CN106442375A - Florfenicol rapid test kit - Google Patents
Florfenicol rapid test kit Download PDFInfo
- Publication number
- CN106442375A CN106442375A CN201611020696.XA CN201611020696A CN106442375A CN 106442375 A CN106442375 A CN 106442375A CN 201611020696 A CN201611020696 A CN 201611020696A CN 106442375 A CN106442375 A CN 106442375A
- Authority
- CN
- China
- Prior art keywords
- florfenicol
- cell
- detection cell
- test kit
- dissolving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- AYIRNRDRBQJXIF-NXEZZACHSA-N (-)-Florfenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CF)NC(=O)C(Cl)Cl)C=C1 AYIRNRDRBQJXIF-NXEZZACHSA-N 0.000 title claims abstract description 76
- 229960003760 florfenicol Drugs 0.000 title claims abstract description 75
- 238000001514 detection method Methods 0.000 claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 9
- 230000006698 induction Effects 0.000 claims description 5
- 239000003292 glue Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 2
- PZUUTJLAUKMLTH-UHFFFAOYSA-N [F].C1=CC=CC=C1 Chemical compound [F].C1=CC=CC=C1 PZUUTJLAUKMLTH-UHFFFAOYSA-N 0.000 claims 1
- PYLWMHQQBFSUBP-UHFFFAOYSA-N monofluorobenzene Chemical compound FC1=CC=CC=C1 PYLWMHQQBFSUBP-UHFFFAOYSA-N 0.000 claims 1
- 238000004090 dissolution Methods 0.000 abstract 6
- 210000004027 cell Anatomy 0.000 description 28
- 239000003814 drug Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000606860 Pasteurella Species 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 229940097572 chloromycetin Drugs 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- TYCOFFBAZNSQOJ-UHFFFAOYSA-N 2-[4-(3-fluorophenyl)phenyl]propanoic acid Chemical compound C1=CC(C(C(O)=O)C)=CC=C1C1=CC=CC(F)=C1 TYCOFFBAZNSQOJ-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000606748 Actinobacillus pleuropneumoniae Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101000984722 Bos taurus Pancreatic trypsin inhibitor Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 206010017553 Furuncle Diseases 0.000 description 1
- 241000606790 Haemophilus Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 125000000266 alpha-aminoacyl group Chemical group 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 229950001284 fluprofen Drugs 0.000 description 1
- 208000003512 furunculosis Diseases 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000651 prodrug Substances 0.000 description 1
- 229940002612 prodrug Drugs 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 210000004708 ribosome subunit Anatomy 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- OTVAEFIXJLOWRX-NXEZZACHSA-N thiamphenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CO)NC(=O)C(Cl)Cl)C=C1 OTVAEFIXJLOWRX-NXEZZACHSA-N 0.000 description 1
- 229960003053 thiamphenicol Drugs 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Abstract
The invention discloses florfenicol rapid test kit, including a test kit box, a florfenicol dissolution detection cell and a florfenicol extraction measuring cell, the test kit box inside is fixedly provided with the florfenicol dissolution detection cell and the florfenicol extraction measuring cell, one side of the florfenicol dissolution detection cell is provided with an outflow tube, the outflow tube is controlled by a valve, the valve is provided above the florfenicol dissolution detection cell, the edge of the test kit box is fixedly provided with an ultra violate ultraviolet spectrophotometer and a high performance liquid chromatograph, the high performance liquid chromatograph atop is provided with a sensor and a warning lamp, right on the two ends of the florfenicol dissolution detection cell, the florfenicol dissolution detection cell through the outflow tube connects the the florfenicol extraction measuring cell, one section of the test kit box through a concave groove is fixedly provided with a rubber head dropping pipette, a capillary pipette and a stirring rod. The florfenicol rapid test kit not only can detect the existence of florfenicol by safe means, but also can measure the content of florfenicol.
Description
Technical field
The present invention relates to florfenicol quick detection kit technical field, specially a kind of florfenicol quick detection examination
Agent box.
Background technology
Florfenicol（Florfenicol）Chinese：Fluprofen；Florfenicol；Florfenicol, be in 80 years
The broad spectrum antibiotic of the special chloromycetin of a kind of new veterinary that successfully develops for the later stage, nineteen ninety first in Japan's listing,
Norway in 1993 ratifies the furunculosis that salmon treated by the medicine, nineteen ninety-five France, Britain, Austria, Mexico and Spain ratifies to be used for
Treatment cattle respiratory system bacterial disease.Also ratify the feed additive as pig in Japan and Mexico, prevent and treat pig
Bacterial disease（Qiu Yinsheng etc., 1996）, China has passed through the examination ＆ approval of the medicine at present.This product is the derivative of florfenicol
Thing, the principle design according to prodrug is formed, and it all has work to multiple gram positive bacterias and gram negative bacteria and mycoplasma etc.
