares 2 width of attached drawing, in which:
Fig. 1 is assessment apparatus structure schematic diagram of the invention；
Fig. 2 is microfluidic chip structure schematic diagram of the invention.
Wherein: 1, light field acquisition component, 2, fluorescent collecting component, 3, micro-fluidic chip, 4, light source, 5, microcontroller, 6,
Power supply power supply component, 7, data handling component, 8, culture liquid pool, 9, sample cell, 10, waste liquid pool, 11, channel I, 12, channel II
(sense channel).
Specific embodiment
Following non-limiting embodiments can with a person of ordinary skill in the art will more fully understand the present invention, but not with
Any mode limits the present invention.
The present invention is further described with reference to the accompanying drawing.As shown in Figs. 1-2,
Basic functional principle of the invention is as follows:
The present invention is provided with channel I 11 and channel II 12 on micro-fluidic chip 3, and channel I 11 is for updating culture solution
, channel II 12 is that narrow channel is gradually transitions by width, and nematode enters channel II 12 under the driving of electric field force, but channel
II 12 tail ends are narrow, and nematode cannot get through, and also stay in here, convenient for detection；Therefore channel II 12 is sense channel.And
Nematode viability needs the substances such as food, oxygen, therefore culture solution is just to provide these factors, so that nematode can give birth to for a long time
It deposits, convenient for detection.
When being detected in micro-fluidic chip 3, culture solution is added in culture liquid pool 8, nematode is added in sample cell 9, it is micro-
Controller 5 drives the nematode in sample cell 9 to enter sense channel, and is fixed on nematode in sense channel, while updating culture
Liquid, after to be detected, microcontroller 5 is driven in the reverse direction nematode and returns in sample cell 9, and one-time detection finishes.Micro-fluidic chip 3 has
There is the function of making nematode long time cultivation He repeat at periodic or other desired detection.The sense channel of micro-fluidic chip 3 be one from sample cell 9 to
Waste liquid pool 10 is gradually transitions narrow process by width, is driven nematode to enter sense channel under the action of microcontroller 5, is made nematode
It rests in sense channel, and nematode body is unfolded, convenient for handling its feature.
When detection, light source 4 passes through on the dichroic mirror to nematode in light collection component from side irradiation, at this point, light
Becoming the light source of light field acquisition component 1 through nematode, another aspect illumination, which is mapped on nematode, excites nematode to issue fluorescence, therefore
It is the light source of fluorescent collecting component 2, it can be seen that unique distinction of the invention, using a light source 4, dexterously by fluorescence
Acquisition component 2 and light field acquisition component 1 are combined togather.Testing result is divided into two paths of signals: being all the way nematode bright field image
Signal is detected, another way is that nematode fluorescent image and wavelength of fluorescence detect signal, and two ways carries out simultaneously, independently of each other.Together
When obtain same nematode two-way detection signal can be used for analyzing simultaneously, accurately to obtain nematode radiation injury degree.
Voltage is generated between the control of microcontroller 5 culture liquid pool 8 and waste liquid pool 10, by electric field power drive sample cell 9
Any one nematode carries out data acquisition by fluorescent collecting component 2 and light field acquisition component 1 into sense channel, after acquisition,
The control of microcontroller 5 electric field force direction is reversed, and driving nematode returns to sample cell 9, and one-time detection finishes.
1 workflow of light field acquisition component are as follows: light field data amplify again through microcobjective amplification → plano-convex lens → and it is bis-
Convex lens focuses, and → carrying out data acquisition by CCD → is transferred to data handling component 7 and handles.
2 workflow of fluorescent image acquisition component are as follows: nematode generate fluorescence data through fluorescence microscopy object lens amplification → it is green
Colo(u)r filter filter out veiling glare data → plano-convex lens again amplification → biconvex lens focus → data are carried out by fluorescence detection component
Acquire → be transferred to the processing of data handling component 7.
Claims (2)
1. device, including fluorescent collecting component (2), light field acquisition component (1), light are assessed in the radiation injury based on micro-fluidic chip
Source (4), micro-fluidic chip (3), power supply power supply component (6), data handling component (7) and microcontroller (5), the micro-fluidic core
Piece (3) output with the input terminal of fluorescent collecting component (2), the input terminal of light field acquisition component (1) and microcontroller (5) respectively
End connection, the data handling component (7) connect with the output end of fluorescent collecting component (2) and light field acquisition component (1) respectively；
The light source (4) connect with fluorescent collecting component (2)；The power supply power supply component (6) respectively with light source (4), microcontroller (5)
It is connected with the input terminal of data handling component (7)；
The fluorescent collecting component (2) include fluorescence microscopy object lens, dichronic mirror, green color filter, plano-convex lens, biconvex lens and
Fluorescence detection component, the fluorescence microscopy object lens by optical path successively with dichronic mirror, green color filter, plano-convex lens, lenticular
Mirror and the series connection of fluorescence detection component, the fluorescent image, wavelength of fluorescence and fluorescence that fluorescent collecting component (2) is used to obtain nematode are strong
Degree；
The light field acquisition component (1) includes microcobjective, plano-convex lens, biconvex lens and CCD, and the microcobjective passes through light
Road is successively connected with plano-convex lens, biconvex lens and CCD, and light field acquisition component (1) is for obtaining the bright field image of nematode；
The microcontroller (5) is used to control the size and Orientation of electric field force；
The micro-fluidic chip (3) is made of substrate and the coating being fixed on substrate, and the substrate is sheet glass, the coating
For dimethyl silicone polymer coating, the coating surface is equipped with culture liquid pool (8), sample cell (9), waste liquid pool (10)；Culture solution
Pond (8) is connected by channel I (11) with sample cell (9), and sample cell (9) is connected by channel II (12) with waste liquid pool (10)；Training
Nutrient solution pond (8) and waste liquid pool (10) are connected with microcontroller (5) respectively；
The channel II (12) is gradually transitions narrow sense channel, narrow end by width from sample cell (9) to waste liquid pool (10)
Size is less than nematode minimum dimension；
The power supply power supply component (6) is whole device power supply；
It is characterized by: the data handling component (7) is strong for handling bright field image, fluorescent image, wavelength of fluorescence and fluorescence
Degree；Data handling component (7) obtains the surface of nematode body by bright field image, and surface includes nematode contoured surface
Product, aspect ratio and twisting frequency；Data handling component (7) obtains the luminescence feature of nematode body by fluorescent image, and shine spy
Sign include the gray values of nematode luminescence sites, all luminescence sites total sum of the grayscale values luminescence sites number；Data processing group
Part (7) is combined by both bright field image and fluorescent image obtains the distribution characters of nematode luminescence sites, the ash on unit area
Angle value.