With.Crucial BPTI acyl of its Antibacterial Mechanism mainly by being combined with 50S ribosomal subunit, needed for suppression protein synthesis
Transferring enzyme, so as to specifically prevent the receptor binding on aminoacyl tRNA and ribosome, suppresses the prolongation of peptide chain and makes thalline egg
Can not synthesize in vain.The MIC50 and MIC90 of bovine respiratory disease pathogen is respectively（μg/ml）：Pasteurella hemolytica
0.50,1.00；Killing property pasteurella 0.50,0.50 more；MIC to actinobacillus pleuropneumoniae is 0.2-1.56 μ g/
ml.Shigella dysenteriae, salmonella typhi, escherichia coli and resistance to ampicillin influenza to resistance to chloromycetin and resistance to thiamphenicol
The MIC of haemophiluss is 0.5 μ g/ml.
Conventional florfenicol quick detection kit can only detect florfenicol presence by reagent, and required time is long,
Medicament is wasted, and does not measure the content of the consuming of the florfenicol detection kit in florfenicol solvent.
Content of the invention
It is an object of the invention to provide a kind of florfenicol quick detection kit, the content for possessing measurement florfenicol
Function, solve that the detection of conventional florfenicol detection kit is slow, waste medicament, it is impossible to the problem of recovery.
For achieving the above object, the present invention provides following technical scheme：
A kind of florfenicol quick detection kit, carries including test agent box body, florfenicol dissolving detection cell and florfenicol
Measuring cell is taken, the test agent tray interior is fixed with florfenicol dissolving detection cell and florfenicol extracts measuring cell, institute
State florfenicol dissolving detection cell side and outlet tube is provided with, the outlet tube is by Valve controlling, and the valve is arranged on benzene Buddhist nun and examines
The top of dissolving detection cell, the edge of the test agent box body is fixed with UV detector and high performance liquid chromatograph,
Induction apparatuss and alarm lamp is provided with the high performance liquid chromatograph, is just arranged on the two ends that florfenicol dissolves detection cell,
The florfenicol dissolving detection cell connects florfenicol by outlet tube and extracts measuring cell, and a section of the test agent box body leads to
Cross groove and be fixed with glue head dropper, capillary pipette and stirring rod.
Preferably, the florfenicol dissolving detection cell is clear glass pond.
Preferably, the florfenicol is extracted in measuring cell and is provided with KCL solution.
Preferably, the lower section of the florfenicol extraction measuring cell is provided with zone of heating, is provided with heating wire in zone of heating.
Compared with prior art, beneficial effects of the present invention are as follows：
1st, this kind of invention takes and uses optical detection, safe and efficient, has saved the time, it is ensured that the safety of experimenter.
2nd, this kind of invention can obtain florfenicol to easily extracting after detection, it is to avoid waste.
Description of the drawings
Fig. 1 is a kind of overlooking the structure diagram of florfenicol quick detection kit of the present invention；
Fig. 2 is that in a kind of florfenicol quick detection reagent of the present invention, florfenicol dissolving detection cell and florfenicol extract measurement
The structural representation in pond.
In figure：1- test agent box body, 2- glue head dropper, 3- capillary pipette, 4- stirring rod, 5- UV detector, 6-
Benzene Buddhist nun examines dissolving detection cell, 7- high performance liquid chromatograph, 8- alarm lamp, and 9- florfenicol extracts measuring cell, 10- valve, and 11- is defeated
Outlet pipe, 12- zone of heating, 13- induction apparatuss, 14- heating wire.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1-2 is referred to, the present invention provides a kind of technical scheme：A kind of florfenicol quick detection kit, including surveying
Reagent box body 1, florfenicol dissolving detection cell 6 and florfenicol extract measuring cell 9, and the internal fixation of the test agent box body 1 sets
Florfenicol dissolving detection cell 6 and florfenicol is had to extract measuring cell 9, florfenicol dissolving 6 side of detection cell is provided with defeated
Outlet pipe 11, the outlet tube 11 is controlled by valve 10, and the valve 10 is arranged on the top that benzene Buddhist nun examines dissolving detection cell 6, described
The edge of test agent box body 1 is fixed with UV detector 5 and high performance liquid chromatograph 7, the high performance liquid chromatograph 7
On be provided with induction apparatuss 13 and alarm lamp 8, be just arranged on the two ends that florfenicol dissolves detection cell 6, the florfenicol is molten
Solution detection cell 6 connects florfenicol by outlet tube 11 and extracts measuring cell 9, and a section of the test agent box body 1 is solid by groove
Glue head dropper 2, capillary pipette 3 and stirring rod 4 are provided with surely.