2. the appraisal procedure of the radiation injury assessment device based on micro-fluidic chip, characterized by the following steps:
A, sample cell (9) are added in nematode, culture liquid pool (8) is added in culture solution；
B, power supply is opened, nematode enters sense channel in microcontroller (5) control electric field power drive sample cell (9), passes through simultaneously
Channel I updates the culture solution of sample cell (9)；
C, data acquisition, light field acquisition component are carried out to nematode respectively by fluorescent collecting component (2) and light field acquisition component (1)
(1) bright field image of nematode is obtained, fluorescent collecting component (2) obtains the fluorescent image, wavelength of fluorescence and fluorescence intensity of nematode, number
The surface of nematode body is obtained by bright field image according to processing component (7), surface includes nematode contour area, in length and breadth
Than and twisting frequency；Data handling component (7) obtains the luminescence feature of nematode body by fluorescent image, and luminescence feature includes line
The gray value of worm luminescence sites, all luminescence sites total sum of the grayscale values luminescence sites number；Data handling component (7) passes through
The distribution characters of nematode luminescence sites, the gray value on unit area are obtained in conjunction with bright field image and fluorescent image；
D, after acquisition, microcontroller (5) control electric field force is driven in the reverse direction nematode and returns to sample cell (9)；Pass through data handling component
(7) it analyses whether to obtain enough data collection capacities, if obtaining enough data collection capacities, detection terminates, and goes to step F；Otherwise,
Go to step E；
E, step B for a period of time by nematode culture, is returned again to detect again；
F, data handling component (7) assesses nematode radiation injury degree；Appraisal procedure the following steps are included:
F1, the radiation injury degree that nematode is assessed by wavelength of fluorescence, the shorter radiation injury degree for representing nematode of wavelength of fluorescence
It is more serious；
F2, the radiation injury degree that nematode is assessed by fluorescence intensity, fluorescence intensity represent more by force the radiation injury degree of nematode
It is more serious；
F3, the radiation injury degree that nematode is assessed by the numbers of luminescence sites, the number of luminescence sites is bigger to represent nematode
Radiation injury degree is more serious；
F4, the radiation injury degree that nematode is assessed by the gray value of luminescence sites, the bigger radiation damage for representing nematode of gray value
It is more serious to hurt degree；The gray value of luminescence sites refers to the grey scale pixel value in fluorescent image at luminescence sites；
F5, the radiation injury degree that nematode is assessed by the gray value on unit area, the bigger radiation for representing nematode of gray value
Degree of injury is more serious；Gray value on unit area refers to the ratio of total gray value and contour area, is obtained by fluorescent image
Total gray value of all luminescence sites of nematode is obtained area, abbreviation contour area in nematode image outline by bright field image；
F6, radiation injury position and its radiation injury degree that nematode is assessed by the distribution character of nematode luminescence sites；It shines
The distribution character in site is the nematode luminescence sites obtained by fluorescent image and the nematode image outline two that bright field image obtains
Person combines distribution situation of the luminescence sites obtained in image outline；
F7, the activity that nematode is assessed by the comprehensive analysis of nematode twisting frequency and aspect ratio two indices；
F8, period locating for nematode, the period packet are assessed by nematode contour area and aspect ratio two indices comprehensive analysis
Include dauer phase, larval phase and adult stage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610945453.0A CN106442451B (en) | 2016-11-02 | 2016-11-02 | Radiation injury assessment device and its appraisal procedure based on micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610945453.0A CN106442451B (en) | 2016-11-02 | 2016-11-02 | Radiation injury assessment device and its appraisal procedure based on micro-fluidic chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106442451A CN106442451A (en) | 2017-02-22 |
| CN106442451B true CN106442451B (en) | 2019-03-26 |
Family
ID=58178386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610945453.0A Active CN106442451B (en) | 2016-11-02 | 2016-11-02 | Radiation injury assessment device and its appraisal procedure based on micro-fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106442451B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107228847A (en) * | 2017-05-18 | 2017-10-03 | 大连海事大学 | The micro-fluidic radiation injury monitoring device and method of collection capture culture detection integration |
| CN110501039A (en) * | 2018-05-16 | 2019-11-26 | 上海医药工业研究院 | Nematode microfluidic chip analysis device |
| CN109187463B (en) * | 2018-08-31 | 2020-12-01 | 哈尔滨工业大学 | Microfluidic radiation damage biological dose analysis and detection device based on luminescent bacteria and detection and analysis method thereof |
| CN109975258A (en) * | 2019-03-25 | 2019-07-05 | 武汉理工大学 | A kind of micro-fluidic detection system of signal enhancing |
| CN113340921B (en) * | 2021-02-01 | 2022-11-01 | 中国人民解放军军事科学院军事医学研究院 | Quantitative evaluation method and evaluation system for electromagnetic radiation damage and electromagnetic radiation biological effect |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212705A (en) * | 2014-09-19 | 2014-12-17 | 成都劲宏科技有限公司 | Pulse detection technology based dual-passage cell microflow image acquisition system |
| CN205199533U (en) * | 2015-12-17 | 2016-05-04 | 广州万孚生物技术股份有限公司 | Micro -fluidic chip for detection |
-
2016
- 2016-11-02 CN CN201610945453.0A patent/CN106442451B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212705A (en) * | 2014-09-19 | 2014-12-17 | 成都劲宏科技有限公司 | Pulse detection technology based dual-passage cell microflow image acquisition system |
| CN205199533U (en) * | 2015-12-17 | 2016-05-04 | 广州万孚生物技术股份有限公司 | Micro -fluidic chip for detection |
Non-Patent Citations (2)
| Title |
|---|
| "基于开放式微流控芯片的线虫细胞钙信号研究";赵幸福;《中国博士学位论文全文数据库 基础科学辑》;20150215(第02期);论文正文第63-64页 |
| "基于微流控芯片的细胞辐射损伤检测系统设计";宋文东;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20160215(第02期);论文摘要，论文正文第11、23-28、38、43页 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106442451A (en) | 2017-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106442451B (en) | Radiation injury assessment device and its appraisal procedure based on micro-fluidic chip | |
| Kohen | Cell structure and function by microspectrofluorometry | |
| Schroeder | Imaging stem-cell-driven regeneration in mammals | |
| Michalet et al. | The power and prospects of fluorescence microscopies and spectroscopies | |
| Pantazis et al. | Advances in whole-embryo imaging: a quantitative transition is underway | |
| Schaap et al. | Lab on a chip technologies for algae detection: a review | |
| JP7105483B2 (en) | Data creation method and data usage method | |
| Ramanujan et al. | Multiphoton fluorescence lifetime contrast<? xpp qa?> in deep tissue imaging: prospects in redox imaging<? xpp qa?> and disease diagnosis | |
| Cutrale et al. | Imaging, visualization, and computation in developmental biology | |
| Liu et al. | The application of spectroscopy technology in the monitoring of microalgae cells concentration | |
| CN202110128U (en) | Photonic crystal coding microsphere biochip detection device | |
| CN216082488U (en) | Fold line light path device capable of switching double irradiation channels through multi-power objective lens | |
| CN105424667B (en) | Trace method for rapid inspecting animalcule | |
| Ameer-Beg et al. | Special issue on fluorescence lifetime imaging (FLIM): from fundamentals to applications | |
| CN204832040U (en) | Nanometer OCT imaging system based on broadband stimulated emission | |
| Yu et al. | Estimation of fluorescence lifetimes via rotational invariance techniques | |
| Ding et al. | An innovative miniaturized cell imaging system based on integrated coaxial dual optical path structure and microfluidic fixed frequency sample loading strategy | |
| Seruca et al. | Fluorescence imaging and biological quantification | |
| Welling et al. | Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches | |
| Walsh et al. | Fluorescence lifetime imaging of calcium flux in neurons in response to pulsed infrared light | |
| US20240151691A1 (en) | Method and apparatus for illuminating and imaging organoids and spheroids | |
| Rosa et al. | Two-photon photoactivation to measure histone exchange dynamics in plant root cells | |
| Baiazitova et al. | Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells | |
| Stoltzfus et al. | Optimizing ultrafast wide field-of-view illumination for high-throughput multi-photon imaging and screening of mutant fluorescent proteins | |
| Guo et al. | High-throughput optofluidic profiling of Euglena gracilis with morphological and chemical specificity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220121 Address after: 116026 No. 1, Ling Hai Road, Liaoning, Dalian Patentee after: Dalian Maritime University Patentee after: Shanghai Shipping Research Institute Co., Ltd Address before: 116026 1 Linghai Road, hi tech park, Dalian, Liaoning Patentee before: Dalian Maritime University |
|
| TR01 | Transfer of patent right |