Operation principle：Dissolved in detection cell 6 by detection object is dissolved in florfenicol, start ultraviolet spectrometry light afterwards
Degree instrument 5 and high performance liquid chromatograph 7, in the presence of induction apparatuss, send alarm, experimenter if containing florfenicol in solution
Will valve 10 open, solution enters florfenicol and extracts in measuring cell 9, and florfenicol and florfenicol are extracted in measuring cell 9
Metal cation reaction after formed complex, afterwards florfenicol extract measuring cell 9 lower section be provided with zone of heating 12, zone of heating
Heated solution in 12, obtains the complex of florfenicol and metal cation, you can to measure solution after evaporating moisture
The content of middle florfenicol.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, permissible
Understanding can carry out multiple changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (4)
1. a kind of florfenicol quick detection kit, including test agent box body（1）, florfenicol dissolving detection cell（6）And fluorine
Benzene Buddhist nun examines extraction measuring cell（9）, the test agent box body（1）Inside is fixed with florfenicol dissolving detection cell（6）And fluorobenzene
Buddhist nun examines extraction measuring cell（9）, the florfenicol dissolving detection cell（6）Side is provided with outlet tube（11）, the outlet tube（11）
By valve（10）Control, valve（10）It is arranged on benzene Buddhist nun and examines dissolving detection cell（6）Top, the test agent box body（1）Side
Edge is fixed with UV detector（5）And high performance liquid chromatograph（7）, the high performance liquid chromatograph（7）On be provided with
Induction apparatuss（13）And alarm lamp（8）, just it is arranged on florfenicol dissolving detection cell（6）Two ends, florfenicol dissolving
Detection cell（6）By outlet tube（11）Connection florfenicol extracts measuring cell（9）, the test agent box body（1）One section pass through
Groove is fixed with glue head dropper（2）, capillary pipette（3）And stirring rod（4）.
2. a kind of florfenicol quick detection kit according to claim 1, it is characterised in that：The florfenicol is molten
Solution detection cell（6）For clear glass pond.
3. a kind of florfenicol quick detection kit according to claim 1, it is characterised in that：The florfenicol is carried
Take measuring cell（9）KCL solution is inside provided with.
4. a kind of florfenicol quick detection kit according to claim 1, it is characterised in that：The florfenicol is carried
Take measuring cell（9）Lower section be provided with zone of heating（12）, zone of heating（12）Heating wire is inside provided with（14）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611020696.XA CN106442375A (en) | 2016-11-21 | 2016-11-21 | Florfenicol rapid test kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611020696.XA CN106442375A (en) | 2016-11-21 | 2016-11-21 | Florfenicol rapid test kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106442375A true CN106442375A (en) | 2017-02-22 |
Family
ID=58220800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611020696.XA Pending CN106442375A (en) | 2016-11-21 | 2016-11-21 | Florfenicol rapid test kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106442375A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687756A (en) * | 2004-12-27 | 2005-10-26 | 南京农业大学 | Kit for testing pesticide residue rapidly and use method thereof |
| CN101299045A (en) * | 2008-06-12 | 2008-11-05 | 中国农业大学 | Method for detecting florfenicol and florfenicol amine and special-purpose enzyme-linked immunologic reagent kit thereof |
| CN101349693A (en) * | 2008-08-25 | 2009-01-21 | 深圳市绿诗源生物技术有限公司 | Fluorobenzene niekau series medicament fast detecting reagent kit and uses thereof |
| CN204142635U (en) * | 2014-07-18 | 2015-02-04 | 青岛贝尔奥生物科技有限公司 | A kind of detection mucous membrane of rectum cell free ferroprotoporphyrin quick detection kit |
| CN105973888A (en) * | 2016-07-21 | 2016-09-28 | 韩雅君 | Kit for rapidly detecting ischemia modified albumin in blood |
-
2016
- 2016-11-21 CN CN201611020696.XA patent/CN106442375A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687756A (en) * | 2004-12-27 | 2005-10-26 | 南京农业大学 | Kit for testing pesticide residue rapidly and use method thereof |
| CN101299045A (en) * | 2008-06-12 | 2008-11-05 | 中国农业大学 | Method for detecting florfenicol and florfenicol amine and special-purpose enzyme-linked immunologic reagent kit thereof |
| CN101349693A (en) * | 2008-08-25 | 2009-01-21 | 深圳市绿诗源生物技术有限公司 | Fluorobenzene niekau series medicament fast detecting reagent kit and uses thereof |
| CN204142635U (en) * | 2014-07-18 | 2015-02-04 | 青岛贝尔奥生物科技有限公司 | A kind of detection mucous membrane of rectum cell free ferroprotoporphyrin quick detection kit |
| CN105973888A (en) * | 2016-07-21 | 2016-09-28 | 韩雅君 | Kit for rapidly detecting ischemia modified albumin in blood |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170222 |